FastQCFastQC Report
Wed 17 Jun 2015
000000000-AG1W8 l01n01 mis11.3410000000d205.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG1W8 l01n01 mis11.3410000000d205.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences851802
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG23736827.866569930570716No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC19285422.64070758227851No Hit
TGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG15593518.306484370781003No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG11021212.93868762928474No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG63910.7502917344641125No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC56750.6662346413837957No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC51780.6078877485612854No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG50170.5889866424356834No Hit
TGCAGTTGGTCGACAGTCCTCGGTCCGCAACGGTGTGGACATGGCAGAGG42730.5016424004639576No Hit
NGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG42360.4972986680003099No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG41240.48415007243467384No Hit
TGCAGGTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG40410.47440602393513986No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG32670.38353983672261865No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG30990.3638169433741644No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG30290.3555990711456418No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC29310.3440940500257102No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG26210.3077006158708244No Hit
TGCAGTTGGTCGACAGTCCTCGGCACGTGAGAAAACGAGAAAGTGAGCTG23670.27788147949875674No Hit
TGCAGATGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG23040.2704853944930864No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC19220.2256392917602917No Hit
TGCAGTTGGTCGACAGTCCTCGGTACGTGAGAAAACGAGAAAGTGAGCTG18070.21213850167057605No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC15620.18337594887074696No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG14030.16470963909453137No Hit
TTCCAGATCTCGATGCCGACAGGGAAAGCAATAGAATCCAAAGTACCTGT14000.16435744457045182No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA11190.13136855748166829No Hit
TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG11020.12937278851188422No Hit
TGCAGCTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG9590.11258484953075949No Hit
TTCCATATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC9450.11094127508505498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGTTG201.885317E-6148.604341
TTCCGCA201.885317E-6148.604341
TGCGGTT201.885317E-6148.604341
CCCCGCA100.00658877148.604341
TCCCGAA1650.0148.604341
TGCAGCT950.0148.604341
TGCAGAC201.885317E-6148.604341
AGCAGTT100.00658877148.604341
TGCGTTG201.885317E-6148.604341
TTCTAGA305.475158E-10148.604321
TCCCGTA600.0148.604321
TCCCGGA3000.0148.604321
TCCCGCA127000.0148.604321
TGCAGGT4350.0148.604321
TCCCACA305.475158E-10148.604321
TGCAGTT179200.0148.3971
TTCCAGA228850.0148.182251
GCCGGCG4200.0144.97562
GTTGACA151.2307675E-4144.975599
CAGGTGG4350.0144.975593
Produced by FastQC (version 0.11.2)