##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AG1W8 l01n01 mis19.3410000000d299.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	732897
Sequences flagged as poor quality	0
Sequence length	151
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42878057898995	34.0	34.0	34.0	32.0	34.0
2	33.477510482373376	34.0	34.0	34.0	34.0	34.0
3	33.616464523664305	34.0	34.0	34.0	34.0	34.0
4	33.61287056707832	34.0	34.0	34.0	34.0	34.0
5	33.72478260928889	34.0	34.0	34.0	34.0	34.0
6	36.42036329798048	38.0	38.0	38.0	34.0	38.0
7	36.878771505409354	38.0	38.0	38.0	34.0	38.0
8	37.00004775568736	38.0	38.0	38.0	35.0	38.0
9	37.41657831864505	38.0	38.0	38.0	37.0	38.0
10-14	37.28965543589345	38.0	38.0	38.0	36.4	38.0
15-19	37.255850958593086	38.0	38.0	38.0	36.2	38.0
20-24	37.5017665510979	38.0	38.0	38.0	37.2	38.0
25-29	37.258301234689185	38.0	38.0	38.0	36.4	38.0
30-34	37.010128844844495	38.0	38.0	38.0	35.8	38.0
35-39	37.09719947004832	38.0	38.0	38.0	36.0	38.0
40-44	36.998447257936654	38.0	38.0	38.0	36.0	38.0
45-49	37.07426254985353	38.0	38.0	38.0	36.0	38.0
50-54	37.05837409622361	38.0	38.0	38.0	35.8	38.0
55-59	36.68878027881134	38.0	37.8	38.0	34.8	38.0
60-64	36.600559150876585	38.0	37.2	38.0	34.4	38.0
65-69	36.66699931914034	38.0	37.4	38.0	34.8	38.0
70-74	36.8032359253756	38.0	38.0	38.0	35.6	38.0
75-79	36.40042407050377	38.0	37.4	38.0	33.8	38.0
80-84	36.65617678882571	38.0	37.2	38.0	34.4	38.0
85-89	36.32703313016699	38.0	37.0	38.0	33.4	38.0
90-94	36.55291944161321	38.0	37.0	38.0	34.6	38.0
95-99	36.447865934776644	38.0	37.2	38.0	34.0	38.0
100-104	36.00668170288595	38.0	36.8	38.0	32.4	38.0
105-109	36.1724212269937	38.0	37.0	38.0	33.0	38.0
110-114	36.09803014611876	38.0	37.0	38.0	33.0	38.0
115-119	35.84552727054415	38.0	36.8	38.0	31.0	38.0
120-124	36.005659185397136	38.0	36.8	38.0	32.4	38.0
125-129	35.92326452421008	38.0	37.0	38.0	31.6	38.0
130-134	35.452270100709924	38.0	36.4	38.0	29.6	38.0
135-139	35.21736314925563	38.0	36.0	38.0	28.8	38.0
140-144	35.71770630798053	38.0	37.0	38.0	31.0	38.0
145-149	35.36013327930119	38.0	36.2	38.0	29.0	38.0
150-151	33.452134474557816	36.0	33.0	37.5	24.5	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.48098923058837784
1101	2	0.2931011650423372
1101	3	0.05505924892975855
1101	4	0.0333067367564297
1101	5	0.09680987607627856
1101	6	1.0806725807552837
1101	7	-2.8664261532895807E-4
1101	8	0.3705505859629028
1101	9	0.10493029723413372
1101	10-14	0.23393034983367045
1101	15-19	0.2694119348339683
1101	20-24	0.16451541275439752
1101	25-29	0.25615684337079614
1101	30-34	0.23335967260115353
1101	35-39	0.23466419425979979
1101	40-44	0.19718818055446974
1101	45-49	0.313678852892032
1101	50-54	0.25444223377496655
1101	55-59	0.5057031537248591
1101	60-64	0.5415965387806878
1101	65-69	0.3509619875354133
1101	70-74	0.22449710823013191
1101	75-79	0.2871914641324764
1101	80-84	0.3827755525526655
1101	85-89	0.4374905791948862
1101	90-94	0.36048436241373594
1101	95-99	0.4398478326481339
1101	100-104	0.6942418294632944
1101	105-109	0.5063526461116794
1101	110-114	0.43135573739903066
1101	115-119	0.7302175016852672
1101	120-124	0.5037776312112499
1101	125-129	0.3707022161174933
1101	130-134	0.6637241280420056
1101	135-139	0.6697656216221546
1101	140-144	0.4751932919293651
1101	145-149	0.7859566037662589
1101	150-151	0.5044494401429702
1102	1	0.644152393751547
1102	2	0.31161968356085623
1102	3	0.11511930898981859
1102	4	0.14441784786753686
1102	5	0.1503634296298344
1102	6	1.0661580662407673
1102	7	0.4416552993266123
1102	8	0.25793797335029467
1102	9	0.19502038732422022
1102	10-14	0.2125089284122481
1102	15-19	0.269211734633771
1102	20-24	0.13588678412575916
1102	25-29	0.22132200853595663
1102	30-34	0.2009272401687312
1102	35-39	0.2701997297953298
1102	40-44	0.2988898822561694
1102	45-49	0.2862514254646058
1102	50-54	0.22120900054172665
1102	55-59	0.4431405911622974
1102	60-64	0.42798292516707903
1102	65-69	0.3760871126605352
1102	70-74	0.32409670782973166
1102	75-79	0.2285328054738187
1102	80-84	0.4300227997999144
1102	85-89	0.36882191052622204
1102	90-94	0.4085324104617811
1102	95-99	0.4407487335490359
1102	100-104	0.7517993870208457
1102	105-109	0.40224854200758386
1102	110-114	0.4372616433049288
1102	115-119	0.7899772614450313
1102	120-124	0.5796535070871229
1102	125-129	0.5399714853867579
1102	130-134	0.6214818857997599
1102	135-139	0.7499458018023333
1102	140-144	0.3939120106480871
1102	145-149	0.6674380852477384
1102	150-151	1.1711161068096416
1103	1	0.8763084899974132
1103	2	0.2815846610071304
1103	3	0.1435082964810519
1103	4	0.18264909657260375
1103	5	0.12486511335844597
1103	6	1.1556807560128348
1103	7	0.525816767527985
1103	8	0.3208854594898014
1103	9	0.11209406948518819
1103	10-14	0.20465683691926273
1103	15-19	0.2757326196085188
1103	20-24	0.04787368374358891
1103	25-29	0.2158571421733413
1103	30-34	0.3100498790170434
1103	35-39	0.14293106462142902
1103	40-44	0.30501091600812913
1103	45-49	0.23410196927524396
1103	50-54	0.20348179702898506
1103	55-59	0.3584046831844958
1103	60-64	0.4310063475920032
1103	65-69	0.30281508751819075
1103	70-74	0.28564229331546187
1103	75-79	0.24167906894427205
1103	80-84	0.3663631301552073
1103	85-89	0.4001917342152481
1103	90-94	0.31720751948601844
1103	95-99	0.4185916138358081
1103	100-104	0.5395648582277204
1103	105-109	0.433757707032953
1103	110-114	0.44843271462563195
1103	115-119	0.7801574665878235
1103	120-124	0.6943841978776675
1103	125-129	0.41352588766934417
1103	130-134	0.5694121602138296
1103	135-139	0.6246889239768834
1103	140-144	0.6522628802209027
1103	145-149	0.7492326978154225
1103	150-151	1.0218294136326733
2116	1	0.6645888137781313
2116	2	0.2723288558479027
2116	3	-0.12443838394901974
2116	4	-0.3375836208142573
2116	5	0.018828718689384516
2116	6	0.9140300160594421
2116	7	-0.14706046561865804
2116	8	0.04726418480765204
2116	9	0.03315667935395794
2116	10-14	0.10384370581260072
2116	15-19	0.23493032207367293
2116	20-24	0.0695700575631335
2116	25-29	0.22678341256293066
2116	30-34	0.13056856181824372
2116	35-39	0.06472468507848106
2116	40-44	0.05594909310637064
2116	45-49	0.23687674340549592
2116	50-54	0.13625437378382088
2116	55-59	0.3332454501196125
2116	60-64	0.3387959923326136
2116	65-69	0.33573866995553914
2116	70-74	0.25376961844526136
2116	75-79	0.21879335290649493
2116	80-84	0.3671079382170177
2116	85-89	0.4050017378945725
2116	90-94	0.36123254912504166
2116	95-99	0.33393895245647087
2116	100-104	0.514620712444632
2116	105-109	0.32593924090319604
2116	110-114	0.3341799755716295
2116	115-119	0.7341868398759175
2116	120-124	0.5465437579937742
2116	125-129	0.516733595857886
2116	130-134	0.5907142329009645
2116	135-139	0.7772468733657121
2116	140-144	0.6462172872361549
2116	145-149	0.7164781088779293
2116	150-151	1.485095301167938
1117	1	0.680719961319113
1117	2	0.2748739378151157
1117	3	0.07389358326409479
1117	4	0.059154084103774096
1117	5	0.11698855475496117
1117	6	1.0986570652397631
1117	7	0.390832977004294
1117	8	0.2756822175945359
1117	9	0.15041077771461175
1117	10-14	0.18510272220603952
1117	15-19	0.25027950240153984
1117	20-24	0.11564874608772868
1117	25-29	0.2101424289563738
1117	30-34	0.28802593886742756
1117	35-39	0.192132062127655
1117	40-44	0.2121859783522737
1117	45-49	0.31508575979894005
1117	50-54	0.2702314229641516
1117	55-59	0.47331266323437404
1117	60-64	0.48896320544735516
1117	65-69	0.33014276831619327
1117	70-74	0.3562679791010055
1117	75-79	0.25199827093928207
1117	80-84	0.33993170870882494
1117	85-89	0.38432141002571996
1117	90-94	0.3775743524037267
1117	95-99	-0.04714055574024911
1117	100-104	0.5200379255593859
1117	105-109	0.4371736671326971
1117	110-114	0.5885185001617828
1117	115-119	0.9399360202037883
1117	120-124	0.7161585120921288
1117	125-129	0.5599967106119834
1117	130-134	0.6620962001140782
1117	135-139	0.543682938939483
1117	140-144	0.5373967954328691
1117	145-149	-0.43029074358108943
1117	150-151	0.26674898969252325
2117	1	-30.288280038680888
2117	2	-10.455139659218261
2117	3	-1.9326088889237916
2117	4	-0.6415956068727411
2117	5	-0.05207634017705942
2117	6	1.1634265337193739
2117	7	-0.1754754284345239
2117	8	0.19810310758217753
2117	9	0.2671165873561492
2117	10-14	0.4102582228240905
2117	15-19	0.42536021933602797
2117	20-24	0.26707000690355187
2117	25-29	0.36053105689209275
2117	30-34	0.44536141476359603
2117	35-39	0.2975876863551008
2117	40-44	0.13046187452037827
2117	45-49	0.37274892419943484
2117	50-54	0.3396759223461032
2117	55-59	0.5097738498845601
2117	60-64	0.5158033785005145
2117	65-69	0.44435092653622377
2117	70-74	0.4148982634026055
2117	75-79	0.3922677394188838
2117	80-84	0.5997475307112907
2117	85-89	0.4757379736845522
2117	90-94	0.4601158851602136
2117	95-99	0.17420357281351784
2117	100-104	0.8726617821724858
2117	105-109	0.6813909724479004
2117	110-114	0.6974559538082659
2117	115-119	1.0592428187204703
2117	120-124	0.8307648161712393
2117	125-129	0.6680397266812079
2117	130-134	0.9869704893600613
2117	135-139	0.7233004914734664
2117	140-144	0.8673247311559891
2117	145-149	0.9408342255165607
2117	150-151	1.4449136992104528
1116	1	-0.10475322828840916
1116	2	-0.04827752708261812
1116	3	-0.4912034316612761
1116	4	-0.6950783647630061
1116	5	-0.11146556354244552
1116	6	1.069303002221524
1116	7	-0.5102966526253354
1116	8	0.18294506999917814
1116	9	0.10061254664772434
1116	10-14	0.227253523753852
1116	15-19	0.1939170369510137
1116	20-24	0.09431087986328635
1116	25-29	0.06689444664571198
1116	30-34	0.19022848170877182
1116	35-39	0.12066362652787888
1116	40-44	0.01687652561594888
1116	45-49	0.27779969014719796
1116	50-54	0.13368117420793624
1116	55-59	0.3805526300668731
1116	60-64	0.32645004900734165
1116	65-69	0.31549069534770524
1116	70-74	0.3124492394658418
1116	75-79	0.28808340084530215
1116	80-84	0.41002070815603275
1116	85-89	0.3733518135635876
1116	90-94	0.4420719201206964
1116	95-99	-0.059154154413555204
1116	100-104	0.7334003910099156
1116	105-109	0.47015973678444567
1116	110-114	0.44757344764659024
1116	115-119	0.926292681342531
1116	120-124	0.6428121881562561
1116	125-129	0.3665786896059018
1116	130-134	0.4554203018277292
1116	135-139	0.7300348460704882
1116	140-144	0.6656509137302749
1116	145-149	0.6496650883702628
1116	150-151	0.6488027486753865
2118	1	0.8907353765354245
2118	2	0.37790775183798786
2118	3	0.15866260365196183
2118	4	0.07533334815150994
2118	5	0.1009485249190547
2118	6	1.1598860557917305
2118	7	0.6489202470192055
2118	8	0.19412652390980156
2118	9	0.08317308502441279
2118	10-14	0.23867467645765572
2118	15-19	0.17676070578293945
2118	20-24	0.12330871625182027
2118	25-29	0.21941522855856732
2118	30-34	0.2931117668137233
2118	35-39	0.09885230081488317
2118	40-44	0.1770844368306328
2118	45-49	0.22332071753141491
2118	50-54	0.05442207438136393
2118	55-59	0.2580271833736063
2118	60-64	0.16261427456720412
2118	65-69	0.24869950625751613
2118	70-74	0.23654152460074584
2118	75-79	0.28499777367424173
2118	80-84	0.3119799434179171
2118	85-89	0.20924632300432933
2118	90-94	0.3169089620506682
2118	95-99	0.38030081671126226
2118	100-104	0.6329038131774851
2118	105-109	0.2760061882664715
2118	110-114	0.47741039474160374
2118	115-119	0.7787708287567483
2118	120-124	0.5981841212418075
2118	125-129	0.3791698582997043
2118	130-134	0.4026286217948396
2118	135-139	0.5839998956774224
2118	140-144	0.48337108683018926
2118	145-149	0.4846559993328796
2118	150-151	1.2367438927258405
1119	1	1.1691785248550204
1119	2	0.354271727870362
1119	3	0.11094054459006486
1119	4	-0.06464978329954363
1119	5	0.1373670077936282
1119	6	1.2353228110961183
1119	7	0.37900148529158173
1119	8	0.1767678529536525
1119	9	0.05281409263174197
1119	10-14	0.1938165343607281
1119	15-19	0.17465906041259416
1119	20-24	0.016412281999336642
1119	25-29	0.20837226321052782
1119	30-34	0.34028892229284224
1119	35-39	0.26231106765252576
1119	40-44	0.1290191275235344
1119	45-49	0.1444531631138588
1119	50-54	0.1366700969973067
1119	55-59	0.39267122677028254
1119	60-64	0.3651692827954278
1119	65-69	0.2641184589239387
1119	70-74	0.3559204652888539
1119	75-79	0.48294854718238156
1119	80-84	0.2803681727972176
1119	85-89	0.3887633989759962
1119	90-94	0.3441760098622879
1119	95-99	0.3780892785138903
1119	100-104	0.7198406879903203
1119	105-109	0.39831289365204725
1119	110-114	0.32163231231648126
1119	115-119	0.7624376776623549
1119	120-124	0.6037745341915794
1119	125-129	0.3824342796727649
1119	130-134	0.5883928851969529
1119	135-139	0.5084089057903043
1119	140-144	0.5011277346593914
1119	145-149	0.6160678199548215
1119	150-151	1.2015128018472225
2119	1	0.6313671930682574
2119	2	0.310116955943478
2119	3	0.11256849752181353
2119	4	0.13224644079167547
2119	5	0.11576293006118021
2119	6	1.2501154189879315
2119	7	0.4897396404045864
2119	8	0.3422116149487735
2119	9	0.1660085239174549
2119	10-14	0.29072212445984036
2119	15-19	0.26456401979497457
2119	20-24	0.1553695692136472
2119	25-29	0.23444208598724714
2119	30-34	0.19423642392376905
2119	35-39	0.21914156727220302
2119	40-44	0.0017473698270364935
2119	45-49	0.27051226641334836
2119	50-54	0.24172059493867692
2119	55-59	0.39919110517459444
2119	60-64	0.3013548272014006
2119	65-69	0.2802973983994832
2119	70-74	0.3153091843925253
2119	75-79	0.10551223468254989
2119	80-84	0.29023009185433324
2119	85-89	0.39306418317613634
2119	90-94	0.41882320100734916
2119	95-99	0.38957801358850475
2119	100-104	0.7905339079209739
2119	105-109	0.4902840052022768
2119	110-114	0.5208700925184715
2119	115-119	0.875046652246894
2119	120-124	0.6032768364429373
2119	125-129	0.6363873034586263
2119	130-134	0.6219304480317618
2119	135-139	0.5074161089541818
2119	140-144	0.4583146298277754
2119	145-149	0.5476734406423276
2119	150-151	0.5787417628429381
1118	1	0.46876563673213667
1118	2	0.292307368330178
1118	3	0.1323479078510701
1118	4	0.19746652336519332
1118	5	0.15498320975981983
1118	6	1.2303660059589134
1118	7	0.30300644639204677
1118	8	0.4545631699754864
1118	9	0.05771398471169675
1118	10-14	0.15479640539359707
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2104	150-151	-0.27038751337496336
2111	1	1.101323666928991
2111	2	0.373388091186186
2111	3	0.1294862235111367
2111	4	0.17633087257520685
2111	5	0.05881331028763981
2111	6	0.9547607708496457
2111	7	0.4857766208540042
2111	8	-0.22221304745950476
2111	9	-0.16909256762764358
2111	10-14	-0.1662654713091456
2111	15-19	-0.09420938076881669
2111	20-24	-0.06169834603785773
2111	25-29	0.030059464468472186
2111	30-34	0.04275512054524455
2111	35-39	-0.12670108249405843
2111	40-44	-0.09178705304248069
2111	45-49	-0.11430873325304702
2111	50-54	-0.3498322929390909
2111	55-59	-0.17619238051241126
2111	60-64	-0.23877745332772804
2111	65-69	-0.26156654717531325
2111	70-74	-0.12207926742498643
2111	75-79	-0.26158587728511407
2111	80-84	-0.1931321101280261
2111	85-89	-0.24140581591354504
2111	90-94	-0.6335194715798096
2111	95-99	-0.38863319598728907
2111	100-104	-0.3403877563757618
2111	105-109	-0.350598540793186
2111	110-114	-0.43104876695093
2111	115-119	-2.0058702587462918
2111	120-124	-0.9010851832243887
2111	125-129	-0.6479273243854422
2111	130-134	-0.5102151225827782
2111	135-139	-0.4082850487084926
2111	140-144	-0.821560538586688
2111	145-149	-1.1572733992681705
2111	150-151	-2.7038496979039763
2110	1	1.3487172803539664
2110	2	0.38624927293575695
2110	3	0.16043915967160416
2110	4	0.12703478115471256
2110	5	0.08889338049222317
2110	6	1.1179720786445912
2110	7	0.3949361941624687
2110	8	-0.023018854791523324
2110	9	-0.06391898099852256
2110	10-14	-0.03482328315589456
2110	15-19	-0.08157277641883809
2110	20-24	-0.1457973665128094
2110	25-29	-0.07101896514549821
2110	30-34	0.12541682358591544
2110	35-39	-0.3370389834487426
2110	40-44	-0.13573788223754235
2110	45-49	-0.14354292652814138
2110	50-54	-0.2682720622905421
2110	55-59	-0.5094522161221988
2110	60-64	-0.15570462297086607
2110	65-69	-0.16230548905645747
2110	70-74	-0.27294301285610345
2110	75-79	-0.3811223724923565
2110	80-84	-0.2694329025512374
2110	85-89	-0.27494936745417675
2110	90-94	-0.3623942803040521
2110	95-99	-0.6816772849627739
2110	100-104	-0.13223580098216559
2110	105-109	-0.2732161451997399
2110	110-114	-0.7288305615004091
2110	115-119	-6.678073631270745
2110	120-124	-2.9162530160580005
2110	125-129	-0.488583450245919
2110	130-134	-0.6177694835320295
2110	135-139	-0.8960269806315608
2110	140-144	-0.635297842636831
2110	145-149	-0.7740448990770687
2110	150-151	-1.3666444419428672
2109	1	1.0832079946177586
2109	2	0.4113474029555917
2109	3	-0.005507561376902004
2109	4	0.07264159711105833
2109	5	0.10743444445319028
2109	6	1.0984557124822203
2109	7	0.5626212184194799
2109	8	-0.019670019658320825
2109	9	-0.08212304122399416
2109	10-14	0.02066276382934973
2109	15-19	-0.08456056003342383
2109	20-24	-0.2695810145782431
2109	25-29	-0.1176748447168805
2109	30-34	-0.07498384261020163
2109	35-39	-0.2466875008275906
2109	40-44	-0.026137850992164147
2109	45-49	-0.17077740357255067
2109	50-54	-0.09008345736884138
2109	55-59	-0.3918632993046529
2109	60-64	-0.1693642659366077
2109	65-69	-0.23516670098661763
2109	70-74	-0.32315439342761465
2109	75-79	-0.16999756672981192
2109	80-84	-0.245251433851017
2109	85-89	-0.534904396425901
2109	90-94	-0.45556102740896876
2109	95-99	-0.22190163794628148
2109	100-104	-0.29686290690679584
2109	105-109	-0.3413047928048627
2109	110-114	-0.9158710940109458
2109	115-119	-4.745436716528793
2109	120-124	-1.3128440893646953
2109	125-129	-0.7793402300748014
2109	130-134	-1.0287199289181785
2109	135-139	-1.101782825888833
2109	140-144	-0.539201227459948
2109	145-149	-0.6589974033105435
2109	150-151	-2.04381578658219
2108	1	0.7643515402664818
2108	2	0.21076202008976708
2108	3	0.04880072180009165
2108	4	0.007998306729305682
2108	5	-0.0433257223170358
2108	6	0.7805230664496889
2108	7	0.020119425885113174
2108	8	-0.1750712609293572
2108	9	-0.2827077945781866
2108	10-14	-0.2322332729542751
2108	15-19	-0.3956278781187308
2108	20-24	-0.5784972914198363
2108	25-29	-0.37707184811561234
2108	30-34	-5.588274847581456
2108	35-39	-1.0459252881445664
2108	40-44	-0.7327817167475459
2108	45-49	-0.28631079192520303
2108	50-54	-0.297505903109645
2108	55-59	-0.5029361570923072
2108	60-64	-0.786897109132191
2108	65-69	-0.7015300689493813
2108	70-74	-0.5991873748009979
2108	75-79	-0.8424203618495838
2108	80-84	-0.6161802090165267
2108	85-89	-0.8317390860420346
2108	90-94	-0.9679164521939754
2108	95-99	-0.9129083718322022
2108	100-104	-0.702221058106673
2108	105-109	-0.8636124187717087
2108	110-114	-1.2351011005641652
2108	115-119	-1.014966339142859
2108	120-124	-1.3031817782889874
2108	125-129	-0.9184693510940036
2108	130-134	-1.1089708900250699
2108	135-139	-1.7829110114537485
2108	140-144	-1.7389680563517658
2108	145-149	-1.6796000599755274
2108	150-151	-2.3979066364417783
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	3.0
17	2.0
18	7.0
19	37.0
20	102.0
21	325.0
22	832.0
23	1243.0
24	1617.0
25	2184.0
26	2723.0
27	3728.0
28	5017.0
29	6639.0
30	8902.0
31	11831.0
32	16782.0
33	22848.0
34	34054.0
35	56350.0
36	116531.0
37	441139.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.1377112584185948	14.877699287166344	83.97261401641094	1.0119754380041215
2	52.16749420450623	0.07490820674665062	34.32719740973152	13.430400179015606
3	0.15609287526078017	0.05416859394976374	15.392340260636898	84.39739827015256
4	0.5404579361083481	0.30932040927988513	15.267220359750416	83.88300129486134
5	50.229568411386595	34.27343815024485	15.282365734884984	0.21462770348357274
6	49.66113928696665	36.935340163761076	0.4018299979396832	13.001690551332587
7	15.39684293973096	47.14195855625006	0.8455485559362366	36.61564994808275
8	49.82801130308898	15.699886887243364	33.88784508600799	0.5842567236596684
9	36.61455838951449	28.73377841633954	0.5899874061430188	34.06167578800295
10-14	12.348611059944304	49.34131262646729	22.065965613176203	16.2441107004122
15-19	34.05875586883286	18.079566432936687	23.526143509933863	24.33553418829658
20-24	26.594050732913356	26.85782586093271	12.459649855300267	34.088473550853664
25-29	25.147681052044145	40.25474248086702	13.779153141573783	20.818423325515045
30-34	15.656852190689824	17.239721270519595	40.520809881879714	26.58261665691086
35-39	31.94148700294857	17.639286284430145	20.614001694644678	29.805225017976607
40-44	8.685204060052094	33.55161775802057	32.52858177888571	25.234596403041625
45-49	10.328872952133793	25.325659676598484	30.532585069934793	33.812882301332934
50-54	20.451004711439673	33.766927685609296	17.08755800610454	28.694509596846483
55-59	20.679140452205424	29.446102249019983	42.6095890691325	7.265168229642091
60-64	21.038918156302998	26.249527559807177	24.834267298133298	27.877286985756527
65-69	16.49836197992351	40.40338546889945	23.790846462736237	19.307406088440803
70-74	16.275847765784277	12.525934749357686	38.28990976903985	32.90830771581818
75-79	26.71199363621355	30.99616999387363	20.32069990735397	21.97113646255886
80-84	3.630523798023461	30.346747223689004	40.14989828038592	25.872830697901616
85-89	26.55352662106681	33.047918056698286	25.35152961466618	15.047025707568732
90-94	13.817412269391196	50.49599056893397	23.057946751044145	12.62865041063069
95-99	10.774720049338447	44.02866978579528	30.365331008313586	14.831279156552693
100-104	16.351792953170772	40.260391296457755	17.347076055707692	26.040739694663777
105-109	16.764893293327713	30.93796263322131	35.235838050913024	17.061306022537956
110-114	40.64980481568352	15.006283284008532	15.438103853611079	28.905808046696873
115-119	13.213180023932422	47.875949826510414	17.869304963726144	21.041565185831026
120-124	4.159493080200901	19.667811438715127	44.32483691432766	31.847858566756308
125-129	22.753183598786734	34.234032885930766	6.089750674378528	36.92303284090397
130-134	20.42538037404983	45.46229552038008	26.809469816358916	7.302854289211172
135-139	38.19911938512506	9.722648612287948	34.554596348463704	17.523635654123293
140-144	12.731693539474168	46.66429252678071	17.82777116020396	22.776242773541167
145-149	28.00696414366548	28.865966158955487	28.584835249700845	14.542234447678187
150-151	42.3154959018798	17.227864215571902	21.35074915028988	19.10589073225842
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.5
26	0.5
27	0.0
28	1.0
29	3.0
30	7.5
31	20.0
32	44.0
33	132.5
34	571.5
35	5099.0
36	121264.5
37	128740.5
38	13119.5
39	1346.5
40	920.0
41	4168.5
42	44213.5
43	49632.0
44	11570.5
45	3665.0
46	4103.0
47	28782.5
48	32312.0
49	26571.5
50	124346.5
51	116140.5
52	13077.0
53	1628.0
54	517.0
55	173.5
56	72.0
57	177.0
58	267.0
59	144.0
60	29.5
61	17.5
62	8.0
63	5.5
64	3.0
65	0.5
66	0.5
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.207677204300195
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	732897.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	1.389572316298608
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.15747131291064	1.0026802455053159
2	9.379343284706025	0.2606655154697749
3	3.723656877713973	0.15522871537998745
4	2.207322921521895	0.12268939299512761
5	1.4876993878761058	0.10336329421835107
6	0.9937737741971056	0.08285523151727292
7	0.8655390938996514	0.08419104144899958
8	0.6294163320679093	0.06996956083742228
9	0.46221373756244066	0.057805147253670974
>10	4.6024159254746735	1.43130976617629
>50	1.366956287148366	1.3748715614089146
>100	1.7898276565539755	4.735866100008682
>500	0.10817639682469084	1.0370639379318412
>1k	0.14751326839730566	3.955027604641368
>5k	0.03933687157261485	3.0486044790250943
>10k+	0.03933687157261485	82.4778084061819
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	232388	31.708139070019385	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	207678	28.336587542314952	No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	89264	12.179610504613882	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	74224	10.127480396290338	No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	6077	0.8291751774123786	No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC	5620	0.7668198942006859	No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	5424	0.7400767092783843	No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG	5188	0.7078757315147968	No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	4145	0.5655637831782638	No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC	3822	0.5214921059848792	No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	2121	0.2893994654091912	No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	2113	0.288307906840934	No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG	2090	0.2851696759571945	No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	1959	0.2672954044019828	No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCCTCCGCGGCTATATTGA	1891	0.2580171565717966	No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC	1846	0.25187713962534986	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG	1809	0.24682868124716023	No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	1747	0.2383691023431669	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA	1144	0.15609287526078017	No Hit
TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	1128	0.15390975812426577	No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA	1099	0.1499528583143334	No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	1018	0.13890082781072918	No Hit
TTCCATATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	1010	0.137809269242472	No Hit
AGTTTAGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	967	0.13194214193808954	No Hit
AGTTTGTAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	822	0.11215764288842771	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10-11	0.0	0.0	0.0
12-13	0.0	0.0	6.822241051607525E-5
14-15	0.0	0.0	1.364448210321505E-4
16-17	0.0	0.0	1.364448210321505E-4
18-19	0.0	0.0	0.0012280033892893545
20-21	0.0	0.0	0.015281819955600856
22-23	0.0	0.0	0.03561209828939128
24-25	0.0	0.0	0.04400345478286853
26-27	0.0	0.0	0.04720990807712407
28-29	0.0	0.0	0.06508417963233579
30-31	0.0	0.0	0.07361198094684519
32-33	0.0	0.0	0.08807513197625313
34-35	0.0	0.0	0.10847363272055964
36-37	0.0	0.0	0.11283986699358846
38-39	0.0	0.0	0.12252744928687115
40-41	0.0	0.0	0.13317014532737886
42-43	0.0	0.0	0.15697976659748913
44-45	0.0	0.0	0.22138172212466417
46-47	0.0	0.0	0.24928468802573894
48-49	0.0	0.0	0.27500453679029935
50-51	0.0	0.0	0.3553023139677199
52-53	0.0	0.0	0.3563938725359771
54-55	0.0	0.0	0.3563938725359771
56-57	0.0	0.0	0.3563938725359771
58-59	0.0	0.0	0.35728076387268604
60-61	0.0	0.0	0.3611012188615863
62-63	0.0	0.0	0.37986238175350695
64-65	0.0	0.0	0.37986238175350695
66-67	0.0	0.0	0.3804081610376356
68-69	0.0	0.0	0.3810903851427963
70-71	0.0	0.0	0.38238661094260173
72-73	0.0	0.0	0.3824548333531178
74-75	0.0	0.0	0.3828641678162143
76-77	0.0	0.0	0.383546391921375
78-79	0.0	0.0	0.3841603936160197
80-81	0.0	0.0	0.38456972807911616
82-83	0.0	0.0	0.38463795048963223
84-85	0.0	0.0	0.38518372977376086
86-87	0.0	0.0	0.38593417628943766
88-89	0.0	0.0	0.3883901830680164
90-91	0.0	0.0	0.39023218815195043
92-93	0.0	0.0	0.39023218815195043
94-95	0.0	0.0	0.39023218815195043
96-97	0.0	0.0	0.3903686329729826
98-99	0.0	0.0	0.3905050777940147
100-101	0.0	0.0	0.3905050777940147
102-103	0.0	0.0	0.39180130359382015
104-105	0.0	0.0	0.3933021966251738
106-107	0.0	0.0	0.3937797534987863
108-109	0.0	0.0	0.3937797534987863
110-111	0.0	0.0	0.3937797534987863
112-113	0.0	0.0	0.3939161983198185
114-115	0.0	0.0	0.3939161983198185
116-117	0.0	0.0	0.3939161983198185
118-119	0.0	0.0	0.3945984224249792
120-121	0.0	0.0	0.39541709135117215
122-123	0.0	0.0	0.39875998946645985
124-125	0.0	0.0	0.39978332562420094
126-127	0.0	0.0	0.39978332562420094
128-129	0.0	0.0	0.39978332562420094
130-131	0.0	0.0	0.4012159962450385
132-133	0.0	0.0	0.4026486668658761
134-135	0.0	0.0	0.4026486668658761
136-137	0.0	0.0	0.4026486668658761
138-139	0.0	0.0	0.40271688927639215
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTGG	340	0.0	148.25516	1
AGCTTGG	30	5.5661076E-10	148.25516	1
TTCCGAG	30	5.5661076E-10	148.25516	1
CGGGTGT	15	1.1257619E-4	148.25516	1
AGTCTGG	15	1.1257619E-4	148.25516	1
CGTTTGT	45	0.0	148.25514	1
TGCACGG	10	0.006635089	148.25514	1
TTCCGCA	25	3.2498065E-8	148.25514	1
TGCCGCG	20	1.9073432E-6	148.25514	1
CCCCGCA	25	3.2498065E-8	148.25514	1
TTCCACT	10	0.006635089	148.25514	1
CGTGTGT	50	0.0	148.25514	1
CGTGTGG	45	0.0	148.25514	1
TCCCGGA	205	0.0	148.25514	1
TCCCGCG	35	9.094947E-12	148.25514	1
TCCCGAA	80	0.0	148.25514	1
CGTTGGG	20	1.9073432E-6	148.25514	1
TCCTGCA	10	0.006635089	148.25514	1
AGTTTTG	35	9.094947E-12	148.25514	1
AGTTTGT	175	0.0	148.25514	1
>>END_MODULE