FastQCFastQC Report
Wed 17 Jun 2015
000000000-AG1W8 l01n01 mis02.3410000000d163.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG1W8 l01n01 mis02.3410000000d163.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815124
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG20700625.395645325128445No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC19198323.55261285399522No Hit
TGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG16701120.48902988993086No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG8627310.584033840250072No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG53680.6585501101672875No Hit
TGCAGTTGGTCGACAGTCCTCGGCACGTGAGAAAACGAGAAAGTGAGCTG52950.649594417536473No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC51280.6291067371344727No Hit
TGCCGACGGAAAAATTGCGAACGCTCAACTCCGCTGAATTACTTACACAG50480.6192922794568679No Hit
TGCAGTTGGTCGACAGTCCTCGGTACGTGAGAAAACGAGAAAGTGAGCTG48420.5940200509370354No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC47840.5869045691207718No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC45570.559056045460568No Hit
TGCAGTTGGTCGACAGTCCTCGGTCCGCAACGGTGTGGACATGGCAGAGG45290.5556209852734062No Hit
NGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG45240.5550075816685559No Hit
TGCAGGTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG42120.516731196725897No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG35960.44115987260833933No Hit
TTCCAGATCTCGATGCCGACAGGGAAAGCAATAGAATCCAAAGTACCTGT26310.3227729768722305No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG24990.30657912170418244No Hit
TGCAGATGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG24540.3010584892605297No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG24410.2994636398879189No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG23120.28363782688278105No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC16990.20843454492813362No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC14220.17445198521942673No Hit
TTCCAGATCTCGATGCCGACGAGGGAAAGCAATAGAATCCAAAGTACCTG13360.16390144321600147No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA10860.13323126297348623No Hit
TCCCGCAGATGATGAAGATGAAGCAGCGTTCGCAATTTTTCCGTCGGCAC10560.1295508413443844No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG10110.12403020890073167No Hit
TGCAGCTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG9760.11973638366677952No Hit
TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG9550.11716008852640826No Hit
TTCCATATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC9420.11556523915379746No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGCA100.006608518148.455281
TCCCGGA2300.0148.455281
TGCAGGT4750.0148.455281
TGCGTTG201.8947085E-6148.455281
TGCCGCG100.006608518148.455281
TGAGTTG151.1197747E-4148.455261
TCCCGCA103000.0148.383211
TGCAGTT199000.0148.268771
TTCCAGA226600.0148.094961
AGATCCG351.0913936E-11144.976585
GCTGGTC1550.0144.976585
GCAGTCG351.0913936E-11144.976582
CTGACGG351.0913936E-11144.976583
GCTGACG351.0913936E-11144.976582
TCTCGTG351.0913936E-11144.976588
AGCTGGT1650.0144.976584
CGCCGGA1100.0144.976584
CTTAGGT550.0144.97658145
GTTGATC100.00709405144.976565
TTGATCG100.00709405144.976566
Produced by FastQC (version 0.11.2)