FastQCFastQC Report
Wed 17 Jun 2015
000000000-AG1W8 l01n01 mis13.3410000000d22f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG1W8 l01n01 mis13.3410000000d22f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences861402
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACACGCATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC22099925.65573332776102No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC19661922.82546360468167No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG13993216.244680184164885No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA13956016.20149477247557No Hit
NACACGCATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC58760.6821437609849988No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC54020.627117188026032No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC52770.6126059609798908No Hit
TACACGCATGGTAACAGCATTCGTCGACGAAGCCTGGCCCATTGTCAACC45450.5276282153976889No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG41020.4762004267461649No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG39900.4631983673128226No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG39230.45542034961609096No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG33800.3923835793276542No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA31930.3706747836666272No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCCTCCGCGGCTATATTGA31210.36231631688804994No Hit
TACACACATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC27470.31889872556599586No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG20260.23519796796385425No Hit
TCCACGCATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC18650.2165075075284246No Hit
AGTTTAGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA17860.20733641203526343No Hit
AGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA17830.20698814258615605No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC17610.20443416662603522No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC16550.19212864609090763No Hit
TACACGCATGCTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC15460.1794748561066726No Hit
AGTTTGTAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGGCTATATTGA14120.1639188207132094No Hit
TACACGCATGATAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC12210.14174566578670586No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA10910.12665398965871916No Hit
TACACGCATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACA10570.12270693590216879No Hit
TACACTCATGGTAACAGCATTCGTCGACGAGGCCTGGCCCATTGTCAACC10400.12073340902389361No Hit
TTCCATATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC9620.11167840334710159No Hit
TCCCGCAGATCATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG8940.10378429583400084No Hit
CGTTTGGAAACTCCGATCTTTTTCCGTTGGTCCATCCGCGTCTATATTGA8850.10273948748667869No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTGT650.0148.169251
CGTTTGG5200.0148.169251
TGCACGC151.1284344E-4148.169251
TATACGC151.1284344E-4148.169251
TTCCGCA550.0148.169251
ACCCGCA359.094947E-12148.169251
TACACTC1400.0148.169251
TACACCC850.0148.169251
AGTGTGT151.1284344E-4148.169251
AGTTGGA600.0148.169251
CGTGTGG650.0148.169251
TCCCGTA700.0148.169251
TCCCGAA2650.0148.169251
AGTTTTG550.0148.169251
AGTTTCG600.0148.169251
AGTTAGG359.094947E-12148.169251
AGCTTGG253.261448E-8148.169241
TTCCGGA201.9130512E-6148.169241
AGTGGAA100.0066468036148.169241
TTTCAGA253.261448E-8148.169241
Produced by FastQC (version 0.11.2)