FastQCFastQC Report
Wed 17 Jun 2015
000000000-AG1W8 l01n01 mis22.3410000000d2dc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG1W8 l01n01 mis22.3410000000d2dc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923372
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC24194726.202548918529047No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG20113221.78233691296682No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG17571319.029491905754128No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA13912615.067166862326342No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC64400.697443717158415No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC62900.6811989100817438No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG50750.5496159727607075No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG49920.5406271795116161No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG48020.520050423881166No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG46800.5068379807921402No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG46400.5025060322383612No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG44640.48344545860173366No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG34960.3786123036002825No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA34460.37319736790805874No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC34450.37308906919421425No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCCTCCGCGGCTATATTGA27760.3006372296322609No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG23020.24930363926998003No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC20870.22601941579341805No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA17080.18497420324636224No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC16370.17728499456340457No Hit
AGTTTAGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA15750.17057047430504715No Hit
AGTTTGTAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA12640.13688957429941562No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA11970.12963356047183583No Hit
TTCCATATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC11210.1214028582196558No Hit
TCCCGCAGATCATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG9870.10689083056449622No Hit
TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG9850.10667423313680727No Hit
TCCCGTAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG9620.10418336271838435No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA9380.10158419358611698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTGT600.0148.122991
CGTTTGG4900.0148.122991
TCCCACA600.0148.122991
AGCTTGG201.9161016E-6148.122991
TTCCGGA253.2678145E-8148.122991
TTCCGCA450.0148.122991
TGCCGCG359.094947E-12148.122991
AGGGTGT151.1298658E-4148.122991
ACCCGCA151.1298658E-4148.122991
TACACGC151.1298658E-4148.122991
CGGGTGT359.094947E-12148.122991
CCCCGCA305.6024874E-10148.122991
TCTCGCA100.0066530905148.122991
AGTGTGG201.9161016E-6148.122991
AGTTGGG550.0148.122991
CGTGTGT1000.0148.122991
CGTGTGG850.0148.122991
TGGGTGT100.0066530905148.122991
TCCGCAT100.0066530905148.122991
TCCCGTA850.0148.122991
Produced by FastQC (version 0.11.2)