FastQCFastQC Report
Thu 9 Jul 2015
000000000-AG3CH l01n01 80.3410000000e12d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG3CH l01n01 80.3410000000e12d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270195
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATATC38350.015.880052145
GCCGTCT11000.015.159091145
TATGCCG12950.015.115829145
TCGTATG20050.014.4638405145
TCTCGTA19350.014.237726145
TATCTCG23200.014.062501145
CTCGTAT17750.013.478873145
CGCCAAT47900.012.865344145
CACGCCA60350.012.854184145
ATCTCGT23350.012.730192145
TCACGCC68750.012.443637145
CAGTCAC83850.012.191413145
AGTCACG71800.011.915042145
AATATCT38900.011.741646145
ACGCCAA54900.011.489071145
ATATCTC31600.011.242088145
GTCACGC67550.011.054774145
AACTCCA123200.011.004464145
CCAGTCA88600.010.801354145
TCCAGTC96250.010.545454145