##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AG1W8 l01n01 mis09.3410000000d1ea.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 667331 Sequences flagged as poor quality 0 Sequence length 151 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.064722004522494 34.0 34.0 34.0 34.0 34.0 2 33.540400490910805 34.0 34.0 34.0 34.0 34.0 3 33.86575477536635 34.0 34.0 34.0 34.0 34.0 4 33.785259488919294 34.0 34.0 34.0 34.0 34.0 5 33.8123929504249 34.0 34.0 34.0 34.0 34.0 6 36.46969494898334 38.0 38.0 38.0 34.0 38.0 7 37.18975740674418 38.0 38.0 38.0 36.0 38.0 8 37.27015379174652 38.0 38.0 38.0 37.0 38.0 9 37.460251359520235 38.0 38.0 38.0 37.0 38.0 10-14 37.30665501827429 38.0 38.0 38.0 36.4 38.0 15-19 37.252986598854235 38.0 38.0 38.0 36.4 38.0 20-24 37.40471130518439 38.0 38.0 38.0 36.8 38.0 25-29 37.13596551036892 38.0 38.0 38.0 36.2 38.0 30-34 36.868412227215586 38.0 37.8 38.0 35.4 38.0 35-39 36.96641726519523 38.0 38.0 38.0 35.6 38.0 40-44 37.04196178508117 38.0 38.0 38.0 36.2 38.0 45-49 36.87411254684706 38.0 38.0 38.0 35.4 38.0 50-54 36.9123712220772 38.0 38.0 38.0 35.6 38.0 55-59 36.57189730433623 38.0 37.6 38.0 34.4 38.0 60-64 36.57248411957484 38.0 37.4 38.0 34.2 38.0 65-69 36.57310120464957 38.0 37.2 38.0 34.2 38.0 70-74 36.59064632094118 38.0 37.8 38.0 34.4 38.0 75-79 36.25259968441448 38.0 37.2 38.0 33.2 38.0 80-84 36.251387692164755 38.0 37.0 38.0 33.6 38.0 85-89 36.16639748490629 38.0 37.0 38.0 33.0 38.0 90-94 36.236001324679954 38.0 37.0 38.0 33.8 38.0 95-99 35.91702678281093 38.0 36.8 38.0 31.8 38.0 100-104 35.73106059811398 38.0 36.8 38.0 30.8 38.0 105-109 35.79151605425194 38.0 37.0 38.0 31.2 38.0 110-114 35.831243266085345 38.0 36.8 38.0 31.6 38.0 115-119 35.262311206882345 38.0 36.2 38.0 28.4 38.0 120-124 35.14448212356386 38.0 36.2 38.0 27.6 38.0 125-129 35.06160930632625 38.0 36.4 38.0 27.2 38.0 130-134 34.525176861257755 38.0 35.0 38.0 24.8 38.0 135-139 34.39918091621699 38.0 35.2 38.0 24.0 38.0 140-144 34.74952070261984 38.0 36.0 38.0 25.8 38.0 145-149 34.15076536231645 37.8 34.8 38.0 23.2 38.0 150-151 31.9600273327629 35.5 29.5 37.5 16.5 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8203018907362036 1101 2 0.3686099767080435 1101 3 0.04968238083018406 1101 4 0.04922201739691445 1101 5 0.085339323524245 1101 6 1.1905658351087638 1101 7 0.384327801124428 1101 8 0.29138713695351015 1101 9 0.14634555523180381 1101 10-14 0.18877024909600237 1101 15-19 0.16419883962114312 1101 20-24 0.07284162817772 1101 25-29 0.164821485393837 1101 30-34 0.14778346303670276 1101 35-39 -0.014814844538506122 1101 40-44 0.12487683570919472 1101 45-49 0.14347497937740883 1101 50-54 0.13193934816681718 1101 55-59 0.2134475825727975 1101 60-64 0.26700311443959635 1101 65-69 0.1087225905568161 1101 70-74 0.10989802848710895 1101 75-79 0.2071560612390826 1101 80-84 0.28782562576419934 1101 85-89 0.32539770593584905 1101 90-94 0.2469096790153671 1101 95-99 0.31134660487119703 1101 100-104 0.4390554259091033 1101 105-109 0.25805902834248684 1101 110-114 0.18996023888704627 1101 115-119 0.441119342608431 1101 120-124 0.3368111412203092 1101 125-129 0.1698323755408211 1101 130-134 0.2891404390210468 1101 135-139 0.35409072145580467 1101 140-144 0.28200232827764893 1101 145-149 0.23678832912776215 1101 150-151 -0.15448849446596213 1102 1 0.8771622857985619 1102 2 0.32307365583844927 1102 3 0.05348687495295934 1102 4 0.06594423036142416 1102 5 0.10839765995535089 1102 6 1.1429154435073627 1102 7 0.397745116187302 1102 8 0.08722725334436632 1102 9 0.12810212144512434 1102 10-14 0.23184890265374491 1102 15-19 0.2117916767124015 1102 20-24 0.1408304249167287 1102 25-29 0.22433688667310747 1102 30-34 0.08567945473039629 1102 35-39 -0.01945125129122971 1102 40-44 0.1485907680025278 1102 45-49 0.09056424655083362 1102 50-54 0.021641256823066612 1102 55-59 0.16818935637972743 1102 60-64 0.26953621789672155 1102 65-69 0.21219457599513447 1102 70-74 0.13850300458083353 1102 75-79 0.1602082448495068 1102 80-84 0.33508531325217916 1102 85-89 0.17518575408166726 1102 90-94 0.24386896785183865 1102 95-99 0.37596468134562855 1102 100-104 0.5801652409399267 1102 105-109 0.24906732183202962 1102 110-114 0.1563204609013269 1102 115-119 0.48121541633973663 1102 120-124 0.30578791432222374 1102 125-129 0.07626213724719832 1102 130-134 0.3082707762725292 1102 135-139 0.3600801078401261 1102 140-144 0.3163310299042976 1102 145-149 0.38199303859926204 1102 150-151 0.6896379652266553 1103 1 0.8870439721841663 1103 2 0.35051042919673137 1103 3 0.04911676997046044 1103 4 0.08146183640143789 1103 5 0.0734614954699424 1103 6 1.29294140071962 1103 7 0.5008436382284955 1103 8 0.2631065939670876 1103 9 0.11693379052591979 1103 10-14 0.17813676493311448 1103 15-19 0.2957599255940053 1103 20-24 0.13607692229535928 1103 25-29 0.3450251053033355 1103 30-34 0.4092088024032208 1103 35-39 0.2220629835157908 1103 40-44 0.31854199408022765 1103 45-49 0.3401944363909877 1103 50-54 0.29347780970528703 1103 55-59 0.45292722058184864 1103 60-64 0.47322483389661585 1103 65-69 0.4515958937242317 1103 70-74 0.404128797717874 1103 75-79 0.41609271282279536 1103 80-84 0.5267396529135127 1103 85-89 0.5421397873838103 1103 90-94 0.514782982182787 1103 95-99 0.6405321252331859 1103 100-104 0.7091911725154318 1103 105-109 0.5456155894284578 1103 110-114 0.5460688361721111 1103 115-119 1.0403274874048165 1103 120-124 0.8695034489126101 1103 125-129 0.601959072373397 1103 130-134 0.7847286743151685 1103 135-139 0.727733255392458 1103 140-144 0.7203507445672415 1103 145-149 0.8324896865938243 1103 150-151 0.7666087906019108 2116 1 0.712277166690761 2116 2 0.2806589773408419 2116 3 -0.07355128496362795 2116 4 -0.16584183143108788 2116 5 0.03329716714690534 2116 6 1.1334030636221684 2116 7 0.08009766733709967 2116 8 0.19694799522579132 2116 9 0.07402646465727969 2116 10-14 0.18514153605432426 2116 15-19 0.23922908937689868 2116 20-24 0.145362273470532 2116 25-29 0.24534348894702163 2116 30-34 0.21655644821763786 2116 35-39 0.10583812655676184 2116 40-44 0.07347257188366285 2116 45-49 0.20786757487265106 2116 50-54 0.18087795903448978 2116 55-59 0.3834952937849323 2116 60-64 0.4119972101109042 2116 65-69 0.30771911944698616 2116 70-74 0.23597159695216874 2116 75-79 0.35911415374376077 2116 80-84 0.4084395435642847 2116 85-89 0.429450445094254 2116 90-94 0.4772111307388798 2116 95-99 0.5321781620518422 2116 100-104 0.7177116748334811 2116 105-109 0.4267831638084374 2116 110-114 0.37984159630016023 2116 115-119 0.7676659854812797 2116 120-124 0.5136389678183804 2116 125-129 0.3854873853504728 2116 130-134 0.6837060075178769 2116 135-139 0.6159406826672793 2116 140-144 0.5176994891692814 2116 145-149 0.7882009331485378 2116 150-151 1.4671438207457648 1117 1 0.87578844491631 1117 2 0.3614992561275443 1117 3 0.04219931050375436 1117 4 -0.02876270724464547 1117 5 0.08123445746707603 1117 6 1.0821308893764865 1117 7 0.3961309015480552 1117 8 0.24391061173680129 1117 9 0.18689302531497987 1117 10-14 0.09727554187370657 1117 15-19 0.16181013225455132 1117 20-24 0.0986963576636839 1117 25-29 0.26879570693807153 1117 30-34 0.26221723374141703 1117 35-39 0.1339704449408714 1117 40-44 0.2273941261368364 1117 45-49 0.30922112188557804 1117 50-54 0.17322378729730303 1117 55-59 0.4163387688352813 1117 60-64 0.47445397458039196 1117 65-69 0.3278069967040622 1117 70-74 0.2791184904584796 1117 75-79 0.22717224363634614 1117 80-84 0.43614760393341356 1117 85-89 0.4606503368638073 1117 90-94 0.35985533914379886 1117 95-99 -0.18899301932612644 1117 100-104 0.4667520626503574 1117 105-109 0.4232746046728977 1117 110-114 0.4687205847986817 1117 115-119 0.8555147187718717 1117 120-124 0.7017177574501119 1117 125-129 0.47495431041955527 1117 130-134 0.6468056901574215 1117 135-139 0.426348732773171 1117 140-144 0.4767407300340025 1117 145-149 -0.6154068594435458 1117 150-151 0.009254561018014584 2117 1 -30.96589720428642 2117 2 -10.543372717482601 2117 3 -1.854654789869869 2117 4 -0.33894925623417294 2117 5 0.040051347208923005 2117 6 1.2157032908593095 2117 7 -0.08592367482299323 2117 8 0.20677508372091324 2117 9 0.23637327813028008 2117 10-14 0.4141581379263144 2117 15-19 0.4299435340319633 2117 20-24 0.3268421843738025 2117 25-29 0.43947038661688254 2117 30-34 0.4616553409516584 2117 35-39 0.28455537927247576 2117 40-44 0.2468035626690579 2117 45-49 0.49601161010377126 2117 50-54 0.4161299090558046 2117 55-59 0.5552234123767974 2117 60-64 0.6393913103364923 2117 65-69 0.4328325319824131 2117 70-74 0.40206261617779404 2117 75-79 0.5478823606232126 2117 80-84 0.6285319368516156 2117 85-89 0.652123362218255 2117 90-94 0.5407696508579392 2117 95-99 -0.030717751650492175 2117 100-104 0.8671641854180265 2117 105-109 0.7814276646771603 2117 110-114 0.7693271023467076 2117 115-119 1.1897602832750636 2117 120-124 1.1431843661726546 2117 125-129 1.0459734465659025 2117 130-134 1.093621728237217 2117 135-139 0.9573517106737199 2117 140-144 1.0589064308031269 2117 145-149 1.2682822080740053 2117 150-151 1.5738383257726234 1116 1 0.13871997864753638 1116 2 0.1757049385069962 1116 3 -0.1282347960212249 1116 4 -0.27995424691359716 1116 5 0.028215601851066197 1116 6 1.1561088612028954 1116 7 -0.12943627799101165 1116 8 0.17478766481290364 1116 9 0.10964564170976843 1116 10-14 0.20457541979081384 1116 15-19 0.22289304877089222 1116 20-24 0.11749639068064965 1116 25-29 0.09904064489146691 1116 30-34 0.27434224821961806 1116 35-39 0.30834009382941474 1116 40-44 0.14898362674192356 1116 45-49 0.27862318198444314 1116 50-54 0.20578710198758188 1116 55-59 0.38613337136028036 1116 60-64 0.5158328189599928 1116 65-69 0.37297333963584123 1116 70-74 0.3512001169167718 1116 75-79 0.29622968916167736 1116 80-84 0.4109428787796787 1116 85-89 0.43085778458584656 1116 90-94 0.4238995978530298 1116 95-99 -0.5872114345384247 1116 100-104 0.6517483815250102 1116 105-109 0.46978499103794746 1116 110-114 0.5785575768466416 1116 115-119 0.9295014773712538 1116 120-124 0.5411571879607138 1116 125-129 0.4221538593652099 1116 130-134 0.5540941852095429 1116 135-139 0.6033333119866029 1116 140-144 0.4455400308198918 1116 145-149 0.5164142391959672 1116 150-151 0.5286095919115077 2118 1 0.8118805734913579 2118 2 0.36284912535896297 2118 3 0.05379591435548292 2118 4 -0.0443443653851503 2118 5 0.04628741682527959 2118 6 1.1934541958402605 2118 7 0.39507567706837676 2118 8 0.1720064268865258 2118 9 0.09056464405873044 2118 10-14 0.18815791662092352 2118 15-19 0.06397081782449732 2118 20-24 0.031688772318979375 2118 25-29 0.16541528157586782 2118 30-34 0.09450812544764631 2118 35-39 0.008560690601733256 2118 40-44 0.09528999190666099 2118 45-49 0.18642750615346415 2118 50-54 0.0032199013101177343 2118 55-59 0.07695876275064961 2118 60-64 0.12506652887556413 2118 65-69 0.15324321154803044 2118 70-74 0.21969433240105474 2118 75-79 0.25213932779097803 2118 80-84 0.21468928498079265 2118 85-89 -0.007199127151181983 2118 90-94 0.22587263689989356 2118 95-99 0.3390471904149237 2118 100-104 0.4585048218290808 2118 105-109 0.1353850791979454 2118 110-114 0.3396121120683304 2118 115-119 0.6379068506704542 2118 120-124 0.3057568832052624 2118 125-129 0.37340899137233663 2118 130-134 0.33356418883695227 2118 135-139 0.5026134042922195 2118 140-144 0.4181672708151396 2118 145-149 0.2732252219372029 2118 150-151 1.1077889430565726 1119 1 0.7626824391868396 1119 2 0.27913775441354716 1119 3 0.03142150900235663 1119 4 -0.058708503862767714 1119 5 0.03708188634315235 1119 6 1.226988052069153 1119 7 0.18205930802363923 1119 8 0.31095323959220167 1119 9 0.1638287527838358 1119 10-14 0.2726462089106363 1119 15-19 0.159030997281846 1119 20-24 0.046582582957469754 1119 25-29 0.21977563806388645 1119 30-34 0.18462577372132216 1119 35-39 0.22476451531619546 1119 40-44 0.0342495833485188 1119 45-49 0.2652239040988107 1119 50-54 0.1790913885291232 1119 55-59 0.3194965939409613 1119 60-64 0.21311331195780525 1119 65-69 0.1927964979098249 1119 70-74 0.1244937159238475 1119 75-79 0.3431683322951571 1119 80-84 0.19765639135215451 1119 85-89 0.3168106337380152 1119 90-94 0.32146616724519106 1119 95-99 0.34061174658940985 1119 100-104 0.5157864748554175 1119 105-109 0.44589868758389883 1119 110-114 0.36227152196178025 1119 115-119 0.6808965199012604 1119 120-124 0.5624343214429786 1119 125-129 0.30362715279616737 1119 130-134 0.39728425964922565 1119 135-139 0.4818589296370277 1119 140-144 0.4388193865027219 1119 145-149 0.6169499266548186 1119 150-151 1.1137907540229257 2119 1 0.9054744519457927 2119 2 0.34962161213144327 2119 3 0.07982168141710844 2119 4 0.06073060489566018 2119 5 0.038607697990180156 2119 6 1.254854897347208 2119 7 0.43792240783011493 2119 8 0.16963336342448798 2119 9 0.028873298170843498 2119 10-14 0.14247392180014629 2119 15-19 0.04433826897547988 2119 20-24 0.018051737964810854 2119 25-29 0.03795590312046926 2119 30-34 0.09573464473525917 2119 35-39 0.06619144703727642 2119 40-44 -0.22681999686999887 2119 45-49 0.06416139982361813 2119 50-54 0.06025704055176817 2119 55-59 0.1611447061663327 2119 60-64 0.17039529877272486 2119 65-69 0.1328752365983732 2119 70-74 0.1353136253111913 2119 75-79 0.011540076567555957 2119 80-84 0.20821015101161322 2119 85-89 0.29797121281331584 2119 90-94 0.27391870818634345 2119 95-99 0.2774175505648344 2119 100-104 0.5310772545608842 2119 105-109 0.3226437405601956 2119 110-114 0.3466446840411379 2119 115-119 0.752989058315741 2119 120-124 0.38786104631871865 2119 125-129 0.20055402675110656 2119 130-134 0.2701716470911535 2119 135-139 -0.028856738864504905 2119 140-144 0.03872624064370456 2119 145-149 -0.002834240709240987 2119 150-151 0.06533985213470217 1118 1 0.9100675145669399 1118 2 0.4274291408761286 1118 3 0.08406553448177334 1118 4 0.03017084676328352 1118 5 0.10498167287344984 1118 6 1.232964823369997 1118 7 0.37342155159213064 1118 8 0.3159628352677757 1118 9 0.05505271971917125 1118 10-14 0.08249390173236293 1118 15-19 0.12008593196974715 1118 20-24 0.0583597451861948 1118 25-29 0.17521188784435537 1118 30-34 0.07843844914275877 1118 35-39 -0.023850150629392886 1118 40-44 -0.004107758722255994 1118 45-49 0.10036994413118805 1118 50-54 0.12967952387410975 1118 55-59 0.20255368044405486 1118 60-64 0.1702624548138587 1118 65-69 -0.02087875044604459 1118 70-74 0.09236072436183207 1118 75-79 -0.1381042818940159 1118 80-84 -0.46781826015079275 1118 85-89 0.014576017409702047 1118 90-94 0.11759606993167182 1118 95-99 -0.00948059633410736 1118 100-104 0.2052983729225204 1118 105-109 0.2091131723306745 1118 110-114 0.08389878623373193 1118 115-119 0.6693074671500838 1118 120-124 0.46264278250203716 1118 125-129 0.2998521563307577 1118 130-134 0.4720158375054311 1118 135-139 0.46704950856661753 1118 140-144 0.4336841943070695 1118 145-149 0.6378595599269232 1118 150-151 0.6481342062978293 2112 1 0.9198798412929321 2112 2 0.3535441036980913 2112 3 0.05299715243646119 2112 4 -0.024048971393987983 2112 5 0.035002131240155165 2112 6 1.1776527621365744 2112 7 0.42567454511920033 2112 8 0.2857375510514757 2112 9 0.007517441297089533 2112 10-14 0.10390979750185636 2112 15-19 0.07401543385483933 2112 20-24 0.06387000252326658 2112 25-29 0.18584139887183682 2112 30-34 0.2849827046646709 2112 35-39 0.02353636719190888 2112 40-44 0.05730960225136528 2112 45-49 0.08766946999839575 2112 50-54 0.08054541713096341 2112 55-59 0.18754254307632579 2112 60-64 0.22864339214490315 2112 65-69 0.1498928490543321 2112 70-74 0.1854146880719867 2112 75-79 0.30588084325682274 2112 80-84 0.024727059763208104 2112 85-89 0.1757687541143369 2112 90-94 0.17732214941475632 2112 95-99 0.19245625821168488 2112 100-104 0.3691455509212176 2112 105-109 0.25339660577620293 2112 110-114 0.2794409329515233 2112 115-119 0.6938765790729136 2112 120-124 0.38463610189776887 2112 125-129 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5299.0 28 6740.0 29 8653.0 30 11252.0 31 15018.0 32 21622.0 33 26654.0 34 31493.0 35 49384.0 36 99410.0 37 378014.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.1066423614147989 0.06904901098799927 99.63956416264264 0.18474446495455807 2 56.40529212639605 0.0457044555100842 29.788216042713433 13.760787375380435 3 0.06233788030227878 0.09695338595089993 0.09035995630354351 99.75034877744328 4 0.3206804419396072 22.914715486018185 0.14130918539675213 76.62329488664545 5 69.817077282488 29.87003451061018 0.15539514873428628 0.15749305816753603 6 30.582274763198473 33.71250548828093 22.238888947164153 13.466330801356449 7 0.630721486039162 42.99215831423986 22.9406396525862 33.43648054713478 8 69.78890535581293 0.2631377831990422 29.557296154382158 0.39066070660586727 9 56.11428211786954 13.731416643314937 0.595356727021523 29.558944511794 10-14 16.094232097714627 46.828845055901795 21.898128514934868 15.178794331448712 15-19 30.488528181666968 18.741164429645856 18.54276213752995 32.22754525115722 20-24 29.117604307307765 31.34855116876033 5.094443387164691 34.43940113676721 25-29 21.76080535746129 42.67789148113905 11.629311391198671 23.931991770200995 30-34 17.5271941510285 18.424380105225143 39.85902048608562 24.18940525766074 35-39 37.47942175621993 26.237833998420573 22.080077203067145 14.202667042292354 40-44 10.9248334035134 32.779505223045234 28.590759308349227 27.704902065092135 45-49 12.794550230695112 31.315823781601633 19.79212714529971 36.097498842403546 50-54 25.215372880924157 26.236725103434427 18.526578264759166 30.021323750882246 55-59 20.74616644513742 38.189654009779254 34.493227498797445 6.570952046285876 60-64 28.44864092931394 21.828298100942412 26.789704059904306 22.933356909839343 65-69 18.109843540911484 37.9790838429505 23.535966409472962 20.37510620666506 70-74 22.721617907754922 15.161591474096065 28.03478333840328 34.08200727974574 75-79 31.341298396148236 21.572083418873092 21.85314334265904 25.23347484231963 80-84 18.399954445395164 23.322429199302892 31.766784399346054 26.510831955955886 85-89 28.22632246965898 21.94895786348903 30.802375432881135 19.02234423397085 90-94 21.541064329395756 40.6145975535379 26.16018137925557 11.684156737810772 95-99 22.13893854773718 29.906747925692045 30.010894143985517 17.943419382585255 100-104 11.784976271145803 41.272801653152634 23.271450000074925 23.67077207562664 105-109 29.006714808693136 24.56337259920489 28.03253557829623 18.397377013805745 110-114 39.454813278567904 19.772886318783335 13.68520269551392 27.087097707134838 115-119 15.52363070200545 39.69799095201631 14.416593864214311 30.36178448176392 120-124 9.405047869797746 22.98298745300308 39.4209470262883 28.191017650910865 125-129 20.967286099401946 28.904876290776244 13.554143296205332 36.573694313616485 130-134 18.730105449919154 33.96350536690188 38.40301139914076 8.903377784038206 135-139 34.99495752482651 7.040643998255739 37.62975195217965 20.334646524738098 140-144 17.274845616343313 42.69116825083804 12.31895416217739 27.715031970641256 145-149 33.26493149576447 18.15623730952106 27.507069205536684 21.071761989177784 150-151 33.930688069338906 20.496949789534728 26.122044382772568 19.4503177583538 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.5 25 0.5 26 2.5 27 2.5 28 2.5 29 6.0 30 6.0 31 13.5 32 35.5 33 110.0 34 461.0 35 3994.5 36 99159.0 37 106777.5 38 12150.5 39 1128.5 40 337.0 41 226.0 42 242.0 43 327.5 44 492.0 45 986.0 46 4546.5 47 39224.5 48 46619.5 49 24031.5 50 76798.5 51 73366.0 52 11411.5 53 2992.5 54 3263.5 55 3306.0 56 1380.0 57 1091.5 58 4277.0 59 32538.0 60 33194.0 61 7226.5 62 33243.0 63 34486.0 64 4786.5 65 1007.0 66 677.5 67 439.5 68 332.5 69 294.0 70 201.0 71 58.5 72 40.5 73 30.5 74 5.0 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.340667524811525 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 667331.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 1.9353154717812557 #Duplication Level Percentage of deduplicated Percentage of total 1 72.257037902984 1.3984016339872956 2 8.945837397909896 0.3462603504842878 3 4.356879516773296 0.2529580901249461 4 2.052489195212601 0.15888856380635222 5 1.3528067614840817 0.1309053927915219 6 1.1526133025303713 0.133840221442075 7 0.6273988024082492 0.08499502264944007 8 0.5941341832970798 0.09198696617991672 9 0.49175098832374464 0.085652396637 >10 5.331886697155085 2.3018713335448324 >50 1.3640217557315761 1.872305805973749 >100 1.1301894547490203 4.183725550167735 >500 0.11691615049127795 1.6668529774419856 >1k 0.171477020720541 7.701614987898463 >5k 0.007794410032751863 1.3502263349396573 >10k+ 0.04676646019651118 78.23951437193074 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG 191983 28.76878190882785 No Hit TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC 120243 18.018494570160833 No Hit TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG 70171 10.51517163146924 No Hit TGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG 62213 9.322659969340553 No Hit TGCAGTTGGTCGACACCCCACACATTGCACTTTAAGGAGCAAGCATGACC 58963 8.835645279479 No Hit TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAACCCCCAAC 15097 2.262295622412266 No Hit TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAAGGCGCCAA 8951 1.3413133812156186 No Hit NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG 4798 0.7189835329094557 No Hit TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG 4718 0.7069954790051713 No Hit TTCCAGATCTCGATGCCGACGACGGGAAAGCAATAGAATCCAAAGTACCT 3388 0.5076940828464436 No Hit TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG 3338 0.5002015491562658 No Hit TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC 3187 0.477574097411929 No Hit TTCCAGATCTCGATGCCGACAGGGAAAGCAATAGAATCCAAAGTACCTGT 3097 0.46408753676960907 No Hit NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC 2990 0.4480535146726287 No Hit TGCAGTTGGTCGACACCCCACACATTGCACTTTCAGGAGCAAGCATGACC 2518 0.3773239966373509 No Hit TGCAGTTGGTCGACAGTCCTCGGTACGTGAGAAAACGAGAAAGTGAGCTG 2082 0.311989102859001 No Hit TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG 2078 0.31138970016378675 No Hit TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG 2063 0.30914194005673346 No Hit NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG 1953 0.29265836593834244 No Hit NGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG 1694 0.2538470414232218 No Hit TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG 1685 0.2524983853589898 No Hit TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC 1640 0.2457551050378298 No Hit TGCAGTTGGTCGACAGTCCTCGGTCCGCAACGGTGTGGACATGGCAGAGG 1640 0.2457551050378298 No Hit TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC 1624 0.24335749425697292 No Hit TGCAGGTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG 1529 0.22912168024563523 No Hit TGCAGTTGGTCGACAGTCCTCGGCACGTGAGAAAACGAGAAAGTGAGCTG 1446 0.2166840743199402 No Hit NGCAGTTGGTCGACACCCCACACATTGCACTTTAAGGAGCAAGCATGACC 1314 0.19690378537787095 No Hit TGCAGGTGGTCGACACCCCACACATTGCACTTTAAGGAGCAAGCATGACC 1160 0.17382678161212353 No Hit TTCCAGATCTCGATGCCGCAGCTCGGACTGTTGACCATCCTCGTGCTGCT 1114 0.16693365061716 No Hit TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG 966 0.1447557508942339 No Hit TGCAGATGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG 942 0.1411593347229486 No Hit TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC 920 0.13786261989927037 No Hit TTCCAGATCTCGATGCCGACAGGGAAAAGCAATAGAATCCAAAGTACCTG 894 0.13396650238037797 No Hit TTCCAGATCTCGATGCCGACAGGGGAAAGCAATAGAATCCAAAGTACCTG 836 0.12527516329977176 No Hit TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG 826 0.12377665656173624 No Hit TTCCAGATCTCGATGCCGACAGAGAATACGAGACACGAGTTATAGCAAAG 823 0.12332710454032556 No Hit TCCCGCAGATGATGAAGATGAAGCAGCGTTCGCAATTTTTCCGTCGGCAC 706 0.10579457570530967 No Hit TTCCAGATCTCGATGCCGACGGGAAAGCAATAGAATCCAAAGTACCTGTC 674 0.1009993541435959 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 4.495520214106643E-4 6 0.0 0.0 4.495520214106643E-4 7 0.0 0.0 4.495520214106643E-4 8 0.0 0.0 4.495520214106643E-4 9 0.0 0.0 4.495520214106643E-4 10-11 0.0 0.0 4.495520214106643E-4 12-13 0.0 0.0 4.495520214106643E-4 14-15 0.0 0.0 4.495520214106643E-4 16-17 0.0 0.0 4.495520214106643E-4 18-19 0.0 0.0 4.495520214106643E-4 20-21 0.0 0.0 4.495520214106643E-4 22-23 0.0 0.0 0.002997013476071095 24-25 0.0 0.0 0.005094922909320862 26-27 0.0 0.0 0.005094922909320862 28-29 0.0 0.0 0.005244773583124416 30-31 0.0 0.0 0.0053196989200261936 32-33 0.0 0.0 0.006143877625945746 34-35 0.0 0.0 0.007492533690177738 36-37 0.0 0.0 0.013186859294712819 38-39 0.0 0.0 0.01858148355164079 40-41 0.0 0.0 0.020079990289676337 42-43 0.0 0.0 0.07027996601386718 44-45 0.0 0.0 0.11598442152395137 46-47 0.0 0.0 0.7012262280637345 48-49 0.0 0.0 2.3902680978405018 50-51 0.0 0.0 2.891069049691982 52-53 0.0 0.0 2.953631706004966 54-55 0.0 0.0 2.954306034037082 56-57 0.0 0.0 2.954680660721591 58-59 0.0 0.0 2.958052300882171 60-61 0.0 0.0 2.9659194612568576 62-63 0.0 0.0 2.980604827289606 64-65 0.0 0.0 2.9848755714930073 66-67 0.0 0.0 2.9877976596321765 68-69 0.0 0.0 2.998811684156738 70-71 0.0 0.0 2.999111385504345 72-73 0.0 0.0 3.0022582496542194 74-75 0.0 0.0 3.0066039191945224 76-77 0.0 0.0 3.0221883892700925 78-79 0.0 0.0 3.023312269323619 80-81 0.0 0.0 3.0234621199974225 82-83 0.0 0.0 3.031404205709011 84-85 0.0 0.0 3.0392713660836974 86-87 0.0 0.0 3.0392713660836974 88-89 0.0 0.0 3.0392713660836974 90-91 0.0 0.0 3.0395710674313046 92-93 0.0 0.0 3.0398707687789117 94-95 0.0 0.0 3.040470171474126 96-97 0.0 0.0 3.041519126190751 98-99 0.0 0.0 3.071788962299069 100-101 0.0 0.0 3.0780826905988183 102-103 0.0 0.0 3.8552832102809553 104-105 0.0 0.0 4.580185844805651 106-107 0.0 0.0 4.580635396827061 108-109 0.0 0.0 4.5835574849662315 110-111 0.0 0.0 4.679237140189801 112-113 0.0 0.0 4.771695005926595 114-115 0.0 0.0 4.777089630183522 116-117 0.0 0.0 4.779187539616772 118-119 0.0 0.0 4.784357387862994 120-121 0.0 0.0 4.800990812655189 122-123 0.0 0.0 4.829387515340963 124-125 0.0 0.0 5.428190807859967 126-127 0.0 0.0 5.430288717293218 128-129 0.0 0.0 5.431037970662235 130-131 0.0 0.0 5.431112895999137 132-133 0.0 0.0 5.432012000041958 134-135 0.0 0.0 5.437631400309591 136-137 0.0 0.0 5.559609848785685 138-139 0.0 0.0 5.562232235577247 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGAGTTG 10 0.006609348 148.44699 1 CTCCAGA 20 1.894974E-6 148.44699 1 TGCAGGG 10 0.006609348 148.44699 1 TGCAGTT 14020 0.0 148.3411 1 TTCCAGA 18880 0.0 147.89659 1 TCCCGCA 8505 0.0 147.74883 1 CTAGATG 25 3.7136488E-8 144.97664 9 AGATCCC 25 3.7136488E-8 144.97664 5 ATCAAGT 25 3.7136488E-8 144.97664 145 CCATATC 100 0.0 144.97664 3 GATCTTG 25 3.7136488E-8 144.97664 6 GATCTAG 25 3.7136488E-8 144.97664 6 CTTAGGT 25 3.7136488E-8 144.97664 145 GTTGATC 10 0.0070937555 144.97662 5 TTGATCG 10 0.0070937555 144.97662 6 TTTAGGT 10 0.0070937555 144.97662 145 TCGCAGA 20 2.131792E-6 144.97662 3 GTTCTGA 10 0.0070937555 144.97662 145 CATATGA 10 0.0070937555 144.97662 6 ATCTAGT 20 2.131792E-6 144.97662 145 >>END_MODULE