FastQCFastQC Report
Thu 9 Jul 2015
000000000-AG3CH l01n01 130.3410000000e1e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG3CH l01n01 130.3410000000e1e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences918259
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT8000.013.59375145
TGCCGTC9200.013.396738145
TATGCCG10800.012.754629145
ACAGTGA34800.012.500001145
CCGTCTT8806.548362E-1112.357954145
ATCTCGT16050.012.196262145
GTCACAC42900.012.167831145
CAGTCAC50100.011.576847145
CAGTGAT29400.010.357142145
TCTCGTA14100.010.283689145
AGTGATC27000.010.203704145
TCACACA46250.010.189189145
ATGCCGT9650.09.766839145
CTCCAGT61650.09.760746145
GAACTCC77700.09.6106825145
GATCTCG18200.09.560439145
TCGTATG13700.09.525548145
GTGATCT25000.08.99145
CTCGTAT13100.08.854961140-144
ACACAGT38650.08.816299145