##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AG1W8 l01n01 mis17.3410000000d27f.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	750347
Sequences flagged as poor quality	0
Sequence length	151
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.382742917610116	34.0	34.0	34.0	32.0	34.0
2	33.47430722052597	34.0	34.0	34.0	34.0	34.0
3	33.58679251066506	34.0	34.0	34.0	34.0	34.0
4	33.58040746481295	34.0	34.0	34.0	34.0	34.0
5	33.72565226488545	34.0	34.0	34.0	34.0	34.0
6	36.40319878669469	38.0	38.0	38.0	34.0	38.0
7	36.884704010277915	38.0	38.0	38.0	34.0	38.0
8	36.9192293698782	38.0	38.0	38.0	35.0	38.0
9	37.385628249329976	38.0	38.0	38.0	37.0	38.0
10-14	37.23233757181677	38.0	38.0	38.0	36.4	38.0
15-19	37.21955441948859	38.0	38.0	38.0	36.2	38.0
20-24	37.48974034679954	38.0	38.0	38.0	37.0	38.0
25-29	37.218095361212875	38.0	38.0	38.0	36.4	38.0
30-34	36.954885672895344	38.0	38.0	38.0	35.8	38.0
35-39	37.06955155414762	38.0	38.0	38.0	36.0	38.0
40-44	36.979759764482296	38.0	38.0	38.0	35.6	38.0
45-49	37.06966989939322	38.0	38.0	38.0	36.0	38.0
50-54	37.02677267984012	38.0	38.0	38.0	36.0	38.0
55-59	36.70575453756729	38.0	37.8	38.0	34.8	38.0
60-64	36.640960249058104	38.0	37.0	38.0	34.4	38.0
65-69	36.760373533845005	38.0	37.4	38.0	35.0	38.0
70-74	36.80715055834168	38.0	37.8	38.0	35.6	38.0
75-79	36.41014837135352	38.0	37.2	38.0	33.8	38.0
80-84	36.60296396200691	38.0	37.2	38.0	34.2	38.0
85-89	36.37938260564779	38.0	37.0	38.0	33.4	38.0
90-94	36.592525591493	38.0	37.0	38.0	34.6	38.0
95-99	36.41693882963483	38.0	37.0	38.0	34.0	38.0
100-104	36.038105303279686	38.0	36.8	38.0	32.4	38.0
105-109	36.151823089850424	38.0	37.0	38.0	32.8	38.0
110-114	36.197505154281956	38.0	37.0	38.0	33.4	38.0
115-119	35.79212684264747	38.0	36.8	38.0	30.4	38.0
120-124	35.94929359349741	38.0	36.8	38.0	31.8	38.0
125-129	35.81276449429397	38.0	37.0	38.0	31.2	38.0
130-134	35.31949937828764	38.0	36.4	38.0	28.8	38.0
135-139	35.16313945414588	38.0	36.2	38.0	28.4	38.0
140-144	35.56108946927221	38.0	37.0	38.0	30.2	38.0
145-149	35.15630021843227	37.8	35.6	38.0	27.8	38.0
150-151	33.26412379872246	36.0	32.5	37.5	24.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.5380186093256825
1101	2	0.36440546449895095
1101	3	0.12154557781442321
1101	4	0.11792091077384015
1101	5	0.17441907474393759
1101	6	1.1787509867261718
1101	7	0.14961047816283468
1101	8	0.417119778023455
1101	9	0.20126092967087317
1101	10-14	0.277318585955733
1101	15-19	0.34491075534910465
1101	20-24	0.18612540848680936
1101	25-29	0.2955098751690741
1101	30-34	0.2346854876754776
1101	35-39	0.13797313852049342
1101	40-44	0.195261952614473
1101	45-49	0.26480208179376064
1101	50-54	0.2027310665783233
1101	55-59	0.37425638963450325
1101	60-64	0.42769163464231497
1101	65-69	0.32785642928829617
1101	70-74	0.20166316408449347
1101	75-79	0.2722645130259451
1101	80-84	0.3618512184902869
1101	85-89	0.40209560969753966
1101	90-94	0.32950301321849196
1101	95-99	0.40035814555103144
1101	100-104	0.592013662090281
1101	105-109	0.41322908978732187
1101	110-114	0.3951259521619903
1101	115-119	0.7342551387351932
1101	120-124	0.5392787737347078
1101	125-129	0.4039762753749372
1101	130-134	0.5315104047468324
1101	135-139	0.5529701579767305
1101	140-144	0.4835620200871702
1101	145-149	0.663329880289588
1101	150-151	0.53892758918618
1102	1	0.6137015647523967
1102	2	0.2739756111070193
1102	3	0.10086603831886976
1102	4	0.10973375467501967
1102	5	0.19570032589796682
1102	6	0.9955352106635402
1102	7	0.4596853978422928
1102	8	0.250063664429895
1102	9	0.206189564406273
1102	10-14	0.20669707112874391
1102	15-19	0.22016833502339495
1102	20-24	0.1165060078442508
1102	25-29	0.27067827134322897
1102	30-34	0.08360079104709683
1102	35-39	0.1448944151555267
1102	40-44	0.1599806460669555
1102	45-49	0.1474804026327874
1102	50-54	0.09142793500034685
1102	55-59	0.23666323626842
1102	60-64	0.25315486260494424
1102	65-69	0.20839951618219743
1102	70-74	0.108335677840536
1102	75-79	0.05026471613535932
1102	80-84	0.32985105230984857
1102	85-89	0.16883741146273934
1102	90-94	0.27163700081035813
1102	95-99	0.2861975413928235
1102	100-104	0.5454331938307462
1102	105-109	0.21724728654496772
1102	110-114	0.16309351928041593
1102	115-119	0.46967192849847805
1102	120-124	0.3421520796174917
1102	125-129	0.34763589168277775
1102	130-134	0.446774862444677
1102	135-139	0.485648894266852
1102	140-144	0.34132670859118264
1102	145-149	0.487565911900802
1102	150-151	0.9077202883257343
1103	1	0.680713262233752
1103	2	0.35117109007375547
1103	3	0.09255940079201963
1103	4	0.10236339320771748
1103	5	0.14438835701594144
1103	6	1.200746351476198
1103	7	0.49804769075893773
1103	8	0.32023523621423067
1103	9	0.1805260034302023
1103	10-14	0.24129901490029226
1103	15-19	0.32721219374809607
1103	20-24	0.11745183492981681
1103	25-29	0.2743769980031914
1103	30-34	0.3646386753295232
1103	35-39	0.26808428335366585
1103	40-44	0.3738367052172009
1103	45-49	0.258108054276569
1103	50-54	0.23794728627445494
1103	55-59	0.40517111575009324
1103	60-64	0.5095725436663372
1103	65-69	0.36925348811181635
1103	70-74	0.34857649226754717
1103	75-79	0.406541358848699
1103	80-84	0.45478604908293363
1103	85-89	0.4096764966489346
1103	90-94	0.47430072686304925
1103	95-99	0.507107400600141
1103	100-104	0.6584414846264224
1103	105-109	0.5163486406095359
1103	110-114	0.5116636319618664
1103	115-119	0.911969932908022
1103	120-124	0.6603749091870057
1103	125-129	0.4665097981649211
1103	130-134	0.47068695810738603
1103	135-139	0.6073728177273168
1103	140-144	0.6008842435356527
1103	145-149	0.7812192780749712
1103	150-151	0.7907444416307214
2116	1	0.7335264964236856
2116	2	0.29590420409103757
2116	3	0.05434626149023103
2116	4	-0.31197749270946673
2116	5	0.014918272666172072
2116	6	1.07847598863588
2116	7	-0.05073374687727039
2116	8	0.10605396945268097
2116	9	0.11543192501117971
2116	10-14	0.21103335182541372
2116	15-19	0.27909454573944004
2116	20-24	0.10984867256136255
2116	25-29	0.2152383145549095
2116	30-34	0.2737159284363031
2116	35-39	0.10402357393414263
2116	40-44	0.026344948565885318
2116	45-49	0.2571472998825257
2116	50-54	0.20758573772643985
2116	55-59	0.3712493619055337
2116	60-64	0.45377014277767813
2116	65-69	0.38129858420388274
2116	70-74	0.299689670844856
2116	75-79	0.2796938537943987
2116	80-84	0.4008977008063752
2116	85-89	0.408793818390528
2116	90-94	0.336835627228119
2116	95-99	0.44133324294618603
2116	100-104	0.6647031052191466
2116	105-109	0.4100821758770081
2116	110-114	0.3726424711444949
2116	115-119	0.6741708250845946
2116	120-124	0.5025994711604156
2116	125-129	0.4206865152343866
2116	130-134	0.5317541790956142
2116	135-139	0.5625353172464571
2116	140-144	0.5920403639875573
2116	145-149	0.722892747908567
2116	150-151	1.4513932144248969
1117	1	0.5795941528240718
1117	2	0.334730637640682
1117	3	0.15961754737110567
1117	4	0.20220297440111068
1117	5	0.1911119750068906
1117	6	1.2074647380687082
1117	7	0.4024337532267168
1117	8	0.4267384032595558
1117	9	0.19872537543214008
1117	10-14	0.23500015330509427
1117	15-19	0.24247380856935763
1117	20-24	0.14116499035414876
1117	25-29	0.25926732859672086
1117	30-34	0.34374154115497646
1117	35-39	0.23136889238146097
1117	40-44	0.23035630097193405
1117	45-49	0.29762878265669457
1117	50-54	0.35720599343287773
1117	55-59	0.5121507268812735
1117	60-64	0.5239651522898114
1117	65-69	0.39383079134896093
1117	70-74	0.37306477883949896
1117	75-79	0.36452526262780793
1117	80-84	0.46009693161202136
1117	85-89	0.48455917586174735
1117	90-94	0.3581669052965637
1117	95-99	-0.03950871123205246
1117	100-104	0.508348334880182
1117	105-109	0.5751116589514353
1117	110-114	0.5606163506657467
1117	115-119	0.947412012554679
1117	120-124	0.7364934602277984
1117	125-129	0.5582165349949548
1117	130-134	0.5825956886252044
1117	135-139	0.5162971643839
1117	140-144	0.5473444725889394
1117	145-149	-0.46006138299695465
1117	150-151	0.08553558518632798
2117	1	-30.246116006058934
2117	2	-10.443816553004506
2117	3	-2.0115987105319633
2117	4	-0.5271735157577098
2117	5	-0.05608530111972243
2117	6	1.2343935477579535
2117	7	-0.14825053058889637
2117	8	0.1402600359690993
2117	9	0.23175954781918762
2117	10-14	0.3980555735190663
2117	15-19	0.40773894512340547
2117	20-24	0.24881138269224579
2117	25-29	0.39042696406819744
2117	30-34	0.5223628253079013
2117	35-39	0.27035280226991887
2117	40-44	0.21424283699954572
2117	45-49	0.38484871928800146
2117	50-54	0.2843305598993737
2117	55-59	0.5194222118362575
2117	60-64	0.4726496033203489
2117	65-69	0.4336423574320065
2117	70-74	0.5150510037897007
2117	75-79	0.44509615742943964
2117	80-84	0.5486579895635231
2117	85-89	0.4421952181728912
2117	90-94	0.4345151044207043
2117	95-99	0.11408684200427643
2117	100-104	0.860579990970983
2117	105-109	0.6414804716390066
2117	110-114	0.6125827692969494
2117	115-119	1.028785884935104
2117	120-124	0.8669255954758412
2117	125-129	0.6769858331823997
2117	130-134	1.0172779082014571
2117	135-139	0.8043831013208163
2117	140-144	0.8494348897324997
2117	145-149	0.9955258729197141
2117	150-151	1.4937122506325196
1116	1	-0.08579429024123897
1116	2	-0.03156277637545912
1116	3	-0.5751070825279143
1116	4	-0.8192413907316265
1116	5	-0.15349905270502262
1116	6	1.1017660413766137
1116	7	-0.4165204251161967
1116	8	0.13137271677371132
1116	9	0.054475509996713356
1116	10-14	0.21374259090520553
1116	15-19	0.2449767483373506
1116	20-24	0.03844006099247821
1116	25-29	0.06282169203852561
1116	30-34	0.16943543611845513
1116	35-39	0.16906176504142678
1116	40-44	0.005656363833274725
1116	45-49	0.20577212716393234
1116	50-54	0.13730675228671174
1116	55-59	0.245892809514892
1116	60-64	0.31051927951754266
1116	65-69	0.2508557590470062
1116	70-74	0.19227050580443006
1116	75-79	0.0763215350923403
1116	80-84	0.2842666649740906
1116	85-89	0.2916251518711732
1116	90-94	0.31995955064888904
1116	95-99	-0.2841716544077286
1116	100-104	0.5450127340638744
1116	105-109	0.3442872976652396
1116	110-114	0.3063976061112541
1116	115-119	0.8809696675040115
1116	120-124	0.6273224425324031
1116	125-129	0.35331660327759806
1116	130-134	0.524788142424697
1116	135-139	0.5922715603277382
1116	140-144	0.5438610508151598
1116	145-149	0.48992763240342185
1116	150-151	0.4583259187386659
2118	1	0.6572043064410664
2118	2	0.36402986353741085
2118	3	0.15042177973749915
2118	4	0.06367661990845619
2118	5	0.1321789906093187
2118	6	1.2502729218223223
2118	7	0.4797937714320639
2118	8	0.1761191770619135
2118	9	0.12142318928625428
2118	10-14	0.24892465566727395
2118	15-19	0.2352803466952551
2118	20-24	0.08354578108296806
2118	25-29	0.28987435834214637
2118	30-34	0.2585301391177879
2118	35-39	0.06454465294357448
2118	40-44	0.10572920021062515
2118	45-49	0.24354456977452799
2118	50-54	0.15931009302062904
2118	55-59	0.2936914857883508
2118	60-64	0.28741218752693243
2118	65-69	0.2993603355382959
2118	70-74	0.3652433924498766
2118	75-79	0.3505878303336303
2118	80-84	0.32418895887489896
2118	85-89	0.18455955200522567
2118	90-94	0.2821262103338782
2118	95-99	0.5024885542048736
2118	100-104	0.774772075551823
2118	105-109	0.35023479892194587
2118	110-114	0.47168094014275397
2118	115-119	0.9563675185428835
2118	120-124	0.6627613458500932
2118	125-129	0.46722897970186494
2118	130-134	0.5620332412852918
2118	135-139	0.5763370449959666
2118	140-144	0.6002672484525604
2118	145-149	0.4996129331742054
2118	150-151	1.3216837490419522
1119	1	1.114053485466492
1119	2	0.3375964684102897
1119	3	0.061074586940890185
1119	4	-0.10112243994323933
1119	5	0.08541157800338794
1119	6	1.2136352761655402
1119	7	0.2631127597795242
1119	8	0.18173027346021797
1119	9	0.0693508905574376
1119	10-14	0.16900046340033015
1119	15-19	0.15945606173762883
1119	20-24	0.030035121654997
1119	25-29	0.16232701988875675
1119	30-34	0.2109583038423608
1119	35-39	0.0790275713757751
1119	40-44	0.055336920019946945
1119	45-49	0.14827013916011822
1119	50-54	0.05794456071131293
1119	55-59	0.23083396460190642
1119	60-64	0.31214404663710127
1119	65-69	0.19821659877981546
1119	70-74	0.16748617581851732
1119	75-79	0.33202983245227813
1119	80-84	0.19999007116303602
1119	85-89	0.2952719460730222
1119	90-94	0.27729636393558366
1119	95-99	0.27333402560317666
1119	100-104	0.5177209632636846
1119	105-109	0.32579590856177276
1119	110-114	0.29486519596903094
1119	115-119	0.7742535090089859
1119	120-124	0.6396780566551783
1119	125-129	0.44648116455356046
1119	130-134	0.6142776798446192
1119	135-139	0.5036758294239334
1119	140-144	0.6311646928381478
1119	145-149	0.6686509363521722
1119	150-151	1.097194375366108
2119	1	0.7405703844736138
2119	2	0.3367920522701553
2119	3	0.16048788550673265
2119	4	0.1961260389789672
2119	5	0.13314688539482233
2119	6	1.2918673575348478
2119	7	0.498710576782166
2119	8	0.3465823026782857
2119	9	0.09576290205745863
2119	10-14	0.2065493551865032
2119	15-19	0.1897825502208974
2119	20-24	0.08758744004499874
2119	25-29	0.19137772526769226
2119	30-34	0.20550895907586408
2119	35-39	0.26238388802739365
2119	40-44	-0.08229721898316456
2119	45-49	0.20158955713893079
2119	50-54	0.15360384764340296
2119	55-59	0.33785599515718445
2119	60-64	0.28675228552988585
2119	65-69	0.19637960483070316
2119	70-74	0.3297795348931629
2119	75-79	0.11541540059990751
2119	80-84	0.32699421651789606
2119	85-89	0.4326010337606476
2119	90-94	0.27556070552618905
2119	95-99	0.40486504890408526
2119	100-104	0.710292754792448
2119	105-109	0.4104948688800292
2119	110-114	0.5246501138976853
2119	115-119	0.9017428113190107
2119	120-124	0.4878611208747472
2119	125-129	0.46406256728815265
2119	130-134	0.49413614439180265
2119	135-139	0.5098685800674616
2119	140-144	0.31370008714831954
2119	145-149	0.37790088620674567
2119	150-151	0.3379660507246456
1118	1	0.4464854259219706
1118	2	0.3286885561090074
1118	3	0.15955159029835642
1118	4	0.171730347160846
1118	5	0.19095570040943954
1118	6	1.3237665916463115
1118	7	0.3760688497435183
1118	8	0.45917733264067095
1118	9	0.03721879638649028
1118	10-14	0.17014766618338228
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2111	3	0.05372263880124706
2111	4	0.16936130305088426
2111	5	0.10674362270593463
2111	6	1.050425825831283
2111	7	0.39250620295597116
2111	8	-0.03284574450183442
2111	9	-0.004611733522509098
2111	10-14	-0.09045493534368632
2111	15-19	-0.06359368841430779
2111	20-24	-0.04305134603356464
2111	25-29	-0.014061071812243142
2111	30-34	-0.04807041180352911
2111	35-39	-0.09848525866001268
2111	40-44	-0.05444767043180576
2111	45-49	-0.07825300554917902
2111	50-54	-0.3652638767253862
2111	55-59	-0.3104264175981015
2111	60-64	-0.1847374949929872
2111	65-69	-0.255166479078234
2111	70-74	-0.20649037560291106
2111	75-79	-0.40342580263449435
2111	80-84	-0.18379006253945107
2111	85-89	-0.1860299649270516
2111	90-94	-0.46679295674779553
2111	95-99	-0.3893246616815773
2111	100-104	-0.31602878351192487
2111	105-109	-0.22645103586391713
2111	110-114	-0.367739892153061
2111	115-119	-1.7725955354909857
2111	120-124	-0.8678140089805879
2111	125-129	-0.6925533752533113
2111	130-134	-0.27083061578641576
2111	135-139	-0.28262515308670544
2111	140-144	-0.7465038701978415
2111	145-149	-1.0425504445648386
2111	150-151	-2.5127081754475924
2110	1	1.2961375150678265
2110	2	0.3727246101420363
2110	3	0.14551342334917194
2110	4	0.09829546579628357
2110	5	0.15023164245791065
2110	6	1.2211283276950553
2110	7	0.3616297089320639
2110	8	-0.06628642844613353
2110	9	-0.10046674406384426
2110	10-14	-0.03770076089459451
2110	15-19	-0.1629131109254658
2110	20-24	-0.14855207951813298
2110	25-29	-0.17323103181127664
2110	30-34	0.03328825814444514
2110	35-39	-0.2609815511560285
2110	40-44	-0.14907599816714168
2110	45-49	-0.1310300262390527
2110	50-54	-0.23008467401911048
2110	55-59	-0.33151129338670415
2110	60-64	-0.09488904172356882
2110	65-69	-0.09074769546020178
2110	70-74	-0.14902326216531492
2110	75-79	-0.22968835804665133
2110	80-84	-0.26873499107479404
2110	85-89	-0.21509619497666677
2110	90-94	-0.34229256796088947
2110	95-99	-0.5480855938082101
2110	100-104	0.004504375602124355
2110	105-109	-0.12711818617614057
2110	110-114	-0.49347778564949607
2110	115-119	-6.506477913423918
2110	120-124	-2.6896999060362248
2110	125-129	-0.2367269826346572
2110	130-134	-0.5723158218434747
2110	135-139	-0.7100785186987011
2110	140-144	-0.550373903144525
2110	145-149	-0.5290068322936037
2110	150-151	-0.9617360077802317
2109	1	1.2010183812928474
2109	2	0.42179372840895013
2109	3	0.04681351639757736
2109	4	0.11265335224540962
2109	5	0.05725580323283452
2109	6	1.1612532670545335
2109	7	0.5360368235565716
2109	8	0.06398432651843677
2109	9	-0.0954972035003081
2109	10-14	-0.038152681906829855
2109	15-19	-0.026341905033994806
2109	20-24	-0.30870926770655416
2109	25-29	-0.09744847083275943
2109	30-34	-0.07543841016580899
2109	35-39	-0.32483204542599964
2109	40-44	-0.09092153780814982
2109	45-49	-0.25530015292803654
2109	50-54	-0.17284737671751316
2109	55-59	-0.39982691836184614
2109	60-64	-0.2880255286291984
2109	65-69	-0.410350092450841
2109	70-74	-0.3254012931973591
2109	75-79	-0.21844162865846783
2109	80-84	-0.28097562857569613
2109	85-89	-0.3864909667694789
2109	90-94	-0.5049576891226479
2109	95-99	-0.34641290948089676
2109	100-104	-0.15625404846004187
2109	105-109	-0.3356698916660079
2109	110-114	-0.95972924698669
2109	115-119	-4.969845271723916
2109	120-124	-1.2254388456024685
2109	125-129	-0.7301157738801578
2109	130-134	-0.7085371020941551
2109	135-139	-0.8490570539417845
2109	140-144	-0.4876723976689803
2109	145-149	-0.4155048719147274
2109	150-151	-1.529242827353798
2108	1	1.0412403157801435
2108	2	0.1862887536236144
2108	3	0.07627037887542798
2108	4	0.08445495144581372
2108	5	-0.019988529146431233
2108	6	0.75501093367577
2108	7	0.044388329621718015
2108	8	-0.23930153055302839
2108	9	-0.33754322594363373
2108	10-14	-0.27663366868616635
2108	15-19	-0.3140017114943987
2108	20-24	-0.489496828471637
2108	25-29	-0.2893486571032611
2108	30-34	-5.713972779344861
2108	35-39	-0.9177330949012585
2108	40-44	-0.5778171808956998
2108	45-49	-0.21451599955018708
2108	50-54	-0.20678926940752973
2108	55-59	-0.3537851665104981
2108	60-64	-0.5019955890535286
2108	65-69	-0.4679338005967608
2108	70-74	-0.39538811796679596
2108	75-79	-0.5683300935169413
2108	80-84	-0.316011292561889
2108	85-89	-0.4967317554660369
2108	90-94	-0.6469591883730175
2108	95-99	-0.6460742151916747
2108	100-104	-0.4405880178389907
2108	105-109	-0.6288719270119643
2108	110-114	-0.8614622441244819
2108	115-119	-0.8200894678076835
2108	120-124	-0.7701719336039332
2108	125-129	-0.5557197900538853
2108	130-134	-0.7500976854388455
2108	135-139	-1.568588197460933
2108	140-144	-1.2594088021939882
2108	145-149	-1.2821188376591266
2108	150-151	-1.6563531358359214
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	1.0
16	0.0
17	3.0
18	1.0
19	25.0
20	81.0
21	227.0
22	533.0
23	944.0
24	1477.0
25	2083.0
26	2700.0
27	3776.0
28	5454.0
29	7022.0
30	9620.0
31	13589.0
32	18999.0
33	25427.0
34	35543.0
35	58078.0
36	119885.0
37	444878.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.12839980044871777	13.584617104161953	85.44284300602743	0.8441400893618993
2	47.56679243070206	0.062371142951194586	33.891652795306705	18.479183631040037
3	0.11967796232942893	0.06477003306470207	14.039237845956604	85.77631415864927
4	0.5505452810499676	0.31238880144786346	13.893172092378592	85.24389382512358
5	51.907051004401964	33.93589899073362	13.931154519175795	0.2258954856886214
6	47.98766437394965	33.745453770055725	0.4139418162530136	17.852940039741615
7	13.899702404354253	51.86093900555343	0.8618679091140499	33.37749068097827
8	51.674225391718764	14.347095410523398	33.551410214207564	0.4272689835502774
9	33.39814779028903	32.388881410867235	0.6334402616389484	33.57953053720479
10-14	14.273902607726823	46.77321292681919	23.681390076857774	15.27149438859621
15-19	33.57060133511562	20.7047939153485	22.26911015836673	23.455494591169153
20-24	27.943924610880032	27.966873992965922	10.483469648042838	33.605731748111204
25-29	26.445311302637315	41.05372580952546	12.18932040775801	20.311642480079218
30-34	16.711121654381238	16.150501034854543	40.696157910939874	26.442219399824346
35-39	34.03657241249715	17.560781878250996	20.239329270324262	28.163316438927588
40-44	8.828368741395648	33.58931267800098	30.233398680876984	27.348919899726393
45-49	9.848510089332002	22.94887565353097	32.40956517451259	34.793049082624435
50-54	19.800865466244286	31.959000302526697	16.800067168923178	31.440067062305836
55-59	18.77288774393714	31.459631343898227	42.3739949649962	7.393485947168443
60-64	24.301316590857297	25.287153810170494	24.108312554058323	26.303217044913886
65-69	15.871723349330377	39.711520136683426	24.254884739993628	20.161871773992566
70-74	17.53562018639376	13.199919503909523	37.189726886360575	32.07473342333614
75-79	27.774189808182083	28.20529701591397	23.155260166296394	20.865253009607553
80-84	3.287945443907952	29.353192589561893	37.795659874697975	29.56320209183218
85-89	26.46690131365888	31.9365840071327	27.583851204842556	14.01266347436586
90-94	13.690092717102885	49.098643694184155	23.689892809593427	13.521370779119527
95-99	9.716731059096658	41.206455146752106	34.92562774289762	14.15118605125362
100-104	15.992680719720342	39.29352686157205	17.855712090539445	26.858080328168164
105-109	17.238797516349102	32.05572888277024	34.11640214460776	16.589071456272897
110-114	39.85302799904578	14.362208418238495	18.807964848263538	26.976798734452196
115-119	13.19970626923277	46.85237630056494	16.633984010064676	23.313933420137616
120-124	3.015298255340529	20.79752434540286	46.35412682398943	29.83305057526718
125-129	23.706818312060953	33.733406010818996	6.727314162647414	35.832461514472634
130-134	20.288773060997112	47.71376443165629	24.680021376776345	7.317441130570256
135-139	37.1459437156259	8.530508826052168	36.25491572696785	18.06863173135409
140-144	14.31385745528402	43.502139676709575	18.233857135432007	23.950145732574395
145-149	26.688139404339434	29.093667088338353	30.195637537202796	14.022555970119416
150-151	39.35092697112136	24.991837110030428	18.19364907169616	17.46358684715205
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	2.0
29	4.5
30	7.5
31	16.5
32	39.0
33	122.5
34	549.0
35	5012.0
36	112091.0
37	119581.0
38	13036.5
39	1254.0
40	831.0
41	4010.5
42	37962.5
43	42274.0
44	10044.0
45	3473.5
46	6877.5
47	58965.5
48	62514.5
49	21164.5
50	117027.0
51	116800.0
52	13312.0
53	2106.0
54	622.5
55	245.0
56	140.0
57	41.0
58	82.0
59	82.0
60	22.5
61	11.5
62	8.5
63	8.0
64	3.0
65	1.0
66	1.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.225770210316027
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	2.6654334594527597E-5
140-144	0.0
145-149	2.6654334594527597E-5
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	750347.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	1.4043904519237698
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.63523252323901	1.0200822702889973
2	9.566252013771544	0.26869505977674585
3	3.5174944847363623	0.14819807007174804
4	1.875179764329688	0.10533938226661113
5	1.471110213798473	0.10330065689930558
6	1.0009558227351854	0.08434396801480774
7	0.8002466750270547	0.07867011527142176
8	0.5524394043713882	0.06206724998125056
9	0.5714528190375529	0.0722288594503135
>10	4.590542331049844	1.4882733650229847
>50	1.2952556460025906	1.3616084308553145
>100	1.752404697532917	4.7343312395436845
>500	0.15238301717677538	1.5582257582970938
>1k	0.15238301717677538	5.063096811551775
>5k	0.02857181572064538	2.295875119413701
>10k+	0.038095754294193845	81.55566364329425
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	214836	28.631553134749655	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	211158	28.141379921556293	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	108232	14.424259709174555	No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	75521	10.064810014566595	No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC	5906	0.7871025005764001	No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	5725	0.7629803277683525	No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	5534	0.7375254382305786	No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG	4844	0.6455679838794585	No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	4001	0.5332199635635246	No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC	3463	0.4615198035042453	No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG	3067	0.40874422100708074	No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	3035	0.40447952747195637	No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	2984	0.39768267215035175	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG	2832	0.37742537785851077	No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	1883	0.2509505602074773	No Hit
AGTTTGGAAACTCCGAGCCCACGAGACACTGTTTGTGATCAATTTGCCAC	1852	0.24681913834532554	No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC	1792	0.2388228379669673	No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	1750	0.2332254277021165	No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCCTCCGCGGCTATATTGA	1567	0.20883671154812372	No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	1562	0.20817035318326055	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA	1119	0.1491310020563819	No Hit
TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	1079	0.1438001351374764	No Hit
TTCCATATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	1024	0.1364701931239813	No Hit
AGTTTAGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	966	0.1287404360915683	No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA	946	0.12607500263211555	No Hit
AGTTTGGAAACTCCGAGCCCACGAGACTGTTTGTGATCAATTTGCCACTG	892	0.11887833229159309	No Hit
TTCCAGATCTCGATTCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	842	0.11221474864296119	No Hit
TTCCAGATCTCGATGCCCACAGGCAGAGAATTTTCCGGCACATATTCGCC	837	0.111548390278098	No Hit
TTCCACATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	790	0.10528462164838401	No Hit
AGTTTGTAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	761	0.10141974313217751	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10-11	0.0	0.0	0.0
12-13	0.0	0.0	0.0
14-15	0.0	0.0	0.0
16-17	0.0	0.0	1.33271672972638E-4
18-19	0.0	0.0	0.001066173383781104
20-21	0.0	0.0	0.03218510902289208
22-23	0.0	0.0	0.048310981452581274
24-25	0.0	0.0	0.05024342071068452
26-27	0.0	0.0	0.05497456510121317
28-29	0.0	0.0	0.07729757032413004
30-31	0.0	0.0	0.0778306570160206
32-33	0.0	0.0	0.0894252925646401
34-35	0.0	0.0	0.09588896870381303
36-37	0.0	0.0	0.09708841376056679
38-39	0.0	0.0	0.1475983778171966
40-41	0.0	0.0	0.16932166051173658
42-43	0.0	0.0	0.21956508122242108
44-45	0.0	0.0	0.3535031125599223
46-47	0.0	0.0	0.4598539075920874
48-49	0.0	0.0	0.6612274054537434
50-51	0.0	0.0	1.0026694316096418
52-53	0.0	0.0	1.1469360176025227
54-55	0.0	0.0	1.1525334278673733
56-57	0.0	0.0	1.15766438727682
58-59	0.0	0.0	1.1587971964970873
60-61	0.0	0.0	1.169592202007871
62-63	0.0	0.0	1.178787947442983
64-65	0.0	0.0	1.1790544907889284
66-67	0.0	0.0	1.1803872075186548
68-69	0.0	0.0	1.186517704475396
70-71	0.0	0.0	1.1866509761483688
72-73	0.0	0.0	1.1868508836578275
74-75	0.0	0.0	1.1870507911672865
76-77	0.0	0.0	1.1882502362240404
78-79	0.0	0.0	1.1883168720605268
80-81	0.0	0.0	1.1888499587524173
82-83	0.0	0.0	1.1889165945889035
84-85	0.0	0.0	1.1890498662618763
86-87	0.0	0.0	1.1890498662618763
88-89	0.0	0.0	1.1890498662618763
90-91	0.0	0.0	1.1891831379348488
92-93	0.0	0.0	1.1893164096078215
94-95	0.0	0.0	1.1893164096078215
96-97	0.0	0.0	1.189449681280794
98-99	0.0	0.0	1.1897162246267394
100-101	0.0	0.0	1.1897162246267394
102-103	0.0	0.0	1.1939809181618637
104-105	0.0	0.0	1.1979124325145567
106-107	0.0	0.0	1.1981123400240155
108-109	0.0	0.0	1.1983788833699607
110-111	0.0	0.0	1.1986454267159061
112-113	0.0	0.0	1.1987786983888786
114-115	0.0	0.0	1.1989119700618514
116-117	0.0	0.0	1.1991785134077966
118-119	0.0	0.0	1.1997782359361735
120-121	0.0	0.0	1.200111415118605
122-123	0.0	0.0	1.216970281749644
124-125	0.0	0.0	1.2333626975252783
126-127	0.0	0.0	1.2342289633996004
128-129	0.0	0.0	1.234295599236087
130-131	0.0	0.0	1.2346287784185184
132-133	0.0	0.0	1.234762050091491
134-135	0.0	0.0	1.234762050091491
136-137	0.0	0.0	1.234762050091491
138-139	0.0	0.0	1.234762050091491
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGGA	10	0.006632053	148.27809	1
TGCCGCG	20	1.9059044E-6	148.27809	1
TTTCAGA	30	5.5661076E-10	148.27809	1
ACCCGCA	20	1.9059044E-6	148.27809	1
TACACGC	20	1.9059044E-6	148.27809	1
CGGGTGT	30	5.5661076E-10	148.27809	1
TGCCAGA	10	0.006632053	148.27809	1
TCCCGCA	12435	0.0	148.27809	1
AGTTTTG	15	1.12507936E-4	148.27809	1
AGTTTGC	30	5.5661076E-10	148.27809	1
TCCCCAG	10	0.006632053	148.27809	1
TTCCGCA	25	3.246896E-8	148.27808	1
TGCCGAG	65	0.0	148.27808	1
CGTGTGT	65	0.0	148.27808	1
CGTGTGG	55	0.0	148.27808	1
TCCCGGA	390	0.0	148.27808	1
TCCCGCG	35	9.094947E-12	148.27808	1
TCCCGAA	180	0.0	148.27808	1
AGTTTGT	230	0.0	148.27808	1
AGTTTGG	9070	0.0	148.27808	1
>>END_MODULE