##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AG1W8 l01n01 mis12.3410000000d212.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	848839
Sequences flagged as poor quality	0
Sequence length	151
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.027846270022934	34.0	34.0	34.0	34.0	34.0
2	33.522332267956585	34.0	34.0	34.0	34.0	34.0
3	33.850968204806804	34.0	34.0	34.0	34.0	34.0
4	33.758862399112196	34.0	34.0	34.0	34.0	34.0
5	33.789291019851824	34.0	34.0	34.0	34.0	34.0
6	36.40489303625305	38.0	38.0	38.0	34.0	38.0
7	37.11488751105922	38.0	38.0	38.0	36.0	38.0
8	37.211640841196036	38.0	38.0	38.0	36.0	38.0
9	37.42172779525917	38.0	38.0	38.0	37.0	38.0
10-14	37.27646585512683	38.0	38.0	38.0	36.4	38.0
15-19	37.22723461103932	38.0	38.0	38.0	36.2	38.0
20-24	37.45405477363788	38.0	38.0	38.0	36.6	38.0
25-29	37.15014154627674	38.0	38.0	38.0	36.0	38.0
30-34	37.010553944858806	38.0	38.0	38.0	35.8	38.0
35-39	37.0319367983799	38.0	38.0	38.0	35.8	38.0
40-44	37.02695187190975	38.0	38.0	38.0	36.2	38.0
45-49	36.789587895937856	38.0	37.8	38.0	34.8	38.0
50-54	36.98905328336705	38.0	38.0	38.0	36.0	38.0
55-59	36.60066608626606	38.0	37.6	38.0	34.6	38.0
60-64	36.58561941663849	38.0	37.2	38.0	34.2	38.0
65-69	36.64255200338345	38.0	37.4	38.0	34.6	38.0
70-74	36.585506085370724	38.0	37.4	38.0	34.0	38.0
75-79	36.32233698027541	38.0	37.0	38.0	33.4	38.0
80-84	36.332211644375434	38.0	37.0	38.0	33.8	38.0
85-89	35.98418993472261	38.0	37.0	38.0	32.0	38.0
90-94	36.22698038143865	38.0	37.0	38.0	33.6	38.0
95-99	35.90341301471774	38.0	36.8	38.0	32.0	38.0
100-104	35.77539769025692	38.0	36.8	38.0	31.4	38.0
105-109	35.9969087188501	38.0	37.0	38.0	32.4	38.0
110-114	35.852253960998496	38.0	37.0	38.0	31.2	38.0
115-119	35.26611642490508	38.0	35.6	38.0	28.2	38.0
120-124	35.390040278545165	38.0	36.4	38.0	28.8	38.0
125-129	35.60461123958725	38.0	37.0	38.0	30.2	38.0
130-134	35.41337874437909	38.0	36.4	38.0	29.4	38.0
135-139	35.376955111628945	38.0	36.4	38.0	29.4	38.0
140-144	35.45737130362766	38.0	36.8	38.0	29.6	38.0
145-149	35.01016400047594	37.8	35.4	38.0	27.4	38.0
150-151	32.988454818876136	36.0	32.5	37.5	22.5	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.8445033940241871
1101	2	0.3907945014388048
1101	3	0.06952527728370228
1101	4	0.03244585822595525
1101	5	0.13224006204305283
1101	6	1.2773197570177501
1101	7	0.47601597932363404
1101	8	0.4026591976544438
1101	9	0.17177133007592715
1101	10-14	0.27165035026217055
1101	15-19	0.3195630906176561
1101	20-24	0.14836629824446135
1101	25-29	0.3263924341883353
1101	30-34	0.23838526291073237
1101	35-39	0.27531596706723604
1101	40-44	0.30304180009210313
1101	45-49	0.40436174891496535
1101	50-54	0.2941654893066783
1101	55-59	0.42161159094266765
1101	60-64	0.4879527347434305
1101	65-69	0.39688411546340063
1101	70-74	0.3410188268350751
1101	75-79	0.4649590699525845
1101	80-84	0.4891378475353392
1101	85-89	0.676738235185546
1101	90-94	0.5382916409582776
1101	95-99	0.5939010054605802
1101	100-104	0.7058134254906321
1101	105-109	0.5104457088075236
1101	110-114	0.5422657492080774
1101	115-119	0.7582000334871637
1101	120-124	0.6822219051788707
1101	125-129	0.5580166427328876
1101	130-134	0.7361176313174411
1101	135-139	0.7586409217020815
1101	140-144	0.6820841292260624
1101	145-149	0.8446337376973077
1101	150-151	0.7193370199197986
1102	1	0.9071085908766534
1102	2	0.3821743916758038
1102	3	0.09119564609966346
1102	4	0.11921580486528427
1102	5	0.11710268281379399
1102	6	1.1425405625605904
1102	7	0.48801262472305496
1102	8	0.159166230763212
1102	9	0.20959101517632917
1102	10-14	0.2045046708093281
1102	15-19	0.21284255633829474
1102	20-24	0.12331069288821794
1102	25-29	0.2424353654716782
1102	30-34	0.1547897569641421
1102	35-39	0.23633255435338896
1102	40-44	0.2928289862197886
1102	45-49	0.28103930511622366
1102	50-54	0.15923274948709576
1102	55-59	0.3270267702909635
1102	60-64	0.43460837051627976
1102	65-69	0.39587407944604536
1102	70-74	0.2771936755197757
1102	75-79	0.3416699674325443
1102	80-84	0.49062065703526514
1102	85-89	0.5608708574332368
1102	90-94	0.4458338622771052
1102	95-99	0.515742760147738
1102	100-104	0.7919770872989247
1102	105-109	0.31916840149598613
1102	110-114	0.3453138440025967
1102	115-119	0.684104744697585
1102	120-124	0.6483726838000905
1102	125-129	0.6077439169224945
1102	130-134	0.6874125305404988
1102	135-139	0.741220396050501
1102	140-144	0.5729236860210278
1102	145-149	0.7205946026402756
1102	150-151	1.0255543641114642
1103	1	0.8949179589824894
1103	2	0.3773786932629406
1103	3	0.06584445742100797
1103	4	0.06725044248974399
1103	5	0.13271296937304555
1103	6	1.2482348547311233
1103	7	0.5243479896424148
1103	8	0.30549546670933836
1103	9	0.17041319015851286
1103	10-14	0.19758146000090449
1103	15-19	0.25812919740805995
1103	20-24	0.13161076563257268
1103	25-29	0.29054326812810416
1103	30-34	0.2645299152761069
1103	35-39	0.2566484642455862
1103	40-44	0.27176287215245765
1103	45-49	0.2993191909269086
1103	50-54	0.22857419726363304
1103	55-59	0.40515228630737
1103	60-64	0.4884584665418217
1103	65-69	0.35392973706851905
1103	70-74	0.24417085699540308
1103	75-79	0.3268583069122073
1103	80-84	0.4265599444974981
1103	85-89	0.5824565953720295
1103	90-94	0.45000476244773324
1103	95-99	0.4715790451568864
1103	100-104	0.59970242004119
1103	105-109	0.4762316712437027
1103	110-114	0.4659671449455871
1103	115-119	0.8404832653241172
1103	120-124	0.7297784649689731
1103	125-129	0.6687433412155386
1103	130-134	0.6909775251776509
1103	135-139	0.7389144442521953
1103	140-144	0.8124852412926131
1103	145-149	0.9307951033714374
1103	150-151	1.019768658007635
2116	1	0.7329317194872047
2116	2	0.35195984752415654
2116	3	-0.041148593963583835
2116	4	-0.12236179254241364
2116	5	0.0831961496219833
2116	6	1.13203476585916
2116	7	0.06384229841876277
2116	8	0.2735918976699878
2116	9	0.1894205587183464
2116	10-14	0.2222331746926116
2116	15-19	0.2823360639878132
2116	20-24	0.1460636903502177
2116	25-29	0.2876417450970834
2116	30-34	0.2721035642514025
2116	35-39	0.1546312660562208
2116	40-44	0.08686719904500961
2116	45-49	0.27852266376968515
2116	50-54	0.20327840500916494
2116	55-59	0.40017740972707827
2116	60-64	0.5178247899283264
2116	65-69	0.3987353662771085
2116	70-74	0.3230632779750451
2116	75-79	0.3209004673798077
2116	80-84	0.4690544144153179
2116	85-89	0.5017242318173487
2116	90-94	0.5178453202901991
2116	95-99	0.6369995511410949
2116	100-104	0.7038657295939359
2116	105-109	0.40402808609020013
2116	110-114	0.4586215355622727
2116	115-119	0.8190451021415157
2116	120-124	0.5615399365323839
2116	125-129	0.48418508857502474
2116	130-134	0.5866535624648108
2116	135-139	0.6679423705568368
2116	140-144	0.6411264280751041
2116	145-149	0.7876824361871684
2116	150-151	1.6527848356343569
1117	1	0.909646252882581
1117	2	0.3055032446951529
1117	3	0.054987208107412755
1117	4	-0.024550578183315963
1117	5	0.08297424579464519
1117	6	1.1272841811540957
1117	7	0.3561575922762543
1117	8	0.25441142152119767
1117	9	0.11376728613598885
1117	10-14	0.11699501583404981
1117	15-19	0.1529307739234227
1117	20-24	0.07580106832230626
1117	25-29	0.2442596236350667
1117	30-34	0.16690652286672503
1117	35-39	0.13000132332123115
1117	40-44	0.22839350578212958
1117	45-49	0.2915507603262597
1117	50-54	0.14886908381988917
1117	55-59	0.3306904913032298
1117	60-64	0.42738872971177244
1117	65-69	0.3655051529576596
1117	70-74	0.24181875838531397
1117	75-79	0.211526374382899
1117	80-84	0.3816900329741557
1117	85-89	0.4287579535207726
1117	90-94	0.34800205042596133
1117	95-99	-0.2048265945045955
1117	100-104	0.4215965748402084
1117	105-109	0.4494097606732268
1117	110-114	0.5281541400682244
1117	115-119	0.8451292059955833
1117	120-124	0.7545731545371552
1117	125-129	0.5479895030022988
1117	130-134	0.5974374358153156
1117	135-139	0.3519907870541914
1117	140-144	0.47595930147628707
1117	145-149	-0.6074867789182719
1117	150-151	0.06184908869379058
2117	1	-30.971979188813535
2117	2	-10.519260626834502
2117	3	-1.815853218938365
2117	4	-0.3363001662765086
2117	5	0.0753336102665898
2117	6	1.3044349690490904
2117	7	0.09155697471607738
2117	8	0.33763974219299797
2117	9	0.3332242463751953
2117	10-14	0.4216351181549527
2117	15-19	0.4647316682026741
2117	20-24	0.30754528148172966
2117	25-29	0.42532734094269387
2117	30-34	0.5330859736462159
2117	35-39	0.42268369816757456
2117	40-44	0.2702934291443313
2117	45-49	0.5795356402174932
2117	50-54	0.4939710789747309
2117	55-59	0.6691414131000926
2117	60-64	0.7116097843646472
2117	65-69	0.5739249395719384
2117	70-74	0.6283779092315029
2117	75-79	0.6224215451720738
2117	80-84	0.669126403873733
2117	85-89	0.7465521561360546
2117	90-94	0.757689018397528
2117	95-99	0.1062016637596841
2117	100-104	0.9100216506636372
2117	105-109	0.7676535460667822
2117	110-114	0.906654319232679
2117	115-119	1.1502548097112282
2117	120-124	0.8953155811954545
2117	125-129	0.8453737876217602
2117	130-134	1.1014538486513743
2117	135-139	1.0004750900187176
2117	140-144	0.8968562639100455
2117	145-149	1.1156766567874143
2117	150-151	1.5519698618270112
1116	1	0.35624443607375156
1116	2	0.22454102425015776
1116	3	-0.10809384731130933
1116	4	-0.28891187524844497
1116	5	0.0849545100635396
1116	6	1.213826349790402
1116	7	-0.2854551999441597
1116	8	0.2341085350828962
1116	9	0.12989337623721298
1116	10-14	0.24042203600629364
1116	15-19	0.23401877221558465
1116	20-24	0.08532489681198285
1116	25-29	0.04999617679631996
1116	30-34	0.2031714067113981
1116	35-39	0.16125444984466952
1116	40-44	0.05869099149970225
1116	45-49	0.24651105945290652
1116	50-54	0.17268395221116606
1116	55-59	0.3225785768990761
1116	60-64	0.43614881026056906
1116	65-69	0.2640491887458012
1116	70-74	0.306230239138678
1116	75-79	0.3196562911895029
1116	80-84	0.34920190978036914
1116	85-89	0.441711080362829
1116	90-94	0.4442583472839843
1116	95-99	-0.5445571797897841
1116	100-104	0.5846597075131399
1116	105-109	0.4738982056798733
1116	110-114	0.5635250373525693
1116	115-119	0.9563116452095883
1116	120-124	0.7006871846218772
1116	125-129	0.41157542225595023
1116	130-134	0.5588298499534048
1116	135-139	0.7834921236927457
1116	140-144	0.5824555400893772
1116	145-149	0.6609742068486852
1116	150-151	0.670814006312888
2118	1	0.8501466246354923
2118	2	0.3621810000740737
2118	3	0.021235109811343023
2118	4	-0.012398473755240502
2118	5	0.0651860606386947
2118	6	1.2317360180733772
2118	7	0.5003996379137163
2118	8	0.23025131482427952
2118	9	0.033908054016535516
2118	10-14	0.22293478419748425
2118	15-19	0.1854877824239125
2118	20-24	0.09755455398490653
2118	25-29	0.24558122439543695
2118	30-34	0.2534984684758257
2118	35-39	0.09540454109178853
2118	40-44	0.20908960352632988
2118	45-49	0.297754856506792
2118	50-54	0.07566077954971462
2118	55-59	0.2498235788452945
2118	60-64	0.356169035239013
2118	65-69	0.3121636691653151
2118	70-74	0.2505176998476415
2118	75-79	0.3487845242031611
2118	80-84	0.30168819337212227
2118	85-89	0.24102871227995593
2118	90-94	0.35916912461558326
2118	95-99	0.4578728298532653
2118	100-104	0.6409077588540697
2118	105-109	0.3300856994451351
2118	110-114	0.5363786175062799
2118	115-119	0.9522148548002249
2118	120-124	0.5850011686101126
2118	125-129	0.42844242835277413
2118	130-134	0.5633404504631514
2118	135-139	0.5424532170151792
2118	140-144	0.49651428641454487
2118	145-149	0.5247106640349131
2118	150-151	1.4048222879916779
1119	1	0.8110146110093197
1119	2	0.3182940442707505
1119	3	0.022828288271540487
1119	4	-0.02880713594261408
1119	5	0.08619326760010892
1119	6	1.2576005748481336
1119	7	0.27416330107136844
1119	8	0.3353392259198458
1119	9	0.15727063661031337
1119	10-14	0.21758968199158346
1119	15-19	0.18254835663678648
1119	20-24	0.08651796967345149
1119	25-29	0.22971753222548585
1119	30-34	0.17868505980018767
1119	35-39	0.15733906553032284
1119	40-44	0.02332937573178384
1119	45-49	0.21098515456489508
1119	50-54	0.2296536206219031
1119	55-59	0.2872588919955419
1119	60-64	0.36506904657493777
1119	65-69	0.27818108807019115
1119	70-74	0.2024648475163886
1119	75-79	0.37013338455795974
1119	80-84	0.24566014943482628
1119	85-89	0.38607281673191096
1119	90-94	0.35987268236829806
1119	95-99	0.4697879679306496
1119	100-104	0.6796052413169988
1119	105-109	0.3945506339561362
1119	110-114	0.36746861518144414
1119	115-119	0.848903379957342
1119	120-124	0.7735018478052211
1119	125-129	0.4292176138105148
1119	130-134	0.7584515137541459
1119	135-139	0.5974217165799942
1119	140-144	0.5756517182308514
1119	145-149	0.6686664039540915
1119	150-151	1.2568379700015555
2119	1	0.9067691814602625
2119	2	0.37890465412998253
2119	3	0.09270625175566494
2119	4	0.06147132349409645
2119	5	0.09797233388969318
2119	6	1.251548020965636
2119	7	0.4049983175408869
2119	8	0.23534765603591268
2119	9	0.14378822495041987
2119	10-14	0.15313278355831983
2119	15-19	0.12953293491349882
2119	20-24	0.10364734962878686
2119	25-29	0.13614239698756592
2119	30-34	0.05941342952737472
2119	35-39	0.1076082325925114
2119	40-44	-0.21926057148953504
2119	45-49	0.1470146237870722
2119	50-54	0.1047125640704536
2119	55-59	0.17575324631987144
2119	60-64	0.20091909675145558
2119	65-69	0.22368104207024686
2119	70-74	0.09385858436356642
2119	75-79	0.10716730788393392
2119	80-84	0.19699878642747848
2119	85-89	0.299551263542682
2119	90-94	0.24891876661787649
2119	95-99	0.2374341447960191
2119	100-104	0.6039091889594417
2119	105-109	0.2912944920006808
2119	110-114	0.42220258727892457
2119	115-119	0.8330390178303517
2119	120-124	0.41821094913559875
2119	125-129	0.5498087304698558
2119	130-134	0.47956968588940896
2119	135-139	0.29706014543732095
2119	140-144	0.24164794046102855
2119	145-149	0.33970329668260035
2119	150-151	0.3776870520620008
1118	1	0.8724938188985476
1118	2	0.3736470763919044
1118	3	0.10904596769452723
1118	4	0.10732542291884783
1118	5	0.11362273290029634
1118	6	1.2446928367325611
1118	7	0.4348506562468728
1118	8	0.39155446898971036
1118	9	0.06205780484694401
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2104	145-149	-0.6505368884856892
2104	150-151	-0.05273088055486852
2111	1	0.9012026293682851
2111	2	0.30144445895792416
2111	3	0.09655161543153667
2111	4	0.04854611311125723
2111	5	0.048973282094031845
2111	6	0.9880631724807927
2111	7	0.36000918238225665
2111	8	0.008126487374070734
2111	9	-0.08011482115601609
2111	10-14	-0.16600980266900223
2111	15-19	-0.04312255084368388
2111	20-24	-0.1270534128855303
2111	25-29	-0.07073330922712984
2111	30-34	-0.09790105824474438
2111	35-39	-0.22858717651814686
2111	40-44	-0.21892676405403222
2111	45-49	-0.3238353623849548
2111	50-54	-0.37946917509434286
2111	55-59	-0.4130358177234399
2111	60-64	-0.24021294070262655
2111	65-69	-0.5445687307557989
2111	70-74	-0.36278066339432513
2111	75-79	-0.605184515433983
2111	80-84	-0.2970940980891328
2111	85-89	-0.6231598106938137
2111	90-94	-0.8598578917520072
2111	95-99	-0.5379554091528433
2111	100-104	-0.4843012728950882
2111	105-109	-0.3910903999435362
2111	110-114	-0.7928599942073831
2111	115-119	-2.342612201007306
2111	120-124	-1.3542887150420313
2111	125-129	-0.9487046826706802
2111	130-134	-0.5556181375865563
2111	135-139	-0.5698187214584962
2111	140-144	-1.123529294733153
2111	145-149	-1.4388921756416835
2111	150-151	-2.721087788916229
2110	1	0.9563669130341452
2110	2	0.2918088737229638
2110	3	0.06011956168087096
2110	4	-0.016477034088545395
2110	5	0.07013699220095759
2110	6	1.1189056895851337
2110	7	0.14949994398658362
2110	8	-0.08334776392668175
2110	9	-0.2074453140070105
2110	10-14	-0.10338773703747961
2110	15-19	-0.16411575007347068
2110	20-24	-0.20117472059989439
2110	25-29	-0.16911140746957187
2110	30-34	-0.12409414276275044
2110	35-39	-0.3851114913200675
2110	40-44	-0.31367996983878044
2110	45-49	-0.23741658242673225
2110	50-54	-0.2856121551426867
2110	55-59	-0.46124918164657913
2110	60-64	-0.19432548115900516
2110	65-69	-0.35663682039486844
2110	70-74	-0.34815802420385467
2110	75-79	-0.5761664073590964
2110	80-84	-0.46624420665563804
2110	85-89	-0.43409594080975467
2110	90-94	-0.5697405579816675
2110	95-99	-0.7939218559552543
2110	100-104	-0.23533142570758514
2110	105-109	-0.4222167877513101
2110	110-114	-0.8489118193538587
2110	115-119	-6.514169741258851
2110	120-124	-3.274017462635129
2110	125-129	-0.6215021344302869
2110	130-134	-0.8541293015015015
2110	135-139	-0.9406758233471422
2110	140-144	-0.7886233583981976
2110	145-149	-0.7476155973304088
2110	150-151	-1.3177648342683526
2109	1	0.9797072286887456
2109	2	0.3843329150779198
2109	3	0.10458923870161385
2109	4	0.1364345692312554
2109	5	0.09230261001314943
2109	6	1.1035686833782776
2109	7	0.4401732662473492
2109	8	0.011890057441192425
2109	9	-0.09740412255652586
2109	10-14	-0.010018586956732634
2109	15-19	-0.04006868492837867
2109	20-24	-0.3056397226559753
2109	25-29	-0.015184539856115009
2109	30-34	-0.14830803595253883
2109	35-39	-0.4405392772888419
2109	40-44	-0.15808753309356405
2109	45-49	-0.37880776646218806
2109	50-54	-0.1883837354655995
2109	55-59	-0.48419849278765525
2109	60-64	-0.4058115981982766
2109	65-69	-0.5954521317668195
2109	70-74	-0.4169687797446997
2109	75-79	-0.3766364918987293
2109	80-84	-0.397380416146639
2109	85-89	-0.6303132042464767
2109	90-94	-0.682849356076197
2109	95-99	-0.3364600084733027
2109	100-104	-0.3890650915741318
2109	105-109	-0.4307963749166106
2109	110-114	-1.2159019267506963
2109	115-119	-4.8521857486993625
2109	120-124	-1.5963635472289894
2109	125-129	-0.9904399109792976
2109	130-134	-1.0372719869277276
2109	135-139	-1.218381993191322
2109	140-144	-0.7232063478532353
2109	145-149	-0.7798377296319075
2109	150-151	-1.9029076281471902
2108	1	0.846968179607515
2108	2	0.11400426757035831
2108	3	0.018513337919578987
2108	4	-0.04442039406577436
2108	5	-0.12038078489161563
2108	6	0.8275129332463393
2108	7	-0.07788555445984713
2108	8	-0.5108926513819085
2108	9	-0.4757129072804176
2108	10-14	-0.23472750601828807
2108	15-19	-0.3876440819441669
2108	20-24	-0.4879577191056228
2108	25-29	-0.2546806776481816
2108	30-34	-4.4475039290581435
2108	35-39	-0.9185584683841768
2108	40-44	-0.5589985343889623
2108	45-49	-0.28713679779164636
2108	50-54	-0.3600624765297482
2108	55-59	-0.4422702674841972
2108	60-64	-0.6259880603198482
2108	65-69	-0.5585208838658531
2108	70-74	-0.5749050653081937
2108	75-79	-0.8220696828981033
2108	80-84	-0.7261515143570705
2108	85-89	-1.1676478489713276
2108	90-94	-1.1040492793118162
2108	95-99	-1.0056156962341802
2108	100-104	-0.8495596461008361
2108	105-109	-0.8515880075990339
2108	110-114	-1.3310178889442383
2108	115-119	-1.3745165072274048
2108	120-124	-1.1585040260468062
2108	125-129	-0.8955228644888678
2108	130-134	-1.3199482811272176
2108	135-139	-1.872761305190565
2108	140-144	-1.5477254669819587
2108	145-149	-1.6423275823402506
2108	150-151	-2.158527980303788
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	11.0
18	29.0
19	80.0
20	199.0
21	535.0
22	1223.0
23	1966.0
24	2494.0
25	3150.0
26	3971.0
27	5360.0
28	7076.0
29	9440.0
30	12401.0
31	16284.0
32	22009.0
33	29478.0
34	41720.0
35	65970.0
36	132316.0
37	493126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.10902794882568569	0.07992968119889693	99.6459912004907	0.16505116948473125
2	53.525698041678105	0.03993690205091896	27.26217810444619	19.172186951824788
3	0.0652656157410298	0.10461347793869037	0.09825184752349976	99.73186905879679
4	0.32962670188339604	26.541664555940525	0.16175034370475438	72.96695839847132
5	72.30157898023064	27.34299437231324	0.17612291612425912	0.17930373133185445
6	28.288521144763614	27.481065313917007	25.62264457688678	18.607768964432594
7	0.6239110125712886	45.862171742815775	26.537894700879672	26.976022543733265
8	72.3124173135306	0.28073639406294953	27.06496756157528	0.34187873083117054
9	53.28407389387151	19.03399820225037	0.6644369544754659	27.017490949402656
10-14	18.45560818953889	41.70600078460108	23.738258963124927	16.1001320627351
15-19	30.832631394174868	22.19490386280555	19.945596279153055	27.026868463866528
20-24	34.67722383161	23.97429901312263	10.696256887348484	30.65222026791889
25-29	23.827463158502376	44.872655474124066	5.573023859648297	25.72685750772526
30-34	23.637038354741005	16.278728946243046	36.05315024403921	24.031082454976737
35-39	43.26224407690976	20.278238865085136	20.214622560933226	16.244894497071883
40-44	14.526358944393461	30.775023296526193	27.184731144539775	27.513886614540567
45-49	16.34698688443863	26.940727275726022	22.11415827972089	34.59812756011446
50-54	30.552389793588652	25.61663637038355	16.31925488814722	27.511718947880574
55-59	21.146507170382133	43.60094199253333	29.609643289245664	5.642907547838872
60-64	37.894912934019295	20.116629890945163	20.261203832528903	21.727253342506646
65-69	19.93200123933985	34.5681807739748	25.44491947236166	20.054898514323682
70-74	25.2729905199926	14.54537315085664	29.433826673845097	30.74780965530566
75-79	29.268023735949928	16.5023049129458	28.701343835521225	25.52832751558305
80-84	26.72158088871977	20.055157691859115	31.09668617959354	22.126575239827577
85-89	30.504135648809726	20.2976300570544	38.13083517604634	11.067399118089531
90-94	26.623399725978658	34.706251715578574	29.122248153065538	9.548100405377227
95-99	16.171547254544148	30.82850811520206	31.630992449687163	21.368952180566634
100-104	9.357628478427593	39.494203258804085	30.690107311280467	20.458060951487855
105-109	20.0390651230681	27.547721063711727	30.924898596789262	21.488315216430916
110-114	41.01680059469464	26.96798804013482	11.744842072524943	20.2703692926456
115-119	9.32504279374534	40.28933637592052	16.05583626577007	34.32978456456407
120-124	5.668707493411589	20.293813078805286	47.148988206244056	26.88849122153907
125-129	19.837448562094814	29.111692558895147	20.050751673756743	31.00010720525329
130-134	16.017053881831536	31.400795675033784	46.878595352004325	5.703555091130355
135-139	30.475343380782455	0.56785798013522	48.78767351641478	20.16912512266755
140-144	13.213530481045286	37.660592880393104	18.628126181761207	30.497750456800404
145-149	25.466478330990917	18.561753171096047	34.37676638325996	21.595002114653074
150-151	39.556853537596645	19.253886779471728	27.219001483202348	13.970258199729276
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.5
21	0.5
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	2.0
29	3.0
30	3.5
31	11.5
32	33.0
33	104.0
34	494.0
35	4429.5
36	101943.5
37	109176.5
38	12155.5
39	1137.5
40	345.5
41	230.5
42	258.5
43	354.5
44	611.0
45	1404.0
46	7624.0
47	71601.0
48	74662.5
49	18821.0
50	101026.0
51	103280.0
52	12602.0
53	1442.5
54	328.0
55	187.5
56	184.0
57	200.0
58	299.5
59	597.0
60	1659.0
61	8825.0
62	98194.5
63	101280.5
64	11723.0
65	1266.5
66	248.5
67	59.0
68	16.5
69	5.5
70	3.5
71	2.0
72	1.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.428140083101743
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	848839.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	1.2810249024700822
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.5503916032193	0.9421988323015033
2	9.30296050774756	0.23834648154240687
3	3.6604234051410987	0.14067280606710242
4	2.0844508989818684	0.1068093403828769
5	1.2569478568391506	0.08050907528586757
6	0.8541908285119986	0.06565438336712529
7	0.7235280161876754	0.0648800184459832
8	0.5285665904609006	0.05416855719952952
9	0.3987014721741056	0.045967186305586064
>10	4.107344350904847	1.1801197383057453
>50	1.5576353567760972	1.4492560028296562
>100	1.6318084690035304	3.957720744655778
>500	0.13907458542643727	1.1906893483336587
>1k	0.12980294639800813	5.036764408457403
>5k	0.037086556113716605	2.478177880596687
>10k+	0.037086556113716605	82.96806519592307
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	197444	23.26047695734998	No Hit
TGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	187992	22.146956018750316	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	185810	21.889899026788353	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	127303	14.997308087870609	No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	5231	0.6162534944789294	No Hit
TGCAGTTGGTCGACAGTCCTCGGTCCGCAACGGTGTGGACATGGCAGAGG	5218	0.6147219908604576	No Hit
NGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	5215	0.6143685669485026	No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC	5201	0.6127192553593791	No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	4917	0.5792617916943025	No Hit
TGCAGGTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	4800	0.565478259128056	No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC	4793	0.5646536033334943	No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG	4354	0.5129359042174075	No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG	3762	0.443193585591614	No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	3653	0.43035251679058095	No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	3561	0.4195141834906266	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG	3087	0.3636732054017311	No Hit
TGCAGATGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	2821	0.33233628520838465	No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	1566	0.1844872820405283	No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC	1526	0.17977496321446115	No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	1366	0.16092568791019263	No Hit
TGCAGCTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	1142	0.13453670248421667	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA	1059	0.12475864092012737	No Hit
TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	929	0.10944360473540918	No Hit
TTCCAGATCTCGATGCCGACAGGGAAAGCAATAGAATCCAAAGTACCTGT	864	0.10178608664305008	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	1.1780797065167836E-4
6	0.0	0.0	1.1780797065167836E-4
7	0.0	0.0	1.1780797065167836E-4
8	0.0	0.0	1.1780797065167836E-4
9	0.0	0.0	1.1780797065167836E-4
10-11	0.0	0.0	1.1780797065167836E-4
12-13	0.0	0.0	1.1780797065167836E-4
14-15	0.0	0.0	1.1780797065167836E-4
16-17	0.0	0.0	1.1780797065167836E-4
18-19	0.0	0.0	1.1780797065167836E-4
20-21	0.0	0.0	1.1780797065167836E-4
22-23	0.0	0.0	2.945199266291959E-4
24-25	0.0	0.0	4.712318826067134E-4
26-27	0.0	0.0	4.712318826067134E-4
28-29	0.0	0.0	4.712318826067134E-4
30-31	0.0	0.0	4.712318826067134E-4
32-33	0.0	0.0	5.301358679325526E-4
34-35	0.0	0.0	5.890398532583918E-4
36-37	0.0	0.0	0.0038287590461795465
38-39	0.0	0.0	0.0070095742537748616
40-41	0.0	0.0	0.008717789828224198
42-43	0.0	0.0	0.02933418469226791
44-45	0.0	0.0	0.04924373173240155
46-47	0.0	0.0	0.15456405749500202
48-49	0.0	0.0	0.31543084141986877
50-51	0.0	0.0	0.37174305139137104
52-53	0.0	0.0	0.3904745187249879
54-55	0.0	0.0	0.39118136654889796
56-57	0.0	0.0	0.3914758864755271
58-59	0.0	0.0	0.39206492632878553
60-61	0.0	0.0	0.39365533393258323
62-63	0.0	0.0	0.39583478138963923
64-65	0.0	0.0	0.39589368537496505
66-67	0.0	0.0	0.39618820530159427
68-69	0.0	0.0	0.39866217268527954
70-71	0.0	0.0	0.3989566926119087
72-73	0.0	0.0	0.39901559659723457
74-75	0.0	0.0	0.39901559659723457
76-77	0.0	0.0	0.39972244442114463
78-79	0.0	0.0	0.40019367630375136
80-81	0.0	0.0	0.4002525802890772
82-83	0.0	0.0	0.40031148427440305
84-85	0.0	0.0	0.40031148427440305
86-87	0.0	0.0	0.40031148427440305
88-89	0.0	0.0	0.40119504405429063
90-91	0.0	0.0	0.40620188280698694
92-93	0.0	0.0	0.41044296975044736
94-95	0.0	0.0	0.4105018737357732
96-97	0.0	0.0	0.41067858569175075
98-99	0.0	0.0	0.41067858569175075
100-101	0.0	0.0	0.4107374896770766
102-103	0.0	0.0	0.42287171065419943
104-105	0.0	0.0	0.43459360373404143
106-107	0.0	0.0	0.43459360373404143
108-109	0.0	0.0	0.4348292196753448
110-111	0.0	0.0	0.4353004515579515
112-113	0.0	0.0	0.4354182595286032
114-115	0.0	0.0	0.4354182595286032
116-117	0.0	0.0	0.4356538754699065
118-119	0.0	0.0	0.4366552432204458
120-121	0.0	0.0	0.43883469067750186
122-123	0.0	0.0	0.47941953656700503
124-125	0.0	0.0	0.5169413752195646
126-127	0.0	0.0	0.5170591831902163
128-129	0.0	0.0	0.5172358951461937
130-131	0.0	0.0	0.5174126071021713
132-133	0.0	0.0	0.5174126071021713
134-135	0.0	0.0	0.5174126071021713
136-137	0.0	0.0	0.5174126071021713
138-139	0.0	0.0	0.5174126071021713
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCCACA	35	9.094947E-12	148.58176	1
TGCCGAG	35	9.094947E-12	148.58176	1
TGCACTT	10	0.006591762	148.58174	1
TGCATTT	20	1.8867395E-6	148.58174	1
GTGCAGT	10	0.006591762	148.58174	1
TACAGTT	10	0.006591762	148.58174	1
TGCCGTT	15	1.1159983E-4	148.58174	1
CTCCAGA	15	1.1159983E-4	148.58174	1
GTCCCGC	15	1.1159983E-4	148.58174	1
TCCCGGA	315	0.0	148.58174	1
TCCCGCG	15	1.1159983E-4	148.58174	1
GCCCGCA	10	0.006591762	148.58174	1
TGAGTTG	10	0.006591762	148.58174	1
TCCCCAG	10	0.006591762	148.58174	1
TGCCGCG	25	3.2077878E-8	148.58173	1
TCCCGTA	50	0.0	148.58173	1
TCCCGCA	14840	0.0	148.3815	1
TGCAGTT	20830	0.0	148.33209	1
TTCCAGA	22055	0.0	148.2449	1
TGCAGGT	465	0.0	146.98409	1
>>END_MODULE