##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AG1W8 l01n01 mis18.3410000000d28c.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	674031
Sequences flagged as poor quality	0
Sequence length	151
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07198185246673	34.0	34.0	34.0	31.0	34.0
2	33.40823641642595	34.0	34.0	34.0	33.0	34.0
3	33.4324623051462	34.0	34.0	34.0	33.0	34.0
4	33.45463635945528	34.0	34.0	34.0	34.0	34.0
5	33.67104035274342	34.0	34.0	34.0	34.0	34.0
6	36.35261434563099	38.0	38.0	38.0	34.0	38.0
7	36.70650311335829	38.0	38.0	38.0	34.0	38.0
8	36.80961558147919	38.0	37.0	38.0	34.0	38.0
9	37.35045865842966	38.0	38.0	38.0	36.0	38.0
10-14	37.22389593356982	38.0	38.0	38.0	36.2	38.0
15-19	37.182157793929356	38.0	38.0	38.0	36.2	38.0
20-24	37.465415982350954	38.0	38.0	38.0	36.6	38.0
25-29	37.1809145276701	38.0	38.0	38.0	36.4	38.0
30-34	36.80680028069926	38.0	37.8	38.0	35.2	38.0
35-39	36.99716926966268	38.0	37.8	38.0	35.8	38.0
40-44	36.76388919797457	38.0	37.8	38.0	35.0	38.0
45-49	36.950711762515375	38.0	37.8	38.0	35.8	38.0
50-54	36.93755954844807	38.0	37.8	38.0	35.4	38.0
55-59	36.594882431223496	38.0	37.4	38.0	34.2	38.0
60-64	36.460750618295	38.0	37.0	38.0	34.0	38.0
65-69	36.69720324436116	38.0	37.2	38.0	34.8	38.0
70-74	36.73192390260982	38.0	37.2	38.0	34.8	38.0
75-79	36.35056488499787	38.0	37.2	38.0	33.8	38.0
80-84	36.58393664386357	38.0	37.2	38.0	34.2	38.0
85-89	36.23687812578353	38.0	37.0	38.0	33.0	38.0
90-94	36.5517048919115	38.0	37.0	38.0	34.6	38.0
95-99	36.33302800613028	38.0	37.0	38.0	33.8	38.0
100-104	35.85638583388598	38.0	36.4	38.0	31.4	38.0
105-109	36.073972265370585	38.0	37.0	38.0	32.4	38.0
110-114	36.16058044808028	38.0	37.0	38.0	33.0	38.0
115-119	35.739719389761	38.0	36.8	38.0	30.2	38.0
120-124	35.90080604601271	38.0	36.6	38.0	31.2	38.0
125-129	35.92034698700802	38.0	37.0	38.0	31.8	38.0
130-134	35.33806813039756	38.0	36.0	38.0	28.8	38.0
135-139	35.243076950466666	38.0	36.2	38.0	28.6	38.0
140-144	35.551721805080184	38.0	36.4	38.0	30.4	38.0
145-149	35.154568261697165	37.8	35.4	38.0	28.0	38.0
150-151	33.47619471507987	36.0	32.5	37.5	24.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.5374875036217759
1101	2	0.35410926593564085
1101	3	0.1950622998646523
1101	4	0.14904307288434637
1101	5	0.19771227518390333
1101	6	1.046754473684011
1101	7	-0.017320492005040933
1101	8	0.24721621919108117
1101	9	0.16279871128006818
1101	10-14	0.26368412197822977
1101	15-19	0.30283245657321345
1101	20-24	0.15884498789992563
1101	25-29	0.2418506026193583
1101	30-34	0.25168685660232626
1101	35-39	0.042730461002534526
1101	40-44	0.16797351171971542
1101	45-49	0.24062091364593385
1101	50-54	0.18969093519125835
1101	55-59	0.36036411556891323
1101	60-64	0.422738438706844
1101	65-69	0.21403032355044616
1101	70-74	0.1717776348837745
1101	75-79	0.20871238553328908
1101	80-84	0.320881515931994
1101	85-89	0.3918235748775345
1101	90-94	0.2623253583142926
1101	95-99	0.4538869144799733
1101	100-104	0.6508849639381964
1101	105-109	0.45914474489386947
1101	110-114	0.33815685343642343
1101	115-119	0.684680572237383
1101	120-124	0.5243475507882422
1101	125-129	0.4482309020065429
1101	130-134	0.5669503105743701
1101	135-139	0.6071617426393985
1101	140-144	0.5504474356160713
1101	145-149	0.6462395628527204
1101	150-151	0.5400668660976464
1102	1	0.4604613324439768
1102	2	0.32029389929818564
1102	3	0.15392847138363663
1102	4	0.21754916413908632
1102	5	0.20806516919628137
1102	6	1.1933518793831155
1102	7	0.44517932140830396
1102	8	0.30649000259275994
1102	9	0.2329456989184422
1102	10-14	0.3214643591458639
1102	15-19	0.3358530706672269
1102	20-24	0.17080188744806435
1102	25-29	0.304894715970228
1102	30-34	0.26987884254542394
1102	35-39	0.3023132319103752
1102	40-44	0.3468358022362139
1102	45-49	0.3341104253433329
1102	50-54	0.2564665684663723
1102	55-59	0.473510158545686
1102	60-64	0.502416379472507
1102	65-69	0.4367255518365596
1102	70-74	0.23941401906890292
1102	75-79	0.25297969704344325
1102	80-84	0.4652607794371306
1102	85-89	0.3739144543084265
1102	90-94	0.45545918253047546
1102	95-99	0.519618195573301
1102	100-104	0.844196312032139
1102	105-109	0.5381094981410115
1102	110-114	0.4562519209381364
1102	115-119	0.8600556114205844
1102	120-124	0.7204172894176466
1102	125-129	0.6297199061882139
1102	130-134	0.765621067220593
1102	135-139	0.8837815813858896
1102	140-144	0.6351423184503062
1102	145-149	0.8445237119429194
1102	150-151	1.304158956742512
1103	1	0.9057417659402844
1103	2	0.35149867855347594
1103	3	0.23562062256755212
1103	4	0.2366184342749662
1103	5	0.19272982201515276
1103	6	1.1640763455577385
1103	7	0.4511777850708043
1103	8	0.23715550662564056
1103	9	0.18204517677130383
1103	10-14	0.15792060985953782
1103	15-19	0.29010727223885624
1103	20-24	0.0914787063989948
1103	25-29	0.21794922480935952
1103	30-34	0.40038500346639694
1103	35-39	0.19221326616654721
1103	40-44	0.3488289925240622
1103	45-49	0.2910591345084086
1103	50-54	0.252483924927823
1103	55-59	0.416745498460358
1103	60-64	0.5516911866517802
1103	65-69	0.3231884461718266
1103	70-74	0.3517326430087735
1103	75-79	0.31515797447693217
1103	80-84	0.3513050218047553
1103	85-89	0.43973738103136384
1103	90-94	0.3797823116762373
1103	95-99	0.44446212236889693
1103	100-104	0.6750645503305464
1103	105-109	0.4680689097652788
1103	110-114	0.3960847923321609
1103	115-119	0.9165446650159197
1103	120-124	0.7212944550825782
1103	125-129	0.5473600710330828
1103	130-134	0.6441076709804676
1103	135-139	0.6419995336971382
1103	140-144	0.7309241377645606
1103	145-149	0.8571062122283948
1103	150-151	0.826058513488384
2116	1	0.6553934561876105
2116	2	0.3121639771818394
2116	3	-0.05416229135403938
2116	4	-0.3967004986551501
2116	5	0.0619408599242135
2116	6	1.0676350452013281
2116	7	-0.14745327659891672
2116	8	0.2488441339072054
2116	9	0.15151812201376913
2116	10-14	0.2555893645846723
2116	15-19	0.31539186020731336
2116	20-24	0.1393834477317597
2116	25-29	0.29657704638835725
2116	30-34	0.3359509393125961
2116	35-39	0.17414328020694114
2116	40-44	0.18188061796957555
2116	45-49	0.2900541663692948
2116	50-54	0.27032224440076646
2116	55-59	0.4829632325569051
2116	60-64	0.45164798544213625
2116	65-69	0.39020486263149223
2116	70-74	0.2722580777209487
2116	75-79	0.3502087358982493
2116	80-84	0.4986443673064258
2116	85-89	0.48662689393772496
2116	90-94	0.46212867737447993
2116	95-99	0.5930516028743469
2116	100-104	0.86816315709153
2116	105-109	0.5443282250261063
2116	110-114	0.4569898576739817
2116	115-119	0.939662712321244
2116	120-124	0.7057771205972188
2116	125-129	0.642171065740925
2116	130-134	0.7567375446169251
2116	135-139	0.9223381231776457
2116	140-144	0.8884989298587485
2116	145-149	1.0349396715652475
2116	150-151	1.588968924979902
1117	1	0.4548237063434044
1117	2	0.28417501608507223
1117	3	0.16457268594498942
1117	4	0.19905976129321346
1117	5	0.16275987532526415
1117	6	1.139541481594101
1117	7	0.28924885106065545
1117	8	0.2975955805376742
1117	9	0.13514449074875046
1117	10-14	0.23036947675417707
1117	15-19	0.25259118362937727
1117	20-24	0.13872378424025555
1117	25-29	0.27175197739521906
1117	30-34	0.35670363227085033
1117	35-39	0.22034465116750113
1117	40-44	0.25889610804345864
1117	45-49	0.34928099802602475
1117	50-54	0.30235776474260945
1117	55-59	0.5529169403820688
1117	60-64	0.5920031888606445
1117	65-69	0.41573766775924526
1117	70-74	0.42997818810998467
1117	75-79	0.36236354761373235
1117	80-84	0.482613031064993
1117	85-89	0.534063464755846
1117	90-94	0.39766148250223665
1117	95-99	-0.022486988775256123
1117	100-104	0.5749173634478026
1117	105-109	0.5783485223643012
1117	110-114	0.6778182205334176
1117	115-119	1.0600081231642946
1117	120-124	0.8206551585211912
1117	125-129	0.6726424447135955
1117	130-134	0.8052348294028278
1117	135-139	0.7013423963014773
1117	140-144	0.6625045383337778
1117	145-149	-0.3752406008463893
1117	150-151	0.3541718983619049
2117	1	-29.953649097003876
2117	2	-10.379691926142808
2117	3	-2.124131650765282
2117	4	-0.8048617769690622
2117	5	-0.1082683265252058
2117	6	1.126452089386774
2117	7	-0.13067039523813406
2117	8	0.19433896509760729
2117	9	0.19712534975639073
2117	10-14	0.4090223991483981
2117	15-19	0.3510056742447105
2117	20-24	0.2650594020285979
2117	25-29	0.3770745980048673
2117	30-34	0.45641727761906026
2117	35-39	0.28921759079369025
2117	40-44	0.3304017084308981
2117	45-49	0.4630851505908282
2117	50-54	0.37567103166343685
2117	55-59	0.5166171943563995
2117	60-64	0.5426997880823805
2117	65-69	0.5081351910871561
2117	70-74	0.4974310395490775
2117	75-79	0.43726795527913964
2117	80-84	0.6545338034623143
2117	85-89	0.45192599762890495
2117	90-94	0.5156983059647331
2117	95-99	0.1369710209895274
2117	100-104	0.9526321012798391
2117	105-109	0.7083162265151799
2117	110-114	0.6657089665196665
2117	115-119	1.1077572686054182
2117	120-124	0.9303303979947728
2117	125-129	0.6186148961008797
2117	130-134	0.9377443886362826
2117	135-139	0.7829931731475739
2117	140-144	1.024087400979269
2117	145-149	1.1161956493436733
2117	150-151	1.4771161000321626
1116	1	-0.16742155209369614
1116	2	-0.2044878255955851
1116	3	-0.7557803318687562
1116	4	-1.019748020632477
1116	5	-0.2078355447133191
1116	6	1.0470910268550426
1116	7	-0.6700325860650906
1116	8	0.11454995630496256
1116	9	-0.04428389098151797
1116	10-14	0.23468160563449203
1116	15-19	0.2395185005436673
1116	20-24	0.06465418285724667
1116	25-29	0.009806120155722908
1116	30-34	0.18973422380744154
1116	35-39	0.18296860827420858
1116	40-44	0.0865875870102144
1116	45-49	0.2288049626490789
1116	50-54	0.17798500398796335
1116	55-59	0.31169428109812003
1116	60-64	0.31312588071541825
1116	65-69	0.3028109270983066
1116	70-74	0.2774568291542536
1116	75-79	0.21838238859287173
1116	80-84	0.3083468771802913
1116	85-89	0.3547718805602216
1116	90-94	0.41617186758979585
1116	95-99	-0.2567190685487617
1116	100-104	0.7150353701122967
1116	105-109	0.42253500295959867
1116	110-114	0.4732135804412181
1116	115-119	0.9571872377384949
1116	120-124	0.6407503655965741
1116	125-129	0.46058296534597787
1116	130-134	0.4043155800354512
1116	135-139	0.6284197896319696
1116	140-144	0.5619433176788533
1116	145-149	0.5406728859874761
1116	150-151	0.48069239293760546
2118	1	0.69516980063392
2118	2	0.43404927343470234
2118	3	0.13050850777479184
2118	4	0.12902687053008322
2118	5	0.15897414299787016
2118	6	1.2431948821366063
2118	7	0.615233103029162
2118	8	0.3646466133845081
2118	9	0.10546071429611459
2118	10-14	0.29837626356273006
2118	15-19	0.26837951866490783
2118	20-24	0.12948955149005315
2118	25-29	0.2820455782474056
2118	30-34	0.3595168632529564
2118	35-39	0.0846358250218131
2118	40-44	0.05071822337528431
2118	45-49	0.28645250221561724
2118	50-54	0.09340455857957153
2118	55-59	0.3729006871835878
2118	60-64	0.2796102348418188
2118	65-69	0.378250598935729
2118	70-74	0.33108060522416594
2118	75-79	0.31156044193499355
2118	80-84	0.3076355439412666
2118	85-89	0.3346857536015406
2118	90-94	0.3588883244398744
2118	95-99	0.45627101427771066
2118	100-104	0.8318779055139345
2118	105-109	0.4519819358654402
2118	110-114	0.5260678718583947
2118	115-119	0.9494873618996209
2118	120-124	0.5422497290743493
2118	125-129	0.4048339908540157
2118	130-134	0.5776064785533777
2118	135-139	0.690412339964368
2118	140-144	0.5336575270553681
2118	145-149	0.5286795498251493
2118	150-151	1.1393577394100163
1119	1	1.2807351980585864
1119	2	0.3447610888301753
1119	3	0.14110774800955994
1119	4	-0.17132553188005772
1119	5	0.15860159884607583
1119	6	1.2383939652970142
1119	7	0.3481423668250798
1119	8	-9.391131468490244E-4
1119	9	-0.012428743453845925
1119	10-14	0.20933833619478293
1119	15-19	0.17616862682292833
1119	20-24	0.010996216220625854
1119	25-29	0.15736007827161558
1119	30-34	0.26381473619136386
1119	35-39	0.16910410646284646
1119	40-44	0.18625915404865623
1119	45-49	0.13683995750172784
1119	50-54	0.06057114639678929
1119	55-59	0.19246378314103652
1119	60-64	0.3080156490668884
1119	65-69	0.19978015342850597
1119	70-74	0.29279999172857174
1119	75-79	0.3914312225133614
1119	80-84	0.20613490611943774
1119	85-89	0.40195173857173927
1119	90-94	0.25674858447131754
1119	95-99	0.2683648029705452
1119	100-104	0.6567879624885222
1119	105-109	0.3452518773607025
1119	110-114	0.32589337255242157
1119	115-119	0.7671540738461715
1119	120-124	0.6037858413434876
1119	125-129	0.2374381306845379
1119	130-134	0.435540435500009
1119	135-139	0.29318178260022165
1119	140-144	0.5364085454624217
1119	145-149	0.5874841296512443
1119	150-151	0.924789326969595
2119	1	0.5371355264821673
2119	2	0.27805419935924647
2119	3	0.17016966387435417
2119	4	0.22513630234873716
2119	5	0.07521352302796913
2119	6	1.2224404859148095
2119	7	0.602045059591461
2119	8	0.34412524490628016
2119	9	0.0853349124857985
2119	10-14	0.1989084126838918
2119	15-19	0.19135580539683872
2119	20-24	0.08954859624216738
2119	25-29	0.16399007452187675
2119	30-34	0.23441894599024948
2119	35-39	0.17786898130382411
2119	40-44	-0.08717515606602433
2119	45-49	0.24918713668962766
2119	50-54	0.10437941894951308
2119	55-59	0.4062004346677668
2119	60-64	0.19555306426302366
2119	65-69	0.2679029232008858
2119	70-74	0.280335621332938
2119	75-79	0.04183854105886553
2119	80-84	0.2578994623732598
2119	85-89	0.44570199505476893
2119	90-94	0.24269140355735885
2119	95-99	0.331356533801042
2119	100-104	0.745851526109945
2119	105-109	0.3834588424843446
2119	110-114	0.44958551272199543
2119	115-119	0.9369624109308177
2119	120-124	0.5339546048274428
2119	125-129	0.43738506414994305
2119	130-134	0.395228351648619
2119	135-139	0.199192178503516
2119	140-144	0.16143659021749812
2119	145-149	0.375348621404477
2119	150-151	0.3003185434801239
1118	1	0.2662053728505924
1118	2	0.32121648304285344
1118	3	0.09750312201349942
1118	4	0.15183314136784531
1118	5	0.24751944177938867
1118	6	1.3168122911870839
1118	7	0.45297026478206703
1118	8	0.5630554401397916
1118	9	0.13258814457315538
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2111	3	0.13942787478163154
2111	4	0.19719606022474068
2111	5	0.12876285916738794
2111	6	0.9958396569614365
2111	7	0.491199510842641
2111	8	-0.11739132464432345
2111	9	-0.09586472243393018
2111	10-14	-0.1324987324844571
2111	15-19	-0.07553146836533386
2111	20-24	-0.058171147549067825
2111	25-29	-0.03617578866003868
2111	30-34	-0.14498429097023546
2111	35-39	-0.21545389267913606
2111	40-44	-0.20447813009258198
2111	45-49	-0.2390248854000916
2111	50-54	-0.4377979567686552
2111	55-59	-0.31580960957147397
2111	60-64	-0.3714874743300811
2111	65-69	-0.3692286769981479
2111	70-74	-0.3536282639985089
2111	75-79	-0.4048199502750123
2111	80-84	-0.3425756495390715
2111	85-89	-0.44710670306427147
2111	90-94	-0.7212979775735704
2111	95-99	-0.5741019963248775
2111	100-104	-0.6195452449055239
2111	105-109	-0.5128973337439291
2111	110-114	-0.6669785997423006
2111	115-119	-2.3504505675459484
2111	120-124	-1.1403182029461973
2111	125-129	-0.8594280968669494
2111	130-134	-0.6317301254554692
2111	135-139	-0.58378808377784
2111	140-144	-1.1549736229665442
2111	145-149	-1.4683007673942328
2111	150-151	-2.5089823024221047
2110	1	1.4632639721859562
2110	2	0.43698499458309925
2110	3	0.2063747193534624
2110	4	0.17057305218345675
2110	5	0.09992197395720126
2110	6	1.181663632088103
2110	7	0.2508100574474099
2110	8	-0.2256940972791739
2110	9	-0.13570270917009708
2110	10-14	-0.12318317338228724
2110	15-19	-0.20066825781425024
2110	20-24	-0.1657839591910033
2110	25-29	-0.24952390105555367
2110	30-34	-0.012495969856097133
2110	35-39	-0.27926625965994134
2110	40-44	-0.2539475461533769
2110	45-49	-0.24783912351330883
2110	50-54	-0.2454452732458563
2110	55-59	-0.4403704363032688
2110	60-64	-0.29977645181643453
2110	65-69	-0.32036467320906326
2110	70-74	-0.21302061926056126
2110	75-79	-0.33953401986757115
2110	80-84	-0.26027764786776686
2110	85-89	-0.26473802924761003
2110	90-94	-0.34598077567482477
2110	95-99	-0.6552898040735613
2110	100-104	-0.007538240797160256
2110	105-109	-0.24106220239315235
2110	110-114	-0.7224399144613471
2110	115-119	-6.553406854974959
2110	120-124	-2.994338321606193
2110	125-129	-0.43582237890343833
2110	130-134	-0.659247205688196
2110	135-139	-0.8813397333091402
2110	140-144	-0.5645661707363985
2110	145-149	-0.6746658746934102
2110	150-151	-1.3927517300523746
2109	1	1.110941445095314
2109	2	0.405106135271609
2109	3	0.02700289061361616
2109	4	0.1273467172274465
2109	5	0.11327034040225925
2109	6	1.0616729357017078
2109	7	0.7229282051552346
2109	8	3.748463173280925E-4
2109	9	-0.05067456983142904
2109	10-14	0.0022117356858046833
2109	15-19	-0.12454948804662536
2109	20-24	-0.32539188417823794
2109	25-29	-0.09606338650570478
2109	30-34	-0.11250558722792903
2109	35-39	-0.3704862812546921
2109	40-44	-0.16519673362186893
2109	45-49	-0.3681942569048928
2109	50-54	-0.14888153974118268
2109	55-59	-0.615373659145952
2109	60-64	-0.3744267648584838
2109	65-69	-0.3491898464411207
2109	70-74	-0.4272784753232912
2109	75-79	-0.3770519330752151
2109	80-84	-0.3624286651606283
2109	85-89	-0.5488981496216354
2109	90-94	-0.594313321202165
2109	95-99	-0.40645047079616603
2109	100-104	-0.33442929916505904
2109	105-109	-0.35353859379154073
2109	110-114	-1.033470767126623
2109	115-119	-5.216584802955204
2109	120-124	-1.4548347962596395
2109	125-129	-0.8311447211495562
2109	130-134	-0.940431465665533
2109	135-139	-0.9685103639017925
2109	140-144	-0.7721414539543545
2109	145-149	-0.677375722677553
2109	150-151	-1.6458555492403235
2108	1	0.6269960642864447
2108	2	0.0763369353149912
2108	3	-0.012501344202355824
2108	4	0.1092706749893182
2108	5	0.008491992388179881
2108	6	0.7976624988732581
2108	7	0.0984458311567451
2108	8	-0.3286266965663671
2108	9	-0.3010899768194477
2108	10-14	-0.2163426875773098
2108	15-19	-0.3353356620987924
2108	20-24	-0.5168897163039361
2108	25-29	-0.31204683830621605
2108	30-34	-5.96563794195027
2108	35-39	-0.9959528076448123
2108	40-44	-0.8184042138766898
2108	45-49	-0.22761570322575864
2108	50-54	-0.27034693885250505
2108	55-59	-0.4235501511081523
2108	60-64	-0.6332936755050156
2108	65-69	-0.585385391314972
2108	70-74	-0.5313074679207759
2108	75-79	-0.5380445242252989
2108	80-84	-0.37132474731265575
2108	85-89	-0.8004218363299529
2108	90-94	-0.8783071496673784
2108	95-99	-0.8109497698092412
2108	100-104	-0.6739919561548646
2108	105-109	-0.9343093865044381
2108	110-114	-1.0919051320308881
2108	115-119	-0.9953338559697684
2108	120-124	-1.1149332431639394
2108	125-129	-0.7365420433874164
2108	130-134	-1.1204210003384674
2108	135-139	-1.9457504149143219
2108	140-144	-1.6442086885352154
2108	145-149	-1.39383177661594
2108	150-151	-2.149852282981662
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	3.0
16	2.0
17	1.0
18	9.0
19	16.0
20	66.0
21	243.0
22	476.0
23	893.0
24	1271.0
25	1845.0
26	2390.0
27	3366.0
28	4769.0
29	6332.0
30	8887.0
31	12198.0
32	16884.0
33	23981.0
34	35045.0
35	57640.0
36	117371.0
37	380342.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.13319801143561058	20.45757612994543	78.22773825832222	1.1814876002967374
2	45.358447905215044	0.06246003522093198	31.910846830487028	22.668245229077
3	0.14895457330597553	0.0627567574785136	21.171578161835285	78.61671050738023
4	0.6545693002250638	0.2990960356422776	20.935090522542733	78.11124414158994
5	46.88730933740437	31.896010717607943	20.986275111975562	0.23040483301213147
6	52.99919439907067	24.736547725549716	0.4449350252436461	21.819322850135972
7	20.70854307887916	54.1402398406008	1.0382311792781045	24.11298590124193
8	46.50765320882868	21.5411457336532	31.563681789116526	0.38751926840160167
9	24.130789236696828	43.66253184200727	0.624303629951738	31.582375291344167
10-14	15.570470794369992	39.31632224630618	25.945661252969078	19.16754570635475
15-19	31.10385130654228	24.197492400201178	22.694060065486603	22.00459622776994
20-24	26.697258731423336	25.203974297918048	16.94355304132896	31.155213929329662
25-29	30.338693620916544	37.39798317881522	13.502257314574553	18.761065885693682
30-34	17.922469441316498	15.406383385927352	39.091703497316885	27.579443675439258
35-39	35.3665632589599	14.400791654983228	18.599975372052622	31.63266971400425
40-44	12.694341951631305	32.684876511614455	25.6145785579595	29.006202978794743
45-49	10.90394952160954	18.883434144720347	38.69412534438327	31.51849098928684
50-54	18.42645219581889	33.27033919804875	17.27264769721274	31.030560908919618
55-59	21.082294434528976	29.4502775094914	42.64121383141132	6.826214224568307
60-64	24.459438809194236	28.47139078172962	19.172352606927575	27.896817802148565
65-69	19.21439815082689	38.413040349776196	22.297490768228762	20.07507073116815
70-74	15.902265622797765	13.69607629322687	35.72776326311401	34.673894820861356
75-79	25.060776136409157	28.526937188348906	25.84964193041566	20.56264474482628
80-84	4.6886567531760415	29.24684472969344	31.879690993440956	34.184807523689564
85-89	26.157520944882357	34.73098418322006	29.081273709962897	10.030221161934689
90-94	12.940057653134648	48.93306094230087	22.891795777939	15.23508562662548
95-99	9.378856462091505	43.59835081769236	33.95351252390469	13.069280196311444
100-104	19.384034265486306	38.012702679847074	16.197711974671787	26.405551079994837
105-109	14.342099992433582	35.608599604469234	32.801696064424334	17.24760433867285
110-114	37.177103130271455	11.701064194376816	26.20639110070605	24.915441574645676
115-119	13.756399928193213	44.47626296119911	17.214312101372194	24.55302500923548
120-124	4.519436049677241	20.411850493523296	46.78410933621747	28.284604120581992
125-129	21.941958159194456	35.19722386655806	9.12788877662897	33.7329291976185
130-134	18.982450362075333	54.618407758693586	19.64586198557633	6.753279893654743
135-139	31.418881327416692	13.186070076895573	37.560023203680544	17.835025392007193
140-144	13.940367727893822	37.85903022264554	26.13360513092128	22.066996918539356
145-149	23.56330791907197	34.26106514388804	30.541414267296314	11.634212669743677
150-151	37.565037809833676	33.41381924570235	16.40080352387353	12.620339420590449
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	0.5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	0.5
21	0.0
22	0.5
23	0.5
24	0.0
25	0.0
26	0.5
27	0.5
28	1.5
29	3.5
30	3.5
31	4.5
32	27.5
33	86.0
34	359.0
35	3318.0
36	71975.5
37	77194.5
38	8998.0
39	1040.5
40	1077.5
41	5701.0
42	55362.0
43	61922.5
44	14636.0
45	4668.0
46	8122.5
47	67446.0
48	69643.0
49	17256.5
50	95775.5
51	96729.5
52	10348.5
53	1161.0
54	373.5
55	291.0
56	161.0
57	61.5
58	71.5
59	62.0
60	18.0
61	38.0
62	37.5
63	10.0
64	6.0
65	10.5
66	7.5
67	5.5
68	8.0
69	3.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.5
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.2049430960890524
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	674031.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	1.5228603282215842
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.7653630620643	1.1081148467585797
2	9.065020092121589	0.27609518945647077
3	3.8813813944609934	0.1773240523296606
4	1.8972859048194144	0.11557205742973917
5	1.2324781480835347	0.0938446038558211
6	0.9279142752386245	0.08478503026508309
7	0.8103674363734887	0.08638534940950704
8	0.5857374725362191	0.07135970877425502
9	0.5564312903494004	0.07626304237088227
>10	4.724720370324158	1.6019475900457367
>50	1.6204612414898278	1.7773646546355961
>100	1.5814139826587477	4.928485312406651
>500	0.13666540590878065	1.48970986986478
>1k	0.1659508500320908	6.34669029778835
>5k	0.009761814707770046	0.7467131699761291
>10k+	0.039047258831080185	81.01934522463276
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	177023	26.263332101935966	No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	138141	20.4947546922916	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	119114	17.671887494788816	No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	110724	16.4271376242339	No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC	5023	0.7452179499162501	No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	4851	0.7196998357642305	No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	3838	0.5694100122991376	No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG	3408	0.5056147269190883	No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	3401	0.5045761990175526	No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	3231	0.4793548071231145	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG	2950	0.4376653299328963	No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG	2816	0.4177849386749274	No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	2810	0.41689477190218255	No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	2557	0.379359406318107	No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCCTCCGCGGCTATATTGA	2382	0.3533962087797149	No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC	1953	0.2897492845284564	No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	1788	0.26526969827797237	No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	1637	0.24286716783055973	No Hit
AGTTTAGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	1454	0.21571708126184108	No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA	1370	0.20325474644341282	No Hit
AGTTTGTAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	1151	0.17076365923822495	No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC	1096	0.16260379715473028	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA	956	0.14183323912401655	No Hit
TTCCATATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	899	0.13337665478294025	No Hit
TTCCAGATCTCGATGCCCACAGGCAGAGAATTTTCCGGCACATATTCGCC	782	0.1160184027144152	No Hit
TTCCAGATCTCGATTCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	777	0.11527659707046116	No Hit
TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	743	0.11023231869157354	No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA	738	0.10949051304761948	No Hit
TTCCACATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	722	0.10711673498696647	No Hit
TCCCGCAGATCATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	715	0.10607820708543078	No Hit
TCCCGCAGATGATGAAGCTGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	693	0.10281426225203293	No Hit
AGTTTGGAAACTCCGATCTTTTTCCTTTTACTGTTTGTGATCAATTTGCC	692	0.10266590112324211	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	1.4836112879081228E-4
9	0.0	0.0	1.4836112879081228E-4
10-11	0.0	0.0	1.4836112879081228E-4
12-13	0.0	0.0	4.450833863724369E-4
14-15	0.0	0.0	4.450833863724369E-4
16-17	0.0	0.0	4.450833863724369E-4
18-19	0.0	0.0	7.418056439540614E-4
20-21	0.0	0.0	0.0023737780606529965
22-23	0.0	0.0	0.009495112242611986
24-25	0.0	0.0	0.011720529174474172
26-27	0.0	0.0	0.012017251432055795
28-29	0.0	0.0	0.015726279651826103
30-31	0.0	0.0	0.016023001909407725
32-33	0.0	0.0	0.01884186335643316
34-35	0.0	0.0	0.02692754487553243
36-37	0.0	0.0	0.030265670273325707
38-39	0.0	0.0	0.03338125397793276
40-41	0.0	0.0	0.03508740695902711
42-43	0.0	0.0	0.04154111606142744
44-45	0.0	0.0	0.0530391035427154
46-47	0.0	0.0	0.07544163399012804
48-49	0.0	0.0	0.10288844281642832
50-51	0.0	0.0	0.14932547612795258
52-53	0.0	0.0	0.3248366914874835
54-55	0.0	0.0	0.3259493999534146
56-57	0.0	0.0	0.32624612221099625
58-59	0.0	0.0	0.32639448333978704
60-61	0.0	0.0	0.33915354041579693
62-63	0.0	0.0	0.35272858370015625
64-65	0.0	0.0	0.35295112539334245
66-67	0.0	0.0	0.35636343135553117
68-69	0.0	0.0	0.37231225270054347
70-71	0.0	0.0	0.37253479439372966
72-73	0.0	0.0	0.3726831555225205
74-75	0.0	0.0	0.3729798777801021
76-77	0.0	0.0	0.37342496116647456
78-79	0.0	0.0	0.37342496116647456
80-81	0.0	0.0	0.37364750285966075
82-83	0.0	0.0	0.373870044552847
84-85	0.0	0.0	0.373870044552847
86-87	0.0	0.0	0.3739442251172424
88-89	0.0	0.0	0.3740184056816378
90-91	0.0	0.0	0.3740184056816378
92-93	0.0	0.0	0.3740184056816378
94-95	0.0	0.0	0.3741667668104286
96-97	0.0	0.0	0.37446348906801025
98-99	0.0	0.0	0.37446348906801025
100-101	0.0	0.0	0.37446348906801025
102-103	0.0	0.0	0.3767630865642678
104-105	0.0	0.0	0.3787659618029438
106-107	0.0	0.0	0.3789143229317346
108-109	0.0	0.0	0.3790626840605254
110-111	0.0	0.0	0.3798786702688749
112-113	0.0	0.0	0.38039793421964274
114-115	0.0	0.0	0.38039793421964274
116-117	0.0	0.0	0.38047211478403814
118-119	0.0	0.0	0.38655492106446143
120-121	0.0	0.0	0.388854518560719
122-123	0.0	0.0	0.39523404709872395
124-125	0.0	0.0	0.6404749929899367
126-127	0.0	0.0	0.6413651597626815
128-129	0.0	0.0	0.6415135208914724
130-131	0.0	0.0	0.6419586042778448
132-133	0.0	0.0	0.6424036876642172
134-135	0.0	0.0	0.6424778682286126
136-137	0.0	0.0	0.6425520487930081
138-139	0.0	0.0	0.6547176613538547
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTGT	70	0.0	148.24419	1
TCCCACA	35	9.094947E-12	148.24419	1
CGTGTGG	65	0.0	148.24419	1
TCCCGTA	75	0.0	148.24419	1
AGTTTGC	35	9.094947E-12	148.24419	1
AGCTTGG	25	3.250898E-8	148.24417	1
TGCGACG	30	5.5661076E-10	148.24417	1
CTCCAGA	10	0.0066364375	148.24417	1
TTTCAGA	15	1.1260533E-4	148.24417	1
ACCCGCA	20	1.9079307E-6	148.24417	1
CGGGTGT	15	1.1260533E-4	148.24417	1
TCTCGCA	15	1.1260533E-4	148.24417	1
AGTGTGT	15	1.1260533E-4	148.24417	1
AGTGTGG	30	5.5661076E-10	148.24417	1
TGCCAGA	10	0.0066364375	148.24417	1
CGTATGG	10	0.0066364375	148.24417	1
CGTGTGT	95	0.0	148.24417	1
TCCCGCG	40	0.0	148.24417	1
TCCCGAA	250	0.0	148.24417	1
AGTCTGG	10	0.0066364375	148.24417	1
>>END_MODULE