##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AG1W8 l01n01 mis24.3410000000d2f6.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	900415
Sequences flagged as poor quality	0
Sequence length	151
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.96069034833938	34.0	34.0	34.0	34.0	34.0
2	33.32389620341731	34.0	34.0	34.0	34.0	34.0
3	33.87834720656586	34.0	34.0	34.0	34.0	34.0
4	33.81281520187913	34.0	34.0	34.0	34.0	34.0
5	33.77373544421184	34.0	34.0	34.0	34.0	34.0
6	36.3486192477913	38.0	38.0	38.0	34.0	38.0
7	37.19159942915211	38.0	38.0	38.0	36.0	38.0
8	36.77407417690731	38.0	38.0	38.0	34.0	38.0
9	37.218202717635755	38.0	38.0	38.0	36.0	38.0
10-14	37.155490523814024	38.0	38.0	38.0	35.8	38.0
15-19	37.31730279926478	38.0	38.0	38.0	36.6	38.0
20-24	37.439282775164784	38.0	38.0	38.0	36.8	38.0
25-29	37.19576950628321	38.0	38.0	38.0	36.0	38.0
30-34	36.91007013432695	38.0	38.0	38.0	35.2	38.0
35-39	37.14242032840413	38.0	38.0	38.0	36.2	38.0
40-44	37.11763309140785	38.0	38.0	38.0	36.2	38.0
45-49	37.07600450903195	38.0	38.0	38.0	36.2	38.0
50-54	36.98509598351871	38.0	38.0	38.0	35.6	38.0
55-59	36.516048710872205	38.0	37.4	38.0	34.2	38.0
60-64	36.66955037399421	38.0	37.2	38.0	34.2	38.0
65-69	36.66340987211453	38.0	37.0	38.0	34.2	38.0
70-74	36.78107628149242	38.0	37.8	38.0	35.0	38.0
75-79	36.42138813769206	38.0	37.2	38.0	33.8	38.0
80-84	36.66279282330925	38.0	37.0	38.0	34.4	38.0
85-89	36.57480495105035	38.0	37.0	38.0	34.0	38.0
90-94	36.40529422544049	38.0	37.0	38.0	34.0	38.0
95-99	36.37233475675106	38.0	37.0	38.0	33.6	38.0
100-104	35.884314010761706	38.0	36.6	38.0	31.4	38.0
105-109	35.72196842567039	38.0	36.6	38.0	30.6	38.0
110-114	36.00265566433255	38.0	37.0	38.0	32.6	38.0
115-119	35.573254554844155	38.0	36.4	38.0	30.2	38.0
120-124	35.86413176146554	38.0	36.6	38.0	31.4	38.0
125-129	35.5587945558437	38.0	36.8	38.0	30.4	38.0
130-134	35.31760466007341	38.0	36.0	38.0	28.8	38.0
135-139	34.79418357090897	38.0	35.2	38.0	26.2	38.0
140-144	35.37672184492706	38.0	36.2	38.0	29.2	38.0
145-149	34.78616771155522	37.6	35.2	38.0	26.0	38.0
150-151	32.88545559547542	36.0	31.0	37.5	22.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7853054574032043
1101	2	0.5266180208267386
1101	3	0.0798517575740263
1101	4	0.032731016211499764
1101	5	0.14773750657263207
1101	6	1.2211968877592554
1101	7	0.4053010824533203
1101	8	0.45884678955294333
1101	9	0.2645268241573504
1101	10-14	0.34634674638807184
1101	15-19	0.2983814977264174
1101	20-24	0.21619859012545106
1101	25-29	0.2643588030527937
1101	30-34	0.32975737955501216
1101	35-39	0.21908375301067906
1101	40-44	0.28479023538027803
1101	45-49	0.3032140849713656
1101	50-54	0.25164682640171065
1101	55-59	0.4178749445657246
1101	60-64	0.47015271654310453
1101	65-69	0.35514240507174577
1101	70-74	0.3411868391536217
1101	75-79	0.3721224022518186
1101	80-84	0.37515997529523304
1101	85-89	0.5348982818394674
1101	90-94	0.46861379908918366
1101	95-99	0.48668035229765394
1101	100-104	0.7816178169093959
1101	105-109	0.6418437557153496
1101	110-114	0.40220203564005175
1101	115-119	0.7912365038721418
1101	120-124	0.6021910565533162
1101	125-129	0.47251198727882127
1101	130-134	0.7235832142143792
1101	135-139	0.778254389564907
1101	140-144	0.6424393770537549
1101	145-149	0.7813014553684781
1101	150-151	0.4984663780780849
1102	1	0.869145729033967
1102	2	0.3530507025084546
1102	3	0.06528763204651256
1102	4	0.05008786362245132
1102	5	0.14310872773378236
1102	6	1.0479533561036973
1102	7	0.451163374549175
1102	8	0.23208715103821476
1102	9	0.19687704145436413
1102	10-14	0.2527563188493289
1102	15-19	0.20424527253733515
1102	20-24	0.13818264780141476
1102	25-29	0.23197782519726218
1102	30-34	0.2041670880694113
1102	35-39	0.1861173503762643
1102	40-44	0.2240720630230797
1102	45-49	0.2022521529375041
1102	50-54	0.12457840999918801
1102	55-59	0.2950607645921792
1102	60-64	0.3782602454168327
1102	65-69	0.3297503317853412
1102	70-74	0.2802926893231259
1102	75-79	0.267977883434952
1102	80-84	0.34635798759846637
1102	85-89	0.31972905005923025
1102	90-94	0.42462101584973766
1102	95-99	0.3884280902903541
1102	100-104	0.7387661298587105
1102	105-109	0.4509514568019739
1102	110-114	0.2952604029330175
1102	115-119	0.6498050365968098
1102	120-124	0.5182617889963907
1102	125-129	0.5318872622952213
1102	130-134	0.5945245583038385
1102	135-139	0.6905667860699864
1102	140-144	0.46923550554934934
1102	145-149	0.7970469316428108
1102	150-151	1.1402277246871648
1103	1	0.8281928341873552
1103	2	0.3703520770188504
1103	3	0.07287705714568915
1103	4	0.03279104947736755
1103	5	0.1388993476812388
1103	6	1.2666637531991398
1103	7	0.4032283975407438
1103	8	0.30144086095239686
1103	9	0.18937019154135726
1103	10-14	0.24940379886474773
1103	15-19	0.29838023557149995
1103	20-24	0.16715458347615453
1103	25-29	0.24739511735332087
1103	30-34	0.39429197530215987
1103	35-39	0.2233951771146323
1103	40-44	0.24271115554946476
1103	45-49	0.23622514808283057
1103	50-54	0.3055461488348854
1103	55-59	0.46814832246607097
1103	60-64	0.48235846656490367
1103	65-69	0.4167921677623525
1103	70-74	0.43014743354444107
1103	75-79	0.35914450468798975
1103	80-84	0.44938449032433425
1103	85-89	0.39514459298946036
1103	90-94	0.4791882624265753
1103	95-99	0.5030915377734289
1103	100-104	0.7369093771221955
1103	105-109	0.6412744267594306
1103	110-114	0.5617791943254602
1103	115-119	0.9622030248893765
1103	120-124	0.8057235368964655
1103	125-129	0.5446150464348918
1103	130-134	0.8055885583077398
1103	135-139	0.8540265006986161
1103	140-144	0.9533150585162886
1103	145-149	1.1537544065213083
1103	150-151	1.1004395145670358
2116	1	0.6383946244984102
2116	2	0.2418125250637999
2116	3	-0.059138639706809215
2116	4	-0.24650226296716937
2116	5	0.024730067006153433
2116	6	1.033624548808703
2116	7	-0.002860242824063164
2116	8	0.24099587516838028
2116	9	0.09067214979071281
2116	10-14	0.1864189728324348
2116	15-19	0.18509428940187433
2116	20-24	0.10559140560670244
2116	25-29	0.20593143224402866
2116	30-34	0.34470699953691764
2116	35-39	0.15977261449356206
2116	40-44	0.07040845561834885
2116	45-49	0.19358198779028868
2116	50-54	0.12850589634719256
2116	55-59	0.33665953389790815
2116	60-64	0.36096609330129326
2116	65-69	0.35760479893037456
2116	70-74	0.25953262907093944
2116	75-79	0.3416399504360683
2116	80-84	0.41915227127599763
2116	85-89	0.40438776668247556
2116	90-94	0.4492644539763191
2116	95-99	0.5202622105358188
2116	100-104	0.7129847949021695
2116	105-109	0.5462579928804132
2116	110-114	0.37145826608105637
2116	115-119	0.7683517845384316
2116	120-124	0.6303230644879037
2116	125-129	0.6532115886782535
2116	130-134	0.7416351733642728
2116	135-139	0.9288773704580677
2116	140-144	0.9473665413062164
2116	145-149	1.053460244326594
2116	150-151	1.579507745465655
1117	1	0.9353370920351693
1117	2	0.3223754331055986
1117	3	0.0812223991507608
1117	4	-0.013753992602481446
1117	5	0.13584948409595654
1117	6	1.1005876880618217
1117	7	0.3903571627780664
1117	8	0.23696849256025132
1117	9	0.1692307743206385
1117	10-14	0.2288802885842145
1117	15-19	0.2137576116407871
1117	20-24	0.17176825684408215
1117	25-29	0.2766798941547677
1117	30-34	0.32434440118947094
1117	35-39	0.19211501066670422
1117	40-44	0.3009413689064715
1117	45-49	0.28665515752973647
1117	50-54	0.2665606869899122
1117	55-59	0.4380153577874353
1117	60-64	0.4463296197147244
1117	65-69	0.3800911173731194
1117	70-74	0.3831209813766563
1117	75-79	0.3881373955000669
1117	80-84	0.4629115572330562
1117	85-89	0.4363101515493213
1117	90-94	0.3910272369199106
1117	95-99	-0.2363180993788916
1117	100-104	0.4603751326758143
1117	105-109	0.6886048725869571
1117	110-114	0.6783071896516404
1117	115-119	1.0854666604615133
1117	120-124	0.7286294754321361
1117	125-129	0.6000136597486048
1117	130-134	0.7237936938151961
1117	135-139	0.5555108132099562
1117	140-144	0.71996057473185
1117	145-149	-0.43534999202934443
1117	150-151	0.29559896884308756
2117	1	-30.880956186987234
2117	2	-10.3513569890336
2117	3	-1.8602862152156021
2117	4	-0.22505164406119604
2117	5	0.07850502946926952
2117	6	1.2318998571522215
2117	7	-0.04533715915137293
2117	8	-0.06091178079798709
2117	9	0.06697749308820278
2117	10-14	0.3886148559797462
2117	15-19	0.3475264612232607
2117	20-24	0.21044852410567927
2117	25-29	0.40619965013662096
2117	30-34	0.6302380261963805
2117	35-39	0.3220526534377086
2117	40-44	0.18825448297657488
2117	45-49	0.3985212066572501
2117	50-54	0.3375708436164899
2117	55-59	0.5086807873435788
2117	60-64	0.5911346890735771
2117	65-69	0.5264322740968623
2117	70-74	0.4567452262026137
2117	75-79	0.5330025167946957
2117	80-84	0.5936009161138571
2117	85-89	0.34688519836964105
2117	90-94	0.5210918041820776
2117	95-99	0.12501535080677684
2117	100-104	0.9775214845016649
2117	105-109	0.8112115686064243
2117	110-114	0.836921689374428
2117	115-119	1.2115390695310353
2117	120-124	1.036551987096729
2117	125-129	0.8320635226693938
2117	130-134	1.0787494836257991
2117	135-139	0.6163760030918155
2117	140-144	0.9757920711338812
2117	145-149	1.2660251820583923
2117	150-151	1.7248939111059656
1116	1	0.32207029472777293
1116	2	0.21744250419349953
1116	3	-0.08369705476071232
1116	4	-0.2407591266290865
1116	5	0.09955824258953072
1116	6	1.2450783903970049
1116	7	-0.0695040293057616
1116	8	0.18652319258739425
1116	9	0.13855365981994794
1116	10-14	0.2678799880934051
1116	15-19	0.23911146738294775
1116	20-24	0.0550599923197197
1116	25-29	0.09313275005872157
1116	30-34	0.3188765301625196
1116	35-39	0.22058667678118127
1116	40-44	0.14411266835369219
1116	45-49	0.26826857910977964
1116	50-54	0.2599461567614867
1116	55-59	0.45862508872403396
1116	60-64	0.5447665025207513
1116	65-69	0.3595505592478432
1116	70-74	0.3059303905912003
1116	75-79	0.3036696537440946
1116	80-84	0.38278358753211705
1116	85-89	0.2780122136353498
1116	90-94	0.4738395568715603
1116	95-99	-0.24788122513385957
1116	100-104	0.7486216275918665
1116	105-109	0.4618602324097125
1116	110-114	0.5543378003328385
1116	115-119	0.9129729206865491
1116	120-124	0.6725498102696363
1116	125-129	0.5448921749987292
1116	130-134	0.6342211013158234
1116	135-139	0.5801881794037982
1116	140-144	0.5822192553632064
1116	145-149	0.6808529685327329
1116	150-151	0.8251176099740292
2118	1	0.7874691376880918
2118	2	0.39436854945971334
2118	3	0.07286035640735378
2118	4	-0.030482210921839226
2118	5	0.05056898048551517
2118	6	1.2422289515844511
2118	7	0.4552072566760188
2118	8	0.08878776544622724
2118	9	-0.048980800255499446
2118	10-14	0.21189345413556993
2118	15-19	0.11635580774204612
2118	20-24	0.1030729424843102
2118	25-29	0.1939460419580783
2118	30-34	0.29852348839553855
2118	35-39	0.08720639585992984
2118	40-44	0.19056061930984214
2118	45-49	0.2385149652373144
2118	50-54	0.05555068884142145
2118	55-59	0.22815508059008494
2118	60-64	0.3097970662193461
2118	65-69	0.22647373170379836
2118	70-74	0.23489456166602452
2118	75-79	0.20022374855638958
2118	80-84	0.23964718158833165
2118	85-89	0.15030385641880173
2118	90-94	0.29442621065493313
2118	95-99	0.31020754402048567
2118	100-104	0.6849209839731998
2118	105-109	0.3171102419630003
2118	110-114	0.5167657753632753
2118	115-119	0.850341187665471
2118	120-124	0.5111553304325298
2118	125-129	0.43236481165169494
2118	130-134	0.4628104764215877
2118	135-139	0.5123633471322506
2118	140-144	0.431092342658431
2118	145-149	0.42811857860571223
2118	150-151	1.295929926073228
1119	1	0.7828155251715785
1119	2	0.31082004151604536
1119	3	0.0309214485410223
1119	4	-0.03663432493685548
1119	5	0.14098931284132732
1119	6	1.3720631630718927
1119	7	0.26770054295963064
1119	8	0.35999673121164477
1119	9	0.26273839452821335
1119	10-14	0.3320259426148269
1119	15-19	0.23481852781765866
1119	20-24	-0.1406104574490925
1119	25-29	0.2846607707776414
1119	30-34	0.3745898279699418
1119	35-39	0.24104932712221228
1119	40-44	0.07113676359566057
1119	45-49	0.24589810628980757
1119	50-54	0.23222374696609194
1119	55-59	0.46801911440574173
1119	60-64	0.44888452446003413
1119	65-69	0.345479859984124
1119	70-74	0.280763947656375
1119	75-79	0.491984071566705
1119	80-84	0.3299259216654633
1119	85-89	0.3883743880377182
1119	90-94	0.49255845475491356
1119	95-99	0.4278648866778241
1119	100-104	0.752774904246472
1119	105-109	0.5419833527232072
1119	110-114	0.6037188088888925
1119	115-119	0.9477733925573588
1119	120-124	0.7541088822570501
1119	125-129	0.6067506408762284
1119	130-134	0.6688317401041459
1119	135-139	0.6197819366072892
1119	140-144	0.7232570999441492
1119	145-149	0.9370191538369355
1119	150-151	1.2957748392245918
2119	1	0.9144798358326511
2119	2	0.24235229452474982
2119	3	0.07305451330994828
2119	4	0.03180510954091176
2119	5	0.029860674936131204
2119	6	1.1728957532017432
2119	7	0.37796059339634525
2119	8	0.21293142435455792
2119	9	0.07608005982981325
2119	10-14	0.1512861366364433
2119	15-19	0.11373035658358077
2119	20-24	0.14732133366118205
2119	25-29	0.047125253740588846
2119	30-34	0.19337558515778142
2119	35-39	0.06910925257940903
2119	40-44	-0.23295439460859768
2119	45-49	0.08191991501450246
2119	50-54	0.06650312271561631
2119	55-59	0.28355985307921117
2119	60-64	0.29747587579607426
2119	65-69	0.12298314120221221
2119	70-74	0.11497962025047315
2119	75-79	0.03778057335342311
2119	80-84	0.14870904983418143
2119	85-89	0.27600586427239193
2119	90-94	0.17301695568821884
2119	95-99	0.25681604961431503
2119	100-104	0.6544038849353129
2119	105-109	0.32748754803102287
2119	110-114	0.2472937895251519
2119	115-119	0.7590041577434761
2119	120-124	0.48355369409006244
2119	125-129	0.38450549900019837
2119	130-134	0.4865693275948715
2119	135-139	0.2869477124409414
2119	140-144	0.2138015293559974
2119	145-149	0.5074922357147003
2119	150-151	0.4727331045573351
1118	1	0.8575175808982962
1118	2	0.45829900493428255
1118	3	0.06798054821872057
1118	4	0.04509964958648993
1118	5	0.09725202716855819
1118	6	1.204688044558253
1118	7	0.37870853679494587
1118	8	0.6166206737938396
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2111	1	0.9505911769218969
2111	2	0.35710864637522377
2111	3	0.08063645568313405
2111	4	0.06719263084590921
2111	5	0.027929382620882848
2111	6	0.9977874335011663
2111	7	0.3258976196777823
2111	8	-0.015243155946997433
2111	9	0.0011184254223834955
2111	10-14	-0.0756023360793634
2111	15-19	-0.07049435452603348
2111	20-24	-0.04688388112500519
2111	25-29	-0.048268534614081204
2111	30-34	-0.005399231083863754
2111	35-39	-0.17241473740029534
2111	40-44	-0.16043233330120188
2111	45-49	-0.20167522658972814
2111	50-54	-0.28073209181555114
2111	55-59	-0.2402206660379136
2111	60-64	-0.32581983777249945
2111	65-69	-0.42309909688209757
2111	70-74	-0.2908206495051502
2111	75-79	-0.5060024539808978
2111	80-84	-0.22232645611130408
2111	85-89	-0.31764611045959157
2111	90-94	-0.6194580084264558
2111	95-99	-0.35957287915685754
2111	100-104	-0.546620117445741
2111	105-109	-0.4408394326658396
2111	110-114	-0.5763176869309703
2111	115-119	-1.75643465276071
2111	120-124	-0.8895222710058874
2111	125-129	-0.8417168289537429
2111	130-134	-0.5952498355339202
2111	135-139	-0.4438760762176486
2111	140-144	-1.060727070782832
2111	145-149	-1.644930412159205
2111	150-151	-2.633104578856045
2110	1	0.9376220947397584
2110	2	0.3179974581654861
2110	3	0.06197831705551238
2110	4	0.03549587172381763
2110	5	0.11071250028886936
2110	6	1.1267223065722476
2110	7	0.15475691656693158
2110	8	-0.0420931694714497
2110	9	-0.055749707318781816
2110	10-14	0.0484180476188385
2110	15-19	-0.08176580461855565
2110	20-24	-0.21585730683462856
2110	25-29	-0.23836074578100153
2110	30-34	0.06146133282324229
2110	35-39	-0.2488327067731575
2110	40-44	-0.2680917727812826
2110	45-49	-0.13937458143495007
2110	50-54	-0.29640684991288424
2110	55-59	-0.4821228828333233
2110	60-64	-0.13165040094497016
2110	65-69	-0.23829602209234224
2110	70-74	-0.1990254787166208
2110	75-79	-0.2934386754783773
2110	80-84	-0.30655133478844476
2110	85-89	-0.30823795066204696
2110	90-94	-0.4214242602871252
2110	95-99	-0.5528926026710295
2110	100-104	-0.0055025806871711325
2110	105-109	-0.2202787482443469
2110	110-114	-0.6294621925911414
2110	115-119	-6.923916257353525
2110	120-124	-3.0103965694774146
2110	125-129	-0.4786772644579784
2110	130-134	-0.8290507641694802
2110	135-139	-0.8431475315442398
2110	140-144	-0.8054280737982111
2110	145-149	-0.8682143813911125
2110	150-151	-1.4196991624570856
2109	1	0.9420943699964823
2109	2	0.38210523971889643
2109	3	0.06737363554466924
2109	4	0.06124448033891383
2109	5	0.09099898749649782
2109	6	1.044342192884713
2109	7	0.37366353261943885
2109	8	0.006281769942162896
2109	9	-0.19307182063186445
2109	10-14	-0.061411876994313275
2109	15-19	-0.05834733829786387
2109	20-24	-0.39649339199526423
2109	25-29	-0.22416740522027112
2109	30-34	-0.21862664403415266
2109	35-39	-0.4041968024841509
2109	40-44	-0.22999463948449517
2109	45-49	-0.39314539989027963
2109	50-54	-0.3046016053082994
2109	55-59	-0.719864177358474
2109	60-64	-0.57780816809219
2109	65-69	-0.555255839068181
2109	70-74	-0.7224860985906005
2109	75-79	-0.6220822391576988
2109	80-84	-0.5318645061669329
2109	85-89	-0.5743124869636205
2109	90-94	-0.693747761902074
2109	95-99	-0.45847088551913373
2109	100-104	-0.6411957656011396
2109	105-109	-0.6615286534175482
2109	110-114	-1.4821610850513807
2109	115-119	-5.41839657093411
2109	120-124	-1.850090343345272
2109	125-129	-1.096601680296807
2109	130-134	-1.174912372708711
2109	135-139	-1.7231163349251943
2109	140-144	-0.9384070257966002
2109	145-149	-1.1227904149286019
2109	150-151	-2.335657477525743
2108	1	0.7329051991513822
2108	2	0.1389699127915307
2108	3	0.0642449976910271
2108	4	-0.024753654043593087
2108	5	-0.003673285846382157
2108	6	0.7675911447586543
2108	7	-0.17751837490969535
2108	8	-0.4300525819343761
2108	9	-0.27122435919785914
2108	10-14	-0.30837984669076235
2108	15-19	-0.40950959834744083
2108	20-24	-0.6356906942177147
2108	25-29	-0.3706983971098907
2108	30-34	-7.297357711566558
2108	35-39	-1.181561834346553
2108	40-44	-0.7639826357675545
2108	45-49	-0.387900773141034
2108	50-54	-0.42500618263520096
2108	55-59	-0.5279676463442584
2108	60-64	-0.760173215583265
2108	65-69	-0.7049707943277426
2108	70-74	-0.5613972620652206
2108	75-79	-0.9024013588791746
2108	80-84	-0.5192577455585194
2108	85-89	-0.7658172293739938
2108	90-94	-0.9851662786691833
2108	95-99	-0.8915322352205948
2108	100-104	-0.7760213696606613
2108	105-109	-0.994722980832961
2108	110-114	-1.3198636159593136
2108	115-119	-1.109267146288218
2108	120-124	-1.1579565243619712
2108	125-129	-1.1059614269872071
2108	130-134	-1.615990965752701
2108	135-139	-2.0953819527736783
2108	140-144	-2.0330688055992567
2108	145-149	-2.0442779702596994
2108	150-151	-2.281877525724582
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	7.0
19	18.0
20	71.0
21	247.0
22	600.0
23	1069.0
24	1625.0
25	2424.0
26	3497.0
27	5053.0
28	6801.0
29	9407.0
30	12942.0
31	17813.0
32	23870.0
33	33126.0
34	47059.0
35	73767.0
36	143762.0
37	517255.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.10306756841308937	0.1457279858026131	99.57089308161791	0.18031136416638702
2	30.019602072377737	26.783316581798395	24.84298906615283	18.354092279671043
3	0.05475253077747483	0.1156133560635929	0.13016220298417952	99.69947191017475
4	0.2687649583803024	27.302521615033072	0.17036588684106774	72.25834753974556
5	47.75053725226701	24.936279382284834	0.12727464558009363	27.185908719868063
6	51.921058622968296	30.226617726270668	0.4068124142756395	17.445511236485398
7	0.5674050299028781	42.378792001465996	0.28464652410277486	56.769156444528356
8	47.54818611418069	27.333507327176914	24.740147598607308	0.3781589600350949
9	29.965626960901364	17.90640982213757	27.460226673256223	24.667736543704848
10-14	22.95248302171776	49.331208387243656	22.498159182154893	5.218149408883681
15-19	30.937156755496076	26.62383456517273	15.597407861930332	26.841600817400863
20-24	28.600323184309456	24.039181932775442	16.44781572941366	30.912679153501443
25-29	27.950045256909313	39.23897314016315	6.218377081679003	26.592604521248536
30-34	23.82126019668708	16.518183282153228	29.48500413698128	30.175552384178406
35-39	37.26892599523553	15.887251989360463	21.144405635179332	25.699416380224676
40-44	9.138297340670691	29.816895542610904	33.9857954387699	27.0590116779485
45-49	5.3233897702725965	28.841967315071383	25.81078724810226	40.02385566655376
50-54	26.37206177151647	20.8950539473465	15.612245464591327	37.12063881654571
55-59	15.216961067952	27.7409194649134	41.096649878111755	15.94546958902284
60-64	27.006857948834707	14.858681830045034	32.278227261873695	25.856232959246572
65-69	19.30658640737882	30.2440985545554	25.096205638511133	25.35310939955465
70-74	20.210236390997487	15.00274873252889	39.73592176940633	25.051093107067295
75-79	34.88355924768023	27.89438203495055	22.240100398149742	14.981958319219471
80-84	11.155700427025316	20.66547092174164	42.333679470022155	25.845149181210886
85-89	24.769578472148954	30.41228766735339	32.5368191333996	12.28131472709806
90-94	20.688193777313792	35.20903139108078	19.41857921069729	24.684195620908138
95-99	6.3622662883226075	25.695396011838984	41.837996923640766	26.104340776197642
100-104	19.343791473931464	35.30516484065681	14.9014843155656	30.449559369846124
105-109	21.081012644169633	38.72603188529734	24.635262628898897	15.557692841634136
110-114	35.699027670574125	27.247857932175716	16.267054635917884	20.78605976133227
115-119	20.273318414286745	40.60709783821904	10.363310251384084	28.75627349611013
120-124	0.4444395084488819	20.32902606020557	47.52577422632897	31.700760205016575
125-129	24.91864307013988	34.0690459399277	8.81255865350977	32.19975233642265
130-134	14.85112975683435	31.295702537163418	37.98437387204789	15.868793833954342
135-139	36.52642392674489	16.21216883326022	27.66391053014443	19.597496709850457
140-144	23.9568199108189	38.278704819444364	13.016264722378015	24.748210547358717
145-149	32.53446466351627	22.518949595464314	28.245153623606896	16.701432117412526
150-151	40.75481861141807	18.06150497270703	25.70248163346901	15.481194782405892
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	0.5
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.5
22	0.5
23	0.5
24	0.5
25	0.0
26	1.0
27	3.0
28	5.5
29	4.5
30	3.5
31	6.0
32	25.0
33	100.0
34	550.0
35	5045.0
36	121287.0
37	128704.0
38	12967.5
39	1193.0
40	357.5
41	280.5
42	323.5
43	384.5
44	523.0
45	1196.5
46	7624.5
47	70762.5
48	73392.0
49	18665.0
50	99624.0
51	101018.5
52	11815.5
53	1411.5
54	460.0
55	552.5
56	1504.0
57	11209.0
58	111422.0
59	108632.0
60	8109.5
61	962.0
62	168.5
63	59.5
64	34.0
65	10.5
66	3.0
67	1.5
68	3.5
69	3.0
70	0.5
71	0.0
72	0.5
73	0.5
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.374460665359862
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	900415.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	1.149520433131408
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.78202896768913	0.8251490902998928
2	9.156515356734733	0.210512029976962
3	3.9531827664733834	0.13632793097892304
4	2.314983887504683	0.10644485124226455
5	1.3888632736701099	0.07982633559547851
6	0.8241423347485994	0.056842107216128396
7	0.6686680491762994	0.05380533098571913
8	0.639497556532855	0.05880924065376997
9	0.39724537828163275	0.04109775113715772
>10	5.018761534986018	1.2305698891983927
>50	1.462996846795433	1.2424864001949396
>100	1.9668103304600857	4.2024685674574815
>500	0.18408569595439225	1.5036580216055826
>1k	0.1743969751146874	5.780635678497486
>5k	0.029066162519114567	1.8400069754052168
>10k+	0.03875488335881942	82.6313597995546
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	232563	25.82842356024722	No Hit
TACACGCATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC	204265	22.685650505600197	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	179134	19.894604154750866	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	125966	13.989771383195526	No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	6038	0.6705796771488702	No Hit
NACACGCATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC	5451	0.6053875157566234	No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG	5032	0.5588534175907777	No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC	4739	0.5263128668447327	No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	4698	0.5217594109382896	No Hit
TACACGCATGGTAACAGCATTCGTCGACGAAGCCTGGCCCATTGTCAACC	4194	0.4657852212590861	No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	4129	0.45856632774887135	No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC	3903	0.43346679031335555	No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG	3637	0.4039248568715537	No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	3499	0.388598590649867	No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	3246	0.3605004359101081	No Hit
TTCCAGATCTCGATGCCGACTGGCAGAGAATTTTCCGGCACATATTCGCC	3054	0.33917693507993535	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG	3009	0.3341792395728636	No Hit
TCCACGCATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC	2836	0.31496587684567673	No Hit
TACACACATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC	2392	0.26565528117590226	No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC	1878	0.20857049249512727	No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	1708	0.18969030946841178	No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	1543	0.17136542594248208	No Hit
TACACGCATGCTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC	1298	0.14415575040398038	No Hit
TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	1127	0.12516450747710778	No Hit
TACACGCATGATAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC	1013	0.1125036788591927	No Hit
TACACGCATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACA	973	0.10806128285290671	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	2.221198003142995E-4
7	0.0	0.0	2.221198003142995E-4
8	0.0	0.0	2.221198003142995E-4
9	0.0	0.0	2.221198003142995E-4
10-11	0.0	0.0	0.008440552411943381
12-13	0.0	0.0	0.008440552411943381
14-15	0.0	0.0	0.008440552411943381
16-17	0.0	0.0	0.008440552411943381
18-19	0.0	0.0	0.008440552411943381
20-21	0.0	0.0	0.008607142262179106
22-23	0.0	0.0	0.009329031613200579
24-25	0.0	0.0	0.010106450914300628
26-27	0.0	0.0	0.010273040764536352
28-29	0.0	0.0	0.010661750415086376
30-31	0.0	0.0	0.011272579865950701
32-33	0.0	0.0	0.011772349416657875
34-35	0.0	0.0	0.011883409316815025
36-37	0.0	0.0	0.016436865223258164
38-39	0.0	0.0	0.020768201329387004
40-41	0.0	0.0	0.026765435937873092
42-43	0.0	0.0	0.07474331280576178
44-45	0.0	0.0	0.08101819716464076
46-47	0.0	0.0	0.11017142095589255
48-49	0.0	0.0	0.22894998417396423
50-51	0.0	0.0	0.3330131106212135
52-53	0.0	0.0	0.33362394007207785
54-55	0.0	0.0	0.3339571197725493
56-57	0.0	0.0	0.3346234791734922
58-59	0.0	0.0	0.3357896081251423
60-61	0.0	0.0	0.356724399304765
62-63	0.0	0.0	0.3587790074576723
64-65	0.0	0.0	0.3598340765091652
66-67	0.0	0.0	0.3598896064592438
68-69	0.0	0.0	0.36172209481183676
70-71	0.0	0.0	0.3618331547119939
72-73	0.0	0.0	0.36194421461215104
74-75	0.0	0.0	0.36194421461215104
76-77	0.0	0.0	0.36261057401309393
78-79	0.0	0.0	0.3632769334140369
80-81	0.0	0.0	0.36333246336411545
82-83	0.0	0.0	0.363387993314194
84-85	0.0	0.0	0.3641098826652155
86-87	0.0	0.0	0.365220481666787
88-89	0.0	0.0	0.3796027387371379
90-91	0.0	0.0	0.38004697833776646
92-93	0.0	0.0	0.38004697833776646
94-95	0.0	0.0	0.3801580382379236
96-97	0.0	0.0	0.3802690981380808
98-99	0.0	0.0	0.3802690981380808
100-101	0.0	0.0	0.38121310728941654
102-103	0.0	0.0	0.424915178001255
104-105	0.0	0.0	0.464230382656886
106-107	0.0	0.0	0.4643969725071217
108-109	0.0	0.0	0.46461909230743603
110-111	0.0	0.0	0.47489213307197237
112-113	0.0	0.0	0.48472093423588014
114-115	0.0	0.0	0.48488752408611585
116-117	0.0	0.0	0.48488752408611585
118-119	0.0	0.0	0.48633130278815884
120-121	0.0	0.0	0.4873863718396517
122-123	0.0	0.0	0.4929948967975878
124-125	0.0	0.0	0.5257020373938683
126-127	0.0	0.0	0.5269792262456756
128-129	0.0	0.0	0.5270347561957542
130-131	0.0	0.0	0.5270902861458328
132-133	0.0	0.0	0.5272568759960685
134-135	0.0	0.0	0.527312405946147
136-137	0.0	0.0	0.5273679358962255
138-139	0.0	0.0	0.5275345257464613
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCCGAA	180	0.0	148.50581	1
TGCACGC	25	3.2177923E-8	148.5058	1
TTCCGCA	35	9.094947E-12	148.5058	1
TGCCGCG	20	1.8916126E-6	148.5058	1
TGCCGAG	70	0.0	148.5058	1
TGCGAGG	10	0.0066019148	148.5058	1
ACCCGCA	20	1.8916126E-6	148.5058	1
TACACTC	105	0.0	148.5058	1
TACACGC	23160	0.0	148.5058	1
TACGCGC	15	1.1182969E-4	148.5058	1
TTCCATA	110	0.0	148.5058	1
TGCCAGA	10	0.0066019148	148.5058	1
TGGCGAC	15	1.1182969E-4	148.5058	1
TCCCGCG	40	0.0	148.5058	1
TGCAGAC	15	1.1182969E-4	148.5058	1
AGTTTGG	35	9.094947E-12	148.5058	1
TCCCGCA	14385	0.0	148.35095	1
TTCCAGA	21240	0.0	148.2611	1
ACACGTA	25	3.71474E-8	144.97624	2
ACAGATG	50	0.0	144.97624	5
>>END_MODULE