FastQCFastQC Report
Wed 17 Jun 2015
000000000-AG1W8 l01n01 mis17.3410000000d27f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG1W8 l01n01 mis17.3410000000d27f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750347
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG21483628.631553134749655No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC21115828.141379921556293No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG10823214.424259709174555No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA7552110.064810014566595No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC59060.7871025005764001No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG57250.7629803277683525No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC55340.7375254382305786No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG48440.6455679838794585No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG40010.5332199635635246No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC34630.4615198035042453No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG30670.40874422100708074No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG30350.40447952747195637No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG29840.39768267215035175No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG28320.37742537785851077No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC18830.2509505602074773No Hit
AGTTTGGAAACTCCGAGCCCACGAGACACTGTTTGTGATCAATTTGCCAC18520.24681913834532554No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC17920.2388228379669673No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA17500.2332254277021165No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCCTCCGCGGCTATATTGA15670.20883671154812372No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG15620.20817035318326055No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA11190.1491310020563819No Hit
TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG10790.1438001351374764No Hit
TTCCATATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC10240.1364701931239813No Hit
AGTTTAGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA9660.1287404360915683No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA9460.12607500263211555No Hit
AGTTTGGAAACTCCGAGCCCACGAGACTGTTTGTGATCAATTTGCCACTG8920.11887833229159309No Hit
TTCCAGATCTCGATTCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC8420.11221474864296119No Hit
TTCCAGATCTCGATGCCCACAGGCAGAGAATTTTCCGGCACATATTCGCC8370.111548390278098No Hit
TTCCACATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC7900.10528462164838401No Hit
AGTTTGTAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA7610.10141974313217751No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGGA100.006632053148.278091
TGCCGCG201.9059044E-6148.278091
TTTCAGA305.5661076E-10148.278091
ACCCGCA201.9059044E-6148.278091
TACACGC201.9059044E-6148.278091
CGGGTGT305.5661076E-10148.278091
TGCCAGA100.006632053148.278091
TCCCGCA124350.0148.278091
AGTTTTG151.12507936E-4148.278091
AGTTTGC305.5661076E-10148.278091
TCCCCAG100.006632053148.278091
TTCCGCA253.246896E-8148.278081
TGCCGAG650.0148.278081
CGTGTGT650.0148.278081
CGTGTGG550.0148.278081
TCCCGGA3900.0148.278081
TCCCGCG359.094947E-12148.278081
TCCCGAA1800.0148.278081
AGTTTGT2300.0148.278081
AGTTTGG90700.0148.278081
Produced by FastQC (version 0.11.2)