##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AG1W8 l01n01 mis14.3410000000d249.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	762256
Sequences flagged as poor quality	0
Sequence length	151
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39964263974308	34.0	34.0	34.0	32.0	34.0
2	33.48263313112655	34.0	34.0	34.0	34.0	34.0
3	33.587710690371736	34.0	34.0	34.0	34.0	34.0
4	33.579182059570535	34.0	34.0	34.0	34.0	34.0
5	33.72325963980605	34.0	34.0	34.0	34.0	34.0
6	36.416064944060786	38.0	38.0	38.0	34.0	38.0
7	36.89378109191663	38.0	38.0	38.0	34.0	38.0
8	36.9494802271153	38.0	38.0	38.0	35.0	38.0
9	37.3969165214836	38.0	38.0	38.0	37.0	38.0
10-14	37.23929913309964	38.0	38.0	38.0	36.4	38.0
15-19	37.21415298797255	38.0	38.0	38.0	36.2	38.0
20-24	37.4778402531433	38.0	38.0	38.0	37.0	38.0
25-29	37.20818517663357	38.0	38.0	38.0	36.2	38.0
30-34	36.92375186289121	38.0	38.0	38.0	35.6	38.0
35-39	37.07987946306752	38.0	38.0	38.0	35.8	38.0
40-44	36.995593868726516	38.0	38.0	38.0	35.8	38.0
45-49	37.06116212925841	38.0	38.0	38.0	36.0	38.0
50-54	37.04335839927794	38.0	38.0	38.0	36.0	38.0
55-59	36.721765391154676	38.0	37.8	38.0	35.0	38.0
60-64	36.650553882160324	38.0	37.2	38.0	34.4	38.0
65-69	36.73961634936294	38.0	37.4	38.0	34.8	38.0
70-74	36.82001742196847	38.0	38.0	38.0	35.6	38.0
75-79	36.447394313721375	38.0	37.2	38.0	33.8	38.0
80-84	36.62576352301589	38.0	37.2	38.0	34.2	38.0
85-89	36.35496631053085	38.0	37.0	38.0	33.4	38.0
90-94	36.589895520664975	38.0	37.0	38.0	34.4	38.0
95-99	36.43021294683151	38.0	37.0	38.0	34.0	38.0
100-104	36.05782545496526	38.0	36.8	38.0	32.4	38.0
105-109	36.1183909342793	38.0	37.0	38.0	32.8	38.0
110-114	36.115454650406164	38.0	37.0	38.0	33.0	38.0
115-119	35.77193908608132	38.0	36.8	38.0	30.4	38.0
120-124	35.97222140593187	38.0	36.8	38.0	31.6	38.0
125-129	35.84848659767847	38.0	37.0	38.0	31.4	38.0
130-134	35.3572227703029	38.0	36.4	38.0	28.8	38.0
135-139	35.16811674817909	38.0	36.2	38.0	28.4	38.0
140-144	35.55535017107114	38.0	37.0	38.0	30.2	38.0
145-149	35.178949066980124	37.8	35.6	38.0	28.2	38.0
150-151	33.22784471358704	36.0	32.5	37.5	24.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.43863196220569733
1101	2	0.31535129836317566
1101	3	0.108204747489971
1101	4	0.10653398002156678
1101	5	0.15732281099607093
1101	6	1.1150444251826102
1101	7	0.07428872846343637
1101	8	0.3817327056266109
1101	9	0.15167711856455668
1101	10-14	0.28860351028350095
1101	15-19	0.3166322446715668
1101	20-24	0.18322090407319536
1101	25-29	0.27882528775198523
1101	30-34	0.24793897217206506
1101	35-39	0.15686784797858877
1101	40-44	0.17846759035144544
1101	45-49	0.2650562721411447
1101	50-54	0.2428287873569417
1101	55-59	0.34740688228458794
1101	60-64	0.4785589643771857
1101	65-69	0.2674472508497985
1101	70-74	0.27436922733797076
1101	75-79	0.3060846978009266
1101	80-84	0.4043261748236091
1101	85-89	0.3823961861337679
1101	90-94	0.3321470333286243
1101	95-99	0.48878367841559367
1101	100-104	0.69153968833119
1101	105-109	0.49968879534868904
1101	110-114	0.3079435397811494
1101	115-119	0.6768441121516204
1101	120-124	0.48730232191289247
1101	125-129	0.4360700023165549
1101	130-134	0.4519092988496922
1101	135-139	0.48467394520471174
1101	140-144	0.37456753656173447
1101	145-149	0.5670957754490189
1101	150-151	0.4501767788611417
1102	1	0.6430897645032303
1102	2	0.31462986560587325
1102	3	0.0829244846765036
1102	4	0.12622121423948585
1102	5	0.1557175891453042
1102	6	1.0814887444317804
1102	7	0.4396104113898929
1102	8	0.1424871712183915
1102	9	0.22075577692606885
1102	10-14	0.25074993803651324
1102	15-19	0.2747013319345868
1102	20-24	0.15725104574211457
1102	25-29	0.2674564947683251
1102	30-34	0.19419956835721308
1102	35-39	0.2557297232689919
1102	40-44	0.29071260938586363
1102	45-49	0.29921446019914555
1102	50-54	0.2231046436790649
1102	55-59	0.3168746976883767
1102	60-64	0.5241343579497766
1102	65-69	0.3366751774076704
1102	70-74	0.25740971543048374
1102	75-79	0.2295049502706945
1102	80-84	0.43505726291449776
1102	85-89	0.43929807399604215
1102	90-94	0.4291692777317522
1102	95-99	0.4798760897400456
1102	100-104	0.7936313451628791
1102	105-109	0.37979731368974257
1102	110-114	0.3787397334421527
1102	115-119	0.6886697672605706
1102	120-124	0.5258854334120713
1102	125-129	0.5093177540096292
1102	130-134	0.5869993715183028
1102	135-139	0.7199609739977149
1102	140-144	0.5458192337175731
1102	145-149	0.680217745137071
1102	150-151	1.0653587492919172
1103	1	0.7971148291274872
1103	2	0.30717342931932734
1103	3	0.12614676136781355
1103	4	0.17130126872465468
1103	5	0.19203063284378885
1103	6	1.2004050648378453
1103	7	0.38986013507484074
1103	8	0.33394365453727914
1103	9	0.18353148146161402
1103	10-14	0.2316590552574027
1103	15-19	0.32827289617625866
1103	20-24	0.13678329093983876
1103	25-29	0.24351060729502194
1103	30-34	0.397748973126653
1103	35-39	0.21745000379886648
1103	40-44	0.3726305704973427
1103	45-49	0.2561687357110216
1103	50-54	0.25282203352838906
1103	55-59	0.4660400543835195
1103	60-64	0.521335661831202
1103	65-69	0.33534361924403555
1103	70-74	0.3152853727527898
1103	75-79	0.3434051801865152
1103	80-84	0.38629927872098335
1103	85-89	0.29183411253362834
1103	90-94	0.35276866341690294
1103	95-99	0.4572919696700737
1103	100-104	0.6252018095711591
1103	105-109	0.4059985832013808
1103	110-114	0.4077962283382277
1103	115-119	0.8701215654339052
1103	120-124	0.7104774549167132
1103	125-129	0.5424609078180822
1103	130-134	0.5884769108581551
1103	135-139	0.5619716250158788
1103	140-144	0.6769084305678561
1103	145-149	0.6772746958851528
1103	150-151	0.8352068528933216
2116	1	0.7196806036705397
2116	2	0.3206953734682827
2116	3	0.014259292782973887
2116	4	-0.26415277665140025
2116	5	0.027239147321147072
2116	6	1.0512975775930329
2116	7	0.011928403685082856
2116	8	0.14386080537020973
2116	9	0.03520616282713718
2116	10-14	0.260263451652051
2116	15-19	0.298713740414037
2116	20-24	0.12675079942228962
2116	25-29	0.239354233344649
2116	30-34	0.2660108590542549
2116	35-39	0.14584323801703647
2116	40-44	0.07001976172099234
2116	45-49	0.2784819748603624
2116	50-54	0.19947794751693237
2116	55-59	0.334640397510384
2116	60-64	0.33299384994579384
2116	65-69	0.3276153264075745
2116	70-74	0.2496864090444788
2116	75-79	0.24443325326861753
2116	80-84	0.41562149576506613
2116	85-89	0.32963195533594103
2116	90-94	0.3755328986521249
2116	95-99	0.39213102038355174
2116	100-104	0.6396235044534748
2116	105-109	0.42541999323424307
2116	110-114	0.44750760578618554
2116	115-119	0.8578863003231021
2116	120-124	0.6477597668857129
2116	125-129	0.5483138638550713
2116	130-134	0.6032034413636609
2116	135-139	0.7541013744137217
2116	140-144	0.8164176115929607
2116	145-149	0.836320814414286
2116	150-151	1.461767104158568
1117	1	0.6428222785792812
1117	2	0.34486261025752185
1117	3	0.16157582702749806
1117	4	0.06173979061286872
1117	5	0.13129161302673964
1117	6	1.1730550545113019
1117	7	0.3643215539330029
1117	8	0.23227905167632912
1117	9	0.12889008311223904
1117	10-14	0.22265935919150337
1117	15-19	0.23898553595663685
1117	20-24	0.12599155614353919
1117	25-29	0.1963210056290734
1117	30-34	0.23438980689815025
1117	35-39	0.15208252763333974
1117	40-44	0.2038906884814935
1117	45-49	0.28578192771350786
1117	50-54	0.23843819452508086
1117	55-59	0.4520340135805938
1117	60-64	0.472650289538997
1117	65-69	0.3654885606352565
1117	70-74	0.28405186027571006
1117	75-79	0.23172157468034982
1117	80-84	0.33782079806679377
1117	85-89	0.3901856533532282
1117	90-94	0.2663504591172767
1117	95-99	-0.23027333783096537
1117	100-104	0.33715417579279716
1117	105-109	0.4657826005968744
1117	110-114	0.4767151338507958
1117	115-119	0.8105506626594448
1117	120-124	0.601840134875907
1117	125-129	0.44581978544446343
1117	130-134	0.4618312700577505
1117	135-139	0.44941896640421675
1117	140-144	0.3819821458600714
1117	145-149	-0.5777623579202
1117	150-151	0.20607183851281974
2117	1	-30.28377448023864
2117	2	-10.476923736772356
2117	3	-2.0710362464970906
2117	4	-0.6952059825472787
2117	5	-0.0720289815681241
2117	6	1.1722953583671867
2117	7	-0.1397977939925923
2117	8	0.1676010279473985
2117	9	0.2064369418589891
2117	10-14	0.41147777205869573
2117	15-19	0.41911530515236706
2117	20-24	0.25106502824276333
2117	25-29	0.34010591792819156
2117	30-34	0.41121105987753737
2117	35-39	0.26213213364680144
2117	40-44	0.10083824517791129
2117	45-49	0.3285341386494025
2117	50-54	0.23784460430607623
2117	55-59	0.48539742997817825
2117	60-64	0.42613464775661214
2117	65-69	0.39073649193073123
2117	70-74	0.3640434618517645
2117	75-79	0.3859937366706063
2117	80-84	0.5263585861770395
2117	85-89	0.45382905672423135
2117	90-94	0.3825276974694063
2117	95-99	0.08886366766808607
2117	100-104	0.8015756750807981
2117	105-109	0.6134815283867923
2117	110-114	0.6169267086625254
2117	115-119	0.9362555404618149
2117	120-124	0.8028891476942306
2117	125-129	0.5803806545458841
2117	130-134	0.8801589113141048
2117	135-139	0.6045716083237522
2117	140-144	0.793330064682074
2117	145-149	0.9985746875390902
2117	150-151	1.369871019102078
1116	1	-0.048324744788907026
1116	2	-0.04150031538090815
1116	3	-0.5396614309304439
1116	4	-0.7170795434972135
1116	5	-0.1571897322706164
1116	6	1.028804798535596
1116	7	-0.33930346561194114
1116	8	0.20161946658395635
1116	9	0.12483986647860945
1116	10-14	0.24311986327450086
1116	15-19	0.23570763460437405
1116	20-24	0.10526221451810613
1116	25-29	0.09956723156356873
1116	30-34	0.20579900991841527
1116	35-39	0.1314322736751734
1116	40-44	0.11293331411134488
1116	45-49	0.20770242645001957
1116	50-54	0.15690556834040592
1116	55-59	0.3528202499054345
1116	60-64	0.3015543099968738
1116	65-69	0.2869929412244687
1116	70-74	0.2802638232941561
1116	75-79	0.06363223789702488
1116	80-84	0.2774580439194807
1116	85-89	0.3654750950487795
1116	90-94	0.3409826863329144
1116	95-99	-0.2600457085947525
1116	100-104	0.6041779474876918
1116	105-109	0.3275732704714329
1116	110-114	0.32889998109760654
1116	115-119	0.7291075144860812
1116	120-124	0.49349578071648637
1116	125-129	0.3233376028669568
1116	130-134	0.45110349202777655
1116	135-139	0.6374760225070304
1116	140-144	0.6287735975323585
1116	145-149	0.5141274237312601
1116	150-151	0.6479297934308335
2118	1	0.6042601185119594
2118	2	0.3758415201733243
2118	3	0.11623253539119816
2118	4	0.03773028795846045
2118	5	0.14352014215381814
2118	6	1.232524520179389
2118	7	0.5412855877786527
2118	8	0.16561571380941587
2118	9	0.14283462378138978
2118	10-14	0.2649638078575123
2118	15-19	0.2027046267911743
2118	20-24	0.09474956415922975
2118	25-29	0.25756259907360146
2118	30-34	0.2861977803508182
2118	35-39	0.07483672157746213
2118	40-44	0.0958124244291838
2118	45-49	0.20848852592905587
2118	50-54	0.1081544845869189
2118	55-59	0.27921598455682073
2118	60-64	0.24410467116328505
2118	65-69	0.3077503609631549
2118	70-74	0.2703484132730196
2118	75-79	0.1925070920193548
2118	80-84	0.21926542691859652
2118	85-89	0.1290959631194326
2118	90-94	0.2189604595341379
2118	95-99	0.3404582208856297
2118	100-104	0.6150297205211004
2118	105-109	0.30209681302957847
2118	110-114	0.4743607257030007
2118	115-119	0.7722379793368717
2118	120-124	0.4055520519942917
2118	125-129	0.2912820434976098
2118	130-134	0.3113810346215118
2118	135-139	0.4760853093527402
2118	140-144	0.29802428846493
2118	145-149	0.1689302886542592
2118	150-151	0.9434320575539488
1119	1	1.0983009914594746
1119	2	0.3273127024887117
1119	3	0.09987635483375357
1119	4	0.017371889067426594
1119	5	0.1037616503788783
1119	6	1.2134309341051264
1119	7	0.22943467954129915
1119	8	0.13405432732069755
1119	9	0.07846869746592944
1119	10-14	0.19968969456131447
1119	15-19	0.15798576178816148
1119	20-24	-0.03226998074993759
1119	25-29	0.16198611945415564
1119	30-34	0.1638433345665078
1119	35-39	0.09520685688372055
1119	40-44	0.062379256010117956
1119	45-49	0.1222217436108437
1119	50-54	0.07477818489226706
1119	55-59	0.24131381085182824
1119	60-64	0.3535279548007111
1119	65-69	0.08229991936164538
1119	70-74	0.204831058993463
1119	75-79	0.3026978263965745
1119	80-84	0.1942455052352443
1119	85-89	0.2359233330441839
1119	90-94	0.20891020954346118
1119	95-99	0.2747750960373736
1119	100-104	0.5532046021747163
1119	105-109	0.24535703806652975
1119	110-114	0.24732465914492963
1119	115-119	0.7154756885901037
1119	120-124	0.4801005526042843
1119	125-129	0.29676078345995194
1119	130-134	0.5693624517961737
1119	135-139	0.31058573599410266
1119	140-144	0.5645787288091881
1119	145-149	0.49193814550424264
1119	150-151	0.7672494500146456
2119	1	0.670965872310191
2119	2	0.3220935854688278
2119	3	0.12310819229653447
2119	4	0.15576113901473576
2119	5	0.09706109515791184
2119	6	1.2228054690488932
2119	7	0.50426880740293
2119	8	0.2950743997720906
2119	9	0.08433052844664246
2119	10-14	0.16364987126520703
2119	15-19	0.15492031083466884
2119	20-24	0.07768324645319069
2119	25-29	0.10856370506695612
2119	30-34	0.13150573345268413
2119	35-39	0.1686435937545312
2119	40-44	-0.1535200990786052
2119	45-49	0.16412869141368702
2119	50-54	0.13272736304737975
2119	55-59	0.2954001632428529
2119	60-64	0.20751572775439087
2119	65-69	0.22012391062033743
2119	70-74	0.21406102075023625
2119	75-79	-0.025302629046414893
2119	80-84	0.26589617646433794
2119	85-89	0.2989244826382276
2119	90-94	0.2764173982516738
2119	95-99	0.264054707073079
2119	100-104	0.7019032437360906
2119	105-109	0.41662278006405984
2119	110-114	0.4294517177522508
2119	115-119	0.832178011648999
2119	120-124	0.4279555259718535
2119	125-129	0.37678391516987375
2119	130-134	0.44847160130631636
2119	135-139	0.22243993830467446
2119	140-144	0.2669950736194835
2119	145-149	0.309795655769193
2119	150-151	0.23230431291731435
1118	1	0.4417805504651824
1118	2	0.3358633293894471
1118	3	0.12325379551808169
1118	4	0.1527484800861174
1118	5	0.1590916682337422
1118	6	1.2401765860056315
1118	7	0.3162518400142247
1118	8	0.5305035568284566
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2111	1	1.0293667019792423
2111	2	0.39586472779752313
2111	3	0.11122010172930175
2111	4	0.17170983210212398
2111	5	0.10263636502494933
2111	6	0.9616568494140267
2111	7	0.4423804026376388
2111	8	-0.08192672179858107
2111	9	-0.05281203344978991
2111	10-14	-0.11593044392431295
2111	15-19	-0.09980377323727652
2111	20-24	-0.06816758208707085
2111	25-29	0.021592753491390226
2111	30-34	0.01447636198609814
2111	35-39	-0.10665981101192301
2111	40-44	-0.11507980613431812
2111	45-49	-0.08878705486603877
2111	50-54	-0.26628149338110063
2111	55-59	-0.2030236098661149
2111	60-64	-0.13116519434939988
2111	65-69	-0.2583803111541485
2111	70-74	-0.16516681077351336
2111	75-79	-0.28035268777817635
2111	80-84	-0.18349878282141674
2111	85-89	-0.24243913224991331
2111	90-94	-0.5090553871931931
2111	95-99	-0.34972760208710696
2111	100-104	-0.23891957812083575
2111	105-109	-0.21193619363012317
2111	110-114	-0.45552647341189356
2111	115-119	-1.7873907550165882
2111	120-124	-0.8434097436926962
2111	125-129	-0.6490405579723628
2111	130-134	-0.4240802198437663
2111	135-139	-0.3373529051015822
2111	140-144	-0.7685546903479619
2111	145-149	-1.1952502951139792
2111	150-151	-2.732697166140923
2110	1	1.3580677539602846
2110	2	0.3723758061385283
2110	3	0.09218985060236662
2110	4	0.14797389798934546
2110	5	0.10113083369326148
2110	6	1.1778130914929292
2110	7	0.2841112418792022
2110	8	0.07499152976493662
2110	9	-0.021899334400714565
2110	10-14	-8.231670379501566E-4
2110	15-19	-0.08182988331530794
2110	20-24	-0.10842730898033182
2110	25-29	-0.13844297714727816
2110	30-34	0.12236589667638498
2110	35-39	-0.14091876287967864
2110	40-44	-0.14686930365305528
2110	45-49	-0.08268541642190996
2110	50-54	-0.19822961023426444
2110	55-59	-0.34270201975696324
2110	60-64	-0.00481449458848715
2110	65-69	-0.0012616491957189169
2110	70-74	-0.035441518780622516
2110	75-79	-0.11558523253064124
2110	80-84	-0.08820688327410409
2110	85-89	-0.06274906107346823
2110	90-94	-0.09907766379112815
2110	95-99	-0.3708369225706818
2110	100-104	0.15485494211372242
2110	105-109	0.0197406642948863
2110	110-114	-0.4001408718922903
2110	115-119	-6.463903484894349
2110	120-124	-2.7684311717837105
2110	125-129	-0.12965369458293452
2110	130-134	-0.4352824739760237
2110	135-139	-0.5232160855736154
2110	140-144	-0.3953827321016803
2110	145-149	-0.3813407174693637
2110	150-151	-0.7172657093442893
2109	1	1.149896527962035
2109	2	0.41760963186217737
2109	3	0.09336026142554488
2109	4	0.10044203017486097
2109	5	0.1010197486595601
2109	6	1.1394549566531893
2109	7	0.6485800557013945
2109	8	0.06286955272550188
2109	9	0.04025652357399423
2109	10-14	0.08552616041307459
2109	15-19	0.0701108076761372
2109	20-24	-0.25568786932633714
2109	25-29	-0.05806184676015391
2109	30-34	-0.013443403882604343
2109	35-39	-0.18898932220228204
2109	40-44	0.007909539044156588
2109	45-49	-0.15186709552182265
2109	50-54	0.03283080513394765
2109	55-59	-0.335731882959287
2109	60-64	-0.21999991323607304
2109	65-69	-0.21260598372899864
2109	70-74	-0.20385489124424083
2109	75-79	-0.14235218771113267
2109	80-84	-0.18507137820472508
2109	85-89	-0.29609995529617095
2109	90-94	-0.33503045312450297
2109	95-99	-0.18737133895945846
2109	100-104	-0.0032256314064866842
2109	105-109	-0.13688909141495031
2109	110-114	-0.845548368430336
2109	115-119	-4.653074584917437
2109	120-124	-1.264539579431748
2109	125-129	-0.5829031799874187
2109	130-134	-0.5326460006942142
2109	135-139	-0.6564544057504591
2109	140-144	-0.38890708914978234
2109	145-149	-0.21674295046762637
2109	150-151	-1.3988722494638282
2108	1	0.9083845479225943
2108	2	0.19707494047761998
2108	3	0.10437165319098796
2108	4	0.01166659634776579
2108	5	-0.005699573657324208
2108	6	0.9724496029203209
2108	7	-6.94903590897411E-4
2108	8	-0.24741947533185282
2108	9	-0.26522642447117306
2108	10-14	-0.11048108799624146
2108	15-19	-0.4038722987248704
2108	20-24	-0.4722669941099369
2108	25-29	-0.3372302348308054
2108	30-34	-5.65500647332771
2108	35-39	-0.8696742505346009
2108	40-44	-0.685504802162221
2108	45-49	-0.2286901655582625
2108	50-54	-0.1968024885371733
2108	55-59	-0.27194849138456334
2108	60-64	-0.44976452639098596
2108	65-69	-0.3871591978199831
2108	70-74	-0.3165049530549666
2108	75-79	-0.5005138534495117
2108	80-84	-0.3606181453880666
2108	85-89	-0.5423843996908957
2108	90-94	-0.618936677694677
2108	95-99	-0.5231725729364172
2108	100-104	-0.4041932935174515
2108	105-109	-0.5130605983899272
2108	110-114	-0.6882234182345073
2108	115-119	-0.5810540788339935
2108	120-124	-0.7217662131540052
2108	125-129	-0.4030450849403593
2108	130-134	-0.574370755588923
2108	135-139	-1.3440005506978778
2108	140-144	-1.1260757505141257
2108	145-149	-0.9743861572947452
2108	150-151	-1.5006004991082946
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	1.0
17	3.0
18	4.0
19	14.0
20	90.0
21	208.0
22	481.0
23	945.0
24	1440.0
25	2159.0
26	2712.0
27	3792.0
28	5425.0
29	7214.0
30	9875.0
31	13789.0
32	18884.0
33	25538.0
34	36633.0
35	59768.0
36	122901.0
37	450379.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.13000429322743373	13.682381667918857	85.37619405388	0.811419984973704
2	53.787703868516616	0.04342373165970488	30.618322453349005	15.550549946474677
3	0.12449885602737137	0.05483722004156084	14.144591843160303	85.67607208077077
4	0.5382706072500577	0.275366805902479	14.009597825402489	85.17676476144499
5	55.065883377762844	30.71304128796625	14.023766293738587	0.1973090405323146
6	44.92086648055246	39.5687275665918	0.3954052181944123	15.115000734661322
7	14.031768854558049	45.73424151466174	0.8070779370710104	39.4269116937092
8	54.883661132218045	14.415760584370604	30.320391049726076	0.38018723368527113
9	39.474533490061084	29.656178501710713	0.5262011712600492	30.34308683696816
10-14	12.446081106609853	51.622184672865814	21.247691064419303	14.684043156105037
15-19	34.048508637518104	18.34966205579228	24.3415860288407	23.26024327784891
20-24	26.60405428097647	28.387759492873787	11.014987090951072	33.99319913519867
25-29	23.986455993786866	40.26870237820365	13.60427467937281	22.14056694863668
30-34	16.878896328792425	16.82820469763439	38.31174828404106	27.981150689532125
35-39	32.11251862891207	19.410434289792406	22.128340085220714	26.34870699607481
40-44	8.44514178963498	32.195272979156606	33.15106735794799	26.20851787326043
45-49	9.232882391217649	26.683791272223505	29.573791482126737	34.509534854432104
50-54	21.73395814529502	33.39896307802103	15.403906299196072	29.463172477487877
55-59	17.518812577401818	31.381871707143006	44.32327197162108	6.776043743834093
60-64	23.145190067378937	25.519116937092	27.11595579437879	24.21973720115027
65-69	14.73769967045192	41.71144602338322	23.426906446128335	20.123947860036523
70-74	17.451879683465922	13.951034822946621	37.16431225205181	31.43277324153565
75-79	28.21726559056275	30.243304086815975	20.700945614071912	20.83848470854936
80-84	3.309150731512773	30.647682668289917	39.492217837576874	26.55094876262043
85-89	25.821403832833063	31.47695787242081	26.41243886568292	16.289199429063203
90-94	12.43209630360404	50.48967800843811	24.91491047627044	12.163315211687411
95-99	10.97497428685376	41.904373333893076	34.326682899183474	12.79396948006969
100-104	14.783773430448562	41.37861820700657	17.825061396696125	26.012546965848742
105-109	19.209819273315002	33.04136667996054	32.58519447534686	15.163619571377595
110-114	39.61432904431057	14.976831930480047	17.198736382527656	28.210102642681722
115-119	13.948935790600533	47.85103692197897	15.379793665120378	22.82023362230012
120-124	3.1077748158099117	20.65529166054449	47.17449255893033	29.062440964715265
125-129	22.924608005709366	32.57679834596251	6.239977120547428	38.258616527780696
130-134	18.170194790201716	47.54190193320879	27.736324804265234	6.551578472324258
135-139	38.816171994710444	8.683381960916018	36.50406162758968	15.996384416783863
140-144	14.465192796120988	45.107864024684616	17.304580088579165	23.122363090615227
145-149	29.859810877185616	26.776699691442246	28.873606767280286	14.489882664091855
150-151	40.850055624357175	22.394116937092	16.356709556894273	20.399117881656554
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.5
26	1.0
27	1.5
28	3.5
29	5.5
30	5.5
31	10.5
32	47.0
33	167.0
34	681.0
35	5848.5
36	134383.0
37	143817.0
38	15958.0
39	1544.5
40	953.5
41	4241.5
42	39718.0
43	44484.0
44	10814.0
45	3744.5
46	6472.5
47	50986.0
48	53916.0
49	18937.0
50	106095.0
51	105603.0
52	11458.0
53	1299.0
54	283.0
55	197.0
56	137.0
57	52.0
58	46.5
59	61.0
60	54.5
61	49.5
62	30.0
63	11.5
64	20.0
65	24.0
66	8.0
67	0.5
68	1.5
69	1.0
70	1.0
71	11.0
72	36.5
73	29.5
74	3.0
75	0.0
76	0.5
77	0.5
78	0.0
79	0.5
80	0.5
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.2165781574694066
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	762256.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	1.4664556765601051
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.14573639566515	1.0726498035359224
2	9.779677624263735	0.28682927534058794
3	3.2971727412561456	0.14505473049042952
4	2.169736822455756	0.1272729151972691
5	1.321786512598715	0.09691706673004852
6	0.9377683880967165	0.08251174656138298
7	0.6933000615423023	0.07116856675657257
8	0.5489482479204151	0.06440066194404932
9	0.4408741392238251	0.058187014570199556
>10	4.3363116639526185	1.4648108547558976
>50	1.2415104368037726	1.3406623710188204
>100	1.7363153210371602	4.910396914910499
>500	0.15293969149031983	1.536181130500211
>1k	0.12595033416849866	3.772377468698302
>5k	0.03598580976242819	3.0190629500486796
>10k+	0.03598580976242819	81.95151652894113
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	258421	33.90212736928276	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	191330	25.100491173568983	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	91622	12.019846350832267	No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	79806	10.469710963245944	No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	6767	0.8877594928737851	No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG	5936	0.7787410003988161	No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC	5133	0.6733958145295019	No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	5048	0.662244705190907	No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	4845	0.6356132322999097	No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC	2683	0.3519814865347075	No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG	2618	0.34345416762872316	No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	2601	0.3412239457610042	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG	2428	0.31852815851892274	No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC	2094	0.2747108582943263	No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	1780	0.2335173485023404	No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	1713	0.22472765055309504	No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCCTCCGCGGCTATATTGA	1678	0.22013601729602653	No Hit
AGTTTGGAAACTCCGAGCCCACGAGACATACTGTTTGTGATCAATTTGCC	1422	0.1865514997586113	No Hit
TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	1340	0.17579395898490796	No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	1292	0.1694968619466426	No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA	1071	0.14050397766629585	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA	1029	0.13499401775781364	No Hit
AGTTTAGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	979	0.12843454167628723	No Hit
TTCCATATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	972	0.12751621502487354	No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTGCGCTGAATTACTTACACAG	893	0.1171522428160618	No Hit
TGCCGCCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	837	0.10980562960475221	No Hit
AGTTTGTAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	832	0.10914968199659957	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10-11	0.0	0.0	0.0
12-13	0.0	0.0	0.0
14-15	0.0	0.0	0.0
16-17	0.0	0.0	0.0
18-19	0.0	0.0	2.6237904326105666E-4
20-21	0.0	0.0	0.017907369702567115
22-23	0.0	0.0	0.02459803530572406
24-25	0.0	0.0	0.025122793392246174
26-27	0.0	0.0	0.025713146239583554
28-29	0.0	0.0	0.05227902436976554
30-31	0.0	0.0	0.0525414034130266
32-33	0.0	0.0	0.058182552843139314
34-35	0.0	0.0	0.06402048655569782
36-37	0.0	0.0	0.07333494259146534
38-39	0.0	0.0	0.17435087424697215
40-41	0.0	0.0	0.2350260280010915
42-43	0.0	0.0	0.29019122184672863
44-45	0.0	0.0	0.346143552822149
46-47	0.0	0.0	0.4847452824248022
48-49	0.0	0.0	0.5907464159022691
50-51	0.0	0.0	0.6916967527969606
52-53	0.0	0.0	0.9460076404777398
54-55	0.0	0.0	0.9462044247601855
56-57	0.0	0.0	0.9462700195210009
58-59	0.0	0.0	0.9468603723683382
60-61	0.0	0.0	0.948565836149535
62-63	0.0	0.0	0.9527639008417119
64-65	0.0	0.0	0.9529606851241578
66-67	0.0	0.0	0.9532230641674189
68-69	0.0	0.0	0.954010201297202
70-71	0.0	0.0	0.954010201297202
72-73	0.0	0.0	0.9541413908188325
74-75	0.0	0.0	0.9549285279486157
76-77	0.0	0.0	0.9668011796561785
78-79	0.0	0.0	0.9668667744169938
80-81	0.0	0.0	0.9673259377427006
82-83	0.0	0.0	0.9673915325035158
84-85	0.0	0.0	0.9689658067630822
86-87	0.0	0.0	0.9693593753279738
88-89	0.0	0.0	0.9693593753279738
90-91	0.0	0.0	0.9693593753279738
92-93	0.0	0.0	0.9693593753279738
94-95	0.0	0.0	0.9693593753279738
96-97	0.0	0.0	0.9693593753279738
98-99	0.0	0.0	0.970146512457757
100-101	0.0	0.0	0.9709336495875401
102-103	0.0	0.0	0.971392812913247
104-105	0.0	0.0	0.9716551919565081
106-107	0.0	0.0	0.9717207867173233
108-109	0.0	0.0	0.9717863814781386
110-111	0.0	0.0	0.9718519762389538
112-113	0.0	0.0	0.9718519762389538
114-115	0.0	0.0	0.9718519762389538
116-117	0.0	0.0	0.9719175709997692
118-119	0.0	0.0	0.9726391133687371
120-121	0.0	0.0	0.9750661195189019
122-123	0.0	0.0	1.003665435234357
124-125	0.0	0.0	1.0306248819294306
126-127	0.0	0.0	1.0308872609726916
128-129	0.0	0.0	1.0335766461661173
130-131	0.0	0.0	1.033773430448563
132-133	0.0	0.0	1.0339046199701938
134-135	0.0	0.0	1.0339046199701938
136-137	0.0	0.0	1.0339046199701938
138-139	0.0	0.0	1.0340358094918243
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCCACA	40	0.0	148.26505	1
TTCCGGA	10	0.0066338177	148.26505	1
TGCCGCC	80	0.0	148.26505	1
TTCCGAG	10	0.0066338177	148.26505	1
TTTCAGA	20	1.9067593E-6	148.26505	1
GGTTTGG	10	0.0066338177	148.26505	1
ACCCGCA	10	0.0066338177	148.26505	1
TCCCGGA	315	0.0	148.26505	1
TCCCGCG	10	0.0066338177	148.26505	1
TCCCGAA	165	0.0	148.26505	1
AGTTTTG	25	3.248533E-8	148.26505	1
AGTTTGT	165	0.0	148.26505	1
AGTTTGA	20	1.9067593E-6	148.26505	1
AGTTTCG	40	0.0	148.26505	1
TTCCGCA	15	1.1254809E-4	148.26503	1
CTCCAGA	15	1.1254809E-4	148.26503	1
CGGGTGT	15	1.1254809E-4	148.26503	1
CCCCGCA	15	1.1254809E-4	148.26503	1
AGTGTGG	15	1.1254809E-4	148.26503	1
CGTGTGT	55	0.0	148.26503	1
>>END_MODULE