##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AG1W8 l01n01 mis10.3410000000d1f7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 871084 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.029227950461724 34.0 34.0 34.0 34.0 34.0 2 33.528727424680056 34.0 34.0 34.0 34.0 34.0 3 33.852934963792244 34.0 34.0 34.0 34.0 34.0 4 33.7535335283394 34.0 34.0 34.0 34.0 34.0 5 33.78899509117375 34.0 34.0 34.0 34.0 34.0 6 36.36601751381038 38.0 38.0 38.0 34.0 38.0 7 37.09197276037673 38.0 38.0 38.0 36.0 38.0 8 37.20968701066717 38.0 38.0 38.0 36.0 38.0 9 37.419967534703886 38.0 38.0 38.0 37.0 38.0 10-14 37.27391824439434 38.0 38.0 38.0 36.4 38.0 15-19 37.222282351644616 38.0 38.0 38.0 36.2 38.0 20-24 37.438065904091914 38.0 38.0 38.0 36.6 38.0 25-29 37.146652446836356 38.0 38.0 38.0 36.0 38.0 30-34 36.93709837398001 38.0 38.0 38.0 35.4 38.0 35-39 37.00230540338246 38.0 38.0 38.0 35.8 38.0 40-44 37.07115984221957 38.0 38.0 38.0 36.2 38.0 45-49 36.82836305109496 38.0 37.8 38.0 35.0 38.0 50-54 36.99000073471675 38.0 38.0 38.0 36.0 38.0 55-59 36.58786041300265 38.0 37.6 38.0 34.2 38.0 60-64 36.540260870363824 38.0 37.2 38.0 34.0 38.0 65-69 36.6051526603634 38.0 37.2 38.0 34.4 38.0 70-74 36.59494193441734 38.0 37.4 38.0 34.2 38.0 75-79 36.28396733265678 38.0 37.0 38.0 33.4 38.0 80-84 36.32043637582598 38.0 37.0 38.0 33.8 38.0 85-89 36.01961601866181 38.0 37.0 38.0 32.4 38.0 90-94 36.15304861528854 38.0 37.0 38.0 33.4 38.0 95-99 35.923994471256506 38.0 36.8 38.0 32.0 38.0 100-104 35.73740374062662 38.0 36.8 38.0 31.0 38.0 105-109 35.91872884819374 38.0 37.0 38.0 32.0 38.0 110-114 35.733075111011104 38.0 36.8 38.0 30.8 38.0 115-119 35.239863664124236 38.0 35.8 38.0 28.2 38.0 120-124 35.198058970202645 38.0 36.4 38.0 27.8 38.0 125-129 35.03576463349115 38.0 36.4 38.0 27.2 38.0 130-134 34.79187472161124 38.0 35.8 38.0 25.6 38.0 135-139 34.73388261063227 38.0 36.0 38.0 25.4 38.0 140-144 34.89834068815407 38.0 36.2 38.0 26.4 38.0 145-149 34.53271050782703 37.8 35.4 38.0 24.0 38.0 150-151 32.50740571517787 36.0 31.5 37.5 20.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8581898262950958 1101 2 0.3688991269186346 1101 3 0.08003254938181215 1101 4 0.05363506711874777 1101 5 0.11725569614571896 1101 6 1.263621542955967 1101 7 0.42948918262347746 1101 8 0.2890919475831808 1101 9 0.14270918178488756 1101 10-14 0.2411777548523517 1101 15-19 0.3051863289326917 1101 20-24 0.14932136929168394 1101 25-29 0.29841785242433616 1101 30-34 0.2246335777825479 1101 35-39 0.19180482946656952 1101 40-44 0.2583949679431967 1101 45-49 0.29253613812529267 1101 50-54 0.21367955404592465 1101 55-59 0.3414937941509706 1101 60-64 0.43249397361566366 1101 65-69 0.3196052672406893 1101 70-74 0.2490933265694153 1101 75-79 0.3679876111774121 1101 80-84 0.3870918006913513 1101 85-89 0.5018998088050424 1101 90-94 0.4554965898034311 1101 95-99 0.43584632824646974 1101 100-104 0.6441804350944977 1101 105-109 0.46232468409017713 1101 110-114 0.4857284846045289 1101 115-119 0.6828943373713017 1101 120-124 0.4331842342507102 1101 125-129 0.4472173036868199 1101 130-134 0.5858660287499617 1101 135-139 0.6340079026275305 1101 140-144 0.5038132421834547 1101 145-149 0.6241395780934624 1101 150-151 0.5221970370283273 1102 1 0.8975017956911202 1102 2 0.3708656825386356 1102 3 0.06833255233086533 1102 4 0.06011850936887697 1102 5 0.11800520511042123 1102 6 1.043945397743002 1102 7 0.41744012755491866 1102 8 0.20059918665670295 1102 9 0.10994049808370931 1102 10-14 0.1993082801312056 1102 15-19 0.20267919902602927 1102 20-24 0.1417440854493961 1102 25-29 0.2203458529996638 1102 30-34 0.09711792406535125 1102 35-39 0.2068372613368723 1102 40-44 0.2036508055677544 1102 45-49 0.19849601059495114 1102 50-54 0.09993165223125544 1102 55-59 0.28784664662239123 1102 60-64 0.3589292720731976 1102 65-69 0.23387596276810996 1102 70-74 0.12856459335166193 1102 75-79 0.19587179358920537 1102 80-84 0.36674185623192557 1102 85-89 0.4636953053012718 1102 90-94 0.3594521035318081 1102 95-99 0.3421769886301007 1102 100-104 0.6526421947021745 1102 105-109 0.2675397557393353 1102 110-114 0.237122328110992 1102 115-119 0.46578563115036076 1102 120-124 0.4453443532339634 1102 125-129 0.4412933200380422 1102 130-134 0.4712712128478955 1102 135-139 0.5465792167843944 1102 140-144 0.3690315940122062 1102 145-149 0.4586732812489345 1102 150-151 0.9711364370038069 1103 1 0.8806912714864126 1103 2 0.36988322089646175 1103 3 0.051880432373245355 1103 4 0.07895048252775894 1103 5 0.14066394861652753 1103 6 1.2420088947954397 1103 7 0.49202653640386274 1103 8 0.26654084306533576 1103 9 0.19776441379710974 1103 10-14 0.14069462555939793 1103 15-19 0.2674624117071005 1103 20-24 0.09941650886761977 1103 25-29 0.2641348718995431 1103 30-34 0.265459978918706 1103 35-39 0.1877204198023037 1103 40-44 0.21696237330982626 1103 45-49 0.2373949115469287 1103 50-54 0.21972589179299007 1103 55-59 0.41451318262084413 1103 60-64 0.47671084778486517 1103 65-69 0.3590186507089612 1103 70-74 0.33811120147897356 1103 75-79 0.34829572981284684 1103 80-84 0.4892861833822053 1103 85-89 0.5594528000275787 1103 90-94 0.4019018665615093 1103 95-99 0.5098620156976708 1103 100-104 0.6484055694981734 1103 105-109 0.42861442947423 1103 110-114 0.45450993915418536 1103 115-119 0.9033823416713815 1103 120-124 0.7391718550898574 1103 125-129 0.3494359215278706 1103 130-134 0.4926393510603333 1103 135-139 0.455526512809854 1103 140-144 0.4780592509899364 1103 145-149 0.6640072744567647 1103 150-151 0.6911944571913438 2116 1 0.753834486428012 2116 2 0.3467179225791739 2116 3 -0.07045079800537479 2116 4 -0.1695397633482969 2116 5 0.0675187803542272 2116 6 1.2174990607300344 2116 7 0.07050119550418543 2116 8 0.16539807811094676 2116 9 0.08475775767596616 2116 10-14 0.2392056525973345 2116 15-19 0.29402854987993976 2116 20-24 0.18146353831898665 2116 25-29 0.3007484215064693 2116 30-34 0.2889499625433629 2116 35-39 0.1797492435813055 2116 40-44 0.1030630858016437 2116 45-49 0.289795271392002 2116 50-54 0.25736869671811036 2116 55-59 0.4000823025015592 2116 60-64 0.4689862602158783 2116 65-69 0.4057291682195725 2116 70-74 0.3264198898132662 2116 75-79 0.33427747060501645 2116 80-84 0.4831447622401157 2116 85-89 0.5653048764979189 2116 90-94 0.46993920971255676 2116 95-99 0.5503658566857936 2116 100-104 0.6625995913963507 2116 105-109 0.5033324549708524 2116 110-114 0.5950454673447609 2116 115-119 0.7856739702814508 2116 120-124 0.7117485346834087 2116 125-129 0.5088913501859125 2116 130-134 0.6281206981136904 2116 135-139 0.6913570340245485 2116 140-144 0.6430244547520374 2116 145-149 0.822037791837154 2116 150-151 1.5300911674013875 1117 1 0.9072618485078721 1117 2 0.3390074442934363 1117 3 0.11825133510711794 1117 4 0.09716536181818469 1117 5 0.12329243830794212 1117 6 1.2752822463354079 1117 7 0.437216642571272 1117 8 0.32573611016368886 1117 9 0.2617362871961362 1117 10-14 0.255130288268532 1117 15-19 0.25348393897760957 1117 20-24 0.1615906737432553 1117 25-29 0.3584575933398 1117 30-34 0.34401717257069464 1117 35-39 0.27373013008987357 1117 40-44 0.31494086930968734 1117 45-49 0.4948188310838191 1117 50-54 0.3463531903325219 1117 55-59 0.5355725733226606 1117 60-64 0.626438348158473 1117 65-69 0.543463683376558 1117 70-74 0.4719753294456268 1117 75-79 0.4097625119102304 1117 80-84 0.6133384340811361 1117 85-89 0.6675972464637496 1117 90-94 0.5080483338835364 1117 95-99 0.032800743004976596 1117 100-104 0.631708681655148 1117 105-109 0.6101119793591607 1117 110-114 0.7888916873331837 1117 115-119 1.0102529173475219 1117 120-124 0.8305915256032819 1117 125-129 0.657525755322915 1117 130-134 0.6750472085904207 1117 135-139 0.5716518586384112 1117 140-144 0.6222881455857205 1117 145-149 -0.29194126749116833 1117 150-151 0.09814362489396444 2117 1 -30.953765341824457 2117 2 -10.523430855448531 2117 3 -1.864601293508393 2117 4 -0.23663438863093944 2117 5 0.06837182292357369 2117 6 1.2767377212538733 2117 7 0.09253548439841808 2117 8 0.28752432230933067 2117 9 0.29227856660602214 2117 10-14 0.41550555096038977 2117 15-19 0.40577155341557614 2117 20-24 0.3224432029963822 2117 25-29 0.4518161993387153 2117 30-34 0.5405991883472296 2117 35-39 0.3949675311542791 2117 40-44 0.25867415940880534 2117 45-49 0.5167741969285515 2117 50-54 0.39686820846615944 2117 55-59 0.5434957216300162 2117 60-64 0.6950874102368587 2117 65-69 0.577864412688271 2117 70-74 0.5448837257101644 2117 75-79 0.5941680151142563 2117 80-84 0.7605806065844476 2117 85-89 0.6756668622850199 2117 90-94 0.6042968314363861 2117 95-99 0.1674176366138198 2117 100-104 0.9175820698531538 2117 105-109 0.7737168875338014 2117 110-114 0.9579891272797667 2117 115-119 1.2066661397411806 2117 120-124 1.0639476414091291 2117 125-129 0.7322029655788356 2117 130-134 0.9101537788360545 2117 135-139 0.8029865019468687 2117 140-144 0.8510919512876924 2117 145-149 1.0830078399545329 2117 150-151 1.477418839683338 1116 1 0.3999039773621007 1116 2 0.2651909943961428 1116 3 -0.05343302456557808 1116 4 -0.27615496649745097 1116 5 0.09541617525093216 1116 6 1.2773724747512958 1116 7 -0.19767817057127957 1116 8 0.19508109306216426 1116 9 0.10811356169092079 1116 10-14 0.22675813259144917 1116 15-19 0.26199550936020444 1116 20-24 0.11526682361692053 1116 25-29 0.1027199115681583 1116 30-34 0.212622914850769 1116 35-39 0.127770863118009 1116 40-44 0.0570527422268583 1116 45-49 0.24843262708744618 1116 50-54 0.16250662053950293 1116 55-59 0.3163575070401876 1116 60-64 0.38069804477675007 1116 65-69 0.23204689583725724 1116 70-74 0.20188706094084807 1116 75-79 0.17091254012974844 1116 80-84 0.2534931052598637 1116 85-89 0.3187739754194112 1116 90-94 0.3747440405104854 1116 95-99 -0.41046860701508336 1116 100-104 0.6416198560105428 1116 105-109 0.4398321081729435 1116 110-114 0.5018938811667155 1116 115-119 0.9348491531428422 1116 120-124 0.7699222403793584 1116 125-129 0.48601544461060797 1116 130-134 0.5453777184458843 1116 135-139 0.6968716331341511 1116 140-144 0.47418110452108664 1116 145-149 0.5770630205776115 1116 150-151 0.7534700153490164 2118 1 0.8101412399165184 2118 2 0.3471600539623907 2118 3 -8.044392991735094E-4 2118 4 -0.08294564269291982 2118 5 0.028385139078665134 2118 6 1.26849075686453 2118 7 0.45834012111580336 2118 8 0.18291103894175365 2118 9 0.1424527125466568 2118 10-14 0.21338357538205344 2118 15-19 0.22202884888837815 2118 20-24 0.0976695223165649 2118 25-29 0.26223051883189186 2118 30-34 0.2115911916139126 2118 35-39 0.09071893342385806 2118 40-44 0.16014937617347869 2118 45-49 0.24762155295768196 2118 50-54 0.08019233916964907 2118 55-59 0.23496535647166894 2118 60-64 0.2930899869732144 2118 65-69 0.3054813740905331 2118 70-74 0.26172948661679385 2118 75-79 0.3278878790159041 2118 80-84 0.2353624841422075 2118 85-89 0.1261577798425364 2118 90-94 0.26728634848438304 2118 95-99 0.36031481558033107 2118 100-104 0.525335974433446 2118 105-109 0.2798713046452619 2118 110-114 0.5189066143885341 2118 115-119 0.8020970088817947 2118 120-124 0.43491415892277985 2118 125-129 0.23974648697771528 2118 130-134 0.3662471367499549 2118 135-139 0.4407582787383433 2118 140-144 0.21153106256509346 2118 145-149 0.13467496423830738 2118 150-151 1.032440206882356 1119 1 0.7774174538744703 1119 2 0.3425795457780225 1119 3 0.03237684965364451 1119 4 -0.028757573382598878 1119 5 0.10683456035118155 1119 6 1.2881633820271858 1119 7 0.1834301834403007 1119 8 0.272710367754847 1119 9 0.10197055379040876 1119 10-14 0.1774561731493307 1119 15-19 0.1698505049393404 1119 20-24 -0.0020841931823696314 1119 25-29 0.1938971170101027 1119 30-34 0.10830347159473774 1119 35-39 0.17971393376630118 1119 40-44 -0.04152174224307004 1119 45-49 0.18350704025307607 1119 50-54 0.19448368907719527 1119 55-59 0.31611541468686255 1119 60-64 0.3690316348055589 1119 65-69 0.22148986665953885 1119 70-74 0.17695063585314585 1119 75-79 0.24890096031170827 1119 80-84 0.18586286082874182 1119 85-89 0.25981547452097686 1119 90-94 0.33914334458053474 1119 95-99 0.3740793522558974 1119 100-104 0.5913774784400232 1119 105-109 0.3277924059396966 1119 110-114 0.44951739447208894 1119 115-119 0.8152007688982295 1119 120-124 0.5195993174735705 1119 125-129 0.2923445282077637 1119 130-134 0.4481248163609379 1119 135-139 0.4220872288102555 1119 140-144 0.48324724638811745 1119 145-149 0.5464407337061559 1119 150-151 1.0067474461125414 2119 1 0.8911794330268563 2119 2 0.3194816387609052 2119 3 0.048404658644969345 2119 4 0.03628385689850688 2119 5 0.07163627054933386 2119 6 1.2509141334363676 2119 7 0.4823536356287548 2119 8 0.2489232654992506 2119 9 0.09086504589262034 2119 10-14 0.17849924785173954 2119 15-19 0.15883832708603762 2119 20-24 0.09101238002635625 2119 25-29 0.21669277939213316 2119 30-34 0.2005129708684521 2119 35-39 0.17070637856399173 2119 40-44 -0.045875621706990444 2119 45-49 0.19438357162040631 2119 50-54 0.1916073404233316 2119 55-59 0.3477219960263369 2119 60-64 0.354783053118922 2119 65-69 0.2996660207235493 2119 70-74 0.31205077197468967 2119 75-79 0.19578019583852324 2119 80-84 0.37187782506256895 2119 85-89 0.5400682038035498 2119 90-94 0.38921593664529297 2119 95-99 0.4518583237617335 2119 100-104 0.7386175730856053 2119 105-109 0.4192909670710563 2119 110-114 0.5581741032542169 2119 115-119 1.0019393515897903 2119 120-124 0.6490332893905091 2119 125-129 0.4657715570125518 2119 130-134 0.4318229736955246 2119 135-139 0.23174652298809661 2119 140-144 0.30554041016334565 2119 145-149 0.35195272427814217 2119 150-151 0.37868439169133694 1118 1 0.9047650635809461 1118 2 0.4138893031160009 1118 3 0.058360756948033554 1118 4 0.10807583260403675 1118 5 0.1273838192875374 1118 6 1.3507879824340492 1118 7 0.441506687363578 1118 8 0.37416688475571647 1118 9 0.11233690804789376 1118 10-14 0.22889463580553127 1118 15-19 0.20385163106546145 1118 20-24 0.13682571672753596 1118 25-29 0.2784238897505844 1118 30-34 0.19867552913615327 1118 35-39 0.09562780328097631 1118 40-44 0.14214979219459423 1118 45-49 0.255049215492825 1118 50-54 0.1468638474613826 1118 55-59 0.3262750339835776 1118 60-64 0.4002980907776532 1118 65-69 0.12514414593881895 1118 70-74 0.18202848386244597 1118 75-79 -0.01934213762493897 1118 80-84 -0.21229058293761227 1118 85-89 0.32629004586651433 1118 90-94 0.2778060879690969 1118 95-99 0.18615331331373852 1118 100-104 0.3687297742842546 1118 105-109 0.36157717054300775 1118 110-114 0.3750452570120544 1118 115-119 0.8839486256259335 1118 120-124 0.49772090276854186 1118 125-129 0.2720863547116892 1118 130-134 0.3749668317634445 1118 135-139 0.48889077429122096 1118 140-144 0.44418560006437247 1118 145-149 0.5764258034533185 1118 150-151 0.9016274773534576 2112 1 0.9672086009350451 2112 2 0.34153431650829447 2112 3 0.06572056362570322 2112 4 0.08378967919303903 2112 5 0.04948052085882182 2112 6 1.2414701666473107 2112 7 0.48413652619429826 2112 8 0.42447401990239797 2112 9 0.048423177198287704 2112 10-14 0.11297866376771282 2112 15-19 0.10152187045265038 2112 20-24 0.05683535686746666 2112 25-29 0.20766335053772167 2112 30-34 0.2905771621873967 2112 35-39 0.028722454157829702 2112 40-44 0.12992304975373514 2112 45-49 0.140355037386918 2112 50-54 0.11188511205147478 2112 55-59 0.14543430699003324 2112 60-64 0.3368134760368946 2112 65-69 0.09930846270111715 2112 70-74 0.12639769689222646 2112 75-79 0.3501258940547558 2112 80-84 0.1539441054030064 2112 85-89 0.313698118184881 2112 90-94 0.2580217211588831 2112 95-99 0.158737650106751 2112 100-104 0.49146513512565804 2112 105-109 0.21695562540752888 2112 110-114 0.3624347489709123 2112 115-119 0.7547686219892356 2112 120-124 0.4261615163316961 2112 125-129 0.26148173177392664 2112 130-134 0.4302015504098975 2112 135-139 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2108 35-39 -1.0357289662243758 2108 40-44 -0.650784297555532 2108 45-49 -0.36856915523638634 2108 50-54 -0.41111836824488535 2108 55-59 -0.45658128932085873 2108 60-64 -0.7577829265604379 2108 65-69 -0.6481140769066727 2108 70-74 -0.6575033238886974 2108 75-79 -0.9685453502715404 2108 80-84 -0.7975277068539768 2108 85-89 -1.3369812442915645 2108 90-94 -1.2217668340094932 2108 95-99 -1.1630881451258475 2108 100-104 -0.9052790222481306 2108 105-109 -0.8560844179322658 2108 110-114 -1.2760686517079876 2108 115-119 -1.2779760939049183 2108 120-124 -1.5173782175782193 2108 125-129 -1.0234723525805052 2108 130-134 -1.2791302488076965 2108 135-139 -1.5466905617618139 2108 140-144 -1.6086557436378186 2108 145-149 -1.722485785021945 2108 150-151 -2.1148461011779 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 2.0 16 5.0 17 18.0 18 55.0 19 120.0 20 264.0 21 633.0 22 1228.0 23 2148.0 24 2895.0 25 3630.0 26 4660.0 27 6034.0 28 8075.0 29 10886.0 30 14359.0 31 19601.0 32 28945.0 33 35613.0 34 41484.0 35 64323.0 36 129807.0 37 496299.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.1087098470650106 0.08000291775347101 99.6271628729989 0.18412436218262077 2 53.99812188032382 0.0451162000450014 27.14824287898756 18.808519040643613 3 0.06887969472519298 0.1157178871383242 0.09172479347571531 99.72367762466077 4 0.362651592728141 24.569272308985127 0.1529129222899284 74.9151631759968 5 72.37132125030422 27.246855641935795 0.1779392113734152 0.20388389638657123 6 28.148950043853404 29.909629840520545 23.71011291677955 18.231307198846494 7 0.6683626378167892 45.35544218468024 24.559399552741183 29.416795624761793 8 72.37407643809323 0.2992822735809635 26.95101735309109 0.37562393523471904 9 53.72421029429998 18.670070854245974 0.6640002571508603 26.94171859430319 10-14 17.88667912623811 43.65314940924182 23.1647923736402 15.295379090879868 15-19 31.31286994136042 21.57068663871682 20.439475412244974 26.676968007677793 20-24 34.07744832874901 24.78897557526025 9.574047967819405 31.559528128171337 25-29 23.945750352434438 44.16432858369571 6.779047715260527 25.11087334860932 30-34 22.463069003678175 18.15262362757208 35.49540572436183 23.88890164438791 35-39 41.74323027400342 21.289450845153855 19.980621845883977 16.98669703495874 40-44 12.899031551492163 32.15786307635084 28.18215005671095 26.760955315446044 45-49 15.526929664647726 26.901676531769613 22.576031703027493 34.99536210055517 50-54 28.85120149147499 25.575076571260634 16.511197542372493 29.06252439489188 55-59 19.160540200485833 42.30032924494079 32.82815434561994 5.710976208953442 60-64 34.91706884755087 20.65151007250736 22.351552777918087 22.079868302023687 65-69 18.273094213646445 34.93761795647722 25.56068071506307 21.228607114813265 70-74 24.129865776434876 14.537656529106263 30.417893107897747 30.914584586561112 75-79 30.20994530952239 18.723291898370306 26.573855104674177 24.49290768743313 80-84 21.161931570319283 21.56818400980847 33.24044523834671 24.029439181525547 85-89 30.080933641302103 21.42284785393831 35.83247999044868 12.663738514310904 90-94 23.45698003866447 36.75663885457659 29.003196017835247 10.783185088923686 95-99 15.697682427871479 31.370705925031338 33.583718676958824 19.347892970138357 100-104 9.949304544682258 38.76615802838762 28.553916729041056 22.730620697889066 105-109 20.50987046025412 27.242516221168106 32.178435145175435 20.069178173402335 110-114 40.540338245220894 24.774671558655655 12.77468074261495 21.910309453508503 115-119 9.778299222577845 43.36908954819512 15.229415303231375 31.623195925995656 120-124 4.590464295062245 22.33419509484734 46.50160030490745 26.57374030518297 125-129 20.415620077971813 30.313471490694354 17.138599721726035 32.1323087096078 130-134 16.27801681582947 33.564547161926974 43.70907972135868 6.448356300884875 135-139 32.25638399970611 1.6811237492595432 46.27360851536706 19.788883735667284 140-144 13.796625813354396 39.34722713308935 16.998521382553232 29.857625671003024 145-149 26.104899183086822 19.549618636090205 33.54142654439756 20.80405563642542 150-151 38.56346722214346 19.99268726821252 25.502434611607267 15.941410898036757 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.0 20 0.5 21 0.0 22 0.0 23 0.0 24 0.0 25 0.5 26 1.0 27 1.0 28 3.0 29 4.0 30 8.0 31 15.5 32 35.0 33 133.0 34 534.5 35 4849.0 36 115037.5 37 122526.0 38 12905.0 39 1271.5 40 443.0 41 311.0 42 286.5 43 335.0 44 595.5 45 1357.0 46 7601.5 47 72081.5 48 74778.0 49 18154.5 50 104808.0 51 107498.0 52 17408.5 53 12536.0 54 8923.0 55 2916.5 56 864.5 57 406.0 58 360.5 59 585.5 60 1440.0 61 7312.0 62 78958.5 63 82049.5 64 10286.0 65 1115.5 66 220.0 67 62.0 68 28.5 69 13.5 70 13.5 71 6.5 72 1.0 73 0.5 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.4239912568707496 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 5.739974560432748E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 871084.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 1.3533359879654228 #Duplication Level Percentage of deduplicated Percentage of total 1 73.15150675316823 0.9899856666295824 2 8.88817868237641 0.24057384156654177 3 3.604313045863102 0.14633539670579412 4 2.0753764557956704 0.11234726584817645 5 1.6433286246324317 0.11119878838843958 6 1.0825258237150521 0.0879012693081296 7 0.6169128373199212 0.058442324092803526 8 0.5819989241085762 0.06301120711626346 9 0.43630377449973035 0.05314190397440722 >10 4.50716875899219 1.302109095606133 >50 1.496660666459941 1.4891363820923642 >100 1.5992888264457654 4.169720760240907 >500 0.11118050665130988 1.0892478230896347 >1k 0.12828519998228063 5.119383749131615 >5k 0.0342093866619415 3.016236135343309 >10k+ 0.04276173332742688 81.9512283908659 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG 221666 25.44714401825771 No Hit TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC 195421 22.43423137148656 No Hit TGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG 152015 17.451244656083684 No Hit TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG 128233 14.72108315615945 No Hit TGCAGTTGGTCGACAGTCCTCGGCACGTGAGAAAACGAGAAAGTGAGCTG 10716 1.2301913477919466 No Hit TGCAGTTGGTCGACAGTCCTCGGTACGTGAGAAAACGAGAAAGTGAGCTG 9371 1.0757860321163057 No Hit NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG 5933 0.6811053813409499 No Hit TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC 5517 0.6333487929981494 No Hit NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC 5239 0.6014345344421433 No Hit TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG 4907 0.5633211033608699 No Hit TGCAGTTGGTCGACAGTCCTCGGTCCGCAACGGTGTGGACATGGCAGAGG 4214 0.483765055953272 No Hit NGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG 4114 0.47228510683240654 No Hit TGCAGGTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG 3950 0.4534579902741871 No Hit TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG 3865 0.44370003352145143 No Hit TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG 3731 0.42831690169949166 No Hit NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG 3565 0.4092601861588549 No Hit TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG 3226 0.37034315863912093 No Hit TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC 3220 0.369654361691869 No Hit TGCAGATGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG 2234 0.2564620633601352 No Hit TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC 1760 0.20204710452723273 No Hit TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG 1590 0.1825311910217614 No Hit TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC 1575 0.18080919865363157 No Hit TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA 1216 0.13959618130972443 No Hit TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG 1064 0.12214665864600889 No Hit TGCAGCTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG 947 0.10871511817459624 No Hit TTCCATATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC 939 0.10779672224492702 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.008495162349440467 6 0.0 0.0 0.008495162349440467 7 0.0 0.0 0.008495162349440467 8 0.0 0.0 0.008495162349440467 9 0.0 0.0 0.008495162349440467 10-11 0.0 0.0 0.008495162349440467 12-13 0.0 0.0 0.008495162349440467 14-15 0.0 0.0 0.008495162349440467 16-17 0.0 0.0 0.008495162349440467 18-19 0.0 0.0 0.008495162349440467 20-21 0.0 0.0 0.008724761331857777 22-23 0.0 0.0 0.010848551919217894 24-25 0.0 0.0 0.011135550647239532 26-27 0.0 0.0 0.011250350138448186 28-29 0.0 0.0 0.011307749884052514 30-31 0.0 0.0 0.011652148357678478 32-33 0.0 0.0 0.012570544287347718 34-35 0.0 0.0 0.01320194148899532 36-37 0.0 0.0 0.01366113945382994 38-39 0.0 0.0 0.013948138181851578 40-41 0.0 0.0 0.01480913436591649 42-43 0.0 0.0 0.028872072038976723 44-45 0.0 0.0 0.04230361251038935 46-47 0.0 0.0 0.08925660441472924 48-49 0.0 0.0 3.05894724274582 50-51 0.0 0.0 3.114510196490809 52-53 0.0 0.0 3.1245551519715664 54-55 0.0 0.0 3.143382268529786 56-57 0.0 0.0 3.2353366609879184 58-59 0.0 0.0 3.2808546592521504 60-61 0.0 0.0 3.288718424399943 62-63 0.0 0.0 3.2937122022675194 64-65 0.0 0.0 3.2937122022675194 66-67 0.0 0.0 3.293941801249937 68-69 0.0 0.0 3.2958359928548795 70-71 0.0 0.0 3.296008192091693 72-73 0.0 0.0 3.2961803913285057 74-75 0.0 0.0 3.2964673900565273 76-77 0.0 0.0 3.3014611679241037 78-79 0.0 0.0 3.304790353169155 80-81 0.0 0.0 3.3066845447740976 82-83 0.0 0.0 3.3067993442653063 84-85 0.0 0.0 3.3067993442653063 86-87 0.0 0.0 3.307143742738932 88-89 0.0 0.0 3.3072011424845362 90-91 0.0 0.0 3.307258542230141 92-93 0.0 0.0 3.307258542230141 94-95 0.0 0.0 3.3074881412125583 96-97 0.0 0.0 3.3074881412125583 98-99 0.0 0.0 3.3074881412125583 100-101 0.0 0.0 3.3076029407037666 102-103 0.0 0.0 3.322584274306496 104-105 0.0 0.0 3.3374508084180174 106-107 0.0 0.0 3.3384266040932906 108-109 0.0 0.0 3.3385414035844994 110-111 0.0 0.0 3.3386562030757077 112-113 0.0 0.0 3.3387136028213122 114-115 0.0 0.0 3.3387136028213122 116-117 0.0 0.0 3.3387136028213122 118-119 0.0 0.0 3.338771002566917 120-121 0.0 0.0 3.3395745990053776 122-123 0.0 0.0 3.348299360337235 124-125 0.0 0.0 3.351628545582286 126-127 0.0 0.0 3.351743345073495 128-129 0.0 0.0 3.353752336169646 130-131 0.0 0.0 3.3538097359152506 132-133 0.0 0.0 3.3539819351520634 134-135 0.0 0.0 3.3539819351520634 136-137 0.0 0.0 3.3541541343888763 138-139 0.0 0.0 3.354441133116898 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCCGCG 55 0.0 148.57481 1 TGCCAGT 10 0.006592714 148.5748 1 TGCGGTT 25 3.2086973E-8 148.5748 1 TTTCAGA 15 1.1162167E-4 148.5748 1 CCCCGCA 35 9.094947E-12 148.5748 1 ACCCGCA 15 1.1162167E-4 148.5748 1 TCCCCAG 10 0.006592714 148.5748 1 TACAGTT 10 0.006592714 148.5748 1 TGCAGGT 510 0.0 148.5748 1 AGCAGTT 10 0.006592714 148.5748 1 GCCCGCA 10 0.006592714 148.5748 1 TGCGTTG 25 3.2086973E-8 148.5748 1 TGCCGAG 50 0.0 148.5748 1 TCCCGTA 90 0.0 148.57478 1 TCCCGCA 14495 0.0 148.47229 1 TGCAGTT 19670 0.0 148.38596 1 TTCCAGA 22645 0.0 148.21394 1 TCCCGGA 355 0.0 146.4822 1 GGCGGAA 445 0.0 144.97578 5 GTCGGAA 65 0.0 144.97578 5 >>END_MODULE