FastQCFastQC Report
Wed 17 Jun 2015
000000000-AG1W8 l01n01 mis09.3410000000d1ea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG1W8 l01n01 mis09.3410000000d1ea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667331
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG19198328.76878190882785No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC12024318.018494570160833No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG7017110.51517163146924No Hit
TGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG622139.322659969340553No Hit
TGCAGTTGGTCGACACCCCACACATTGCACTTTAAGGAGCAAGCATGACC589638.835645279479No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAACCCCCAAC150972.262295622412266No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAAGGCGCCAA89511.3413133812156186No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG47980.7189835329094557No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG47180.7069954790051713No Hit
TTCCAGATCTCGATGCCGACGACGGGAAAGCAATAGAATCCAAAGTACCT33880.5076940828464436No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG33380.5002015491562658No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC31870.477574097411929No Hit
TTCCAGATCTCGATGCCGACAGGGAAAGCAATAGAATCCAAAGTACCTGT30970.46408753676960907No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC29900.4480535146726287No Hit
TGCAGTTGGTCGACACCCCACACATTGCACTTTCAGGAGCAAGCATGACC25180.3773239966373509No Hit
TGCAGTTGGTCGACAGTCCTCGGTACGTGAGAAAACGAGAAAGTGAGCTG20820.311989102859001No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG20780.31138970016378675No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG20630.30914194005673346No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG19530.29265836593834244No Hit
NGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG16940.2538470414232218No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG16850.2524983853589898No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC16400.2457551050378298No Hit
TGCAGTTGGTCGACAGTCCTCGGTCCGCAACGGTGTGGACATGGCAGAGG16400.2457551050378298No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC16240.24335749425697292No Hit
TGCAGGTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG15290.22912168024563523No Hit
TGCAGTTGGTCGACAGTCCTCGGCACGTGAGAAAACGAGAAAGTGAGCTG14460.2166840743199402No Hit
NGCAGTTGGTCGACACCCCACACATTGCACTTTAAGGAGCAAGCATGACC13140.19690378537787095No Hit
TGCAGGTGGTCGACACCCCACACATTGCACTTTAAGGAGCAAGCATGACC11600.17382678161212353No Hit
TTCCAGATCTCGATGCCGCAGCTCGGACTGTTGACCATCCTCGTGCTGCT11140.16693365061716No Hit
TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG9660.1447557508942339No Hit
TGCAGATGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG9420.1411593347229486No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC9200.13786261989927037No Hit
TTCCAGATCTCGATGCCGACAGGGAAAAGCAATAGAATCCAAAGTACCTG8940.13396650238037797No Hit
TTCCAGATCTCGATGCCGACAGGGGAAAGCAATAGAATCCAAAGTACCTG8360.12527516329977176No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG8260.12377665656173624No Hit
TTCCAGATCTCGATGCCGACAGAGAATACGAGACACGAGTTATAGCAAAG8230.12332710454032556No Hit
TCCCGCAGATGATGAAGATGAAGCAGCGTTCGCAATTTTTCCGTCGGCAC7060.10579457570530967No Hit
TTCCAGATCTCGATGCCGACGGGAAAGCAATAGAATCCAAAGTACCTGTC6740.1009993541435959No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGTTG100.006609348148.446991
CTCCAGA201.894974E-6148.446991
TGCAGGG100.006609348148.446991
TGCAGTT140200.0148.34111
TTCCAGA188800.0147.896591
TCCCGCA85050.0147.748831
CTAGATG253.7136488E-8144.976649
AGATCCC253.7136488E-8144.976645
ATCAAGT253.7136488E-8144.97664145
CCATATC1000.0144.976643
GATCTTG253.7136488E-8144.976646
GATCTAG253.7136488E-8144.976646
CTTAGGT253.7136488E-8144.97664145
GTTGATC100.0070937555144.976625
TTGATCG100.0070937555144.976626
TTTAGGT100.0070937555144.97662145
TCGCAGA202.131792E-6144.976623
GTTCTGA100.0070937555144.97662145
CATATGA100.0070937555144.976626
ATCTAGT202.131792E-6144.97662145
Produced by FastQC (version 0.11.2)