FastQCFastQC Report
Wed 17 Jun 2015
000000000-AG1W8 l01n01 mis01.3410000000d14a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AG1W8 l01n01 mis01.3410000000d14a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797461
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG18188322.80776113189234No Hit
TGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG17918722.469688172838545No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC15152119.00042760711809No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG11276814.14087961668345No Hit
TTCCAGATCTCGATGCCGACAGGGAAAGCAATAGAATCCAAAGTACCTGT81881.026758675345879No Hit
TGCAGTTGGTCGACAGTCCTCGGTCCGCAACGGTGTGGACATGGCAGAGG48630.6098103857116524No Hit
NGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG48450.6075532220384445No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG47450.5950134238539565No Hit
TGCAGGTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG45980.5765799205227591No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC41680.5226587883294607No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC38910.4879235473584288No Hit
TTCCAGATCTCGATGCCGACGACGGGAAAGCAATAGAATCCAAAGTACCT34690.43500559901988933No Hit
TGCAGTTGGTCGACAGTCCTCGGTACGTGAGAAAACGAGAAAGTGAGCTG34090.42748172010919655No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG32420.4065402571411016No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG31850.3993925721759434No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG31790.3986401842848741No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG29450.36929705653317213No Hit
TGCAGTTGGTCGACAGTCCTCGGCACGTGAGAAAACGAGAAAGTGAGCTG29040.3641557392775321No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG28830.3615223816587896No Hit
TGCAGATGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG27090.3397031328177804No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC20290.2544325051632619No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC15330.19223510616820133No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG13730.1721714290730205No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAACCCCCAAC12180.15273474188706407No Hit
TTCCAGATCTCGATGCCGACAGGCGGGAAAGCAATAGAATCCAAAGTACC11810.1480950165588035No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC11710.1468410367403547No Hit
TGCAGCTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG11290.14157432150286972No Hit
TTCCAGATCTCGATGCCGACGAGGGAAAGCAATAGAATCCAAAGTACCTG10210.12803133946362266No Hit
TTCCAGATCTCGATGCCGACAGGCAAGCAATAGAATCCAAAGTACCTGTC9830.12326621615351722No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA8940.11210579576932289No Hit
TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG8360.10483271282231983No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGTTG201.890312E-6148.524151
TGCGGTT201.890312E-6148.524151
TGCATTG253.2150638E-8148.524151
TCCCGGA2500.0148.524151
TCCCGCC100.006599332148.524151
ACCCGCA100.006599332148.524151
TTGCAGT100.006599332148.524151
TGCAGGT5350.0148.524151
TGCAGAC100.006599332148.524151
TCCCGCG359.094947E-12148.524141
TCCCGCA127400.0148.524141
TGCGTTG305.493348E-10148.524141
TGCCGAG700.0148.524141
TGCAGTT207750.0148.166691
TTCCAGA207600.0148.130661
CTTCAGT253.7143764E-8144.97612145
CATTGGT253.7143764E-8144.976123
CAGATCC650.0144.976124
CTCGCAG253.7143764E-8144.976122
CTTAGGT1050.0144.97612145
Produced by FastQC (version 0.11.2)