##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AG1W8 l01n01 mis06.3410000000d1a7.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	849454
Sequences flagged as poor quality	0
Sequence length	151
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.987038733115625	34.0	34.0	34.0	34.0	34.0
2	33.50616866834461	34.0	34.0	34.0	34.0	34.0
3	33.87797808945511	34.0	34.0	34.0	34.0	34.0
4	33.760469666397476	34.0	34.0	34.0	34.0	34.0
5	33.7994947342646	34.0	34.0	34.0	34.0	34.0
6	36.41148314093524	38.0	38.0	38.0	34.0	38.0
7	37.12418329891907	38.0	38.0	38.0	36.0	38.0
8	37.17651809279843	38.0	38.0	38.0	36.0	38.0
9	37.41825101771256	38.0	38.0	38.0	37.0	38.0
10-14	37.29720408638961	38.0	38.0	38.0	36.6	38.0
15-19	37.18586668613015	38.0	38.0	38.0	36.2	38.0
20-24	37.43219503351564	38.0	38.0	38.0	36.8	38.0
25-29	37.140703793260144	38.0	38.0	38.0	35.8	38.0
30-34	36.9511702811453	38.0	38.0	38.0	35.6	38.0
35-39	36.54851257395927	38.0	37.6	38.0	34.2	38.0
40-44	36.37972297499335	38.0	37.6	38.0	34.2	38.0
45-49	36.09809948508101	38.0	37.0	38.0	32.6	38.0
50-54	36.16543332540668	38.0	37.2	38.0	33.0	38.0
55-59	35.98061366477761	38.0	37.0	38.0	32.0	38.0
60-64	36.07642367921041	38.0	37.0	38.0	32.4	38.0
65-69	35.98052372465137	38.0	37.0	38.0	31.8	38.0
70-74	35.51538258693231	38.0	37.0	38.0	30.0	38.0
75-79	35.59408208096024	38.0	36.6	38.0	29.8	38.0
80-84	35.33418501767018	38.0	36.4	38.0	28.4	38.0
85-89	35.56692534263185	38.0	36.8	38.0	30.4	38.0
90-94	35.21476901633285	38.0	36.4	38.0	28.4	38.0
95-99	34.85327116006282	37.8	35.8	38.0	26.6	38.0
100-104	34.85017575995875	38.0	36.0	38.0	26.4	38.0
105-109	35.16361309735431	38.0	36.0	38.0	27.6	38.0
110-114	35.40850028371166	38.0	36.8	38.0	29.2	38.0
115-119	34.80692962773735	38.0	35.6	38.0	26.4	38.0
120-124	34.66075596795118	38.0	35.4	38.0	25.6	38.0
125-129	34.964463055091855	38.0	36.0	38.0	27.0	38.0
130-134	33.9237658543017	37.6	34.4	38.0	23.2	38.0
135-139	33.85982642968307	38.0	34.2	38.0	22.4	38.0
140-144	33.62978713385304	37.2	33.6	38.0	22.2	38.0
145-149	31.794477629159434	36.6	29.0	38.0	12.4	38.0
150-151	31.661084649669085	35.0	29.5	37.5	20.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7865342668900368
1101	2	0.38540243073428115
1101	3	0.07146443481757814
1101	4	0.06856387412259579
1101	5	0.11744281155807812
1101	6	1.2533158742459918
1101	7	0.4004358594872457
1101	8	0.2763272428350021
1101	9	0.19141103205499377
1101	10-14	0.22219957751873665
1101	15-19	0.2662659603299531
1101	20-24	0.10206071202343026
1101	25-29	0.28835362998443514
1101	30-34	0.1986250103337852
1101	35-39	0.34972589735993864
1101	40-44	0.4180128867665758
1101	45-49	0.44616712140096126
1101	50-54	0.38651211150261844
1101	55-59	0.3463793508352211
1101	60-64	0.413240504900223
1101	65-69	0.36612435338934546
1101	70-74	0.39388667837630464
1101	75-79	0.5078905476240578
1101	80-84	0.583931308009106
1101	85-89	0.6135712319556816
1101	90-94	0.5288818015834096
1101	95-99	0.5913994154433553
1101	100-104	0.8518346537205659
1101	105-109	0.636726537942323
1101	110-114	0.4028933587558683
1101	115-119	0.6495106310558114
1101	120-124	0.464823760416472
1101	125-129	0.5044668060980371
1101	130-134	0.7741191091183452
1101	135-139	0.6034909203103638
1101	140-144	0.7575646902656672
1101	145-149	0.7226109736565647
1101	150-151	0.4461039552645154
1102	1	0.8710790900858214
1102	2	0.3304175140724368
1102	3	0.08316197952503046
1102	4	0.09687443173588406
1102	5	0.11704004664112233
1102	6	1.2053148686901167
1102	7	0.5443099262735132
1102	8	0.23161690080132047
1102	9	0.19688805618833527
1102	10-14	0.26049979020466196
1102	15-19	0.2569690898513386
1102	20-24	0.1520658121449614
1102	25-29	0.29436215188964354
1102	30-34	0.13715574127886043
1102	35-39	0.4331924969895482
1102	40-44	0.5695093564696876
1102	45-49	0.49456271721090417
1102	50-54	0.3859221974450975
1102	55-59	0.5683460806807048
1102	60-64	0.6240953720800277
1102	65-69	0.5472619698602017
1102	70-74	0.543471759978253
1102	75-79	0.46584131155715625
1102	80-84	0.7271920942235894
1102	85-89	0.6122659263824417
1102	90-94	0.7076133905063813
1102	95-99	0.7090195757535582
1102	100-104	0.914601234541216
1102	105-109	0.5510875035653413
1102	110-114	0.48561128948103516
1102	115-119	0.7225142047579922
1102	120-124	0.6962705310629218
1102	125-129	0.5779057710265505
1102	130-134	0.9691132132331646
1102	135-139	0.8435669519094091
1102	140-144	0.7941860586897675
1102	145-149	0.8976231271376705
1102	150-151	1.1184710627360097
1103	1	0.8316551227267013
1103	2	0.38932509940848803
1103	3	0.076972292750078
1103	4	0.1134078166484187
1103	5	0.13454072407434126
1103	6	1.289664929681912
1103	7	0.5166624397104158
1103	8	0.30898825919862105
1103	9	0.21339352264102018
1103	10-14	0.22611238599188255
1103	15-19	0.28956234070052744
1103	20-24	0.13553994514448675
1103	25-29	0.2981537342002909
1103	30-34	0.30156047135935893
1103	35-39	0.23186795766693535
1103	40-44	0.31795516500562115
1103	45-49	0.329167702936914
1103	50-54	0.2755990640815327
1103	55-59	0.4851191811534008
1103	60-64	0.6700644117853685
1103	65-69	0.44894165753839843
1103	70-74	0.3795770435923984
1103	75-79	0.39020472961602337
1103	80-84	0.5766491896636694
1103	85-89	0.6191204739437239
1103	90-94	0.5492082462108314
1103	95-99	0.7018344590297545
1103	100-104	0.8338898794831806
1103	105-109	0.6147344390581821
1103	110-114	0.5475199450247246
1103	115-119	0.8557407667091539
1103	120-124	0.994769047096419
1103	125-129	0.7293248407944262
1103	130-134	0.8631681323106832
1103	135-139	0.8317840115481658
1103	140-144	1.070745008152798
1103	145-149	1.2436542261122625
1103	150-151	1.2630788311843197
2116	1	0.7433085736643434
2116	2	0.35262521358205845
2116	3	-0.06228607705793365
2116	4	-0.10561822193449899
2116	5	0.07451290925317267
2116	6	1.2012658128209281
2116	7	0.19817775722913922
2116	8	0.27022568673344693
2116	9	0.15884843699240037
2116	10-14	0.22004816474231603
2116	15-19	0.2896311030701
2116	20-24	0.16728425537196756
2116	25-29	0.3038112466920566
2116	30-34	0.3495999138086816
2116	35-39	0.4256498326588698
2116	40-44	0.3474077036613892
2116	45-49	0.5170501797840217
2116	50-54	0.46617507804059244
2116	55-59	0.6236685312494075
2116	60-64	0.7408474137571233
2116	65-69	0.6546336987736865
2116	70-74	0.6182669433815704
2116	75-79	0.5386903896739028
2116	80-84	0.7525999198027193
2116	85-89	0.713204217213665
2116	90-94	0.7223544381810498
2116	95-99	0.829920972714909
2116	100-104	1.1641300497659657
2116	105-109	0.6817915461323452
2116	110-114	0.6637393427268563
2116	115-119	0.8638681541633417
2116	120-124	0.6147914391341516
2116	125-129	0.5932119067181318
2116	130-134	0.9741250322492618
2116	135-139	0.9588037493731889
2116	140-144	1.0643340976600726
2116	145-149	1.2364912825368748
2116	150-151	1.7727690131795661
1117	1	0.9361439679351449
1117	2	0.3609663142676567
1117	3	0.08211003719464571
1117	4	0.08147140295417188
1117	5	0.10345924202725598
1117	6	1.2727751647130603
1117	7	0.4732642337510242
1117	8	0.3248001393940214
1117	9	0.13807335880105143
1117	10-14	0.22966973933997537
1117	15-19	0.20090598448851438
1117	20-24	0.12560153617729952
1117	25-29	0.3070213380361153
1117	30-34	0.24477918429895595
1117	35-39	0.249602275750064
1117	40-44	0.3693323499879497
1117	45-49	0.46591821764727115
1117	50-54	0.4060984401649108
1117	55-59	0.5719131096375207
1117	60-64	0.61040767805423
1117	65-69	0.4250668063359839
1117	70-74	0.5093624507450158
1117	75-79	0.447814853726598
1117	80-84	0.6704598589066819
1117	85-89	0.6571276621897368
1117	90-94	0.45991031133500826
1117	95-99	-0.11238520595490797
1117	100-104	0.678972113603244
1117	105-109	0.6785611561076834
1117	110-114	0.5923973513848679
1117	115-119	0.9329325506918451
1117	120-124	0.9119608088394102
1117	125-129	0.6662304033729924
1117	130-134	0.7633538895163952
1117	135-139	0.4670396283267735
1117	140-144	0.8206099111607656
1117	145-149	-0.5853716392303525
1117	150-151	0.27672104201198167
2117	1	-30.922925192569423
2117	2	-10.516239303380676
2117	3	-1.717591660577284
2117	4	-0.28578462911101354
2117	5	0.05103478250109106
2117	6	1.2708679487687604
2117	7	0.043398032059428715
2117	8	0.3600726396521381
2117	9	0.3004633619486796
2117	10-14	0.39285684859578396
2117	15-19	0.4248209268877474
2117	20-24	0.28986239942339864
2117	25-29	0.4194824507888697
2117	30-34	0.5500373579245093
2117	35-39	0.49416356896269065
2117	40-44	0.35951911976895445
2117	45-49	0.7224467411748066
2117	50-54	0.40135814886159693
2117	55-59	0.7627471223742148
2117	60-64	0.9146005583817285
2117	65-69	0.6936231253317331
2117	70-74	0.8095330677242529
2117	75-79	0.7258123000216159
2117	80-84	0.8873607199077753
2117	85-89	0.8274879306114897
2117	90-94	0.7850822319728721
2117	95-99	-0.01921124637397753
2117	100-104	0.9991151160557905
2117	105-109	0.9432607397536685
2117	110-114	0.8931983318199315
2117	115-119	1.1320573297766785
2117	120-124	1.1345544172890385
2117	125-129	0.7925990931154345
2117	130-134	1.0255127105315083
2117	135-139	0.8986815701561568
2117	140-144	1.182485110505361
2117	145-149	1.2079253564071593
2117	150-151	1.7439018283663081
1116	1	0.3340883820912097
1116	2	0.23652562689424173
1116	3	-0.09282220759406101
1116	4	-0.32882246866815734
1116	5	0.08347061821088175
1116	6	1.2384521558896253
1116	7	-0.21534009746518734
1116	8	0.3021741304465948
1116	9	0.10939592871635995
1116	10-14	0.23243812772052053
1116	15-19	0.26775924943353147
1116	20-24	0.08807170321824742
1116	25-29	-4.1746577194601286E-4
1116	30-34	0.14327800042794792
1116	35-39	0.24182698883603138
1116	40-44	0.16386497188336335
1116	45-49	0.3673818089391787
1116	50-54	0.2590085191093294
1116	55-59	0.4641715576347778
1116	60-64	0.6031388478132698
1116	65-69	0.4294083359012646
1116	70-74	0.46649636102275593
1116	75-79	0.34919282841163835
1116	80-84	0.48034158989733555
1116	85-89	0.3997940979211947
1116	90-94	0.6645632098015852
1116	95-99	-0.6618351143352967
1116	100-104	0.7122744543809603
1116	105-109	0.6158982237684185
1116	110-114	0.6021628799739247
1116	115-119	0.9637984199759515
1116	120-124	0.9098979012994377
1116	125-129	0.48884960235582753
1116	130-134	0.919288104471164
1116	135-139	0.7811167305097015
1116	140-144	0.6865887057970355
1116	145-149	1.0270019483416561
1116	150-151	0.8642363693528061
2118	1	0.7074065609850777
2118	2	0.33790044388099005
2118	3	0.05190320916926794
2118	4	-0.015622120090057479
2118	5	0.08461733878888111
2118	6	1.303204632295298
2118	7	0.4870946854825604
2118	8	0.2756256580101919
2118	9	0.1057514549854659
2118	10-14	0.26756216732741933
2118	15-19	0.22085861173836463
2118	20-24	0.07776619049845124
2118	25-29	0.22221710623534818
2118	30-34	0.28729179538682814
2118	35-39	0.17185118265168597
2118	40-44	0.19557454471922853
2118	45-49	0.3052244068208054
2118	50-54	0.056574126069968145
2118	55-59	0.3090566028933992
2118	60-64	0.39508306751885414
2118	65-69	0.356992288051714
2118	70-74	0.39895260842742175
2118	75-79	0.4031121290719497
2118	80-84	0.39785627482211083
2118	85-89	0.226569146123623
2118	90-94	0.5288856952336332
2118	95-99	0.5573496262099127
2118	100-104	0.7550727879124963
2118	105-109	0.4353575378025951
2118	110-114	0.5533624526343246
2118	115-119	0.8948121233063588
2118	120-124	0.6618434077027047
2118	125-129	0.4678049597945986
2118	130-134	0.5249060995232213
2118	135-139	0.41005998129292465
2118	140-144	0.4268166072515882
2118	145-149	0.3702538233136323
2118	150-151	1.2081047674726726
1119	1	0.8478720344669739
1119	2	0.3451158540796371
1119	3	0.0320078150695835
1119	4	-0.027067800663104435
1119	5	0.08219745463269135
1119	6	1.3152881855954703
1119	7	0.2097126406011114
1119	8	0.3416347513834097
1119	9	0.09478179883564053
1119	10-14	0.2072247836173844
1119	15-19	0.18527636479854692
1119	20-24	0.054457047917381374
1119	25-29	0.19222939699928787
1119	30-34	0.13025354094411057
1119	35-39	0.24241890991321924
1119	40-44	-0.0036948044601814445
1119	45-49	0.18707126211013048
1119	50-54	0.11730819679935678
1119	55-59	0.32122755801120917
1119	60-64	0.3358150400072475
1119	65-69	0.26149723385213264
1119	70-74	0.2924696038060688
1119	75-79	0.33151197017312484
1119	80-84	0.2485870003132078
1119	85-89	0.4667948899724479
1119	90-94	0.5253118158697916
1119	95-99	0.39411177902010053
1119	100-104	0.5863154842044338
1119	105-109	0.5186336237543046
1119	110-114	0.43380273975732564
1119	115-119	0.8317489677460586
1119	120-124	0.7292228540508319
1119	125-129	0.3719238591215941
1119	130-134	0.47904366647466645
1119	135-139	0.5763107830673917
1119	140-144	0.5453391469146851
1119	145-149	0.7977514954608651
1119	150-151	1.2139487606418164
2119	1	0.8732833382362628
2119	2	0.3456557217784493
2119	3	0.04931811653678153
2119	4	0.038664825192149976
2119	5	-0.01964806405472075
2119	6	1.2687367305288149
2119	7	0.42763755493538724
2119	8	0.20970579588180271
2119	9	0.11781522060373106
2119	10-14	0.13477457577205598
2119	15-19	0.08815718993741228
2119	20-24	0.09396610432870034
2119	25-29	0.13975265125905167
2119	30-34	0.09254815040620912
2119	35-39	0.11696104908856597
2119	40-44	-0.22545087535832664
2119	45-49	0.14563802164201434
2119	50-54	0.17126606919792664
2119	55-59	0.30224919229452496
2119	60-64	0.24842214550248087
2119	65-69	0.24609181411806702
2119	70-74	0.23273115215857842
2119	75-79	0.038872577154670296
2119	80-84	0.3678692002852699
2119	85-89	0.4582796157487792
2119	90-94	0.39902787532841444
2119	95-99	0.44825871711899623
2119	100-104	0.8254734772705277
2119	105-109	0.45689997640663904
2119	110-114	0.4888213065765967
2119	115-119	0.9007578363610804
2119	120-124	0.5455142392408305
2119	125-129	0.47400918211256027
2119	130-134	0.5683356557489816
2119	135-139	0.38403671545923146
2119	140-144	0.5563297481391345
2119	145-149	0.6360875156875991
2119	150-151	0.4317152801827113
1118	1	0.8299410494687933
1118	2	0.39910548490309594
1118	3	0.06863459462707056
1118	4	0.10324658629336625
1118	5	0.1415793884215617
1118	6	1.3379189685823647
1118	7	0.45337669841534023
1118	8	0.43868737366646826
1118	9	0.16839430513699227
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2104	150-151	-0.4246875745165113
2111	1	0.8770748074305743
2111	2	0.3334602479478477
2111	3	0.07882664469260448
2111	4	0.08683116618686881
2111	5	0.0862741927684425
2111	6	0.9693337357863498
2111	7	0.39443943025619177
2111	8	-0.14039072798319552
2111	9	-0.14629213238507077
2111	10-14	-0.21197502846538896
2111	15-19	-0.09024011151637978
2111	20-24	-0.049890551597826516
2111	25-29	0.0108603251762176
2111	30-34	-0.04905937678682193
2111	35-39	-0.23602477215924722
2111	40-44	-0.2092728670966295
2111	45-49	-0.3854109429960033
2111	50-54	-0.5222184570499877
2111	55-59	-0.456760424760418
2111	60-64	-0.5337067185190989
2111	65-69	-0.4996443238870256
2111	70-74	-0.39807243478012566
2111	75-79	-0.609668631714726
2111	80-84	-0.32797162710139816
2111	85-89	-0.6477897774021955
2111	90-94	-0.9287737654300727
2111	95-99	-0.4902786150928762
2111	100-104	-0.6623715427948511
2111	105-109	-0.5429865307641961
2111	110-114	-0.6861862062875232
2111	115-119	-2.0793834310016877
2111	120-124	-1.4738677528507864
2111	125-129	-1.0202900507018242
2111	130-134	-0.9655779526928967
2111	135-139	-0.7485500357938122
2111	140-144	-1.3542626738296377
2111	145-149	-1.7946964818627364
2111	150-151	-2.6902313122353334
2110	1	0.9633654997735377
2110	2	0.2955925167969582
2110	3	0.06616625923484776
2110	4	0.001436804652854562
2110	5	0.11255396036162324
2110	6	1.2475998547533251
2110	7	0.1799072152997283
2110	8	-0.07026866531523979
2110	9	-0.07354228095540094
2110	10-14	-0.052150576452291375
2110	15-19	-0.17583082610572376
2110	20-24	-0.1735387913658002
2110	25-29	-0.1305508057442779
2110	30-34	-0.03243453958753406
2110	35-39	-0.25222937204289764
2110	40-44	-0.18084813037160785
2110	45-49	-0.12716878327950099
2110	50-54	-0.22140728915663743
2110	55-59	-0.2482149726836198
2110	60-64	-0.09021602074509616
2110	65-69	-0.245390596051152
2110	70-74	-0.21705939923500495
2110	75-79	-0.2258316632387931
2110	80-84	-0.35243780267653335
2110	85-89	-0.2748613073580515
2110	90-94	-0.39339384837879265
2110	95-99	-0.6719457987529722
2110	100-104	0.04888790539039434
2110	105-109	-0.2805960158700813
2110	110-114	-0.7309155576226729
2110	115-119	-6.084575131116267
2110	120-124	-3.3316794398264094
2110	125-129	-0.453907072422858
2110	130-134	-0.8813461265781513
2110	135-139	-0.6087331245735896
2110	140-144	-0.9360796289159623
2110	145-149	-0.7304675174893269
2110	150-151	-1.0230061882352466
2109	1	0.98510531438437
2109	2	0.3986414225861594
2109	3	0.09250408567301349
2109	4	0.06864022486769272
2109	5	0.07599572432953039
2109	6	1.16229195581208
2109	7	0.45847909788041363
2109	8	-0.0685401683177389
2109	9	-0.11888370076665922
2109	10-14	0.012442634337332947
2109	15-19	-0.051488145559240195
2109	20-24	-0.3209213927260066
2109	25-29	-0.08685803019526617
2109	30-34	-0.17932913829667996
2109	35-39	-0.30119057681949357
2109	40-44	-0.10609424526451505
2109	45-49	-0.3056727231542311
2109	50-54	-0.3231397973038952
2109	55-59	-0.42539720785671165
2109	60-64	-0.4176972717054568
2109	65-69	-0.37135908816122054
2109	70-74	-0.3793465691713749
2109	75-79	-0.3562380914456966
2109	80-84	-0.481318266093929
2109	85-89	-0.42652905086414705
2109	90-94	-0.5840888448705712
2109	95-99	-0.3099752010978136
2109	100-104	-0.3590815703770147
2109	105-109	-0.4769898071039407
2109	110-114	-1.2650601305679032
2109	115-119	-4.423349805086925
2109	120-124	-1.6079263365849812
2109	125-129	-0.8536158012401245
2109	130-134	-1.072369173410486
2109	135-139	-1.028521298840154
2109	140-144	-0.7563630876131526
2109	145-149	-0.5831869844630688
2109	150-151	-1.6026972433095672
2108	1	0.8470748074305803
2108	2	0.05842607938292588
2108	3	0.047825565685293725
2108	4	0.0035114203530355326
2108	5	0.003389646550964187
2108	6	0.878921315213276
2108	7	-0.25570923297363635
2108	8	-0.5553086035376822
2108	9	-0.46667218273874767
2108	10-14	-0.32187528023374057
2108	15-19	-0.4445798789303481
2108	20-24	-0.5784341315043093
2108	25-29	-0.27894473416919396
2108	30-34	-4.437212116266508
2108	35-39	-1.376707424119445
2108	40-44	-0.9999821345705513
2108	45-49	-0.6113840877028807
2108	50-54	-0.573654495894246
2108	55-59	-0.6388167729201086
2108	60-64	-0.9570564367783021
2108	65-69	-0.8605173606931587
2108	70-74	-1.0408663443832893
2108	75-79	-1.1725714011668416
2108	80-84	-1.1478594103408142
2108	85-89	-1.672958462212172
2108	90-94	-1.504775204106494
2108	95-99	-1.43595371400189
2108	100-104	-0.8742823448569581
2108	105-109	-1.2614397138357702
2108	110-114	-1.5003681988543676
2108	115-119	-1.5677486150323858
2108	120-124	-1.6622192694555125
2108	125-129	-1.1913285352959946
2108	130-134	-1.786820969117791
2108	135-139	-1.9957868179542224
2108	140-144	-2.2875980053246607
2108	145-149	-2.0973733791172613
2108	150-151	-2.1365542950911056
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	5.0
17	16.0
18	39.0
19	133.0
20	351.0
21	1142.0
22	4050.0
23	7216.0
24	7081.0
25	7088.0
26	8360.0
27	9973.0
28	11818.0
29	14124.0
30	17649.0
31	22410.0
32	29900.0
33	37487.0
34	48904.0
35	75810.0
36	143859.0
37	402039.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.10667490460801798	0.08929799707006031	99.6227762988635	0.1812507994584197
2	51.84212447054225	0.03814214777963256	32.55408768455973	15.565645697118386
3	0.0641588596910486	0.10135922604402357	0.10088833533069479	99.73359357893423
4	0.3103169800836773	28.103464107532606	0.1460938438102593	71.44012506857345
5	67.06460856032228	32.581517068611134	0.18564866372987826	0.16822570733671277
6	33.2804366098694	24.39649468952998	27.178046133163186	15.145022567437435
7	0.6135705994674225	47.53229721680044	28.102640048784277	23.75149213494786
8	67.01551820345775	0.2731166137307023	32.325352520560266	0.3860126622512814
9	51.6116234663678	15.403541569054946	0.6525368059953806	32.33229815858187
10-14	18.42647159234049	39.100386836721	24.67987672081125	17.793264850127258
15-19	31.177438684142988	21.422843379394294	18.266392294344367	29.133325642118347
20-24	35.4222359303741	22.3911359532123	11.307051352986743	30.879576763426858
25-29	24.75328858301921	45.797653551575486	5.002319136763144	24.446738728642163
30-34	22.297381612188534	17.479204288872616	38.68115283464437	21.542261264294478
35-39	31.12281536139685	30.89709389796269	36.64768192274096	1.3324088178994977
40-44	26.285943676761782	26.88515210947267	26.483882588109537	20.34502162565601
45-49	17.846758035161407	31.935808177959018	10.30824506094503	39.90918872593454
50-54	35.42362505797842	24.691672533180135	18.739708094846808	21.144994313994637
55-59	11.498491972490564	48.05790543101804	27.936015369872884	12.507587226618512
60-64	42.66618321886765	14.747167003745936	23.674689859603934	18.911959917782482
65-69	20.82646029096337	32.23774330334545	26.458242588768787	20.477553816922402
70-74	31.650542583824432	13.607117042241251	22.456636851436336	32.28570352249798
75-79	34.25741711734832	10.6885834901007	17.960336875216314	37.093662517334664
80-84	41.002620506819675	30.47218566279045	17.414221370433243	11.110972459956631
85-89	20.587106541378343	26.819133231463976	36.981096092313415	15.612664134844264
90-94	23.74654778245791	32.24690212771969	32.834432470740026	11.172117619082375
95-99	22.22863156804253	35.6921269427185	23.078259682101677	19.00098180713729
100-104	4.919042114110947	36.51449048447591	36.85459130217764	21.711876099235507
105-109	23.022671033393216	23.7437459827136	28.186858852863132	25.04672413103005
110-114	23.427825403141313	34.58951279292346	28.58968231358025	13.39297949035498
115-119	7.465477824579082	38.72614644230294	13.305652807568155	40.502722925549826
120-124	13.374073228214831	15.476411906942577	42.48696221337471	28.66255265146788
125-129	14.26495136876158	23.386669554796374	21.46757799716053	40.88080107928151
130-134	12.951825525572897	32.427159092781956	46.97257297040217	7.6484424112429865
135-139	22.28584478971198	15.2146437594031	44.319668869650386	18.179842581234535
140-144	24.66810445297803	24.75514860133686	25.24779446562145	25.328952480063666
145-149	21.60585505512953	14.093994495287562	33.644717665700554	30.655432783882354
150-151	34.67898202845593	21.725896870224872	30.331248072291146	13.263873029028058
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.5
28	1.0
29	1.0
30	6.0
31	15.0
32	33.0
33	98.0
34	414.0
35	3535.5
36	78739.5
37	85601.5
38	11133.0
39	1455.5
40	724.5
41	799.0
42	1117.5
43	1642.5
44	2624.0
45	5606.5
46	14649.5
47	82999.5
48	168967.5
49	116075.0
50	24918.0
51	7140.5
52	2469.5
53	1148.5
54	496.0
55	300.0
56	223.5
57	210.5
58	305.5
59	592.5
60	1660.0
61	9052.5
62	103512.5
63	107125.0
64	12426.5
65	1238.5
66	275.0
67	72.5
68	22.5
69	12.0
70	7.0
71	4.0
72	1.5
73	1.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.4448645836031146
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	849454.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	1.7945150714799525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.85482331771287	1.2715004833068488
2	10.799965525655107	0.38761401814503993
3	4.41260175896076	0.23755441082682094
4	2.511188247206286	0.1802546062774002
5	1.498741160223382	0.13447568001341048
6	1.1276228691816503	0.12141217402151631
7	0.7678209103808401	0.09645063371131128
8	0.5715621537700488	0.08205415193823157
9	0.5747600898381765	0.09282740793298012
>10	4.045399366317808	1.5382671896738023
>50	1.205756208731556	1.6050965687103398
>100	1.2786315839843396	4.511057270064271
>500	0.15237578461989537	1.9118256367252668
>1k	0.1391257163920784	6.196164948376809
>5k	0.03312517056954248	3.1150833488884677
>10k+	0.026500136455633976	78.5183614713875
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	198282	23.342288105065137	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAAGGCGCCAA	186785	21.98883047227984	No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	172641	20.323760909949215	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	102736	12.094357081136824	No Hit
NGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	5554	0.6538317554570348	No Hit
TGCAGTTGGTCGACAGTCCTCGGTCCGCAACGGTGTGGACATGGCAGAGG	5333	0.6278150435456187	No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTTTAATTAAGGCGCCAA	5258	0.6189858426707038	No Hit
TGCAGGTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	5048	0.5942640802209419	No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAAGGCGCCAA	5009	0.5896728957659861	No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	4538	0.5342255142715203	No Hit
TTCCAGATCTCGATGCCGACAGGGAAAGCAATAGAATCCAAAGTACCTGT	4500	0.5297520524948968	No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG	3960	0.4661818061955091	No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	3049	0.3589364462348756	No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG	2955	0.34787051447164885	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	2920	0.34375022073002187	No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	2881	0.3391590362750661	No Hit
TGCAGATGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	2860	0.3366868600300899	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG	2720	0.320205685063582	No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	2630	0.3096106440136841	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAATGCGCCAA	2583	0.3040776781320707	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAAGGCGCCAC	2368	0.2787673022906479	No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTTTAATTAAGGCGCCAA	1927	0.22685160114614802	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTAATTAAGGCGCCAAG	1919	0.22590981971949037	No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAAGGCGCCAA	1834	0.21590339206125345	No Hit
TTCCAGATCTCGATGCCGACAGGCGGAGAATTTTTTAATTAAGGCGCCAA	1762	0.2074273592213351	No Hit
TTCCAGATCTCGATGCCGACAGGGGAAAGCAATAGAATCCAAAGTACCTG	1740	0.2048374602980267	No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC	1353	0.1592787837834656	No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	1303	0.15339264986685563	No Hit
TGCAGCTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	1189	0.13997226453698494	No Hit
TTCCATATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAAGGCGCCAA	1127	0.13267345848038858	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAAGGCACCAA	976	0.11489733405222649	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAAGGCTCCAA	904	0.10642130121230814	No Hit
TTCCAGATCTCGATTCCGACAGGCAGAGAATTTTTTAATTAAGGCGCCAA	900	0.10595041049897935	No Hit
TTCCAGATCTCGATGCCCACAGGCAGAGAATTTTTTAATTAAGGCGCCAA	898	0.10571496514231495	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	8.240587483253949E-4
6	0.0	0.0	8.240587483253949E-4
7	0.0	0.0	8.240587483253949E-4
8	0.0	0.0	8.240587483253949E-4
9	0.0	0.0	8.240587483253949E-4
10-11	0.0	0.0	8.240587483253949E-4
12-13	0.0	0.0	9.417814266575943E-4
14-15	0.0	0.0	0.0015892561574846903
16-17	0.0	0.0	0.002119008209979587
18-19	0.0	0.0	0.002413314905810085
20-21	0.0	0.0	0.002589898923308384
22-23	0.0	0.0	0.0034728190107998786
24-25	0.0	0.0	0.0044734617766235725
26-27	0.0	0.0	0.004591184454955772
28-29	0.0	0.0	0.004591184454955772
30-31	0.0	0.0	0.00482662981162017
32-33	0.0	0.0	0.005415243203281166
34-35	0.0	0.0	0.007710835430759053
36-37	0.0	0.0	0.038848483849625755
38-39	0.0	0.0	0.07045702298182127
40-41	0.0	0.0	0.08458374438168517
42-43	0.0	0.0	0.13137850901873438
44-45	0.0	0.0	0.14974324683855747
46-47	0.0	0.0	0.5500592145072011
48-49	0.0	0.0	1.401429624205666
50-51	0.0	0.0	1.5180339370937097
52-53	0.0	0.0	1.5196820545903604
54-55	0.0	0.0	1.5200940839645232
56-57	0.0	0.0	1.5273928900211196
58-59	0.0	0.0	1.5276871967169499
60-61	0.0	0.0	1.5318663517977429
62-63	0.0	0.0	1.5339264986685566
64-65	0.0	0.0	1.5339264986685566
66-67	0.0	0.0	1.5345739733993837
68-69	0.0	0.0	1.5366341202701972
70-71	0.0	0.0	1.5367518429485294
72-73	0.0	0.0	1.5368695656268614
74-75	0.0	0.0	1.5368695656268614
76-77	0.0	0.0	1.5369284269660275
78-79	0.0	0.0	1.5397537712460003
80-81	0.0	0.0	1.5404012459768275
82-83	0.0	0.0	1.5404012459768275
84-85	0.0	0.0	1.5404601073159936
86-87	0.0	0.0	1.5405189686551597
88-89	0.0	0.0	1.5411664433859866
90-91	0.0	0.0	1.5421082248126443
92-93	0.0	0.0	1.5438740649876275
94-95	0.0	0.0	1.5482298040859188
96-97	0.0	0.0	1.5622388028074505
98-99	0.0	0.0	1.5969081315762832
100-101	0.0	0.0	1.7387050976274172
102-103	0.0	0.0	15.248618524369771
104-105	0.0	0.0	27.01570655974308
106-107	0.0	0.0	27.018767349379722
108-109	0.0	0.0	27.02118066428553
110-111	0.0	0.0	27.022416752408017
112-113	0.0	0.0	27.023005365799676
114-115	0.0	0.0	27.023711701869672
116-117	0.0	0.0	27.02477120597466
118-119	0.0	0.0	27.02600729409715
120-121	0.0	0.0	27.028302886324624
122-123	0.0	0.0	27.19517478286052
124-125	0.0	0.0	28.11288192179918
126-127	0.0	0.0	28.938588787621224
128-129	0.0	0.0	28.94524011894699
130-131	0.0	0.0	29.21888648473019
132-133	0.0	0.0	29.220240295531013
134-135	0.0	0.0	29.22041687954851
136-137	0.0	0.0	29.22041687954851
138-139	0.0	0.0	29.220475740887675
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGGTT	10	0.006587412	148.61456	1
TCCCGCG	20	1.8846677E-6	148.61456	1
AGCAGTT	10	0.006587412	148.61456	1
TTCTAGA	15	1.1150175E-4	148.61455	1
TGCCAGT	15	1.1150175E-4	148.61455	1
CTCCAGA	25	3.203604E-8	148.61455	1
TTTCAGA	15	1.1150175E-4	148.61455	1
CCCCGCA	15	1.1150175E-4	148.61455	1
TGCAGGT	585	0.0	148.61455	1
TGCGTTG	25	3.203604E-8	148.61455	1
TCCCGCA	12235	0.0	148.55383	1
TGCAGTT	22185	0.0	148.44708	1
TTCCAGA	25905	0.0	148.29903	1
GTTGATG	10	0.0070942566	144.97552	8
CTTAAGT	10	0.0070942566	144.97552	145
AGTCCGT	40	0.0	144.97552	145
CGAACGG	10	0.0070942566	144.97552	4
ATGATGG	20	2.132212E-6	144.97552	9
GGAGAAA	40	0.0	144.97552	8
TTAGAGT	10	0.0070942566	144.97552	145
>>END_MODULE