##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AG1W8 l01n01 mis08.3410000000d1dd.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 766966 Sequences flagged as poor quality 0 Sequence length 151 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.05495680382181 34.0 34.0 34.0 34.0 34.0 2 33.54653139774123 34.0 34.0 34.0 34.0 34.0 3 33.857657314665836 34.0 34.0 34.0 34.0 34.0 4 33.76015625203725 34.0 34.0 34.0 34.0 34.0 5 33.8032585538342 34.0 34.0 34.0 34.0 34.0 6 36.430264183809975 38.0 38.0 38.0 34.0 38.0 7 37.12669270867288 38.0 38.0 38.0 36.0 38.0 8 37.231873381610136 38.0 38.0 38.0 36.0 38.0 9 37.445425220935476 38.0 38.0 38.0 37.0 38.0 10-14 37.31904569433326 38.0 38.0 38.0 36.6 38.0 15-19 37.2372973508604 38.0 38.0 38.0 36.2 38.0 20-24 37.46209662488298 38.0 38.0 38.0 37.0 38.0 25-29 37.190621227016585 38.0 38.0 38.0 36.2 38.0 30-34 37.01146804421578 38.0 38.0 38.0 35.8 38.0 35-39 37.05250975923313 38.0 38.0 38.0 35.8 38.0 40-44 37.091099475074515 38.0 38.0 38.0 36.2 38.0 45-49 36.86048221172777 38.0 38.0 38.0 35.2 38.0 50-54 37.02117747070926 38.0 38.0 38.0 36.2 38.0 55-59 36.5707525496567 38.0 37.6 38.0 34.4 38.0 60-64 36.53678468145915 38.0 37.2 38.0 34.0 38.0 65-69 36.62934132673417 38.0 37.4 38.0 34.4 38.0 70-74 36.603232216291204 38.0 37.8 38.0 34.4 38.0 75-79 36.320697658044814 38.0 37.0 38.0 33.4 38.0 80-84 36.36514291376672 38.0 37.2 38.0 33.8 38.0 85-89 36.00667695830063 38.0 37.0 38.0 32.2 38.0 90-94 36.209852066454054 38.0 37.0 38.0 33.6 38.0 95-99 35.94279772506213 38.0 36.8 38.0 32.2 38.0 100-104 35.81912418542673 38.0 36.8 38.0 31.4 38.0 105-109 36.03683240195784 38.0 37.0 38.0 32.4 38.0 110-114 35.86764602342216 38.0 37.0 38.0 31.4 38.0 115-119 35.28486217120446 38.0 36.0 38.0 28.2 38.0 120-124 35.3344745399405 38.0 36.4 38.0 28.6 38.0 125-129 35.41189205258121 38.0 37.0 38.0 29.2 38.0 130-134 35.185421518033394 38.0 36.2 38.0 28.0 38.0 135-139 35.064700130123114 38.0 36.2 38.0 27.4 38.0 140-144 35.24505571303031 38.0 36.6 38.0 28.0 38.0 145-149 34.951873225149484 37.8 35.4 38.0 26.8 38.0 150-151 32.76596811331923 35.5 31.5 37.5 21.5 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.877319299567219 1101 2 0.3921497077686311 1101 3 0.06511689021971279 1101 4 0.08893580492294006 1101 5 0.12271750619286337 1101 6 1.2428929464884817 1101 7 0.4522097798468252 1101 8 0.4129868006509838 1101 9 0.19074538987156586 1101 10-14 0.29800606436446486 1101 15-19 0.30176602669449437 1101 20-24 0.16539586759947866 1101 25-29 0.3013521360926745 1101 30-34 0.29725235059584776 1101 35-39 0.2325462316302307 1101 40-44 0.2758375181576156 1101 45-49 0.3633210684686148 1101 50-54 0.26611036045986225 1101 55-59 0.4218557775783154 1101 60-64 0.4556970453809157 1101 65-69 0.4010457242888208 1101 70-74 0.37829479371733754 1101 75-79 0.5258472801366807 1101 80-84 0.5595643005422772 1101 85-89 0.6047289541462533 1101 90-94 0.45834530061652856 1101 95-99 0.5661779795395532 1101 100-104 0.7724966623785434 1101 105-109 0.5331225781589382 1101 110-114 0.6010873302431747 1101 115-119 0.7927983527993234 1101 120-124 0.6052302735754438 1101 125-129 0.513582717956524 1101 130-134 0.6255586929309729 1101 135-139 0.7303921374598659 1101 140-144 0.5665582528381208 1101 145-149 0.7934038531284742 1101 150-151 0.6802662843650822 1102 1 0.8867814853815119 1102 2 0.37255158149299916 1102 3 0.07490796045522075 1102 4 0.09059151112642638 1102 5 0.0989691829392001 1102 6 1.0670023138408666 1102 7 0.433085115489952 1102 8 0.16863131773158102 1102 9 0.15102207593904637 1102 10-14 0.23605782734012593 1102 15-19 0.20800829532466025 1102 20-24 0.11749891773801124 1102 25-29 0.2554268471610044 1102 30-34 0.19516328803634764 1102 35-39 0.35611222267723974 1102 40-44 0.32063388016462824 1102 45-49 0.3819304257123335 1102 50-54 0.24820278003834062 1102 55-59 0.34751901215541636 1102 60-64 0.5520567015356619 1102 65-69 0.4653106335200192 1102 70-74 0.33605239908141726 1102 75-79 0.3973334004618323 1102 80-84 0.5439378944379598 1102 85-89 0.48866599452498605 1102 90-94 0.5149772702510163 1102 95-99 0.5770070926988424 1102 100-104 0.8953952931003428 1102 105-109 0.5098641257823004 1102 110-114 0.35559464539437613 1102 115-119 0.7645923879314296 1102 120-124 0.7245023834712754 1102 125-129 0.4867710913867995 1102 130-134 0.500863163737371 1102 135-139 0.6568028494534914 1102 140-144 0.5115505820027622 1102 145-149 0.7050360178838133 1102 150-151 0.8864323853380114 1103 1 0.8839328653497986 1103 2 0.31635756052378383 1103 3 0.0422313709674782 1103 4 0.08914176700314158 1103 5 0.1088796068921809 1103 6 1.242324415216416 1103 7 0.5079818434160259 1103 8 0.25603562790100654 1103 9 0.19592671243285764 1103 10-14 0.1640805030466126 1103 15-19 0.2522907079752912 1103 20-24 0.12485882859386521 1103 25-29 0.2404591148852191 1103 30-34 0.2399741062659757 1103 35-39 0.17984641993842132 1103 40-44 0.26044142555251426 1103 45-49 0.276331322912462 1103 50-54 0.16560978718292318 1103 55-59 0.3158749994402896 1103 60-64 0.4299570055932236 1103 65-69 0.3800192738222208 1103 70-74 0.24443557686679185 1103 75-79 0.3471361661570711 1103 80-84 0.4117405699141017 1103 85-89 0.477980934039806 1103 90-94 0.42114038638027296 1103 95-99 0.4804334028912862 1103 100-104 0.605069707731964 1103 105-109 0.32894743256178316 1103 110-114 0.45310224683326794 1103 115-119 0.9297865797410694 1103 120-124 0.7810634234095417 1103 125-129 0.6500298435990857 1103 130-134 0.6466960971633497 1103 135-139 0.5678380368212785 1103 140-144 0.6902258585147081 1103 145-149 0.8549670578604776 1103 150-151 0.946946365459091 2116 1 0.7147640641607893 2116 2 0.24213547533262414 2116 3 -0.1019714244662282 2116 4 -0.15546658767541288 2116 5 -0.005339078874506242 2116 6 1.050304366517686 2116 7 -0.12131091818154971 2116 8 0.11070825541894891 2116 9 0.06564179322848673 2116 10-14 0.11322392713667284 2116 15-19 0.14135562907702592 2116 20-24 0.07835193487726144 2116 25-29 0.20122975650682662 2116 30-34 0.23310133960879398 2116 35-39 0.12795341484719103 2116 40-44 -0.048404193142744134 2116 45-49 0.16232925163523504 2116 50-54 0.1103802074466671 2116 55-59 0.22671639652468656 2116 60-64 0.3295961132047651 2116 65-69 0.2672496915561595 2116 70-74 0.17479797132651953 2116 75-79 0.18238819790612126 2116 80-84 0.24972755723345585 2116 85-89 0.36721147826394684 2116 90-94 0.2912001530058248 2116 95-99 0.3868140435642218 2116 100-104 0.5497084991179833 2116 105-109 0.22734614552387455 2116 110-114 0.27118837085692604 2116 115-119 0.5651631252933527 2116 120-124 0.4275722935357109 2116 125-129 0.27715804142978584 2116 130-134 0.41764129527864924 2116 135-139 0.6062740643961533 2116 140-144 0.45689568743668474 2116 145-149 0.5607148521873953 2116 150-151 1.3127402496573026 1117 1 0.8583783530829336 1117 2 0.3614422422319876 1117 3 0.09558649648030126 1117 4 0.09323802791708857 1117 5 0.09355600666309272 1117 6 1.2286116083499294 1117 7 0.36237245459705036 1117 8 0.29382121194569777 1117 9 0.20928887040408028 1117 10-14 0.2500754400661762 1117 15-19 0.20733264947926955 1117 20-24 0.12764137368147033 1117 25-29 0.288301844959598 1117 30-34 0.24043184917800176 1117 35-39 0.1264040705128977 1117 40-44 0.23807100005956272 1117 45-49 0.32606643689283743 1117 50-54 0.22473162598573282 1117 55-59 0.39509267253422564 1117 60-64 0.5053836771170168 1117 65-69 0.3660434315830656 1117 70-74 0.2737861556322727 1117 75-79 0.36164436160097324 1117 80-84 0.38061205514593865 1117 85-89 0.4854898366256464 1117 90-94 0.3027973582543311 1117 95-99 -0.08339375447603459 1117 100-104 0.4457417165994926 1117 105-109 0.4688869748480968 1117 110-114 0.44913519029698534 1117 115-119 0.824115759183691 1117 120-124 0.6569058573804014 1117 125-129 0.5287214946488916 1117 130-134 0.4761066758509074 1117 135-139 0.38285053355829746 1117 140-144 0.5215676359929162 1117 145-149 -0.5689920506341863 1117 150-151 0.015170952180405095 2117 1 -30.9664608354376 2117 2 -10.581253120372512 2117 3 -1.8013316753872672 2117 4 -0.3819103793711207 2117 5 0.034986686998905725 2117 6 1.1876363390358549 2117 7 0.025686990809788313 2117 8 0.22729194555137866 2117 9 0.21696692848023247 2117 10-14 0.3966415646287942 2117 15-19 0.39076948283918256 2117 20-24 0.2979269290689004 2117 25-29 0.3530343301733865 2117 30-34 0.4009431199533182 2117 35-39 0.24229989166354216 2117 40-44 0.13193119800864395 2117 45-49 0.4261275854664319 2117 50-54 0.2948307368369498 2117 55-59 0.47195138606964093 2117 60-64 0.5813867468135783 2117 65-69 0.44880948444672697 2117 70-74 0.38284287822684604 2117 75-79 0.4924940759638048 2117 80-84 0.5651676647710531 2117 85-89 0.5854430823663037 2117 90-94 0.4497122177939019 2117 95-99 -0.17409936212465738 2117 100-104 0.6907493567634404 2117 105-109 0.6936088824218771 2117 110-114 0.7540799371219862 2117 115-119 1.0169051346531148 2117 120-124 0.8471806442941201 2117 125-129 0.5579574373606135 2117 130-134 0.7518208233081225 2117 135-139 0.6797155991301267 2117 140-144 0.7447791220013968 2117 145-149 0.9751456331625334 2117 150-151 1.2775183704047492 1116 1 0.2496042382787209 1116 2 0.19478567960675974 1116 3 -0.07973581196028334 1116 4 -0.28054417305506263 1116 5 0.07343177474842122 1116 6 1.2026986411250107 1116 7 -0.19932403827758804 1116 8 0.24622388172692666 1116 9 0.13918686318803708 1116 10-14 0.2403907540784047 1116 15-19 0.22318707279801941 1116 20-24 0.09402872439425636 1116 25-29 0.04977002842316125 1116 30-34 0.12462222743054241 1116 35-39 0.17462137800637834 1116 40-44 0.021901380958446737 1116 45-49 0.20614845320000796 1116 50-54 0.09642923323971075 1116 55-59 0.2298015893549774 1116 60-64 0.3538292940215797 1116 65-69 0.2755530115978928 1116 70-74 0.23063693470417945 1116 75-79 0.21447771510047176 1116 80-84 0.30231702032221364 1116 85-89 0.3184304874986523 1116 90-94 0.4089052387274563 1116 95-99 -0.5670479728797986 1116 100-104 0.564282095996596 1116 105-109 0.3877891376244591 1116 110-114 0.4528835297334126 1116 115-119 0.9067128013743968 1116 120-124 0.6127610403889179 1116 125-129 0.24389815899205303 1116 130-134 0.35964709172319687 1116 135-139 0.6192830908425648 1116 140-144 0.292315454966392 1116 145-149 0.3899216131407073 1116 150-151 0.62161581291695 2118 1 0.803147007699657 2118 2 0.3086958344160138 2118 3 0.04215803454628997 2118 4 -0.01955824843610543 2118 5 0.036185844347791374 2118 6 1.173060095315229 2118 7 0.39921235136723254 2118 8 0.07373093437004741 2118 9 0.07284117900689324 2118 10-14 0.16438504218510275 2118 15-19 0.15421057828562112 2118 20-24 0.03158841019027392 2118 25-29 0.16898312291363027 2118 30-34 0.19331631716608655 2118 35-39 0.04696121073662596 2118 40-44 0.030659118929072804 2118 45-49 0.21579133526386585 2118 50-54 0.017219328637310127 2118 55-59 0.20430675797047826 2118 60-64 0.31322356418515795 2118 65-69 0.27192486282948636 2118 70-74 0.23434911708602613 2118 75-79 0.2578594948586357 2118 80-84 0.27665526807683705 2118 85-89 0.10626061599131731 2118 90-94 0.25598753783928174 2118 95-99 0.3385598503206566 2118 100-104 0.5414696614142258 2118 105-109 0.17075035057938948 2118 110-114 0.4831975841808145 2118 115-119 0.7368581406488985 2118 120-124 0.3210001224986243 2118 125-129 0.1964965720227454 2118 130-134 0.30293895651181657 2118 135-139 0.3729395498674464 2118 140-144 0.17918602525856642 2118 145-149 0.14921709645859238 2118 150-151 1.1247736774862602 1119 1 0.7634843563909968 1119 2 0.2813393761376375 1119 3 -0.01489741842712533 1119 4 -0.04484547842525899 1119 5 0.1107718521440404 1119 6 1.2794744099199917 1119 7 0.2849028757085108 1119 8 0.355782723695647 1119 9 0.05189097530806919 1119 10-14 0.15312313532969313 1119 15-19 0.1716408957040727 1119 20-24 0.04204812471883912 1119 25-29 0.23052361354934447 1119 30-34 0.1434087876370782 1119 35-39 0.10485549251511372 1119 40-44 0.040517262485458616 1119 45-49 0.12866935044601746 1119 50-54 0.13289387947408215 1119 55-59 0.30635708183693566 1119 60-64 0.33069227663276735 1119 65-69 0.110040135844514 1119 70-74 0.13012375878127358 1119 75-79 0.29194871201036676 1119 80-84 0.1903622934878939 1119 85-89 0.3695688899735643 1119 90-94 0.3205795540179466 1119 95-99 0.28576170266099155 1119 100-104 0.5388267058762608 1119 105-109 0.3067906800146005 1119 110-114 0.4372289156969771 1119 115-119 0.8388766640522789 1119 120-124 0.5630781435426186 1119 125-129 0.2981113614362698 1119 130-134 0.5395968304237115 1119 135-139 0.5000890853169082 1119 140-144 0.4864908993756245 1119 145-149 0.5692699995331267 1119 150-151 1.058014320529736 2119 1 0.8999121454728254 2119 2 0.33147447597542623 2119 3 0.06531961929766794 2119 4 0.04299465818910164 2119 5 0.053064137515278276 2119 6 1.1805102119257498 2119 7 0.38672710763685103 2119 8 0.2538992278254639 2119 9 0.02509708871291849 2119 10-14 0.10955491924018901 2119 15-19 0.04282435272344998 2119 20-24 0.037818435239948656 2119 25-29 0.1311638836173188 2119 30-34 0.043241312127356935 2119 35-39 0.09360412340830493 2119 40-44 -0.1909483786955235 2119 45-49 0.15566824638073484 2119 50-54 0.12347310768196706 2119 55-59 0.28303167231212 2119 60-64 0.2773200485939 2119 65-69 0.24572910111805157 2119 70-74 0.1660618521066155 2119 75-79 0.1621527775167806 2119 80-84 0.20513789166958674 2119 85-89 0.4331569046095609 2119 90-94 0.30228148273000954 2119 95-99 0.2664807279227901 2119 100-104 0.6216149206926858 2119 105-109 0.3588946596980378 2119 110-114 0.37518615347049433 2119 115-119 0.9041188011539205 2119 120-124 0.4800954551985157 2119 125-129 0.5070852392076759 2119 130-134 0.4322865134494194 2119 135-139 0.25724564528652394 2119 140-144 0.22211928373081946 2119 145-149 0.2667249424751219 2119 150-151 0.31851665926046735 1118 1 0.8804337240231561 1118 2 0.3908432094950811 1118 3 0.08064420898718083 1118 4 0.07471823442473635 1118 5 0.12330621970063049 1118 6 1.2260470580348368 1118 7 0.49136897810073776 1118 8 0.40098201294302527 1118 9 0.19657356074148424 1118 10-14 0.16761665452190044 1118 15-19 0.18871608679620522 1118 20-24 0.14321316119805516 1118 25-29 0.23333970810909221 1118 30-34 0.15285415926488355 1118 35-39 0.04512914714566563 1118 40-44 0.08841351582504586 1118 45-49 0.18379576284134913 1118 50-54 0.1895238402349193 1118 55-59 0.24120211987064266 1118 60-64 0.26447013021648047 1118 65-69 0.05251163485172583 1118 70-74 0.1819814959884738 1118 75-79 -0.07132787002473151 1118 80-84 -0.2562078357588291 1118 85-89 0.17976685141761095 1118 90-94 0.16627570799419544 1118 95-99 0.1375113924204001 1118 100-104 0.3221565967564928 1118 105-109 0.3447218592598915 1118 110-114 0.22755770955016885 1118 115-119 0.7505959978524359 1118 120-124 0.6004055620938118 1118 125-129 0.29171530756703135 1118 130-134 0.4401418415175229 1118 135-139 0.5055317595967921 1118 140-144 0.37231199880638854 1118 145-149 0.6457562696627832 1118 150-151 1.017844731136421 2112 1 0.8982224659059668 2112 2 0.3359859251164039 2112 3 0.06695668721273051 2112 4 0.08057065149238696 2112 5 0.07940777803489851 2112 6 1.256026487246281 2112 7 0.45098401972747837 2112 8 0.4185307160869556 2112 9 0.10134217633480347 2112 10-14 0.18799518105831936 2112 15-19 0.21983268509380594 2112 20-24 0.12391557138105469 2112 25-29 0.24507924274352177 2112 30-34 0.28609585124459613 2112 35-39 0.16580713298267824 2112 40-44 0.17756552064523845 2112 45-49 0.19103370476445747 2112 50-54 0.20646512292431396 2112 55-59 0.32892927876381606 2112 60-64 0.3864419225457709 2112 65-69 0.18245118852291142 2112 70-74 0.1686935151139508 2112 75-79 0.3994352729625774 2112 80-84 0.14315558797446215 2112 85-89 0.2835416742879602 2112 90-94 0.3336995170647441 2112 95-99 0.29384832271536965 2112 100-104 0.4725665339444376 2112 105-109 0.33806772666609675 2112 110-114 0.3885457080752843 2112 115-119 0.8034280853254216 2112 120-124 0.494096355233566 2112 125-129 0.4008210234923908 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15280.0 32 22300.0 33 28104.0 34 35533.0 35 56199.0 36 113379.0 37 450849.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.10376394703631739 0.07892470102762364 99.63555751420577 0.1817538377302805 2 51.71467835601579 0.03768094022420811 31.34428384048315 16.903356863276862 3 0.061410805694124646 0.09961328142316608 0.10078673630904107 99.73818917657367 4 0.3277850647877481 25.90258238305218 0.16102408711729072 73.60860846504278 5 68.25830610483385 31.382486316212194 0.1788866781578323 0.18032090079612395 6 32.14757890180269 26.41942406834201 25.023143138026978 16.409853891828323 7 0.6318402641055797 47.613975065387514 25.9200538224641 25.834130848042808 8 68.21619211281856 0.28149878873379003 31.098901385459072 0.403407712988581 9 51.46642745571511 16.756675002542483 0.6996398797339126 31.077257662008485 10-14 18.248423007017262 40.76316290422261 24.317818521290384 16.670595567469746 15-19 31.40074005888136 21.521084376621648 18.9093388755173 28.16883668897969 20-24 34.769781189778946 23.500728845868004 10.237429038575375 31.492060925777675 25-29 24.78328895935413 45.36268882844872 5.6941246417702995 24.15989757042685 30-34 21.85859607857454 17.511050033508656 38.41002078318987 22.220333104726937 35-39 42.68770714738332 19.38393618491563 18.710842462377734 19.21751420532331 40-44 13.391910462784532 33.14376386958483 27.151659917128008 26.312665750502628 45-49 16.890083784678854 25.559177329894677 22.801427964212234 34.74931092121424 50-54 28.48097568862244 25.179030100421663 18.12607077758336 28.213923433372535 55-59 22.33400176800536 41.14508335441206 29.982945788991948 6.5379690885906285 60-64 34.89500186448943 20.925464753326743 19.40837012331707 24.771163258866753 65-69 19.349045459642277 33.631895025333584 26.696124730431336 20.322934784592796 70-74 24.735933535515265 13.44166494994563 29.861454093141027 31.960947421398078 75-79 28.807378684322384 17.452638056967324 28.958728287825014 24.78125497088528 80-84 24.78967776928834 20.820036351024687 31.991066096802207 22.399219782884767 85-89 30.60161728160049 22.205625803490637 36.00183058962196 11.19092632528691 90-94 27.529903542008384 34.90245982220855 26.994521269521726 10.573115366261348 95-99 16.064597387628655 31.463950162067157 30.24812051642446 22.223331933879727 100-104 10.33782462325579 37.30217506382291 29.9865443839753 22.373455928946004 105-109 18.912937470500648 25.00170802877833 32.95937499185101 23.125979508870014 110-114 41.836117898316225 26.344166494994564 11.492634614832992 20.32708099185622 115-119 8.674882589319473 40.623208851500586 17.70858160596428 32.99332695321566 120-124 5.7088319430066 21.157965281381443 43.66321323239883 29.469989543213128 125-129 21.010005658660226 29.541179139622876 18.86818972418595 30.580625477530948 130-134 18.75613782097251 29.89316345183489 44.20657499810943 7.144123729083167 135-139 30.920588396356553 1.9359919474918053 45.56752189797201 21.575897758179632 140-144 11.603200141857657 39.68835645908684 17.0438846050542 31.664558794001294 145-149 24.223785669768933 19.665747894952318 33.882362451529794 22.228103983748955 150-151 40.27264572353925 16.82512653755186 28.467102844193874 14.43512489471502 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 0.5 24 0.0 25 0.0 26 0.0 27 0.0 28 2.0 29 2.5 30 2.0 31 9.0 32 27.5 33 83.0 34 398.5 35 3642.0 36 87794.5 37 94415.0 38 10767.5 39 1058.0 40 351.0 41 242.0 42 235.5 43 322.0 44 533.5 45 1145.0 46 6310.5 47 56713.0 48 59003.0 49 16502.0 50 108701.0 51 111610.5 52 15263.0 53 5051.5 54 1842.5 55 602.5 56 286.0 57 238.0 58 285.0 59 540.5 60 1341.5 61 7123.5 62 80047.5 63 83023.0 64 10060.5 65 1084.0 66 190.5 67 55.5 68 23.0 69 8.0 70 9.5 71 10.0 72 6.0 73 1.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.3665977370574445 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 766966.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 1.346815894339673 #Duplication Level Percentage of deduplicated Percentage of total 1 71.83818311720859 0.9675280684274048 2 9.485962792863846 0.2555169092508757 3 3.8454575175026333 0.15537369916741586 4 2.3459437950959643 0.12638217561851114 5 1.3996971106451725 0.09425671579391172 6 1.125654551968682 0.09096296652763344 7 0.9505109519287539 0.0896114280461105 8 0.48488253989905855 0.05224380092990743 9 0.45576937346132823 0.055245369269785606 >10 4.567307670226808 1.354988315524889 >50 1.5903141689436577 1.5548078084602936 >100 1.5612230560971272 3.915686494183665 >500 0.11636445138612128 1.0589148854259607 >1k 0.17454667707918192 6.78512642359829 >5k 0.019394075231020214 1.400698340675065 >10k+ 0.03878815046204043 82.04265659910028 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC 202729 26.432592839839053 No Hit TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG 170600 22.24348928114154 No Hit TGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG 153747 20.04612981540251 No Hit TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG 101116 13.183896026681758 No Hit TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC 5468 0.7129390351071625 No Hit NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC 5257 0.6854280372272042 No Hit TGCAGTTGGTCGACAGTCCTCGGCACGTGAGAAAACGAGAAAGTGAGCTG 4779 0.6231045443996214 No Hit NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG 4291 0.5594772128099551 No Hit NGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG 4222 0.5504807253515801 No Hit TGCAGTTGGTCGACAGTCCTCGGTCCGCAACGGTGTGGACATGGCAGAGG 4179 0.5448742186746218 No Hit TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG 4129 0.5383550248642053 No Hit TGCAGTTGGTCGACAGTCCTCGGTACGTGAGAAAACGAGAAAGTGAGCTG 3756 0.4897218390384972 No Hit TGCAGGTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG 3730 0.48633185825708053 No Hit TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG 2896 0.37759170549933113 No Hit TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG 2850 0.37159404719374783 No Hit TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG 2815 0.3670306115264562 No Hit NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG 2762 0.3601202660874146 No Hit TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG 2557 0.3333915714647064 No Hit TGCAGATGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG 2240 0.29205988270666494 No Hit TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC 1574 0.20522422115191546 No Hit TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC 1560 0.2033988468849988 No Hit TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC 1292 0.1684559680611657 No Hit TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG 1223 0.15945948060279075 No Hit TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA 1098 0.14316149607674916 No Hit TGCAGCTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG 943 0.12295199526445762 No Hit TTCCATATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC 905 0.11799740796854098 No Hit TTCCAGATCTCGATTCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC 811 0.10574132360495771 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 7.823032572499954E-4 6 0.0 0.0 7.823032572499954E-4 7 0.0 0.0 7.823032572499954E-4 8 0.0 0.0 7.823032572499954E-4 9 0.0 0.0 7.823032572499954E-4 10-11 0.0 0.0 7.823032572499954E-4 12-13 0.0 0.0 7.823032572499954E-4 14-15 0.0 0.0 7.823032572499954E-4 16-17 0.0 0.0 7.823032572499954E-4 18-19 0.0 0.0 7.823032572499954E-4 20-21 0.0 0.0 7.823032572499954E-4 22-23 0.0 0.0 0.001369030700187492 24-25 0.0 0.0 0.0016297984526041573 26-27 0.0 0.0 0.0016949903907083235 28-29 0.0 0.0 0.0016949903907083235 30-31 0.0 0.0 0.0016949903907083235 32-33 0.0 0.0 0.002216525895541654 34-35 0.0 0.0 0.003585556595729146 36-37 0.0 0.0 0.003976708224354144 38-39 0.0 0.0 0.0041070921005624764 40-41 0.0 0.0 0.005215355048333303 42-43 0.0 0.0 0.007301497067666625 44-45 0.0 0.0 0.02372986546991653 46-47 0.0 0.0 0.05925947173668716 48-49 0.0 0.0 1.5081502961017828 50-51 0.0 0.0 1.5588044320087202 52-53 0.0 0.0 1.56193364503772 54-55 0.0 0.0 1.5622596047282409 56-57 0.0 0.0 1.5636938273665324 58-59 0.0 0.0 1.6457704774396777 60-61 0.0 0.0 1.6779752948631361 62-63 0.0 0.0 1.6876237017025528 64-65 0.0 0.0 1.6992930586231982 66-67 0.0 0.0 1.71246183012024 68-69 0.0 0.0 1.7133745172536983 70-71 0.0 0.0 1.7134397091918023 72-73 0.0 0.0 1.713635285006115 74-75 0.0 0.0 1.7142220124490524 76-77 0.0 0.0 1.7217842772691359 78-79 0.0 0.0 1.7339751696946148 80-81 0.0 0.0 1.7433628087816149 82-83 0.0 0.0 1.7440147281626566 84-85 0.0 0.0 1.7442754959150732 86-87 0.0 0.0 1.7470135573154482 88-89 0.0 0.0 1.7470787492535522 90-91 0.0 0.0 1.7471439411916565 92-93 0.0 0.0 1.7476002847583856 94-95 0.0 0.0 1.7525548720543023 96-97 0.0 0.0 1.7572486915978023 98-99 0.0 0.0 1.7580961867931564 100-101 0.0 0.0 1.7582265706693647 102-103 0.0 0.0 1.7619425111413023 104-105 0.0 0.0 1.7680053613849895 106-107 0.0 0.0 1.770873806661573 108-109 0.0 0.0 1.770873806661573 110-111 0.0 0.0 1.7711345744139897 112-113 0.0 0.0 1.771264958290198 114-115 0.0 0.0 1.7715257260426147 116-117 0.0 0.0 1.772047261547448 118-119 0.0 0.0 1.7726991809284898 120-121 0.0 0.0 1.7745245551954063 122-123 0.0 0.0 1.8353486334465936 124-125 0.0 0.0 3.0294041717625033 126-127 0.0 0.0 3.0330549202963364 128-129 0.0 0.0 3.033250496110649 130-131 0.0 0.0 3.033250496110649 132-133 0.0 0.0 3.0361841333253365 134-135 0.0 0.0 3.0363145172015447 136-137 0.0 0.0 3.0365100930158575 138-139 0.0 0.0 3.036575284953962 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCGTTG 15 1.11869376E-4 148.49039 1 TTCCGCA 30 5.511538E-10 148.49039 1 TGCCGCG 10 0.00660377 148.49039 1 TCTCGCA 10 0.00660377 148.49039 1 TCCCGTA 50 0.0 148.49039 1 TCCCGCG 25 3.2192474E-8 148.49039 1 TGCAGTT 18400 0.0 148.49039 1 TGCAGTA 10 0.00660377 148.49039 1 TGCAGGT 425 0.0 148.49039 1 GGCAGTT 10 0.00660377 148.49039 1 TGCGGTT 25 3.2192474E-8 148.49039 1 TCCCGCA 11700 0.0 148.42693 1 TTCCAGA 22555 0.0 148.35872 1 ACGGAGA 45 0.0 144.97635 6 TGTTGGT 10 0.0070940033 144.97633 4 CTTCAGT 35 1.0913936E-11 144.97633 145 GGATGAT 20 2.1320193E-6 144.97633 7 GGTGACA 10 0.0070940033 144.97633 8 CATTGGT 10 0.0070940033 144.97633 3 GGTTGAC 10 0.0070940033 144.97633 8 >>END_MODULE