##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AG1W8 l01n01 mis11.3410000000d205.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	851802
Sequences flagged as poor quality	0
Sequence length	151
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.01703917107497	34.0	34.0	34.0	34.0	34.0
2	33.52227395568454	34.0	34.0	34.0	34.0	34.0
3	33.84705835393671	34.0	34.0	34.0	34.0	34.0
4	33.76467653280927	34.0	34.0	34.0	34.0	34.0
5	33.79159358630292	34.0	34.0	34.0	34.0	34.0
6	36.371147285402	38.0	38.0	38.0	34.0	38.0
7	37.09564898884952	38.0	38.0	38.0	36.0	38.0
8	37.19253887640555	38.0	38.0	38.0	36.0	38.0
9	37.399599907020644	38.0	38.0	38.0	37.0	38.0
10-14	37.244520205399844	38.0	38.0	38.0	36.2	38.0
15-19	37.22034181652543	38.0	38.0	38.0	36.2	38.0
20-24	37.44109922258929	38.0	38.0	38.0	36.6	38.0
25-29	37.13968903571488	38.0	38.0	38.0	36.0	38.0
30-34	37.015283833566954	38.0	38.0	38.0	35.8	38.0
35-39	37.001772947234215	38.0	38.0	38.0	35.8	38.0
40-44	37.0508127475634	38.0	38.0	38.0	36.2	38.0
45-49	36.84412786070003	38.0	37.8	38.0	35.0	38.0
50-54	36.96437012357332	38.0	38.0	38.0	35.8	38.0
55-59	36.57194136665563	38.0	37.6	38.0	34.4	38.0
60-64	36.5390658862036	38.0	37.2	38.0	34.0	38.0
65-69	36.55262232302812	38.0	37.2	38.0	34.2	38.0
70-74	36.551831998516086	38.0	37.2	38.0	34.0	38.0
75-79	36.22039675887119	38.0	37.0	38.0	33.4	38.0
80-84	36.27621395582541	38.0	37.0	38.0	33.8	38.0
85-89	35.96857274343098	38.0	37.0	38.0	32.2	38.0
90-94	36.12989614957466	38.0	37.0	38.0	33.4	38.0
95-99	35.88801904667986	38.0	36.8	38.0	32.0	38.0
100-104	35.741983935233776	38.0	36.8	38.0	31.0	38.0
105-109	35.91232187761945	38.0	36.8	38.0	32.2	38.0
110-114	35.75760211880226	38.0	36.8	38.0	31.0	38.0
115-119	35.25831237775915	38.0	36.0	38.0	28.4	38.0
120-124	35.35778103362048	38.0	36.4	38.0	28.6	38.0
125-129	35.35991298447292	38.0	36.6	38.0	29.0	38.0
130-134	35.19063655638282	38.0	36.2	38.0	28.0	38.0
135-139	35.04670451583819	38.0	36.0	38.0	27.4	38.0
140-144	35.21598563985527	38.0	36.4	38.0	28.0	38.0
145-149	34.77118109607632	37.8	35.4	38.0	25.6	38.0
150-151	32.61913742865126	35.5	31.5	37.5	22.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.8342879778255465
1101	2	0.3990901036233012
1101	3	0.06611120971085427
1101	4	0.0670453067705381
1101	5	0.1255274464133791
1101	6	1.240432179089936
1101	7	0.3706013158078889
1101	8	0.32586816623358317
1101	9	0.172874852557932
1101	10-14	0.2267023401549153
1101	15-19	0.24532028610060763
1101	20-24	0.1366540354909631
1101	25-29	0.25442817932730577
1101	30-34	0.176117609011321
1101	35-39	0.16807105556161872
1101	40-44	0.25472501471041653
1101	45-49	0.23382226082233615
1101	50-54	0.19033737154367003
1101	55-59	0.27558628840576205
1101	60-64	0.33611436850141274
1101	65-69	0.24218854206988993
1101	70-74	0.20278888897630765
1101	75-79	0.3085747566967143
1101	80-84	0.34412177543738665
1101	85-89	0.5105726509877542
1101	90-94	0.3427191528540021
1101	95-99	0.3140102063020578
1101	100-104	0.5265592151540659
1101	105-109	0.3672043262658775
1101	110-114	0.3634207450232054
1101	115-119	0.6462251840090616
1101	120-124	0.4067757413362756
1101	125-129	0.4288900123656276
1101	130-134	0.5263442684291135
1101	135-139	0.5335732444104409
1101	140-144	0.5675406953301732
1101	145-149	0.7006710663954507
1101	150-151	0.7305609187383695
1102	1	0.8682623430231402
1102	2	0.37979856105138765
1102	3	0.06311945201140645
1102	4	0.0735705063199319
1102	5	0.1068498604873156
1102	6	1.1188617517006065
1102	7	0.5203314086007396
1102	8	0.23574885908590915
1102	9	0.23580226256698467
1102	10-14	0.28312465027870815
1102	15-19	0.24549014444291117
1102	20-24	0.14863156557663615
1102	25-29	0.341198033858241
1102	30-34	0.2441102782071667
1102	35-39	0.3130694932531952
1102	40-44	0.3450636203402553
1102	45-49	0.3544663058383932
1102	50-54	0.22796545296780835
1102	55-59	0.4495936472559663
1102	60-64	0.5211868261725598
1102	65-69	0.5200147651919949
1102	70-74	0.3419106379275405
1102	75-79	0.4307223932243147
1102	80-84	0.6236836171419284
1102	85-89	0.7268629433533746
1102	90-94	0.6404608351347605
1102	95-99	0.5358596558625095
1102	100-104	0.9067835906891446
1102	105-109	0.574065287288704
1102	110-114	0.527931030549901
1102	115-119	0.798947645761281
1102	120-124	0.7067563608014495
1102	125-129	0.734655451802162
1102	130-134	0.9168005595700492
1102	135-139	1.0699917862966757
1102	140-144	0.8031218830513254
1102	145-149	1.0080774510411743
1102	150-151	1.4763542819659108
1103	1	0.936101262081074
1103	2	0.4373914522595115
1103	3	0.06552383064224188
1103	4	0.10992324888915306
1103	5	0.1446197295224252
1103	6	1.3253103704660347
1103	7	0.5377138540974684
1103	8	0.3605228013916246
1103	9	0.31749746926142564
1103	10-14	0.2923136952013934
1103	15-19	0.3035639113772035
1103	20-24	0.1554084159721043
1103	25-29	0.34777981100578614
1103	30-34	0.3364610369073944
1103	35-39	0.23810780735354342
1103	40-44	0.3367913044253328
1103	45-49	0.27626891087305694
1103	50-54	0.3123344165835391
1103	55-59	0.3971301811688477
1103	60-64	0.5082440619457387
1103	65-69	0.4192308704047605
1103	70-74	0.32685210694868516
1103	75-79	0.4241119457840483
1103	80-84	0.5757768906806433
1103	85-89	0.5900925365701823
1103	90-94	0.5357082298659819
1103	95-99	0.6315337913325294
1103	100-104	0.7854400344995156
1103	105-109	0.6670850074796206
1103	110-114	0.6785845223286273
1103	115-119	1.0353596018496276
1103	120-124	0.7638180952172959
1103	125-129	0.6209413630546479
1103	130-134	0.7695861702526798
1103	135-139	0.7043130081121731
1103	140-144	0.7203344318511498
1103	145-149	1.0087330858882595
1103	150-151	1.2537435060419
2116	1	0.7184979064046999
2116	2	0.2813464456813719
2116	3	-0.01724291425259139
2116	4	-0.11677555310061649
2116	5	0.08770974818342125
2116	6	1.159411917842391
2116	7	0.09596515183484655
2116	8	0.2227290558527386
2116	9	0.11123750342738248
2116	10-14	0.19539809904955519
2116	15-19	0.24186684337375652
2116	20-24	0.19509442683107636
2116	25-29	0.3396795810920423
2116	30-34	0.33838827553877593
2116	35-39	0.28618344952482033
2116	40-44	0.15272819833288764
2116	45-49	0.3191951458107667
2116	50-54	0.2334236535745191
2116	55-59	0.42163619690867193
2116	60-64	0.4893705475316352
2116	65-69	0.5253994946104754
2116	70-74	0.320341492934034
2116	75-79	0.4406927082609542
2116	80-84	0.6198017206307256
2116	85-89	0.6545384407844068
2116	90-94	0.6177640954187353
2116	95-99	0.6614071644588506
2116	100-104	0.777115982733136
2116	105-109	0.4815897204360766
2116	110-114	0.5352646266246879
2116	115-119	0.9230503174079772
2116	120-124	0.7684481506131107
2116	125-129	0.6891373750696062
2116	130-134	0.7549769410417184
2116	135-139	1.016586548581941
2116	140-144	0.8767680715526467
2116	145-149	0.9908472280604599
2116	150-151	1.824972177551487
1117	1	0.8993130625822303
1117	2	0.3818790614986298
1117	3	0.1085519605624441
1117	4	0.08636419321671696
1117	5	0.11182947815787969
1117	6	1.2519921046812215
1117	7	0.503602870015925
1117	8	0.3137143399022406
1117	9	0.2553143149469008
1117	10-14	0.2578038146393311
1117	15-19	0.29182009685765564
1117	20-24	0.18829763062380067
1117	25-29	0.32315074916600395
1117	30-34	0.2649550936101619
1117	35-39	0.26193636290021516
1117	40-44	0.3507674212364762
1117	45-49	0.43955873800446454
1117	50-54	0.3202325228977685
1117	55-59	0.5264656641456256
1117	60-64	0.5680102607215147
1117	65-69	0.4407865336787822
1117	70-74	0.4153127456670518
1117	75-79	0.44151390099646903
1117	80-84	0.5435701523771286
1117	85-89	0.5753847367671554
1117	90-94	0.4643975364776409
1117	95-99	0.015078422345034426
1117	100-104	0.5785901543627858
1117	105-109	0.6315300174052823
1117	110-114	0.7175426174815129
1117	115-119	1.070972121897114
1117	120-124	0.9239437004920177
1117	125-129	0.7709450297195986
1117	130-134	0.8098531547947516
1117	135-139	0.6429818400667173
1117	140-144	0.760511326435271
1117	145-149	-0.32101335689673505
1117	150-151	0.4946544852834407
2117	1	-30.95102999059101
2117	2	-10.51733504986171
2117	3	-1.8283996491485937
2117	4	-0.3659275009381844
2117	5	0.014680283013397855
2117	6	1.1819482334581082
2117	7	-0.0763400644081429
2117	8	0.3014741037233222
2117	9	0.3062420428793473
2117	10-14	0.44084709211657724
2117	15-19	0.43534117483628165
2117	20-24	0.30656851068712854
2117	25-29	0.40494617072558015
2117	30-34	0.5762596852449491
2117	35-39	0.3428304452329769
2117	40-44	0.23309671929690978
2117	45-49	0.4873744128956119
2117	50-54	0.36343066909118704
2117	55-59	0.5306796105765557
2117	60-64	0.7035645884692343
2117	65-69	0.5350524328739255
2117	70-74	0.5465337180908563
2117	75-79	0.6169534378746917
2117	80-84	0.6979277523243539
2117	85-89	0.7701657105103905
2117	90-94	0.6852409854814638
2117	95-99	0.1769977058916794
2117	100-104	0.9916731798278917
2117	105-109	0.8696682942228051
2117	110-114	0.9351519656804896
2117	115-119	1.2843935825119814
2117	120-124	1.007990078642166
2117	125-129	0.7398434661888018
2117	130-134	0.9857383309393697
2117	135-139	0.8975348813650399
2117	140-144	0.9964994185318545
2117	145-149	1.2083282448977073
2117	150-151	1.848581454540593
1116	1	0.3682682550230254
1116	2	0.22474490965245764
1116	3	-0.09612063430379436
1116	4	-0.20495415410957918
1116	5	0.09113945980152494
1116	6	1.2500150350775385
1116	7	-0.11380632621650477
1116	8	0.23613166107825379
1116	9	0.10699271764317331
1116	10-14	0.2366905469074041
1116	15-19	0.2606060196405977
1116	20-24	0.10301250528901562
1116	25-29	0.04337734481465816
1116	30-34	0.1638069187132345
1116	35-39	0.2405716733031511
1116	40-44	0.16023403373685596
1116	45-49	0.3389331173490575
1116	50-54	0.2712494950021238
1116	55-59	0.4016546443147462
1116	60-64	0.5312585830711214
1116	65-69	0.42738880264450785
1116	70-74	0.35515551296264647
1116	75-79	0.4113613326115342
1116	80-84	0.4751089264134265
1116	85-89	0.5177230654361722
1116	90-94	0.5656897034301807
1116	95-99	-0.31667807008673066
1116	100-104	0.7920527344837609
1116	105-109	0.5445198461796252
1116	110-114	0.6395598604173216
1116	115-119	0.9944357552515157
1116	120-124	0.8620487830956023
1116	125-129	0.6187925215194241
1116	130-134	0.6128351595493555
1116	135-139	0.8764788759669315
1116	140-144	0.604034843633066
1116	145-149	0.7661420100798892
1116	150-151	0.8596307123003868
2118	1	0.8377740806558123
2118	2	0.3709091433920264
2118	3	0.031082392346803545
2118	4	-0.030640507397379224
2118	5	0.08069572961090898
2118	6	1.2822100263725673
2118	7	0.5393732193394953
2118	8	0.3298060506144296
2118	9	0.17095749600128585
2118	10-14	0.2865014928473073
2118	15-19	0.22349272112713692
2118	20-24	0.16986041386452655
2118	25-29	0.2887748717107712
2118	30-34	0.34693512727535136
2118	35-39	0.17735440621686394
2118	40-44	0.19165294360694674
2118	45-49	0.3198866462093761
2118	50-54	0.12856189177012567
2118	55-59	0.2895210342080503
2118	60-64	0.41058740767716984
2118	65-69	0.37516881246549616
2118	70-74	0.3298501694511913
2118	75-79	0.4413756176880881
2118	80-84	0.4644971233689148
2118	85-89	0.3177232886404866
2118	90-94	0.4703886008008311
2118	95-99	0.5329335543943188
2118	100-104	0.7325565066367972
2118	105-109	0.4919590230020816
2118	110-114	0.6759821183522376
2118	115-119	0.9996198988165332
2118	120-124	0.7940710142460006
2118	125-129	0.5671934857620968
2118	130-134	0.5067999379074593
2118	135-139	0.6637254274601645
2118	140-144	0.5638969033625472
2118	145-149	0.5150947354046451
2118	150-151	1.3204293373617588
1119	1	0.790764320131089
1119	2	0.31594322820595266
1119	3	-0.014160674228548942
1119	4	-0.014474673131424254
1119	5	0.1132842638247098
1119	6	1.2801198375651595
1119	7	0.14968459474015106
1119	8	0.3581785726679456
1119	9	0.10195509623736143
1119	10-14	0.1837447524431184
1119	15-19	0.20644136840638083
1119	20-24	0.004603353306215752
1119	25-29	0.1921381417742296
1119	30-34	0.1746854553173236
1119	35-39	0.24313952619577606
1119	40-44	0.057140440892595734
1119	45-49	0.16699484716593815
1119	50-54	0.22508169585435667
1119	55-59	0.3756833978177525
1119	60-64	0.4023133260555056
1119	65-69	0.2775404399434436
1119	70-74	0.2728396850997683
1119	75-79	0.3091881205880327
1119	80-84	0.30114543453274223
1119	85-89	0.40731518029662794
1119	90-94	0.3849603088277007
1119	95-99	0.35714856433300923
1119	100-104	0.6525865361652095
1119	105-109	0.4416039952832378
1119	110-114	0.5066754886564127
1119	115-119	0.8489771963816892
1119	120-124	0.6613796595208044
1119	125-129	0.3769064606341743
1119	130-134	0.6213861595877432
1119	135-139	0.47524696687253254
1119	140-144	0.47304440254127655
1119	145-149	0.729588975414444
1119	150-151	1.2273876319518067
2119	1	0.8619321668687547
2119	2	0.30620423979600275
2119	3	0.04692344283119354
2119	4	-0.01811929837519699
2119	5	0.04950541601436953
2119	6	1.2264395674829345
2119	7	0.42829531217699923
2119	8	0.24045238023150262
2119	9	0.09196806924532552
2119	10-14	0.18368996694509576
2119	15-19	0.1346418323127736
2119	20-24	0.16272252413779853
2119	25-29	0.1819810855715076
2119	30-34	0.1490954499587005
2119	35-39	0.19059197198373568
2119	40-44	-0.16524733497418254
2119	45-49	0.17253903296454354
2119	50-54	0.11572317921265096
2119	55-59	0.28367798779741804
2119	60-64	0.3144201025723561
2119	65-69	0.30226773551386543
2119	70-74	0.19114144887681306
2119	75-79	0.1804565914197056
2119	80-84	0.21443689509546005
2119	85-89	0.3775440020752825
2119	90-94	0.3656926032416905
2119	95-99	0.35870977970303386
2119	100-104	0.7324507336138169
2119	105-109	0.42315475991022566
2119	110-114	0.49715229190928056
2119	115-119	0.9073123682716684
2119	120-124	0.5455068083239638
2119	125-129	0.5450662637261843
2119	130-134	0.6096721901423621
2119	135-139	0.43534069994836955
2119	140-144	0.4428038736461204
2119	145-149	0.5061202614935496
2119	150-151	0.621585185259633
1118	1	0.8825884867400404
1118	2	0.4142669933047074
1118	3	0.0997008805612154
1118	4	0.1342456417873521
1118	5	0.1604166948051997
1118	6	1.2943939464277676
1118	7	0.5178833020457674
1118	8	0.4916386921289302
1118	9	0.18435491055059572
1118	10-14	0.26217334071827025
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2104	150-151	-0.43952160995883105
2111	1	0.8848320783745081
2111	2	0.34743395986419756
2111	3	0.06210985571435401
2111	4	0.10991577934474606
2111	5	0.07292409380031728
2111	6	0.8925074730690667
2111	7	0.3134985739649778
2111	8	-0.15026882289028975
2111	9	-0.06416578205434575
2111	10-14	-0.2211467186703402
2111	15-19	-0.19930626106115312
2111	20-24	-0.15337224970190277
2111	25-29	-0.034271777992366026
2111	30-34	-0.10479380723958087
2111	35-39	-0.26113794557161896
2111	40-44	-0.23767582712218882
2111	45-49	-0.20504026438113243
2111	50-54	-0.42559563506442544
2111	55-59	-0.40531994733679966
2111	60-64	-0.4149307696209519
2111	65-69	-0.574600308063296
2111	70-74	-0.42851115370401516
2111	75-79	-0.5903023092517401
2111	80-84	-0.29278157570394114
2111	85-89	-0.6857149260943771
2111	90-94	-0.8520114459951031
2111	95-99	-0.5226417724460788
2111	100-104	-0.5104366787043872
2111	105-109	-0.5456791858833014
2111	110-114	-0.836368149262043
2111	115-119	-2.1812750735446187
2111	120-124	-1.2986372423127506
2111	125-129	-1.056470636375309
2111	130-134	-0.6946221907228747
2111	135-139	-0.6393311663803161
2111	140-144	-1.273309378992458
2111	145-149	-1.566089084898941
2111	150-151	-3.037985742630049
2110	1	0.9547096267678299
2110	2	0.32972133227691813
2110	3	0.0695204574596815
2110	4	0.010384238535593227
2110	5	0.06525796885998147
2110	6	1.1450072987511888
2110	7	0.10313111187241475
2110	8	-0.17163086235586178
2110	9	-0.13901734367437513
2110	10-14	-0.155823104229313
2110	15-19	-0.19658733121202943
2110	20-24	-0.3261902028345318
2110	25-29	-0.21711949925538931
2110	30-34	-0.011953369782155221
2110	35-39	-0.3653875902313217
2110	40-44	-0.24604372903218064
2110	45-49	-0.20699168064685836
2110	50-54	-0.21882995584151388
2110	55-59	-0.5034345895024188
2110	60-64	-0.2101443117066566
2110	65-69	-0.3285532690536357
2110	70-74	-0.25078397996004753
2110	75-79	-0.47795148513043983
2110	80-84	-0.5110038765926888
2110	85-89	-0.46215221188452915
2110	90-94	-0.5217360237435642
2110	95-99	-0.8222551836592729
2110	100-104	-0.2361510870158554
2110	105-109	-0.4827043681125289
2110	110-114	-0.9250516040815384
2110	115-119	-6.476933861043069
2110	120-124	-3.164431311213896
2110	125-129	-0.6141312367797269
2110	130-134	-0.8213270249599844
2110	135-139	-1.0311419376162618
2110	140-144	-0.8740703704052919
2110	145-149	-0.9270222701961686
2110	150-151	-1.8162409547346812
2109	1	0.9607347152913448
2109	2	0.3954670581609889
2109	3	0.057340223723628014
2109	4	0.05322560902374818
2109	5	0.014302760209403687
2109	6	1.0108042630494296
2109	7	0.4603680803882426
2109	8	-0.057710788182156136
2109	9	-0.0982814973059618
2109	10-14	-0.03851044577037044
2109	15-19	-0.051668539305786965
2109	20-24	-0.35695407783545363
2109	25-29	-0.07747625978507955
2109	30-34	-0.1570641314612189
2109	35-39	-0.505153915177857
2109	40-44	-0.1595883162557712
2109	45-49	-0.45954543196540243
2109	50-54	-0.3545308873262485
2109	55-59	-0.6532740991124584
2109	60-64	-0.4808695313325302
2109	65-69	-0.5581495530486507
2109	70-74	-0.5088174095396809
2109	75-79	-0.5883511839740478
2109	80-84	-0.6099977127334597
2109	85-89	-0.7431622360528465
2109	90-94	-0.8413504459629095
2109	95-99	-0.5129948424096966
2109	100-104	-0.5922351912128363
2109	105-109	-0.5545039387729602
2109	110-114	-1.606469182778902
2109	115-119	-5.049705480190028
2109	120-124	-1.8454960594028051
2109	125-129	-1.3652474804462642
2109	130-134	-1.3107116977900688
2109	135-139	-1.5683149713965818
2109	140-144	-0.984012234847448
2109	145-149	-0.976223216712583
2109	150-151	-2.531339359939043
2108	1	0.7868287290557916
2108	2	0.11551114643647509
2108	3	0.07514403398946001
2108	4	0.005758056881475682
2108	5	-0.08945772853355294
2108	6	0.5841790875929362
2108	7	-0.2171088706570785
2108	8	-0.47595733624950753
2108	9	-0.4956417659167158
2108	10-14	-0.36139182790380175
2108	15-19	-0.45215394318036317
2108	20-24	-0.5976299950014408
2108	25-29	-0.35059848880913336
2108	30-34	-4.822229447035575
2108	35-39	-1.0571116077471459
2108	40-44	-0.7019705671693828
2108	45-49	-0.38674598105921376
2108	50-54	-0.41763339102088537
2108	55-59	-0.43097983924515404
2108	60-64	-0.7160616214136013
2108	65-69	-0.6856308325361624
2108	70-74	-0.6737517718055699
2108	75-79	-0.906653074266579
2108	80-84	-0.7751472604112521
2108	85-89	-1.2361418119891852
2108	90-94	-1.205546499660322
2108	95-99	-1.1286566921385486
2108	100-104	-0.9140440472055644
2108	105-109	-1.0542491790324178
2108	110-114	-1.469436886416652
2108	115-119	-1.287984367904052
2108	120-124	-1.4176700504120134
2108	125-129	-1.2300909454260847
2108	130-134	-1.5003070502798508
2108	135-139	-1.9666581055597376
2108	140-144	-1.8688837110317351
2108	145-149	-2.1302063110540743
2108	150-151	-2.7669581107506254
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	4.0
17	17.0
18	43.0
19	100.0
20	291.0
21	609.0
22	1351.0
23	2197.0
24	2778.0
25	3401.0
26	4309.0
27	5592.0
28	7320.0
29	9727.0
30	12927.0
31	17265.0
32	24282.0
33	31558.0
34	42459.0
35	67848.0
36	134845.0
37	482878.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.11552346570397112	0.0779783393501805	99.64079422382672	0.16570397111913357
2	55.1796074674631	0.043672120985862914	28.098666121939136	16.678054289611904
3	0.06973451576774883	0.09814487404349838	0.10237120833245285	99.72974940185631
4	0.34174608653184657	22.84638918434096	0.1568439613900883	76.6550207677371
5	71.44712034017296	28.216768685680478	0.15895712853456553	0.17715384561200842
6	29.0701360175252	32.64408864970967	22.072265620414132	16.213509712350994
7	0.6456899608124893	44.22154444342699	22.84263244275078	32.290133153009734
8	71.47001298423812	0.28269480466117713	27.89157574178037	0.355716469320335
9	54.908652480271236	16.61477667345228	0.597204514664206	27.879366331612275
10-14	16.889488402234324	45.944198299604835	22.3674750704976	14.798838227663236
15-19	32.071044679397325	20.17541635262655	20.824111706711186	26.929427261264944
20-24	33.558338674950285	25.292661909692626	9.164923303772472	31.98407611158462
25-29	22.533076935719805	44.90029373023308	6.809516765633328	25.75711256841379
30-34	22.119764921894994	17.20878795776483	36.355444105555044	24.316003014785124
35-39	40.73937370421765	21.37588312776913	21.014625464603277	16.87011770340995
40-44	12.434509428247411	31.411079100542143	29.78725102782102	26.367160443389427
45-49	15.05866386789418	28.53611520048086	21.070906149551185	35.33431478207377
50-54	29.632449794670592	26.605549176921393	16.06363920253768	27.698361825870332
55-59	20.023690951653084	42.027865630745175	32.10971563814125	5.838727779460485
60-64	34.62241225073433	20.29610167621114	23.130868441257476	21.950617631797062
65-69	18.265535887448024	36.113298630432894	25.6326000643342	19.988565417784883
70-74	24.142136318064527	14.060943740446724	31.470764332556158	30.32615560893259
75-79	29.95644527718883	19.15569580724159	25.616539994036174	25.27131892153341
80-84	22.724975992073276	22.046531940521387	34.618749427683895	20.609742639721436
85-89	29.440855973571324	21.151441297390708	35.598484154768364	13.809218574269607
90-94	24.33034907173263	37.30406831634582	28.74980335805739	9.615779253864162
95-99	15.749669524138238	32.25435018936326	31.620423525655024	20.375556760843484
100-104	9.515169018152106	40.02049772130143	28.992958457481905	21.47137480306456
105-109	20.601172572968835	26.920411081448503	32.20485511891261	20.273561226670047
110-114	41.424626849901735	25.746734569770908	9.989551562452307	22.839087017875045
115-119	9.931415986344245	42.764374819500304	15.930861866959692	31.373347327195756
120-124	5.207242997785871	20.455410999269784	46.44412668671827	27.89321931622607
125-129	20.413734647253705	29.638765816469085	17.087586082211594	32.85991345406561
130-134	16.511466279722285	31.41410797344923	45.97204514664206	6.102380600186429
135-139	33.47864879396855	0.9602231504504568	45.900033106285264	19.66109494929573
140-144	13.345073150802653	40.93749486382986	16.056900547310292	29.660531438057202
145-149	27.290097933557327	18.463586608155417	32.89870181098424	21.347613647303014
150-151	40.411621480109226	15.602041319461565	27.089981004975332	16.896356195453873
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	0.5
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	0.5
19	0.5
20	0.5
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.5
28	2.5
29	5.5
30	8.5
31	16.0
32	44.0
33	150.0
34	584.5
35	5295.0
36	122134.5
37	130532.5
38	14369.0
39	1432.5
40	458.5
41	319.0
42	301.0
43	388.5
44	606.0
45	1293.0
46	6403.5
47	56505.5
48	60342.0
49	23455.5
50	110033.5
51	107876.5
52	14287.5
53	4134.0
54	2484.0
55	921.5
56	410.0
57	245.0
58	285.5
59	544.0
60	1390.5
61	7416.0
62	81056.0
63	84246.5
64	10482.0
65	1065.5
66	188.0
67	45.5
68	22.0
69	8.5
70	4.0
71	1.0
72	1.0
73	1.5
74	1.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.442116829967528
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	851802.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	1.371576005890804
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.52595232682498	1.0084643202174426
2	9.57750341792356	0.2627254776872224
3	3.535593406175245	0.14548005247487114
4	2.0642789971466198	0.11325262167802541
5	1.2766853399542069	0.08755354896768672
6	0.889036417598065	0.07316286112443737
7	0.8351900590254177	0.0801868651722451
8	0.49029989537636015	0.05379868577511897
9	0.44711557781928746	0.05519276985572434
>10	3.9714699792061334	1.2118096241863936
>50	1.358188284533296	1.3546989705490458
>100	1.7020334198581812	4.341521339645562
>500	0.10315354059746552	0.9888477167967664
>1k	0.15473031089619826	5.495078757213174
>5k	0.0343845135324885	2.62462609080873
>10k+	0.0343845135324885	82.10360029784755
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	237368	27.866569930570716	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	192854	22.64070758227851	No Hit
TGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	155935	18.306484370781003	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	110212	12.93868762928474	No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	6391	0.7502917344641125	No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC	5675	0.6662346413837957	No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	5178	0.6078877485612854	No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG	5017	0.5889866424356834	No Hit
TGCAGTTGGTCGACAGTCCTCGGTCCGCAACGGTGTGGACATGGCAGAGG	4273	0.5016424004639576	No Hit
NGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	4236	0.4972986680003099	No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	4124	0.48415007243467384	No Hit
TGCAGGTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	4041	0.47440602393513986	No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG	3267	0.38353983672261865	No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	3099	0.3638169433741644	No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	3029	0.3555990711456418	No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC	2931	0.3440940500257102	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG	2621	0.3077006158708244	No Hit
TGCAGTTGGTCGACAGTCCTCGGCACGTGAGAAAACGAGAAAGTGAGCTG	2367	0.27788147949875674	No Hit
TGCAGATGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	2304	0.2704853944930864	No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC	1922	0.2256392917602917	No Hit
TGCAGTTGGTCGACAGTCCTCGGTACGTGAGAAAACGAGAAAGTGAGCTG	1807	0.21213850167057605	No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	1562	0.18337594887074696	No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	1403	0.16470963909453137	No Hit
TTCCAGATCTCGATGCCGACAGGGAAAGCAATAGAATCCAAAGTACCTGT	1400	0.16435744457045182	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA	1119	0.13136855748166829	No Hit
TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	1102	0.12937278851188422	No Hit
TGCAGCTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	959	0.11258484953075949	No Hit
TTCCATATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	945	0.11094127508505498	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	1.1739817469317987E-4
4	0.0	0.0	1.1739817469317987E-4
5	0.0	0.0	8.217872228522591E-4
6	0.0	0.0	8.217872228522591E-4
7	0.0	0.0	8.217872228522591E-4
8	0.0	0.0	8.217872228522591E-4
9	0.0	0.0	8.217872228522591E-4
10-11	0.0	0.0	8.217872228522591E-4
12-13	0.0	0.0	8.217872228522591E-4
14-15	0.0	0.0	8.217872228522591E-4
16-17	0.0	0.0	8.217872228522591E-4
18-19	0.0	0.0	8.80486310198849E-4
20-21	0.0	0.0	9.97884484892029E-4
22-23	0.0	0.0	0.0011739817469317987
24-25	0.0	0.0	0.0011739817469317987
26-27	0.0	0.0	0.0011739817469317987
28-29	0.0	0.0	0.0011739817469317987
30-31	0.0	0.0	0.0011739817469317987
32-33	0.0	0.0	0.001702273533051108
34-35	0.0	0.0	0.0021718662318238272
36-37	0.0	0.0	0.005517714210579454
38-39	0.0	0.0	0.00892226127668167
40-41	0.0	0.0	0.01038973846034642
42-43	0.0	0.0	0.03457376244714147
44-45	0.0	0.0	0.042380741064237934
46-47	0.0	0.0	0.19558535903883767
48-49	0.0	0.0	1.0231250924510626
50-51	0.0	0.0	1.092624811869425
52-53	0.0	0.0	1.0962641552849137
54-55	0.0	0.0	1.0962641552849137
56-57	0.0	0.0	1.096909845245726
58-59	0.0	0.0	1.097203340682459
60-61	0.0	0.0	1.123265735464345
62-63	0.0	0.0	1.1289008478496176
64-65	0.0	0.0	1.1289595469369642
66-67	0.0	0.0	1.1290182460243108
68-69	0.0	0.0	1.1293704405483904
70-71	0.0	0.0	1.130427024120629
72-73	0.0	0.0	1.1312488113434813
74-75	0.0	0.0	1.1314836076928676
76-77	0.0	0.0	1.1375883127769129
78-79	0.0	0.0	1.1475084585384865
80-81	0.0	0.0	1.1562546225531285
82-83	0.0	0.0	1.156313321640475
84-85	0.0	0.0	1.1563720207278216
86-87	0.0	0.0	1.156489418902515
88-89	0.0	0.0	1.156489418902515
90-91	0.0	0.0	1.156489418902515
92-93	0.0	0.0	1.156489418902515
94-95	0.0	0.0	1.1569590116012876
96-97	0.0	0.0	1.157193807950674
98-99	0.0	0.0	1.157193807950674
100-101	0.0	0.0	1.157193807950674
102-103	0.0	0.0	1.1655290783538899
104-105	0.0	0.0	1.173512154233026
106-107	0.0	0.0	1.1735708533203726
108-109	0.0	0.0	1.1738643487571054
110-111	0.0	0.0	1.1741578441938385
112-113	0.0	0.0	1.1742752423685316
114-115	0.0	0.0	1.1744513396305714
116-117	0.0	0.0	1.1744513396305714
118-119	0.0	0.0	1.1750383305040373
120-121	0.0	0.0	1.176681904949742
122-123	0.0	0.0	1.2818119703874844
124-125	0.0	0.0	1.3776675800244658
126-127	0.0	0.0	1.3776675800244658
128-129	0.0	0.0	1.3779610754611986
130-131	0.0	0.0	1.3781958718105851
132-133	0.0	0.0	1.378489367247318
134-135	0.0	0.0	1.378489367247318
136-137	0.0	0.0	1.3785480663346648
138-139	0.0	0.0	1.3786067654220113
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAGTTG	20	1.885317E-6	148.60434	1
TTCCGCA	20	1.885317E-6	148.60434	1
TGCGGTT	20	1.885317E-6	148.60434	1
CCCCGCA	10	0.00658877	148.60434	1
TCCCGAA	165	0.0	148.60434	1
TGCAGCT	95	0.0	148.60434	1
TGCAGAC	20	1.885317E-6	148.60434	1
AGCAGTT	10	0.00658877	148.60434	1
TGCGTTG	20	1.885317E-6	148.60434	1
TTCTAGA	30	5.475158E-10	148.60432	1
TCCCGTA	60	0.0	148.60432	1
TCCCGGA	300	0.0	148.60432	1
TCCCGCA	12700	0.0	148.60432	1
TGCAGGT	435	0.0	148.60432	1
TCCCACA	30	5.475158E-10	148.60432	1
TGCAGTT	17920	0.0	148.397	1
TTCCAGA	22885	0.0	148.18225	1
GCCGGCG	420	0.0	144.9756	2
GTTGACA	15	1.2307675E-4	144.97559	9
CAGGTGG	435	0.0	144.97559	3
>>END_MODULE