##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AG1W8 l01n01 mis23.3410000000d2e9.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1063320
Sequences flagged as poor quality	0
Sequence length	151
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.98824624760185	34.0	34.0	34.0	34.0	34.0
2	33.254781251175565	34.0	34.0	34.0	34.0	34.0
3	33.87932325170222	34.0	34.0	34.0	34.0	34.0
4	33.82315295489599	34.0	34.0	34.0	34.0	34.0
5	33.78569010269721	34.0	34.0	34.0	34.0	34.0
6	36.356033931459955	38.0	38.0	38.0	34.0	38.0
7	37.19144001805665	38.0	38.0	38.0	36.0	38.0
8	36.693810894180494	38.0	37.0	38.0	34.0	38.0
9	37.18098126622277	38.0	38.0	38.0	36.0	38.0
10-14	37.163417221532555	38.0	38.0	38.0	35.8	38.0
15-19	37.354320242260094	38.0	38.0	38.0	36.8	38.0
20-24	37.455811232742725	38.0	38.0	38.0	36.8	38.0
25-29	37.20960632735206	38.0	38.0	38.0	36.0	38.0
30-34	36.888891961027724	38.0	38.0	38.0	35.2	38.0
35-39	37.16994432532069	38.0	38.0	38.0	36.2	38.0
40-44	37.207314637174136	38.0	38.0	38.0	36.6	38.0
45-49	37.13385772862355	38.0	38.0	38.0	36.4	38.0
50-54	36.959768837226804	38.0	37.8	38.0	35.6	38.0
55-59	36.46023812210811	38.0	37.2	38.0	33.4	38.0
60-64	36.670624271150736	38.0	37.2	38.0	34.2	38.0
65-69	36.668022044163564	38.0	37.2	38.0	34.2	38.0
70-74	36.762527743294584	38.0	37.8	38.0	34.8	38.0
75-79	36.39715494865139	38.0	37.2	38.0	33.6	38.0
80-84	36.68263307376895	38.0	37.0	38.0	34.4	38.0
85-89	36.61352687807998	38.0	37.0	38.0	34.0	38.0
90-94	36.397356393183614	38.0	37.0	38.0	34.0	38.0
95-99	36.390414738742805	38.0	37.0	38.0	33.4	38.0
100-104	35.832394763570704	38.0	36.6	38.0	31.2	38.0
105-109	35.74807019523755	38.0	36.6	38.0	30.8	38.0
110-114	36.132078207877214	38.0	37.0	38.0	33.0	38.0
115-119	35.56050803144867	38.0	36.4	38.0	30.0	38.0
120-124	35.884263062859716	38.0	36.6	38.0	31.6	38.0
125-129	35.553688447504044	38.0	36.8	38.0	30.0	38.0
130-134	35.43955911672874	38.0	36.0	38.0	29.8	38.0
135-139	34.66371835383516	37.8	35.0	38.0	26.2	38.0
140-144	35.48431422337585	38.0	36.4	38.0	29.6	38.0
145-149	34.958303615092355	37.8	35.2	38.0	27.2	38.0
150-151	33.10895967347553	36.0	31.5	37.5	23.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.6656827236828491
1101	2	0.6162724230362286
1101	3	0.06814809506742137
1101	4	0.02823678938040075
1101	5	0.1349738366529607
1101	6	1.1357514759549403
1101	7	0.33806174073237116
1101	8	0.4901666497527444
1101	9	0.30524167073676267
1101	10-14	0.3175164214130106
1101	15-19	0.26407182096473036
1101	20-24	0.1852421709877703
1101	25-29	0.23023958451378235
1101	30-34	0.2671331605112144
1101	35-39	0.1880576216146821
1101	40-44	0.21511053726607088
1101	45-49	0.20701776297296703
1101	50-54	0.19661928679045104
1101	55-59	0.3279223943571452
1101	60-64	0.3212284128339107
1101	65-69	0.2616060603971704
1101	70-74	0.21476017074754594
1101	75-79	0.27968441830297763
1101	80-84	0.2997606565836435
1101	85-89	0.3774677421350674
1101	90-94	0.31779621382558076
1101	95-99	0.366972453735265
1101	100-104	0.6153360737439826
1101	105-109	0.5277621420294452
1101	110-114	0.24680262932205466
1101	115-119	0.680016478521118
1101	120-124	0.381565953939365
1101	125-129	0.3385942619920925
1101	130-134	0.5092850749438256
1101	135-139	0.5637671068890384
1101	140-144	0.4939383828025541
1101	145-149	0.5653330214743377
1101	150-151	0.33493946412638564
1102	1	0.8365786011346614
1102	2	0.3213802119090232
1102	3	0.06434081543820014
1102	4	0.005349405588908951
1102	5	0.1133830646739753
1102	6	0.9631100972654707
1102	7	0.40094411389682705
1102	8	0.21054539538011596
1102	9	0.14888283762015675
1102	10-14	0.23980221866366236
1102	15-19	0.20411025486485812
1102	20-24	0.12788147395276894
1102	25-29	0.20153262637903424
1102	30-34	0.26102066869674445
1102	35-39	0.1990853343418877
1102	40-44	0.21168241666586596
1102	45-49	0.17141626172734448
1102	50-54	0.11911987856493056
1102	55-59	0.252951634429067
1102	60-64	0.3997729800827159
1102	65-69	0.28705596153566404
1102	70-74	0.2783566578796126
1102	75-79	0.25640898485697505
1102	80-84	0.3601932266057304
1102	85-89	0.31846053612121494
1102	90-94	0.3335454214828246
1102	95-99	0.31302179720490386
1102	100-104	0.7145718659578648
1102	105-109	0.41256951990380486
1102	110-114	0.19096093425581273
1102	115-119	0.6295715425603987
1102	120-124	0.4636382068325844
1102	125-129	0.4914645581009722
1102	130-134	0.6239467001028487
1102	135-139	0.7999018962428508
1102	140-144	0.37062010251848676
1102	145-149	0.7469183943283824
1102	150-151	0.9976799981939735
1103	1	0.800183169495277
1103	2	0.2609649251951396
1103	3	0.07837184305349609
1103	4	0.01064103991821952
1103	5	0.11415029996118164
1103	6	1.1529140645881881
1103	7	0.39953086994382403
1103	8	0.2309940495676983
1103	9	0.10930228419170618
1103	10-14	0.15151851932439087
1103	15-19	0.22212394005815383
1103	20-24	0.1153673667431434
1103	25-29	0.15645200267607606
1103	30-34	0.30623453091783404
1103	35-39	0.1380001382415159
1103	40-44	0.16995618896017106
1103	45-49	0.12073701112201007
1103	50-54	0.1215099506338504
1103	55-59	0.33933501724992965
1103	60-64	0.3843420249818621
1103	65-69	0.2773296481713672
1103	70-74	0.20158028173592157
1103	75-79	0.2053693773506211
1103	80-84	0.27772710052364147
1103	85-89	0.27371297179172416
1103	90-94	0.2687201441200031
1103	95-99	0.23655667195011176
1103	100-104	0.5272854001901663
1103	105-109	0.29778270276352714
1103	110-114	0.2784782352023214
1103	115-119	0.8103702549244503
1103	120-124	0.5591104355981713
1103	125-129	0.45360660419466114
1103	130-134	0.6190864848585207
1103	135-139	0.5804962340612008
1103	140-144	0.6258700521202059
1103	145-149	0.8532065976366212
1103	150-151	0.776762695426001
2116	1	0.7440172757154073
2116	2	0.28801469647029876
2116	3	-0.015700462266934778
2116	4	-0.13399873302380172
2116	5	0.027468930459484397
2116	6	0.9832103150612639
2116	7	-0.024697091889599676
2116	8	0.15547660046398448
2116	9	0.11262055497212486
2116	10-14	0.16640866097723972
2116	15-19	0.1763794785735513
2116	20-24	0.09428192057391271
2116	25-29	0.1683379933872402
2116	30-34	0.24798804918668793
2116	35-39	0.11511179619136414
2116	40-44	0.0026903133688520597
2116	45-49	0.15911657833580506
2116	50-54	0.12993170616856276
2116	55-59	0.3099928297903034
2116	60-64	0.3403261345711712
2116	65-69	0.36378029158505854
2116	70-74	0.23862271172647098
2116	75-79	0.3180017573302365
2116	80-84	0.35009990605458086
2116	85-89	0.27922835885069475
2116	90-94	0.3508513864285021
2116	95-99	0.38414734745469303
2116	100-104	0.6396487517272504
2116	105-109	0.39733310607819305
2116	110-114	0.2982208697254052
2116	115-119	0.7724764081774396
2116	120-124	0.6014033786933339
2116	125-129	0.5747242729796085
2116	130-134	0.6864200988731284
2116	135-139	0.742970920815317
2116	140-144	0.729317618679417
2116	145-149	0.8323068874881656
2116	150-151	1.5622502575469994
1117	1	0.8806839347797251
1117	2	0.33153396044132677
1117	3	0.0596981098599727
1117	4	0.05352421656578343
1117	5	0.11587284019527999
1117	6	1.169636625877203
1117	7	0.39304929832525914
1117	8	0.26686507059017117
1117	9	0.15516101657152603
1117	10-14	0.2313867700146801
1117	15-19	0.20599252663636491
1117	20-24	0.16822411079532174
1117	25-29	0.22303407810174036
1117	30-34	0.27341813304818885
1117	35-39	0.19322835996023713
1117	40-44	0.2623781761434216
1117	45-49	0.26281846592291913
1117	50-54	0.24107500055507103
1117	55-59	0.46140409175735186
1117	60-64	0.41575770089064434
1117	65-69	0.3514417559646361
1117	70-74	0.2887926622275714
1117	75-79	0.2997039687774006
1117	80-84	0.30538159967537126
1117	85-89	0.4010224674867331
1117	90-94	0.4184823349811282
1117	95-99	-0.2622696276772558
1117	100-104	0.44356897876379264
1117	105-109	0.6012803214519664
1117	110-114	0.5232514501723173
1117	115-119	1.0009266770344993
1117	120-124	0.7205439636028288
1117	125-129	0.6414270508017381
1117	130-134	0.8290975547928099
1117	135-139	0.5431961784018924
1117	140-144	0.5636790176027731
1117	145-149	-0.7126530215269185
1117	150-151	0.11146044955951595
2117	1	-30.902491003622245
2117	2	-10.309858013824073
2117	3	-1.8830549341156981
2117	4	-0.32522950287039265
2117	5	0.08062000799996838
2117	6	1.2366511994410345
2117	7	0.015026116274391654
2117	8	-0.110268494177852
2117	9	0.09549347398689889
2117	10-14	0.3800518268710604
2117	15-19	0.3636219733598267
2117	20-24	0.23402788783855755
2117	25-29	0.3844643601484421
2117	30-34	0.5864691708557999
2117	35-39	0.2845030931798931
2117	40-44	0.15453142739823988
2117	45-49	0.3786027144409445
2117	50-54	0.3012221872033152
2117	55-59	0.48994263396483007
2117	60-64	0.5675139960791853
2117	65-69	0.4761419714354176
2117	70-74	0.4800555972544842
2117	75-79	0.5571096692056301
2117	80-84	0.5356794425351907
2117	85-89	0.33731656008689015
2117	90-94	0.4846456773252612
2117	95-99	0.05743531126429957
2117	100-104	0.9486410553771165
2117	105-109	0.7317390335086884
2117	110-114	0.7236839573805369
2117	115-119	1.1851667797626462
2117	120-124	0.9954804085277686
2117	125-129	0.8625486116075791
2117	130-134	1.0838321947060976
2117	135-139	0.6837907402178516
2117	140-144	1.0280733020214115
2117	145-149	1.2216237199017073
2117	150-151	1.591666395121763
1116	1	0.372238238255008
1116	2	0.08949824565640085
1116	3	-0.06883013247093572
1116	4	-0.2489836714128444
1116	5	0.0774532060856643
1116	6	1.1267889226354768
1116	7	-0.17188372851101974
1116	8	0.17521393844445043
1116	9	0.10767608410380092
1116	10-14	0.1907280554978783
1116	15-19	0.19840087931636674
1116	20-24	0.06531419322676157
1116	25-29	0.011577162412564235
1116	30-34	0.20289163668307708
1116	35-39	0.058954365320133206
1116	40-44	-0.03296716038703096
1116	45-49	0.17598818167612507
1116	50-54	0.05503705947315751
1116	55-59	0.2892531864346992
1116	60-64	0.33009629880641
1116	65-69	0.2469974724777373
1116	70-74	0.16420369485070552
1116	75-79	0.14801535046661485
1116	80-84	0.28125890936319564
1116	85-89	0.24072090081917707
1116	90-94	0.30379439142131304
1116	95-99	-0.5825470252987301
1116	100-104	0.5680213440137294
1116	105-109	0.43700468758633093
1116	110-114	0.4377745054345539
1116	115-119	0.8555317189872298
1116	120-124	0.5369988906191665
1116	125-129	0.4916744721983264
1116	130-134	0.5296945632697714
1116	135-139	0.44663306250102863
1116	140-144	0.4492272750043824
1116	145-149	0.6655831972245423
1116	150-151	0.5915984367684786
2118	1	0.8947874332235344
2118	2	0.4351871515643353
2118	3	0.06415335877839823
2118	4	-0.049005337101199586
2118	5	0.051188093638224075
2118	6	1.1744733392112394
2118	7	0.46126830163955646
2118	8	0.05833888648459151
2118	9	-0.04660553457504335
2118	10-14	0.22495299068975783
2118	15-19	0.137861655603011
2118	20-24	0.08271410908327681
2118	25-29	0.1693507358151649
2118	30-34	0.294913272140235
2118	35-39	0.10559222837785853
2118	40-44	0.17040951272055338
2118	45-49	0.1796058477776441
2118	50-54	0.0470426220126825
2118	55-59	0.22343907300221844
2118	60-64	0.2883034882879798
2118	65-69	0.24995352891937017
2118	70-74	0.28040479274685737
2118	75-79	0.2686946453744241
2118	80-84	0.28505635029270593
2118	85-89	0.1561726178249856
2118	90-94	0.2967783361385443
2118	95-99	0.33445353649759824
2118	100-104	0.6604746809076332
2118	105-109	0.39189239130048037
2118	110-114	0.4772162360695873
2118	115-119	0.7696518023245602
2118	120-124	0.42659694171492646
2118	125-129	0.4319913454868072
2118	130-134	0.5129473388711858
2118	135-139	0.6075258904023997
2118	140-144	0.452399534861641
2118	145-149	0.4120245575274595
2118	150-151	1.3048395733605602
1119	1	0.8163931165494276
1119	2	0.31618128434499226
1119	3	0.025655285067273326
1119	4	-0.03362547533076565
1119	5	0.11168674239075216
1119	6	1.3290244525053794
1119	7	0.3236901444276299
1119	8	0.22271087837410874
1119	9	0.1640570409116222
1119	10-14	0.2720700964744154
1119	15-19	0.14753527967169333
1119	20-24	-0.28955521794856764
1119	25-29	0.18096742080128791
1119	30-34	0.27131186568480103
1119	35-39	0.13083110517024465
1119	40-44	-0.06392799665132287
1119	45-49	0.11596483734202678
1119	50-54	0.10758939054952066
1119	55-59	0.30718876227021497
1119	60-64	0.28305054494452975
1119	65-69	0.2037298338191036
1119	70-74	0.14680101025228254
1119	75-79	0.3267607990961636
1119	80-84	0.1413379289011658
1119	85-89	0.1585372759399064
1119	90-94	0.27502260294576786
1119	95-99	0.20021411361555153
1119	100-104	0.5169977569398867
1119	105-109	0.2637661776985638
1119	110-114	0.35645833948318995
1119	115-119	0.7892173864957428
1119	120-124	0.5304630968343389
1119	125-129	0.3643014844850967
1119	130-134	0.4916568301492177
1119	135-139	0.2934450631414549
1119	140-144	0.5191828071467484
1119	145-149	0.7259068158029152
1119	150-151	1.077660655616441
2119	1	0.8279559237520573
2119	2	0.2820173550285645
2119	3	0.06445622575668608
2119	4	0.010192608763830435
2119	5	0.0748129362351122
2119	6	1.2307096212620223
2119	7	0.45578477682347085
2119	8	0.2121356152288314
2119	9	-0.016836853798302798
2119	10-14	0.14260601770266845
2119	15-19	0.15943036522887866
2119	20-24	0.1145111458113135
2119	25-29	0.13741817646980792
2119	30-34	0.16415719140949392
2119	35-39	0.11053005658527582
2119	40-44	-0.17961051597261957
2119	45-49	0.12626246923436923
2119	50-54	0.09027575015342393
2119	55-59	0.32691836401573227
2119	60-64	0.28939000834395756
2119	65-69	0.17168894939522517
2119	70-74	0.18462909266850858
2119	75-79	0.06203815061989815
2119	80-84	0.29278942385404605
2119	85-89	0.30880441734701947
2119	90-94	0.29768786297407246
2119	95-99	0.31059346640205376
2119	100-104	0.6460157675056593
2119	105-109	0.49051728262899275
2119	110-114	0.36764880711053394
2119	115-119	0.7844772749237521
2119	120-124	0.43553558666418724
2119	125-129	0.43595522366556594
2119	130-134	0.5578043993022774
2119	135-139	0.33298508839700247
2119	140-144	0.34544548487469484
2119	145-149	0.5194628535583661
2119	150-151	0.5429013026700886
1118	1	0.7616625261926941
1118	2	0.39907876542292087
1118	3	0.07938547438109822
1118	4	0.06884392921366356
1118	5	0.11383364568253995
1118	6	1.1543984568844934
1118	7	0.3695360164802324
1118	8	0.7158715769535959
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2111	2	0.2873273638164662
2111	3	0.07075683463246207
2111	4	0.049971577441247916
2111	5	0.0016634948943305972
2111	6	0.9141314874348936
2111	7	0.2912039281636254
2111	8	-0.006877237071712727
2111	9	0.012143593129074759
2111	10-14	-0.04849348954735433
2111	15-19	-0.05485075334497935
2111	20-24	-0.0285216989567445
2111	25-29	-0.06874394455210364
2111	30-34	4.017774367426341E-4
2111	35-39	-0.13827102307073602
2111	40-44	-0.04430827505868251
2111	45-49	-0.08611708948762242
2111	50-54	-0.26216485307298854
2111	55-59	-0.20082981563401603
2111	60-64	-0.23416626558736908
2111	65-69	-0.3668820756502811
2111	70-74	-0.28522527931065866
2111	75-79	-0.44435936052190783
2111	80-84	-0.19355049517174905
2111	85-89	-0.24669351121991667
2111	90-94	-0.5210433058163417
2111	95-99	-0.36133900801888785
2111	100-104	-0.4354146982529272
2111	105-109	-0.30763176639492684
2111	110-114	-0.40804010130701585
2111	115-119	-1.6400597911737975
2111	120-124	-0.7926437492218241
2111	125-129	-0.8056586109964101
2111	130-134	-0.5733463445304992
2111	135-139	-0.3653546266240326
2111	140-144	-0.8867323445145416
2111	145-149	-1.5615254710916773
2111	150-151	-2.5282787080009257
2110	1	0.9845308054589168
2110	2	0.36747129310667503
2110	3	0.08611463171038736
2110	4	0.09194233362865845
2110	5	0.13782500722897595
2110	6	1.1636026051653658
2110	7	0.15582814431817127
2110	8	-0.046828373522082245
2110	9	-0.044501919920620026
2110	10-14	0.03559306687546382
2110	15-19	-0.13937786505776728
2110	20-24	-0.15897283942426554
2110	25-29	-0.0889589973976399
2110	30-34	0.10916181527824875
2110	35-39	-0.24746571602023693
2110	40-44	-0.1981206964732749
2110	45-49	-0.05280478758309215
2110	50-54	-0.23086981083270075
2110	55-59	-0.38897358825556694
2110	60-64	-0.06573409709223199
2110	65-69	-0.12543673694468538
2110	70-74	-0.17429859421869054
2110	75-79	-0.31375617110949605
2110	80-84	-0.26046044182406547
2110	85-89	-0.20598582079720273
2110	90-94	-0.313779930731485
2110	95-99	-0.4506254073713194
2110	100-104	0.058952937817821294
2110	105-109	-0.16133311374542103
2110	110-114	-0.45050579488722065
2110	115-119	-6.998108191747448
2110	120-124	-2.94011395204749
2110	125-129	-0.43623787328787245
2110	130-134	-0.7067393157315891
2110	135-139	-0.626162906361948
2110	140-144	-0.6631584812661941
2110	145-149	-0.6922074216566969
2110	150-151	-1.0863283388252825
2109	1	0.9814752411034959
2109	2	0.48003199331440527
2109	3	0.061530827584313386
2109	4	0.04099891273416034
2109	5	0.08609563272836596
2109	6	1.1430625901703948
2109	7	0.41494166106079433
2109	8	0.11396903499326783
2109	9	-0.07725514104814124
2109	10-14	0.06811085391571936
2109	15-19	0.04584359233053448
2109	20-24	-0.24093784412339403
2109	25-29	-0.10055198908504082
2109	30-34	-0.034249726221666776
2109	35-39	-0.23042699108540887
2109	40-44	-0.04711193029974936
2109	45-49	-0.1868954875343718
2109	50-54	-0.01764919621999894
2109	55-59	-0.23823055284540828
2109	60-64	-0.25219238799765265
2109	65-69	-0.3023134588895857
2109	70-74	-0.3897490944784039
2109	75-79	-0.18795310805268173
2109	80-84	-0.22337446915996395
2109	85-89	-0.2639707674976748
2109	90-94	-0.38695804451322147
2109	95-99	-0.12134380818955748
2109	100-104	-0.22957933027934985
2109	105-109	-0.3130729475134615
2109	110-114	-0.7696235255077823
2109	115-119	-5.290171795156166
2109	120-124	-1.4881699111396784
2109	125-129	-0.5992048506651813
2109	130-134	-0.7654011731466284
2109	135-139	-1.0960371990414117
2109	140-144	-0.4711167769486835
2109	145-149	-0.5647911674522419
2109	150-151	-1.778178457522884
2108	1	0.8029514558708968
2108	2	0.18480525484590515
2108	3	0.06563687785844508
2108	4	0.04285151446948987
2108	5	0.037197873789217795
2108	6	0.9432449871524753
2108	7	-0.15267231294960482
2108	8	-0.07990613102801092
2108	9	-0.23185827641808032
2108	10-14	-0.17470516499769673
2108	15-19	-0.21192894989716393
2108	20-24	-0.5755282353697382
2108	25-29	-0.24269249932913084
2108	30-34	-7.4485860179913885
2108	35-39	-0.9878954214780009
2108	40-44	-0.4959654850688082
2108	45-49	-0.23329926063638595
2108	50-54	-0.31194519295246437
2108	55-59	-0.30851097615366285
2108	60-64	-0.5650589568483468
2108	65-69	-0.49029115170653625
2108	70-74	-0.2889422588133215
2108	75-79	-0.6353494444531336
2108	80-84	-0.31230282918816954
2108	85-89	-0.46507780002178123
2108	90-94	-0.7148456461346058
2108	95-99	-0.5452464024163177
2108	100-104	-0.6346936914049266
2108	105-109	-0.7589769184094166
2108	110-114	-0.927210953643133
2108	115-119	-0.6459255828955079
2108	120-124	-0.6784449937193884
2108	125-129	-0.7061434445577035
2108	130-134	-1.422695562401593
2108	135-139	-2.0241212506559876
2108	140-144	-1.5518022132961349
2108	145-149	-1.5643764721513307
2108	150-151	-1.717961450983708
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	4.0
19	17.0
20	96.0
21	261.0
22	660.0
23	1213.0
24	1819.0
25	2702.0
26	4059.0
27	5922.0
28	7900.0
29	10999.0
30	14832.0
31	20256.0
32	27686.0
33	38498.0
34	55407.0
35	86649.0
36	170041.0
37	614296.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.09873427476062958	0.13408596143102086	99.60872329358276	0.15845647022559578
2	22.117424669901816	34.39604258360606	26.260015799571157	17.226516946920963
3	0.0462701726667419	0.12000150472106233	0.12272881164654102	99.71099951096566
4	0.2457397584922695	35.0337621788361	0.15752548621299325	64.56297257645865
5	38.66634691344092	26.329327013504873	0.1147349810028966	34.88959109205131
6	60.770135048715346	22.61492307113569	0.3961178196591807	16.218824060489787
7	0.5232667494263251	42.528213519918744	0.30009780686905163	56.648421923785875
8	38.383741488921494	35.01025091223715	26.201143588007376	0.4048640108339916
9	22.16548169883008	16.588985441823723	35.173231012301095	26.072301847045104
10-14	25.79558364368205	44.60811420832863	24.077963360042133	5.518338787947184
15-19	29.63326185908287	29.211432118271073	12.230203513523682	28.925102509122368
20-24	28.592784862506115	20.761445284580372	20.967046608734904	29.678723244178613
25-29	31.138641236880716	37.99697174886206	4.574540119625325	26.289846894631907
30-34	24.799815671669865	16.739495166083586	28.860418312455327	29.600270849791222
35-39	38.69469209645262	12.27474325696874	19.157995711544974	29.872568935033666
40-44	10.34824888086371	30.392638152202533	32.753714780122635	26.505398186811117
45-49	5.545611857201972	27.20458563743746	27.18948199977429	40.060320505586276
50-54	26.06481585976	17.710585712673513	17.71735695745401	38.50724147011248
55-59	17.47660158748072	23.914381371553247	39.2464356919836	19.36258134898243
60-64	26.451378700673363	13.257909190083888	30.401741714629647	29.8889703946131
65-69	22.401948613775723	25.271526915698	25.452413196403718	26.87411127412256
70-74	20.203137343414966	14.62857841477636	39.843452582477525	25.324831659331153
75-79	34.630271225971484	28.128296279577174	24.000338562239023	13.241093932212317
80-84	14.24664259112967	17.470168904939246	41.11576947673325	27.16741902719783
85-89	25.022157017642854	32.341082646804345	33.530583455591916	9.106176879960877
90-94	24.576289357860286	30.421265470413424	15.540439378550202	29.46200579317609
95-99	4.7621412180717	22.770097430688786	41.00024451717263	31.467516834066885
100-104	22.48630703833277	31.835383515780762	13.323627882481285	32.35468156340519
105-109	19.14497987435579	39.509931159011394	23.561035248090885	17.784053718541927
110-114	34.79165255990671	30.633299477109432	17.02072753263364	17.554320430350224
115-119	21.54790655682203	37.28546439453787	11.042677651130422	30.123951397509686
120-124	0.4322687431817327	20.11783846819396	45.089925892487685	34.359966896136626
125-129	25.468062295451983	35.4448519730655	10.073618477974646	29.01346725350788
130-134	15.887691381710114	27.131569047887748	37.650961140578566	19.32977842982357
135-139	33.63916788925253	20.748015649099045	23.888594214347517	21.724222247300908
140-144	24.863653462739343	35.88629951472746	14.009084753413836	25.24096226911936
145-149	30.701350487153444	24.10115487341534	28.146860775683706	17.050633863747507
150-151	41.70245081443028	16.557856524846706	30.14261934318926	11.597073317533763
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.5
23	0.5
24	0.0
25	0.0
26	0.5
27	0.5
28	0.5
29	1.0
30	4.0
31	9.5
32	37.5
33	101.5
34	427.5
35	4186.5
36	104966.5
37	111735.5
38	11447.0
39	1059.5
40	334.5
41	259.5
42	316.0
43	386.5
44	504.0
45	1144.5
46	7792.0
47	77742.0
48	81021.5
49	19523.0
50	126070.0
51	128082.5
52	12517.0
53	1479.5
54	476.5
55	652.5
56	2083.0
57	17017.5
58	171680.0
59	166808.0
60	11699.0
61	1381.0
62	243.0
63	70.0
64	27.5
65	10.5
66	6.5
67	3.5
68	3.5
69	4.0
70	1.5
71	0.5
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.5
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.3680547718466687
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	1063320.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	0.9688247665170558
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.01335303640205	0.6879949517508548
2	9.62151089951646	0.18643116101530685
3	4.1873492536494625	0.12170422988976924
4	2.108293615172954	0.08170268277877347
5	1.4529574779715215	0.07038305946774846
6	0.9443158260779427	0.05489259358309941
7	0.7881623176419947	0.0534513821356933
8	0.6031957243877856	0.046751276547526616
9	0.5253408948758214	0.045806694283793595
>10	4.77662455536679	0.9907636147059556
>50	1.4592529580454205	1.0090479273714905
>100	2.0818675534781335	3.8095187909983443
>500	0.1751103549654505	1.1960413037870554
>1k	0.1751103549654505	4.500659128324623
>5k	0.04864176526818069	3.1052776072397252
>10k+	0.03891341221454455	84.03957359612023
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TACACGCATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC	314219	29.55074671782718	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	236211	22.2144791784223	No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	202270	19.02249557988188	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	138960	13.068502426362713	No Hit
NACACGCATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC	8127	0.7643042545988038	No Hit
TACACGCATGGTAACAGCATTCGTCGACGAAGCCTGGCCCATTGTCAACC	7075	0.6653688447504044	No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC	6255	0.5882518903058346	No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	6206	0.5836436820524395	No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	5284	0.49693413083549637	No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG	4674	0.43956664033404813	No Hit
TCCACGCATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC	4504	0.4235789790467592	No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	3947	0.3711958770642892	No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG	3845	0.3616032802919159	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG	3595	0.3380920136929617	No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	3569	0.33564684196667044	No Hit
TACACACATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC	3540	0.33291953504119176	No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	3396	0.31937704548019413	No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC	2968	0.2791257570627845	No Hit
TACACGCATGCTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC	1978	0.1860211413309258	No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	1933	0.181789113343114	No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	1806	0.16984538991084527	No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC	1627	0.15301132302599404	No Hit
TACACGCATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACA	1452	0.1365534364067261	No Hit
TACACGCATGATAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC	1406	0.13222736335251853	No Hit
TACACTCATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC	1287	0.12103600045141631	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA	1184	0.11134935861264716	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10-11	0.0	0.0	9.404506639581687E-4
12-13	0.0	0.0	9.404506639581687E-4
14-15	0.0	0.0	9.404506639581687E-4
16-17	0.0	0.0	9.404506639581687E-4
18-19	0.0	0.0	9.404506639581687E-4
20-21	0.0	0.0	9.404506639581687E-4
22-23	0.0	0.0	0.003573712523041041
24-25	0.0	0.0	0.0057367490501448294
26-27	0.0	0.0	0.005877816649738555
28-29	0.0	0.0	0.005924839182936463
30-31	0.0	0.0	0.006206974382123914
32-33	0.0	0.0	0.00663017718090509
34-35	0.0	0.0	0.0070533799796862655
36-37	0.0	0.0	0.007429560245269533
38-39	0.0	0.0	0.007617650378061167
40-41	0.0	0.0	0.009216416506790054
42-43	0.0	0.0	0.011849678365872926
44-45	0.0	0.0	0.014859120490539066
46-47	0.0	0.0	0.023464244065756312
48-49	0.0	0.0	0.03357408870330662
50-51	0.0	0.0	0.07072188992965428
52-53	0.0	0.0	0.0708159349960501
54-55	0.0	0.0	0.07086295752924801
56-57	0.0	0.0	0.07095700259564383
58-59	0.0	0.0	0.07138020539442501
60-61	0.0	0.0	0.07170936312681037
62-63	0.0	0.0	0.07316706165594553
64-65	0.0	0.0	0.07316706165594553
66-67	0.0	0.0	0.07363728698792461
68-69	0.0	0.0	0.07401346725350788
70-71	0.0	0.0	0.07401346725350788
72-73	0.0	0.0	0.07401346725350788
74-75	0.0	0.0	0.07415453485310161
76-77	0.0	0.0	0.07438964751909115
78-79	0.0	0.0	0.07438964751909115
80-81	0.0	0.0	0.07438964751909115
82-83	0.0	0.0	0.07438964751909115
84-85	0.0	0.0	0.07438964751909115
86-87	0.0	0.0	0.07438964751909115
88-89	0.0	0.0	0.07448369258548697
90-91	0.0	0.0	0.07448369258548697
92-93	0.0	0.0	0.07448369258548697
94-95	0.0	0.0	0.07448369258548697
96-97	0.0	0.0	0.07448369258548697
98-99	0.0	0.0	0.07448369258548697
100-101	0.0	0.0	0.07448369258548697
102-103	0.0	0.0	0.07584734604822632
104-105	0.0	0.0	0.07702290937817402
106-107	0.0	0.0	0.0779633600421322
108-109	0.0	0.0	0.0779633600421322
110-111	0.0	0.0	0.0780103825753301
112-113	0.0	0.0	0.07810442764172591
114-115	0.0	0.0	0.07819847270812173
116-117	0.0	0.0	0.07829251777451755
118-119	0.0	0.0	0.078574652973705
120-121	0.0	0.0	0.07927999097167363
122-123	0.0	0.0	0.12489184817364482
124-125	0.0	0.0	0.34768461046533494
126-127	0.0	0.0	0.3483429259301057
128-129	0.0	0.0	0.3483429259301057
130-131	0.0	0.0	0.3483429259301057
132-133	0.0	0.0	0.3484839935296994
134-135	0.0	0.0	0.34862506112929315
136-137	0.0	0.0	0.34862506112929315
138-139	0.0	0.0	0.34862506112929315
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCACGC	25	3.2194293E-8	148.49562	1
TGCCGCG	25	3.2194293E-8	148.49562	1
TGCCGAG	30	5.511538E-10	148.49562	1
ACCCGCA	15	1.11866044E-4	148.49562	1
TACACGC	34805	0.0	148.49562	1
TACATGC	15	1.11866044E-4	148.49562	1
TGCCCGG	10	0.006603439	148.49562	1
GTCCCGC	15	1.11866044E-4	148.49562	1
TCTCGCA	10	0.006603439	148.49562	1
TACCGCA	10	0.006603439	148.49562	1
TGCCAGA	10	0.006603439	148.49562	1
TCCCGAA	210	0.0	148.49562	1
GCCCGCA	10	0.006603439	148.49562	1
TGCAGAC	15	1.11866044E-4	148.49562	1
AGTTTGG	60	0.0	148.49562	1
TTCCGCA	70	0.0	148.4956	1
TCCCGCG	55	0.0	148.4956	1
TTCCAGA	26320	0.0	148.26994	1
TCCCGCA	15755	0.0	148.21284	1
ATCCCGA	25	3.715286E-8	144.9763	7
>>END_MODULE