FastQCFastQC Report
Wed 17 Jun 2015
000000000-AG1W8 l01n01 mis18.3410000000d28c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG1W8 l01n01 mis18.3410000000d28c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674031
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC17702326.263332101935966No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG13814120.4947546922916No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG11911417.671887494788816No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA11072416.4271376242339No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC50230.7452179499162501No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC48510.7196998357642305No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG38380.5694100122991376No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG34080.5056147269190883No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG34010.5045761990175526No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG32310.4793548071231145No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG29500.4376653299328963No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG28160.4177849386749274No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG28100.41689477190218255No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA25570.379359406318107No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCCTCCGCGGCTATATTGA23820.3533962087797149No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC19530.2897492845284564No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG17880.26526969827797237No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC16370.24286716783055973No Hit
AGTTTAGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA14540.21571708126184108No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA13700.20325474644341282No Hit
AGTTTGTAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA11510.17076365923822495No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC10960.16260379715473028No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA9560.14183323912401655No Hit
TTCCATATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC8990.13337665478294025No Hit
TTCCAGATCTCGATGCCCACAGGCAGAGAATTTTCCGGCACATATTCGCC7820.1160184027144152No Hit
TTCCAGATCTCGATTCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC7770.11527659707046116No Hit
TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG7430.11023231869157354No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA7380.10949051304761948No Hit
TTCCACATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC7220.10711673498696647No Hit
TCCCGCAGATCATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG7150.10607820708543078No Hit
TCCCGCAGATGATGAAGCTGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG6930.10281426225203293No Hit
AGTTTGGAAACTCCGATCTTTTTCCTTTTACTGTTTGTGATCAATTTGCC6920.10266590112324211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTGT700.0148.244191
TCCCACA359.094947E-12148.244191
CGTGTGG650.0148.244191
TCCCGTA750.0148.244191
AGTTTGC359.094947E-12148.244191
AGCTTGG253.250898E-8148.244171
TGCGACG305.5661076E-10148.244171
CTCCAGA100.0066364375148.244171
TTTCAGA151.1260533E-4148.244171
ACCCGCA201.9079307E-6148.244171
CGGGTGT151.1260533E-4148.244171
TCTCGCA151.1260533E-4148.244171
AGTGTGT151.1260533E-4148.244171
AGTGTGG305.5661076E-10148.244171
TGCCAGA100.0066364375148.244171
CGTATGG100.0066364375148.244171
CGTGTGT950.0148.244171
TCCCGCG400.0148.244171
TCCCGAA2500.0148.244171
AGTCTGG100.0066364375148.244171
Produced by FastQC (version 0.11.2)