##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AG1W8 l01n01 mis04.3410000000d18d.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	995026
Sequences flagged as poor quality	0
Sequence length	151
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.05741659011925	34.0	34.0	34.0	34.0	34.0
2	33.551033842331755	34.0	34.0	34.0	34.0	34.0
3	33.8837316813832	34.0	34.0	34.0	34.0	34.0
4	33.76365441707051	34.0	34.0	34.0	34.0	34.0
5	33.80179010397718	34.0	34.0	34.0	34.0	34.0
6	36.39648109697636	38.0	38.0	38.0	34.0	38.0
7	37.1297383183957	38.0	38.0	38.0	36.0	38.0
8	37.21851288308044	38.0	38.0	38.0	36.0	38.0
9	37.451588199705334	38.0	38.0	38.0	37.0	38.0
10-14	37.3209514123249	38.0	38.0	38.0	36.8	38.0
15-19	37.24026769149751	38.0	38.0	38.0	36.2	38.0
20-24	37.46490885665299	38.0	38.0	38.0	37.0	38.0
25-29	37.193503084341515	38.0	38.0	38.0	36.2	38.0
30-34	37.01730809044186	38.0	38.0	38.0	36.0	38.0
35-39	37.02203118310476	38.0	38.0	38.0	35.8	38.0
40-44	37.078072130778494	38.0	38.0	38.0	36.2	38.0
45-49	36.85116147718753	38.0	38.0	38.0	35.2	38.0
50-54	37.00435425807969	38.0	38.0	38.0	36.2	38.0
55-59	36.545787145260526	38.0	37.6	38.0	34.4	38.0
60-64	36.568579916504696	38.0	37.4	38.0	34.0	38.0
65-69	36.63171454816256	38.0	37.4	38.0	34.6	38.0
70-74	36.55129313203876	38.0	37.4	38.0	34.2	38.0
75-79	36.29896666016768	38.0	37.2	38.0	33.4	38.0
80-84	36.33242347436148	38.0	37.2	38.0	33.8	38.0
85-89	36.017891391782726	38.0	37.0	38.0	32.2	38.0
90-94	36.23441678910903	38.0	37.0	38.0	33.6	38.0
95-99	35.911876674579354	38.0	36.8	38.0	32.2	38.0
100-104	35.77433152500537	38.0	36.8	38.0	31.0	38.0
105-109	36.03697833021448	38.0	37.0	38.0	32.6	38.0
110-114	35.816586903256805	38.0	37.0	38.0	31.0	38.0
115-119	35.247147913722856	38.0	35.8	38.0	28.2	38.0
120-124	35.353326244741346	38.0	36.6	38.0	28.6	38.0
125-129	35.48634508043006	38.0	37.0	38.0	29.8	38.0
130-134	35.264596101006404	38.0	36.4	38.0	28.2	38.0
135-139	35.212369325022664	38.0	36.2	38.0	28.0	38.0
140-144	35.39293204398679	38.0	36.8	38.0	28.8	38.0
145-149	35.08519234673265	37.8	35.6	38.0	27.6	38.0
150-151	33.09254984291868	36.0	31.5	37.5	23.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.8109543728038631
1101	2	0.3468174940463129
1101	3	0.05494120123847779
1101	4	0.041289510106736316
1101	5	0.1016029615394558
1101	6	1.1777774840669224
1101	7	0.42882602421777705
1101	8	0.3369688427719808
1101	9	0.14621342536756288
1101	10-14	0.21648830022910204
1101	15-19	0.24235745633723837
1101	20-24	0.13808654290670574
1101	25-29	0.26962241905552986
1101	30-34	0.20031877831240053
1101	35-39	0.18112934816449666
1101	40-44	0.22730201246593396
1101	45-49	0.2894730440557254
1101	50-54	0.1497260489924841
1101	55-59	0.2562268205153586
1101	60-64	0.30636891968698166
1101	65-69	0.2780529599746089
1101	70-74	0.2639787484962497
1101	75-79	0.3860553975707859
1101	80-84	0.43351085904029674
1101	85-89	0.5562372354303378
1101	90-94	0.4081246401305947
1101	95-99	0.4668279991786406
1101	100-104	0.6659961540523938
1101	105-109	0.43903376697013385
1101	110-114	0.37253094551528676
1101	115-119	0.584922648020715
1101	120-124	0.3181215937562314
1101	125-129	0.342317108168146
1101	130-134	0.47111467162237375
1101	135-139	0.3525472432906298
1101	140-144	0.41216299180126015
1101	145-149	0.6214352000225674
1101	150-151	0.37790046316408876
1102	1	0.8808012829439775
1102	2	0.33859391743223455
1102	3	0.06445310062548515
1102	4	0.09709365811677628
1102	5	0.11202940471927292
1102	6	1.0756452315802463
1102	7	0.4653438207950842
1102	8	0.09238582073819401
1102	9	0.15766666311455424
1102	10-14	0.21376670922438734
1102	15-19	0.23595149343560706
1102	20-24	0.12765140651176665
1102	25-29	0.275155723655395
1102	30-34	0.1602316120005085
1102	35-39	0.2293416850660961
1102	40-44	0.2519520776284594
1102	45-49	0.23977504547094242
1102	50-54	0.17356905011279622
1102	55-59	0.3993920728271476
1102	60-64	0.45132090577197204
1102	65-69	0.3596498479792132
1102	70-74	0.28070569446294513
1102	75-79	0.30186711189768545
1102	80-84	0.5060085558505136
1102	85-89	0.5120431250104005
1102	90-94	0.44567631463554847
1102	95-99	0.454513752623555
1102	100-104	0.7410757357536113
1102	105-109	0.3801355368589725
1102	110-114	0.3556812993540177
1102	115-119	0.6058108926425234
1102	120-124	0.5454288557373843
1102	125-129	0.5464065194955197
1102	130-134	0.5870469494276662
1102	135-139	0.6552250608838506
1102	140-144	0.5533368246383432
1102	145-149	0.6356615733932216
1102	150-151	0.9152662140917656
1103	1	0.8976141640901503
1103	2	0.3652770457524852
1103	3	0.06703466719034168
1103	4	0.10545742937994618
1103	5	0.10866527421276828
1103	6	1.223629436604078
1103	7	0.5173720793153791
1103	8	0.35367595884892467
1103	9	0.18174051181299689
1103	10-14	0.1667928430769834
1103	15-19	0.2953063217392824
1103	20-24	0.11814940574636523
1103	25-29	0.2856200273318521
1103	30-34	0.2824608854931867
1103	35-39	0.2048872143928122
1103	40-44	0.27724826275754566
1103	45-49	0.274102607938957
1103	50-54	0.2101571475747761
1103	55-59	0.4790077264520676
1103	60-64	0.503996447459599
1103	65-69	0.3732188938142684
1103	70-74	0.3131610859721903
1103	75-79	0.3651498627924994
1103	80-84	0.4807411981658589
1103	85-89	0.5246306594134538
1103	90-94	0.4868214943965157
1103	95-99	0.4981964513181083
1103	100-104	0.6687529563539485
1103	105-109	0.3803004368793452
1103	110-114	0.4889457948224205
1103	115-119	0.8612504085291377
1103	120-124	0.7043595423027185
1103	125-129	0.6048556147155253
1103	130-134	0.7010318670729703
1103	135-139	0.6336222491082921
1103	140-144	0.6960605635842825
1103	145-149	0.7485996706352651
1103	150-151	0.7496789392236991
2116	1	0.7695854296117304
2116	2	0.2615602191333011
2116	3	-0.1177286137210416
2116	4	-0.17444809358320867
2116	5	0.08116768968323385
2116	6	1.1015186420537546
2116	7	0.055881196355400675
2116	8	0.1233673006513456
2116	9	0.06355934988743428
2116	10-14	0.15793556121283103
2116	15-19	0.19745687348674323
2116	20-24	0.12865926017976648
2116	25-29	0.25411814611363326
2116	30-34	0.2357719816827526
2116	35-39	0.13785576868115612
2116	40-44	0.005800689727415431
2116	45-49	0.22396209452582383
2116	50-54	0.17691341310679576
2116	55-59	0.32342756460137423
2116	60-64	0.42328495232589347
2116	65-69	0.3521454017643606
2116	70-74	0.246320044063971
2116	75-79	0.2665784133401914
2116	80-84	0.39552879933746254
2116	85-89	0.5300714413845355
2116	90-94	0.43851578398271585
2116	95-99	0.38226741171035883
2116	100-104	0.606056530653639
2116	105-109	0.3543825208151219
2116	110-114	0.3562398623553591
2116	115-119	0.7517337979375824
2116	120-124	0.5611515708176498
2116	125-129	0.5137189222052996
2116	130-134	0.5017151789835239
2116	135-139	0.6004168758014004
2116	140-144	0.5574748435414065
2116	145-149	0.6714959740929274
2116	150-151	1.4316759508713517
1117	1	0.8952746284848985
1117	2	0.34959545441166284
1117	3	0.0802736932082766
1117	4	0.03721985221741875
1117	5	0.13519367031520346
1117	6	1.1731434114730277
1117	7	0.4193682800945453
1117	8	0.24084700281510152
1117	9	0.1584072077494696
1117	10-14	0.1656886689099224
1117	15-19	0.19258440538654042
1117	20-24	0.11977692404730789
1117	25-29	0.29434752250964635
1117	30-34	0.21515590289408948
1117	35-39	0.20097419254876314
1117	40-44	0.22517716054445458
1117	45-49	0.32762496568037847
1117	50-54	0.1596503408813561
1117	55-59	0.3555774222509598
1117	60-64	0.4613156844542985
1117	65-69	0.3708558252848704
1117	70-74	0.3254401794277513
1117	75-79	0.28797884809613095
1117	80-84	0.4438585495996463
1117	85-89	0.542988330590596
1117	90-94	0.4282755012866275
1117	95-99	-0.1587663379773403
1117	100-104	0.4546417486524774
1117	105-109	0.4960199618889334
1117	110-114	0.5530415187964408
1117	115-119	0.867676594468513
1117	120-124	0.6493723478429061
1117	125-129	0.7107949266994638
1117	130-134	0.5358197926925428
1117	135-139	0.38037151516790146
1117	140-144	0.5209422989325461
1117	145-149	-0.5876287392239732
1117	150-151	0.1196712412646832
2117	1	-30.96411234469518
2117	2	-10.568796829497863
2117	3	-1.74849744245941
2117	4	-0.4116876760004544
2117	5	0.04290088491897137
2117	6	1.1707247366405582
2117	7	-0.02535131634919452
2117	8	0.2605943363555383
2117	9	0.2362086545164317
2117	10-14	0.38239217044100826
2117	15-19	0.3990865725783621
2117	20-24	0.2827743056653702
2117	25-29	0.35844847074852026
2117	30-34	0.4083004760386615
2117	35-39	0.3281429376485363
2117	40-44	0.20321884755166764
2117	45-49	0.43902069638645713
2117	50-54	0.3688694121693956
2117	55-59	0.5446695685844958
2117	60-64	0.6545928039383142
2117	65-69	0.43214726450733565
2117	70-74	0.5146465286341098
2117	75-79	0.5592890745787713
2117	80-84	0.6572702864596565
2117	85-89	0.6447282971581458
2117	90-94	0.5850171690627803
2117	95-99	0.030140006791079088
2117	100-104	0.8148982613227815
2117	105-109	0.7065887572853171
2117	110-114	0.7820916526741613
2117	115-119	1.1830362891385562
2117	120-124	0.9209802118645669
2117	125-129	0.7350872978883842
2117	130-134	0.8159606231782064
2117	135-139	0.7917128071988557
2117	140-144	0.7127895156073478
2117	145-149	0.9743174434118629
2117	150-151	1.307869291531702
1116	1	0.2899310478097519
1116	2	0.24944355797329365
1116	3	-0.0973159143548088
1116	4	-0.2801099444227191
1116	5	0.06768137123791718
1116	6	1.1992809344275273
1116	7	-0.1717582473715069
1116	8	0.27590787474599665
1116	9	0.06888579488587965
1116	10-14	0.20073775186351384
1116	15-19	0.20669445095547445
1116	20-24	0.06371988353403424
1116	25-29	0.06645026798108944
1116	30-34	0.22747674444570265
1116	35-39	0.18971571922132568
1116	40-44	0.10747691796358083
1116	45-49	0.2665063731028994
1116	50-54	0.1771941528018317
1116	55-59	0.3329570953335548
1116	60-64	0.4440588887890158
1116	65-69	0.31331201336440273
1116	70-74	0.25908909461512764
1116	75-79	0.2788600741497689
1116	80-84	0.3432319201135954
1116	85-89	0.3576692347144643
1116	90-94	0.4421361646433084
1116	95-99	-0.45962647989848904
1116	100-104	0.6073865399649065
1116	105-109	0.36024721709762275
1116	110-114	0.5052109173318868
1116	115-119	0.9351313089258682
1116	120-124	0.6853518531592826
1116	125-129	0.4932497545123766
1116	130-134	0.49814766228831786
1116	135-139	0.6834493450892367
1116	140-144	0.39526817347061893
1116	145-149	0.4902682885934837
1116	150-151	0.6251440472679874
2118	1	0.7418364504855433
2118	2	0.3262757387674782
2118	3	0.02378134450310654
2118	4	-0.030376394839777277
2118	5	0.0223001421796738
2118	6	1.170598750068791
2118	7	0.3890784425835818
2118	8	0.17955961187201552
2118	9	0.06380927579175477
2118	10-14	0.21452966507850846
2118	15-19	0.19447611013658417
2118	20-24	0.044174572110826205
2118	25-29	0.16184400268321752
2118	30-34	0.20516928977495041
2118	35-39	0.08821239824510485
2118	40-44	0.06235801038058497
2118	45-49	0.19202676829313248
2118	50-54	0.040035963832941945
2118	55-59	0.20559929133650456
2118	60-64	0.2890622454107685
2118	65-69	0.1906308449035734
2118	70-74	0.22706166537152228
2118	75-79	0.1601150942300933
2118	80-84	0.232304690485023
2118	85-89	0.018497666785954436
2118	90-94	0.3346656019190348
2118	95-99	0.31016790474065914
2118	100-104	0.5977603725734468
2118	105-109	0.26287467961039823
2118	110-114	0.49556223384052345
2118	115-119	0.7926439927974585
2118	120-124	0.4748052287919933
2118	125-129	0.34298339320918103
2118	130-134	0.4049349891104086
2118	135-139	0.5061470887265074
2118	140-144	0.3602468158507932
2118	145-149	0.24673284504413573
2118	150-151	0.9598711212443689
1119	1	0.7463434193798832
1119	2	0.3140273964899052
1119	3	0.029734638721727435
1119	4	-0.04619426224966361
1119	5	0.10534632538049493
1119	6	1.250660283064036
1119	7	0.20889211896663085
1119	8	0.3375717350487548
1119	9	0.13174532485203372
1119	10-14	0.21192051149751023
1119	15-19	0.19417986579498603
1119	20-24	0.025949805945437276
1119	25-29	0.15865963557094176
1119	30-34	0.08240046649254396
1119	35-39	0.16665038419888134
1119	40-44	-0.014083556345916293
1119	45-49	0.1689921845348863
1119	50-54	0.16142297684091744
1119	55-59	0.32566266432289837
1119	60-64	0.27064823125379434
1119	65-69	0.21727417617580613
1119	70-74	0.17205322679146917
1119	75-79	0.2653071570043579
1119	80-84	0.23850422050860232
1119	85-89	0.3506644899576088
1119	90-94	0.3438381884736046
1119	95-99	0.28368524103336057
1119	100-104	0.5483100338766533
1119	105-109	0.3334982404950608
1119	110-114	0.38437996166488375
1119	115-119	0.8125528988160227
1119	120-124	0.6006532974357697
1119	125-129	0.37000839286309883
1119	130-134	0.640680248970213
1119	135-139	0.5378595667504413
1119	140-144	0.37290800253548895
1119	145-149	0.5227909264697956
1119	150-151	0.942266039869466
2119	1	0.8930844819009636
2119	2	0.3699492996524967
2119	3	0.07331244017404259
2119	4	0.06578526350497782
2119	5	0.029994406202135337
2119	6	1.1730196539335864
2119	7	0.46490265936001407
2119	8	0.2776003866067711
2119	9	0.11997709023047065
2119	10-14	0.13339536209399228
2119	15-19	0.09191672260534034
2119	20-24	0.07347681129043337
2119	25-29	0.08641412867262233
2119	30-34	0.03853918795474698
2119	35-39	-0.011991486807765739
2119	40-44	-0.28243903765287826
2119	45-49	0.08779229166352565
2119	50-54	0.008176612942399686
2119	55-59	0.13193744523657358
2119	60-64	0.14502907646751595
2119	65-69	0.13124026975826553
2119	70-74	0.1291223353275157
2119	75-79	0.09536681133651825
2119	80-84	0.22464220260376067
2119	85-89	0.2948473135786358
2119	90-94	0.22229307630468753
2119	95-99	0.27527963613923845
2119	100-104	0.5605019793481176
2119	105-109	0.26075893480268064
2119	110-114	0.32089680787523633
2119	115-119	0.8744257951106036
2119	120-124	0.44876739783081376
2119	125-129	0.38424990428559624
2119	130-134	0.4500827695028349
2119	135-139	0.2207372874511364
2119	140-144	0.16999810851673658
2119	145-149	0.2940156293737459
2119	150-151	0.32137721361536364
1118	1	0.8639201996835197
1118	2	0.33346722661234907
1118	3	0.06769887854459711
1118	4	0.09244922717491022
1118	5	0.11021095703673467
1118	6	1.2309377391612557
1118	7	0.424913350261626
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2104	150-151	-0.05132363932728623
2111	1	0.9487706379552421
2111	2	0.36585543073579174
2111	3	0.08367359901395588
2111	4	0.10306295502925167
2111	5	0.07162396015367278
2111	6	0.9628291333961201
2111	7	0.37737034218641696
2111	8	-0.037381380224829286
2111	9	-6.19535800126414E-4
2111	10-14	-0.05512242056195049
2111	15-19	-0.03775558519402722
2111	20-24	-0.02155859866753218
2111	25-29	0.02648993413883005
2111	30-34	-0.028336160597973503
2111	35-39	-0.18334729012075712
2111	40-44	-0.13490532313064563
2111	45-49	-0.12893795994429524
2111	50-54	-0.29977863787400594
2111	55-59	-0.29973297349246764
2111	60-64	-0.22699709160507098
2111	65-69	-0.34393446506275893
2111	70-74	-0.2662320243374765
2111	75-79	-0.4830742189473156
2111	80-84	-0.20805550444752896
2111	85-89	-0.42371423174949285
2111	90-94	-0.6637571866883221
2111	95-99	-0.30226349305428357
2111	100-104	-0.2837778233998094
2111	105-109	-0.27565860635738915
2111	110-114	-0.5545838933037075
2111	115-119	-1.983437826172782
2111	120-124	-1.1194495867047678
2111	125-129	-0.7489947908448613
2111	130-134	-0.5240272051817172
2111	135-139	-0.34390719818859594
2111	140-144	-0.7681079931971411
2111	145-149	-1.1178358394390955
2111	150-151	-2.197343562518366
2110	1	0.9698883968695213
2110	2	0.34235283916014936
2110	3	0.07274681148784623
2110	4	0.08278900312583914
2110	5	0.08327178179709449
2110	6	1.1933510495894524
2110	7	0.21316385542515803
2110	8	0.04273799280397128
2110	9	-0.0638520927078261
2110	10-14	0.05404115933137632
2110	15-19	-0.13577302879959063
2110	20-24	-0.1299400454249522
2110	25-29	-0.023284414210166915
2110	30-34	0.06991056857200562
2110	35-39	-0.19008748091557948
2110	40-44	-0.16175152565007522
2110	45-49	-0.04543646059573092
2110	50-54	-0.17892338300938349
2110	55-59	-0.28103609894542103
2110	60-64	-0.0996953154221103
2110	65-69	-0.07265424763566841
2110	70-74	-0.12176782947101827
2110	75-79	-0.20584144605786747
2110	80-84	-0.23128914537007716
2110	85-89	-0.21923141974928484
2110	90-94	-0.2574963439735356
2110	95-99	-0.4856579459368078
2110	100-104	0.0973583473423858
2110	105-109	-0.1053692359852505
2110	110-114	-0.39417884951402016
2110	115-119	-6.26526123769068
2110	120-124	-2.753621101668898
2110	125-129	-0.18392018886103756
2110	130-134	-0.45417402760160996
2110	135-139	-0.4474692956095012
2110	140-144	-0.4024639549297717
2110	145-149	-0.345804490058228
2110	150-151	-0.6848646628262856
2109	1	0.9485514107633364
2109	2	0.36349229056418153
2109	3	0.04856662098019626
2109	4	0.06902229160908036
2109	5	0.09351403706050121
2109	6	1.050044480589122
2109	7	0.41414650961151267
2109	8	-0.054399696979110956
2109	9	-0.12061619903331433
2109	10-14	0.009489635966424714
2109	15-19	-0.06430311155367718
2109	20-24	-0.29368724634202437
2109	25-29	-0.02457098039320016
2109	30-34	-0.12413570312240552
2109	35-39	-0.3831182299445075
2109	40-44	-0.11919801722328316
2109	45-49	-0.39539047710768926
2109	50-54	-0.18805248165206478
2109	55-59	-0.5599341305606842
2109	60-64	-0.3836316962949198
2109	65-69	-0.3822128223418275
2109	70-74	-0.461197375157397
2109	75-79	-0.3853175095474626
2109	80-84	-0.3285072253003847
2109	85-89	-0.49418110507003377
2109	90-94	-0.6092367542304515
2109	95-99	-0.24862512765091083
2109	100-104	-0.33983816568438385
2109	105-109	-0.37162982311667037
2109	110-114	-1.0691546029389087
2109	115-119	-4.954688678499082
2109	120-124	-1.3983535635827522
2109	125-129	-0.8134126056333528
2109	130-134	-0.8939073233709678
2109	135-139	-1.1226198348630447
2109	140-144	-0.4933304846174593
2109	145-149	-0.4307899097304215
2109	150-151	-1.5292688447330782
2108	1	0.8806588971348859
2108	2	0.20198445598930448
2108	3	0.09053118701039153
2108	4	0.013800311987537839
2108	5	0.00974009808170706
2108	6	0.8956755277678212
2108	7	-0.11305509155296534
2108	8	-0.4314653704100522
2108	9	-0.37447818778805697
2108	10-14	-0.2171696813267232
2108	15-19	-0.34444624595445816
2108	20-24	-0.43586765562363894
2108	25-29	-0.1977546861899313
2108	30-34	-4.532899652661463
2108	35-39	-0.8849109776112556
2108	40-44	-0.5059598689799927
2108	45-49	-0.2016188927825482
2108	50-54	-0.22572265889326104
2108	55-59	-0.31092415539158935
2108	60-64	-0.6022052756538798
2108	65-69	-0.45966986775096785
2108	70-74	-0.48014805586636555
2108	75-79	-0.6518425402293104
2108	80-84	-0.6272260796543634
2108	85-89	-0.9738732876199023
2108	90-94	-0.8059306064438161
2108	95-99	-0.6063373318921421
2108	100-104	-0.5191321977076768
2108	105-109	-0.6815279308313791
2108	110-114	-0.9057808837277861
2108	115-119	-1.0656849110532391
2108	120-124	-0.9552512717198667
2108	125-129	-0.7451815213510216
2108	130-134	-0.9177282127165185
2108	135-139	-1.372492469506419
2108	140-144	-0.9595957898661922
2108	145-149	-1.0349044886041838
2108	150-151	-1.3287736539347819
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	11.0
17	28.0
18	96.0
19	166.0
20	403.0
21	774.0
22	1645.0
23	2746.0
24	3522.0
25	4287.0
26	5192.0
27	6788.0
28	8290.0
29	11043.0
30	14257.0
31	19059.0
32	26278.0
33	34268.0
34	45984.0
35	72122.0
36	145985.0
37	592080.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.10301331969427377	0.07677116532660162	99.64959005638462	0.17062545859451136
2	51.2862980464832	0.03497396047942466	30.49236904362298	18.186358949414387
3	0.0663299250471847	0.10602738018906038	0.09115339699666139	99.7364892977671
4	0.32521763250407526	26.33056824645788	0.14321233817005788	73.20100178286798
5	69.11960089485099	30.521614510575603	0.16974430818893174	0.1890402863844764
6	31.350537573892538	25.571593104099794	25.45963623061106	17.618233091396608
7	0.6307372872668654	48.055126197707395	26.345844229196018	24.968292285829715
8	69.05950196276278	0.2912486708890019	30.256495810159734	0.3927535561884815
9	51.04087732380862	18.0379206171497	0.6791782325285972	30.242023826513076
10-14	18.682868588358495	40.08415860490078	24.548504260190185	16.684468546550544
15-19	31.03997282483071	22.202093211634672	18.902099040628084	27.855834922906535
20-24	34.94035331740075	23.502963741650973	10.514519218593282	31.042163722354992
25-29	24.904977357375586	45.274213940138246	5.409727987007375	24.411080715478793
30-34	22.521763250407528	16.921849278310315	37.93926992862498	22.617117542657176
35-39	43.28845678404383	19.255054641788256	18.95550468027971	18.5009838938882
40-44	14.002327577369838	32.42490145986135	26.585596758275663	26.987174204493147
45-49	16.902915099705936	25.368161233977805	23.074412125914296	34.65451154040196
50-54	28.98036835218376	25.077716562180285	17.750792441604542	28.191122644031413
55-59	22.08227724702671	42.427494356931376	29.156866252741136	6.333362143300778
60-64	36.75097937139331	20.3586438947324	19.037311587837905	23.853065146036386
65-69	20.018089979558322	33.385961773863194	26.161326437701128	20.434621808877356
70-74	25.104308832131018	13.860783537314603	29.63170811616983	31.40319951438455
75-79	28.64198523455668	16.680287751274843	29.399131278981656	25.27859573518682
80-84	25.394934403724122	19.83037629167479	31.128191625143465	23.646497679457624
85-89	31.051349411975167	21.228872411374176	37.37421936713211	10.345558809518545
90-94	27.298663552510188	34.54142906818515	27.6969446024526	10.462962776852063
95-99	15.534790045687249	31.076454283606658	31.014104154062306	22.374651516643787
100-104	10.039998954801181	38.10593894028899	30.113725671489995	21.74033643341983
105-109	18.926822012691126	25.670163392715367	32.656895397708205	22.74611919688531
110-114	41.6753130068963	26.68509164584644	11.58713440653812	20.052460940719136
115-119	8.643151033239333	40.26401320166508	17.500587924335644	33.592247840759946
120-124	5.588879084566634	20.813466180783216	45.182678643573134	28.414976091077015
125-129	20.51765481505006	29.747282985570227	19.523409438547333	30.21165276083238
130-134	18.137073805106603	30.78970800762995	44.45425546669132	6.618962720572126
135-139	30.384613065387235	1.0567965058199484	47.12099985327017	21.43759057552265
140-144	12.607308753741108	38.33958107627338	17.977680985220488	31.075429184765017
145-149	23.729229185971022	19.863038754766208	34.639054657868236	21.768677401394537
150-151	39.501430113383975	18.74247507100317	28.56437922225148	13.19171559336138
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	0.5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.5
19	0.5
20	0.0
21	0.0
22	0.5
23	1.0
24	0.5
25	0.0
26	0.5
27	1.0
28	1.5
29	6.5
30	11.0
31	29.0
32	55.5
33	132.5
34	540.0
35	4638.5
36	109480.0
37	117681.5
38	13511.0
39	1440.0
40	569.5
41	460.0
42	461.0
43	567.0
44	829.5
45	1651.0
46	9026.5
47	80106.0
48	83043.5
49	21775.5
50	135730.0
51	137120.0
52	15334.5
53	5298.0
54	3270.5
55	1299.5
56	593.0
57	417.0
58	379.0
59	633.0
60	1722.5
61	9415.0
62	109570.0
63	113418.0
64	13089.5
65	1348.5
66	261.0
67	58.5
68	22.5
69	8.0
70	5.0
71	5.0
72	3.0
73	1.5
74	0.5
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.342350853143536
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	995026.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	1.2584643186090634
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.59518769756505	0.9261691773872233
2	9.40339124415934	0.2366766470939084
3	3.8052734425319716	0.14366402549931484
4	1.9584411850711543	0.09858513406025989
5	1.2974160845026599	0.08163759243680395
6	0.8777229249343014	0.06627497895929998
7	0.7199738116475374	0.06342429466039717
8	0.48436954032547613	0.04876494268165485
9	0.4516564975618574	0.05115542278045851
>10	4.102323864796746	1.1246909010557036
>50	1.2330470418446116	1.14311933625822
>100	1.7568905563537602	4.197421422268006
>500	0.11282783389427817	1.0125901386924026
>1k	0.12088696488672664	4.016587265888944
>5k	0.04835478595469065	3.5539033503526114
>10k+	0.03223652396979377	83.23533536992478
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	254344	25.56154311545628	No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	214290	21.53612066418365	No Hit
TGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	210309	21.136030616285403	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	141746	14.245456902633698	No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC	6764	0.679781231847208	No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	6390	0.64219427432047	No Hit
NGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	5668	0.5696333563143073	No Hit
TGCAGTTGGTCGACAGTCCTCGGTCCGCAACGGTGTGGACATGGCAGAGG	5569	0.5596838675572297	No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	5563	0.5590808682386189	No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG	5087	0.5112429222954978	No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG	4067	0.40873303813166695	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG	3942	0.3961705523272759	No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	3758	0.37767857322321224	No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	3624	0.36421158844090507	No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	3615	0.3633070894629889	No Hit
TGCAGTTGGTCGACAGTCCTCGGTACGTGAGAAAACGAGAAAGTGAGCTG	3284	0.3300416270529614	No Hit
TGCAGTTGGTCGACAGTCCTCGGCACGTGAGAAAACGAGAAAGTGAGCTG	3212	0.3228056352296322	No Hit
TGCAGATGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	3013	0.3028061578290417	No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	1870	0.18793478763368998	No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC	1860	0.1869297887693387	No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	1803	0.1812012952425364	No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC	1645	0.1653223131857861	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA	1430	0.1437148376022335	No Hit
TGCAGCTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	1272	0.12783585554548324	No Hit
TTCCATATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	1208	0.12140386281363502	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.003416996138794363
6	0.0	0.0	0.003416996138794363
7	0.0	0.0	0.003416996138794363
8	0.0	0.0	0.003416996138794363
9	0.0	0.0	0.003416996138794363
10-11	0.0	0.0	0.003416996138794363
12-13	0.0	0.0	0.003416996138794363
14-15	0.0	0.0	0.003416996138794363
16-17	0.0	0.0	0.003416996138794363
18-19	0.0	0.0	0.003416996138794363
20-21	0.0	0.0	0.003416996138794363
22-23	0.0	0.0	0.005879243356455007
24-25	0.0	0.0	0.005929493299672572
26-27	0.0	0.0	0.005979743242890136
28-29	0.0	0.0	0.006080243129325264
30-31	0.0	0.0	0.006130493072542828
32-33	0.0	0.0	0.00663299250471847
34-35	0.0	0.0	0.007135491936894112
36-37	0.0	0.0	0.00723599182332924
38-39	0.0	0.0	0.00723599182332924
40-41	0.0	0.0	0.008140490801245393
42-43	0.0	0.0	0.013064985236566682
44-45	0.0	0.0	0.018642728933716303
46-47	0.0	0.0	0.04421995003145646
48-49	0.0	0.0	0.8984187347868298
50-51	0.0	0.0	0.9801753924018066
52-53	0.0	0.0	0.9907781304207126
54-55	0.0	0.0	0.997310623038996
56-57	0.0	0.0	1.0234405935121293
58-59	0.0	0.0	1.0382643267613108
60-61	0.0	0.0	1.0451485689821172
62-63	0.0	0.0	1.052183561032576
64-65	0.0	0.0	1.0538920591019731
66-67	0.0	0.0	1.0544950584205839
68-69	0.0	0.0	1.056103056603546
70-71	0.0	0.0	1.0837405253732064
72-73	0.0	0.0	1.0840922749757294
74-75	0.0	0.0	1.0843937746350347
76-77	0.0	0.0	1.0861022727044318
78-79	0.0	0.0	1.0925845153794977
80-81	0.0	0.0	1.0961522613479446
82-83	0.0	0.0	1.0961522613479446
84-85	0.0	0.0	1.0961522613479446
86-87	0.0	0.0	1.0961522613479446
88-89	0.0	0.0	1.0962025112911622
90-91	0.0	0.0	1.0962527612343798
92-93	0.0	0.0	1.0963532611208149
94-95	0.0	0.0	1.09645376100725
96-97	0.0	0.0	1.0965542608936851
98-99	0.0	0.0	1.0966547607801203
100-101	0.0	0.0	1.096805510609773
102-103	0.0	0.0	1.104192252262755
104-105	0.0	0.0	1.110875494710691
106-107	0.0	0.0	1.111679493802172
108-109	0.0	0.0	1.111779993688607
110-111	0.0	0.0	1.1121317432911302
112-113	0.0	0.0	1.1121317432911302
114-115	0.0	0.0	1.1121317432911302
116-117	0.0	0.0	1.1126844926665234
118-119	0.0	0.0	1.112835242496176
120-121	0.0	0.0	1.114091491076615
122-123	0.0	0.0	1.2039383895496198
124-125	0.0	0.0	1.2908205413727882
126-127	0.0	0.0	1.3053930249058818
128-129	0.0	0.0	1.3054432748490994
130-131	0.0	0.0	1.3058955243380574
132-133	0.0	0.0	1.3059960242244926
134-135	0.0	0.0	1.3059960242244926
136-137	0.0	0.0	1.3059960242244926
138-139	0.0	0.0	1.3059960242244926
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTAGA	25	3.2248863E-8	148.45293	1
TGCATTG	30	5.529728E-10	148.45293	1
TCCCGCG	15	1.1199207E-4	148.45293	1
TCCCGCA	16440	0.0	148.45293	1
TGCAGGT	560	0.0	148.45293	1
TGCGTTG	25	3.2248863E-8	148.45293	1
TGAGTTG	40	0.0	148.45291	1
TTCCGCA	40	0.0	148.45291	1
TGCGGTT	20	1.8950705E-6	148.45291	1
TCTCGCA	10	0.0066090547	148.45291	1
TCCCGGA	395	0.0	148.45291	1
TACAGTT	10	0.0066090547	148.45291	1
TGCAGAC	20	1.8950705E-6	148.45291	1
TGCCGAG	20	1.8950705E-6	148.45291	1
TGCAGTT	23845	0.0	148.42178	1
TTCCAGA	28600	0.0	148.14148	1
GATCATG	105	0.0	144.9766	8
CAGCTGG	105	0.0	144.9766	3
TCGCCCC	15	1.2307949E-4	144.97658	145
CTTCGCT	10	0.0070942813	144.97658	145
>>END_MODULE