##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AG1W8 l01n01 mis05.3410000000d19a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1063509 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.033947056395384 34.0 34.0 34.0 34.0 34.0 2 33.534712917333096 34.0 34.0 34.0 34.0 34.0 3 33.886826533672966 34.0 34.0 34.0 34.0 34.0 4 33.7818485786204 34.0 34.0 34.0 34.0 34.0 5 33.81186807069804 34.0 34.0 34.0 34.0 34.0 6 36.448215294840004 38.0 38.0 38.0 34.0 38.0 7 37.169946845771875 38.0 38.0 38.0 36.0 38.0 8 37.21886415629769 38.0 38.0 38.0 36.0 38.0 9 37.4539228158859 38.0 38.0 38.0 37.0 38.0 10-14 37.31221851437083 38.0 38.0 38.0 36.6 38.0 15-19 37.23790809480691 38.0 38.0 38.0 36.2 38.0 20-24 37.47135510841939 38.0 38.0 38.0 37.2 38.0 25-29 37.20206016122101 38.0 38.0 38.0 36.4 38.0 30-34 37.027882979833734 38.0 38.0 38.0 36.0 38.0 35-39 36.71875292075572 38.0 38.0 38.0 35.0 38.0 40-44 36.534193692766124 38.0 37.8 38.0 35.0 38.0 45-49 36.252603033918845 38.0 37.4 38.0 32.8 38.0 50-54 36.294748422439305 38.0 37.2 38.0 33.8 38.0 55-59 36.143844762949826 38.0 37.2 38.0 32.4 38.0 60-64 36.244723081798085 38.0 37.0 38.0 33.4 38.0 65-69 36.10855272498869 38.0 37.2 38.0 32.4 38.0 70-74 35.75434622556086 38.0 37.0 38.0 31.8 38.0 75-79 35.83440158945528 38.0 37.0 38.0 32.0 38.0 80-84 35.56773962420628 38.0 36.6 38.0 29.4 38.0 85-89 35.90351656638543 38.0 37.0 38.0 31.8 38.0 90-94 35.50426070677352 38.0 36.6 38.0 29.6 38.0 95-99 35.20909197759492 38.0 36.4 38.0 28.4 38.0 100-104 35.03991860905737 38.0 36.2 38.0 27.2 38.0 105-109 35.37381103497948 38.0 36.4 38.0 29.2 38.0 110-114 35.64077671180968 38.0 37.0 38.0 30.2 38.0 115-119 35.13182022907187 38.0 36.0 38.0 27.6 38.0 120-124 35.1534328341368 38.0 36.4 38.0 27.4 38.0 125-129 35.2705086651829 38.0 36.4 38.0 28.4 38.0 130-134 34.22907601158053 38.0 34.8 38.0 23.6 38.0 135-139 34.29769940827957 38.0 34.6 38.0 23.8 38.0 140-144 33.774038583594496 37.6 33.8 38.0 20.8 38.0 145-149 32.43198054741427 37.0 30.4 38.0 14.4 38.0 150-151 32.13861565816556 35.0 30.0 37.5 20.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7702793576114857 1101 2 0.3622588843663266 1101 3 0.04818143036253986 1101 4 0.09321087594294397 1101 5 0.1263314025505835 1101 6 1.2483254844986362 1101 7 0.42775182161997805 1101 8 0.3298567575312106 1101 9 0.18193142217885594 1101 10-14 0.24852863599787867 1101 15-19 0.24131280072325012 1101 20-24 0.1567343912400716 1101 25-29 0.25314748230732675 1101 30-34 0.1873992991991642 1101 35-39 0.2907389989094966 1101 40-44 0.37525073983240986 1101 45-49 0.4152238445926173 1101 50-54 0.3878791492451157 1101 55-59 0.49525234793476614 1101 60-64 0.45733681597069875 1101 65-69 0.3585252495508655 1101 70-74 0.3826517319259466 1101 75-79 0.44954784206026943 1101 80-84 0.5408708817310668 1101 85-89 0.5052280362746728 1101 90-94 0.4502852867078744 1101 95-99 0.5934147933695471 1101 100-104 0.8272983586036844 1101 105-109 0.5165420296146053 1101 110-114 0.5176198106921817 1101 115-119 0.5502313685933657 1101 120-124 0.471532881685512 1101 125-129 0.46677753375946907 1101 130-134 0.7356477014969087 1101 135-139 0.622310473284017 1101 140-144 0.5572165487693113 1101 145-149 0.8270425852651897 1101 150-151 0.3112059533788951 1102 1 0.9024566330291819 1102 2 0.32356568077718606 1102 3 0.04947262578096456 1102 4 0.04024444016792472 1102 5 0.0856389409227063 1102 6 1.082560750206845 1102 7 0.529063432399596 1102 8 0.13458306599969916 1102 9 0.1678250170116371 1102 10-14 0.2267614427215321 1102 15-19 0.26312036766164226 1102 20-24 0.1360200364226145 1102 25-29 0.2596750655170581 1102 30-34 0.1840454667102449 1102 35-39 0.344432770015203 1102 40-44 0.3760461009748113 1102 45-49 0.3297205031007593 1102 50-54 0.2002737873180891 1102 55-59 0.4080990186692617 1102 60-64 0.5292381945408167 1102 65-69 0.5067889154641421 1102 70-74 0.40353367552927466 1102 75-79 0.33179554285638346 1102 80-84 0.6086475193972589 1102 85-89 0.5378820256114665 1102 90-94 0.5653949392887014 1102 95-99 0.5494359918274228 1102 100-104 0.8514464552902226 1102 105-109 0.5583141439071042 1102 110-114 0.38554118412798744 1102 115-119 0.6344659867994551 1102 120-124 0.5717931775406555 1102 125-129 0.4863693484184566 1102 130-134 0.8815387672625548 1102 135-139 0.8124502347137579 1102 140-144 0.561617506463044 1102 145-149 0.7262723624406604 1102 150-151 0.9737762168817881 1103 1 0.8371828013141283 1103 2 0.37297312123151016 1103 3 0.0514174845041353 1103 4 0.0768786302439679 1103 5 0.10897535199559627 1103 6 1.2896300074263465 1103 7 0.5326958720270483 1103 8 0.2415896827610311 1103 9 0.22023874499247853 1103 10-14 0.17639704997166916 1103 15-19 0.2887781871525945 1103 20-24 0.08540528382465595 1103 25-29 0.28004538398862167 1103 30-34 0.2782974156717728 1103 35-39 0.20872382066055906 1103 40-44 0.3515054580226433 1103 45-49 0.3637768653741844 1103 50-54 0.19918193459209022 1103 55-59 0.35803098774846376 1103 60-64 0.5447840363227883 1103 65-69 0.40745304066022214 1103 70-74 0.35819728414987395 1103 75-79 0.3662883432433901 1103 80-84 0.5145686206978084 1103 85-89 0.5327592177434539 1103 90-94 0.45052074662725516 1103 95-99 0.46820421935493783 1103 100-104 0.7425134361438452 1103 105-109 0.5015724930015466 1103 110-114 0.42882680113650196 1103 115-119 0.7688078289843787 1103 120-124 0.6895246436278271 1103 125-129 0.5224892858335508 1103 130-134 0.6092774063260151 1103 135-139 0.6330322582404122 1103 140-144 0.7796492225818383 1103 145-149 0.8768556306970936 1103 150-151 0.7286517451660615 2116 1 0.7268652647458183 2116 2 0.2864217561765443 2116 3 -0.02015092113935424 2116 4 -0.16649367071586596 2116 5 0.04493367977615037 2116 6 1.1874768704394327 2116 7 0.12545137496459802 2116 8 0.22195504041710734 2116 9 0.1149440239075048 2116 10-14 0.23168929167426455 2116 15-19 0.2648912490555446 2116 20-24 0.15228326618591836 2116 25-29 0.24328786861904206 2116 30-34 0.2404436000572474 2116 35-39 0.24721674873884325 2116 40-44 0.22371705678006748 2116 45-49 0.3537607287271456 2116 50-54 0.2989242854391705 2116 55-59 0.45614319633064326 2116 60-64 0.5196771904380952 2116 65-69 0.38894192011368034 2116 70-74 0.30921837646244654 2116 75-79 0.3098135911041453 2116 80-84 0.4797643421102791 2116 85-89 0.549135538401984 2116 90-94 0.5720322297834741 2116 95-99 0.6094069574195515 2116 100-104 0.8755218670770333 2116 105-109 0.45608770659517717 2116 110-114 0.43342698523064627 2116 115-119 0.6755220997928575 2116 120-124 0.6860925069064265 2116 125-129 0.5203308285052657 2116 130-134 0.8093745154694645 2116 135-139 0.7529155619761241 2116 140-144 0.7544412385573267 2116 145-149 0.8097174681467152 2116 150-151 1.496464299725389 1117 1 0.9121253912327916 1117 2 0.3778958062694002 1117 3 0.04844755800535694 1117 4 0.010156745888060925 1117 5 0.10757171119711728 1117 6 1.2493776885893126 1117 7 0.4706491363459122 1117 8 0.28238143202479904 1117 9 0.1730615262916828 1117 10-14 0.20211166782243595 1117 15-19 0.19404191662568593 1117 20-24 0.12435511781468733 1117 25-29 0.27121570328249334 1117 30-34 0.25947374110025834 1117 35-39 0.22340193671254127 1117 40-44 0.3145210270656662 1117 45-49 0.4293401469874638 1117 50-54 0.35667129894115135 1117 55-59 0.44143626213397624 1117 60-64 0.5245675809415431 1117 65-69 0.4134324586871898 1117 70-74 0.39680464610055566 1117 75-79 0.30673298075430466 1117 80-84 0.5578822836853377 1117 85-89 0.4491286368309275 1117 90-94 0.3513102627102356 1117 95-99 -0.20390962391859802 1117 100-104 0.5298268788724556 1117 105-109 0.6165984855943236 1117 110-114 0.5343389337323714 1117 115-119 0.934007581397033 1117 120-124 0.8067831032021715 1117 125-129 0.6042972241284161 1117 130-134 0.8324150967072228 1117 135-139 0.5381992165462179 1117 140-144 0.6953120285989911 1117 145-149 -0.40128708689017145 1117 150-151 0.32253137672182675 2117 1 -30.94635162026146 2117 2 -10.534309593131738 2117 3 -1.6797877016157372 2117 4 -0.3287240650570098 2117 5 0.031231692430992553 2117 6 1.2558526923839324 2117 7 -0.009020317642580267 2117 8 0.19518275945232233 2117 9 0.26849452707820376 2117 10-14 0.39135184665258294 2117 15-19 0.4063110484163275 2117 20-24 0.2607270803928188 2117 25-29 0.3848731490194055 2117 30-34 0.427947509918134 2117 35-39 0.28155502553428136 2117 40-44 0.2625907722051011 2117 45-49 0.5623679270650115 2117 50-54 0.24123728050618354 2117 55-59 0.45005945278955295 2117 60-64 0.6935923460199831 2117 65-69 0.5563796375587415 2117 70-74 0.5423137980371919 2117 75-79 0.5249343992527287 2117 80-84 0.6606610676127005 2117 85-89 0.6123606537756388 2117 90-94 0.5683463012635883 2117 95-99 -0.17280295711394444 2117 100-104 0.7409024297824658 2117 105-109 0.6763709290544639 2117 110-114 0.7256196701648747 2117 115-119 1.02309588913392 2117 120-124 0.8313033423322977 2117 125-129 0.47868473775455556 2117 130-134 0.8423199207057834 2117 135-139 0.5630908111150745 2117 140-144 0.8336364100757194 2117 145-149 0.9862495657018968 2117 150-151 1.3682973124064404 1116 1 0.2594444178896751 1116 2 0.24618573045624714 1116 3 -0.09390081568014352 1116 4 -0.24882313916941 1116 5 0.08701350625703697 1116 6 1.2704708654051586 1116 7 -0.08911486463232166 1116 8 0.20384756066260223 1116 9 0.062245923454803176 1116 10-14 0.2708754242420923 1116 15-19 0.2522086444319953 1116 20-24 0.09786636750898481 1116 25-29 0.12233608723944656 1116 30-34 0.24493167702592444 1116 35-39 0.25785718249390044 1116 40-44 0.2931313229815373 1116 45-49 0.43945111543940385 1116 50-54 0.25552645912701877 1116 55-59 0.5054785096272596 1116 60-64 0.6260364608632756 1116 65-69 0.4737589443805206 1116 70-74 0.46459781068846695 1116 75-79 0.3664847538039524 1116 80-84 0.47862543604532704 1116 85-89 0.36659317583802675 1116 90-94 0.5617483282519373 1116 95-99 -0.5748147963323973 1116 100-104 0.7179764875787384 1116 105-109 0.4924681970152065 1116 110-114 0.5771351553024431 1116 115-119 0.8721296772639917 1116 120-124 0.8097651892867859 1116 125-129 0.5609522742629309 1116 130-134 0.8380613427331483 1116 135-139 0.7566588032422246 1116 140-144 0.8801723245148665 1116 145-149 0.9628927247997296 1116 150-151 0.895176736222929 2118 1 0.8340419145242066 2118 2 0.3822975275802136 2118 3 0.048560137871426434 2118 4 -0.012399362301692918 2118 5 0.09997837592518977 2118 6 1.2517827580152456 2118 7 0.4841516524806764 2118 8 0.21600373717580368 2118 9 0.07770183614378112 2118 10-14 0.24277188058323418 2118 15-19 0.241728476104214 2118 20-24 0.09105803216917963 2118 25-29 0.23993430208217603 2118 30-34 0.33120286687469047 2118 35-39 0.24902198420811317 2118 40-44 0.22313757701274994 2118 45-49 0.31976061006933776 2118 50-54 0.14012473429304606 2118 55-59 0.27821196467273523 2118 60-64 0.41632214067853823 2118 65-69 0.3421921213716601 2118 70-74 0.39159068377391293 2118 75-79 0.30228168391501953 2118 80-84 0.36600110946218933 2118 85-89 0.12706671891427135 2118 90-94 0.42569804027261426 2118 95-99 0.4719392686590069 2118 100-104 0.6344871300012755 2118 105-109 0.36734792420951834 2118 110-114 0.47292500129240267 2118 115-119 0.7647107360565215 2118 120-124 0.44348550261735653 2118 125-129 0.40749721847418385 2118 130-134 0.5964500196884543 2118 135-139 0.43156503144270886 2118 140-144 0.529666304816466 2118 145-149 0.5696027383131792 2118 150-151 1.206803691767746 1119 1 0.7865299774560839 1119 2 0.28710699062016687 1119 3 0.013637885452183696 1119 4 -0.05577380078582195 1119 5 0.08750698428810466 1119 6 1.2553420744420336 1119 7 0.21549844849440802 1119 8 0.3052162164870609 1119 9 0.09232505786398093 1119 10-14 0.17266973757500637 1119 15-19 0.147391503422881 1119 20-24 0.006858971094892752 1119 25-29 0.18042985957809776 1119 30-34 0.1290518889022536 1119 35-39 0.13595481683453414 1119 40-44 0.02003880134542868 1119 45-49 0.16439567111554965 1119 50-54 -0.01474873729608106 1119 55-59 0.2845939227829746 1119 60-64 0.35755196695822633 1119 65-69 0.21696312915794636 1119 70-74 0.18090812307105608 1119 75-79 0.27166690109798 1119 80-84 0.20697645539406295 1119 85-89 0.334613863190647 1119 90-94 0.44810373918883073 1119 95-99 0.3404884650183888 1119 100-104 0.4529347748174928 1119 105-109 0.38481128094415595 1119 110-114 0.44432190141400696 1119 115-119 0.77691416394741 1119 120-124 0.6218048496399291 1119 125-129 0.25021172230015765 1119 130-134 0.42263452871733165 1119 135-139 0.3314944065047314 1119 140-144 0.5476327075961578 1119 145-149 0.62493725190334 1119 150-151 1.123655233035464 2119 1 0.839287609160607 2119 2 0.3143651482183927 2119 3 0.02636263943136896 2119 4 -0.0015195580352838078 2119 5 0.03197510713487617 2119 6 1.220260592146289 2119 7 0.39913565928409867 2119 8 0.2132402801839035 2119 9 0.025612505618127557 2119 10-14 0.08678737287708316 2119 15-19 0.02509006264899938 2119 20-24 0.06197328114983236 2119 25-29 0.03141169438500668 2119 30-34 0.011860710925333251 2119 35-39 0.017606447854518592 2119 40-44 -0.2828502413281626 2119 45-49 0.009396083336277172 2119 50-54 0.06883615893671902 2119 55-59 0.13956585429937007 2119 60-64 0.16023944619104924 2119 65-69 0.0737214254122378 2119 70-74 0.0756602784857634 2119 75-79 -0.0723159755031233 2119 80-84 0.15622666337815616 2119 85-89 0.181915691202164 2119 90-94 0.26373464924726164 2119 95-99 0.24996689338316003 2119 100-104 0.5187657992984 2119 105-109 0.2646383481061463 2119 110-114 0.2461401445542819 2119 115-119 0.6924351484803921 2119 120-124 0.2619387838994598 2119 125-129 0.1942594299782101 2119 130-134 0.3458554715285729 2119 135-139 0.07170498883520082 2119 140-144 0.16553987548990534 2119 145-149 0.27694139409700824 2119 150-151 0.1600172227874097 1118 1 0.8274009429647862 1118 2 0.35507965539063946 1118 3 0.07096810309849388 1118 4 0.07041932435617326 1118 5 0.10632201564161647 1118 6 1.2547575174718588 1118 7 0.3847496305280771 1118 8 0.4037772020140338 1118 9 0.1409583451413141 1118 10-14 0.14643780448069776 1118 15-19 0.14806957000678267 1118 20-24 0.09065713854048596 1118 25-29 0.244257093984956 1118 30-34 0.13080989889287054 1118 35-39 -0.021674017279018187 1118 40-44 -0.03701347140621891 1118 45-49 0.13061130096959062 1118 50-54 0.33946738594549686 1118 55-59 0.17483338149979488 1118 60-64 0.3073722120333784 1118 65-69 -0.0030234538213491646 1118 70-74 0.03428436616874819 1118 75-79 -0.3736393843276815 1118 80-84 -0.5910765652394829 1118 85-89 0.05801930000924216 1118 90-94 0.06571328633513218 1118 95-99 -0.004171102458208509 1118 100-104 0.18090043855535498 1118 105-109 0.2798615118904735 1118 110-114 0.1529257121989147 1118 115-119 0.6498727242698905 1118 120-124 0.4356754260940292 1118 125-129 0.0819063134661846 1118 130-134 0.34524645016924893 1118 135-139 0.24972278447858542 1118 140-144 0.13721257738475146 1118 145-149 0.4109086159248605 1118 150-151 0.7118151022975212 2112 1 0.9141774075640328 2112 2 0.318609932631162 2112 3 0.046678357154043226 2112 4 -0.007627436780140329 2112 5 0.06292709130507035 2112 6 1.2341589582087096 2112 7 0.45278240413318116 2112 8 0.4076588182372234 2112 9 0.06566025011767351 2112 10-14 0.15495077987067418 2112 15-19 0.14242416765921462 2112 20-24 0.09298930166745834 2112 25-29 0.20455192399899857 2112 30-34 0.2793489567902512 2112 35-39 -0.04818547251997529 2112 40-44 0.11668735415632625 2112 45-49 0.0758697345210706 2112 50-54 0.3307893637922703 2112 55-59 0.22452091033719057 2112 60-64 0.3373364530753733 2112 65-69 0.10176121169303087 2112 70-74 0.1042749480073013 2112 75-79 0.16768262874033724 2112 80-84 0.12531662409843847 2112 85-89 0.22920031303335975 2112 90-94 0.16561344584042104 2112 95-99 0.16521292254606124 2112 100-104 0.4053491497277477 2112 105-109 0.27276684915387506 2112 110-114 0.3252323493141702 2112 115-119 0.7480710870454459 2112 120-124 0.4981084910492868 2112 125-129 0.3817709464737078 2112 130-134 0.3694000072921284 2112 135-139 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-4.636386148073363 2108 35-39 -0.9882283595178549 2108 40-44 -0.6637314547989135 2108 45-49 -0.39227506220617414 2108 50-54 -0.42287138182666695 2108 55-59 -0.2996502424092782 2108 60-64 -0.5698624698686388 2108 65-69 -0.565013825486858 2108 70-74 -0.670628737467581 2108 75-79 -0.8437670435885494 2108 80-84 -0.7962885046690431 2108 85-89 -1.047710167005512 2108 90-94 -0.9996575040321147 2108 95-99 -0.9746421833704773 2108 100-104 -0.5262489590749553 2108 105-109 -0.8608416918429569 2108 110-114 -1.0383478036170857 2108 115-119 -1.0392958600185693 2108 120-124 -1.1671911498041752 2108 125-129 -0.8841351071645391 2108 130-134 -1.3298062882683723 2108 135-139 -1.3498705998644027 2108 140-144 -1.3651585796182744 2108 145-149 -1.3633332863068333 2108 150-151 -1.671484638109611 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 11.0 17 33.0 18 87.0 19 210.0 20 474.0 21 1207.0 22 3725.0 23 7745.0 24 8343.0 25 8396.0 26 9540.0 27 11065.0 28 13107.0 29 16168.0 30 19879.0 31 25588.0 32 34168.0 33 43442.0 34 55519.0 35 85108.0 36 167510.0 37 552184.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.10428281731152542 0.08454322585366511 99.64170234236732 0.1694716144674836 2 51.29914274350288 0.0382695397970304 33.33700043911241 15.325587277587685 3 0.06487956378366333 0.09214778624346386 0.09224181459677351 99.7507308353761 4 0.3205426564326207 21.527791490245967 0.13737542418540888 78.014290429136 5 66.28002207785735 33.398212897117 0.15072745035538015 0.17103757467026606 6 33.962100931915 30.22776488022198 20.869781073784992 14.94035311407802 7 0.6116544382793188 48.094656462709764 21.54640910420128 29.747279994809634 8 66.23902571581435 0.27522099013736606 33.1071011152703 0.3786521787779887 9 51.0316320783369 15.244910950447998 0.6234079824430259 33.10004898877207 10-14 17.17288711237987 43.9023083020454 23.607134495335725 15.31767009023901 15-19 32.54885478167087 20.156594819601903 19.270979371119566 28.023571027607662 20-24 34.17319458509519 24.853536735467213 8.701045313203744 32.27222336623385 25-29 23.602301437975605 46.04369121464886 6.243877578845125 24.110129768530403 30-34 20.718978400746963 17.48158219629547 39.21655576022394 22.58288364273363 35-39 28.319591089497127 32.26109040920199 38.080016248099454 1.3393022532014303 40-44 23.76115293805694 27.127800516967888 28.89291957096743 20.218126974007742 45-49 15.316447721645984 33.05969201953157 10.260543164185728 41.36331709463671 50-54 33.90070041720381 25.93542696864813 17.746159176838184 22.417713437309885 55-59 8.968161059285817 46.57243145097973 31.657465992295318 12.801941497439136 60-64 39.91648401659037 14.680834858943365 27.434671450829285 17.96800967363699 65-69 18.278773381325404 34.65287082666907 26.720902220855674 20.347453571149845 70-74 29.892346938295773 13.48889384104883 24.973347663254376 31.645411557401022 75-79 35.624465801417756 13.198271006639342 15.08310696007274 36.09415623187016 80-84 33.74508349247632 33.62292185585641 21.909452576329866 10.722542075337397 85-89 18.355839019698 28.053866963044037 34.82697372565723 18.76332029160073 90-94 18.94171088349981 37.102008539655046 33.23746202429881 10.718818552546335 95-99 20.817764588734086 37.9408354795305 25.847021510866387 15.394378420869028 100-104 4.4331735791610605 38.8754208944165 33.981564801050105 22.70984072537233 105-109 25.02643607153301 25.411444566994735 27.930106844417868 21.63201251705439 110-114 24.230899785521327 31.83297931658312 27.532272881564708 16.403848016330844 115-119 9.185159693053842 42.20624367071647 11.044513962740325 37.56408267348937 120-124 10.575970678198304 15.679340748409276 44.83561493132639 28.90907364206603 125-129 15.423151096981783 23.975387138237664 16.356495337604102 44.24496642717645 130-134 12.611684527352379 37.23962843755906 42.870271901789266 7.278415133299294 135-139 33.54516040766933 12.174170599402544 38.971405037475 15.309263955453128 140-144 12.369711962945306 24.720806311935302 26.319908905331314 36.58957281978808 145-149 26.223266563799648 13.067251899137666 33.17771640860585 27.531765128456836 150-151 39.58335096364958 17.331541152919254 27.07480613704256 16.010301746388606 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 1.0 14 1.0 15 0.5 16 0.0 17 0.0 18 0.0 19 0.5 20 0.5 21 0.0 22 0.5 23 0.5 24 0.0 25 0.0 26 0.5 27 1.0 28 3.5 29 8.0 30 10.0 31 14.0 32 41.0 33 137.5 34 657.0 35 6017.0 36 140623.0 37 150597.5 38 16825.0 39 1847.0 40 869.0 41 919.5 42 1191.0 43 1747.0 44 2696.5 45 4704.5 46 14069.0 47 95123.5 48 206476.0 49 141890.5 50 30366.0 51 13540.5 52 4077.0 53 2313.5 54 1357.0 55 587.0 56 310.5 57 243.0 58 317.0 59 575.5 60 1498.5 61 8287.5 62 98776.0 63 101977.5 64 11313.0 65 1151.0 66 247.0 67 57.0 68 21.5 69 9.0 70 5.0 71 2.0 72 1.5 73 1.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.349486464148399 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 1063509.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 1.5859610545695575 #Duplication Level Percentage of deduplicated Percentage of total 1 71.20558723274951 1.1292928821889603 2 10.405025345601725 0.33003929939866977 3 4.515897929481945 0.21486114727709 4 2.3411106400272814 0.14851641198086715 5 1.594072058870958 0.12640681017734254 6 1.0366647534448306 0.09864659553650722 7 0.8020974420732342 0.08904667135686077 8 0.5790343341434414 0.07346607225680911 9 0.5301072085534609 0.07566564487611342 >10 4.116155362264843 1.4102286153383472 >50 1.1054798818540374 1.2591164026544512 >100 1.3923070944181808 4.442730531330678 >500 0.15536474014966825 1.6658699477320478 >1k 0.15536474014966825 5.803345779913509 >5k 0.041828968501833756 4.04024164362912 >10k+ 0.023902267715333575 79.09252554435263 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG 273037 25.67321950260882 No Hit TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAAGGCGCCAA 244062 22.948747965461504 No Hit TGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG 189575 17.825425078678226 No Hit TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG 127898 12.02603833159851 No Hit NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG 6893 0.6481374393634657 No Hit TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTTTAATTAAGGCGCCAA 6736 0.6333749878938495 No Hit TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG 6725 0.6323406760074434 No Hit NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAAGGCGCCAA 6231 0.5858906694724727 No Hit TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC 5863 0.551288235454519 No Hit NGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG 5125 0.48189531071199204 No Hit TGCAGTTGGTCGACAGTCCTCGGTCCGCAACGGTGTGGACATGGCAGAGG 5059 0.47568943939355474 No Hit TTCCAGATCTCGATGCCGACAGGGAAAGCAATAGAATCCAAAGTACCTGT 4600 0.43253042522442214 No Hit TGCAGGTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG 4564 0.42914540450527455 No Hit TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG 4448 0.4182381155213543 No Hit TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAATGCGCCAA 3635 0.3417930642806032 No Hit TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG 3567 0.3353991362555465 No Hit TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG 3539 0.3327663423628761 No Hit NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG 3408 0.32044862807931107 No Hit TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAAGGCGCCAC 3226 0.30333546777695347 No Hit TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG 3142 0.29543708609894226 No Hit TGCAGATGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG 2743 0.2579197731283891 No Hit TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTTTAATTAAGGCGCCAA 2627 0.24701248414446894 No Hit TTCCAGATCTCGATGCCGACAGGGGAAAGCAATAGAATCCAAAGTACCTG 2565 0.24118272623927017 No Hit TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC 2223 0.20902502940736747 No Hit TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAAGGCGCCAA 2106 0.1980237120701376 No Hit TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAACCCCCAAC 1703 0.16013028568634585 No Hit TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG 1527 0.1435812955038462 No Hit TTCCAGATCTCGATGCCGACGACGGGAAAGCAATAGAATCCAAAGTACCT 1394 0.13107552451366186 No Hit TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAAGGCTCCAA 1370 0.12881884403423008 No Hit TTCCATATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAAGGCGCCAA 1347 0.12665619190810798 No Hit TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG 1162 0.10926094654582143 No Hit TTCCAGATCTCGATTCCGACAGGCAGAGAATTTTTTAATTAAGGCGCCAA 1126 0.10587592582667377 No Hit TGCAGTTGGTCGACAGTCCTCGGTACGTGAGAAAACGAGAAAGTGAGCTG 1071 0.10070436639464264 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 2.8208505992897097E-4 5 0.0 0.0 0.001786538712883483 6 0.0 0.0 0.001786538712883483 7 0.0 0.0 0.001786538712883483 8 0.0 0.0 0.001786538712883483 9 0.0 0.0 0.001786538712883483 10-11 0.0 0.0 0.001786538712883483 12-13 0.0 0.0 0.0020216095961576256 14-15 0.0 0.0 0.0022566804794317678 16-17 0.0 0.0 0.0022566804794317678 18-19 0.0 0.0 0.0024917513627059104 20-21 0.0 0.0 0.0027268222459800526 22-23 0.0 0.0 0.0032439781891831663 24-25 0.0 0.0 0.003573077425766966 26-27 0.0 0.0 0.0036200916024217945 28-29 0.0 0.0 0.0036671057790766226 30-31 0.0 0.0 0.0037611341323862796 32-33 0.0 0.0 0.004184261722279736 34-35 0.0 0.0 0.005077531078721478 36-37 0.0 0.0 0.04259484404927462 38-39 0.0 0.0 0.0735771864648066 40-41 0.0 0.0 0.07668012212402528 42-43 0.0 0.0 0.137892580128612 44-45 0.0 0.0 0.15514678296093404 46-47 0.0 0.0 0.4847631754879366 48-49 0.0 0.0 1.4234012124015876 50-51 0.0 0.0 1.5230712669098239 52-53 0.0 0.0 1.5369404490229983 54-55 0.0 0.0 1.5371285057296178 56-57 0.0 0.0 1.5374576049662014 58-59 0.0 0.0 1.545826128410761 60-61 0.0 0.0 1.5499163617797311 62-63 0.0 0.0 1.5539595809720463 64-65 0.0 0.0 1.5540065951487012 66-67 0.0 0.0 1.5545707652685592 68-69 0.0 0.0 1.5591311404040775 70-71 0.0 0.0 1.5596482963472806 72-73 0.0 0.0 1.5597423247005902 74-75 0.0 0.0 1.5597423247005902 76-77 0.0 0.0 1.5597423247005902 78-79 0.0 0.0 1.5616228917667834 80-81 0.0 0.0 1.5621870618866414 82-83 0.0 0.0 1.5622810902399509 84-85 0.0 0.0 1.562798246183154 86-87 0.0 0.0 1.5634094304796669 88-89 0.0 0.0 1.5636915155395958 90-91 0.0 0.0 1.5643026998361087 92-93 0.0 0.0 1.566042224372337 94-95 0.0 0.0 1.57074364203782 96-97 0.0 0.0 1.5816979451983952 98-99 0.0 0.0 1.6131504293804755 100-101 0.0 0.0 1.8189314805986596 102-103 0.0 0.0 15.581391412766605 104-105 0.0 0.0 27.714386996254852 106-107 0.0 0.0 27.716549648380973 108-109 0.0 0.0 27.71767798862069 110-111 0.0 0.0 27.718430215447167 112-113 0.0 0.0 27.719135428096987 114-115 0.0 0.0 27.720169739983394 116-117 0.0 0.0 27.721345094399766 118-119 0.0 0.0 27.72176822198966 120-121 0.0 0.0 27.722661491346102 122-123 0.0 0.0 27.791161146732186 124-125 0.0 0.0 27.85604071051585 126-127 0.0 0.0 27.856510852282398 128-129 0.0 0.0 27.856557866459053 130-131 0.0 0.0 27.856651894812362 132-133 0.0 0.0 27.857075022402256 134-135 0.0 0.0 27.85721606493222 136-137 0.0 0.0 27.85731009328553 138-139 0.0 0.0 27.857545164168805 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCAGGT 455 0.0 148.46655 1 GGCAGTT 15 1.1195337E-4 148.46654 1 TCCTGCA 15 1.1195337E-4 148.46654 1 TGAGTTG 15 1.1195337E-4 148.46654 1 TGCGGTT 15 1.1195337E-4 148.46654 1 TGCTGGT 10 0.0066073076 148.46654 1 ACCCGCA 10 0.0066073076 148.46654 1 TGCAGCT 120 0.0 148.46654 1 AGCAGTT 15 1.1195337E-4 148.46654 1 TGCACGG 10 0.0066073076 148.46654 1 TGCGTTG 45 0.0 148.46654 1 TGCCGCG 35 9.094947E-12 148.46654 1 TGCAGTT 21140 0.0 148.43143 1 TCCCGCA 14565 0.0 148.41557 1 TTCCAGA 33285 0.0 148.24352 1 TCCCGGA 290 0.0 145.90677 1 GTCGCAG 15 1.230822E-4 144.9765 9 GATGTGA 35 1.0913936E-11 144.9765 8 GATCGAT 15 1.230822E-4 144.9765 6 ATGGAAA 15 1.230822E-4 144.9765 6 >>END_MODULE