FastQCFastQC Report
Wed 17 Jun 2015
000000000-AG1W8 l01n01 mis16.3410000000d262.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG1W8 l01n01 mis16.3410000000d262.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756950
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC20512627.09901578704009No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG18864624.921857454257214No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA13069317.265737499174318No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG8259410.911420833608561No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC56020.7400753021996169No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC54270.7169562058260124No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG50140.6623951383843054No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG39460.52130259594425No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG36910.4876147697998547No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA30530.40332914987779905No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC29000.3831164541911619No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCCTCCGCGGCTATATTGA28280.3736045973974503No Hit
TCCCGCAGATGATGAAGATGAAGCAGCGTTCGCAATTTTTCCGTCGGCAC25820.341105753352269No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG23960.3165334566351807No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG23900.31574080190237136No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG22320.29486756060505975No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG20600.2721447915978598No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC18540.24493031243807387No Hit
AGTTTAGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA16490.21784794240042277No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA16360.21613052381266926No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC15120.19974899266794371No Hit
AGTTTGTAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA13660.18046106083625074No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG11810.15602087324129732No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA11500.15192549045511594No Hit
TTCCATATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC10970.1449237069819671No Hit
TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG9700.12814584847083693No Hit
TTCCAGATCTCGATGCCGACAGTCCCGGAGCCGCTCAGCGAGCCGTCTTC8860.11704868221150672No Hit
TTCCAGATCTCGATGCCCACAGGCAGAGAATTTTCCGGCACATATTCGCC8690.11480282713521368No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA8350.11031111698262765No Hit
TTCCAGATCTCGATTCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC8170.10793315278419975No Hit
TTCCACATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC8010.10581940683004161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTGG400.0148.159181
CGGGTGT305.6024874E-10148.159181
TCTCGCA100.0066479924148.159181
AGTGTGG201.9135423E-6148.159181
TGCCAAG100.0066479924148.159181
CGTGTGG800.0148.159181
GCCCGCA201.9135423E-6148.159181
CGTTGGG151.1286852E-4148.159181
AGTTTGA700.0148.159181
AGTTTCG500.0148.159181
TGCCGAG450.0148.159161
CGTGTGT750.0148.159161
AGTTTTG750.0148.159161
AGTTTGT2300.0148.159161
TCCCGCA102050.0148.08661
AGTTTGG154300.0148.063161
TTCCAGA234950.0148.001511
CGTTTGG4500.0146.512971
TTAGAAA1900.0144.978554
GTTTAGA1900.0144.978552
Produced by FastQC (version 0.11.2)