FastQCFastQC Report
Wed 17 Jun 2015
000000000-AG1W8 l01n01 mis12.3410000000d212.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG1W8 l01n01 mis12.3410000000d212.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences848839
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG19744423.26047695734998No Hit
TGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG18799222.146956018750316No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC18581021.889899026788353No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG12730314.997308087870609No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG52310.6162534944789294No Hit
TGCAGTTGGTCGACAGTCCTCGGTCCGCAACGGTGTGGACATGGCAGAGG52180.6147219908604576No Hit
NGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG52150.6143685669485026No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC52010.6127192553593791No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC49170.5792617916943025No Hit
TGCAGGTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG48000.565478259128056No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC47930.5646536033334943No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG43540.5129359042174075No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG37620.443193585591614No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG36530.43035251679058095No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG35610.4195141834906266No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG30870.3636732054017311No Hit
TGCAGATGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG28210.33233628520838465No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG15660.1844872820405283No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC15260.17977496321446115No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC13660.16092568791019263No Hit
TGCAGCTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG11420.13453670248421667No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA10590.12475864092012737No Hit
TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG9290.10944360473540918No Hit
TTCCAGATCTCGATGCCGACAGGGAAAGCAATAGAATCCAAAGTACCTGT8640.10178608664305008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCACA359.094947E-12148.581761
TGCCGAG359.094947E-12148.581761
TGCACTT100.006591762148.581741
TGCATTT201.8867395E-6148.581741
GTGCAGT100.006591762148.581741
TACAGTT100.006591762148.581741
TGCCGTT151.1159983E-4148.581741
CTCCAGA151.1159983E-4148.581741
GTCCCGC151.1159983E-4148.581741
TCCCGGA3150.0148.581741
TCCCGCG151.1159983E-4148.581741
GCCCGCA100.006591762148.581741
TGAGTTG100.006591762148.581741
TCCCCAG100.006591762148.581741
TGCCGCG253.2077878E-8148.581731
TCCCGTA500.0148.581731
TCCCGCA148400.0148.38151
TGCAGTT208300.0148.332091
TTCCAGA220550.0148.24491
TGCAGGT4650.0146.984091
Produced by FastQC (version 0.11.2)