FastQCFastQC Report
Wed 17 Jun 2015
000000000-AG1W8 l01n01 mis24.3410000000d2f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG1W8 l01n01 mis24.3410000000d2f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences900415
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG23256325.82842356024722No Hit
TACACGCATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC20426522.685650505600197No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC17913419.894604154750866No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG12596613.989771383195526No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG60380.6705796771488702No Hit
NACACGCATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC54510.6053875157566234No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG50320.5588534175907777No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC47390.5263128668447327No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC46980.5217594109382896No Hit
TACACGCATGGTAACAGCATTCGTCGACGAAGCCTGGCCCATTGTCAACC41940.4657852212590861No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG41290.45856632774887135No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC39030.43346679031335555No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG36370.4039248568715537No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG34990.388598590649867No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG32460.3605004359101081No Hit
TTCCAGATCTCGATGCCGACTGGCAGAGAATTTTCCGGCACATATTCGCC30540.33917693507993535No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG30090.3341792395728636No Hit
TCCACGCATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC28360.31496587684567673No Hit
TACACACATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC23920.26565528117590226No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC18780.20857049249512727No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG17080.18969030946841178No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC15430.17136542594248208No Hit
TACACGCATGCTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC12980.14415575040398038No Hit
TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG11270.12516450747710778No Hit
TACACGCATGATAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC10130.1125036788591927No Hit
TACACGCATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACA9730.10806128285290671No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCGAA1800.0148.505811
TGCACGC253.2177923E-8148.50581
TTCCGCA359.094947E-12148.50581
TGCCGCG201.8916126E-6148.50581
TGCCGAG700.0148.50581
TGCGAGG100.0066019148148.50581
ACCCGCA201.8916126E-6148.50581
TACACTC1050.0148.50581
TACACGC231600.0148.50581
TACGCGC151.1182969E-4148.50581
TTCCATA1100.0148.50581
TGCCAGA100.0066019148148.50581
TGGCGAC151.1182969E-4148.50581
TCCCGCG400.0148.50581
TGCAGAC151.1182969E-4148.50581
AGTTTGG359.094947E-12148.50581
TCCCGCA143850.0148.350951
TTCCAGA212400.0148.26111
ACACGTA253.71474E-8144.976242
ACAGATG500.0144.976245
Produced by FastQC (version 0.11.2)