FastQCFastQC Report
Wed 17 Jun 2015
000000000-AG1W8 l01n01 mis15.3410000000d256.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG1W8 l01n01 mis15.3410000000d256.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645230
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG17837527.645180788246055No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG13947121.615702927638207No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA10575016.389504517768856No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC8548813.249228957116067No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG47780.7405111355640625No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG41030.6358972769400059No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG40760.6317127225950436No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG39640.6143545712381632No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG37130.5754537141794399No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG36080.5591804472823645No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG34240.5306634843389179No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA24560.3806394618973079No Hit
AGTTTGGAAACTCCGAGCCCACGAGACATACTGTTTGTGATCAATTTGCC23740.3679308153681633No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC22530.34917781256296204No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCCTCCGCGGCTATATTGA22490.3485578785859306No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC22180.3437533902639369No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG19340.29973807789470425No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC17850.27664553725028285No Hit
TTCCAGATCTCGATGCCGACGACGGGAAAGCAATAGAATCCAAAGTACCT17720.2746307518249307No Hit
AGTTTGGAAACTCCGAGCCCACGAGACGTTTGTGATCAATTTGCCACTGT16930.2623870557785596No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC14360.22255629775428917No Hit
AGTTTAGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA13490.20907273375385524No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA12610.1954341862591634No Hit
TTCCAGATCTCGATGCCGACGAGGGAAAGCAATAGAATCCAAAGTACCTG11360.1760612494769307No Hit
AGTTTGTAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA10730.16629728933868543No Hit
TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG9960.1543635602808301No Hit
TCCCGCAGATCATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG8310.12879128372828294No Hit
TTCCAGATCTCGGGAAAGCAATAGAATCCAAAGTACCTGTCTCTTATACA8000.12398679540628922No Hit
TCCCGTAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG7880.12212699347519489No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC7740.11995722455558483No Hit
TCCCGCAGATGATGAAGCTGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG7420.11499775273933326No Hit
TTCCAGATCTCGATGCCGACGAGACGGGAAAGCAATAGAATCCAAAGTAC6810.10554375958960371No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA6700.10383894115276722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCACA550.0148.158951
CGTTAGG100.0066477926148.158951
CGCCTGT100.0066477926148.158951
TTCCGCA400.0148.158951
TGCCGCG100.0066477926148.158951
TTTCAGA201.9133367E-6148.158951
GGTTTGG100.0066477926148.158951
AGTATGG100.0066477926148.158951
CGGGTGT359.094947E-12148.158951
CCCCGCA253.2619937E-8148.158951
AGTGTGG201.9133367E-6148.158951
TCCCGGA3800.0148.158951
AGTCTGG201.9133367E-6148.158951
TCCTGCA253.2619937E-8148.158951
AGTTTTG359.094947E-12148.158951
AGTTTGT1700.0148.158951
AGCTTGG151.1286138E-4148.158941
CTCCAGA151.1286138E-4148.158941
TGCTGAC151.1286138E-4148.158941
TCTCGCA450.0148.158941
Produced by FastQC (version 0.11.2)