##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AG1W8 l01n01 mis07.3410000000d1b3.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	753710
Sequences flagged as poor quality	0
Sequence length	151
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.03490599832827	34.0	34.0	34.0	34.0	34.0
2	33.53216489100583	34.0	34.0	34.0	34.0	34.0
3	33.85090154038025	34.0	34.0	34.0	34.0	34.0
4	33.75224555863661	34.0	34.0	34.0	34.0	34.0
5	33.79375091215454	34.0	34.0	34.0	34.0	34.0
6	36.39743004603893	38.0	38.0	38.0	34.0	38.0
7	37.066281461039395	38.0	38.0	38.0	36.0	38.0
8	37.20603945814703	38.0	38.0	38.0	36.0	38.0
9	37.40629817834446	38.0	38.0	38.0	37.0	38.0
10-14	37.286479415159675	38.0	38.0	38.0	36.4	38.0
15-19	37.20504358440249	38.0	38.0	38.0	36.2	38.0
20-24	37.439029069536026	38.0	38.0	38.0	36.6	38.0
25-29	37.156272571678755	38.0	38.0	38.0	36.0	38.0
30-34	37.0332334717597	38.0	38.0	38.0	36.0	38.0
35-39	36.99425879980363	38.0	38.0	38.0	35.8	38.0
40-44	37.02329596263816	38.0	38.0	38.0	36.2	38.0
45-49	36.748187764524815	38.0	37.6	38.0	34.8	38.0
50-54	36.95996152366295	38.0	38.0	38.0	35.8	38.0
55-59	36.472578843321706	38.0	37.4	38.0	33.8	38.0
60-64	36.517872125883954	38.0	37.2	38.0	34.0	38.0
65-69	36.57732682331401	38.0	37.2	38.0	34.2	38.0
70-74	36.486766262886256	38.0	37.2	38.0	34.0	38.0
75-79	36.2092831460376	38.0	37.0	38.0	33.2	38.0
80-84	36.24397739183506	38.0	37.0	38.0	33.6	38.0
85-89	35.8352716562073	38.0	37.0	38.0	31.6	38.0
90-94	36.10524419206327	38.0	37.0	38.0	33.2	38.0
95-99	35.77073622480795	38.0	36.8	38.0	30.8	38.0
100-104	35.67849358506587	38.0	36.8	38.0	30.8	38.0
105-109	35.990018176752336	38.0	37.0	38.0	32.2	38.0
110-114	35.754356715447585	38.0	36.8	38.0	31.0	38.0
115-119	35.20005545899617	38.0	35.6	38.0	28.0	38.0
120-124	35.21720900611641	38.0	36.2	38.0	28.2	38.0
125-129	35.45091799233127	38.0	36.6	38.0	29.6	38.0
130-134	35.10133021984583	38.0	36.2	38.0	27.6	38.0
135-139	34.87340834007775	38.0	35.8	38.0	26.4	38.0
140-144	35.11891576335726	38.0	36.0	38.0	27.4	38.0
145-149	34.839295219646814	37.8	35.4	38.0	26.6	38.0
150-151	32.722166350453094	36.0	31.5	37.5	21.5	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7999602977200624
1101	2	0.3605373320655261
1101	3	0.016392431476596414
1101	4	-4.728573243468759E-4
1101	5	0.08680478133845071
1101	6	1.1602614720924578
1101	7	0.3741501440700148
1101	8	0.2852599148875967
1101	9	0.1528628325515271
1101	10-14	0.18339897876673206
1101	15-19	0.22285698826352984
1101	20-24	0.09055927204092029
1101	25-29	0.208270672970869
1101	30-34	0.1330489876863581
1101	35-39	0.13205758476710372
1101	40-44	0.2176294373023424
1101	45-49	0.259751972454751
1101	50-54	0.1494236302139882
1101	55-59	0.3240999128414259
1101	60-64	0.3520684885628711
1101	65-69	0.2379470982076839
1101	70-74	0.2688359556328024
1101	75-79	0.32437048851434014
1101	80-84	0.4648067190131968
1101	85-89	0.5788016914789509
1101	90-94	0.39897206721067136
1101	95-99	0.47144446623199343
1101	100-104	0.6825095346033407
1101	105-109	0.36658454139165286
1101	110-114	0.4263170870129329
1101	115-119	0.5698956598755629
1101	120-124	0.4480751844562718
1101	125-129	0.4075275806609042
1101	130-134	0.49486802202391544
1101	135-139	0.6371994153010974
1101	140-144	0.534786469327031
1101	145-149	0.6485838761544898
1101	150-151	0.553345696970851
1102	1	0.9115845902002775
1102	2	0.3387825645253102
1102	3	0.06574361006418172
1102	4	0.08741217678112889
1102	5	0.12182478972562905
1102	6	1.1127613950915602
1102	7	0.5429846821712445
1102	8	0.1473986918290251
1102	9	0.22845496945675592
1102	10-14	0.21358119843016254
1102	15-19	0.273539737456197
1102	20-24	0.12441891243487646
1102	25-29	0.2585433983051004
1102	30-34	0.13768401527637764
1102	35-39	0.221612851273143
1102	40-44	0.25143188379856696
1102	45-49	0.3041608780232181
1102	50-54	0.18821455026809986
1102	55-59	0.39200176868152425
1102	60-64	0.4705633934726876
1102	65-69	0.4183946037339723
1102	70-74	0.29141460908848416
1102	75-79	0.36284857168952556
1102	80-84	0.5330767102232485
1102	85-89	0.6069534093097531
1102	90-94	0.5251150330009509
1102	95-99	0.44860419926396133
1102	100-104	0.8002586443544999
1102	105-109	0.3164078657928897
1102	110-114	0.3350015562872031
1102	115-119	0.7766914037397754
1102	120-124	0.7857416544744922
1102	125-129	0.6554978606835533
1102	130-134	0.8113392485653037
1102	135-139	0.7152138346077095
1102	140-144	0.550083728331991
1102	145-149	0.7326108098839583
1102	150-151	1.0950689296817728
1103	1	0.8993919544407234
1103	2	0.38592096377907836
1103	3	0.07314150791876273
1103	4	0.09925717961797176
1103	5	0.1453938691474903
1103	6	1.246827257827043
1103	7	0.4353394023820414
1103	8	0.3354659393263546
1103	9	0.15510778850492102
1103	10-14	0.1612776374766014
1103	15-19	0.2664474969307591
1103	20-24	0.15522394950610163
1103	25-29	0.28569607791544627
1103	30-34	0.24045450061617402
1103	35-39	0.32994334776795853
1103	40-44	0.3370838277541708
1103	45-49	0.2894308585019232
1103	50-54	0.2965748095263052
1103	55-59	0.5440359742224956
1103	60-64	0.5368397303929342
1103	65-69	0.4501636369971109
1103	70-74	0.4057356430439967
1103	75-79	0.4653082549252545
1103	80-84	0.5115188531422206
1103	85-89	0.6133058119677344
1103	90-94	0.5401954261194533
1103	95-99	0.548396725396529
1103	100-104	0.7787542063851021
1103	105-109	0.48822421175255215
1103	110-114	0.5491701702752181
1103	115-119	1.0268990699352472
1103	120-124	0.9385852725494814
1103	125-129	0.7130959239402372
1103	130-134	0.8489373599039993
1103	135-139	1.0150226605412271
1103	140-144	0.8807341817453249
1103	145-149	1.110568815057583
1103	150-151	1.1034913349361801
2116	1	0.6591103514101562
2116	2	0.283275741076892
2116	3	-0.1333284038507756
2116	4	-0.23867853680758344
2116	5	0.034683725060283166
2116	6	1.1965743079531563
2116	7	0.0072349077502806836
2116	8	0.19147368002736442
2116	9	0.13248773999266206
2116	10-14	0.19810196898346533
2116	15-19	0.3187253987272598
2116	20-24	0.17980444409921148
2116	25-29	0.33103493894039104
2116	30-34	0.34403300446719953
2116	35-39	0.18132594713594585
2116	40-44	0.11328518261844778
2116	45-49	0.3758673335354956
2116	50-54	0.2349620668442043
2116	55-59	0.446857464573192
2116	60-64	0.42530106071116336
2116	65-69	0.4246596216896563
2116	70-74	0.3180537945529949
2116	75-79	0.3856294795215476
2116	80-84	0.5147328385885288
2116	85-89	0.6534842056514663
2116	90-94	0.5139568173196025
2116	95-99	0.5791278399221866
2116	100-104	0.8011904011685829
2116	105-109	0.4416649098652172
2116	110-114	0.48086664975415516
2116	115-119	0.8922969284379363
2116	120-124	0.6554263224243329
2116	125-129	0.6694946618484181
2116	130-134	0.6531325106109094
2116	135-139	0.9346815285241021
2116	140-144	0.6628145072219951
2116	145-149	0.9241616696464376
2116	150-151	1.753118674782911
1117	1	0.9277714210220154
1117	2	0.3714782495335598
1117	3	0.10352153721895263
1117	4	0.06814027961699765
1117	5	0.08795215421312008
1117	6	1.2325543294572796
1117	7	0.4458717053576535
1117	8	0.3270971935103617
1117	9	0.23306084998681342
1117	10-14	0.249992261376633
1117	15-19	0.27424585922471323
1117	20-24	0.1577880558017668
1117	25-29	0.29612558767086483
1117	30-34	0.27190854685614596
1117	35-39	0.2949944290614397
1117	40-44	0.3406256581429403
1117	45-49	0.43542575522359783
1117	50-54	0.30608257601736
1117	55-59	0.5688963617223806
1117	60-64	0.5631576083605481
1117	65-69	0.4903595213874752
1117	70-74	0.42458216631435874
1117	75-79	0.45432825085039497
1117	80-84	0.6043307983062292
1117	85-89	0.6187989186611702
1117	90-94	0.5664054658008411
1117	95-99	-0.003539076138331154
1117	100-104	0.5979048404140741
1117	105-109	0.5753409565207264
1117	110-114	0.6531255020368363
1117	115-119	0.9780642376351736
1117	120-124	0.9129966491733654
1117	125-129	0.7097280027985065
1117	130-134	0.792436404252058
1117	135-139	0.6064656464441427
1117	140-144	0.7224317494413839
1117	145-149	-0.5259829308263164
1117	150-151	0.16470241758794657
2117	1	-30.961392359284766
2117	2	-10.524213354265317
2117	3	-1.881091022097646
2117	4	-0.36442080258812837
2117	5	0.03864989402440244
2117	6	1.2381786441965659
2117	7	-0.10845216710919203
2117	8	0.24951750709002596
2117	9	0.2576948145047453
2117	10-14	0.40176178708357924
2117	15-19	0.3947963783335382
2117	20-24	0.32792801791760695
2117	25-29	0.39659570648935016
2117	30-34	0.44244767674124574
2117	35-39	0.3484248196518678
2117	40-44	0.20749887483027152
2117	45-49	0.4871897770473126
2117	50-54	0.38719630041339315
2117	55-59	0.5600630105877045
2117	60-64	0.6466475389789252
2117	65-69	0.5422004340948021
2117	70-74	0.5273591206288657
2117	75-79	0.6190384959203286
2117	80-84	0.6248720534531742
2117	85-89	0.7889825848795411
2117	90-94	0.6562976114869912
2117	95-99	0.05905795023888771
2117	100-104	0.9381435127684412
2117	105-109	0.7925686014908351
2117	110-114	0.8893044883196239
2117	115-119	1.1704846275062906
2117	120-124	1.004894333618843
2117	125-129	0.8290050541121374
2117	130-134	1.0769048817144835
2117	135-139	1.0290733645232564
2117	140-144	1.0610395285536498
2117	145-149	1.1974479720067066
2117	150-151	1.6958285120977905
1116	1	0.3510119286172255
1116	2	0.29386844598788997
1116	3	-0.06612822754050285
1116	4	-0.26486166853384674
1116	5	0.10524808648526829
1116	6	1.2452020288686896
1116	7	-0.03960354570071445
1116	8	0.2568551535112604
1116	9	0.11220174400442318
1116	10-14	0.24795646520983894
1116	15-19	0.30256151068327597
1116	20-24	0.13177876438764713
1116	25-29	0.1212706507767507
1116	30-34	0.2086675745134201
1116	35-39	0.20173687988218347
1116	40-44	0.11636437305039493
1116	45-49	0.34619211124191196
1116	50-54	0.2575385365178562
1116	55-59	0.44795769298657007
1116	60-64	0.44458167556303607
1116	65-69	0.34901929564648526
1116	70-74	0.3057113209108593
1116	75-79	0.3347626584324459
1116	80-84	0.3918017105424454
1116	85-89	0.4001580475020603
1116	90-94	0.44317518770249364
1116	95-99	-0.43548986139744983
1116	100-104	0.6022618483392677
1116	105-109	0.4477873141757627
1116	110-114	0.5362686298734545
1116	115-119	1.0724080330917118
1116	120-124	0.7130114503712974
1116	125-129	0.4283391452716572
1116	130-134	0.7206747409207281
1116	135-139	0.8915280804733641
1116	140-144	0.6030934286600242
1116	145-149	0.6033885309990907
1116	150-151	0.7663111259459967
2118	1	0.8865294011064293
2118	2	0.3676207096921118
2118	3	0.07188066128182413
2118	4	0.07860182701237761
2118	5	0.09321135800121993
2118	6	1.3073658742516088
2118	7	0.5529708804011193
2118	8	0.1610628039890898
2118	9	0.12001022869860378
2118	10-14	0.23196966489880566
2118	15-19	0.22923214624280774
2118	20-24	0.14204906096878034
2118	25-29	0.2824792410584607
2118	30-34	0.22927850943730732
2118	35-39	0.17523890364311967
2118	40-44	0.17112860458910717
2118	45-49	0.4179528394214316
2118	50-54	0.19756886485014036
2118	55-59	0.4353752042910557
2118	60-64	0.46812934159193986
2118	65-69	0.479545198912831
2118	70-74	0.4540989394628099
2118	75-79	0.5553700987336754
2118	80-84	0.5456595118506868
2118	85-89	0.4218939513799782
2118	90-94	0.5889702954804008
2118	95-99	0.6195873385611179
2118	100-104	0.7917430840877628
2118	105-109	0.4416069242507561
2118	110-114	0.75858289379984
2118	115-119	1.0706482908950221
2118	120-124	0.8048154369868925
2118	125-129	0.620290180616017
2118	130-134	0.7486501936927752
2118	135-139	0.6972480788259006
2118	140-144	0.6501138260235777
2118	145-149	0.5180416085759632
2118	150-151	1.3995140349697408
1119	1	0.7265088752831375
1119	2	0.304573832333908
1119	3	0.02461572723611738
1119	4	-0.0643375916238611
1119	5	0.07435174859556071
1119	6	1.2436645853506434
1119	7	0.1742682331736134
1119	8	0.3250774135456709
1119	9	0.10341570426381708
1119	10-14	0.17258845810114565
1119	15-19	0.19173943592858222
1119	20-24	-0.02668817830798531
1119	25-29	0.12833079105996603
1119	30-34	-0.03659996561475509
1119	35-39	0.13671458959802862
1119	40-44	-0.10028145910131059
1119	45-49	0.04385932618529864
1119	50-54	0.11820516537233772
1119	55-59	0.23906502287900366
1119	60-64	0.2556240441184272
1119	65-69	0.11324715188454348
1119	70-74	-7.378500569430457E-4
1119	75-79	0.18603447133774154
1119	80-84	0.08285073404272225
1119	85-89	0.19331162169902427
1119	90-94	0.35193180956172654
1119	95-99	0.22377726279668053
1119	100-104	0.5820693843081202
1119	105-109	0.2671857222826972
1119	110-114	0.37250495604138223
1119	115-119	0.7468983437188186
1119	120-124	0.4524015397971226
1119	125-129	0.3138678919867317
1119	130-134	0.6197332930920965
1119	135-139	0.46516935625654554
1119	140-144	0.4084654816727067
1119	145-149	0.6055586480278095
1119	150-151	1.1570225622419201
2119	1	0.9084069554484699
2119	2	0.3709163569436882
2119	3	0.07864955548433983
2119	4	0.06423803245836979
2119	5	0.08282512652561991
2119	6	1.2972829026400348
2119	7	0.5447539023964367
2119	8	0.29112298922414226
2119	9	0.03681865199862955
2119	10-14	0.163753955463406
2119	15-19	0.12127704402125516
2119	20-24	0.1085447580057135
2119	25-29	0.17570730707671345
2119	30-34	0.1520781221646459
2119	35-39	0.18479290579968932
2119	40-44	-0.15780949521377607
2119	45-49	0.19843549413003103
2119	50-54	0.10481744578784458
2119	55-59	0.349319006671891
2119	60-64	0.3074772012403031
2119	65-69	0.2678489901398393
2119	70-74	0.23574140158824264
2119	75-79	0.2688059946966419
2119	80-84	0.2960174279025125
2119	85-89	0.548135790949047
2119	90-94	0.3215203231854744
2119	95-99	0.40871531685660756
2119	100-104	0.7324778250542963
2119	105-109	0.5055519592979749
2119	110-114	0.5177797697684667
2119	115-119	1.0318527136541036
2119	120-124	0.5592139616168836
2119	125-129	0.5914744618458627
2119	130-134	0.605759507265148
2119	135-139	0.4092128652574729
2119	140-144	0.3803860777460173
2119	145-149	0.5823109186537891
2119	150-151	0.5535744739186441
1118	1	0.9075968458182757
1118	2	0.39905619824578054
1118	3	0.10053668581328168
1118	4	0.0961639894469073
1118	5	0.12325711454378308
1118	6	1.2440202959844058
1118	7	0.5236502222939095
1118	8	0.37671312514866884
1118	9	0.10118718198950205
1118	10-14	0.11421871773598014
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2111	1	0.9306240173784914
2111	2	0.3373504096618376
2111	3	0.09041326820936746
2111	4	0.11303986771879693
2111	5	0.02669768033419473
2111	6	1.0180732690257912
2111	7	0.4205136051235243
2111	8	-0.14270402876323374
2111	9	-0.07174913645544478
2111	10-14	-0.13757669852596877
2111	15-19	-0.11602295081844716
2111	20-24	-0.07046001701806404
2111	25-29	0.07201594465436756
2111	30-34	-0.05412875745675905
2111	35-39	-0.24661032795002313
2111	40-44	-0.22443663304017036
2111	45-49	-0.13279644797850665
2111	50-54	-0.3261503194198596
2111	55-59	-0.3312997875131032
2111	60-64	-0.26173794837561104
2111	65-69	-0.3661245200261902
2111	70-74	-0.22990948911326825
2111	75-79	-0.4738500327501072
2111	80-84	-0.11015132567649744
2111	85-89	-0.5912587815257524
2111	90-94	-0.7034111526430706
2111	95-99	-0.3793507809755141
2111	100-104	-0.36889716736808964
2111	105-109	-0.4145820980794568
2111	110-114	-0.6389174127620834
2111	115-119	-2.3913788717429654
2111	120-124	-1.3204422627225512
2111	125-129	-0.9676874866604308
2111	130-134	-0.6910724741217251
2111	135-139	-0.6230924176292447
2111	140-144	-1.0230936625960965
2111	145-149	-1.3094148283486078
2111	150-151	-2.4983011169473386
2110	1	0.9585265459357117
2110	2	0.34298222213949714
2110	3	0.06064834035269939
2110	4	0.0662204981435508
2110	5	0.06336515801782383
2110	6	1.1276484915410023
2110	7	0.13161137797410305
2110	8	-0.04741844098430903
2110	9	-0.15498930659261845
2110	10-14	-0.01198826594507807
2110	15-19	-0.13634406165922286
2110	20-24	-0.19640733757537987
2110	25-29	-0.1696556806288143
2110	30-34	-0.035563904718593164
2110	35-39	-0.2549294944251699
2110	40-44	-0.17947421563550847
2110	45-49	-0.1639334816588729
2110	50-54	-0.23217333950523766
2110	55-59	-0.31316064086550455
2110	60-64	-0.06630736321389463
2110	65-69	-0.26492148270713045
2110	70-74	-0.1776092184687812
2110	75-79	-0.4148360460794862
2110	80-84	-0.31601103565061095
2110	85-89	-0.4285699023321854
2110	90-94	-0.45776592466516064
2110	95-99	-0.7538619530368251
2110	100-104	-0.18993014013607024
2110	105-109	-0.27444505869257085
2110	110-114	-0.6803366025301969
2110	115-119	-6.358919430805152
2110	120-124	-3.017129474575924
2110	125-129	-0.49037301457281757
2110	130-134	-0.8481689676751856
2110	135-139	-0.8836003183005872
2110	140-144	-0.7691139643047791
2110	145-149	-0.918309522896962
2110	150-151	-1.399352075616079
2109	1	0.9586280176892572
2109	2	0.352365131869135
2109	3	0.07696618603249306
2109	4	0.06527807600505042
2109	5	0.048391232127322326
2109	6	1.0223022211185224
2109	7	0.4234351042389406
2109	8	-0.016099042244469786
2109	9	-0.23219356097017396
2109	10-14	-0.0625552813251602
2109	15-19	-0.1566278778956871
2109	20-24	-0.4615275712754112
2109	25-29	-0.22100395105181292
2109	30-34	-0.29427525476025096
2109	35-39	-0.6123964083076743
2109	40-44	-0.22858716402511448
2109	45-49	-0.6354508251955053
2109	50-54	-0.29144647642058885
2109	55-59	-0.7789524988029797
2109	60-64	-0.5753272209520475
2109	65-69	-0.6341122312717431
2109	70-74	-0.6704463712798159
2109	75-79	-0.6193976274276878
2109	80-84	-0.6880490351440258
2109	85-89	-0.9278792489134773
2109	90-94	-0.9240261534955749
2109	95-99	-0.49282893960202045
2109	100-104	-0.5209598667123032
2109	105-109	-0.6085061829014862
2109	110-114	-1.3840518887980267
2109	115-119	-5.106241131636978
2109	120-124	-1.8271338148817193
2109	125-129	-1.3036482051938378
2109	130-134	-1.4894305843190239
2109	135-139	-1.6955657745271466
2109	140-144	-1.0021558525159548
2109	145-149	-0.9283209894633089
2109	150-151	-2.186545798103488
2108	1	0.8773746828017792
2108	2	0.09507080628869602
2108	3	0.062019548761654164
2108	4	0.03307925616643104
2108	5	-0.014799129213685092
2108	6	0.7962144400858406
2108	7	-0.11207014234872048
2108	8	-0.464533188955663
2108	9	-0.4537360809930888
2108	10-14	-0.3288366738080839
2108	15-19	-0.4709794821596276
2108	20-24	-0.5432046916599091
2108	25-29	-0.320114677922156
2108	30-34	-4.310229507426875
2108	35-39	-1.120510991644153
2108	40-44	-0.6966107455108173
2108	45-49	-0.36819690629694435
2108	50-54	-0.3320788511080863
2108	55-59	-0.46924441677207085
2108	60-64	-0.7389872975136669
2108	65-69	-0.7223922401255223
2108	70-74	-0.7393192751888549
2108	75-79	-0.9827645509605887
2108	80-84	-0.7722783503865003
2108	85-89	-1.205218078916424
2108	90-94	-1.1636393775115366
2108	95-99	-0.9269839247888569
2108	100-104	-1.1067319797906165
2108	105-109	-0.9367526005491271
2108	110-114	-1.3812985815408538
2108	115-119	-1.4583536120504164
2108	120-124	-1.5232952555953858
2108	125-129	-1.1523369130949845
2108	130-134	-1.41678866494577
2108	135-139	-1.9981956060185126
2108	140-144	-1.8579053570577457
2108	145-149	-1.7925132891533906
2108	150-151	-2.240711014053211
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	10.0
18	29.0
19	81.0
20	203.0
21	512.0
22	1120.0
23	1778.0
24	2444.0
25	3088.0
26	4262.0
27	5447.0
28	6898.0
29	8922.0
30	11627.0
31	15185.0
32	20456.0
33	27797.0
34	39704.0
35	62867.0
36	120495.0
37	420783.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.10414004252611657	0.08700995720197731	99.60709566200983	0.2017543382620849
2	52.13092568759868	0.04431412612277932	32.32768571466479	15.49707447161375
3	0.06328694060049622	0.10640697350439825	0.1021613087261679	99.72814477716894
4	0.3148425787106447	28.297355746905307	0.16796911278873838	71.21983256159531
5	67.28211115681097	32.36231441801223	0.18813602048533257	0.16743840469145957
6	33.119104164731795	24.487402316540845	27.323373711374398	15.070119807352961
7	0.6319406668347243	47.19573841397885	28.30651046158337	23.865810457603057
8	67.23952183200436	0.27119183770946387	32.079181648113995	0.4101046821721882
9	51.88812673309363	15.360549813588781	0.6757240848602247	32.07559936845736
10-14	18.372238659431346	39.189422987621235	24.622732881346938	17.81560547160048
15-19	31.184885433389496	21.364583195128102	18.329092091122583	29.121439280359816
20-24	35.2150561887198	22.303724244072654	11.357232887980787	31.123986679226757
25-29	24.80771118865346	45.78195857823301	5.047272823765109	24.363057409348425
30-34	22.129943877618715	17.072561064600443	39.24050364198432	21.556991415796524
35-39	43.45849199294158	18.528824083533454	18.49390349073251	19.518780432792454
40-44	14.409043265977632	32.872139151663106	26.734844966897082	25.983972615462182
45-49	18.147987952926194	25.692905759509628	21.954252962014568	34.204853325549614
50-54	29.48858314205729	24.96166960767404	18.81280598638734	26.736941263881338
55-59	24.17929973066564	41.97590585238354	27.111647715965027	6.73314670098579
60-64	36.663066696740124	20.185933581881628	17.658303591567048	25.4926961298112
65-69	21.03477464807419	32.20989505247376	26.68787730028791	20.067452999164136
70-74	25.77171591195553	13.310477504610526	28.848681853763384	32.06912472967056
75-79	27.795491634713613	16.47585941542503	29.711825503177614	26.01682344668374
80-84	28.605697151424287	18.85775696222685	30.529514004059916	22.007031882288945
85-89	31.87661036738268	21.176792134905998	37.13900571837975	9.807591779331574
90-94	30.036406575473322	33.07555956535006	26.65380584044261	10.234228018733996
95-99	16.27716230380385	31.122898727627334	27.90694033514216	24.69299863342665
100-104	10.03468177415717	37.37381751602075	31.37111090472463	21.22038980509745
105-109	17.897095699937644	23.662045083652863	33.692746547080446	24.748112669329053
110-114	42.48689814384843	28.605750222234015	9.440952090326519	19.466399543591038
115-119	7.894574836475568	38.97026707884996	19.22253917289143	33.91261891178305
120-124	6.732615992888512	20.16388266043969	43.07232224595667	30.03117910071513
125-129	20.340873810882172	29.678948136551192	21.112390707301216	28.867787345265423
130-134	21.65978957423943	25.35821469796075	45.7907948680527	7.191200859747117
135-139	27.57076329092091	4.50064348356795	43.7421554709371	24.18643775457404
140-144	14.396624696501306	35.56288227567632	19.051200063684973	30.989292964137398
145-149	20.43677276406045	20.56093192341882	33.539186159132825	25.463109153387908
150-151	36.67604250971859	18.290589218665005	33.42346525852118	11.609903013095222
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.5
22	0.5
23	0.0
24	0.0
25	0.5
26	11.0
27	12.5
28	5.5
29	6.0
30	6.5
31	11.0
32	23.0
33	86.0
34	391.5
35	3472.5
36	74944.5
37	81877.5
38	11127.0
39	1280.5
40	386.5
41	280.0
42	292.0
43	387.0
44	593.0
45	1499.5
46	5866.0
47	47943.0
48	51413.0
49	21254.5
50	113086.0
51	110896.0
52	12393.5
53	2085.0
54	777.5
55	361.5
56	240.0
57	221.5
58	285.5
59	558.5
60	1598.0
61	8369.0
62	91607.5
63	94937.5
64	11498.5
65	1210.5
66	268.5
67	86.0
68	29.0
69	13.5
70	6.5
71	3.0
72	3.5
73	1.5
74	0.5
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.4096801156943655
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	753710.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	1.3813632551857162
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.4149034955806	1.0141265007180043
2	8.94319217206476	0.24707594101109587
3	3.503746673297868	0.145198407299186
4	1.9805652017445057	0.1094351997675738
5	1.5155731800251595	0.10467785507158607
6	1.2431533304471643	0.10303477987448754
7	0.6453379463857174	0.06240122884199679
8	0.5104126975818013	0.05640522763357757
9	0.46223921396786755	0.05746682387530262
>10	4.266001681997748	1.2992304677543758
>50	1.646694585153603	1.6895177641776176
>100	1.5215072775103466	4.0069013536381535
>500	0.12518730764325636	1.1598224853054597
>1k	0.16370647922579676	5.987073106850342
>5k	0.01925958579127021	1.4504764743400314
>10k+	0.03851917158254042	82.5071563838412
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	202355	26.8478592562126	No Hit
TGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	175677	23.30830160141168	No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	153152	20.319751628610476	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	89066	11.817011848058272	No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC	5649	0.7494925103819772	No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	5255	0.6972177628000159	No Hit
TGCAGTTGGTCGACAGTCCTCGGTCCGCAACGGTGTGGACATGGCAGAGG	4918	0.6525056056042775	No Hit
NGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	4813	0.6385745180507092	No Hit
TGCAGGTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	4477	0.5939950378792904	No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	4099	0.5438431226864443	No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG	3286	0.4359767019145294	No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	2711	0.35968741293070283	No Hit
TGCAGATGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	2591	0.34376617001233895	No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG	2583	0.34270475381778137	No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	2525	0.3350094864072388	No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC	2310	0.3064839261785037	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG	2296	0.3046264478380279	No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	2296	0.3046264478380279	No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	1579	0.20949702140080403	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA	1239	0.1643868331321065	No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC	1147	0.15218054689469424	No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	1074	0.14249512411935625	No Hit
TGCAGCTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	1064	0.14116835387615925	No Hit
TTCCATATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	989	0.13121757705218187	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	5.307080972787942E-4
6	0.0	0.0	5.307080972787942E-4
7	0.0	0.0	5.307080972787942E-4
8	0.0	0.0	5.307080972787942E-4
9	0.0	0.0	5.307080972787942E-4
10-11	0.0	0.0	5.307080972787942E-4
12-13	0.0	0.0	5.307080972787942E-4
14-15	0.0	0.0	5.307080972787942E-4
16-17	0.0	0.0	5.307080972787942E-4
18-19	0.0	0.0	5.307080972787942E-4
20-21	0.0	0.0	5.307080972787942E-4
22-23	0.0	0.0	0.0011940932188772872
24-25	0.0	0.0	0.0017248013161560813
26-27	0.0	0.0	0.0017248013161560813
28-29	0.0	0.0	0.0017248013161560813
30-31	0.0	0.0	0.0020564938769553277
32-33	0.0	0.0	0.0029188945350333683
34-35	0.0	0.0	0.0037149566809515596
36-37	0.0	0.0	0.003980310729590957
38-39	0.0	0.0	0.0043783418025500525
40-41	0.0	0.0	0.005240742460628093
42-43	0.0	0.0	0.006633851215984928
44-45	0.0	0.0	0.023417494792426795
46-47	0.0	0.0	0.06361863316129546
48-49	0.0	0.0	0.29407862440461185
50-51	0.0	0.0	0.44068673627787874
52-53	0.0	0.0	0.4434729537885924
54-55	0.0	0.0	0.4479176341033023
56-57	0.0	0.0	0.45494951639224634
58-59	0.0	0.0	0.4690132809701344
60-61	0.0	0.0	0.4719985140173276
62-63	0.0	0.0	0.4814185827440262
64-65	0.0	0.0	0.4814849212561861
66-67	0.0	0.0	0.48161759828050577
68-69	0.0	0.0	0.4843374772790596
70-71	0.0	0.0	0.48447015430337925
72-73	0.0	0.0	0.4847355083520187
74-75	0.0	0.0	0.48519987793713765
76-77	0.0	0.0	0.485465231985777
78-79	0.0	0.0	0.48752172586273235
80-81	0.0	0.0	0.48997625081264673
82-83	0.0	0.0	0.49024160486128615
84-85	0.0	0.0	0.49024160486128615
86-87	0.0	0.0	0.49024160486128615
88-89	0.0	0.0	0.49024160486128615
90-91	0.0	0.0	0.49024160486128615
92-93	0.0	0.0	0.4903742818856059
94-95	0.0	0.0	0.4913030210558438
96-97	0.0	0.0	0.49223176022608167
98-99	0.0	0.0	0.49223176022608167
100-101	0.0	0.0	0.4926297912990407
102-103	0.0	0.0	0.49455360815167637
104-105	0.0	0.0	0.4960793939313529
106-107	0.0	0.0	0.4960793939313529
108-109	0.0	0.0	0.4962120709556726
110-111	0.0	0.0	0.4962784094678324
112-113	0.0	0.0	0.4963447479799923
114-115	0.0	0.0	0.4963447479799923
116-117	0.0	0.0	0.4963447479799923
118-119	0.0	0.0	0.4987992729299067
120-121	0.0	0.0	0.5080203261201258
122-123	0.0	0.0	0.9325868039431612
124-125	0.0	0.0	1.1296121850579135
126-127	0.0	0.0	1.2223534250573829
128-129	0.0	0.0	1.2670655822531212
130-131	0.0	0.0	1.2673309363017606
132-133	0.0	0.0	1.2673309363017606
134-135	0.0	0.0	1.2673309363017606
136-137	0.0	0.0	1.2673309363017606
138-139	0.0	0.0	1.2673309363017606
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGTTG	10	0.006594062	148.5633	1
TTCCGCA	25	3.2097887E-8	148.5633	1
TGCCGCG	10	0.006594062	148.5633	1
TACACGC	10	0.006594062	148.5633	1
AGCAGTT	10	0.006594062	148.5633	1
TCCCGGA	235	0.0	148.5633	1
TCCCGCA	10715	0.0	148.5633	1
TGCAGAC	20	1.8877654E-6	148.5633	1
TGCGGTT	25	3.2097887E-8	148.5633	1
TGAAAGA	10	0.006594062	148.5633	1
TCCCACA	15	1.1165004E-4	148.56328	1
TGCCGTT	15	1.1165004E-4	148.56328	1
TCCCGTA	60	0.0	148.56328	1
TCCCGCG	30	5.493348E-10	148.56328	1
TGAGTTG	15	1.1165004E-4	148.56328	1
TGCAGTT	19800	0.0	148.48825	1
TTCCAGA	22685	0.0	148.07213	1
TGCAGGT	510	0.0	145.65028	1
TGTTGGT	15	1.2307023E-4	144.97586	4
CCAACGG	15	1.2307023E-4	144.97586	3
>>END_MODULE