##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AG1W8 l01n01 mis16.3410000000d262.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	756950
Sequences flagged as poor quality	0
Sequence length	151
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07540656582337	34.0	34.0	34.0	31.0	34.0
2	33.43212497522954	34.0	34.0	34.0	33.0	34.0
3	33.424375454125105	34.0	34.0	34.0	33.0	34.0
4	33.440027742915646	34.0	34.0	34.0	34.0	34.0
5	33.67558491313825	34.0	34.0	34.0	34.0	34.0
6	36.36540062091287	38.0	38.0	38.0	34.0	38.0
7	36.69097034150208	38.0	38.0	38.0	34.0	38.0
8	36.797180791333645	38.0	37.0	38.0	34.0	38.0
9	37.35241165202457	38.0	38.0	38.0	36.0	38.0
10-14	37.24704643635643	38.0	38.0	38.0	36.4	38.0
15-19	37.17684893321884	38.0	38.0	38.0	36.2	38.0
20-24	37.45811374595416	38.0	38.0	38.0	36.4	38.0
25-29	37.17940207411322	38.0	38.0	38.0	36.2	38.0
30-34	36.81294458022326	38.0	37.8	38.0	35.2	38.0
35-39	36.99462897153048	38.0	37.8	38.0	35.8	38.0
40-44	36.83354250611005	38.0	38.0	38.0	35.2	38.0
45-49	36.978455908580486	38.0	38.0	38.0	36.0	38.0
50-54	36.920977607503794	38.0	37.8	38.0	35.6	38.0
55-59	36.51792482990951	38.0	37.2	38.0	34.0	38.0
60-64	36.44460241759694	38.0	37.0	38.0	33.8	38.0
65-69	36.61096743510139	38.0	37.2	38.0	34.6	38.0
70-74	36.68208573882026	38.0	37.2	38.0	34.8	38.0
75-79	36.283303256489866	38.0	37.0	38.0	33.6	38.0
80-84	36.53721672501486	38.0	37.0	38.0	34.0	38.0
85-89	36.11831190963736	38.0	37.0	38.0	32.8	38.0
90-94	36.46171556906005	38.0	37.0	38.0	34.2	38.0
95-99	36.277807781227295	38.0	37.0	38.0	33.8	38.0
100-104	35.827594689213285	38.0	36.4	38.0	31.6	38.0
105-109	36.061005086201206	38.0	37.0	38.0	32.4	38.0
110-114	36.03738423938173	38.0	37.0	38.0	32.8	38.0
115-119	35.669222009379745	38.0	36.8	38.0	30.2	38.0
120-124	35.77374859634058	38.0	36.6	38.0	30.6	38.0
125-129	35.7229167051985	38.0	36.8	38.0	30.8	38.0
130-134	35.138575335226896	38.0	35.8	38.0	28.0	38.0
135-139	34.92208626725675	37.8	35.6	38.0	26.8	38.0
140-144	35.41291472356166	38.0	36.4	38.0	29.4	38.0
145-149	35.06639804478499	37.8	35.4	38.0	27.6	38.0
150-151	33.17475328621441	36.0	32.5	37.5	23.5	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.5304983435907467
1101	2	0.3392892412971946
1101	3	0.2004353466530162
1101	4	0.12565611130229115
1101	5	0.18294253763916402
1101	6	1.1124176991216785
1101	7	-0.08759864311021204
1101	8	0.30343801216193356
1101	9	0.1582929761627696
1101	10-14	0.24258969440322176
1101	15-19	0.3100920868010846
1101	20-24	0.15965377049275276
1101	25-29	0.2803271150706408
1101	30-34	0.251902628031651
1101	35-39	0.14833360440385235
1101	40-44	0.15432791004765534
1101	45-49	0.25682150602152376
1101	50-54	0.23589479486341958
1101	55-59	0.37136201037739625
1101	60-64	0.45081466296141315
1101	65-69	0.35230068036672435
1101	70-74	0.17506904899679654
1101	75-79	0.296576800872252
1101	80-84	0.4215240358447261
1101	85-89	0.505764295080013
1101	90-94	0.3775525152629555
1101	95-99	0.418751493887072
1101	100-104	0.6262817027184724
1101	105-109	0.5049118413097133
1101	110-114	0.40836904768833193
1101	115-119	0.7593697895988143
1101	120-124	0.3822954125412039
1101	125-129	0.3478161936222648
1101	130-134	0.3705279186244965
1101	135-139	0.5147297218379663
1101	140-144	0.333805922478696
1101	145-149	0.6396910525404067
1101	150-151	0.5538960161639324
1102	1	0.46919569121666527
1102	2	0.3067684552049812
1102	3	0.12364361986129069
1102	4	0.18514728150774573
1102	5	0.21329452691972506
1102	6	1.1392566670320718
1102	7	0.39168827367670644
1102	8	0.268365346732125
1102	9	0.25664383165648275
1102	10-14	0.2923895638459513
1102	15-19	0.31692160648774603
1102	20-24	0.17555478746519526
1102	25-29	0.32858297296935746
1102	30-34	0.22730134307322203
1102	35-39	0.25098219919530607
1102	40-44	0.24650667044070218
1102	45-49	0.35510381894669507
1102	50-54	0.35982096128957863
1102	55-59	0.5169608559762366
1102	60-64	0.4837176726501369
1102	65-69	0.4543573009947721
1102	70-74	0.26996391603451286
1102	75-79	0.26154587941275764
1102	80-84	0.4644651820001613
1102	85-89	0.2867676964405561
1102	90-94	0.473529633382924
1102	95-99	0.5546095643880022
1102	100-104	0.7516455452251734
1102	105-109	0.48106194781697553
1102	110-114	0.4602724353060026
1102	115-119	0.7915562803567369
1102	120-124	0.6323739186911439
1102	125-129	0.6159330767391467
1102	130-134	0.7700453720705198
1102	135-139	0.7816348273144982
1102	140-144	0.5734754064338929
1102	145-149	0.7445761277826293
1102	150-151	1.2200645037609945
1103	1	0.8316641177986845
1103	2	0.35015235185910143
1103	3	0.23390083252813554
1103	4	0.30134261801288886
1103	5	0.17539825795633135
1103	6	1.1846755338795134
1103	7	0.6122026990104104
1103	8	0.3702552812382436
1103	9	0.2602495202879851
1103	10-14	0.25277702492186904
1103	15-19	0.32842490525438706
1103	20-24	0.18302786516202474
1103	25-29	0.2649053425103176
1103	30-34	0.3681583346186841
1103	35-39	0.22454082645444373
1103	40-44	0.37442462168051094
1103	45-49	0.29261776799248196
1103	50-54	0.26967255547852886
1103	55-59	0.46605874603798725
1103	60-64	0.5614585204426206
1103	65-69	0.34419921467490155
1103	70-74	0.32423339870331347
1103	75-79	0.38190426457320115
1103	80-84	0.4165613646471158
1103	85-89	0.40827665220080434
1103	90-94	0.37637704414772344
1103	95-99	0.48564445032822334
1103	100-104	0.7000732792811419
1103	105-109	0.5571314033184365
1103	110-114	0.519143770511242
1103	115-119	0.9987567391062484
1103	120-124	0.8532336097745841
1103	125-129	0.5793870926027935
1103	130-134	0.7747817716434113
1103	135-139	0.8633041906533947
1103	140-144	0.7878669529252704
1103	145-149	0.9254028741859699
1103	150-151	1.027658587818074
2116	1	0.623359680537444
2116	2	0.2697002620740676
2116	3	-0.060043424801364154
2116	4	-0.35179194651162504
2116	5	-0.01580407482695989
2116	6	0.9935310685887018
2116	7	-0.19519801077055376
2116	8	0.154921751999332
2116	9	0.0715721089540935
2116	10-14	0.18070396721260806
2116	15-19	0.24234682934850582
2116	20-24	0.07412757356411248
2116	25-29	0.25963921985835725
2116	30-34	0.1811659523315612
2116	35-39	0.07410686546979406
2116	40-44	0.05290288746409999
2116	45-49	0.1841670344768076
2116	50-54	0.1723252375011839
2116	55-59	0.392315939916692
2116	60-64	0.40570219683674935
2116	65-69	0.35690411306772063
2116	70-74	0.22566254493175109
2116	75-79	0.2342922480267191
2116	80-84	0.3732218678230481
2116	85-89	0.4573699861369249
2116	90-94	0.42481267786457266
2116	95-99	0.39627588413097925
2116	100-104	0.7790242501439479
2116	105-109	0.40156540951207376
2116	110-114	0.3421504388355743
2116	115-119	0.83275371010469
2116	120-124	0.6004684026044416
2116	125-129	0.4587186326466508
2116	130-134	0.5512546575585588
2116	135-139	0.7739022602300096
2116	140-144	0.6543443145292756
2116	145-149	0.7440501311311962
2116	150-151	1.4877397379344899
1117	1	0.4155525015222601
1117	2	0.2991250204726015
1117	3	0.1694779741862078
1117	4	0.12123612527852856
1117	5	0.1265222022093937
1117	6	1.1466856935311824
1117	7	0.26307396345862344
1117	8	0.2953405278852088
1117	9	0.16086111033467887
1117	10-14	0.21093176288680127
1117	15-19	0.25586776814280654
1117	20-24	0.12089241479742441
1117	25-29	0.2322121604934253
1117	30-34	0.3959574847751881
1117	35-39	0.26677874766031806
1117	40-44	0.19922134121467394
1117	45-49	0.4019944619963596
1117	50-54	0.27157788361254376
1117	55-59	0.562263477882631
1117	60-64	0.5542999879089976
1117	65-69	0.4407632939236805
1117	70-74	0.3732451496257241
1117	75-79	0.3739038448974057
1117	80-84	0.4293594656141124
1117	85-89	0.5771007730674285
1117	90-94	0.3943764146340172
1117	95-99	-0.08233341177330544
1117	100-104	0.5974347425507602
1117	105-109	0.5499100942796744
1117	110-114	0.6315940651586303
1117	115-119	1.0638701390048269
1117	120-124	0.7460183335754493
1117	125-129	0.6057407219241426
1117	130-134	0.5986393791416162
1117	135-139	0.6175005114955425
1117	140-144	0.5445955800608004
1117	145-149	-0.31364752188306966
1117	150-151	0.3725717673868587
2117	1	-29.927372026779626
2117	2	-10.406007054999101
2117	3	-2.190362374144705
2117	4	-0.8173153986692228
2117	5	-0.13524122304460207
2117	6	1.0849832261872052
2117	7	-0.04616986818143687
2117	8	0.05991237113629211
2117	9	0.19672032659011762
2117	10-14	0.37409083400436316
2117	15-19	0.4287348218438112
2117	20-24	0.21580562234459677
2117	25-29	0.3599889384324655
2117	30-34	0.4161798360088653
2117	35-39	0.288187165657412
2117	40-44	0.13772990434964782
2117	45-49	0.43942407012409035
2117	50-54	0.20722955269817334
2117	55-59	0.4706919249073138
2117	60-64	0.4283693638161097
2117	65-69	0.37901293107897516
2117	70-74	0.44255110027884115
2117	75-79	0.3006742367696873
2117	80-84	0.5227693349900235
2117	85-89	0.35523865405437505
2117	90-94	0.3510550793655156
2117	95-99	-0.08804081380523598
2117	100-104	0.8829552069919728
2117	105-109	0.604435203133086
2117	110-114	0.6047200448393255
2117	115-119	0.9985128095418361
2117	120-124	0.717272905418703
2117	125-129	0.37992388592994786
2117	130-134	0.6254820496786238
2117	135-139	0.3585086392168719
2117	140-144	0.5794309937037667
2117	145-149	0.6494354969848501
2117	150-151	1.193927355195271
1116	1	-0.3524797854003161
1116	2	-0.23276136534392577
1116	3	-0.7299446022614191
1116	4	-1.0844811811625874
1116	5	-0.2651417535485763
1116	6	1.012593305762799
1116	7	-0.6247554119480156
1116	8	0.1483374374492925
1116	9	0.04642755470683113
1116	10-14	0.17901609925634432
1116	15-19	0.2876016653451501
1116	20-24	0.08135476027563016
1116	25-29	0.057380914559786333
1116	30-34	0.20822366890010358
1116	35-39	0.19627980491470964
1116	40-44	0.004997609919939805
1116	45-49	0.29299966694105706
1116	50-54	0.14357040150454026
1116	55-59	0.37763595673754935
1116	60-64	0.3299933028081554
1116	65-69	0.3295580239171727
1116	70-74	0.2832012329048368
1116	75-79	0.18621760546994892
1116	80-84	0.3521083270324681
1116	85-89	0.3873878582984034
1116	90-94	0.4296142968721526
1116	95-99	-0.2035760923198211
1116	100-104	0.6463343847108121
1116	105-109	0.4479354683850687
1116	110-114	0.46800346658999104
1116	115-119	0.9200107538389233
1116	120-124	0.5942909425539824
1116	125-129	0.33033774534639804
1116	130-134	0.3924739594929463
1116	135-139	0.5313261458481762
1116	140-144	0.535467001661317
1116	145-149	0.42247601422623404
1116	150-151	0.4106868047974004
2118	1	0.7938458609463517
2118	2	0.3601432780170768
2118	3	0.12067180575721892
2118	4	0.1560143239392815
2118	5	0.1148473373008585
2118	6	1.156680218063073
2118	7	0.5627189556493519
2118	8	0.200468617934213
2118	9	0.05203438645983738
2118	10-14	0.2075485519286815
2118	15-19	0.21271075685076823
2118	20-24	0.10189810569378466
2118	25-29	0.2120467295471471
2118	30-34	0.2907773328982728
2118	35-39	0.04662112598823143
2118	40-44	0.010604031554507287
2118	45-49	0.2555853816954823
2118	50-54	0.10634189991328213
2118	55-59	0.3635176293646012
2118	60-64	0.2673293579608824
2118	65-69	0.3517269763106228
2118	70-74	0.3742143074807771
2118	75-79	0.336140708262036
2118	80-84	0.3124974474104363
2118	85-89	0.21110603310729203
2118	90-94	0.21876881719762054
2118	95-99	0.44106119892989426
2118	100-104	0.7517928561173548
2118	105-109	0.4117646622562603
2118	110-114	0.5527571084417531
2118	115-119	0.8521928505284393
2118	120-124	0.5544430144723549
2118	125-129	0.35960020884585475
2118	130-134	0.4584511487051941
2118	135-139	0.4464554151811555
2118	140-144	0.3836857547372077
2118	145-149	0.22124063494869262
2118	150-151	1.0163171670960338
1119	1	1.2959755211947872
1119	2	0.3525345228260619
1119	3	0.06718888057072547
1119	4	-0.06215285971563844
1119	5	0.11106679729326885
1119	6	1.2299026947783176
1119	7	0.4317638697224524
1119	8	0.17615478543841334
1119	9	0.07202195033950431
1119	10-14	0.2718886362021564
1119	15-19	0.18463129551939517
1119	20-24	0.006276411257182701
1119	25-29	0.14888452642655636
1119	30-34	0.24962848288436845
1119	35-39	0.1656237410223511
1119	40-44	0.19801443772671234
1119	45-49	0.11570387986657948
1119	50-54	0.016023713760993985
1119	55-59	0.3499808210857509
1119	60-64	0.29615640662405696
1119	65-69	0.18962437933221565
1119	70-74	0.25317494807283936
1119	75-79	0.3855995442979747
1119	80-84	0.25993495742729067
1119	85-89	0.4172124116400653
1119	90-94	0.30430802343637
1119	95-99	0.2799508542282254
1119	100-104	0.6454690990700485
1119	105-109	0.3211984637530634
1119	110-114	0.3497139106749785
1119	115-119	0.7782286698009813
1119	120-124	0.5791063972999524
1119	125-129	0.27705926398473224
1119	130-134	0.4897889547138945
1119	135-139	0.4121529232910177
1119	140-144	0.5531267785159599
1119	145-149	0.5256120428270705
1119	150-151	0.9475033761892035
2119	1	0.7832927269267351
2119	2	0.3688298651348063
2119	3	0.2788853174272319
2119	4	0.24332043990635555
2119	5	0.1960084550630441
2119	6	1.275577936116207
2119	7	0.6648689428401582
2119	8	0.32620821780516707
2119	9	0.02642624573076091
2119	10-14	0.22544810877167976
2119	15-19	0.1763745672729513
2119	20-24	0.11911083516142895
2119	25-29	0.1027726488397036
2119	30-34	0.19238737748699464
2119	35-39	0.15503082529763645
2119	40-44	-0.06417028326761454
2119	45-49	0.19219572888139425
2119	50-54	0.09973413276660636
2119	55-59	0.3478166572768018
2119	60-64	0.16312735226478026
2119	65-69	0.21769416567413202
2119	70-74	0.21454059371224332
2119	75-79	0.009544280547046924
2119	80-84	0.19379766887517036
2119	85-89	0.26088026829857114
2119	90-94	0.21370029144659952
2119	95-99	0.2692285383634001
2119	100-104	0.7053140636303183
2119	105-109	0.328536236867393
2119	110-114	0.38091275351753495
2119	115-119	0.8059971343036452
2119	120-124	0.45031058337919205
2119	125-129	0.41041064603737
2119	130-134	0.45450860303918716
2119	135-139	0.2725574491348439
2119	140-144	0.25054326240110925
2119	145-149	0.2968554874200464
2119	150-151	0.44179521378336517
1118	1	0.29998558293092614
1118	2	0.3182730570457153
1118	3	0.21115741325743187
1118	4	0.2891403939042192
1118	5	0.2387198485635338
1118	6	1.2741318292181631
1118	7	0.4481350948294107
1118	8	0.5521424939546549
1118	9	0.18779811155025783
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2111	6	0.9641863456483861
2111	7	0.555119824205029
2111	8	0.024808979903873762
2111	9	0.00816496797024513
2111	10-14	-0.12460393932267522
2111	15-19	-0.126881799527645
2111	20-24	-0.023206359222221806
2111	25-29	0.00290818692590733
2111	30-34	-0.10700119514635986
2111	35-39	-0.19636088270998897
2111	40-44	-0.21650627667362699
2111	45-49	-0.2303251913671076
2111	50-54	-0.4331967736636315
2111	55-59	-0.2767178667347423
2111	60-64	-0.20308318650094748
2111	65-69	-0.39239295335916324
2111	70-74	-0.29732982453111134
2111	75-79	-0.38389170911239034
2111	80-84	-0.1894987496135414
2111	85-89	-0.46881711813043125
2111	90-94	-0.7644060100826735
2111	95-99	-0.5124095404191209
2111	100-104	-0.4705078518130179
2111	105-109	-0.4496217821755195
2111	110-114	-0.6235988649247446
2111	115-119	-2.4255286572351125
2111	120-124	-1.1134244201947538
2111	125-129	-0.8524628642374452
2111	130-134	-0.577853631128022
2111	135-139	-0.5512951970901838
2111	140-144	-1.0526286226655301
2111	145-149	-1.368812531688988
2111	150-151	-2.6740652400231255
2110	1	1.3812379317265986
2110	2	0.42660290350712415
2110	3	0.17001426166761746
2110	4	0.20462491413001516
2110	5	0.2080750872011734
2110	6	1.1856330825549009
2110	7	0.40507271081633434
2110	8	-0.09996793527928105
2110	9	0.011808421068252528
2110	10-14	-0.01769346219736434
2110	15-19	-0.06552109899882197
2110	20-24	-0.1354757054562299
2110	25-29	-0.08445581100896504
2110	30-34	0.12444335340433099
2110	35-39	-0.1980349217987012
2110	40-44	-0.1076173028000511
2110	45-49	-0.08031196562439646
2110	50-54	-0.2217196972220279
2110	55-59	-0.3824531117987249
2110	60-64	-0.045837019841727056
2110	65-69	-0.0896203277783485
2110	70-74	-0.11392818475147948
2110	75-79	-0.1315075698054784
2110	80-84	-0.09118112782516619
2110	85-89	-0.12166654862676296
2110	90-94	-0.21625108086428924
2110	95-99	-0.5039767218805622
2110	100-104	0.04064777003059561
2110	105-109	-0.11406195613918158
2110	110-114	-0.5782415255405127
2110	115-119	-6.394335450911402
2110	120-124	-2.702446788165723
2110	125-129	-0.32185185617026946
2110	130-134	-0.47954546388664454
2110	135-139	-0.6425578146611883
2110	140-144	-0.4724235227341538
2110	145-149	-0.5897337976209585
2110	150-151	-0.929746599480751
2109	1	1.227127973220373
2109	2	0.4259929450008997
2109	3	0.10817608739375828
2109	4	0.11441537056154516
2109	5	0.0642203154169394
2109	6	1.0537139954179722
2109	7	0.7197532087416434
2109	8	-0.07439532117139436
2109	9	-0.08481813494834256
2109	10-14	-0.006639935226409932
2109	15-19	-0.051419024310021655
2109	20-24	-0.33569437765540755
2109	25-29	-0.11158029233676814
2109	30-34	-0.20230477937575841
2109	35-39	-0.48035898818874045
2109	40-44	-0.09622394180420457
2109	45-49	-0.36844516064514465
2109	50-54	-0.22170890884028438
2109	55-59	-0.8191157674003762
2109	60-64	-0.3843075592608116
2109	65-69	-0.4291178381517895
2109	70-74	-0.47176428433653683
2109	75-79	-0.3161565324610791
2109	80-84	-0.34155374193304766
2109	85-89	-0.5064690382533215
2109	90-94	-0.7009141514037154
2109	95-99	-0.326448506112925
2109	100-104	-0.22482940839263676
2109	105-109	-0.34289556609768823
2109	110-114	-1.0093568782375968
2109	115-119	-5.2292487289197
2109	120-124	-1.4243194022736105
2109	125-129	-0.7656838063777371
2109	130-134	-0.8776794887829098
2109	135-139	-0.9350221300138912
2109	140-144	-0.5471459293731584
2109	145-149	-0.4424645030151524
2109	150-151	-1.8016687986508764
2108	1	0.8185933049597658
2108	2	0.13981004003975528
2108	3	-0.036438975367751425
2108	4	-0.05574511957593131
2108	5	-0.06410783760158978
2108	6	0.7496210486158219
2108	7	-0.06535677332649925
2108	8	-0.41223991172728347
2108	9	-0.5877254870105091
2108	10-14	-0.34529606194487883
2108	15-19	-0.48530473859573675
2108	20-24	-0.570139625712784
2108	25-29	-0.44580862467388727
2108	30-34	-5.539947397427156
2108	35-39	-0.9860730945844409
2108	40-44	-0.7830424713917665
2108	45-49	-0.32980780260569986
2108	50-54	-0.23140872952169644
2108	55-59	-0.41854965861377735
2108	60-64	-0.6707276429428148
2108	65-69	-0.5145262661254861
2108	70-74	-0.4849797655080863
2108	75-79	-0.6758210871532526
2108	80-84	-0.5015182368523554
2108	85-89	-0.8020206222341955
2108	90-94	-0.804383129287757
2108	95-99	-0.7329864021081391
2108	100-104	-0.6369095040292123
2108	105-109	-0.7064629898872852
2108	110-114	-0.9313644096183538
2108	115-119	-1.0120118490631569
2108	120-124	-1.120091129709344
2108	125-129	-0.7055878709324332
2108	130-134	-0.8557811624230851
2108	135-139	-1.658150223259561
2108	140-144	-1.670490639474778
2108	145-149	-1.514255118675635
2108	150-151	-1.9243228632055676
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	2.0
18	6.0
19	19.0
20	100.0
21	340.0
22	733.0
23	1220.0
24	1823.0
25	2474.0
26	3095.0
27	4267.0
28	5590.0
29	7773.0
30	10483.0
31	14359.0
32	20495.0
33	27481.0
34	38578.0
35	64658.0
36	131239.0
37	422213.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.14054291959351908	21.49280611962484	77.11040517133141	1.2562457894502355
2	51.518462249818356	0.053504194464627786	33.477772640200804	14.950260915516218
3	0.15892727392826475	0.06394081511328358	22.265010899002576	77.51212101195587
4	0.6658299755598124	0.27993922980381797	21.985467996565163	77.0687627980712
5	44.31309861945967	33.415417134553145	22.07305634454059	0.19842790144659492
6	55.46363696413237	29.579100336878263	0.44481141422815246	14.512451284761212
7	21.796948279278684	47.99709359931303	1.0736508355901975	29.132307285818086
8	43.8916705198494	22.585507629301805	33.067705925094124	0.4551159257546734
9	29.155030054825286	37.14115859700112	0.5610674417068499	33.14274390646674
10-14	12.800924763854942	43.32793447387542	23.72420899663122	20.146931765638417
15-19	32.403302728053376	20.142228680890415	23.572415615298237	23.882052975757976
20-24	25.27252790805205	24.43799458352599	17.76936389457692	32.520113613845034
25-29	28.213461919545544	37.1177224387344	15.087865777131912	19.58094986458815
30-34	16.039791267587027	17.00447849924037	39.84744038575864	27.108289847413964
35-39	32.02473082766365	15.114340445207741	19.74481801968426	33.11611070744435
40-44	11.74698460928727	32.95668141885197	29.45734856991875	25.838985401942004
45-49	11.675090825021469	22.450518528304382	34.82935464693837	31.04503599973578
50-54	19.237360459739744	34.61779509875157	18.40628839421362	27.738556047295067
55-59	22.154805469317658	27.163247242222077	43.31747143140234	7.36447585705793
60-64	20.601307880309136	29.1007860492767	20.802959244335824	29.49494682607834
65-69	18.594940220622235	38.827927868419316	22.302239249620186	20.274892661338264
70-74	15.620609023053042	13.423264416407953	35.476134487086334	35.47999207345267
75-79	24.13971860756985	31.803844375454126	22.94901908976815	21.107417927207877
80-84	5.152519981504724	30.382825814122466	34.31426117973446	30.15039302463835
85-89	26.372785520840214	35.33847678182179	25.80466345201136	12.48407424532664
90-94	13.547922584054428	49.993500231190964	22.040795296915253	14.417781887839357
95-99	10.793236012946695	45.61075368254178	29.632393156747472	13.963617147764054
100-104	18.815324658167647	38.53567606843252	16.00428033555717	26.64471893784266
105-109	14.687522293414359	33.24367527577779	33.91029790607041	18.158504524737435
110-114	38.6235022128278	13.32122333047097	21.7580817755466	26.297192681154634
115-119	13.561186339916773	46.0424070282053	17.953233370764252	22.44317326111368
120-124	4.922993592707576	20.839896954884736	43.89431270229209	30.342796750115596
125-129	21.625206420503336	34.64574938899531	7.779034282317195	35.950009908184164
130-134	19.773168637294404	51.547235616619325	21.516691987581744	7.162903758504524
135-139	33.344606645088845	14.229103639606313	35.460228548781295	16.96606116652355
140-144	11.8660149283308	42.246040029064005	23.89214611268908	21.99579892991611
145-149	25.28476121276174	32.46145716361715	29.126520906268578	13.127260717352533
150-151	41.1844243345003	25.27808970209393	18.102648787898804	15.434837175506969
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.5
28	2.0
29	6.0
30	10.0
31	12.0
32	32.5
33	105.5
34	494.0
35	4470.5
36	97810.5
37	104931.0
38	12243.0
39	1428.5
40	1270.0
41	6798.5
42	65563.0
43	72761.0
44	16795.5
45	5162.5
46	6152.5
47	44704.0
48	47224.5
49	19683.5
50	114460.5
51	114258.5
52	14410.5
53	2840.5
54	1349.0
55	786.0
56	177.0
57	63.5
58	58.5
59	88.0
60	74.0
61	62.0
62	274.5
63	279.0
64	49.0
65	28.5
66	20.5
67	2.5
68	2.0
69	2.0
70	1.5
71	0.5
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.1469053438139905
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	756950.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	1.5874525210116939
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.27622307169827	1.1632252504538265
2	9.397031529254356	0.298346827822824
3	3.4836895145149773	0.16590575106716415
4	2.124808336811147	0.1349212940375008
5	1.3473810260947672	0.10694517033187304
6	0.8406651619430124	0.08007096183918834
7	0.6199988590295259	0.06889531262535563
8	0.5856493488727411	0.0743752428237512
9	0.5016817555469809	0.07167563708297645
>10	4.605179754383258	1.6321705484787068
>50	1.312149578624926	1.535563978452737
>100	1.5461635162140848	5.140172387868878
>500	0.15043753130731635	1.6149027774085636
>1k	0.15043753130731635	5.2436577778112605
>5k	0.025072921884552724	2.128482193391849
>10k+	0.03343056251273697	80.54068888850355
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	205126	27.09901578704009	No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	188646	24.921857454257214	No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	130693	17.265737499174318	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	82594	10.911420833608561	No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC	5602	0.7400753021996169	No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	5427	0.7169562058260124	No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	5014	0.6623951383843054	No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG	3946	0.52130259594425	No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	3691	0.4876147697998547	No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	3053	0.40332914987779905	No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC	2900	0.3831164541911619	No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCCTCCGCGGCTATATTGA	2828	0.3736045973974503	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCGTTCGCAATTTTTCCGTCGGCAC	2582	0.341105753352269	No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	2396	0.3165334566351807	No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG	2390	0.31574080190237136	No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	2232	0.29486756060505975	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG	2060	0.2721447915978598	No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	1854	0.24493031243807387	No Hit
AGTTTAGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	1649	0.21784794240042277	No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA	1636	0.21613052381266926	No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC	1512	0.19974899266794371	No Hit
AGTTTGTAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	1366	0.18046106083625074	No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	1181	0.15602087324129732	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA	1150	0.15192549045511594	No Hit
TTCCATATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	1097	0.1449237069819671	No Hit
TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	970	0.12814584847083693	No Hit
TTCCAGATCTCGATGCCGACAGTCCCGGAGCCGCTCAGCGAGCCGTCTTC	886	0.11704868221150672	No Hit
TTCCAGATCTCGATGCCCACAGGCAGAGAATTTTCCGGCACATATTCGCC	869	0.11480282713521368	No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA	835	0.11031111698262765	No Hit
TTCCAGATCTCGATTCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	817	0.10793315278419975	No Hit
TTCCACATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	801	0.10581940683004161	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10-11	0.0	0.0	2.6421824426976683E-4
12-13	0.0	0.0	3.3027280533720854E-4
14-15	0.0	0.0	3.9632736640465025E-4
16-17	0.0	0.0	3.9632736640465025E-4
18-19	0.0	0.0	0.0013871457824162759
20-21	0.0	0.0	0.010568729770790673
22-23	0.0	0.0	0.02166589603012088
24-25	0.0	0.0	0.030451152652090627
26-27	0.0	0.0	0.03362177158332783
28-29	0.0	0.0	0.041350155228218506
30-31	0.0	0.0	0.04174648259462316
32-33	0.0	0.0	0.04848404782350221
34-35	0.0	0.0	0.05502344936917894
36-37	0.0	0.0	0.06341237862474404
38-39	0.0	0.0	0.07616090891076029
40-41	0.0	0.0	0.09214611268908118
42-43	0.0	0.0	0.10846158927273929
44-45	0.0	0.0	0.20391043001519255
46-47	0.0	0.0	0.29414096043331794
48-49	0.0	0.0	0.422617081709492
50-51	0.0	0.0	0.9709359931303256
52-53	0.0	0.0	1.0237796419842788
54-55	0.0	0.0	1.0254970605720324
56-57	0.0	0.0	1.0342823171940023
58-59	0.0	0.0	1.0344144263161372
60-61	0.0	0.0	1.0373208270031045
62-63	0.0	0.0	1.0507959574608627
64-65	0.0	0.0	1.0519849395600764
66-67	0.0	0.0	1.0569390316401348
68-69	0.0	0.0	1.0577977409340116
70-71	0.0	0.0	1.0581280137393487
72-73	0.0	0.0	1.0583922319836185
74-75	0.0	0.0	1.059250941277495
76-77	0.0	0.0	1.0652619063346325
78-79	0.0	0.0	1.0659885065063743
80-81	0.0	0.0	1.066252724750644
82-83	0.0	0.0	1.0667811612391835
84-85	0.0	0.0	1.0667811612391835
86-87	0.0	0.0	1.0667811612391835
88-89	0.0	0.0	1.0689609617544091
90-91	0.0	0.0	1.0710086531474998
92-93	0.0	0.0	1.0710086531474998
94-95	0.0	0.0	1.0712728713917696
96-97	0.0	0.0	1.0715370896360394
98-99	0.0	0.0	1.0716031441971068
100-101	0.0	0.0	1.0716691987581743
102-103	0.0	0.0	1.0727260717352531
104-105	0.0	0.0	1.1039698791201533
106-107	0.0	0.0	1.1040359336812207
108-109	0.0	0.0	1.1040359336812207
110-111	0.0	0.0	1.1040359336812207
112-113	0.0	0.0	1.1042340973644231
114-115	0.0	0.0	1.106479952440716
116-117	0.0	0.0	1.1085936983948743
118-119	0.0	0.0	1.1097166259330207
120-121	0.0	0.0	1.1098487350551556
122-123	0.0	0.0	1.1404980513904486
124-125	0.0	0.0	1.1689675672105158
126-127	0.0	0.0	1.1690996763326509
128-129	0.0	0.0	1.1691657308937182
130-131	0.0	0.0	1.169429949137988
132-133	0.0	0.0	1.1696941673822578
134-135	0.0	0.0	1.1696941673822578
136-137	0.0	0.0	1.1696941673822578
138-139	0.0	0.0	1.1710152586036067
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCTTGG	40	0.0	148.15918	1
CGGGTGT	30	5.6024874E-10	148.15918	1
TCTCGCA	10	0.0066479924	148.15918	1
AGTGTGG	20	1.9135423E-6	148.15918	1
TGCCAAG	10	0.0066479924	148.15918	1
CGTGTGG	80	0.0	148.15918	1
GCCCGCA	20	1.9135423E-6	148.15918	1
CGTTGGG	15	1.1286852E-4	148.15918	1
AGTTTGA	70	0.0	148.15918	1
AGTTTCG	50	0.0	148.15918	1
TGCCGAG	45	0.0	148.15916	1
CGTGTGT	75	0.0	148.15916	1
AGTTTTG	75	0.0	148.15916	1
AGTTTGT	230	0.0	148.15916	1
TCCCGCA	10205	0.0	148.0866	1
AGTTTGG	15430	0.0	148.06316	1
TTCCAGA	23495	0.0	148.00151	1
CGTTTGG	450	0.0	146.51297	1
TTAGAAA	190	0.0	144.97855	4
GTTTAGA	190	0.0	144.97855	2
>>END_MODULE