FastQCFastQC Report
Wed 17 Jun 2015
000000000-AG1W8 l01n01 mis05.3410000000d19a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG1W8 l01n01 mis05.3410000000d19a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063509
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG27303725.67321950260882No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAAGGCGCCAA24406222.948747965461504No Hit
TGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG18957517.825425078678226No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG12789812.02603833159851No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG68930.6481374393634657No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTTTAATTAAGGCGCCAA67360.6333749878938495No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG67250.6323406760074434No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAAGGCGCCAA62310.5858906694724727No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC58630.551288235454519No Hit
NGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG51250.48189531071199204No Hit
TGCAGTTGGTCGACAGTCCTCGGTCCGCAACGGTGTGGACATGGCAGAGG50590.47568943939355474No Hit
TTCCAGATCTCGATGCCGACAGGGAAAGCAATAGAATCCAAAGTACCTGT46000.43253042522442214No Hit
TGCAGGTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG45640.42914540450527455No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG44480.4182381155213543No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAATGCGCCAA36350.3417930642806032No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG35670.3353991362555465No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG35390.3327663423628761No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG34080.32044862807931107No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAAGGCGCCAC32260.30333546777695347No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG31420.29543708609894226No Hit
TGCAGATGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG27430.2579197731283891No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTTTAATTAAGGCGCCAA26270.24701248414446894No Hit
TTCCAGATCTCGATGCCGACAGGGGAAAGCAATAGAATCCAAAGTACCTG25650.24118272623927017No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC22230.20902502940736747No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAAGGCGCCAA21060.1980237120701376No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAACCCCCAAC17030.16013028568634585No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG15270.1435812955038462No Hit
TTCCAGATCTCGATGCCGACGACGGGAAAGCAATAGAATCCAAAGTACCT13940.13107552451366186No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAAGGCTCCAA13700.12881884403423008No Hit
TTCCATATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAAGGCGCCAA13470.12665619190810798No Hit
TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG11620.10926094654582143No Hit
TTCCAGATCTCGATTCCGACAGGCAGAGAATTTTTTAATTAAGGCGCCAA11260.10587592582667377No Hit
TGCAGTTGGTCGACAGTCCTCGGTACGTGAGAAAACGAGAAAGTGAGCTG10710.10070436639464264No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCAGGT4550.0148.466551
GGCAGTT151.1195337E-4148.466541
TCCTGCA151.1195337E-4148.466541
TGAGTTG151.1195337E-4148.466541
TGCGGTT151.1195337E-4148.466541
TGCTGGT100.0066073076148.466541
ACCCGCA100.0066073076148.466541
TGCAGCT1200.0148.466541
AGCAGTT151.1195337E-4148.466541
TGCACGG100.0066073076148.466541
TGCGTTG450.0148.466541
TGCCGCG359.094947E-12148.466541
TGCAGTT211400.0148.431431
TCCCGCA145650.0148.415571
TTCCAGA332850.0148.243521
TCCCGGA2900.0145.906771
GTCGCAG151.230822E-4144.97659
GATGTGA351.0913936E-11144.97658
GATCGAT151.230822E-4144.97656
ATGGAAA151.230822E-4144.97656
Produced by FastQC (version 0.11.2)