FastQCFastQC Report
Wed 17 Jun 2015
000000000-AG1W8 l01n01 mis07.3410000000d1b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG1W8 l01n01 mis07.3410000000d1b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753710
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC20235526.8478592562126No Hit
TGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG17567723.30830160141168No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG15315220.319751628610476No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG8906611.817011848058272No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC56490.7494925103819772No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC52550.6972177628000159No Hit
TGCAGTTGGTCGACAGTCCTCGGTCCGCAACGGTGTGGACATGGCAGAGG49180.6525056056042775No Hit
NGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG48130.6385745180507092No Hit
TGCAGGTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG44770.5939950378792904No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG40990.5438431226864443No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG32860.4359767019145294No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG27110.35968741293070283No Hit
TGCAGATGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG25910.34376617001233895No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG25830.34270475381778137No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG25250.3350094864072388No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC23100.3064839261785037No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG22960.3046264478380279No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG22960.3046264478380279No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC15790.20949702140080403No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA12390.1643868331321065No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC11470.15218054689469424No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG10740.14249512411935625No Hit
TGCAGCTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG10640.14116835387615925No Hit
TTCCATATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC9890.13121757705218187No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTG100.006594062148.56331
TTCCGCA253.2097887E-8148.56331
TGCCGCG100.006594062148.56331
TACACGC100.006594062148.56331
AGCAGTT100.006594062148.56331
TCCCGGA2350.0148.56331
TCCCGCA107150.0148.56331
TGCAGAC201.8877654E-6148.56331
TGCGGTT253.2097887E-8148.56331
TGAAAGA100.006594062148.56331
TCCCACA151.1165004E-4148.563281
TGCCGTT151.1165004E-4148.563281
TCCCGTA600.0148.563281
TCCCGCG305.493348E-10148.563281
TGAGTTG151.1165004E-4148.563281
TGCAGTT198000.0148.488251
TTCCAGA226850.0148.072131
TGCAGGT5100.0145.650281
TGTTGGT151.2307023E-4144.975864
CCAACGG151.2307023E-4144.975863
Produced by FastQC (version 0.11.2)