##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AG1W8 l01n01 mis15.3410000000d256.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	645230
Sequences flagged as poor quality	0
Sequence length	151
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09488554468949	34.0	34.0	34.0	31.0	34.0
2	33.429139996590365	34.0	34.0	34.0	33.0	34.0
3	33.42831083489608	34.0	34.0	34.0	33.0	34.0
4	33.434790694791005	34.0	34.0	34.0	34.0	34.0
5	33.66647707019202	34.0	34.0	34.0	34.0	34.0
6	36.34631991692885	38.0	38.0	38.0	34.0	38.0
7	36.67097314136044	38.0	38.0	38.0	34.0	38.0
8	36.9222618291152	38.0	37.0	38.0	35.0	38.0
9	37.37575748182819	38.0	38.0	38.0	36.0	38.0
10-14	37.17628504564264	38.0	38.0	38.0	36.2	38.0
15-19	37.27625032933993	38.0	38.0	38.0	36.2	38.0
20-24	37.49608263719914	38.0	38.0	38.0	37.0	38.0
25-29	37.18483114548301	38.0	38.0	38.0	36.2	38.0
30-34	36.79461711327743	38.0	37.8	38.0	35.2	38.0
35-39	37.12434697704695	38.0	38.0	38.0	36.0	38.0
40-44	36.83317762658277	38.0	38.0	38.0	35.2	38.0
45-49	37.04190195744153	38.0	38.0	38.0	36.0	38.0
50-54	37.03407498101452	38.0	37.8	38.0	36.0	38.0
55-59	36.880524464144564	38.0	37.8	38.0	35.6	38.0
60-64	36.61207631387257	38.0	37.0	38.0	34.6	38.0
65-69	36.8037704384483	38.0	37.4	38.0	35.0	38.0
70-74	36.85848519132712	38.0	37.8	38.0	35.4	38.0
75-79	36.49546053345318	38.0	37.4	38.0	34.2	38.0
80-84	36.63949692357764	38.0	37.2	38.0	34.2	38.0
85-89	36.39376346419106	38.0	37.0	38.0	33.2	38.0
90-94	36.660581188103464	38.0	37.0	38.0	34.8	38.0
95-99	36.46707778621577	38.0	37.0	38.0	33.8	38.0
100-104	36.01176200734621	38.0	36.8	38.0	31.8	38.0
105-109	36.1767205492615	38.0	37.0	38.0	33.0	38.0
110-114	36.338697518714255	38.0	37.0	38.0	34.0	38.0
115-119	35.8204237248733	38.0	36.8	38.0	30.8	38.0
120-124	35.94285882553508	38.0	36.6	38.0	31.8	38.0
125-129	35.854063512235946	38.0	37.0	38.0	31.6	38.0
130-134	35.36228414673837	38.0	36.6	38.0	28.6	38.0
135-139	35.23453218232258	38.0	36.2	38.0	28.0	38.0
140-144	35.301119910729504	38.0	36.4	38.0	28.6	38.0
145-149	34.98606140446042	37.8	35.6	38.0	27.2	38.0
150-151	33.545185437750874	36.5	33.0	37.5	24.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.5351264918089669
1101	2	0.3243733599939773
1101	3	0.15703397155290588
1101	4	0.1619043040360566
1101	5	0.1346062775272614
1101	6	1.1403331404625945
1101	7	-0.11102761891201851
1101	8	0.2598173246851303
1101	9	0.14204987675179126
1101	10-14	0.29711231561057616
1101	15-19	0.2432825884530203
1101	20-24	0.15843490079682
1101	25-29	0.2658008928870643
1101	30-34	0.2920129515023575
1101	35-39	0.08651022480040638
1101	40-44	0.1997302678649504
1101	45-49	0.31180113900650497
1101	50-54	0.2684719993235163
1101	55-59	0.36494563117371115
1101	60-64	0.5136025238043018
1101	65-69	0.2783112304032329
1101	70-74	0.1955589173952461
1101	75-79	0.34957178266441247
1101	80-84	0.34848320516222486
1101	85-89	0.3699301239647781
1101	90-94	0.325529269623523
1101	95-99	0.4496296155765549
1101	100-104	0.726544490569502
1101	105-109	0.505091475500123
1101	110-114	0.4462515942360952
1101	115-119	0.6914430235604883
1101	120-124	0.4130803867592192
1101	125-129	0.2740485775121613
1101	130-134	0.449757246319173
1101	135-139	0.4624485686857014
1101	140-144	0.40150255531683143
1101	145-149	0.5548764229101479
1101	150-151	0.44373095252728234
1102	1	0.41314846983094355
1102	2	0.2216261072467276
1102	3	0.07131968583861692
1102	4	0.1261900183217719
1102	5	0.17911177203275486
1102	6	1.1150584151878675
1102	7	0.2307306228462238
1102	8	0.12739974226754924
1102	9	0.12117075587267578
1102	10-14	0.21183759033585403
1102	15-19	0.18712874229917276
1102	20-24	0.06579753815945821
1102	25-29	0.22799869508487092
1102	30-34	0.07421075370015728
1102	35-39	0.12519154348172634
1102	40-44	0.1973126854473648
1102	45-49	0.1824604796658562
1102	50-54	0.15264782349934336
1102	55-59	0.2834071696352538
1102	60-64	0.41415197435375717
1102	65-69	0.19028925238124828
1102	70-74	0.19369078552711727
1102	75-79	0.13649485958749352
1102	80-84	0.32892276560178146
1102	85-89	0.1735564975911572
1102	90-94	0.34069410478836204
1102	95-99	0.3294098353567776
1102	100-104	0.6522587762837802
1102	105-109	0.2299266403352931
1102	110-114	0.16218566017015945
1102	115-119	0.6428715949890673
1102	120-124	0.4408825845614288
1102	125-129	0.31382879729238056
1102	130-134	0.3250319715938943
1102	135-139	0.37101999725712886
1102	140-144	0.301722335536617
1102	145-149	0.398832466866196
1102	150-151	0.7610386448349757
1103	1	0.801483444367129
1103	2	0.29398049832019524
1103	3	0.16394605342375712
1103	4	0.25569300571370945
1103	5	0.18232359594276204
1103	6	1.1482752533249894
1103	7	0.4533151850679502
1103	8	0.20988438242123664
1103	9	0.0406262591379587
1103	10-14	0.1047326117520413
1103	15-19	0.1628640515199109
1103	20-24	0.02376635329154908
1103	25-29	0.14565264091913122
1103	30-34	0.31414840812929157
1103	35-39	0.07717089453199577
1103	40-44	0.29727323616658197
1103	45-49	0.1312319940228477
1103	50-54	0.12943871814619712
1103	55-59	0.28536598047806194
1103	60-64	0.43264057755923346
1103	65-69	0.12080052230977856
1103	70-74	0.11708807542797928
1103	75-79	0.1646571755562931
1103	80-84	0.2815151814322405
1103	85-89	0.11262191815189482
1103	90-94	0.22613067481536575
1103	95-99	0.32324408796507953
1103	100-104	0.5745415786930295
1103	105-109	0.20177846086220796
1103	110-114	0.3007798037945477
1103	115-119	0.6301611937904781
1103	120-124	0.5222285118611723
1103	125-129	0.2268346754164554
1103	130-134	0.3526723246438124
1103	135-139	0.38805652862021844
1103	140-144	0.5356883475345171
1103	145-149	0.4851328130290966
1103	150-151	0.42255520688754444
2116	1	0.608752865435342
2116	2	0.3141169497375671
2116	3	0.052488517007446944
2116	4	-0.45314731101555594
2116	5	0.051256294177278505
2116	6	1.0776624777919324
2116	7	-0.13776754565194693
2116	8	0.08958755445536326
2116	9	0.14209982680174704
2116	10-14	0.24325284175111506
2116	15-19	0.2998926450630819
2116	20-24	0.10257409493601699
2116	25-29	0.2817882388744195
2116	30-34	0.263864433353838
2116	35-39	0.07300373129390891
2116	40-44	0.12618048431517082
2116	45-49	0.28817143537680323
2116	50-54	0.21779600864752524
2116	55-59	0.28827896450704316
2116	60-64	0.3612382214399972
2116	65-69	0.3052076672996691
2116	70-74	0.1917127635490914
2116	75-79	0.11600201909465113
2116	80-84	0.4151831718621892
2116	85-89	0.4066800407146971
2116	90-94	0.41222590632015965
2116	95-99	0.39746844341538434
2116	100-104	0.6946663686913723
2116	105-109	0.4719079923166376
2116	110-114	0.37948502746952784
2116	115-119	0.7473571094745779
2116	120-124	0.6210091246879657
2116	125-129	0.40998264344622726
2116	130-134	0.47112254768448025
2116	135-139	0.5298211960583288
2116	140-144	0.6436537374680213
2116	145-149	0.7406473186810416
2116	150-151	1.131409940206268
1117	1	0.28419221208091017
1117	2	0.29548124044038104
1117	3	0.21263964992122908
1117	4	0.2600891930769791
1117	5	0.19394693686791697
1117	6	1.2834019393679696
1117	7	0.4133363439733486
1117	8	0.32352579520294
1117	9	0.14078207840228174
1117	10-14	0.2754823502452126
1117	15-19	0.24511266499127515
1117	20-24	0.1626573320620608
1117	25-29	0.2758380930410027
1117	30-34	0.40015764727350955
1117	35-39	0.19926560171921182
1117	40-44	0.25711984327008963
1117	45-49	0.38675346294716206
1117	50-54	0.3045296381827143
1117	55-59	0.42816793416254484
1117	60-64	0.5884966957801936
1117	65-69	0.4207506198075919
1117	70-74	0.38927337414472163
1117	75-79	0.33258841583668897
1117	80-84	0.463373956434296
1117	85-89	0.4447960751342279
1117	90-94	0.33001680543402756
1117	95-99	-0.11023740456283093
1117	100-104	0.5620312994804308
1117	105-109	0.5285190207175532
1117	110-114	0.5308113924145701
1117	115-119	0.948638901612405
1117	120-124	0.6743084194269429
1117	125-129	0.5168924651420497
1117	130-134	0.5091530645087659
1117	135-139	0.3678951843268763
1117	140-144	0.4669628537732464
1117	145-149	-0.3760497326463579
1117	150-151	0.1711393418033751
2117	1	-29.968170211487738
2117	2	-10.430056808535973
2117	3	-2.0725866970048585
2117	4	-0.8222458552767904
2117	5	-0.08402021528578274
2117	6	1.246775179984894
2117	7	-0.20011806570311563
2117	8	0.1543613562139754
2117	9	0.2353702584669719
2117	10-14	0.49725422720585755
2117	15-19	0.40948919457285626
2117	20-24	0.26354522792450297
2117	25-29	0.3703519167005638
2117	30-34	0.4756289161595433
2117	35-39	0.2818380278722472
2117	40-44	0.10645342901327837
2117	45-49	0.3811033407815927
2117	50-54	0.2867132808185957
2117	55-59	0.4331285112004082
2117	60-64	0.44744637337483795
2117	65-69	0.4322270687225327
2117	70-74	0.4690891471076881
2117	75-79	0.4262341586109599
2117	80-84	0.5155960272641948
2117	85-89	0.370461775971485
2117	90-94	0.4057597766436203
2117	95-99	0.05003810802408992
2117	100-104	0.7938108251026463
2117	105-109	0.5888042197334826
2117	110-114	0.5979778145089583
2117	115-119	1.0434962920562825
2117	120-124	0.7682301249458376
2117	125-129	0.5050880441915382
2117	130-134	0.6454944158741256
2117	135-139	0.49480155192805597
2117	140-144	0.7800193963933353
2117	145-149	0.9219012057917695
2117	150-151	1.2118699718593646
1116	1	-0.2892595085111722
1116	2	-0.28081492860152935
1116	3	-0.8549283220116735
1116	4	-1.367546454198738
1116	5	-0.3421066232515031
1116	6	1.0242188241266206
1116	7	-0.9108793820950041
1116	8	0.030207890420854255
1116	9	0.08316193125840243
1116	10-14	0.22908052472889295
1116	15-19	0.22833781188696634
1116	20-24	0.021472781219124215
1116	25-29	-0.026960696378637294
1116	30-34	0.12730069099529118
1116	35-39	0.08729156505609836
1116	40-44	-0.07982077640427576
1116	45-49	0.19126839119700634
1116	50-54	-0.028976239581346874
1116	55-59	0.19911428709030332
1116	60-64	0.2230280887396887
1116	65-69	0.17432978436352187
1116	70-74	0.16327948055595698
1116	75-79	0.04692418959884037
1116	80-84	0.12531963662183188
1116	85-89	0.20836333736315993
1116	90-94	0.2980076778524108
1116	95-99	-0.43064980734377656
1116	100-104	0.5509368240118562
1116	105-109	0.32557529820054043
1116	110-114	0.21649983870852907
1116	115-119	0.6523883986413921
1116	120-124	0.4152627389225785
1116	125-129	0.12573750289963215
1116	130-134	0.35248626524197846
1116	135-139	0.39043581892754275
1116	140-144	0.20177703700994698
1116	145-149	0.26131470280630964
1116	150-151	0.11789042193690591
2118	1	0.6850057300524526
2118	2	0.35154074011245484
2118	3	0.14101157349003302
2118	4	0.1133756825732064
2118	5	0.1119586609504708
2118	6	1.2433768926631785
2118	7	0.5635772029103379
2118	8	0.16203829090272848
2118	9	0.050384908779911086
2118	10-14	0.2285985808911164
2118	15-19	0.16743432204825837
2118	20-24	0.03508155375039479
2118	25-29	0.1332902219952885
2118	30-34	0.2959703544142229
2118	35-39	0.03652826185048497
2118	40-44	-0.02733154609400401
2118	45-49	0.16741313240939348
2118	50-54	0.055901410833875786
2118	55-59	0.22241006668198793
2118	60-64	0.21058903925625572
2118	65-69	0.21097503090851433
2118	70-74	0.24441955647510838
2118	75-79	0.2242505440379432
2118	80-84	0.1109293874229067
2118	85-89	0.1920837600981855
2118	90-94	0.19216906602724038
2118	95-99	0.3172483543420057
2118	100-104	0.6537928521011622
2118	105-109	0.2309029883268181
2118	110-114	0.2959757879737879
2118	115-119	0.6863066646973195
2118	120-124	0.42396179315496596
2118	125-129	0.28182507869507134
2118	130-134	0.4131656928473504
2118	135-139	0.3965216681550956
2118	140-144	0.33148692732481777
2118	145-149	0.2743768831020077
2118	150-151	0.8823110907701519
1119	1	1.1185644823898144
1119	2	0.2537591527726306
1119	3	0.049518272965777044
1119	4	-0.06538180271433447
1119	5	0.16779897768424945
1119	6	1.288655353963378
1119	7	0.30094255377243684
1119	8	-0.058206615660239436
1119	9	0.06018174488366412
1119	10-14	0.08732424653680937
1119	15-19	0.1369737187512925
1119	20-24	-0.11072718711526619
1119	25-29	0.10577734500638059
1119	30-34	0.08986224506593743
1119	35-39	0.08227749324624511
1119	40-44	0.0748107231239743
1119	45-49	0.04105545611796657
1119	50-54	0.015546175147697738
1119	55-59	0.24915677717056894
1119	60-64	0.2983062129722782
1119	65-69	0.187865782993498
1119	70-74	0.2409082109588212
1119	75-79	0.2938084388653621
1119	80-84	0.2179102067320855
1119	85-89	0.2529697628972727
1119	90-94	0.22761914403482564
1119	95-99	0.29209821840230177
1119	100-104	0.6030044591723183
1119	105-109	0.26003653044518416
1119	110-114	0.30459146864740205
1119	115-119	0.7398770894945628
1119	120-124	0.5577173569319029
1119	125-129	0.18521812225313283
1119	130-134	0.5141940595059751
1119	135-139	0.36154393760249803
1119	140-144	0.5489240623812037
1119	145-149	0.41569667408754896
1119	150-151	0.7130500263106399
2119	1	0.5573399925938958
2119	2	0.2518458874665086
2119	3	0.14291396457575445
2119	4	0.18724007937183273
2119	5	0.07716689151644829
2119	6	1.2911355126935362
2119	7	0.4480513973098539
2119	8	0.34350823694747135
2119	9	0.05337902379522319
2119	10-14	0.21436506286332957
2119	15-19	0.16008879811637655
2119	20-24	0.03718947955140095
2119	25-29	0.10027847879322849
2119	30-34	0.17024773259428372
2119	35-39	0.15275651818956248
2119	40-44	-0.1381853086220559
2119	45-49	0.08873119307941835
2119	50-54	0.11275422503431543
2119	55-59	0.3011692488259001
2119	60-64	0.0958543989133247
2119	65-69	0.12464299387784905
2119	70-74	0.18889573930349712
2119	75-79	-0.048467638232146726
2119	80-84	0.14597143122188072
2119	85-89	0.3089079715140599
2119	90-94	0.2264711848511567
2119	95-99	0.24059595082860596
2119	100-104	0.5868479965872808
2119	105-109	0.2700138547082247
2119	110-114	0.3289395412097562
2119	115-119	0.6796446631907074
2119	120-124	0.18488572427884264
2119	125-129	0.23652197712841883
2119	130-134	0.13509825451732382
2119	135-139	0.035197565720416435
2119	140-144	0.07344220297076731
2119	145-149	0.0653281933619212
2119	150-151	0.0324236090770782
1118	1	0.17745548064958427
1118	2	0.3177373603347249
1118	3	0.1171796398381062
1118	4	0.18760921927585628
1118	5	0.21000452269294811
1118	6	1.3730318371147803
1118	7	0.47288923942344496
1118	8	0.5402202550880588
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2111	2	0.457649978602376
2111	3	0.15496985406387154
2111	4	0.22847481152624027
2111	5	0.11503458030713176
2111	6	1.0172619741434659
2111	7	0.6509253291876149
2111	8	0.09598831318440659
2111	9	-0.055816924665649026
2111	10-14	-0.05186643555676085
2111	15-19	-0.10007859053096979
2111	20-24	-0.06271350841910106
2111	25-29	-0.005949033554358607
2111	30-34	-0.04723713484901282
2111	35-39	-0.08019563046190825
2111	40-44	-0.06678471889451032
2111	45-49	-0.13443752337883552
2111	50-54	-0.3510605519436112
2111	55-59	-0.11552539954178798
2111	60-64	-0.08285707015237165
2111	65-69	-0.21832541583761156
2111	70-74	-0.16109013785683146
2111	75-79	-0.23150294005174743
2111	80-84	-0.10385275922264015
2111	85-89	-0.16234571428311995
2111	90-94	-0.5245385321904905
2111	95-99	-0.30864629942811916
2111	100-104	-0.1019283055517306
2111	105-109	-0.1249958992903899
2111	110-114	-0.357674514579422
2111	115-119	-1.714091395454723
2111	120-124	-0.6747398488840588
2111	125-129	-0.29892753833938457
2111	130-134	0.024063301149091387
2111	135-139	0.10683138028620931
2111	140-144	-0.5566094839826263
2111	145-149	-0.6870850963621962
2111	150-151	-1.593175621690122
2110	1	1.4581269583235823
2110	2	0.4415359144206832
2110	3	0.2124947843250382
2110	4	0.1540059523877062
2110	5	0.16286540047978093
2110	6	1.3009411645140148
2110	7	0.4337279792633879
2110	8	-0.06251447087779383
2110	9	-0.03112553274436891
2110	10-14	-0.054536554387340175
2110	15-19	-0.06006854154905028
2110	20-24	-0.05789699843602136
2110	25-29	-0.13287783404637565
2110	30-34	0.04731074333316343
2110	35-39	-0.11274801695783054
2110	40-44	-0.10026869543590067
2110	45-49	-0.09884394703952637
2110	50-54	-0.2189046334776492
2110	55-59	-0.2820282440077193
2110	60-64	-0.01307952056641426
2110	65-69	-0.0625409362979994
2110	70-74	-0.06562577055831298
2110	75-79	-0.11705505747657696
2110	80-84	-0.15095049792714121
2110	85-89	-0.10849538920130186
2110	90-94	-0.11205884980421388
2110	95-99	-0.3914164138657057
2110	100-104	0.41418081650770944
2110	105-109	-0.03020942642813651
2110	110-114	-0.3475191397468933
2110	115-119	-6.372875605923234
2110	120-124	-2.563326154812408
2110	125-129	0.05554686695856503
2110	130-134	-0.21715701866113335
2110	135-139	-0.3184305521934192
2110	140-144	-0.2422412244882608
2110	145-149	-0.20060097704838142
2110	150-151	-0.5387669740744201
2109	1	1.1717498342004404
2109	2	0.4096740672821184
2109	3	0.0037171855405162546
2109	4	0.06682362800164299
2109	5	0.12092743780615933
2109	6	1.1830644525542695
2109	7	0.7663165674555827
2109	8	0.1472339343461755
2109	9	-0.03143936958154114
2109	10-14	0.09998552464028165
2109	15-19	0.04750246908638189
2109	20-24	-0.18273246981086544
2109	25-29	-0.08013129029240673
2109	30-34	0.0034026126692836556
2109	35-39	-0.23364321969912538
2109	40-44	0.02126967078071118
2109	45-49	-0.2579384132744451
2109	50-54	-0.09623739750467308
2109	55-59	-0.2880078459602373
2109	60-64	-0.11393307594716617
2109	65-69	-0.2075405591550208
2109	70-74	-0.2520446497971278
2109	75-79	-0.12993550045794677
2109	80-84	-0.17031955119050224
2109	85-89	-0.29198319401364614
2109	90-94	-0.42765007530339716
2109	95-99	-0.06331953122181488
2109	100-104	-0.07279158740164604
2109	105-109	-0.07121907859455234
2109	110-114	-0.8366209872087325
2109	115-119	-4.788936037698065
2109	120-124	-1.1749890994370489
2109	125-129	-0.4842975480926768
2109	130-134	-0.5362082616166433
2109	135-139	-0.585078182119183
2109	140-144	-0.3833101419801537
2109	145-149	-0.0630506015217307
2109	150-151	-1.031020053177464
2108	1	0.8597796896328944
2108	2	0.14623330624417008
2108	3	0.14361426666978616
2108	4	0.1673752668240951
2108	5	0.013138951181886682
2108	6	0.9025949063828946
2108	7	0.2551469448235224
2108	8	-0.2918574672335623
2108	9	-0.33279311150582913
2108	10-14	-0.21351421013789462
2108	15-19	-0.36794981790134074
2108	20-24	-0.46299729258659283
2108	25-29	-0.3495914010418062
2108	30-34	-6.2177353226361625
2108	35-39	-0.6975617941496637
2108	40-44	-0.6576924215089619
2108	45-49	-0.10003026623952849
2108	50-54	-0.08958083775370795
2108	55-59	-0.25672974025776085
2108	60-64	-0.45814659062773444
2108	65-69	-0.3207193171639062
2108	70-74	-0.2563552857384721
2108	75-79	-0.4129727854899059
2108	80-84	-0.17511274404348853
2108	85-89	-0.5695680904114724
2108	90-94	-0.52604048560476
2108	95-99	-0.5481741034466694
2108	100-104	-0.3842458199769112
2108	105-109	-0.33188361269447597
2108	110-114	-0.5794541436647549
2108	115-119	-0.5740213472697278
2108	120-124	-0.5619010326124396
2108	125-129	-0.20233417618766936
2108	130-134	-0.46931604031509266
2108	135-139	-1.0296368804729141
2108	140-144	-1.0260263297730248
2108	145-149	-0.9710819101213559
2108	150-151	-1.0286885857459183
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	6.0
14	22.0
15	14.0
16	34.0
17	43.0
18	22.0
19	21.0
20	74.0
21	171.0
22	375.0
23	640.0
24	1091.0
25	1527.0
26	2102.0
27	3082.0
28	4215.0
29	6046.0
30	8389.0
31	12085.0
32	17382.0
33	23139.0
34	30464.0
35	50336.0
36	103887.0
37	380063.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.15552616783205708	21.33464258281135	77.26467164498222	1.2451596043743713
2	54.70266416626629	0.05966864528927669	17.702834648109977	27.534832540334452
3	0.1692419757295848	0.0773367636346729	22.12203400337864	77.6313872572571
4	0.7344667792880059	0.24146428405374828	21.85530740976086	77.16876152689738
5	60.032546533794154	17.77211227004324	21.936053810269208	0.2592873858934024
6	40.22503603366241	32.782573655905644	0.4136509461742324	26.578739364257707
7	21.701408799962802	44.88972924383553	1.0170016893200875	32.39186026688158
8	59.783643042016024	22.527160857368692	17.429598747733365	0.2595973528819181
9	32.45881313640097	49.556747206422514	0.5319033522929808	17.45253630488353
10-14	14.71224214621143	45.978364304201605	22.413774932969638	16.89561861661733
15-19	30.161430807618988	24.573159958464423	26.990468515103146	18.274940718813447
20-24	24.866140756009486	28.161275824124733	16.930799869813864	30.04178355005192
25-29	26.820234645010306	34.397749639663374	15.33781752243386	23.444198192892458
30-34	22.00232475241387	14.873424980239605	29.948328503014427	33.1759217643321
35-39	34.09373401732715	19.595927033770906	23.564403391038855	22.74593555786309
40-44	13.957782496164159	26.87404491421664	28.07256327201153	31.095609317607675
45-49	8.259070409001442	24.342265548719062	36.14959006865769	31.24907397362181
50-54	22.87550175906266	34.797421074655546	12.125505633650016	30.201571532631778
55-59	14.997101808657378	33.325139872603565	47.30483703485579	4.372921283883267
60-64	26.22934457480278	28.74172000681927	25.563938440556083	19.464996977821862
65-69	17.493637927560716	42.7304372084373	19.204438727275544	20.57148613672644
70-74	16.10904638655983	16.880988174759388	34.92971498535406	32.08025045332672
75-79	27.779179517381397	29.647412550563363	21.594377198828322	20.97903073322691
80-84	5.419245850316941	31.084388512623406	31.771151372378842	31.72521426468081
85-89	23.363079831997894	32.09909644622847	30.73068518202811	13.807138539745518
90-94	6.999612541264355	50.7057328394526	28.732111030175282	13.56254358910776
95-99	11.527517319405483	42.047889899725675	38.92295770500442	7.50163507586442
100-104	17.71278458844133	42.306278381352385	16.470157928180647	23.510779102025634
105-109	19.029028408474495	42.04079165568867	27.338251476217785	11.591928459619051
110-114	32.77770717418595	11.032097081660803	29.568649938781522	26.621545805371728
115-119	16.558281543015667	46.89028718441486	11.380159013065109	25.171272259504363
120-124	4.547246718224509	21.691148892642932	52.93079986981386	20.830804519318693
125-129	18.42285696573315	34.35407529098151	10.211211505974614	37.011856237310724
130-134	9.895417138074796	60.08573686902346	25.98230088495575	4.0365451079460035
135-139	31.613316181826633	13.857508175379321	41.46124637726082	13.067929265533222
140-144	17.901895448134773	34.638470002944686	28.39663375850472	19.06300079041582
145-149	29.71160671388497	31.003611115416206	28.64687010833346	10.637912062365357
150-151	34.26987275855121	39.24771011887234	9.329851370829006	17.15256575174744
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	1.5
26	2.0
27	1.5
28	2.5
29	2.5
30	1.5
31	6.0
32	28.5
33	105.5
34	451.5
35	4247.0
36	92966.0
37	99672.0
38	11466.0
39	1137.5
40	983.5
41	5282.5
42	52981.5
43	59063.5
44	13764.5
45	4662.0
46	8984.5
47	79012.0
48	82945.5
49	18416.5
50	50995.0
51	49697.5
52	6509.5
53	953.0
54	242.0
55	173.0
56	142.5
57	51.0
58	41.0
59	38.0
60	50.0
61	43.5
62	14.0
63	18.5
64	13.5
65	0.5
66	1.0
67	1.5
68	3.5
69	6.5
70	21.0
71	22.0
72	4.5
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.142646808114936
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	645230.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	1.7324758987621882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.77945009012711	1.260886432063108
2	9.457729763063808	0.32770577743027735
3	3.8336189711748325	0.19924957417793687
4	2.0662368651273373	0.143188222798682
5	1.4098505435305086	0.1221266043761689
6	0.8819588190621668	0.09167834386355794
7	0.6290033847156624	0.07628132429818081
8	0.5478233180334233	0.07592725562182714
9	0.5566893280611219	0.08680057595676098
>10	4.569723178080175	1.74638630669945
>50	1.427468688199987	1.7852658126471577
>100	1.4992910121345777	5.301670194275224
>500	0.11671127639370964	1.4110412486787782
>1k	0.1885336003283002	8.189856365650469
>5k	0.0	0.0
>10k+	0.03591116196729527	79.18193596146243
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	178375	27.645180788246055	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	139471	21.615702927638207	No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	105750	16.389504517768856	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	85488	13.249228957116067	No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	4778	0.7405111355640625	No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG	4103	0.6358972769400059	No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG	4076	0.6317127225950436	No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	3964	0.6143545712381632	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG	3713	0.5754537141794399	No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	3608	0.5591804472823645	No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	3424	0.5306634843389179	No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	2456	0.3806394618973079	No Hit
AGTTTGGAAACTCCGAGCCCACGAGACATACTGTTTGTGATCAATTTGCC	2374	0.3679308153681633	No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC	2253	0.34917781256296204	No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCCTCCGCGGCTATATTGA	2249	0.3485578785859306	No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	2218	0.3437533902639369	No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	1934	0.29973807789470425	No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC	1785	0.27664553725028285	No Hit
TTCCAGATCTCGATGCCGACGACGGGAAAGCAATAGAATCCAAAGTACCT	1772	0.2746307518249307	No Hit
AGTTTGGAAACTCCGAGCCCACGAGACGTTTGTGATCAATTTGCCACTGT	1693	0.2623870557785596	No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC	1436	0.22255629775428917	No Hit
AGTTTAGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	1349	0.20907273375385524	No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA	1261	0.1954341862591634	No Hit
TTCCAGATCTCGATGCCGACGAGGGAAAGCAATAGAATCCAAAGTACCTG	1136	0.1760612494769307	No Hit
AGTTTGTAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	1073	0.16629728933868543	No Hit
TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	996	0.1543635602808301	No Hit
TCCCGCAGATCATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	831	0.12879128372828294	No Hit
TTCCAGATCTCGGGAAAGCAATAGAATCCAAAGTACCTGTCTCTTATACA	800	0.12398679540628922	No Hit
TCCCGTAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	788	0.12212699347519489	No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	774	0.11995722455558483	No Hit
TCCCGCAGATGATGAAGCTGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	742	0.11499775273933326	No Hit
TTCCAGATCTCGATGCCGACGAGACGGGAAAGCAATAGAATCCAAAGTAC	681	0.10554375958960371	No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA	670	0.10383894115276722	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10-11	0.0	0.0	0.0
12-13	0.0	0.0	1.5498349425786153E-4
14-15	0.0	0.0	3.0996698851572306E-4
16-17	0.0	0.0	3.0996698851572306E-4
18-19	0.0	0.0	0.0016273266897075462
20-21	0.0	0.0	0.03533623669079243
22-23	0.0	0.0	0.04153557646110689
24-25	0.0	0.0	0.042233002185267265
26-27	0.0	0.0	0.06811524572633015
28-29	0.0	0.0	0.070517489887327
30-31	0.0	0.0	0.07198983308277668
32-33	0.0	0.0	0.07927405731289618
34-35	0.0	0.0	0.08772065774994964
36-37	0.0	0.0	0.1745114145343521
38-39	0.0	0.0	0.25525781504269796
40-41	0.0	0.0	0.2870294313655596
42-43	0.0	0.0	0.35088263099979855
44-45	0.0	0.0	0.44720487268105946
46-47	0.0	0.0	0.7932055236117354
48-49	0.0	0.0	1.434914681586411
50-51	0.0	0.0	1.8208235822884862
52-53	0.0	0.0	2.3485423802365046
54-55	0.0	0.0	2.349239805960665
56-57	0.0	0.0	2.349239805960665
58-59	0.0	0.0	2.419137361870961
60-61	0.0	0.0	2.4966291089998913
62-63	0.0	0.0	2.5066255443795233
64-65	0.0	0.0	2.5067030361266527
66-67	0.0	0.0	2.507090494862297
68-69	0.0	0.0	2.523906203989275
70-71	0.0	0.0	2.524216170977791
72-73	0.0	0.0	2.5245261379663066
74-75	0.0	0.0	2.5322753126791997
76-77	0.0	0.0	2.538862111185159
78-79	0.0	0.0	2.548703563070533
80-81	0.0	0.0	2.548703563070533
82-83	0.0	0.0	2.548703563070533
84-85	0.0	0.0	2.548703563070533
86-87	0.0	0.0	2.548703563070533
88-89	0.0	0.0	2.548703563070533
90-91	0.0	0.0	2.5490135300590486
92-93	0.0	0.0	2.5490910218061775
94-95	0.0	0.0	2.5491685135533064
96-97	0.0	0.0	2.5491685135533064
98-99	0.0	0.0	2.5492460053004353
100-101	0.0	0.0	2.5493234970475642
102-103	0.0	0.0	2.5494009887946936
104-105	0.0	0.0	2.5494784805418225
106-107	0.0	0.0	2.5496334640360803
108-109	0.0	0.0	2.549788447530338
110-111	0.0	0.0	2.5504083815073697
112-113	0.0	0.0	2.5604048168870017
114-115	0.0	0.0	2.5604823086341306
116-117	0.0	0.0	2.5604823086341306
118-119	0.0	0.0	2.5606372921283884
120-121	0.0	0.0	2.5718735954620833
122-123	0.0	0.0	2.5815600638531997
124-125	0.0	0.0	2.5877594036235143
126-127	0.0	0.0	2.587836895370643
128-129	0.0	0.0	2.587914387117772
130-131	0.0	0.0	2.5883018458534166
132-133	0.0	0.0	2.5888442880833193
134-135	0.0	0.0	2.590084156037382
136-137	0.0	0.0	2.591479007485703
138-139	0.0	0.0	2.5916339909799606
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCCACA	55	0.0	148.15895	1
CGTTAGG	10	0.0066477926	148.15895	1
CGCCTGT	10	0.0066477926	148.15895	1
TTCCGCA	40	0.0	148.15895	1
TGCCGCG	10	0.0066477926	148.15895	1
TTTCAGA	20	1.9133367E-6	148.15895	1
GGTTTGG	10	0.0066477926	148.15895	1
AGTATGG	10	0.0066477926	148.15895	1
CGGGTGT	35	9.094947E-12	148.15895	1
CCCCGCA	25	3.2619937E-8	148.15895	1
AGTGTGG	20	1.9133367E-6	148.15895	1
TCCCGGA	380	0.0	148.15895	1
AGTCTGG	20	1.9133367E-6	148.15895	1
TCCTGCA	25	3.2619937E-8	148.15895	1
AGTTTTG	35	9.094947E-12	148.15895	1
AGTTTGT	170	0.0	148.15895	1
AGCTTGG	15	1.1286138E-4	148.15894	1
CTCCAGA	15	1.1286138E-4	148.15894	1
TGCTGAC	15	1.1286138E-4	148.15894	1
TCTCGCA	45	0.0	148.15894	1
>>END_MODULE