##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AG1W8 l01n01 mis22.3410000000d2dc.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 923372 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.311973938997504 34.0 34.0 34.0 31.0 34.0 2 33.47046152579892 34.0 34.0 34.0 34.0 34.0 3 33.55612797442418 34.0 34.0 34.0 34.0 34.0 4 33.57159844569686 34.0 34.0 34.0 34.0 34.0 5 33.71170990673315 34.0 34.0 34.0 34.0 34.0 6 36.40237845635345 38.0 38.0 38.0 34.0 38.0 7 36.837333165831325 38.0 38.0 38.0 34.0 38.0 8 36.94450449006467 38.0 37.0 38.0 35.0 38.0 9 37.418651421095724 38.0 38.0 38.0 37.0 38.0 10-14 37.3094183059482 38.0 38.0 38.0 36.6 38.0 15-19 37.26477822589379 38.0 38.0 38.0 36.2 38.0 20-24 37.516540245968045 38.0 38.0 38.0 37.4 38.0 25-29 37.26524802571445 38.0 38.0 38.0 36.8 38.0 30-34 36.928931784806124 38.0 37.8 38.0 35.6 38.0 35-39 37.11443600195804 38.0 38.0 38.0 36.0 38.0 40-44 36.94499616622553 38.0 38.0 38.0 35.6 38.0 45-49 37.10461027624836 38.0 38.0 38.0 36.2 38.0 50-54 37.050458320157 38.0 38.0 38.0 36.0 38.0 55-59 36.72940201782164 38.0 37.8 38.0 35.0 38.0 60-64 36.612767985167416 38.0 37.2 38.0 34.4 38.0 65-69 36.82949818707954 38.0 37.4 38.0 35.4 38.0 70-74 36.89380206460668 38.0 38.0 38.0 35.6 38.0 75-79 36.52155447641904 38.0 37.4 38.0 34.2 38.0 80-84 36.73475977179295 38.0 37.4 38.0 34.6 38.0 85-89 36.403509311523415 38.0 37.0 38.0 33.4 38.0 90-94 36.699211801960644 38.0 37.2 38.0 34.6 38.0 95-99 36.52394744480014 38.0 37.0 38.0 34.0 38.0 100-104 36.05677906629181 38.0 36.8 38.0 32.2 38.0 105-109 36.28961090438089 38.0 37.0 38.0 33.4 38.0 110-114 36.341011206750906 38.0 37.0 38.0 33.8 38.0 115-119 35.89732415537833 38.0 36.8 38.0 31.0 38.0 120-124 36.145488058983815 38.0 36.8 38.0 32.8 38.0 125-129 36.112036752251534 38.0 37.0 38.0 32.6 38.0 130-134 35.534749808311275 38.0 36.4 38.0 30.2 38.0 135-139 35.473549771922904 38.0 36.4 38.0 29.8 38.0 140-144 35.84791308378421 38.0 37.0 38.0 31.4 38.0 145-149 35.551335756336556 38.0 36.6 38.0 30.2 38.0 150-151 33.93318402550651 36.5 33.0 37.5 25.5 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6258004404947428 1101 2 0.24608773434764686 1101 3 0.16854270359240786 1101 4 0.036670625335823104 1101 5 0.13864626517416667 1101 6 1.0998047914835425 1101 7 -0.08821011081597874 1101 8 0.286228014902953 1101 9 0.11331122643073854 1101 10-14 0.20193231298949854 1101 15-19 0.22689298131468405 1101 20-24 0.12957361557325697 1101 25-29 0.22496477440354568 1101 30-34 0.18265244547408344 1101 35-39 0.01622428544521881 1101 40-44 0.06417995311054625 1101 45-49 0.20911430017145705 1101 50-54 0.16633269217821578 1101 55-59 0.3174607904838709 1101 60-64 0.4099633717585007 1101 65-69 0.2464285476518313 1101 70-74 0.16991253299959652 1101 75-79 0.1838200232177556 1101 80-84 0.29615043862080626 1101 85-89 0.34146210078751693 1101 90-94 0.3020837335708322 1101 95-99 0.3763172135561419 1101 100-104 0.5949291037408173 1101 105-109 0.39817521331423933 1101 110-114 0.3334212590591008 1101 115-119 0.7613731282062872 1101 120-124 0.5321177547089277 1101 125-129 0.3734345975911211 1101 130-134 0.4682439749201066 1101 135-139 0.6148784330944252 1101 140-144 0.5438456379673795 1101 145-149 0.6879344383329595 1101 150-151 0.6241652570346474 1102 1 0.5029619237740519 1102 2 0.24784639927205632 1102 3 0.085061142659832 1102 4 0.12774901993895327 1102 5 0.1709715871302464 1102 6 1.0619923011882122 1102 7 0.4516509701028326 1102 8 0.22940655928642428 1102 9 0.17682127827655592 1102 10-14 0.21289438694961405 1102 15-19 0.24206269654091983 1102 20-24 0.13129988781037838 1102 25-29 0.2516247523147328 1102 30-34 0.1593549686478326 1102 35-39 0.2168298726263771 1102 40-44 0.17619434349020935 1102 45-49 0.2756058488492741 1102 50-54 0.20498191393355825 1102 55-59 0.4380688302087137 1102 60-64 0.458632540338229 1102 65-69 0.33893091459535896 1102 70-74 0.19371835524273706 1102 75-79 0.21239241187585378 1102 80-84 0.39477913226704686 1102 85-89 0.23881614059676082 1102 90-94 0.39818340876805536 1102 95-99 0.40043613325842387 1102 100-104 0.7486956295454732 1102 105-109 0.3794064061661899 1102 110-114 0.3730433443741745 1102 115-119 0.7689146363136175 1102 120-124 0.45016670843627793 1102 125-129 0.48421105858652425 1102 130-134 0.5959754118320006 1102 135-139 0.7295778173307781 1102 140-144 0.45205935007538045 1102 145-149 0.6870226017042214 1102 150-151 1.0204780805733336 1103 1 1.0080174371036392 1103 2 0.36348697502898375 1103 3 0.2031926556200503 1103 4 0.19275679819818237 1103 5 0.20843130474510474 1103 6 1.2069179637609295 1103 7 0.5444019166352874 1103 8 0.3358690259298882 1103 9 0.16702895898197312 1103 10-14 0.16950661764071384 1103 15-19 0.3306532096692507 1103 20-24 0.14195011239726085 1103 25-29 0.24962309958262807 1103 30-34 0.40490341287759435 1103 35-39 0.20268627435866904 1103 40-44 0.36434307043660397 1103 45-49 0.21709979554037773 1103 50-54 0.2644868993596745 1103 55-59 0.42417044245124913 1103 60-64 0.4599273541217386 1103 65-69 0.31550208744319974 1103 70-74 0.3658257307373205 1103 75-79 0.33283329828959296 1103 80-84 0.36939989181347244 1103 85-89 0.303580981444334 1103 90-94 0.34599287575011317 1103 95-99 0.4303299369337381 1103 100-104 0.6248056001609399 1103 105-109 0.42803035278625146 1103 110-114 0.44050218589894996 1103 115-119 0.9048414804240466 1103 120-124 0.748184803248165 1103 125-129 0.5159937124764866 1103 130-134 0.6608395113179526 1103 135-139 0.6687150343477626 1103 140-144 0.77211541984056 1103 145-149 0.7650738932267558 1103 150-151 0.923349525009094 2116 1 0.5363452155834523 2116 2 0.26866302571241363 2116 3 -0.01915467146868366 2116 4 -0.4236626314157661 2116 5 -0.025391500692862223 2116 6 0.9473225137388184 2116 7 -0.1297656122183568 2116 8 0.07821777463990287 2116 9 0.04260945433801311 2116 10-14 0.22141182051132802 2116 15-19 0.26089872892958965 2116 20-24 0.11748108136379187 2116 25-29 0.2660205467193819 2116 30-34 0.25900126912535626 2116 35-39 0.13595770793209994 2116 40-44 0.028484615212406084 2116 45-49 0.30508452890740756 2116 50-54 0.28666142706549635 2116 55-59 0.44275172364793036 2116 60-64 0.4361576817791146 2116 65-69 0.4112523941448245 2116 70-74 0.2863184343977281 2116 75-79 0.3231874004567743 2116 80-84 0.41109012053653515 2116 85-89 0.43466861386573186 2116 90-94 0.40748888862894717 2116 95-99 0.5071335860249562 2116 100-104 0.7473670260470939 2116 105-109 0.45717342448862297 2116 110-114 0.3891332501667577 2116 115-119 0.8296346871804303 2116 120-124 0.6008561678466364 2116 125-129 0.4666895829299307 2116 130-134 0.6060816871356423 2116 135-139 0.7435866606298589 2116 140-144 0.752112714811183 2116 145-149 0.7996547321412635 2116 150-151 1.3296589237685978 1117 1 0.46733143211527306 1117 2 0.2844268248199313 1117 3 0.13331256412227788 1117 4 0.17983784297742744 1117 5 0.1557810845936487 1117 6 1.1508378455012647 1117 7 0.22621109038402665 1117 8 0.2162720587753597 1117 9 0.1790313827724077 1117 10-14 0.22816240959298995 1117 15-19 0.239138156738413 1117 20-24 0.15917411768874956 1117 25-29 0.2596606803835684 1117 30-34 0.3575091461376161 1117 35-39 0.21517472457234987 1117 40-44 0.20126500244237633 1117 45-49 0.3489630321186823 1117 50-54 0.3578271831752673 1117 55-59 0.5356096298536173 1117 60-64 0.5453224630369391 1117 65-69 0.3724135366504626 1117 70-74 0.35792925994614677 1117 75-79 0.3489701492264814 1117 80-84 0.4231704650189769 1117 85-89 0.39567166874937243 1117 90-94 0.34560520879743706 1117 95-99 -0.07358272163206436 1117 100-104 0.4694011562914753 1117 105-109 0.506066289823508 1117 110-114 0.49211554122523893 1117 115-119 0.9426940541001088 1117 120-124 0.6725608294114096 1117 125-129 0.6058397935396584 1117 130-134 0.649603352829196 1117 135-139 0.49688877611070836 1117 140-144 0.5578308470574171 1117 145-149 -0.42141471172394773 1117 150-151 0.18520815118539957 2117 1 -30.139641626521808 2117 2 -10.426580734011818 2117 3 -2.2327168763884764 2117 4 -0.8379833265979144 2117 5 -0.15667620614916444 2117 6 1.104946900106114 2117 7 -0.26203992615687355 2117 8 0.17960590906234586 2117 9 0.20060813988980897 2117 10-14 0.36856153012558224 2117 15-19 0.3673790926830094 2117 20-24 0.22502432067383893 2117 25-29 0.32064416856067623 2117 30-34 0.41715663220814037 2117 35-39 0.22442132923688973 2117 40-44 0.05172444500477269 2117 45-49 0.38353713500215747 2117 50-54 0.20757557213764954 2117 55-59 0.3535621379673657 2117 60-64 0.39805771329904616 2117 65-69 0.32460921653336783 2117 70-74 0.3322759193754621 2117 75-79 0.26320446816427534 2117 80-84 0.4567762490842 2117 85-89 0.282047597448539 2117 90-94 0.3521669661814002 2117 95-99 0.023829844542049727 2117 100-104 0.7841515403718873 2117 105-109 0.5360512981674717 2117 110-114 0.5392560605374896 2117 115-119 0.9764964421028992 2117 120-124 0.8036715851168736 2117 125-129 0.5328071656865063 2117 130-134 0.7296663080917156 2117 135-139 0.4995254013079986 2117 140-144 0.7380958181160864 2117 145-149 0.7979631345501943 2117 150-151 1.221789084319056 1116 1 -0.43496709615920537 1116 2 -0.25066694341912665 1116 3 -0.6642735969360203 1116 4 -0.9796754774643759 1116 5 -0.21128520571228648 1116 6 1.0416004606128837 1116 7 -0.6397157671354634 1116 8 0.1268900214840727 1116 9 0.06284783699189234 1116 10-14 0.23084491442731547 1116 15-19 0.2041637147887272 1116 20-24 0.03580049658763329 1116 25-29 0.013741591015893562 1116 30-34 0.24051472162127396 1116 35-39 0.12806192920776738 1116 40-44 0.019614353261658835 1116 45-49 0.18853203816219377 1116 50-54 0.058631346129210726 1116 55-59 0.25226608437774445 1116 60-64 0.3076543348222742 1116 65-69 0.21884250617950585 1116 70-74 0.19637960366263485 1116 75-79 0.15771444340818874 1116 80-84 0.31109400064966053 1116 85-89 0.38659106621219763 1116 90-94 0.33668713510522963 1116 95-99 -0.29594589449975217 1116 100-104 0.6572839125868271 1116 105-109 0.33628429656560144 1116 110-114 0.32539265584839683 1116 115-119 0.7601428670337285 1116 120-124 0.4859345820587677 1116 125-129 0.2946391155918633 1116 130-134 0.385323800446443 1116 135-139 0.5189516427525902 1116 140-144 0.5349357773413672 1116 145-149 0.5144040840186648 1116 150-151 0.5174422079538274 2118 1 0.5953125384841726 2118 2 0.34841358816128576 2118 3 0.1627977013051236 2118 4 0.08975043024646112 2118 5 0.1194892313035325 2118 6 1.2458902685058675 2118 7 0.6055236949600129 2118 8 0.2706059338457081 2118 9 0.1052982483637308 2118 10-14 0.2748150622620784 2118 15-19 0.2182603848629654 2118 20-24 0.12791637614468243 2118 25-29 0.2432675561695632 2118 30-34 0.32051550865253375 2118 35-39 0.048859386887855294 2118 40-44 0.1226357697403202 2118 45-49 0.214871466982963 2118 50-54 0.13091828658176752 2118 55-59 0.2665415080556457 2118 60-64 0.2465574465732132 2118 65-69 0.2534446139954767 2118 70-74 0.30544618633285836 2118 75-79 0.2937786010818897 2118 80-84 0.24828660791879997 2118 85-89 0.1674688881414923 2118 90-94 0.25859893850203264 2118 95-99 0.35374555268182917 2118 100-104 0.7237229670279675 2118 105-109 0.3699614337080632 2118 110-114 0.49116334314782506 2118 115-119 0.8965895706525657 2118 120-124 0.5966064995472564 2118 125-129 0.5348492738321511 2118 130-134 0.5575059020671489 2118 135-139 0.6601555324282273 2118 140-144 0.481377394857752 2118 145-149 0.4075454374442842 2118 150-151 0.9768259973038695 1119 1 1.1153121383394833 1119 2 0.3481968431241711 1119 3 0.12067457573287754 1119 4 -0.11737095171287848 1119 5 0.10572285016115757 1119 6 1.2530568948948115 1119 7 0.2137618666919323 1119 8 0.014683748648565143 1119 9 0.062281115326676684 1119 10-14 0.16193698476774898 1119 15-19 0.15727321037759623 1119 20-24 -0.02955024468084133 1119 25-29 0.13237524931967215 1119 30-34 0.1806616421931011 1119 35-39 0.12494865355077422 1119 40-44 0.16231509940671174 1119 45-49 0.20289987514949814 1119 50-54 0.06933903836587518 1119 55-59 0.25182113284629537 1119 60-64 0.24785700418519951 1119 65-69 0.1778755705220263 1119 70-74 0.195849470748513 1119 75-79 0.19545406654405184 1119 80-84 0.23319100931727377 1119 85-89 0.2777771592549598 1119 90-94 0.23967304266496825 1119 95-99 0.22766371853091982 1119 100-104 0.5493376322604391 1119 105-109 0.2605817088636897 1119 110-114 0.21933017680469646 1119 115-119 0.7239397405999739 1119 120-124 0.5335424337508314 1119 125-129 0.25610989104830395 1119 130-134 0.5616144051592329 1119 135-139 0.402069512202317 1119 140-144 0.5310616403366879 1119 145-149 0.470862893106748 1119 150-151 0.8384828174277814 2119 1 0.755321278593378 2119 2 0.3105863145695622 2119 3 0.13463512718110593 2119 4 0.1306144871542827 2119 5 0.11520010363521749 2119 6 1.2324725823283913 2119 7 0.4770056492646191 2119 8 0.2819761685135944 2119 9 0.11022669883543301 2119 10-14 0.2521138424908216 2119 15-19 0.19314430528750393 2119 20-24 0.12157291817712235 2119 25-29 0.17625115025623472 2119 30-34 0.18803447084548708 2119 35-39 0.19146397989617725 2119 40-44 -0.006555183437370715 2119 45-49 0.17220193010759743 2119 50-54 0.13681543249101225 2119 55-59 0.28881543359882045 2119 60-64 0.15433594145358853 2119 65-69 0.23619180026702935 2119 70-74 0.167976407485952 2119 75-79 -0.007911355682892918 2119 80-84 0.17531397943211147 2119 85-89 0.32292791584481506 2119 90-94 0.281126569993134 2119 95-99 0.3772212432415074 2119 100-104 0.7458281599072123 2119 105-109 0.3733530816435078 2119 110-114 0.4510741616630938 2119 115-119 0.8806145932615266 2119 120-124 0.53044104037793 2119 125-129 0.5642378910538 2119 130-134 0.5391694766504358 2119 135-139 0.33894154744421456 2119 140-144 0.27378563888483853 2119 145-149 0.4143198313521026 2119 150-151 0.3853836162715254 1118 1 0.3821822238289343 1118 2 0.3021509831107849 1118 3 0.13177749563555352 1118 4 0.1904699910357266 1118 5 0.18179726843377608 1118 6 1.2358876186532868 1118 7 0.4698015386820842 1118 8 0.5430427761782326 1118 9 0.1606331036796078 1118 10-14 0.2043337987079923 1118 15-19 0.1881913613289825 1118 20-24 0.10021538265188923 1118 25-29 0.22901569839924463 1118 30-34 0.19914893649623622 1118 35-39 0.10755138486117488 1118 40-44 0.11485272090629906 1118 45-49 0.20907997030708003 1118 50-54 0.22460831347482468 1118 55-59 0.3339172562196282 1118 60-64 0.3784806834815413 1118 65-69 0.12599906877665745 1118 70-74 0.17547567021427568 1118 75-79 -0.01577978685093484 1118 80-84 -0.2713602937884403 1118 85-89 0.24660314744245682 1118 90-94 0.23010889160306647 1118 95-99 0.14927519235469333 1118 100-104 0.3533833913709614 1118 105-109 0.36097035653521914 1118 110-114 0.3127271475905715 1118 115-119 0.7825121433891482 1118 120-124 0.49818606641905916 1118 125-129 0.30520167937329745 1118 130-134 0.4443044483449867 1118 135-139 0.6535600089705014 1118 140-144 0.4267711402737149 1118 145-149 0.6546683592282676 1118 150-151 0.8511965796532408 2112 1 0.7462279386955828 2112 2 0.2519934645813251 2112 3 0.1334712888124514 2112 4 0.10412678097389971 2112 5 0.1523371279439516 2112 6 1.2104574230228948 2112 7 0.49740268215889216 2112 8 0.5015766133126718 2112 9 0.052784348094142786 2112 10-14 0.12268419301868505 2112 15-19 0.1382034036598938 2112 20-24 0.055264568614525444 2112 25-29 0.14992408954383762 2112 30-34 0.2915124043002493 2112 35-39 -0.0494363138070284 2112 40-44 0.0890962325710305 2112 45-49 0.022391042473927314 2112 50-54 0.05142319561731057 2112 55-59 0.22328508830958782 2112 60-64 0.26600761231196657 2112 65-69 0.09525094350736651 2112 70-74 0.05832164214535851 2112 75-79 0.18518989310143752 2112 80-84 0.00330367071633475 2112 85-89 0.23467167859276117 2112 90-94 0.1473788649283989 2112 95-99 0.12196941881344969 2112 100-104 0.28377853716182955 2112 105-109 0.09551060575700632 2112 110-114 0.25526852721809234 2112 115-119 0.6245615423618673 2112 120-124 0.33917598232849855 2112 125-129 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-0.5231348837330358 2108 25-29 -0.3288443603326172 2108 30-34 -6.054164814693941 2108 35-39 -0.9362292076826719 2108 40-44 -0.6914913250326649 2108 45-49 -0.31414305222986627 2108 50-54 -0.2933198418501348 2108 55-59 -0.49781372288389747 2108 60-64 -0.5303374755127876 2108 65-69 -0.43191295150365505 2108 70-74 -0.3278058874078411 2108 75-79 -0.5368364209304914 2108 80-84 -0.32932088682056815 2108 85-89 -0.7301783848547743 2108 90-94 -0.782679406627075 2108 95-99 -0.6119290985177486 2108 100-104 -0.46832775416254435 2108 105-109 -0.6148789287384204 2108 110-114 -0.9211239792936325 2108 115-119 -0.8446931160232296 2108 120-124 -0.9016708753486569 2108 125-129 -0.8345017171119054 2108 130-134 -1.1125569721000943 2108 135-139 -1.655301584922661 2108 140-144 -1.5398895504504964 2108 145-149 -1.2813963077568715 2108 150-151 -1.7754391068531916 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 6.0 19 28.0 20 72.0 21 221.0 22 597.0 23 1048.0 24 1543.0 25 2065.0 26 2853.0 27 4043.0 28 5504.0 29 7597.0 30 10457.0 31 14349.0 32 20174.0 33 28625.0 34 42361.0 35 71401.0 36 149699.0 37 560729.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.13089801794066325 18.156650515570004 80.6224460778727 1.0900053886166303 2 44.141039581013935 0.055232344060681934 31.595283374414645 24.208444700510736 3 0.16353105790515632 0.049817408368458216 18.82339945330809 80.96325208041829 4 0.5932603544400307 0.26619823862971803 18.66669121437514 80.47385019255512 5 49.50020143560775 31.57481491749804 18.696581659396212 0.22840198749799648 6 50.46525127467586 25.860000086638973 0.4118600087505361 23.26288862993463 7 18.609509493465257 55.25021334846627 0.9210805612472546 25.219196596821213 8 49.11628249502909 19.203961133757574 31.233240774032566 0.4465155971807679 9 25.233816923190222 42.827701078222 0.6268329557318177 31.311649042855965 10-14 16.100769787258006 40.228033771870926 25.942263789675234 17.728932651195834 15-19 31.267593126064035 24.580948956650193 22.530074552834613 21.621383364451162 20-24 27.41722729300867 26.138479399418653 15.173797775977613 31.270495531595067 25-29 29.897289499789903 38.29325558929662 12.768093466121996 19.041361444791484 30-34 18.330791923515115 15.14228285024887 38.789458636389234 27.737466589846775 35-39 35.85387037943538 15.177176695849559 18.809818794591994 30.15913413012307 40-44 12.177930454897917 32.49061050150968 25.72129109394697 29.610167949645426 45-49 10.355891233435711 19.130837842169786 38.11194188257821 32.401329041816304 50-54 18.726168868018522 32.737964763930464 16.621599961878854 31.91426640617216 55-59 20.051441889076123 30.78594542611212 42.56161113830612 6.601001546505633 60-64 25.46561949030293 27.64255359703348 20.08798187512725 26.803845037536334 65-69 18.596167091919614 38.74501284422746 22.642726874975633 20.01609318887729 70-74 16.329756587810763 13.715815510974991 38.7875309192828 31.16689698193144 75-79 26.02151678846662 27.687237646365716 25.86606481461426 20.425180750553405 80-84 4.157371027061683 28.990114493400277 32.7366868391071 34.11582764043094 85-89 26.027018363129915 33.85406964906885 29.68489406219812 10.434017925603117 90-94 12.73566883119696 48.73483276512608 23.501643974476156 15.027854429200799 95-99 9.157067790662918 42.562044333161495 35.48632620439 12.794561671785587 100-104 18.764376654262854 38.30568828164596 16.581637736470242 26.34829732762094 105-109 15.06034404335414 35.72007814835191 32.6103022400506 16.60927556824335 110-114 37.24126354275417 11.809909765511625 25.847459095575783 25.10136759615843 115-119 13.79621647613313 44.71818508683391 16.591709516857776 24.893888920175183 120-124 4.148880407896276 20.289958976447195 47.721395060712254 27.839765554944268 125-129 22.306762604887304 34.812469947106905 9.044502107492972 33.83626534051282 130-134 18.708797754317867 54.0139835299316 20.642904484866335 6.634314230884194 135-139 32.22419566545228 11.844760291626777 37.96816451007828 17.962879532842667 140-144 14.7310726337814 37.80309561043653 25.05921773673016 22.40661401905191 145-149 24.120137929241952 33.41513496185719 30.70948653413792 11.755240574762935 150-151 36.922713705852026 33.68550270096992 16.167752541770813 13.224031051407234 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 1.0 29 3.5 30 4.0 31 8.5 32 36.5 33 115.0 34 492.0 35 4347.5 36 104662.5 37 111562.0 38 11783.0 39 1680.0 40 4804.0 41 49815.0 42 74394.0 43 35238.5 44 8533.5 45 3399.5 46 10422.5 47 98335.5 48 101866.5 49 23270.5 50 130426.5 51 132204.5 52 13703.0 53 1365.5 54 297.0 55 155.0 56 98.0 57 41.5 58 80.0 59 89.0 60 54.5 61 32.5 62 13.0 63 13.5 64 8.5 65 8.5 66 5.5 67 0.5 68 0.5 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.124279272059365 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 923372.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 1.1639271513987917 #Duplication Level Percentage of deduplicated Percentage of total 1 71.30684437740778 0.8299597225143318 2 10.094735931808433 0.23499074474465628 3 3.7936835612867896 0.13246713902390872 4 1.9757312150136774 0.09198428820082173 5 1.312099763620469 0.07635942701109 6 0.9064770314468047 0.06330439374121874 7 0.8682532719443045 0.07074084902547702 8 0.5879793627550595 0.054749211581821854 9 0.44793495921253623 0.04692272949793631 >10 4.871031956798732 1.2227480512850444 >50 1.5396903013051044 1.3009468021570432 >100 1.894285643423856 4.738061721263888 >500 0.17729767105937566 1.4938405674459818 >1k 0.1586347583162835 5.390598738074667 >5k 0.027994369114638262 1.9338251638342088 >10k+ 0.03732582548618435 82.31850045059791 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC 241947 26.202548918529047 No Hit TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG 201132 21.78233691296682 No Hit TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG 175713 19.029491905754128 No Hit AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA 139126 15.067166862326342 No Hit TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC 6440 0.697443717158415 No Hit NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC 6290 0.6811989100817438 No Hit NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG 5075 0.5496159727607075 No Hit TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG 4992 0.5406271795116161 No Hit NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG 4802 0.520050423881166 No Hit TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG 4680 0.5068379807921402 No Hit TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG 4640 0.5025060322383612 No Hit TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG 4464 0.48344545860173366 No Hit TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG 3496 0.3786123036002825 No Hit CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA 3446 0.37319736790805874 No Hit TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC 3445 0.37308906919421425 No Hit AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCCTCCGCGGCTATATTGA 2776 0.3006372296322609 No Hit TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG 2302 0.24930363926998003 No Hit TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC 2087 0.22601941579341805 No Hit AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA 1708 0.18497420324636224 No Hit TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC 1637 0.17728499456340457 No Hit AGTTTAGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA 1575 0.17057047430504715 No Hit AGTTTGTAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA 1264 0.13688957429941562 No Hit TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA 1197 0.12963356047183583 No Hit TTCCATATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC 1121 0.1214028582196558 No Hit TCCCGCAGATCATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG 987 0.10689083056449622 No Hit TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG 985 0.10667423313680727 No Hit TCCCGTAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG 962 0.10418336271838435 No Hit CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA 938 0.10158419358611698 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10-11 0.0 0.0 0.0 12-13 0.0 0.0 0.0 14-15 0.0 0.0 0.0 16-17 0.0 0.0 0.0 18-19 0.0 0.0 0.0 20-21 0.0 0.0 8.122403538335578E-4 22-23 0.0 0.0 0.00173277942151159 24-25 0.0 0.0 0.0021118249199672504 26-27 0.0 0.0 0.0021659742768894877 28-29 0.0 0.0 0.0021659742768894877 30-31 0.0 0.0 0.002274272990733962 32-33 0.0 0.0 0.003032363987645283 34-35 0.0 0.0 0.004115351126090026 36-37 0.0 0.0 0.006227176046057277 38-39 0.0 0.0 0.00812240353833558 40-41 0.0 0.0 0.008447299679869002 42-43 0.0 0.0 0.010667423313680726 44-45 0.0 0.0 0.01640725514743787 46-47 0.0 0.0 0.04938421351308032 48-49 0.0 0.0 0.14262940613317276 50-51 0.0 0.0 0.18194183925871696 52-53 0.0 0.0 0.18237503411409486 54-55 0.0 0.0 0.18237503411409486 56-57 0.0 0.0 0.18356631996638406 58-59 0.0 0.0 0.1843244109632954 60-61 0.0 0.0 0.18443270967713987 62-63 0.0 0.0 0.1853532487448179 64-65 0.0 0.0 0.18584059295711805 66-67 0.0 0.0 0.1858947423140403 68-69 0.0 0.0 0.1889812556586078 70-71 0.0 0.0 0.1889812556586078 72-73 0.0 0.0 0.1889812556586078 74-75 0.0 0.0 0.1889812556586078 76-77 0.0 0.0 0.1889812556586078 78-79 0.0 0.0 0.1889812556586078 80-81 0.0 0.0 0.1889812556586078 82-83 0.0 0.0 0.1889812556586078 84-85 0.0 0.0 0.1889812556586078 86-87 0.0 0.0 0.1889812556586078 88-89 0.0 0.0 0.1893061518001412 90-91 0.0 0.0 0.1893061518001412 92-93 0.0 0.0 0.1893061518001412 94-95 0.0 0.0 0.1893061518001412 96-97 0.0 0.0 0.1894144505139857 98-99 0.0 0.0 0.1894144505139857 100-101 0.0 0.0 0.18952274922783016 102-103 0.0 0.0 0.1895768985847524 104-105 0.0 0.0 0.18963104794167465 106-107 0.0 0.0 0.18963104794167465 108-109 0.0 0.0 0.1896851972985969 110-111 0.0 0.0 0.1898476453693636 112-113 0.0 0.0 0.1898476453693636 114-115 0.0 0.0 0.1898476453693636 116-117 0.0 0.0 0.1900100934401303 118-119 0.0 0.0 0.19298830807085335 120-121 0.0 0.0 0.1967246136984877 122-123 0.0 0.0 0.22201236338117247 124-125 0.0 0.0 0.5318549836902137 126-127 0.0 0.0 0.533533613754803 128-129 0.0 0.0 0.5344000034655588 130-131 0.0 0.0 0.5345083021794033 132-133 0.0 0.0 0.5346707502501701 134-135 0.0 0.0 0.5348331983209367 136-137 0.0 0.0 0.5349956463917034 138-139 0.0 0.0 0.5351039451055479 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTTGT 60 0.0 148.12299 1 CGTTTGG 490 0.0 148.12299 1 TCCCACA 60 0.0 148.12299 1 AGCTTGG 20 1.9161016E-6 148.12299 1 TTCCGGA 25 3.2678145E-8 148.12299 1 TTCCGCA 45 0.0 148.12299 1 TGCCGCG 35 9.094947E-12 148.12299 1 AGGGTGT 15 1.1298658E-4 148.12299 1 ACCCGCA 15 1.1298658E-4 148.12299 1 TACACGC 15 1.1298658E-4 148.12299 1 CGGGTGT 35 9.094947E-12 148.12299 1 CCCCGCA 30 5.6024874E-10 148.12299 1 TCTCGCA 10 0.0066530905 148.12299 1 AGTGTGG 20 1.9161016E-6 148.12299 1 AGTTGGG 55 0.0 148.12299 1 CGTGTGT 100 0.0 148.12299 1 CGTGTGG 85 0.0 148.12299 1 TGGGTGT 10 0.0066530905 148.12299 1 TCCGCAT 10 0.0066530905 148.12299 1 TCCCGTA 85 0.0 148.12299 1 >>END_MODULE