FastQCFastQC Report
Wed 17 Jun 2015
000000000-AG1W8 l01n01 mis20.3410000000d2b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG1W8 l01n01 mis20.3410000000d2b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744609
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG21682029.118638104025063No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA14424519.371911969906353No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG13687418.381996457201026No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC9920013.322428281151586No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG57190.7680541062490516No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG50950.684251734803098No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG38600.5183928746496483No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG38040.5108721490070628No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG36800.49421911365562327No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG36340.488041374734928No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA35380.4751487022047813No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG34860.46816517125095186No Hit
TTCCAGATCTCGATGCCGACAGGCAGCGCTTTGTTTTTCAGTAAAGAAAC32900.44184263150190234No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCCTCCGCGGCTATATTGA30510.4097452488487246No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC26520.35616007864530247No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC25080.3368210698500824No Hit
TTCCAGATCTCGATGCCGACAGGGAAAGCAATAGAATCCAAAGTACCTGT21590.2899508332561116No Hit
TTCCAGATCTCGATGCCGACAGGGGAAAGCAATAGAATCCAAAGTACCTG21220.28498178238511757No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC17620.23663426039706748No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA17380.23341109226453077No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG17140.23018792413199413No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC15690.21071461666458502No Hit
AGTTTAGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA14800.19876203483976151No Hit
AGTTTGTAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA13600.18264619417707817No Hit
TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG10210.13711894430499766No Hit
TTCCAGATCTCGATGCCGACGACGGGAAAGCAATAGAATCCAAAGTACCT9810.13174699741743653No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA9300.12489776513579612No Hit
TTCCAGATCTCGATGCCGACAGGCAATGAATCCAAAGTACCTGTCTCTTA9080.12194319434763749No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC8000.10743893775122246No Hit
TCCCGCAGATCATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG7940.10663314571808828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTGT650.0148.081481
TTCCGCA359.094947E-12148.081481
TGCCGAG400.0148.081481
AGGGTGT100.006658406148.081481
TTTCAGA100.006658406148.081481
GGTTTGG100.006658406148.081481
TACACGC100.006658406148.081481
AGTATGG100.006658406148.081481
CGGGTGT450.0148.081481
GTCCCGC100.006658406148.081481
CCCCGCA359.094947E-12148.081481
AGTGTGT100.006658406148.081481
AGTGTGG305.620677E-10148.081481
TGCCAGA151.13103895E-4148.081481
AGGTTGT100.006658406148.081481
AGGTTGG201.9185245E-6148.081481
CGTGTGT1050.0148.081481
CGTGTGG1150.0148.081481
TCCCGCG305.620677E-10148.081481
CGTTGGG100.006658406148.081481
Produced by FastQC (version 0.11.2)