FastQCFastQC Report
Wed 17 Jun 2015
000000000-AG1W8 l01n01 mis04.3410000000d18d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG1W8 l01n01 mis04.3410000000d18d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences995026
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC25434425.56154311545628No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG21429021.53612066418365No Hit
TGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG21030921.136030616285403No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG14174614.245456902633698No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC67640.679781231847208No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC63900.64219427432047No Hit
NGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG56680.5696333563143073No Hit
TGCAGTTGGTCGACAGTCCTCGGTCCGCAACGGTGTGGACATGGCAGAGG55690.5596838675572297No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG55630.5590808682386189No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG50870.5112429222954978No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG40670.40873303813166695No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG39420.3961705523272759No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG37580.37767857322321224No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG36240.36421158844090507No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG36150.3633070894629889No Hit
TGCAGTTGGTCGACAGTCCTCGGTACGTGAGAAAACGAGAAAGTGAGCTG32840.3300416270529614No Hit
TGCAGTTGGTCGACAGTCCTCGGCACGTGAGAAAACGAGAAAGTGAGCTG32120.3228056352296322No Hit
TGCAGATGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG30130.3028061578290417No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC18700.18793478763368998No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC18600.1869297887693387No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG18030.1812012952425364No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC16450.1653223131857861No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA14300.1437148376022335No Hit
TGCAGCTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG12720.12783585554548324No Hit
TTCCATATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC12080.12140386281363502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTAGA253.2248863E-8148.452931
TGCATTG305.529728E-10148.452931
TCCCGCG151.1199207E-4148.452931
TCCCGCA164400.0148.452931
TGCAGGT5600.0148.452931
TGCGTTG253.2248863E-8148.452931
TGAGTTG400.0148.452911
TTCCGCA400.0148.452911
TGCGGTT201.8950705E-6148.452911
TCTCGCA100.0066090547148.452911
TCCCGGA3950.0148.452911
TACAGTT100.0066090547148.452911
TGCAGAC201.8950705E-6148.452911
TGCCGAG201.8950705E-6148.452911
TGCAGTT238450.0148.421781
TTCCAGA286000.0148.141481
GATCATG1050.0144.97668
CAGCTGG1050.0144.97663
TCGCCCC151.2307949E-4144.97658145
CTTCGCT100.0070942813144.97658145
Produced by FastQC (version 0.11.2)