##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AG1W8 l01n01 mis13.3410000000d22f.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	861402
Sequences flagged as poor quality	0
Sequence length	151
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08788579548225	34.0	34.0	34.0	31.0	34.0
2	33.210784279581425	34.0	34.0	34.0	32.0	34.0
3	33.436114613153904	34.0	34.0	34.0	33.0	34.0
4	33.46076396386356	34.0	34.0	34.0	34.0	34.0
5	33.643451025189165	34.0	34.0	34.0	34.0	34.0
6	36.238848992688666	38.0	37.0	38.0	34.0	38.0
7	36.61933800943114	38.0	37.0	38.0	34.0	38.0
8	36.406615029916345	38.0	37.0	38.0	34.0	38.0
9	37.04446356056754	38.0	37.0	38.0	35.0	38.0
10-14	37.09212632429458	38.0	37.8	38.0	35.8	38.0
15-19	37.227224455016355	38.0	38.0	38.0	36.4	38.0
20-24	37.38261996141175	38.0	37.8	38.0	36.6	38.0
25-29	37.12837908432997	38.0	38.0	38.0	35.8	38.0
30-34	36.545771892798015	38.0	37.8	38.0	33.6	38.0
35-39	36.94676353200945	38.0	37.6	38.0	35.6	38.0
40-44	36.72095003262124	38.0	37.6	38.0	34.8	38.0
45-49	36.89330811862522	38.0	37.6	38.0	35.2	38.0
50-54	36.72016131840883	38.0	37.2	38.0	34.4	38.0
55-59	36.028525125318964	38.0	36.6	38.0	32.0	38.0
60-64	36.18227401375896	38.0	37.0	38.0	33.2	38.0
65-69	36.424204726712965	38.0	37.0	38.0	33.8	38.0
70-74	36.455586358053495	38.0	37.0	38.0	34.2	38.0
75-79	36.06146375327664	38.0	36.6	38.0	32.4	38.0
80-84	36.49346228590135	38.0	37.0	38.0	34.0	38.0
85-89	36.13378445835974	38.0	36.8	38.0	32.6	38.0
90-94	36.13412320844391	38.0	37.0	38.0	32.8	38.0
95-99	35.99221362383649	38.0	37.0	38.0	32.0	38.0
100-104	35.37640497700261	38.0	36.2	38.0	29.0	38.0
105-109	35.5999623868995	38.0	36.6	38.0	30.2	38.0
110-114	35.975580971486025	38.0	37.0	38.0	32.2	38.0
115-119	35.212969089925494	38.0	36.0	38.0	28.4	38.0
120-124	35.39913722048475	38.0	36.4	38.0	29.4	38.0
125-129	35.366730283886035	38.0	36.4	38.0	29.4	38.0
130-134	35.01408378434227	37.8	35.2	38.0	28.2	38.0
135-139	34.29543325880367	37.4	34.4	38.0	24.8	38.0
140-144	35.1154875424018	37.8	35.6	38.0	28.2	38.0
145-149	34.750165660167966	37.6	34.8	38.0	26.6	38.0
150-151	33.07443795115405	36.0	32.0	37.5	22.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.3771879203659978
1101	2	0.47327654409754416
1101	3	0.16244482202186106
1101	4	0.10598734482471883
1101	5	0.17507035111237457
1101	6	1.0494985533557468
1101	7	-0.2103701202197641
1101	8	0.27491760638419294
1101	9	0.2392041139154344
1101	10-14	0.2711825980085152
1101	15-19	0.2665823106289267
1101	20-24	0.2110894641021872
1101	25-29	0.27410169646989146
1101	30-34	0.2727954393024774
1101	35-39	0.1477091875740939
1101	40-44	0.22497678202820026
1101	45-49	0.3303204568383862
1101	50-54	0.33623909038627886
1101	55-59	0.6065825208097237
1101	60-64	0.5651023851279717
1101	65-69	0.41449333294376345
1101	70-74	0.25357999611940585
1101	75-79	0.26695205832211144
1101	80-84	0.3462915535898148
1101	85-89	0.410577774777785
1101	90-94	0.48223840249764294
1101	95-99	0.5804875831026735
1101	100-104	0.8892072278231993
1101	105-109	0.5308179419244183
1101	110-114	0.39145706538678837
1101	115-119	0.9215720154796045
1101	120-124	0.6345970462139121
1101	125-129	0.4937391899247956
1101	130-134	0.6262517240376368
1101	135-139	0.8502148944830807
1101	140-144	0.6319041570961446
1101	145-149	0.6962770888088841
1101	150-151	0.680146864037404
1102	1	0.4615106852510067
1102	2	0.3160078570596738
1102	3	0.11576349292507615
1102	4	0.1318348628627959
1102	5	0.20680743366763465
1102	6	1.0604626413688791
1102	7	0.41551757659892985
1102	8	0.24830539542465857
1102	9	0.22161700754867297
1102	10-14	0.28378663828566175
1102	15-19	0.26192712199969037
1102	20-24	0.18477379181879172
1102	25-29	0.2773970211420149
1102	30-34	0.27554816765437096
1102	35-39	0.29738494913479485
1102	40-44	0.2457398398826527
1102	45-49	0.27442931375041724
1102	50-54	0.3311831109429164
1102	55-59	0.46321602419666874
1102	60-64	0.5315651336822498
1102	65-69	0.4728226201509287
1102	70-74	0.3260990062657996
1102	75-79	0.26773895093376865
1102	80-84	0.47717152205828484
1102	85-89	0.3596720573273231
1102	90-94	0.49928916036116533
1102	95-99	0.45309313216116465
1102	100-104	0.9481316195998843
1102	105-109	0.6042975871680483
1102	110-114	0.291122807177139
1102	115-119	0.9507793968101268
1102	120-124	0.609599630085043
1102	125-129	0.630773357840198
1102	130-134	0.8451187355970973
1102	135-139	0.9421484974695389
1102	140-144	0.6278551064153817
1102	145-149	0.8525193725056326
1102	150-151	1.2213468968716725
1103	1	0.8830019185017193
1103	2	0.32713422952713245
1103	3	0.25766165318169243
1103	4	0.2743600511315023
1103	5	0.18080551343653894
1103	6	1.203798781366885
1103	7	0.5231622224782768
1103	8	0.23758129281209506
1103	9	0.12406167435145932
1103	10-14	0.24330249706599716
1103	15-19	0.3014714298981147
1103	20-24	0.1551004504648077
1103	25-29	0.22773539016048971
1103	30-34	0.4114631712737875
1103	35-39	0.1266189659106658
1103	40-44	0.43586975077245427
1103	45-49	0.24127765430289116
1103	50-54	0.26618214070313684
1103	55-59	0.5175041972266996
1103	60-64	0.5870651109026994
1103	65-69	0.4168324609134828
1103	70-74	0.39739661340136223
1103	75-79	0.39964526120465393
1103	80-84	0.4145278126712526
1103	85-89	0.3833067274542401
1103	90-94	0.33859922706304246
1103	95-99	0.48540316342819523
1103	100-104	0.8165982526315787
1103	105-109	0.5251608474774727
1103	110-114	0.42732567672459965
1103	115-119	1.125226925398465
1103	120-124	0.8523914571225006
1103	125-129	0.5809918558414964
1103	130-134	0.7024749249676532
1103	135-139	0.8535841655091616
1103	140-144	0.92233790111095
1103	145-149	1.0078552020269171
1103	150-151	0.9760161125973212
2116	1	0.7101329760864914
2116	2	0.3068568541921124
2116	3	0.047769503975054306
2116	4	-0.35436363621425215
2116	5	0.027547466368993412
2116	6	0.9346154802386408
2116	7	-0.16772655084721322
2116	8	0.3114950017419176
2116	9	0.11666065062984643
2116	10-14	0.244216298175985
2116	15-19	0.30566239241557724
2116	20-24	0.13303418857922367
2116	25-29	0.19531364329417755
2116	30-34	0.33698058897689975
2116	35-39	0.18255288142840698
2116	40-44	0.15735214696976385
2116	45-49	0.34226467777057223
2116	50-54	0.30053567076865306
2116	55-59	0.5272578326906796
2116	60-64	0.503163559319205
2116	65-69	0.4381182872564935
2116	70-74	0.365011286396566
2116	75-79	0.41670523570726914
2116	80-84	0.5055764924947468
2116	85-89	0.5328067109313821
2116	90-94	0.4444304051777266
2116	95-99	0.6277518444691665
2116	100-104	0.8725243415315163
2116	105-109	0.483306669541939
2116	110-114	0.4314529479656102
2116	115-119	0.9622085989380622
2116	120-124	0.7662420313353664
2116	125-129	0.6558048953346116
2116	130-134	0.8467821040632799
2116	135-139	0.779146594960892
2116	140-144	0.9391319097480704
2116	145-149	0.9107335189910231
2116	150-151	1.7506804811371026
1117	1	0.4133784163162346
1117	2	0.3654485172479838
1117	3	0.1661952246269891
1117	4	0.11995798972402838
1117	5	0.178198321251422
1117	6	1.1464651336722582
1117	7	0.2653001802669124
1117	8	0.3551758269386909
1117	9	0.2231893217584755
1117	10-14	0.26232854557797225
1117	15-19	0.23771497963910804
1117	20-24	0.14705845313353194
1117	25-29	0.28340222069633825
1117	30-34	0.43804335514170845
1117	35-39	0.16714073877459157
1117	40-44	0.21402167422883167
1117	45-49	0.358604479688573
1117	50-54	0.26773140680327856
1117	55-59	0.6556256543916348
1117	60-64	0.6040505532993379
1117	65-69	0.43514761814429903
1117	70-74	0.41996123304091526
1117	75-79	0.3806395458487657
1117	80-84	0.4384836816963045
1117	85-89	0.4725130655130201
1117	90-94	0.4414948624574464
1117	95-99	-0.2852137204487448
1117	100-104	0.6186478882734505
1117	105-109	0.6866339311276874
1117	110-114	0.6230195289547282
1117	115-119	1.20156347926293
1117	120-124	0.8814065364318466
1117	125-129	0.6712465553756459
1117	130-134	0.8376195144418972
1117	135-139	0.6555134743490711
1117	140-144	0.7480463009116392
1117	145-149	-0.622570857855564
1117	150-151	0.3710493713534575
2117	1	-29.95895327498097
2117	2	-10.234614053342778
2117	3	-2.3955278351102542
2117	4	-0.9027030809400856
2117	5	-0.06962275513949123
2117	6	1.1693003061214071
2117	7	-0.1735604870466645
2117	8	-0.29120488859313554
2117	9	0.11555067222271731
2117	10-14	0.4192593873998689
2117	15-19	0.43760265305596846
2117	20-24	0.23012701035116123
2117	25-29	0.41191266945474325
2117	30-34	0.6326163285350646
2117	35-39	0.35912392602150334
2117	40-44	0.1685794291818894
2117	45-49	0.5173544642226773
2117	50-54	0.3101537370805474
2117	55-59	0.6271390672992041
2117	60-64	0.5984353730377094
2117	65-69	0.5446208762868707
2117	70-74	0.6140796858858479
2117	75-79	0.47264226431115475
2117	80-84	0.64104836890521
2117	85-89	0.3038626106389657
2117	90-94	0.5392422328134856
2117	95-99	0.09743734599097564
2117	100-104	1.1060540544814472
2117	105-109	0.8578336073160386
2117	110-114	0.721286464993085
2117	115-119	1.3202878169249388
2117	120-124	1.0684698016622107
2117	125-129	0.8503558435733041
2117	130-134	1.0166977824738126
2117	135-139	0.4365476919780704
2117	140-144	0.9759303367500962
2117	145-149	1.129254117708328
2117	150-151	1.3197560690579664
1116	1	-0.3574459739918048
1116	2	-0.27951086498136846
1116	3	-0.7972422074543601
1116	4	-1.171012087783481
1116	5	-0.3074452710564515
1116	6	0.9652906563123267
1116	7	-0.670541879525473
1116	8	-0.05411579264416133
1116	9	0.02736839264292712
1116	10-14	0.24547307956223108
1116	15-19	0.2546519673729435
1116	20-24	0.07775752893713417
1116	25-29	0.06461618272145131
1116	30-34	0.25938881927795165
1116	35-39	0.19035362260923705
1116	40-44	-0.028549407836628404
1116	45-49	0.2068800710497385
1116	50-54	0.1299839122038975
1116	55-59	0.4849783456524861
1116	60-64	0.4640368864230666
1116	65-69	0.37229816338847144
1116	70-74	0.35071940048838
1116	75-79	0.29020454162959197
1116	80-84	0.3773102949020455
1116	85-89	0.4001747225386296
1116	90-94	0.5761441837156696
1116	95-99	-0.33939159248439665
1116	100-104	0.9061350779939872
1116	105-109	0.45174634833944083
1116	110-114	0.5776936857192965
1116	115-119	1.0583240970026395
1116	120-124	0.808109617163872
1116	125-129	0.5592219859363894
1116	130-134	0.6227579660087983
1116	135-139	0.5825905959053728
1116	140-144	0.6263595129214679
1116	145-149	0.6399612886948063
1116	150-151	0.6142623402676932
2118	1	0.6965020162413396
2118	2	0.49462853640282844
2118	3	0.14806345493231987
2118	4	0.048115541675819884
2118	5	0.09056828182369969
2118	6	1.1871695119413488
2118	7	0.630852242510727
2118	8	0.2815307087969501
2118	9	-0.004277470451881982
2118	10-14	0.23446666789147486
2118	15-19	0.1805724592550888
2118	20-24	0.1269359672066912
2118	25-29	0.21316941257210686
2118	30-34	0.38073346561207444
2118	35-39	0.09330191694561307
2118	40-44	0.07223751240138654
2118	45-49	0.2821856136498937
2118	50-54	0.20604998336405345
2118	55-59	0.6056844524858462
2118	60-64	0.2893880058668614
2118	65-69	0.4104050029289965
2118	70-74	0.4725670729780944
2118	75-79	0.4126830254566727
2118	80-84	0.26627127038174336
2118	85-89	0.27841947888315133
2118	90-94	0.46670496630040503
2118	95-99	0.5338901373258693
2118	100-104	0.9283438489184732
2118	105-109	0.366802472194081
2118	110-114	0.6163821468371609
2118	115-119	0.9831874920543768
2118	120-124	0.6113693178443711
2118	125-129	0.4963156246146099
2118	130-134	0.6501371100915208
2118	135-139	0.7261996945812754
2118	140-144	0.5447254545962465
2118	145-149	0.3807751106696031
2118	150-151	1.2583035205591742
1119	1	1.149566477517837
1119	2	0.30899991079034805
1119	3	0.12758510152507796
1119	4	-0.010008075383758808
1119	5	0.18202557061035662
1119	6	1.2758114297905365
1119	7	0.3751679107791688
1119	8	0.054884855949033806
1119	9	0.10061749928595276
1119	10-14	0.3197372468844861
1119	15-19	0.23933579668688765
1119	20-24	-0.30276593262694007
1119	25-29	0.1689903642732915
1119	30-34	0.39704051733438916
1119	35-39	0.2654227360094694
1119	40-44	0.1608989920124415
1119	45-49	0.2516507575967353
1119	50-54	0.0659867531900602
1119	55-59	0.49400686155781415
1119	60-64	0.44830147298806367
1119	65-69	0.3106916744971713
1119	70-74	0.4306305210256056
1119	75-79	0.5818125348625216
1119	80-84	0.3301168784928876
1119	85-89	0.4802367704654671
1119	90-94	0.4074044027576704
1119	95-99	0.376115703572502
1119	100-104	0.787190977849221
1119	105-109	0.354874639086745
1119	110-114	0.5197232645282881
1119	115-119	0.940692473275476
1119	120-124	0.7516820092890057
1119	125-129	0.5851302013679671
1119	130-134	0.6752930282663456
1119	135-139	0.29545715587065047
1119	140-144	0.7752321198468763
1119	145-149	0.7917192721865121
1119	150-151	1.1175792902520314
2119	1	0.586084256295095
2119	2	0.2124556701709821
2119	3	0.18867222037193443
2119	4	0.1787093986019812
2119	5	0.14992421214846274
2119	6	1.1700230109947682
2119	7	0.4545818202761751
2119	8	0.3803589639535261
2119	9	0.13925135210396178
2119	10-14	0.1841875523309895
2119	15-19	0.18081582585605105
2119	20-24	0.10173476385224234
2119	25-29	0.09337892542154691
2119	30-34	0.19897275580536444
2119	35-39	0.16345512774952908
2119	40-44	-0.14288994204691363
2119	45-49	0.1756498144023695
2119	50-54	0.12328057133694159
2119	55-59	0.4687100391621968
2119	60-64	0.2864519386902771
2119	65-69	0.23878707686127143
2119	70-74	0.28659688042632325
2119	75-79	-0.07503937925439885
2119	80-84	0.17702551527134602
2119	85-89	0.38811069681771215
2119	90-94	0.37686499164282594
2119	95-99	0.3478434522635965
2119	100-104	0.8420272506594628
2119	105-109	0.5553715627884444
2119	110-114	0.40869793364610985
2119	115-119	1.066873103731055
2119	120-124	0.5845191466940918
2119	125-129	0.5351959193091815
2119	130-134	0.6371023894704919
2119	135-139	0.3783962543340351
2119	140-144	0.30385457573287766
2119	145-149	0.504621821867957
2119	150-151	0.4171766859875703
1118	1	0.3009818426183841
1118	2	0.34967937713955166
1118	3	0.151970237711339
1118	4	0.20459776055810863
1118	5	0.21281691741041442
1118	6	1.3145750741834092
1118	7	0.4458512411980422
1118	8	0.8319795696623942
1118	9	0.3434405013476862
1118	10-14	0.24914571224663007
1118	15-19	0.2420531911381545
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2111	3	0.043798975268003915
2111	4	0.11842519167550591
2111	5	0.06975244899176403
2111	6	0.9881142383577171
2111	7	0.4901010168226776
2111	8	-0.08326094883474866
2111	9	-0.038395020613712916
2111	10-14	-0.12937676474206228
2111	15-19	-0.1508698784681144
2111	20-24	-0.1388933843639748
2111	25-29	-0.0941403274431778
2111	30-34	-0.054386792670726436
2111	35-39	-0.228122742006434
2111	40-44	-0.1701830105710016
2111	45-49	-0.2616973529579667
2111	50-54	-0.4774990654772324
2111	55-59	-0.2641032892852877
2111	60-64	-0.23960809430774077
2111	65-69	-0.3964246296215137
2111	70-74	-0.3858166405405399
2111	75-79	-0.44734148648205263
2111	80-84	-0.18609120164326498
2111	85-89	-0.3736818750315578
2111	90-94	-0.7047319559097218
2111	95-99	-0.5626932788861936
2111	100-104	-0.7597548504473011
2111	105-109	-0.4889362770410628
2111	110-114	-0.5930936526343373
2111	115-119	-3.058294026140615
2111	120-124	-1.3399424863341736
2111	125-129	-0.9125167273543795
2111	130-134	-0.6234225037379346
2111	135-139	-0.5874465237251343
2111	140-144	-1.163859226936232
2111	145-149	-1.6814629466845403
2111	150-151	-2.6507718006270338
2110	1	1.4162172570790545
2110	2	0.3838682198893508
2110	3	0.22523235973318378
2110	4	0.2361466065830129
2110	5	0.14071823065941658
2110	6	1.1761981963944592
2110	7	0.3500716953084577
2110	8	-0.284071300618173
2110	9	-0.1870704918155397
2110	10-14	-0.03872645703182087
2110	15-19	-0.14625702286091524
2110	20-24	-0.2149030967872747
2110	25-29	-0.16122307920485923
2110	30-34	-0.024849316628049678
2110	35-39	-0.28217388475557925
2110	40-44	-0.2644823255740434
2110	45-49	-0.11245524965548981
2110	50-54	-0.3167395909856836
2110	55-59	-0.6264284364736739
2110	60-64	-0.2160704376571374
2110	65-69	-0.25966103872391244
2110	70-74	-0.28253307135628347
2110	75-79	-0.4055166111146562
2110	80-84	-0.34401642471122074
2110	85-89	-0.41651855880345323
2110	90-94	-0.38878009458751706
2110	95-99	-0.6248052723632966
2110	100-104	-0.0734813151038125
2110	105-109	-0.2404838586424063
2110	110-114	-0.5193817416631319
2110	115-119	-6.886077342696002
2110	120-124	-3.23398111178264
2110	125-129	-0.6662383515569701
2110	130-134	-1.0383563545715546
2110	135-139	-0.9250963863802824
2110	140-144	-0.914344575371139
2110	145-149	-0.7275550983410568
2110	150-151	-1.2816952707476332
2109	1	1.329295062047855
2109	2	0.5627211352943604
2109	3	0.15602916416656143
2109	4	0.25665239988353505
2109	5	0.13145963801147786
2109	6	1.156676523067837
2109	7	0.7546387783103086
2109	8	0.058495215708035175
2109	9	-0.015814861610714104
2109	10-14	0.10774620893507603
2109	15-19	0.045905026205936394
2109	20-24	-0.3274403617605657
2109	25-29	-0.1290605058117933
2109	30-34	-0.09548360010098378
2109	35-39	-0.19577553194655195
2109	40-44	0.022099581743233898
2109	45-49	-0.26512847644976034
2109	50-54	-0.0937197744796947
2109	55-59	-0.6242072292932264
2109	60-64	-0.39354155182009265
2109	65-69	-0.4020648525921402
2109	70-74	-0.49999438465229673
2109	75-79	-0.2897557079022519
2109	80-84	-0.2299978171804895
2109	85-89	-0.36535174139299187
2109	90-94	-0.5652599498734787
2109	95-99	-0.29774413088495777
2109	100-104	-0.4596257528597363
2109	105-109	-0.4045885665876696
2109	110-114	-1.131851711383291
2109	115-119	-5.605062045478661
2109	120-124	-1.7984862032926898
2109	125-129	-0.909425004631494
2109	130-134	-1.0070677523143274
2109	135-139	-1.321354350845084
2109	140-144	-0.8247660227333213
2109	145-149	-0.6933695937426165
2109	150-151	-2.1109321038576248
2108	1	0.7720849175956843
2108	2	0.23503118921792066
2108	3	0.03801659512209454
2108	4	-0.021881823866443995
2108	5	-0.0247455076871006
2108	6	0.75273000054721
2108	7	0.16939100544527008
2108	8	-0.038987039372720744
2108	9	-0.22128331435872184
2108	10-14	-0.2506279497392754
2108	15-19	-0.3537134416301697
2108	20-24	-0.6889759368796291
2108	25-29	-0.39956785962122154
2108	30-34	-7.330673250975433
2108	35-39	-1.2300646199692267
2108	40-44	-0.8760087639448457
2108	45-49	-0.33117342587182463
2108	50-54	-0.4403745455997523
2108	55-59	-0.5680464294926466
2108	60-64	-0.7094549067578413
2108	65-69	-0.5776872039743353
2108	70-74	-0.4011261234575798
2108	75-79	-0.7459327766121504
2108	80-84	-0.472523171750872
2108	85-89	-0.7778111019198874
2108	90-94	-0.9196424529238953
2108	95-99	-0.7101447134168737
2108	100-104	-0.7650960424200761
2108	105-109	-0.8904427499277787
2108	110-114	-0.9811821932841269
2108	115-119	-0.9887595487258807
2108	120-124	-1.0645384999641223
2108	125-129	-0.7343778379145292
2108	130-134	-1.359106482814596
2108	135-139	-1.8890652890625859
2108	140-144	-1.9456812800838463
2108	145-149	-1.5797685231434215
2108	150-151	-1.7253681368677718
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	16.0
15	25.0
16	28.0
17	37.0
18	17.0
19	24.0
20	94.0
21	244.0
22	604.0
23	1154.0
24	1898.0
25	2782.0
26	3996.0
27	5609.0
28	8039.0
29	10972.0
30	14777.0
31	19949.0
32	27025.0
33	36791.0
34	52939.0
35	83997.0
36	162285.0
37	428097.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.11995472332104916	19.98058892904518	78.79672717004146	1.1027291775923154
2	20.784372453279655	30.351914669341372	27.735598477830326	21.128114399548643
3	0.1280470674551487	0.1247965525968131	20.66201378682659	79.08514259312145
4	0.5514266277533603	30.75880947571517	20.393033682299322	48.29673021423215
5	21.287389627607087	27.630421104199897	20.479984954759797	30.60220431343322
6	77.8564479766706	1.5619884792466237	0.5064998688185075	20.07506367526428
7	20.052542250888667	48.30497259119436	0.9915231216087262	30.65096203630825
8	20.709262342088827	51.375316054525065	27.581895560957605	0.3335260424285061
9	0.38565036997824476	41.197954033076314	30.94815196621322	27.468243630732225
10-14	26.092486434904956	26.289746250879382	29.51836656984776	18.0994007443679
15-19	25.470779032321726	34.29239774228525	12.742436168014471	27.494387057378557
20-24	25.612362172365515	14.109300883907864	34.70379683353417	25.574540110192455
25-29	39.98592991425606	30.820313860427532	8.431974850302183	20.76178137501422
30-34	24.429639123196836	15.985846329588277	30.42974128223524	29.15477326497965
35-39	39.828953264561726	4.491282815688842	14.501939860831529	41.177824058917906
40-44	18.336711546989676	30.87271680353656	22.62063473267998	28.169936916793787
45-49	9.83431661407798	16.756218351013814	41.64506235184037	31.76440268306784
50-54	20.663290774806654	22.117315724829986	21.673968716116285	35.545424784247075
55-59	25.24094441387413	18.182428181035103	38.53195140015927	18.044676004931496
60-64	24.1134336813706	22.066398731370487	16.554547122017365	37.26562046524155
65-69	30.069978941307312	22.117547904462725	21.51443809046183	26.29803506376814
70-74	15.97883450467958	14.636580829856443	35.64456548742631	33.74001917803767
75-79	26.245701774548934	30.02762937629585	29.28641911674224	14.440249732412974
80-84	16.617305276746514	18.274974982644572	27.694572336725475	37.413147403883436
85-89	25.41013371225049	39.74383621119988	34.244057942749144	0.6019721338004788
90-94	23.228782844711297	31.84557268267313	12.806332002944037	32.119312469671534
95-99	4.521698347577554	31.70508078690321	34.30948616325479	29.46373470226445
100-104	30.01146967385727	28.056702909907337	10.12823281116134	31.803594605074053
105-109	10.379544045637228	43.12163194420259	25.065486265413828	21.433337744746357
110-114	30.954908393525905	24.069110589480868	30.89953355111783	14.0764474658754
115-119	20.516181759503695	34.23015038274813	15.26578763457712	29.987880223171064
120-124	4.245079533133195	20.348385538923754	42.402176916236556	33.0043580117065
125-129	21.554164025623344	40.11527718765455	13.905052460988019	24.425506325734094
130-134	16.47588466244564	42.6761488828677	23.007144167299355	17.840822287387308
135-139	21.126164090633644	30.417644723369573	26.31628438290136	22.139906803095418
140-144	20.452727065876328	26.941590569792034	31.32163612343598	21.284046240895655
145-149	21.015135790258206	37.668777179528256	29.29879429116719	12.017292739046345
150-151	39.501475501566055	31.49406432768905	28.531336124132515	0.47312404661238305
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	2.0
27	2.5
28	0.5
29	0.5
30	0.5
31	1.5
32	3.5
33	4.5
34	8.5
35	40.5
36	371.5
37	405.5
38	138.0
39	242.5
40	1128.0
41	7209.5
42	69453.0
43	77232.5
44	17981.5
45	5781.5
46	9556.5
47	79161.5
48	81987.5
49	20527.5
50	107093.5
51	106760.5
52	10600.5
53	1206.5
54	440.5
55	663.0
56	1960.5
57	13692.0
58	121232.0
59	116678.0
60	8502.0
61	1012.5
62	206.0
63	56.5
64	25.0
65	19.0
66	7.5
67	0.5
68	1.0
69	2.0
70	1.0
71	0.5
72	0.5
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.157413147403884
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	861402.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	1.379313027847585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.31210546162383	1.0112034216215382
2	9.614450360930793	0.26522673276851516
3	3.5302714455240873	0.14608048189949097
4	1.8370504029398174	0.10135470214350183
5	1.291555644553731	0.0890729763361523
6	0.8491389591079097	0.07027370572502865
7	0.7365363245980824	0.07111399036007789
8	0.6003343857061457	0.0662439231415491
9	0.41410167623922983	0.05140582532012632
>10	4.32544622838431	1.3290841343538857
>50	1.2756425560997586	1.3058829810353425
>100	1.7740724290129093	4.865920175341739
>500	0.211199098692013	1.9630775927101292
>1k	0.16895927895361043	5.505053587582201
>5k	0.025343891843041564	1.9290526234532077
>10k+	0.03379185579072208	81.22995314620752
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TACACGCATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC	220999	25.65573332776102	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	196619	22.82546360468167	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	139932	16.244680184164885	No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	139560	16.20149477247557	No Hit
NACACGCATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC	5876	0.6821437609849988	No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC	5402	0.627117188026032	No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	5277	0.6126059609798908	No Hit
TACACGCATGGTAACAGCATTCGTCGACGAAGCCTGGCCCATTGTCAACC	4545	0.5276282153976889	No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG	4102	0.4762004267461649	No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	3990	0.4631983673128226	No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	3923	0.45542034961609096	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG	3380	0.3923835793276542	No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	3193	0.3706747836666272	No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCCTCCGCGGCTATATTGA	3121	0.36231631688804994	No Hit
TACACACATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC	2747	0.31889872556599586	No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	2026	0.23519796796385425	No Hit
TCCACGCATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC	1865	0.2165075075284246	No Hit
AGTTTAGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	1786	0.20733641203526343	No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA	1783	0.20698814258615605	No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	1761	0.20443416662603522	No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC	1655	0.19212864609090763	No Hit
TACACGCATGCTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC	1546	0.1794748561066726	No Hit
AGTTTGTAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA	1412	0.1639188207132094	No Hit
TACACGCATGATAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC	1221	0.14174566578670586	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA	1091	0.12665398965871916	No Hit
TACACGCATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACA	1057	0.12270693590216879	No Hit
TACACTCATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC	1040	0.12073340902389361	No Hit
TTCCATATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	962	0.11167840334710159	No Hit
TCCCGCAGATCATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	894	0.10378429583400084	No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA	885	0.10273948748667869	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10-11	0.0	0.0	1.1608981636912847E-4
12-13	0.0	0.0	1.1608981636912847E-4
14-15	0.0	0.0	1.1608981636912847E-4
16-17	0.0	0.0	1.741347245536927E-4
18-19	0.0	0.0	2.3217963273825695E-4
20-21	0.0	0.0	0.0028442005010436474
22-23	0.0	0.0	0.005688401002087295
24-25	0.0	0.0	0.006268850083932937
26-27	0.0	0.0	0.00644298480848663
28-29	0.0	0.0	0.011725071453281975
30-31	0.0	0.0	0.011841161269651103
32-33	0.0	0.0	0.015265810852540394
34-35	0.0	0.0	0.018690460435429684
36-37	0.0	0.0	0.02205706511013441
38-39	0.0	0.0	0.028616139734990165
40-41	0.0	0.0	0.033375822206124436
42-43	0.0	0.0	0.04980253122235612
44-45	0.0	0.0	0.060366704511946805
46-47	0.0	0.0	0.14627316862510187
48-49	0.0	0.0	0.2935911455975259
50-51	0.0	0.0	0.34176841939071423
52-53	0.0	0.0	0.34223277865619073
54-55	0.0	0.0	0.34234886847255985
56-57	0.0	0.0	0.34234886847255985
58-59	0.0	0.0	0.3424069133807444
60-61	0.0	0.0	0.3427551828298518
62-63	0.0	0.0	0.3450189342490498
64-65	0.0	0.0	0.3450189342490498
66-67	0.0	0.0	0.34507697915723434
68-69	0.0	0.0	0.3461798324127411
70-71	0.0	0.0	0.34641201204547933
72-73	0.0	0.0	0.34641201204547933
74-75	0.0	0.0	0.3464700569536639
76-77	0.0	0.0	0.3500107963529223
78-79	0.0	0.0	0.3521004130475666
80-81	0.0	0.0	0.3522165028639358
82-83	0.0	0.0	0.3523325926803049
84-85	0.0	0.0	0.3523325926803049
86-87	0.0	0.0	0.3523906375884895
88-89	0.0	0.0	0.3535515357521808
90-91	0.0	0.0	0.35453829919131835
92-93	0.0	0.0	0.3545963440995029
94-95	0.0	0.0	0.3547704788240566
96-97	0.0	0.0	0.3548865686404257
98-99	0.0	0.0	0.3548865686404257
100-101	0.0	0.0	0.3548865686404257
102-103	0.0	0.0	0.355118748273164
104-105	0.0	0.0	0.3552348380895331
106-107	0.0	0.0	0.3552348380895331
108-109	0.0	0.0	0.3553509279059022
110-111	0.0	0.0	0.3555831075386405
112-113	0.0	0.0	0.3555831075386405
114-115	0.0	0.0	0.35564115244682504
116-117	0.0	0.0	0.3559894218959324
118-119	0.0	0.0	0.35761467932510027
120-121	0.0	0.0	0.35900775712152977
122-123	0.0	0.0	0.35918189184608346
124-125	0.0	0.0	0.3597042960197446
126-127	0.0	0.0	0.36069105945888214
128-129	0.0	0.0	0.36069105945888214
130-131	0.0	0.0	0.36080714927525126
132-133	0.0	0.0	0.3609232390916204
134-135	0.0	0.0	0.3609232390916204
136-137	0.0	0.0	0.3609232390916204
138-139	0.0	0.0	0.36115541872435863
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTGT	65	0.0	148.16925	1
CGTTTGG	520	0.0	148.16925	1
TGCACGC	15	1.1284344E-4	148.16925	1
TATACGC	15	1.1284344E-4	148.16925	1
TTCCGCA	55	0.0	148.16925	1
ACCCGCA	35	9.094947E-12	148.16925	1
TACACTC	140	0.0	148.16925	1
TACACCC	85	0.0	148.16925	1
AGTGTGT	15	1.1284344E-4	148.16925	1
AGTTGGA	60	0.0	148.16925	1
CGTGTGG	65	0.0	148.16925	1
TCCCGTA	70	0.0	148.16925	1
TCCCGAA	265	0.0	148.16925	1
AGTTTTG	55	0.0	148.16925	1
AGTTTCG	60	0.0	148.16925	1
AGTTAGG	35	9.094947E-12	148.16925	1
AGCTTGG	25	3.261448E-8	148.16924	1
TTCCGGA	20	1.9130512E-6	148.16924	1
AGTGGAA	10	0.0066468036	148.16924	1
TTTCAGA	25	3.261448E-8	148.16924	1
>>END_MODULE