##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AG1W8 l01n01 mis20.3410000000d2b2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	744609
Sequences flagged as poor quality	0
Sequence length	151
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12513413079885	34.0	34.0	34.0	31.0	34.0
2	33.44602603514059	34.0	34.0	34.0	33.0	34.0
3	33.46759574488087	34.0	34.0	34.0	33.0	34.0
4	33.49804931178645	34.0	34.0	34.0	34.0	34.0
5	33.675819121176346	34.0	34.0	34.0	34.0	34.0
6	36.4042833218508	38.0	38.0	38.0	34.0	38.0
7	36.722649068168664	38.0	38.0	38.0	34.0	38.0
8	36.98444687077379	38.0	37.0	38.0	35.0	38.0
9	37.41464043545002	38.0	38.0	38.0	37.0	38.0
10-14	37.29769906084938	38.0	38.0	38.0	36.4	38.0
15-19	37.32691922874959	38.0	38.0	38.0	36.8	38.0
20-24	37.54238667542294	38.0	38.0	38.0	37.4	38.0
25-29	37.253343432593475	38.0	38.0	38.0	36.6	38.0
30-34	36.840057802148515	38.0	37.8	38.0	35.4	38.0
35-39	37.14635761856223	38.0	38.0	38.0	36.0	38.0
40-44	36.89320784465404	38.0	38.0	38.0	35.4	38.0
45-49	37.123728292298374	38.0	38.0	38.0	36.4	38.0
50-54	37.06548349536468	38.0	37.8	38.0	36.0	38.0
55-59	36.896215329119045	38.0	37.8	38.0	35.6	38.0
60-64	36.63554697834702	38.0	37.2	38.0	35.0	38.0
65-69	36.81755659681793	38.0	37.6	38.0	35.2	38.0
70-74	36.88636479011132	38.0	38.0	38.0	35.6	38.0
75-79	36.49265963747416	38.0	37.4	38.0	34.2	38.0
80-84	36.722538137465435	38.0	37.6	38.0	34.4	38.0
85-89	36.40973557934433	38.0	37.0	38.0	33.2	38.0
90-94	36.696122125840546	38.0	37.6	38.0	35.0	38.0
95-99	36.551417455335624	38.0	37.0	38.0	34.4	38.0
100-104	36.092141513196864	38.0	37.0	38.0	32.6	38.0
105-109	36.21620689516242	38.0	37.0	38.0	33.2	38.0
110-114	36.41641425231229	38.0	37.0	38.0	34.0	38.0
115-119	35.94641321821251	38.0	37.0	38.0	31.2	38.0
120-124	36.09775868945984	38.0	36.8	38.0	32.8	38.0
125-129	36.02087162524224	38.0	37.0	38.0	32.4	38.0
130-134	35.56815939640804	38.0	36.8	38.0	30.2	38.0
135-139	35.42404859463154	38.0	36.6	38.0	29.0	38.0
140-144	35.57266807143078	38.0	37.0	38.0	30.2	38.0
145-149	35.31069192018899	38.0	36.2	38.0	28.8	38.0
150-151	33.78692911313186	36.5	33.0	37.5	24.5	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.44999296636958874
1101	2	0.237827376280066
1101	3	0.1448780882449654
1101	4	0.0674371086837553
1101	5	0.16107680006192027
1101	6	1.1024345098510224
1101	7	-0.362950306668246
1101	8	0.22835946367445814
1101	9	0.1751908393745154
1101	10-14	0.2601238940981574
1101	15-19	0.24913837494346325
1101	20-24	0.14821949725934047
1101	25-29	0.2362275711860633
1101	30-34	0.26103972447052115
1101	35-39	0.08120212574521446
1101	40-44	0.1279881738903157
1101	45-49	0.27224930561624205
1101	50-54	0.27138893114423723
1101	55-59	0.33131018013165203
1101	60-64	0.44191251000971477
1101	65-69	0.29681258979098857
1101	70-74	0.10333564410130691
1101	75-79	0.15523746280253192
1101	80-84	0.25619124729536935
1101	85-89	0.2826285892007192
1101	90-94	0.3657561566095069
1101	95-99	0.4139031888161355
1101	100-104	0.6691880971325119
1101	105-109	0.40608257745120113
1101	110-114	0.34282255693791086
1101	115-119	0.69040254582616
1101	120-124	0.4228810051406455
1101	125-129	0.35073363504453425
1101	130-134	0.4157980699611272
1101	135-139	0.43549619013940344
1101	140-144	0.2848992164001274
1101	145-149	0.46781549464276395
1101	150-151	0.3150938793479625
1102	1	0.32847569540791355
1102	2	0.25633131422613786
1102	3	0.12573740646198672
1102	4	0.2167714111132497
1102	5	0.16182077994920263
1102	6	1.12029908263451
1102	7	0.39327093321880113
1102	8	0.2428496754060845
1102	9	0.07981747919244953
1102	10-14	0.21677770038219535
1102	15-19	0.17240802594970006
1102	20-24	0.06598960699756873
1102	25-29	0.20392597575192895
1102	30-34	0.12488211130670379
1102	35-39	0.17632312375539527
1102	40-44	0.18803130661280676
1102	45-49	0.17924772090572105
1102	50-54	0.15034764526484423
1102	55-59	0.28534051601879185
1102	60-64	0.34031964954764504
1102	65-69	0.26465330851083735
1102	70-74	0.12736609913960706
1102	75-79	0.0769738289302424
1102	80-84	0.24747929524178858
1102	85-89	0.1438455515608581
1102	90-94	0.2297583489909769
1102	95-99	0.2724810958067323
1102	100-104	0.6378529129843855
1102	105-109	0.2515572399555239
1102	110-114	0.23755807709233068
1102	115-119	0.5643453770961955
1102	120-124	0.31322661501589266
1102	125-129	0.2337475399857354
1102	130-134	0.23278440332226324
1102	135-139	0.42242068404482325
1102	140-144	0.2486244061483589
1102	145-149	0.43556518187094184
1102	150-151	0.9051502378500018
1103	1	1.0046624398500335
1103	2	0.3376681621879243
1103	3	0.1828818629255693
1103	4	0.19845046524589094
1103	5	0.13904960300423141
1103	6	1.1390314901065395
1103	7	0.48286415326207077
1103	8	0.28316927784403134
1103	9	0.09139534963389906
1103	10-14	0.1793354697853431
1103	15-19	0.24112269550095533
1103	20-24	0.10026382555976454
1103	25-29	0.18005587161191272
1103	30-34	0.31383443991767734
1103	35-39	0.0896728961672082
1103	40-44	0.3236366446607377
1103	45-49	0.1972948921435389
1103	50-54	0.10415490094678859
1103	55-59	0.2599482947270815
1103	60-64	0.45010366370696175
1103	65-69	0.2528605959853394
1103	70-74	0.24435277534404065
1103	75-79	0.28043713372781554
1103	80-84	0.36046796041963347
1103	85-89	0.3909252403815131
1103	90-94	0.32250625339619177
1103	95-99	0.44622877921489845
1103	100-104	0.5923914459517192
1103	105-109	0.41281960803579665
1103	110-114	0.4234447017307872
1103	115-119	0.8746408346376242
1103	120-124	0.7720003431197426
1103	125-129	0.39259900476966436
1103	130-134	0.5267462890861765
1103	135-139	0.5824490550251937
1103	140-144	0.678276490887157
1103	145-149	0.744608274356267
1103	150-151	0.8195155789221076
2116	1	0.6741744388502902
2116	2	0.2542039707119912
2116	3	-0.08717323577886305
2116	4	-0.42375772365692654
2116	5	-0.011855443664238408
2116	6	0.8990740202832939
2116	7	-0.13213096068438546
2116	8	0.08427847345826223
2116	9	0.05577811917606823
2116	10-14	0.203808042328383
2116	15-19	0.24969357093424804
2116	20-24	0.09389525937577048
2116	25-29	0.24722148936478305
2116	30-34	0.2603937670558665
2116	35-39	0.10941296221599117
2116	40-44	0.06917507342065221
2116	45-49	0.20863038271561152
2116	50-54	0.16583379811226706
2116	55-59	0.35370065068580914
2116	60-64	0.40722895359231615
2116	65-69	0.2802585522423513
2116	70-74	0.2361369838648244
2116	75-79	0.19474195176584885
2116	80-84	0.3524964716176129
2116	85-89	0.3487715560717888
2116	90-94	0.4001411371552308
2116	95-99	0.419464063691791
2116	100-104	0.6564927296419256
2116	105-109	0.4570186742566662
2116	110-114	0.3953381140604506
2116	115-119	0.8028772049041919
2116	120-124	0.49098173924040367
2116	125-129	0.36371408003742545
2116	130-134	0.5077488109863566
2116	135-139	0.6320013716496575
2116	140-144	0.6132828622190374
2116	145-149	0.610294913407003
2116	150-151	1.2885109059291793
1117	1	0.5552627916746111
1117	2	0.2585127457258096
1117	3	0.19980319331339302
1117	4	0.18491953741084188
1117	5	0.16742568711485006
1117	6	1.172536644745712
1117	7	0.3161207806552966
1117	8	0.25234883110863393
1117	9	0.08371964189375802
1117	10-14	0.21782548778567445
1117	15-19	0.1594523469491662
1117	20-24	0.10953194861654936
1117	25-29	0.2580279711046316
1117	30-34	0.3803507629417382
1117	35-39	0.1515449302041887
1117	40-44	0.10514539936537659
1117	45-49	0.28634435213371745
1117	50-54	0.22438685970968208
1117	55-59	0.4032953328550448
1117	60-64	0.4727605529231269
1117	65-69	0.30496336149015946
1117	70-74	0.2557942333705583
1117	75-79	0.22477214436437976
1117	80-84	0.3075684758835564
1117	85-89	0.3700847478271072
1117	90-94	0.2811811838494691
1117	95-99	-0.18138126824050715
1117	100-104	0.4333411677648371
1117	105-109	0.40607050805216005
1117	110-114	0.3908682003398525
1117	115-119	0.6952614368303927
1117	120-124	0.4633632542635766
1117	125-129	0.3130774452867584
1117	130-134	0.46600643472519465
1117	135-139	0.19750694244132916
1117	140-144	0.3232057168730904
1117	145-149	-0.5360485330402298
1117	150-151	0.10007011173178881
2117	1	-29.96805581411822
2117	2	-10.423877754727709
2117	3	-2.1304774596767153
2117	4	-0.696666448356936
2117	5	-0.10627525015512873
2117	6	1.1556920595840126
2117	7	-0.15603172198944293
2117	8	0.09814054483994283
2117	9	0.10415308290219372
2117	10-14	0.3374023051409125
2117	15-19	0.33421991518269323
2117	20-24	0.22440437593271412
2117	25-29	0.3037848617141492
2117	30-34	0.43313928413449077
2117	35-39	0.22635561876542454
2117	40-44	0.1769098190606755
2117	45-49	0.33101349161918137
2117	50-54	0.2195967947337607
2117	55-59	0.46900706654993485
2117	60-64	0.35711403400959085
2117	65-69	0.372855311754698
2117	70-74	0.42806603977913227
2117	75-79	0.41619321059304326
2117	80-84	0.5075180640718528
2117	85-89	0.33910052948990455
2117	90-94	0.4100357231439702
2117	95-99	0.05538421689146844
2117	100-104	0.8017865793785433
2117	105-109	0.6545412764300025
2117	110-114	0.5296175976924147
2117	115-119	0.9542856225570588
2117	120-124	0.8116736099937114
2117	125-129	0.5038232949552182
2117	130-134	0.6961352237039833
2117	135-139	0.5597826271651059
2117	140-144	0.7799453423799534
2117	145-149	0.8602187300195396
2117	150-151	1.1615596361950367
1116	1	-0.5912029064785393
1116	2	-0.3572395091664404
1116	3	-0.6809616647933865
1116	4	-1.1734511745434304
1116	5	-0.3144484106063814
1116	6	0.9215829371391706
1116	7	-0.9044698995152558
1116	8	0.04721031330040404
1116	9	-0.021096392597009128
1116	10-14	0.17027613149283383
1116	15-19	0.12712408009127785
1116	20-24	0.04159135809311465
1116	25-29	-5.501355191768198E-4
1116	30-34	0.07846053265807029
1116	35-39	0.08640055720501749
1116	40-44	-0.03387905641253752
1116	45-49	0.1470162772768404
1116	50-54	0.02297243366113122
1116	55-59	0.23032745076045558
1116	60-64	0.1736241655983335
1116	65-69	0.1795388461527594
1116	70-74	0.17474215743666832
1116	75-79	0.10949916427929907
1116	80-84	0.3212470639652736
1116	85-89	0.2284081592427114
1116	90-94	0.2746304230944716
1116	95-99	-0.3743414367087965
1116	100-104	0.5438053343881251
1116	105-109	0.29624671795235713
1116	110-114	0.21207160441128536
1116	115-119	0.6042468598679349
1116	120-124	0.3656953667392244
1116	125-129	0.055431691055886745
1116	130-134	0.16342696002426038
1116	135-139	0.1590602249587718
1116	140-144	0.14690171938981678
1116	145-149	0.22652862421418263
1116	150-151	0.20766862140097686
2118	1	0.7928137510991711
2118	2	0.4019934805893328
2118	3	0.19416903589752366
2118	4	0.19521547559786256
2118	5	0.15952806777617923
2118	6	1.2138950323613855
2118	7	0.6709111606025502
2118	8	0.3275356904169655
2118	9	0.16251610689596419
2118	10-14	0.22518135095731395
2118	15-19	0.21446402808143006
2118	20-24	0.0823039472738003
2118	25-29	0.20500960145695046
2118	30-34	0.2791588799703746
2118	35-39	0.039787395194750275
2118	40-44	-0.012259584713753213
2118	45-49	0.14370346545428703
2118	50-54	-0.01561029762275723
2118	55-59	0.26316171777746433
2118	60-64	0.15133688097278508
2118	65-69	0.17082753243052906
2118	70-74	0.28580416815412946
2118	75-79	0.23581270622295847
2118	80-84	0.13865707203266453
2118	85-89	0.1416683077006624
2118	90-94	0.21212056420614545
2118	95-99	0.343651766855281
2118	100-104	0.5785554932570633
2118	105-109	0.2901491927023372
2118	110-114	0.3902227304653394
2118	115-119	0.7341748392803282
2118	120-124	0.42383193544046094
2118	125-129	0.2589296246328914
2118	130-134	0.38355937334491585
2118	135-139	0.38837751666017795
2118	140-144	0.3077552850398675
2118	145-149	0.3309864191583216
2118	150-151	0.9129165921600801
1119	1	1.052078414069701
1119	2	0.25179474682625624
1119	3	0.03853662483032849
1119	4	-0.13090795597494775
1119	5	0.15679022833713674
1119	6	1.245055779016731
1119	7	0.29757132807830544
1119	8	0.02520946640267141
1119	9	0.020069979441082353
1119	10-14	0.2351107441008935
1119	15-19	0.14385120301134435
1119	20-24	-0.026247573183177053
1119	25-29	0.10688419802077931
1119	30-34	0.2737477508796431
1119	35-39	0.15348455058289545
1119	40-44	0.15061544512947478
1119	45-49	0.12921203819658444
1119	50-54	0.06245228030122263
1119	55-59	0.24067363296080657
1119	60-64	0.3025998019833125
1119	65-69	0.23971475599385883
1119	70-74	0.2676289817997741
1119	75-79	0.3393384067649947
1119	80-84	0.20098210231801517
1119	85-89	0.3916729499798919
1119	90-94	0.3008552999499301
1119	95-99	0.3465936455193628
1119	100-104	0.6492220361296219
1119	105-109	0.30751772777586694
1119	110-114	0.3209705353304457
1119	115-119	0.7733211904488542
1119	120-124	0.5932791063028588
1119	125-129	0.3245814006362906
1119	130-134	0.42008747902971777
1119	135-139	0.3796093565059593
1119	140-144	0.5185143197994861
1119	145-149	0.4422945692321605
1119	150-151	0.8246922860702952
2119	1	0.837855515931043
2119	2	0.3292150970538259
2119	3	0.22031002384510856
2119	4	0.2143576902048423
2119	5	0.14473173217773905
2119	6	1.200197335755142
2119	7	0.5984121828187199
2119	8	0.25910318589000525
2119	9	0.10465137043302519
2119	10-14	0.1582202535839201
2119	15-19	0.13936161175711703
2119	20-24	0.06606979162435778
2119	25-29	0.12177316772576319
2119	30-34	0.16434141857119755
2119	35-39	0.09694164274629458
2119	40-44	-0.11914386984391001
2119	45-49	0.0925309348364749
2119	50-54	0.09378133772840869
2119	55-59	0.22799358755685262
2119	60-64	0.15264950388210963
2119	65-69	0.22738714223601875
2119	70-74	0.17177683117327547
2119	75-79	-0.039789330421058366
2119	80-84	0.13854472560847597
2119	85-89	0.24640895685661235
2119	90-94	0.21354757797200108
2119	95-99	0.2155747014918603
2119	100-104	0.6830037885899216
2119	105-109	0.3392734941879567
2119	110-114	0.3536061686163947
2119	115-119	0.785019871309764
2119	120-124	0.3875360574054554
2119	125-129	0.3676448449448131
2119	130-134	0.3848654735415735
2119	135-139	0.20611832158240162
2119	140-144	0.31680550019202514
2119	145-149	0.25584048563158035
2119	150-151	0.09159754389200003
1118	1	0.14695862284039762
1118	2	0.30687265903318206
1118	3	0.18531237073985807
1118	4	0.21866507178147998
1118	5	0.16321957336645454
1118	6	1.2532324159051527
1118	7	0.4762257303279256
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2111	1	1.2065314795286035
2111	2	0.4473914265979104
2111	3	0.10331901602592808
2111	4	0.20434775913093262
2111	5	0.1114968881547469
2111	6	0.9541950242545667
2111	7	0.6214133385690062
2111	8	-0.023143848957467128
2111	9	-0.08388748027311976
2111	10-14	-0.11656654868420446
2111	15-19	0.03446132910347899
2111	20-24	-0.03042880250028901
2111	25-29	0.009782739013871833
2111	30-34	0.10118540706791634
2111	35-39	-0.1424518963878043
2111	40-44	-0.10240653565957558
2111	45-49	-0.13183380911184628
2111	50-54	-0.2928510425011268
2111	55-59	-0.11626277707865995
2111	60-64	-0.13293989180095167
2111	65-69	-0.27252749488549455
2111	70-74	-0.1643803114374407
2111	75-79	-0.2562163616706812
2111	80-84	-0.03753922769823248
2111	85-89	-0.25039338520112153
2111	90-94	-0.5513962732639754
2111	95-99	-0.4096823112509682
2111	100-104	-0.2533897283656117
2111	105-109	-0.16776795757240137
2111	110-114	-0.40540087184965046
2111	115-119	-1.830089407467554
2111	120-124	-0.7456348137444024
2111	125-129	-0.4934880367669763
2111	130-134	-0.2738930682909242
2111	135-139	-0.17131448349008593
2111	140-144	-0.6694415149411554
2111	145-149	-0.8601878240897847
2111	150-151	-2.089172644157685
2110	1	1.5587389280455817
2110	2	0.4137937379659249
2110	3	0.183632681453183
2110	4	0.1723388325240478
2110	5	0.1461881468275621
2110	6	1.2084257796727158
2110	7	0.46649209635032207
2110	8	0.04528318559115263
2110	9	-0.048521212710618045
2110	10-14	-0.0354836917855792
2110	15-19	-0.13367688560550306
2110	20-24	-0.165830072397128
2110	25-29	-0.2111499720332617
2110	30-34	-0.005871913301064069
2110	35-39	-0.22956410770073177
2110	40-44	-0.13015185422381137
2110	45-49	-0.13785274375612033
2110	50-54	-0.28761829468130173
2110	55-59	-0.3220758959738035
2110	60-64	-0.24577912233633725
2110	65-69	-0.21881995355163042
2110	70-74	-0.1507754807106565
2110	75-79	-0.2543357449214554
2110	80-84	-0.19810641725863576
2110	85-89	-0.19266618995536788
2110	90-94	-0.2124543048075438
2110	95-99	-0.5472619200644715
2110	100-104	0.113154261526212
2110	105-109	-0.16081367153150694
2110	110-114	-0.5631599333824369
2110	115-119	-6.624024443742009
2110	120-124	-2.7225693882106086
2110	125-129	-0.15436893221976078
2110	130-134	-0.3032592680743207
2110	135-139	-0.461052578017636
2110	140-144	-0.27683429120794045
2110	145-149	-0.4318778146867004
2110	150-151	-0.817722712923107
2109	1	1.301440730797868
2109	2	0.466860193557892
2109	3	0.04149812532391195
2109	4	0.11457360255609927
2109	5	0.11279615575497814
2109	6	1.0543349263872699
2109	7	0.7593986688452006
2109	8	0.07993483747360841
2109	9	0.04743459735009736
2109	10-14	0.03720322860326064
2109	15-19	0.04097638327419162
2109	20-24	-0.23262782896317447
2109	25-29	-0.06711444600509253
2109	30-34	-0.029541259325498004
2109	35-39	-0.17156121906493382
2109	40-44	-0.0020876694150047115
2109	45-49	-0.17177534947729356
2109	50-54	-0.03787666488783259
2109	55-59	-0.32268746029885875
2109	60-64	-0.22111053190876362
2109	65-69	-0.2876101102306805
2109	70-74	-0.3040075287973565
2109	75-79	-0.16699613457786455
2109	80-84	-0.2902386277437614
2109	85-89	-0.30607379203829765
2109	90-94	-0.350778935967476
2109	95-99	-0.1342351108491897
2109	100-104	4.0972812473683007E-4
2109	105-109	-0.2772870804112202
2109	110-114	-0.8858107707773897
2109	115-119	-4.927323118103729
2109	120-124	-1.0809197316228918
2109	125-129	-0.6215093524593769
2109	130-134	-0.5534180232659409
2109	135-139	-0.617872263861031
2109	140-144	-0.5659577888727867
2109	145-149	-0.41477637206564566
2109	150-151	-1.518438733273321
2108	1	0.9339942997769946
2108	2	0.23270264712844835
2108	3	0.07213571366859384
2108	4	0.04827826652585543
2108	5	-0.02841817188096485
2108	6	0.8642457215850072
2108	7	0.1544289921816926
2108	8	-0.17208278311702685
2108	9	-0.23395966898706178
2108	10-14	-0.1285472542989723
2108	15-19	-0.305627223145315
2108	20-24	-0.38401818261176146
2108	25-29	-0.28398532953064404
2108	30-34	-6.210961497819607
2108	35-39	-0.739388462620802
2108	40-44	-0.7784966308210528
2108	45-49	-0.206495513540375
2108	50-54	-0.1504836173306785
2108	55-59	-0.3182287942558162
2108	60-64	-0.4975734614331415
2108	65-69	-0.41067304830001206
2108	70-74	-0.3631487161409339
2108	75-79	-0.45191204550349084
2108	80-84	-0.2972653891844388
2108	85-89	-0.6160673383508168
2108	90-94	-0.7024535217059551
2108	95-99	-0.6472132065391634
2108	100-104	-0.5178426267965932
2108	105-109	-0.5844127834021791
2108	110-114	-0.8679327072434333
2108	115-119	-0.7654199998708222
2108	120-124	-0.98208971545494
2108	125-129	-0.5120867438654031
2108	130-134	-0.748803074546899
2108	135-139	-1.3529007208000934
2108	140-144	-1.3237252627376037
2108	145-149	-1.0848076510875586
2108	150-151	-1.725426705128946
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	0.0
17	1.0
18	3.0
19	19.0
20	77.0
21	233.0
22	540.0
23	950.0
24	1364.0
25	1698.0
26	2268.0
27	3221.0
28	4401.0
29	6111.0
30	8519.0
31	12470.0
32	17997.0
33	23780.0
34	32844.0
35	56505.0
36	120268.0
37	451339.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.1473265175385772	23.22573742134698	75.27177901571072	1.3551570454037283
2	57.92328591247219	0.0664778427335689	18.68578005369261	23.32445619110164
3	0.20158230695573112	0.04270697775611093	24.086063961085618	75.66964675420255
4	0.7425373585331362	0.21017742197582892	23.850906986082627	75.1963782334084
5	57.10809297228479	18.715728657590763	23.938469720349875	0.2377086497745797
6	43.077642091352644	34.019733846891455	0.3706643352417175	22.531959726514184
7	23.697537902442757	41.61888991403542	1.0279220369348208	33.655650146587
8	56.765765656874954	24.515954010762695	18.374072835541874	0.3442074968204789
9	33.67928671289227	47.33947615459926	0.4883099720793061	18.492927160429165
10-14	13.178379525361633	46.96781800918334	21.529366419154215	18.32443604630081
15-19	30.380239830568794	22.59123916041842	27.865953809314686	19.162567199698096
20-24	23.676211273299142	26.60961659072077	19.475939721383977	30.238232414596116
25-29	26.344054396334183	33.3155521891355	16.69662870043204	23.64376471409827
30-34	21.036382853282728	15.667874011729646	30.059803198725778	33.23593993626185
35-39	32.29023554644115	19.001220774930196	23.952893397742976	24.75565028088567
40-44	14.453652856734205	27.110765515861345	29.28984205133164	29.14573957607281
45-49	8.704944474213985	26.029285168457537	35.32748059719933	29.938289760129145
50-54	23.253412193513643	36.39931829993997	12.319056041492917	28.02821346505347
55-59	16.30074307455322	31.36024410126657	48.19318595397048	4.145826870209735
60-64	24.369111842591213	29.696176113906763	25.17595140536846	20.758760638133573
65-69	17.95293905929152	42.981403662861986	18.507646294901082	20.55801098294541
70-74	15.405212668662344	16.983839840775495	34.252634604201674	33.358312886360494
75-79	26.49745033970849	32.183253224175374	20.69717126706768	20.622125169048452
80-84	5.587227659080134	31.82172119864251	31.98091884465538	30.610132297621973
85-89	22.942833084209298	33.06833519336994	29.741273608027836	14.247558114392922
90-94	6.834835463981767	51.22559625252985	28.288538011224684	13.651030272263698
95-99	12.26138819165495	44.44477571450251	36.13754332810912	7.156292765733426
100-104	17.951220036287502	42.78145979970696	15.804214023735947	23.46310614026959
105-109	18.96020596044367	42.01617224610501	27.134724398979866	11.88889739447146
110-114	32.92482363226875	11.19954231012518	29.031773722853206	26.843860334752872
115-119	16.566090391064304	47.06311634696868	11.748071806814046	24.622721455152973
120-124	5.2063566247520505	21.34878842452885	51.81197111504159	21.632883835677514
125-129	17.906659736855183	33.485332570516874	10.254509413665428	38.35349827896252
130-134	9.935066591996605	59.96594185673286	26.041184030813486	4.057807520457045
135-139	31.431341818323443	15.44150016988782	40.80431474774009	12.322843264048648
140-144	16.838931573483535	35.73905230798983	29.167603399905182	18.254412718621452
145-149	30.311076014391446	31.131157426246524	27.745541619830007	10.812224939532022
150-151	36.35512060692256	36.66749931843424	9.297631374318602	17.6797487003246
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	0.5
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	1.0
26	1.0
27	0.0
28	1.0
29	2.0
30	4.0
31	13.0
32	32.0
33	105.5
34	518.0
35	4546.5
36	112926.0
37	120394.0
38	12535.0
39	1270.5
40	1188.0
41	6448.0
42	71623.5
43	79578.0
44	17297.0
45	5460.5
46	9269.5
47	79127.0
48	81388.0
49	17296.0
50	58060.5
51	56621.0
52	6695.0
53	810.0
54	206.5
55	97.0
56	51.5
57	69.5
58	351.0
59	387.5
60	91.0
61	12.5
62	5.0
63	14.0
64	54.0
65	50.0
66	6.5
67	0.5
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.097208064903862
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	744609.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	1.5173257978543218
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.38755848990742	1.0983550994042517
2	9.803965039700428	0.2975161815199866
3	3.696536090944043	0.168265487204669
4	2.183792599757691	0.13254099395102809
5	1.4492436460782647	0.10994873857855046
6	0.9081197431913702	0.08267481083310646
7	0.550998913172544	0.058523140588247625
8	0.6838395777009593	0.08300859462715765
9	0.4084226560836779	0.0557739209253553
>10	4.527490026269787	1.5335489683336294
>50	1.5446626104222476	1.706631835626733
>100	1.482521011152387	4.686839362322981
>500	0.15091531251251847	1.5727413822239724
>1k	0.16867005516105002	6.523185993348966
>5k	0.017754742648531583	1.4566311499974338
>10k+	0.035509485297063166	80.43381434051393
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	216820	29.118638104025063	No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	144245	19.371911969906353	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	136874	18.381996457201026	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	99200	13.322428281151586	No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	5719	0.7680541062490516	No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG	5095	0.684251734803098	No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG	3860	0.5183928746496483	No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	3804	0.5108721490070628	No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	3680	0.49421911365562327	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG	3634	0.488041374734928	No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	3538	0.4751487022047813	No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	3486	0.46816517125095186	No Hit
TTCCAGATCTCGATGCCGACAGGCAGCGCTTTGTTTTTCAGTAAAGAAAC	3290	0.44184263150190234	No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCCTCCGCGGCTATATTGA	3051	0.4097452488487246	No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC	2652	0.35616007864530247	No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	2508	0.3368210698500824	No Hit
TTCCAGATCTCGATGCCGACAGGGAAAGCAATAGAATCCAAAGTACCTGT	2159	0.2899508332561116	No Hit
TTCCAGATCTCGATGCCGACAGGGGAAAGCAATAGAATCCAAAGTACCTG	2122	0.28498178238511757	No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC	1762	0.23663426039706748	No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA	1738	0.23341109226453077	No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	1714	0.23018792413199413	No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC	1569	0.21071461666458502	No Hit
AGTTTAGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	1480	0.19876203483976151	No Hit
AGTTTGTAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	1360	0.18264619417707817	No Hit
TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	1021	0.13711894430499766	No Hit
TTCCAGATCTCGATGCCGACGACGGGAAAGCAATAGAATCCAAAGTACCT	981	0.13174699741743653	No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA	930	0.12489776513579612	No Hit
TTCCAGATCTCGATGCCGACAGGCAATGAATCCAAAGTACCTGTCTCTTA	908	0.12194319434763749	No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	800	0.10743893775122246	No Hit
TCCCGCAGATCATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	794	0.10663314571808828	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10-11	0.0	0.0	0.0
12-13	0.0	0.0	3.3574668047257016E-4
14-15	0.0	0.0	6.714933609451403E-4
16-17	0.0	0.0	6.714933609451403E-4
18-19	0.0	0.0	0.0010743893775122244
20-21	0.0	0.0	0.0016115840662683368
22-23	0.0	0.0	0.0030888694603476456
24-25	0.0	0.0	0.00349176547691473
26-27	0.0	0.0	0.003558914813009244
28-29	0.0	0.0	0.0037603628212927857
30-31	0.0	0.0	0.0037603628212927857
32-33	0.0	0.0	0.004633304190521468
34-35	0.0	0.0	0.00698353095382946
36-37	0.0	0.0	0.035454849457903406
38-39	0.0	0.0	0.05110064476792518
40-41	0.0	0.0	0.17673705260076092
42-43	0.0	0.0	0.3162733730051611
44-45	0.0	0.0	0.3283602535021736
46-47	0.0	0.0	0.6341583300765905
48-49	0.0	0.0	1.3384205670358538
50-51	0.0	0.0	1.5082412380188797
52-53	0.0	0.0	1.5174406970638281
54-55	0.0	0.0	1.5175749957360172
56-57	0.0	0.0	1.5175749957360172
58-59	0.0	0.0	1.5175749957360172
60-61	0.0	0.0	1.5229469426235784
62-63	0.0	0.0	1.5276473961501942
64-65	0.0	0.0	1.5276473961501942
66-67	0.0	0.0	1.5292589802164627
68-69	0.0	0.0	1.5312063109632037
70-71	0.0	0.0	1.5312734602992981
72-73	0.0	0.0	1.5312734602992981
74-75	0.0	0.0	1.5312734602992981
76-77	0.0	0.0	1.5312734602992981
78-79	0.0	0.0	1.5312734602992981
80-81	0.0	0.0	1.5313406096353925
82-83	0.0	0.0	1.531407758971487
84-85	0.0	0.0	1.5314749083075814
86-87	0.0	0.0	1.531542057643676
88-89	0.0	0.0	1.531542057643676
90-91	0.0	0.0	1.531542057643676
92-93	0.0	0.0	1.531542057643676
94-95	0.0	0.0	1.531676356315865
96-97	0.0	0.0	1.5319449536602432
98-99	0.0	0.0	1.5319449536602432
100-101	0.0	0.0	1.5319449536602432
102-103	0.0	0.0	1.5322807003407157
104-105	0.0	0.0	1.532817895029472
106-107	0.0	0.0	1.5328850443655664
108-109	0.0	0.0	1.5328850443655664
110-111	0.0	0.0	1.533220791046039
112-113	0.0	0.0	1.5604834215004115
114-115	0.0	0.0	1.5853958251914764
116-117	0.0	0.0	1.5853958251914764
118-119	0.0	0.0	1.5860673185524214
120-121	0.0	0.0	1.5861344678885159
122-123	0.0	0.0	1.5879474999630678
124-125	0.0	0.0	1.5905663240707537
126-127	0.0	0.0	1.5906334734068484
128-129	0.0	0.0	1.5907677720790374
130-131	0.0	0.0	1.5909020707512265
132-133	0.0	0.0	1.5909020707512265
134-135	0.0	0.0	1.5909020707512265
136-137	0.0	0.0	1.5914392654399827
138-139	0.0	0.0	1.5918421614565497
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTGT	65	0.0	148.08148	1
TTCCGCA	35	9.094947E-12	148.08148	1
TGCCGAG	40	0.0	148.08148	1
AGGGTGT	10	0.006658406	148.08148	1
TTTCAGA	10	0.006658406	148.08148	1
GGTTTGG	10	0.006658406	148.08148	1
TACACGC	10	0.006658406	148.08148	1
AGTATGG	10	0.006658406	148.08148	1
CGGGTGT	45	0.0	148.08148	1
GTCCCGC	10	0.006658406	148.08148	1
CCCCGCA	35	9.094947E-12	148.08148	1
AGTGTGT	10	0.006658406	148.08148	1
AGTGTGG	30	5.620677E-10	148.08148	1
TGCCAGA	15	1.13103895E-4	148.08148	1
AGGTTGT	10	0.006658406	148.08148	1
AGGTTGG	20	1.9185245E-6	148.08148	1
CGTGTGT	105	0.0	148.08148	1
CGTGTGG	115	0.0	148.08148	1
TCCCGCG	30	5.620677E-10	148.08148	1
CGTTGGG	10	0.006658406	148.08148	1
>>END_MODULE