##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AG1W8 l01n01 mis03.3410000000d170.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	834679
Sequences flagged as poor quality	0
Sequence length	151
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.045470174761796	34.0	34.0	34.0	34.0	34.0
2	33.54727266410201	34.0	34.0	34.0	34.0	34.0
3	33.886967325163326	34.0	34.0	34.0	34.0	34.0
4	33.773039695499705	34.0	34.0	34.0	34.0	34.0
5	33.81125917867827	34.0	34.0	34.0	34.0	34.0
6	36.439016675871805	38.0	38.0	38.0	34.0	38.0
7	37.144377658956316	38.0	38.0	38.0	36.0	38.0
8	37.25945183717333	38.0	38.0	38.0	37.0	38.0
9	37.465936006536644	38.0	38.0	38.0	37.0	38.0
10-14	37.318096657517444	38.0	38.0	38.0	36.6	38.0
15-19	37.288440466334954	38.0	38.0	38.0	36.2	38.0
20-24	37.48848191939656	38.0	38.0	38.0	37.2	38.0
25-29	37.2189416530187	38.0	38.0	38.0	36.4	38.0
30-34	37.07829548844526	38.0	38.0	38.0	36.0	38.0
35-39	37.108034106524784	38.0	38.0	38.0	36.2	38.0
40-44	37.105443889207706	38.0	38.0	38.0	36.2	38.0
45-49	36.904769618020815	38.0	38.0	38.0	35.2	38.0
50-54	37.06484792357302	38.0	38.0	38.0	36.2	38.0
55-59	36.692330824185106	38.0	37.8	38.0	34.8	38.0
60-64	36.70581337256598	38.0	37.8	38.0	34.6	38.0
65-69	36.74728009210727	38.0	37.4	38.0	34.8	38.0
70-74	36.66607162753586	38.0	37.8	38.0	34.6	38.0
75-79	36.42111350591066	38.0	37.2	38.0	33.6	38.0
80-84	36.43386595325868	38.0	37.2	38.0	34.0	38.0
85-89	36.15276795031384	38.0	37.0	38.0	32.8	38.0
90-94	36.37815040272967	38.0	37.0	38.0	34.0	38.0
95-99	36.069128850731836	38.0	36.8	38.0	32.8	38.0
100-104	35.947013642370294	38.0	36.8	38.0	31.8	38.0
105-109	36.2137669690983	38.0	37.0	38.0	33.0	38.0
110-114	36.0417688716261	38.0	37.0	38.0	32.4	38.0
115-119	35.43982489076639	38.0	36.4	38.0	29.0	38.0
120-124	35.58260720588394	38.0	36.6	38.0	29.8	38.0
125-129	35.83384175233832	38.0	37.0	38.0	31.6	38.0
130-134	35.69557590403017	38.0	37.0	38.0	31.0	38.0
135-139	35.56102262067214	38.0	36.8	38.0	29.8	38.0
140-144	35.663320869459994	38.0	37.0	38.0	30.6	38.0
145-149	35.37087574983916	38.0	36.4	38.0	29.0	38.0
150-151	33.502354797473046	36.5	32.5	37.5	24.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7964009153636056
1101	2	0.3711846815190256
1101	3	0.04550271788160387
1101	4	0.044110012249049646
1101	5	0.11286881968030826
1101	6	1.21220873532409
1101	7	0.3994348516307298
1101	8	0.23784395507298228
1101	9	0.15808293396921158
1101	10-14	0.24117956482313474
1101	15-19	0.2611393851734434
1101	20-24	0.16825984111406456
1101	25-29	0.27566351241669196
1101	30-34	0.2245213725959303
1101	35-39	0.18885867735574635
1101	40-44	0.2369361596696038
1101	45-49	0.3435885680043853
1101	50-54	0.1932259644050447
1101	55-59	0.3257034889621906
1101	60-64	0.35671626124953093
1101	65-69	0.32087295533457194
1101	70-74	0.3152672337430005
1101	75-79	0.4399565087180761
1101	80-84	0.457402298418387
1101	85-89	0.5118860683904884
1101	90-94	0.4177030365903178
1101	95-99	0.5089498929732343
1101	100-104	0.6076205430814952
1101	105-109	0.36874795824770956
1101	110-114	0.4399255085751932
1101	115-119	0.6189043602457502
1101	120-124	0.4553333373250368
1101	125-129	0.3671387005238742
1101	130-134	0.5065529384782224
1101	135-139	0.5532104037235896
1101	140-144	0.5813547209515164
1101	145-149	0.7218327241172986
1101	150-151	0.2993224846381253
1102	1	0.899100684730044
1102	2	0.36096758951181585
1102	3	0.03762713438873533
1102	4	0.041570028227582156
1102	5	0.04958528108812743
1102	6	1.108213850465006
1102	7	0.44780509135899393
1102	8	0.1671081636581775
1102	9	0.1479437247559261
1102	10-14	0.21382461524596152
1102	15-19	0.2597789486278259
1102	20-24	0.11165048110964193
1102	25-29	0.23393127346223253
1102	30-34	0.15230141148708043
1102	35-39	0.268117610009746
1102	40-44	0.30434334096567284
1102	45-49	0.2759723593388017
1102	50-54	0.16253361149047407
1102	55-59	0.3469687577583258
1102	60-64	0.4619910618947003
1102	65-69	0.34824762684504407
1102	70-74	0.24178720439876855
1102	75-79	0.2869985955749783
1102	80-84	0.5373743611065009
1102	85-89	0.45281523524558054
1102	90-94	0.4005784944657762
1102	95-99	0.4629276938646143
1102	100-104	0.7234911722237314
1102	105-109	0.3657753478676966
1102	110-114	0.34132174608253507
1102	115-119	0.6139755099465205
1102	120-124	0.5509918740329596
1102	125-129	0.5073244635491463
1102	130-134	0.5505182781225813
1102	135-139	0.7518938076539072
1102	140-144	0.5076826225880637
1102	145-149	0.6583787227003342
1102	150-151	0.9480798772226819
1103	1	0.8967893061720673
1103	2	0.34851445636511613
1103	3	0.05106409784836785
1103	4	0.08919903868524415
1103	5	0.09624955667352708
1103	6	1.2607689864743605
1103	7	0.49989247209551024
1103	8	0.1945155175614488
1103	9	0.15864754948967175
1103	10-14	0.12898871980262783
1103	15-19	0.19961818162209255
1103	20-24	0.08065966020494386
1103	25-29	0.2061139487500796
1103	30-34	0.2389727938973607
1103	35-39	0.10712270434122217
1103	40-44	0.22041630547472124
1103	45-49	0.1536523629254205
1103	50-54	0.08648826064911219
1103	55-59	0.2601211991519321
1103	60-64	0.3851110742486199
1103	65-69	0.24037441060827547
1103	70-74	0.17678541108781332
1103	75-79	0.1902347746271147
1103	80-84	0.321119325721817
1103	85-89	0.3825121324855303
1103	90-94	0.27019623695296247
1103	95-99	0.3070969425669503
1103	100-104	0.49541630813344995
1103	105-109	0.2121676379589843
1103	110-114	0.2718478005659861
1103	115-119	0.7145114276401898
1103	120-124	0.533869656570765
1103	125-129	0.36750914136754886
1103	130-134	0.46397425399922554
1103	135-139	0.4266213240178729
1103	140-144	0.5074498242591545
1103	145-149	0.5656355713216428
1103	150-151	0.64084004845067
2116	1	0.7424457256059469
2116	2	0.32454544969461097
2116	3	-0.053719961587987086
2116	4	-0.14839113086937772
2116	5	0.04290997194368629
2116	6	1.0860084609756697
2116	7	-0.053240045488614385
2116	8	0.07094866472090189
2116	9	0.08492335463679268
2116	10-14	0.121746551562957
2116	15-19	0.15021117301066766
2116	20-24	0.08961329333171619
2116	25-29	0.18622592668281612
2116	30-34	0.287438610821809
2116	35-39	0.06265840300733316
2116	40-44	0.0036485510733470505
2116	45-49	0.18300329136972238
2116	50-54	0.14411759677815894
2116	55-59	0.2554702928030679
2116	60-64	0.4005205593748187
2116	65-69	0.3116365584438512
2116	70-74	0.15779123922995808
2116	75-79	0.23968216029557965
2116	80-84	0.3759208169369117
2116	85-89	0.4778668640009158
2116	90-94	0.4244702976785675
2116	95-99	0.40524618926952627
2116	100-104	0.5806429482026658
2116	105-109	0.29284489469032593
2116	110-114	0.2927833046428603
2116	115-119	0.691240894310674
2116	120-124	0.5365404703840255
2116	125-129	0.4497175756953382
2116	130-134	0.45726167190301226
2116	135-139	0.5703114553925346
2116	140-144	0.5979070757754741
2116	145-149	0.7246676578164255
2116	150-151	1.3839132482412353
1117	1	0.8812435418728981
1117	2	0.36142546496969175
1117	3	0.06326816002976443
1117	4	0.01638687804443606
1117	5	0.11094059244344123
1117	6	1.1631589054100644
1117	7	0.35027761883151953
1117	8	0.2032803248303381
1117	9	0.1122294390416414
1117	10-14	0.17087589729724328
1117	15-19	0.13972400357287995
1117	20-24	0.06989223935138966
1117	25-29	0.20737449690545873
1117	30-34	0.14177027595594893
1117	35-39	0.10309929499142356
1117	40-44	0.17513088675321598
1117	45-49	0.21800532637175962
1117	50-54	0.10172775068460993
1117	55-59	0.2858881716777333
1117	60-64	0.32911560401924334
1117	65-69	0.3269106305080527
1117	70-74	0.24096302857459762
1117	75-79	0.1734454820218616
1117	80-84	0.3270263757585141
1117	85-89	0.3833097407400743
1117	90-94	0.22823417212145358
1117	95-99	-0.32544226950535204
1117	100-104	0.3860369597695197
1117	105-109	0.30753358962594035
1117	110-114	0.39837302813381825
1117	115-119	0.7331147040857147
1117	120-124	0.5153691634102486
1117	125-129	0.4493574305821042
1117	130-134	0.4611409887452851
1117	135-139	0.3014359321960285
1117	140-144	0.47379983870527553
1117	145-149	-0.7196432553216425
1117	150-151	-0.06222232057952226
2117	1	-30.969031183401825
2117	2	-10.551489037701405
2117	3	-1.7330738515759379
2117	4	-0.3997160352694351
2117	5	0.02760540160317504
2117	6	1.2428310925873234
2117	7	-0.013051665136707413
2117	8	0.22995040930975108
2117	9	0.22654691669583116
2117	10-14	0.4089973262401756
2117	15-19	0.37418762987220333
2117	20-24	0.27649326172321054
2117	25-29	0.39239946179857554
2117	30-34	0.3904772653830122
2117	35-39	0.2741569222863802
2117	40-44	0.19976848715146645
2117	45-49	0.39350963950284523
2117	50-54	0.3209302858100642
2117	55-59	0.44330315358561734
2117	60-64	0.558040701909853
2117	65-69	0.40259214980011393
2117	70-74	0.4094047288621354
2117	75-79	0.5489967900782489
2117	80-84	0.49784884267631924
2117	85-89	0.5623761949079551
2117	90-94	0.43666855383170144
2117	95-99	-0.08935703965744324
2117	100-104	0.7009627709427875
2117	105-109	0.6000835008371013
2117	110-114	0.668866902075969
2117	115-119	0.9813129223650705
2117	120-124	0.7756549222761251
2117	125-129	0.6147767813462863
2117	130-134	0.8212866915887673
2117	135-139	0.7837680692949007
2117	140-144	0.7948316466539183
2117	145-149	1.0037358841325528
2117	150-151	1.390096869696066
1116	1	0.29126223827351794
1116	2	0.23216503459709514
1116	3	-0.09834020715125291
1116	4	-0.3593451159435972
1116	5	0.05313820395009117
1116	6	1.197657968423087
1116	7	-0.20375103511430837
1116	8	0.1428706682304295
1116	9	0.048655826189751394
1116	10-14	0.20079503558126532
1116	15-19	0.17688819212136053
1116	20-24	0.0705191365116633
1116	25-29	0.06777622248701931
1116	30-34	0.2060166818611293
1116	35-39	0.13274756516265285
1116	40-44	0.127089024844814
1116	45-49	0.20649104793840678
1116	50-54	0.13650166898905525
1116	55-59	0.32053038307100934
1116	60-64	0.4541909703629514
1116	65-69	0.2812113227169277
1116	70-74	0.33979027799341566
1116	75-79	0.27348765076966686
1116	80-84	0.3681397780527007
1116	85-89	0.30340022369142616
1116	90-94	0.3864678259892784
1116	95-99	-0.5367550654016711
1116	100-104	0.46014422180862624
1116	105-109	0.3353954737585241
1116	110-114	0.44813726452844094
1116	115-119	0.8435169063951591
1116	120-124	0.6315362192602194
1116	125-129	0.3496063258736015
1116	130-134	0.4614515552887042
1116	135-139	0.6044199020044232
1116	140-144	0.41167656207655057
1116	145-149	0.45838708710765275
1116	150-151	0.4968417715832487
2118	1	0.8918540921175975
2118	2	0.3661382318775779
2118	3	0.06438368858431431
2118	4	0.0363981450069204
2118	5	0.08978897982841261
2118	6	1.244784822401833
2118	7	0.5251053881714896
2118	8	0.21452991534822274
2118	9	0.02361874982119616
2118	10-14	0.1848511496871268
2118	15-19	0.12669527530025704
2118	20-24	0.030190094161454795
2118	25-29	0.1815753863502536
2118	30-34	0.15783463165445255
2118	35-39	0.03289048392895921
2118	40-44	0.06297249410701511
2118	45-49	0.22040166535347083
2118	50-54	0.040601252972741975
2118	55-59	0.12353992823192783
2118	60-64	0.2075376336607846
2118	65-69	0.13583386626055471
2118	70-74	0.2096319387069201
2118	75-79	0.22168222720982556
2118	80-84	0.22726189001841846
2118	85-89	0.03993317602204627
2118	90-94	0.25808244038435646
2118	95-99	0.30507109698361745
2118	100-104	0.5313996368395095
2118	105-109	0.1441000944851254
2118	110-114	0.35842395281888884
2118	115-119	0.6987062940675557
2118	120-124	0.46822213699491755
2118	125-129	0.29844341686321485
2118	130-134	0.324175921292408
2118	135-139	0.34348999376228306
2118	140-144	0.3464399808576104
2118	145-149	0.17569534916329843
2118	150-151	0.9664519748630696
1119	1	0.7744411024433404
1119	2	0.2834985438224251
1119	3	0.022934219272094936
1119	4	-0.04463472746869712
1119	5	0.09487447044511299
1119	6	1.2559559770206903
1119	7	0.1681877015721156
1119	8	0.23281297724435035
1119	9	0.07447075933380631
1119	10-14	0.18309850824866913
1119	15-19	0.1083092166496229
1119	20-24	-0.03814409368493443
1119	25-29	0.1660631399161332
1119	30-34	0.13077979674513074
1119	35-39	0.06609276434083
1119	40-44	-0.08667521064609929
1119	45-49	0.09941461795054352
1119	50-54	0.07184431910626188
1119	55-59	0.21386555770596516
1119	60-64	0.19563528070840874
1119	65-69	0.09011179031937644
1119	70-74	0.013248370351462313
1119	75-79	0.15969382293122436
1119	80-84	0.11308040799898578
1119	85-89	0.24044878050624163
1119	90-94	0.21614554689723775
1119	95-99	0.23183125937834603
1119	100-104	0.4552684001873928
1119	105-109	0.19984144973040685
1119	110-114	0.24868982845062249
1119	115-119	0.6930747013338561
1119	120-124	0.47510322229685187
1119	125-129	0.20871189120774858
1119	130-134	0.4760568638306566
1119	135-139	0.3805272790839851
1119	140-144	0.3386307247226057
1119	145-149	0.49099837438778593
1119	150-151	0.9773717412603062
2119	1	0.8535204787724808
2119	2	0.34651427553019687
2119	3	0.07317727607095037
2119	4	0.02125072488941271
2119	5	0.057947987564041625
2119	6	1.3068281292679629
2119	7	0.4868886493104796
2119	8	0.16162229732325528
2119	9	0.06233829245238098
2119	10-14	0.1505514104633221
2119	15-19	0.10454707358463367
2119	20-24	0.07268614356038938
2119	25-29	0.15331927539740775
2119	30-34	0.07432781275876721
2119	35-39	0.1014734051579893
2119	40-44	-0.17421599278234368
2119	45-49	0.15677787457827463
2119	50-54	0.1198285178419809
2119	55-59	0.22679804796496938
2119	60-64	0.20848529093609613
2119	65-69	0.15617319184607936
2119	70-74	0.1197798092781639
2119	75-79	0.01532985551001076
2119	80-84	0.1664019373038883
2119	85-89	0.2544463085747637
2119	90-94	0.1782600896002009
2119	95-99	0.21445855294263794
2119	100-104	0.5521852768612305
2119	105-109	0.2104363472456754
2119	110-114	0.342691916792333
2119	115-119	0.8789851858052558
2119	120-124	0.45338492123732266
2119	125-129	0.4170245119284317
2119	130-134	0.4302438984142327
2119	135-139	0.22301309716942797
2119	140-144	0.1861607482359915
2119	145-149	0.15003847803932757
2119	150-151	0.2853872239639941
1118	1	0.8443257383142324
1118	2	0.33524859746155045
1118	3	0.0667057698915201
1118	4	0.0850214168802097
1118	5	0.11915399926365922
1118	6	1.268708412662356
1118	7	0.44212913676663135
1118	8	0.3190817061115965
1118	9	0.05910879807270675
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2111	1	0.9200050677033929
2111	2	0.3149747892086694
2111	3	0.07158886231598416
2111	4	0.07137929941616505
2111	5	2.6104210871835676E-4
2111	6	0.9801666103488031
2111	7	0.2685705328140884
2111	8	-0.08966523535659832
2111	9	-0.19089990288080116
2111	10-14	-0.17615896439279055
2111	15-19	-0.06756095285000896
2111	20-24	-0.04461767602058586
2111	25-29	0.04205619451818876
2111	30-34	-0.07635843441414636
2111	35-39	-0.1664630392260733
2111	40-44	-0.14070844632750124
2111	45-49	-0.186807102578598
2111	50-54	-0.2622106098054786
2111	55-59	-0.2091699311389874
2111	60-64	-0.2808629232006652
2111	65-69	-0.44431584416670233
2111	70-74	-0.22230588641846083
2111	75-79	-0.5273723782787556
2111	80-84	-0.13767932084373768
2111	85-89	-0.391178701851814
2111	90-94	-0.6802633648243557
2111	95-99	-0.23030206488238747
2111	100-104	-0.3656492850201758
2111	105-109	-0.3457062739587542
2111	110-114	-0.4387811969514388
2111	115-119	-1.9771543372178044
2111	120-124	-1.0920389457220026
2111	125-129	-0.7127652168853658
2111	130-134	-0.517190449930979
2111	135-139	-0.4334889130736457
2111	140-144	-0.8045442310181059
2111	145-149	-1.0633182584794056
2111	150-151	-2.1452635609483437
2110	1	0.9611499920290214
2110	2	0.29206755082608993
2110	3	0.09060816058010346
2110	4	0.11475321563967356
2110	5	0.07325398239713365
2110	6	1.2508723041044618
2110	7	0.27457318259902763
2110	8	-0.09239472482173028
2110	9	-0.026193616029665634
2110	10-14	-0.015988803034097998
2110	15-19	-0.06191103468389514
2110	20-24	-0.0604560211669849
2110	25-29	-0.055624755278508076
2110	30-34	0.0152943914044954
2110	35-39	-0.12587319444283906
2110	40-44	-0.09809309062453764
2110	45-49	-0.01999829616566018
2110	50-54	-0.10304159986468164
2110	55-59	-0.2272342597279433
2110	60-64	0.03473474551994826
2110	65-69	-0.05310811345236033
2110	70-74	-0.039908905501057745
2110	75-79	-0.16452629358800408
2110	80-84	-0.11323886423053864
2110	85-89	-0.1763134943250435
2110	90-94	-0.13996377737343835
2110	95-99	-0.344762185853547
2110	100-104	0.11990946522841028
2110	105-109	-0.04126739580783578
2110	110-114	-0.4103253511125047
2110	115-119	-6.366673738433608
2110	120-124	-2.7293752437512993
2110	125-129	-0.18908367243036395
2110	130-134	-0.3691493645896955
2110	135-139	-0.42762125306973076
2110	140-144	-0.3878617211224409
2110	145-149	-0.27926479080487354
2110	150-151	-0.6776766390552638
2109	1	0.9537155977140586
2109	2	0.37117329939563604
2109	3	0.06676727179067399
2109	4	0.042002356986088785
2109	5	0.1098943568478461
2109	6	1.2176236824070301
2109	7	0.45986313345995455
2109	8	0.17684774453509533
2109	9	-4.869218506868833E-4
2109	10-14	0.07646983840621147
2109	15-19	0.0117660888123865
2109	20-24	-0.16138501701169616
2109	25-29	0.013414444082734178
2109	30-34	-0.01502648236441928
2109	35-39	-0.23344686985944918
2109	40-44	-0.06181514289667689
2109	45-49	-0.26156831886711984
2109	50-54	-0.07450077043661452
2109	55-59	-0.33818758804179794
2109	60-64	-0.1190467629414087
2109	65-69	-0.14551308311737898
2109	70-74	-0.2409423621719995
2109	75-79	-0.18762692911492707
2109	80-84	-0.244119138241075
2109	85-89	-0.3557634250069057
2109	90-94	-0.431395675856038
2109	95-99	-0.06308371961461035
2109	100-104	-0.11048931795848205
2109	105-109	-0.24953789396914772
2109	110-114	-0.7310990591494289
2109	115-119	-4.720331820777119
2109	120-124	-1.1613346049244342
2109	125-129	-0.5494987847333377
2109	130-134	-0.6616735500778361
2109	135-139	-0.804759731939555
2109	140-144	-0.35228184784851635
2109	145-149	-0.14285309984050087
2109	150-151	-1.215801806186903
2108	1	0.9185343239826409
2108	2	0.1566947105724239
2108	3	0.07843520276945526
2108	4	-0.010704122903163693
2108	5	-0.0234802114628323
2108	6	0.8772897404317987
2108	7	-0.07501756795723225
2108	8	-0.310330290376271
2108	9	-0.32241172969909826
2108	10-14	-0.16196518174439944
2108	15-19	-0.3811768004031961
2108	20-24	-0.4529671110806035
2108	25-29	-0.20364448649323208
2108	30-34	-4.339096027028027
2108	35-39	-0.7387047997545366
2108	40-44	-0.4510865515175837
2108	45-49	-0.3616511296067486
2108	50-54	-0.2909450204064541
2108	55-59	-0.3005606780274235
2108	60-64	-0.43083741739162207
2108	65-69	-0.4469858874570747
2108	70-74	-0.445074802103548
2108	75-79	-0.7368399408290642
2108	80-84	-0.6215891781333625
2108	85-89	-0.8414475132811106
2108	90-94	-0.8540454150533208
2108	95-99	-0.8296396720710248
2108	100-104	-0.5468644567431369
2108	105-109	-0.4681762809386214
2108	110-114	-0.7586856270429863
2108	115-119	-0.8273534762967074
2108	120-124	-1.047349609003625
2108	125-129	-0.6370566040096293
2108	130-134	-0.9465220882126957
2108	135-139	-1.2132878068249937
2108	140-144	-1.1563597349273778
2108	145-149	-1.1422218161922473
2108	150-151	-1.2985843288269479
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	3.0
18	16.0
19	48.0
20	130.0
21	427.0
22	960.0
23	1710.0
24	2223.0
25	2913.0
26	3688.0
27	4986.0
28	6616.0
29	8232.0
30	10999.0
31	14812.0
32	19353.0
33	26573.0
34	37254.0
35	58811.0
36	118838.0
37	516086.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.108593487335668	0.08626126734121424	99.62808264393853	0.17706260138459765
2	54.810052726856675	0.042411513887374665	29.153003729577478	15.994532029678476
3	0.06757088653242743	0.1018355559442612	0.09045393498578495	99.74013962253753
4	0.3256341659488259	26.50491985541747	0.1535919796712269	73.01585399896247
5	70.41940674199303	29.228721460585444	0.17755328695222952	0.17431851046929417
6	30.04280687545751	28.751651832620684	25.648662539730843	15.556878752190961
7	0.6283852834442941	44.576178387140445	26.513426119502228	28.282010209913032
8	70.42168306618473	0.28202458669740105	28.936872737902835	0.3594196092150395
9	54.552588480122296	15.888024018814416	0.6479137488783113	28.911473752184975
10-14	17.57461251570963	42.72924082192076	23.21218097016937	16.483965692200236
15-19	31.449119961086836	20.683328561039634	19.81743879982604	28.05011267804749
20-24	34.321313942246064	23.635050121064506	10.67655949173275	31.36707644495668
25-29	23.373320761634115	45.21393254173161	5.903562926586148	25.50918377004813
30-34	22.536184569157726	16.920540710860102	37.29764376484852	23.24563095513365
35-39	42.36107533554816	20.060118919968037	20.04253132042378	17.53627442406003
40-44	13.808565927739885	31.602807785987185	28.364101648657748	26.224524637615175
45-49	16.776173834492063	27.61753919770355	21.003930852459447	34.60235611534494
50-54	30.322195718354	25.860480496094908	17.120378013583665	26.696945771967428
55-59	22.015265748868725	42.44798299705636	29.48424484143006	6.0525064126448616
60-64	36.432616610697046	20.20839148942288	20.34058602169217	23.018405878187902
65-69	19.65946190092239	34.44747022508054	25.875743848832904	20.017324025164164
70-74	25.446836448502957	13.977061840539895	29.460163727612652	31.115937983344494
75-79	28.877640386304197	17.601976328624538	28.065016611176276	25.455366673894996
80-84	26.89589650632159	20.17273706418875	31.83470531785273	21.096661111636926
85-89	30.772524527393163	20.88091350087878	36.65133542355804	11.695226548170016
90-94	27.60575023452129	34.88639345185395	27.907447054496398	9.600409259128359
95-99	16.39137920086644	31.347044792069767	29.82607685110084	22.43549915596295
100-104	9.363407968811963	38.96086998714476	30.655856922241963	21.019865121801313
105-109	19.559471365638768	25.90739673575111	32.13642609913512	22.396705799475008
110-114	41.86248845364505	27.619336295749626	9.820925170035427	20.697250080569894
115-119	8.970801949012735	40.233574823375214	17.18550484677343	33.61011838083862
120-124	6.0443595681693205	20.176930292962926	45.324801510520814	28.453908628346948
125-129	20.29321451719763	28.904405166537078	19.503066448299286	31.29931386796601
130-134	17.36545426445376	29.67924196008286	46.81308622835844	6.142217547104935
135-139	31.118765417603655	0.8894197649635369	47.26974082251979	20.722073994913014
140-144	12.26078528392352	39.338404344664234	17.34582995379062	31.054980417621625
145-149	25.27086460783127	18.342596375373045	33.88799766137641	22.49854135541927
150-151	40.786278317772464	16.183107517979966	28.39337038550149	14.63724377874608
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.5
26	5.5
27	9.0
28	5.5
29	5.0
30	5.5
31	10.0
32	34.5
33	100.5
34	438.0
35	4220.5
36	101803.0
37	109907.5
38	12943.0
39	1235.0
40	519.5
41	437.0
42	342.0
43	383.5
44	532.0
45	1081.5
46	6208.0
47	57760.0
48	62324.0
49	19538.5
50	109880.0
51	111049.5
52	12156.0
53	1925.0
54	634.5
55	262.0
56	172.5
57	181.0
58	274.0
59	553.0
60	1479.5
61	8372.5
62	96044.5
63	99066.0
64	11260.0
65	1165.5
66	249.5
67	61.5
68	19.0
69	6.5
70	4.5
71	6.0
72	4.5
73	2.0
74	0.5
75	0.5
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.3617462521520247
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	834679.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	1.2626798609076628
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.61277673788298	0.9294937069241692
2	8.78402193969474	0.22182815202047226
3	3.657899150062321	0.1385626677004485
4	1.8433191642479894	0.09310087943684323
5	1.3162685567832861	0.0831012899098125
6	0.9614079532154367	0.07283702763849531
7	0.751518937359129	0.06642494791058698
8	0.5516398894568955	0.055723566311244116
9	0.4850330320811385	0.055119729733545064
>10	4.517344802155041	1.2561914257129068
>50	1.483589444814953	1.4094169651756927
>100	1.6833034085400427	3.982200340730708
>500	0.1331426424833932	1.0981612121097137
>1k	0.1331426424833932	4.325638401598262
>5k	0.047550943744069006	3.1058328693281174
>10k+	0.03804075499525521	83.10636681775898
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	202660	24.279992667839974	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	202357	24.24369128730925	No Hit
TGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	183564	21.99216704864984	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	103492	12.399018065627624	No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC	5333	0.6389282586479353	No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG	5203	0.6233534089152836	No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	5162	0.6184413409226781	No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	5127	0.6142481121485026	No Hit
NGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	5039	0.6037051369448615	No Hit
TGCAGTTGGTCGACAGTCCTCGGTCCGCAACGGTGTGGACATGGCAGAGG	4804	0.5755506008896834	No Hit
TGCAGGTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	4401	0.527268566718463	No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	3426	0.4104571937235752	No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC	2935	0.3516321843487137	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG	2930	0.35103315166668864	No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG	2929	0.3509133451302836	No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	2842	0.34049017646304747	No Hit
TGCAGATGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	2646	0.3170080953276649	No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	2600	0.31149699465303426	No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC	1649	0.19756097853186674	No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	1422	0.17036489476792877	No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	1315	0.15754559537259236	No Hit
TGCAGCTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	1108	0.13274564233675462	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA	1015	0.12160363445108838	No Hit
TTCCATATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	994	0.1190876971865831	No Hit
TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	972	0.11645195338567281	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0014376784368601583
6	0.0	0.0	0.0014376784368601583
7	0.0	0.0	0.0014376784368601583
8	0.0	0.0	0.0014376784368601583
9	0.0	0.0	0.0014376784368601583
10-11	0.0	0.0	0.0014376784368601583
12-13	0.0	0.0	0.0014376784368601583
14-15	0.0	0.0	0.0014376784368601583
16-17	0.0	0.0	0.0015574849732651714
18-19	0.0	0.0	0.0015574849732651714
20-21	0.0	0.0	0.0015574849732651714
22-23	0.0	0.0	0.004133325505972955
24-25	0.0	0.0	0.005391294138225593
26-27	0.0	0.0	0.005391294138225593
28-29	0.0	0.0	0.005391294138225593
30-31	0.0	0.0	0.005750713747440633
32-33	0.0	0.0	0.006469552965870712
34-35	0.0	0.0	0.007667618329920843
36-37	0.0	0.0	0.008027037939135884
38-39	0.0	0.0	0.00826665101194591
40-41	0.0	0.0	0.011201911153868732
42-43	0.0	0.0	0.01599417261006926
44-45	0.0	0.0	0.024799953035837728
46-47	0.0	0.0	0.05648878191496372
48-49	0.0	0.0	0.27130190168915236
50-51	0.0	0.0	0.3670273242767579
52-53	0.0	0.0	0.367865970031593
54-55	0.0	0.0	0.37014229422328826
56-57	0.0	0.0	0.38032584981771433
58-59	0.0	0.0	0.3812843021089545
60-61	0.0	0.0	0.3851780145421174
62-63	0.0	0.0	0.39074901848495047
64-65	0.0	0.0	0.39086882502135556
66-67	0.0	0.0	0.39092872828955805
68-69	0.0	0.0	0.39140795443517806
70-71	0.0	0.0	0.3916475675079881
72-73	0.0	0.0	0.3916475675079881
74-75	0.0	0.0	0.39188718058079813
76-77	0.0	0.0	0.3919470838490007
78-79	0.0	0.0	0.39500215052732845
80-81	0.0	0.0	0.3963799256959861
82-83	0.0	0.0	0.39655963550059364
84-85	0.0	0.0	0.39655963550059364
86-87	0.0	0.0	0.39745818452363124
88-89	0.0	0.0	0.39835673354666884
90-91	0.0	0.0	0.39835673354666884
92-93	0.0	0.0	0.39853644335127636
94-95	0.0	0.0	0.3988958629604914
96-97	0.0	0.0	0.3990755727650989
98-99	0.0	0.0	0.39913547603330146
100-101	0.0	0.0	0.39925528256970644
102-103	0.0	0.0	0.4111760329420052
104-105	0.0	0.0	0.422377944095874
106-107	0.0	0.0	0.4232764931189116
108-109	0.0	0.0	0.42333639638711407
110-111	0.0	0.0	0.4234562029235191
112-113	0.0	0.0	0.4235161061917216
114-115	0.0	0.0	0.4236359127281266
116-117	0.0	0.0	0.42693059247926446
118-119	0.0	0.0	0.4306445951078199
120-121	0.0	0.0	0.43465811407738786
122-123	0.0	0.0	0.7701164160114247
124-125	0.0	0.0	1.087663640752912
126-127	0.0	0.0	1.0880829636303297
128-129	0.0	0.0	1.0880829636303297
130-131	0.0	0.0	1.0883824799713422
132-133	0.0	0.0	1.0907786106994426
134-135	0.0	0.0	1.091078127040455
136-137	0.0	0.0	1.0916172564542777
138-139	0.0	0.0	1.0920964825998978
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCATTG	15	1.1187414E-4	148.49	1
TGCCGTT	15	1.1187414E-4	148.49	1
TTCCGCA	40	0.0	148.49	1
TGCCGCG	30	5.511538E-10	148.49	1
TCTCGCA	10	0.0066039274	148.49	1
TTCTAGA	35	9.094947E-12	148.49	1
GCCCGCA	10	0.0066039274	148.49	1
TTCAGAT	15	1.1187414E-4	148.49	1
TGAGTTG	20	1.8925311E-6	148.49	1
TGCGGTT	20	1.8925311E-6	148.49	1
TGCGTTG	25	3.2196112E-8	148.48999	1
TGCCGAG	50	0.0	148.48999	1
TCCCGTA	55	0.0	148.48999	1
TGCAGTT	20065	0.0	148.34198	1
TCCCGCA	11815	0.0	148.30147	1
TTCCAGA	23250	0.0	148.1068	1
TGCAGGT	560	0.0	147.1642	1
GTAGACA	45	0.0	144.97635	9
CTTAGGT	90	0.0	144.97635	145
GTGGGTC	45	0.0	144.97635	5
>>END_MODULE