##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AG1W8 l01n01 mis21.3410000000d2cf.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	917102
Sequences flagged as poor quality	0
Sequence length	151
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.189235221382134	34.0	34.0	34.0	31.0	34.0
2	33.45151357209994	34.0	34.0	34.0	33.0	34.0
3	33.508292425488115	34.0	34.0	34.0	33.0	34.0
4	33.53443237502481	34.0	34.0	34.0	34.0	34.0
5	33.708549321667604	34.0	34.0	34.0	34.0	34.0
6	36.434842580214635	38.0	38.0	38.0	34.0	38.0
7	36.78924154565141	38.0	38.0	38.0	34.0	38.0
8	37.00799583906697	38.0	38.0	38.0	35.0	38.0
9	37.438767988729715	38.0	38.0	38.0	37.0	38.0
10-14	37.31851353502664	38.0	38.0	38.0	36.8	38.0
15-19	37.326673368938245	38.0	38.0	38.0	36.6	38.0
20-24	37.555949283721986	38.0	38.0	38.0	37.4	38.0
25-29	37.31421368615487	38.0	38.0	38.0	36.8	38.0
30-34	36.96072825051085	38.0	37.8	38.0	35.8	38.0
35-39	37.19911111304958	38.0	38.0	38.0	36.0	38.0
40-44	36.962037810407125	38.0	38.0	38.0	35.6	38.0
45-49	37.15709921033865	38.0	38.0	38.0	36.4	38.0
50-54	37.10236614902159	38.0	38.0	38.0	36.0	38.0
55-59	36.87837470641215	38.0	37.8	38.0	35.4	38.0
60-64	36.73171708272362	38.0	37.2	38.0	35.0	38.0
65-69	36.9295373906065	38.0	37.8	38.0	35.6	38.0
70-74	36.98427721234934	38.0	38.0	38.0	36.0	38.0
75-79	36.622774129813266	38.0	37.4	38.0	34.4	38.0
80-84	36.84828361512678	38.0	37.8	38.0	35.4	38.0
85-89	36.53613861925936	38.0	37.0	38.0	33.8	38.0
90-94	36.84449058010996	38.0	37.6	38.0	35.2	38.0
95-99	36.68053738842572	38.0	37.2	38.0	34.6	38.0
100-104	36.215552686615005	38.0	37.2	38.0	32.6	38.0
105-109	36.47789733312107	38.0	37.0	38.0	34.0	38.0
110-114	36.56154277277773	38.0	37.2	38.0	34.0	38.0
115-119	36.101219057422185	38.0	37.2	38.0	32.0	38.0
120-124	36.3521529775314	38.0	36.8	38.0	33.6	38.0
125-129	36.39359525985114	38.0	37.0	38.0	34.0	38.0
130-134	35.90898962165605	38.0	36.8	38.0	31.6	38.0
135-139	35.76732947916371	38.0	37.0	38.0	31.6	38.0
140-144	36.03019860386304	38.0	37.0	38.0	32.0	38.0
145-149	35.77673301333985	38.0	36.8	38.0	31.2	38.0
150-151	34.3497190061738	37.0	34.0	37.5	26.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.6158956292439584
1101	2	0.340396799911197
1101	3	0.1338832974467934
1101	4	0.1369375791585341
1101	5	0.18146749513113747
1101	6	1.1435201336939116
1101	7	-0.07975052160165319
1101	8	0.2581555242922704
1101	9	0.18271610965075524
1101	10-14	0.22708490042919038
1101	15-19	0.2576299033181684
1101	20-24	0.12171783284220794
1101	25-29	0.20096224466104928
1101	30-34	0.24695139877439942
1101	35-39	0.042711579226498486
1101	40-44	0.07962693966885581
1101	45-49	0.20404119003735843
1101	50-54	0.20337022790640447
1101	55-59	0.32687505158293106
1101	60-64	0.3791631350343536
1101	65-69	0.17585431397817075
1101	70-74	0.1333842205585043
1101	75-79	0.17594723471999174
1101	80-84	0.2801021573125908
1101	85-89	0.3153742180273156
1101	90-94	0.31535899593930594
1101	95-99	0.4585064360590678
1101	100-104	0.5953782636870457
1101	105-109	0.38840494401293313
1101	110-114	0.36668613897160895
1101	115-119	0.6996365923250067
1101	120-124	0.4800063015929865
1101	125-129	0.2670580857532059
1101	130-134	0.4013167567274323
1101	135-139	0.40074748736449806
1101	140-144	0.39122365762790423
1101	145-149	0.48440027827848553
1101	150-151	0.4384136616725627
1102	1	0.31233594484824323
1102	2	0.28849383351852964
1102	3	0.11238917066548737
1102	4	0.1200153410093634
1102	5	0.20473971603038876
1102	6	1.0949099891573866
1102	7	0.28767608707713066
1102	8	0.12609220949737932
1102	9	0.14070315275048983
1102	10-14	0.21865443871840284
1102	15-19	0.21993229607675602
1102	20-24	0.10640043100348606
1102	25-29	0.25483513220542164
1102	30-34	0.11397538836770593
1102	35-39	0.13276391607952576
1102	40-44	0.14734712682555795
1102	45-49	0.22313282834444692
1102	50-54	0.15180909359384032
1102	55-59	0.27236721523752294
1102	60-64	0.383417829618395
1102	65-69	0.2725270912285893
1102	70-74	0.15396202363763223
1102	75-79	0.0798162632954913
1102	80-84	0.336043650067289
1102	85-89	0.15332711090168516
1102	90-94	0.3320218256255316
1102	95-99	0.35210724321023434
1102	100-104	0.7128153278626854
1102	105-109	0.3720557462496856
1102	110-114	0.24989184048266821
1102	115-119	0.6754339641363174
1102	120-124	0.4589532516552808
1102	125-129	0.4169107193180608
1102	130-134	0.48382454557785337
1102	135-139	0.45274593849972433
1102	140-144	0.29742383322747656
1102	145-149	0.5251665903377969
1102	150-151	1.0180846301550162
1103	1	0.9003401842913945
1103	2	0.3161019976769879
1103	3	0.22363413291778755
1103	4	0.23219289634281637
1103	5	0.18377171200847187
1103	6	1.1340134790684147
1103	7	0.5920048286543746
1103	8	0.24274556229531186
1103	9	0.15383071930914127
1103	10-14	0.15502944860031675
1103	15-19	0.2566125049581274
1103	20-24	0.12085534779816243
1103	25-29	0.23331640315528546
1103	30-34	0.2785482469383922
1103	35-39	0.1333173358066304
1103	40-44	0.3295057202446685
1103	45-49	0.20360402079578677
1103	50-54	0.2008446267700421
1103	55-59	0.30912128484159496
1103	60-64	0.3879825521463971
1103	65-69	0.26445516939043756
1103	70-74	0.20866986551984468
1103	75-79	0.22739446759684512
1103	80-84	0.30782843193385645
1103	85-89	0.23351079846076317
1103	90-94	0.2684181983010845
1103	95-99	0.3013562582247431
1103	100-104	0.4587540838500743
1103	105-109	0.26472481790805347
1103	110-114	0.26892735580243965
1103	115-119	0.6951811072279099
1103	120-124	0.5016594241228276
1103	125-129	0.28670666730257466
1103	130-134	0.4579172558816538
1103	135-139	0.47012285041699897
1103	140-144	0.5599699698003349
1103	145-149	0.6243323862325383
1103	150-151	0.6967059118154921
2116	1	0.41383676515071954
2116	2	0.2244430759430358
2116	3	-0.11081908740295177
2116	4	-0.4456666979404105
2116	5	0.0011222010962583795
2116	6	0.8582819900289707
2116	7	-0.20221525176494026
2116	8	0.05375520555074331
2116	9	0.0339377896470765
2116	10-14	0.13046700158783153
2116	15-19	0.2012653817021146
2116	20-24	0.07865721762090061
2116	25-29	0.18935959331107455
2116	30-34	0.2092315848369779
2116	35-39	0.07356967377069878
2116	40-44	-0.016866137315304286
2116	45-49	0.15952290349596154
2116	50-54	0.16874323768736588
2116	55-59	0.3038781568052613
2116	60-64	0.24232842787116482
2116	65-69	0.21267885150343346
2116	70-74	0.17392792004989133
2116	75-79	0.11014487118469418
2116	80-84	0.27511325336273273
2116	85-89	0.27933002607470314
2116	90-94	0.2706639945253073
2116	95-99	0.28620706699455667
2116	100-104	0.544282241408574
2116	105-109	0.24470343263756433
2116	110-114	0.2305378127129103
2116	115-119	0.6655965155929948
2116	120-124	0.36721018054364407
2116	125-129	0.3573917056009961
2116	130-134	0.3939320671869737
2116	135-139	0.6690460790786119
2116	140-144	0.5661894221687476
2116	145-149	0.565424639437154
2116	150-151	1.1110399567341673
1117	1	0.5033832492687225
1117	2	0.2583081678605481
1117	3	0.12022872875787982
1117	4	0.13309434690030741
1117	5	0.13970263487485113
1117	6	1.1347107345397731
1117	7	0.1752868589999963
1117	8	0.13371781584381637
1117	9	0.13966346821571562
1117	10-14	0.2322651040740098
1117	15-19	0.1953021514689155
1117	20-24	0.10160853299161943
1117	25-29	0.23536591559570041
1117	30-34	0.273526969044525
1117	35-39	0.15470271443890482
1117	40-44	0.09645691430886671
1117	45-49	0.2751213700761568
1117	50-54	0.19584366453478452
1117	55-59	0.35549742244545257
1117	60-64	0.4378589219523903
1117	65-69	0.25311841528098
1117	70-74	0.20346074891672572
1117	75-79	0.17605306619935135
1117	80-84	0.28043231196502205
1117	85-89	0.26573925420784406
1117	90-94	0.193958486101927
1117	95-99	-0.24491109649152776
1117	100-104	0.38665287033023077
1117	105-109	0.3548195713080915
1117	110-114	0.3571678996909142
1117	115-119	0.8106892891484705
1117	120-124	0.49064239823789535
1117	125-129	0.4160183656494496
1117	130-134	0.43031392268570556
1117	135-139	0.3000397022558161
1117	140-144	0.37101591142300094
1117	145-149	-0.5992019087735443
1117	150-151	0.028042539357748808
2117	1	-30.036632918880024
2117	2	-10.42185075626822
2117	3	-2.1095983723181178
2117	4	-0.7276920434455931
2117	5	-0.09596547104950304
2117	6	1.162987656909678
2117	7	-0.18032843932741827
2117	8	0.16651931317314705
2117	9	0.18867865248874693
2117	10-14	0.3814663682811954
2117	15-19	0.3546186189581917
2117	20-24	0.21444888401173756
2117	25-29	0.32964698653711366
2117	30-34	0.4369374039760672
2117	35-39	0.24893762051114976
2117	40-44	0.19700270421902388
2117	45-49	0.3684698271041782
2117	50-54	0.23235568933429107
2117	55-59	0.46828279340419954
2117	60-64	0.48122289956764774
2117	65-69	0.4178400578312065
2117	70-74	0.38000991450238786
2117	75-79	0.35302582057091314
2117	80-84	0.44361902483833404
2117	85-89	0.43988437900964783
2117	90-94	0.325903823554583
2117	95-99	0.04067280554703956
2117	100-104	0.7599186483753826
2117	105-109	0.5557020932050776
2117	110-114	0.5609398410594437
2117	115-119	0.9431028762423637
2117	120-124	0.7844135249322619
2117	125-129	0.4507781307726262
2117	130-134	0.7546675414056594
2117	135-139	0.5660446028342392
2117	140-144	0.753165857424726
2117	145-149	0.8892827346185044
2117	150-151	1.2239778375180492
1116	1	-0.5290467119834723
1116	2	-0.26394645260609906
1116	3	-0.7150728443844088
1116	4	-1.0002252342145468
1116	5	-0.25883814324053844
1116	6	0.963793145638185
1116	7	-0.8814350953017538
1116	8	0.015750356566179846
1116	9	0.03473051028078089
1116	10-14	0.1882681146948073
1116	15-19	0.21828786960418967
1116	20-24	0.03625624389768234
1116	25-29	0.0331886420659373
1116	30-34	0.2078043735920474
1116	35-39	0.10663841352550207
1116	40-44	-0.0024869153122963894
1116	45-49	0.21115288867416382
1116	50-54	0.02455599228358807
1116	55-59	0.230752721230985
1116	60-64	0.25955382712713515
1116	65-69	0.2555547508722782
1116	70-74	0.2969348008964019
1116	75-79	0.19578158997303063
1116	80-84	0.2524437597576963
1116	85-89	0.2559121790631167
1116	90-94	0.324699154571249
1116	95-99	-0.2808283973398886
1116	100-104	0.5389873465844204
1116	105-109	0.30088537753189826
1116	110-114	0.4080117469669631
1116	115-119	0.8341195384538906
1116	120-124	0.5200628165655132
1116	125-129	0.33106682363549567
1116	130-134	0.353256154076071
1116	135-139	0.5252875859938158
1116	140-144	0.4365011510182413
1116	145-149	0.5070170302613946
1116	150-151	0.5270554460006309
2118	1	0.798377141482149
2118	2	0.37334210633374454
2118	3	0.16459874839063104
2118	4	0.11007148865138561
2118	5	0.14927860321520825
2118	6	1.2114510470697581
2118	7	0.5828022829909898
2118	8	0.24818033875818202
2118	9	0.03975097791416715
2118	10-14	0.19808036752212388
2118	15-19	0.18072918415818862
2118	20-24	0.07635541362871834
2118	25-29	0.20155460797634817
2118	30-34	0.2718696850007305
2118	35-39	-0.014310914627323257
2118	40-44	-0.059666406049828424
2118	45-49	0.14177903636491607
2118	50-54	-0.00452960174032313
2118	55-59	0.181731235997745
2118	60-64	0.08798408684636883
2118	65-69	0.24628774654747332
2118	70-74	0.2814944808149846
2118	75-79	0.19389199955697478
2118	80-84	0.16534368806316024
2118	85-89	0.13573826199750272
2118	90-94	0.18239429090650106
2118	95-99	0.24575695384770313
2118	100-104	0.6128913684165695
2118	105-109	0.23599636529757362
2118	110-114	0.3733572914985146
2118	115-119	0.7212529134163077
2118	120-124	0.410497470465927
2118	125-129	0.30025390011736874
2118	130-134	0.32011292689690407
2118	135-139	0.31940881913982366
2118	140-144	0.2776256913637596
2118	145-149	0.24450180095450946
2118	150-151	0.8185395228756747
1119	1	1.1865411117782259
1119	2	0.29626249612061883
1119	3	0.12693289755976878
1119	4	0.011290162280168659
1119	5	0.13618398501003526
1119	6	1.2634869777122262
1119	7	0.3408737052123243
1119	8	0.022049704637865375
1119	9	0.018929384693890938
1119	10-14	0.1978426925997283
1119	15-19	0.11759655610855191
1119	20-24	-0.045869767938590655
1119	25-29	0.1416451784505881
1119	30-34	0.20265631642134707
1119	35-39	0.05010849633801939
1119	40-44	0.10708940383201337
1119	45-49	0.0676723584253196
1119	50-54	-0.057789540089984825
1119	55-59	0.15732942386253512
1119	60-64	0.2560725091747358
1119	65-69	0.15888930721899186
1119	70-74	0.15898419819954057
1119	75-79	0.3330570892837912
1119	80-84	0.11472934374658905
1119	85-89	0.29153772967825375
1119	90-94	0.24140821212542107
1119	95-99	0.16218838281827175
1119	100-104	0.6139467902169855
1119	105-109	0.23399699304966504
1119	110-114	0.09243130274361988
1119	115-119	0.6155029946067856
1119	120-124	0.32958810081247236
1119	125-129	0.1167119631937581
1119	130-134	0.41684828947816754
1119	135-139	0.2251459651901513
1119	140-144	0.37693944584737693
1119	145-149	0.400349202768453
1119	150-151	0.5939880662549726
2119	1	0.6993670811199735
2119	2	0.3463569956697654
2119	3	0.20345589124777064
2119	4	0.22787591521073836
2119	5	0.17340093721922756
2119	6	1.2651540651784146
2119	7	0.5447258242384763
2119	8	0.3282423105891681
2119	9	0.10772361372905692
2119	10-14	0.22360052848791412
2119	15-19	0.20911453627086019
2119	20-24	0.11916474964481694
2119	25-29	0.20151323976708824
2119	30-34	0.23794598278744417
2119	35-39	0.16407105720366388
2119	40-44	-0.10279863945023493
2119	45-49	0.21702507258221715
2119	50-54	0.15709760147900198
2119	55-59	0.32625984766776384
2119	60-64	0.16292026390873815
2119	65-69	0.22898279684929435
2119	70-74	0.2762083641147868
2119	75-79	0.08230664744429816
2119	80-84	0.23991318504504733
2119	85-89	0.3426496503274876
2119	90-94	0.2763598442264197
2119	95-99	0.3020948210509147
2119	100-104	0.7212153925614331
2119	105-109	0.3608789407502968
2119	110-114	0.39702232167960005
2119	115-119	0.877348044201014
2119	120-124	0.5004618970252892
2119	125-129	0.4473077431757275
2119	130-134	0.4384635103979093
2119	135-139	0.27151747105128266
2119	140-144	0.300065288949277
2119	145-149	0.35197978888207615
2119	150-151	0.20875199772476805
1118	1	0.1926145597856106
1118	2	0.2763408591298102
1118	3	0.13274681444404735
1118	4	0.17879476703385677
1118	5	0.1870195563037953
1118	6	1.203924584961186
1118	7	0.31508113843553076
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2104	150-151	0.04321641124365527
2111	1	1.123125632427822
2111	2	0.3918531727321408
2111	3	0.023753677968095133
2111	4	0.13864291722281052
2111	5	0.09173333158543073
2111	6	1.0368031597458156
2111	7	0.5751564077394846
2111	8	-0.04155809182531556
2111	9	-0.027049826914804953
2111	10-14	-0.06646691618009726
2111	15-19	-0.06900924642531692
2111	20-24	-0.026984626612730267
2111	25-29	0.006625030511557384
2111	30-34	0.0381473509967023
2111	35-39	-0.08932097498949787
2111	40-44	-0.0784994040377569
2111	45-49	-0.09926724130108511
2111	50-54	-0.25555575868197167
2111	55-59	-0.15959673180910272
2111	60-64	-0.12183482663451883
2111	65-69	-0.20699193152093187
2111	70-74	-0.12832443266589877
2111	75-79	-0.13552449943698974
2111	80-84	-0.04447522541772031
2111	85-89	-0.1795132470568177
2111	90-94	-0.5390544012464176
2111	95-99	-0.2903396256291657
2111	100-104	-0.23330581468202638
2111	105-109	-0.19370182383723744
2111	110-114	-0.38414773868256447
2111	115-119	-1.9229321972476683
2111	120-124	-0.8264441794334729
2111	125-129	-0.5617984137659278
2111	130-134	-0.292925302479361
2111	135-139	-0.32992719695675987
2111	140-144	-0.720281753304242
2111	145-149	-1.0713935712788896
2111	150-151	-2.0279393503637593
2110	1	1.3328352094187963
2110	2	0.41873880646959805
2110	3	0.16356632567732987
2110	4	0.09623352787821915
2110	5	0.11177073015467442
2110	6	1.1713734724053708
2110	7	0.42998607596401683
2110	8	-0.032924234274815944
2110	9	-0.014099203972932628
2110	10-14	-0.07843360874310434
2110	15-19	-0.1393247978744938
2110	20-24	-0.09581168559675746
2110	25-29	-0.15691134813384622
2110	30-34	-0.00803277482521736
2110	35-39	-0.16929287296279938
2110	40-44	-0.14383279292160722
2110	45-49	-0.062533644429557
2110	50-54	-0.20775862263793243
2110	55-59	-0.2789284917557069
2110	60-64	-0.04121579780461815
2110	65-69	-0.09256805982955285
2110	70-74	-0.014774664711843855
2110	75-79	-0.12404021410665678
2110	80-84	-0.04551359530411503
2110	85-89	-0.1270124852795007
2110	90-94	-0.18454860887954538
2110	95-99	-0.3578262832933845
2110	100-104	0.20305454806285894
2110	105-109	-0.003574392583033159
2110	110-114	-0.3346837603341939
2110	115-119	-6.652140839274416
2110	120-124	-2.5177375670429285
2110	125-129	-0.24217298764035888
2110	130-134	-0.46881337145348567
2110	135-139	-0.4604687246450183
2110	140-144	-0.37641620516359353
2110	145-149	-0.4152813723034754
2110	150-151	-0.6247731342281355
2109	1	1.19371299306966
2109	2	0.44760039189618084
2109	3	-1.266221134699208E-5
2109	4	0.10444132401577377
2109	5	0.1096047108041347
2109	6	1.1201301658073461
2109	7	0.6777635600875342
2109	8	0.12981400870237536
2109	9	-0.023457518346418738
2109	10-14	0.021393358676590424
2109	15-19	0.0087752909878418
2109	20-24	-0.2100760050721675
2109	25-29	-0.020025628157441133
2109	30-34	-0.033165589539599694
2109	35-39	-0.23726843965167888
2109	40-44	-0.04969272750054898
2109	45-49	-0.2668847387385398
2109	50-54	-0.07710265009333028
2109	55-59	-0.3631856869234298
2109	60-64	-0.12633231470761075
2109	65-69	-0.2528705364840391
2109	70-74	-0.30034257928144115
2109	75-79	-0.18882391859343528
2109	80-84	-0.2931835445146902
2109	85-89	-0.3238573936976721
2109	90-94	-0.44084682244024265
2109	95-99	-0.1281834179427932
2109	100-104	-0.018057437627241768
2109	105-109	-0.17841715987864148
2109	110-114	-0.7209930242323423
2109	115-119	-4.985046043849298
2109	120-124	-1.048230178446417
2109	125-129	-0.45728169078653735
2109	130-134	-0.5482332191618369
2109	135-139	-0.6562403905839318
2109	140-144	-0.35094766702557223
2109	145-149	-0.22617945640094206
2109	150-151	-1.1139332224010232
2108	1	0.8238840151663211
2108	2	0.16486109549150996
2108	3	0.015031648823530475
2108	4	0.09868803642286395
2108	5	-0.02632170092693542
2108	6	0.8052695731998014
2108	7	0.18324988840959833
2108	8	-0.19220403522188434
2108	9	-0.2884282579287003
2108	10-14	-0.16845251964042518
2108	15-19	-0.36442157781113593
2108	20-24	-0.49028822718228326
2108	25-29	-0.36049160871775854
2108	30-34	-5.8320628585672765
2108	35-39	-0.8094546468961497
2108	40-44	-0.7260725075429164
2108	45-49	-0.2678209880238427
2108	50-54	-0.15291908301298207
2108	55-59	-0.27945380652118956
2108	60-64	-0.39589727619821247
2108	65-69	-0.35585627623449767
2108	70-74	-0.28253744693690663
2108	75-79	-0.40912123134358325
2108	80-84	-0.2580823942775865
2108	85-89	-0.4947344494942598
2108	90-94	-0.559585681620284
2108	95-99	-0.611461257380455
2108	100-104	-0.39911173756334506
2108	105-109	-0.4745191341160151
2108	110-114	-0.6772125446305566
2108	115-119	-0.5813233283479988
2108	120-124	-0.7172317928142888
2108	125-129	-0.5143907951486995
2108	130-134	-0.7446828710637661
2108	135-139	-1.2572775378613699
2108	140-144	-1.2228513598920827
2108	145-149	-1.0243540435599954
2108	150-151	-1.4443100343607895
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	3.0
16	0.0
17	1.0
18	2.0
19	24.0
20	68.0
21	192.0
22	447.0
23	814.0
24	1127.0
25	1652.0
26	2279.0
27	3323.0
28	4582.0
29	6504.0
30	8991.0
31	12593.0
32	17837.0
33	25568.0
34	38670.0
35	66937.0
36	142371.0
37	583117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.1446947486611002	21.23270123597608	77.36045143870469	1.2621525766581416
2	49.32973649604951	0.05157550632317889	29.255960623791026	21.36272737383628
3	0.19299925199159962	0.044378924045526014	22.027756999766655	77.73486482419621
4	0.6899995856513235	0.25198941884326936	21.7896155498516	77.26839544565381
5	48.61945563307026	29.257378132421476	21.897019088389296	0.2261471461189704
6	51.31479377430209	27.668459996816058	0.40082782504018094	20.615918403841665
7	21.660731303606358	50.163449649003056	1.0347813002261472	27.141037747164436
8	48.26780445359404	22.41550012975656	28.92164666525643	0.39504875139297485
9	27.170042154525888	43.28428026544485	0.5739819562055257	28.97169562382374
10-14	14.504013730206673	41.75675115745032	24.548043729050857	19.191191383292153
15-19	31.180283109185236	23.07625542197051	24.045089859143257	21.69837160970099
20-24	25.742043960213806	25.254355567864863	17.754818984147892	31.248781487773442
25-29	29.160442349923997	36.56552924320305	14.440552959212823	19.833475447660128
30-34	18.176974862120026	15.622362616153929	37.51938170454323	28.68128081718282
35-39	34.31197402251876	15.304644412508095	19.622681010400154	30.76070055457299
40-44	12.829041916820593	31.594239244925866	27.104291561898243	28.4724272763553
45-49	10.549382729511002	20.927007028662025	37.41243613033229	31.111174111494687
50-54	19.557322958624013	34.396195842992384	16.359816029187595	29.686665169196008
55-59	20.421174525843362	29.469786348737653	43.94477386375779	6.164265261661189
60-64	23.929595617499473	28.721625293587845	20.640844747912446	26.70793434100024
65-69	18.937391914966927	39.54299521754396	21.461255127564876	20.05835773992424
70-74	15.691035457342803	14.231415916659214	35.57510505919734	34.502443566800636
75-79	25.170526288242744	29.894384703119176	24.431197402251875	20.503891606386205
80-84	4.713456082311454	29.93738973418442	32.38821854057673	32.960935642927396
85-89	25.673981738127278	34.637739313620514	28.59254477691685	11.095734171335359
90-94	11.713026468157304	49.94382304258414	23.765731619819825	14.577418869438732
95-99	10.031097958569495	44.45551312722031	33.59611035631806	11.917278557892143
100-104	18.9385041140462	39.375402081774986	15.856578657553905	25.82951514662491
105-109	15.278845755433965	36.75229145722068	31.77969298943847	16.189169797906885
110-114	36.85646743764598	11.754330488866014	25.835664953298544	25.553537120189468
115-119	14.274726257275635	45.190131522993084	16.290249067170283	24.244893152561
120-124	4.629844880940179	20.21328052931953	47.6978787528541	27.458995836886192
125-129	21.253099437140037	34.616956456315656	8.937675416693018	35.19226868985129
130-134	17.447415881766695	55.485605745053434	21.007259824970394	6.059718548209469
135-139	32.04956482485045	13.513764008801637	38.0001570163406	16.436514150007305
140-144	14.124165032897103	38.27532815324795	26.4471563686482	21.153350445206748
145-149	25.306956041966977	33.354326999614	29.83482753281532	11.503889425603694
150-151	38.35347649443573	32.626414510054495	15.054704929222703	13.965404066287066
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	0.5
21	0.5
22	0.5
23	0.0
24	0.0
25	0.0
26	0.0
27	0.5
28	2.0
29	5.0
30	5.0
31	8.0
32	28.0
33	105.0
34	492.0
35	4575.0
36	111530.5
37	119493.0
38	13086.0
39	1255.0
40	1193.5
41	7277.0
42	80223.0
43	89378.0
44	19752.5
45	6015.0
46	9678.0
47	86745.5
48	89387.5
49	19356.5
50	120007.5
51	122784.0
52	12697.5
53	1289.0
54	290.0
55	137.0
56	65.5
57	28.5
58	81.5
59	80.5
60	17.5
61	7.0
62	2.5
63	5.5
64	7.5
65	3.0
66	0.5
67	0.5
68	0.5
69	0.5
70	1.0
71	0.5
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.5
80	0.5
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.109907076857318
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	917102.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	1.1654084455717995
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.36753505791762	0.8433773654171047
2	9.264645870956228	0.21594193086488575
3	3.782647974303062	0.13224989687633543
4	2.059091038850614	0.09598728347510864
5	1.3179606408754923	0.07679812309037601
6	1.061027232141754	0.07419180583918022
7	0.8068199127338832	0.06581923182488988
8	0.33764398471142537	0.031479452110336856
9	0.36574728420105845	0.038362047655757646
>10	4.7826414252332645	1.2399899911566887
>50	1.4723007634011258	1.2912453539119237
>100	1.9505640687097716	4.570014026168515
>500	0.18755423737593957	1.425670388639953
>1k	0.16879881363834562	5.120719513968954
>5k	0.03751084747518792	2.4618360425069823
>10k+	0.03751084747518792	82.31631754649301
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	223874	24.411025164049363	No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	214508	23.389764715375172	No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	160877	17.541887380029703	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	153942	16.785701045249056	No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC	5976	0.6516178135038414	No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	5821	0.6347167490638991	No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	5464	0.5957897812893223	No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG	5265	0.5740909953309447	No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	4415	0.4814077387248092	No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG	4363	0.47573770420302214	No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC	4241	0.4624349309019062	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG	4234	0.4616716570239734	No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	3963	0.43212205403542897	No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	3865	0.4214362197443687	No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	3629	0.3957029861454887	No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCCTCCGCGGCTATATTGA	3326	0.3626641311435369	No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA	2012	0.21938672034299347	No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	1942	0.21175398156366465	No Hit
AGTTTAGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	1813	0.1976879343846159	No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	1697	0.18503939583601386	No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC	1693	0.18460323933433795	No Hit
AGTTTGTAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	1493	0.16279541425054136	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA	1077	0.11743513807624452	No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA	1066	0.1162357076966357	No Hit
TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	1017	0.11089279055110554	No Hit
TTCCATATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	1009	0.11002047754775368	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10-11	0.0	0.0	0.0
12-13	0.0	0.0	0.0
14-15	0.0	0.0	0.0
16-17	0.0	0.0	0.0
18-19	0.0	0.0	0.0
20-21	0.0	0.0	4.3615650167593136E-4
22-23	0.0	0.0	0.001690106443994234
24-25	0.0	0.0	0.0023988607592176224
26-27	0.0	0.0	0.002453380321927114
28-29	0.0	0.0	0.0029440563863125367
30-31	0.0	0.0	0.0029985759490220283
32-33	0.0	0.0	0.0037073302642454168
34-35	0.0	0.0	0.004797721518435245
36-37	0.0	0.0	0.005615514959077616
38-39	0.0	0.0	0.006378788837010496
40-41	0.0	0.0	0.0073601409657813414
42-43	0.0	0.0	0.012375940735054554
44-45	0.0	0.0	0.019408964324578948
46-47	0.0	0.0	0.03783657652038705
48-49	0.0	0.0	0.04230718066256534
50-51	0.0	0.0	0.07600027041703103
52-53	0.0	0.0	0.08030731587108086
54-55	0.0	0.0	0.08123414843714222
56-57	0.0	0.0	0.08128866799985171
58-59	0.0	0.0	0.08227002012862256
60-61	0.0	0.0	0.08870332852834253
62-63	0.0	0.0	0.09202902185362152
64-65	0.0	0.0	0.09235613922987847
66-67	0.0	0.0	0.09311941310781134
68-69	0.0	0.0	0.10146090620236353
70-71	0.0	0.0	0.10151542576507303
72-73	0.0	0.0	0.10151542576507303
74-75	0.0	0.0	0.10162446489049201
76-77	0.0	0.0	0.10238773876842489
78-79	0.0	0.0	0.10238773876842489
80-81	0.0	0.0	0.10315101264635776
82-83	0.0	0.0	0.10326005177177675
84-85	0.0	0.0	0.10326005177177675
86-87	0.0	0.0	0.10331457133448624
88-89	0.0	0.0	0.10347813002261472
90-91	0.0	0.0	0.1035871691480337
92-93	0.0	0.0	0.1035871691480337
94-95	0.0	0.0	0.10369620827345268
96-97	0.0	0.0	0.10369620827345268
98-99	0.0	0.0	0.10369620827345268
100-101	0.0	0.0	0.10369620827345268
102-103	0.0	0.0	0.10478659952764251
104-105	0.0	0.0	0.10778517547666454
106-107	0.0	0.0	0.10952980148336826
108-109	0.0	0.0	0.10963884060878724
110-111	0.0	0.0	0.10985691885962522
112-113	0.0	0.0	0.10991143842233471
114-115	0.0	0.0	0.11023855579859165
116-117	0.0	0.0	0.11023855579859165
118-119	0.0	0.0	0.1112199079273625
120-121	0.0	0.0	0.11241933830697132
122-123	0.0	0.0	0.11639926638476418
124-125	0.0	0.0	0.12255997697093671
126-127	0.0	0.0	0.12501335729286384
128-129	0.0	0.0	0.12506787685557333
130-131	0.0	0.0	0.1251769159809923
132-133	0.0	0.0	0.12528595510641127
134-135	0.0	0.0	0.12528595510641127
136-137	0.0	0.0	0.12528595510641127
138-139	0.0	0.0	0.12528595510641127
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCCACA	35	9.094947E-12	148.10378	1
TGCCGAG	35	9.094947E-12	148.10378	1
TGCCAGA	35	9.094947E-12	148.10378	1
CGTTTGT	85	0.0	148.10376	1
AGCTTGG	40	0.0	148.10376	1
AATTTGG	15	1.1304478E-4	148.10376	1
TGCCGGG	10	0.006655666	148.10376	1
TTCCGCA	30	5.620677E-10	148.10376	1
AGGGTGT	10	0.006655666	148.10376	1
AGTGGAA	15	1.1304478E-4	148.10376	1
GGTTTGG	10	0.006655666	148.10376	1
ACCCGCA	25	3.270361E-8	148.10376	1
TACACGC	10	0.006655666	148.10376	1
CGGGTGT	45	0.0	148.10376	1
CCCCGCA	30	5.620677E-10	148.10376	1
TCTCGCA	30	5.620677E-10	148.10376	1
AGTGTGG	20	1.917333E-6	148.10376	1
CGTGTGT	105	0.0	148.10376	1
CGTGTGG	150	0.0	148.10376	1
TCCCGGA	390	0.0	148.10376	1
>>END_MODULE