FastQCFastQC Report
Wed 17 Jun 2015
000000000-AG1W8 l01n01 mis14.3410000000d249.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG1W8 l01n01 mis14.3410000000d249.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences762256
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG25842133.90212736928276No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC19133025.100491173568983No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG9162212.019846350832267No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA7980610.469710963245944No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG67670.8877594928737851No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG59360.7787410003988161No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC51330.6733958145295019No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC50480.662244705190907No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG48450.6356132322999097No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC26830.3519814865347075No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG26180.34345416762872316No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG26010.3412239457610042No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG24280.31852815851892274No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC20940.2747108582943263No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA17800.2335173485023404No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC17130.22472765055309504No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCCTCCGCGGCTATATTGA16780.22013601729602653No Hit
AGTTTGGAAACTCCGAGCCCACGAGACATACTGTTTGTGATCAATTTGCC14220.1865514997586113No Hit
TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG13400.17579395898490796No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG12920.1694968619466426No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA10710.14050397766629585No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA10290.13499401775781364No Hit
AGTTTAGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA9790.12843454167628723No Hit
TTCCATATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC9720.12751621502487354No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTGCGCTGAATTACTTACACAG8930.1171522428160618No Hit
TGCCGCCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG8370.10980562960475221No Hit
AGTTTGTAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA8320.10914968199659957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCACA400.0148.265051
TTCCGGA100.0066338177148.265051
TGCCGCC800.0148.265051
TTCCGAG100.0066338177148.265051
TTTCAGA201.9067593E-6148.265051
GGTTTGG100.0066338177148.265051
ACCCGCA100.0066338177148.265051
TCCCGGA3150.0148.265051
TCCCGCG100.0066338177148.265051
TCCCGAA1650.0148.265051
AGTTTTG253.248533E-8148.265051
AGTTTGT1650.0148.265051
AGTTTGA201.9067593E-6148.265051
AGTTTCG400.0148.265051
TTCCGCA151.1254809E-4148.265031
CTCCAGA151.1254809E-4148.265031
CGGGTGT151.1254809E-4148.265031
CCCCGCA151.1254809E-4148.265031
AGTGTGG151.1254809E-4148.265031
CGTGTGT550.0148.265031
Produced by FastQC (version 0.11.2)