FastQCFastQC Report
Wed 17 Jun 2015
000000000-AG1W8 l01n01 mis06.3410000000d1a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG1W8 l01n01 mis06.3410000000d1a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849454
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG19828223.342288105065137No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAAGGCGCCAA18678521.98883047227984No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG17264120.323760909949215No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG10273612.094357081136824No Hit
NGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG55540.6538317554570348No Hit
TGCAGTTGGTCGACAGTCCTCGGTCCGCAACGGTGTGGACATGGCAGAGG53330.6278150435456187No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTTTAATTAAGGCGCCAA52580.6189858426707038No Hit
TGCAGGTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG50480.5942640802209419No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAAGGCGCCAA50090.5896728957659861No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG45380.5342255142715203No Hit
TTCCAGATCTCGATGCCGACAGGGAAAGCAATAGAATCCAAAGTACCTGT45000.5297520524948968No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG39600.4661818061955091No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG30490.3589364462348756No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG29550.34787051447164885No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC29200.34375022073002187No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG28810.3391590362750661No Hit
TGCAGATGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG28600.3366868600300899No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG27200.320205685063582No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG26300.3096106440136841No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAATGCGCCAA25830.3040776781320707No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAAGGCGCCAC23680.2787673022906479No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTTTAATTAAGGCGCCAA19270.22685160114614802No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTAATTAAGGCGCCAAG19190.22590981971949037No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAAGGCGCCAA18340.21590339206125345No Hit
TTCCAGATCTCGATGCCGACAGGCGGAGAATTTTTTAATTAAGGCGCCAA17620.2074273592213351No Hit
TTCCAGATCTCGATGCCGACAGGGGAAAGCAATAGAATCCAAAGTACCTG17400.2048374602980267No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC13530.1592787837834656No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG13030.15339264986685563No Hit
TGCAGCTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG11890.13997226453698494No Hit
TTCCATATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAAGGCGCCAA11270.13267345848038858No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAAGGCACCAA9760.11489733405222649No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAAGGCTCCAA9040.10642130121230814No Hit
TTCCAGATCTCGATTCCGACAGGCAGAGAATTTTTTAATTAAGGCGCCAA9000.10595041049897935No Hit
TTCCAGATCTCGATGCCCACAGGCAGAGAATTTTTTAATTAAGGCGCCAA8980.10571496514231495No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGGTT100.006587412148.614561
TCCCGCG201.8846677E-6148.614561
AGCAGTT100.006587412148.614561
TTCTAGA151.1150175E-4148.614551
TGCCAGT151.1150175E-4148.614551
CTCCAGA253.203604E-8148.614551
TTTCAGA151.1150175E-4148.614551
CCCCGCA151.1150175E-4148.614551
TGCAGGT5850.0148.614551
TGCGTTG253.203604E-8148.614551
TCCCGCA122350.0148.553831
TGCAGTT221850.0148.447081
TTCCAGA259050.0148.299031
GTTGATG100.0070942566144.975528
CTTAAGT100.0070942566144.97552145
AGTCCGT400.0144.97552145
CGAACGG100.0070942566144.975524
ATGATGG202.132212E-6144.975529
GGAGAAA400.0144.975528
TTAGAGT100.0070942566144.97552145
Produced by FastQC (version 0.11.2)