FastQCFastQC Report
Wed 17 Jun 2015
000000000-AG1W8 l01n01 mis23.3410000000d2e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG1W8 l01n01 mis23.3410000000d2e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063320
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACACGCATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC31421929.55074671782718No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC23621122.2144791784223No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG20227019.02249557988188No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG13896013.068502426362713No Hit
NACACGCATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC81270.7643042545988038No Hit
TACACGCATGGTAACAGCATTCGTCGACGAAGCCTGGCCCATTGTCAACC70750.6653688447504044No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC62550.5882518903058346No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC62060.5836436820524395No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG52840.49693413083549637No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG46740.43956664033404813No Hit
TCCACGCATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC45040.4235789790467592No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG39470.3711958770642892No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG38450.3616032802919159No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG35950.3380920136929617No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG35690.33564684196667044No Hit
TACACACATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC35400.33291953504119176No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG33960.31937704548019413No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC29680.2791257570627845No Hit
TACACGCATGCTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC19780.1860211413309258No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC19330.181789113343114No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG18060.16984538991084527No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC16270.15301132302599404No Hit
TACACGCATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACA14520.1365534364067261No Hit
TACACGCATGATAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC14060.13222736335251853No Hit
TACACTCATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC12870.12103600045141631No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA11840.11134935861264716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACGC253.2194293E-8148.495621
TGCCGCG253.2194293E-8148.495621
TGCCGAG305.511538E-10148.495621
ACCCGCA151.11866044E-4148.495621
TACACGC348050.0148.495621
TACATGC151.11866044E-4148.495621
TGCCCGG100.006603439148.495621
GTCCCGC151.11866044E-4148.495621
TCTCGCA100.006603439148.495621
TACCGCA100.006603439148.495621
TGCCAGA100.006603439148.495621
TCCCGAA2100.0148.495621
GCCCGCA100.006603439148.495621
TGCAGAC151.11866044E-4148.495621
AGTTTGG600.0148.495621
TTCCGCA700.0148.49561
TCCCGCG550.0148.49561
TTCCAGA263200.0148.269941
TCCCGCA157550.0148.212841
ATCCCGA253.715286E-8144.97637
Produced by FastQC (version 0.11.2)