FastQCFastQC Report
Wed 17 Jun 2015
000000000-AG1W8 l01n01 mis19.3410000000d299.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG1W8 l01n01 mis19.3410000000d299.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732897
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG23238831.708139070019385No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC20767828.336587542314952No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA8926412.179610504613882No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG7422410.127480396290338No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG60770.8291751774123786No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC56200.7668198942006859No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC54240.7400767092783843No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG51880.7078757315147968No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG41450.5655637831782638No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC38220.5214921059848792No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG21210.2893994654091912No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA21130.288307906840934No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG20900.2851696759571945No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG19590.2672954044019828No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCCTCCGCGGCTATATTGA18910.2580171565717966No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC18460.25187713962534986No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG18090.24682868124716023No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC17470.2383691023431669No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA11440.15609287526078017No Hit
TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG11280.15390975812426577No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA10990.1499528583143334No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG10180.13890082781072918No Hit
TTCCATATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC10100.137809269242472No Hit
AGTTTAGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA9670.13194214193808954No Hit
AGTTTGTAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA8220.11215764288842771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTGG3400.0148.255161
AGCTTGG305.5661076E-10148.255161
TTCCGAG305.5661076E-10148.255161
CGGGTGT151.1257619E-4148.255161
AGTCTGG151.1257619E-4148.255161
CGTTTGT450.0148.255141
TGCACGG100.006635089148.255141
TTCCGCA253.2498065E-8148.255141
TGCCGCG201.9073432E-6148.255141
CCCCGCA253.2498065E-8148.255141
TTCCACT100.006635089148.255141
CGTGTGT500.0148.255141
CGTGTGG450.0148.255141
TCCCGGA2050.0148.255141
TCCCGCG359.094947E-12148.255141
TCCCGAA800.0148.255141
CGTTGGG201.9073432E-6148.255141
TCCTGCA100.006635089148.255141
AGTTTTG359.094947E-12148.255141
AGTTTGT1750.0148.255141
Produced by FastQC (version 0.11.2)