##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AG1W8 l01n01 mis01.3410000000d14a.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	797461
Sequences flagged as poor quality	0
Sequence length	151
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.04007844897744	34.0	34.0	34.0	34.0	34.0
2	33.52932118310488	34.0	34.0	34.0	34.0	34.0
3	33.88076282100316	34.0	34.0	34.0	34.0	34.0
4	33.75134332588051	34.0	34.0	34.0	34.0	34.0
5	33.79428837272293	34.0	34.0	34.0	34.0	34.0
6	36.38241870135342	38.0	38.0	38.0	34.0	38.0
7	37.07019779023676	38.0	38.0	38.0	36.0	38.0
8	37.19777017308684	38.0	38.0	38.0	36.0	38.0
9	37.41044515029575	38.0	38.0	38.0	37.0	38.0
10-14	37.27815980969602	38.0	38.0	38.0	36.4	38.0
15-19	37.22720659693703	38.0	38.0	38.0	36.2	38.0
20-24	37.4287091155555	38.0	38.0	38.0	36.6	38.0
25-29	37.10955745798227	38.0	38.0	38.0	35.8	38.0
30-34	36.90315639260101	38.0	38.0	38.0	35.4	38.0
35-39	36.91388694870345	38.0	38.0	38.0	35.6	38.0
40-44	36.95321300978982	38.0	38.0	38.0	36.0	38.0
45-49	36.71096166458297	38.0	37.6	38.0	34.6	38.0
50-54	36.90226757170571	38.0	38.0	38.0	35.2	38.0
55-59	36.486408990533704	38.0	37.4	38.0	34.2	38.0
60-64	36.40023324024623	38.0	37.0	38.0	34.0	38.0
65-69	36.402948106553175	38.0	37.2	38.0	34.0	38.0
70-74	36.21643365631674	38.0	37.0	38.0	34.0	38.0
75-79	35.92700558397213	38.0	36.8	38.0	32.0	38.0
80-84	35.935868211737	38.0	37.0	38.0	32.4	38.0
85-89	35.61698590902878	38.0	36.6	38.0	30.4	38.0
90-94	35.62541541216435	38.0	37.0	38.0	30.6	38.0
95-99	35.52510455056736	38.0	36.4	38.0	29.6	38.0
100-104	35.31201400444661	38.0	36.4	38.0	28.6	38.0
105-109	35.627531879301934	38.0	36.8	38.0	30.4	38.0
110-114	35.44547357174834	38.0	36.6	38.0	29.2	38.0
115-119	34.83685822880367	38.0	35.4	38.0	26.8	38.0
120-124	34.447411472159764	37.8	35.0	38.0	24.8	38.0
125-129	34.59655807619432	38.0	35.6	38.0	25.0	38.0
130-134	34.56071180910415	38.0	35.8	38.0	24.6	38.0
135-139	34.14675802327637	38.0	34.8	38.0	23.0	38.0
140-144	34.318250046083755	38.0	34.8	38.0	24.0	38.0
145-149	33.94134333842031	37.8	34.6	38.0	22.4	38.0
150-151	32.1502430839878	35.5	31.0	37.5	20.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7978872670596644
1101	2	0.31246570397935614
1101	3	0.012166007126872103
1101	4	0.08061947533472846
1101	5	0.07959745144096786
1101	6	1.2223870506886243
1101	7	0.36657414584604453
1101	8	0.23393853977482593
1101	9	0.0713409504750473
1101	10-14	0.18033927196201205
1101	15-19	0.1645505186212617
1101	20-24	0.07250618873780468
1101	25-29	0.20882513602665398
1101	30-34	0.09955098350570779
1101	35-39	0.1190597786551848
1101	40-44	0.24648324496929064
1101	45-49	0.22014040736734586
1101	50-54	0.17841343175856395
1101	55-59	0.2743408575825157
1101	60-64	0.385424951109691
1101	65-69	0.16571189135942177
1101	70-74	0.11210715290806661
1101	75-79	0.3162972422247776
1101	80-84	0.31186533515695913
1101	85-89	0.474456860447269
1101	90-94	0.33616122977858254
1101	95-99	0.3231854486662016
1101	100-104	0.5862250707815235
1101	105-109	0.2824748511924824
1101	110-114	0.3484916038655115
1101	115-119	0.5818234048264728
1101	120-124	0.28842130551448264
1101	125-129	0.026038416249775764
1101	130-134	0.19737481647520383
1101	135-139	0.20257464860390684
1101	140-144	0.2422848073226831
1101	145-149	0.2864328341563578
1101	150-151	0.284463402135799
1102	1	0.841949229193915
1102	2	0.28873704243491716
1102	3	0.03422133229140911
1102	4	0.051862689951434504
1102	5	0.07706737366797967
1102	6	1.00831829783246
1102	7	0.43520698987725126
1102	8	0.09030195871111601
1102	9	0.11837374667224054
1102	10-14	0.21689368529062136
1102	15-19	0.20506832184407386
1102	20-24	0.11899999807653217
1102	25-29	0.23399838485322277
1102	30-34	0.07692045429919148
1102	35-39	0.17760810168854135
1102	40-44	0.25528718102546577
1102	45-49	0.1348032416182079
1102	50-54	0.08388957240765649
1102	55-59	0.238163820472721
1102	60-64	0.361791872179289
1102	65-69	0.24869528185156042
1102	70-74	0.10151490799790963
1102	75-79	0.13638052466329498
1102	80-84	0.3542166332952732
1102	85-89	0.39880411831959606
1102	90-94	0.3149474416234739
1102	95-99	0.35038916605258663
1102	100-104	0.5905886434700136
1102	105-109	0.2710119417738781
1102	110-114	0.212795981959232
1102	115-119	0.6065937374864276
1102	120-124	0.3948198739107198
1102	125-129	0.22378398199379035
1102	130-134	0.29708217479895893
1102	135-139	0.402625472245866
1102	140-144	0.2693778231292683
1102	145-149	0.31151126912985916
1102	150-151	0.5413715736378322
1103	1	0.9180506829795334
1103	2	0.3482739410939857
1103	3	0.04572961350026361
1103	4	0.0855351316341455
1103	5	0.10808300588176678
1103	6	1.325326751744619
1103	7	0.47766806323682687
1103	8	0.35794163464448303
1103	9	0.23919894357110394
1103	10-14	0.22465695523101203
1103	15-19	0.23843100903906844
1103	20-24	0.12130182531168288
1103	25-29	0.2948125184811232
1103	30-34	0.33837042676926643
1103	35-39	0.2879056643490685
1103	40-44	0.32065290164282345
1103	45-49	0.38244378961776704
1103	50-54	0.3557979268016709
1103	55-59	0.5675704050853625
1103	60-64	0.6065350230399105
1103	65-69	0.4464989575250158
1103	70-74	0.408089774024603
1103	75-79	0.46606198111641106
1103	80-84	0.6010407734551251
1103	85-89	0.6945630552880218
1103	90-94	0.5926818820818838
1103	95-99	0.6215791773039498
1103	100-104	0.7837355712321568
1103	105-109	0.50369740973143
1103	110-114	0.5765181143149363
1103	115-119	1.0180053219451892
1103	120-124	0.727105815861897
1103	125-129	0.33333661204279963
1103	130-134	0.4416989615599647
1103	135-139	0.4906058353673828
1103	140-144	0.5767302815009359
1103	145-149	0.6195447085930823
1103	150-151	0.6796746439947654
2116	1	0.7688353620721813
2116	2	0.29737883183498326
2116	3	-0.011948626119654193
2116	4	-0.17002679928123143
2116	5	0.03593194685340961
2116	6	1.2397302627858906
2116	7	0.11509265870495256
2116	8	0.16323363000877578
2116	9	0.14951518375387707
2116	10-14	0.213963976439544
2116	15-19	0.268008900980945
2116	20-24	0.10802193138057703
2116	25-29	0.295091849554872
2116	30-34	0.36626643275442206
2116	35-39	0.24606473622874603
2116	40-44	0.1917198921563994
2116	45-49	0.36626788970148993
2116	50-54	0.21176386519151436
2116	55-59	0.39858952650348556
2116	60-64	0.4801742454577038
2116	65-69	0.38798456008829163
2116	70-74	0.33340725529036774
2116	75-79	0.27330986209829433
2116	80-84	0.44540599446403206
2116	85-89	0.5669304735382283
2116	90-94	0.46033689946577994
2116	95-99	0.7372400714780198
2116	100-104	0.718854898739977
2116	105-109	0.42032892694665236
2116	110-114	0.46116971959461495
2116	115-119	0.8303552339331688
2116	120-124	0.7228630600283736
2116	125-129	0.49693534194292965
2116	130-134	0.5775240457700761
2116	135-139	0.5556881401459037
2116	140-144	0.6327133940322014
2116	145-149	0.7589117737464406
2116	150-151	1.3631607893133477
1117	1	0.8921145910593964
1117	2	0.3867221803139529
1117	3	0.0641911526209924
1117	4	-0.008054136237163334
1117	5	0.1083628008302
1117	6	1.2112271858766874
1117	7	0.42660141462329193
1117	8	0.19776995280690102
1117	9	0.1975774097291989
1117	10-14	0.19134078951013578
1117	15-19	0.2011365276409265
1117	20-24	0.10372123322615323
1117	25-29	0.20105713039310302
1117	30-34	0.1507654286802662
1117	35-39	0.039492654440984154
1117	40-44	0.15015181588416482
1117	45-49	0.29507153979214706
1117	50-54	0.1528869452998478
1117	55-59	0.2705016106631035
1117	60-64	0.38705820960014137
1117	65-69	0.2850542300958523
1117	70-74	0.28557103676105555
1117	75-79	0.20685209977003183
1117	80-84	0.4298983016959852
1117	85-89	0.3354029628742836
1117	90-94	0.35328272566578534
1117	95-99	-0.2353841605598106
1117	100-104	0.5041644587537348
1117	105-109	0.3839097368198736
1117	110-114	0.416337648715583
1117	115-119	0.8167330047631296
1117	120-124	0.6030883546004304
1117	125-129	0.3794945946398087
1117	130-134	0.38441863701497425
1117	135-139	0.2240046112966212
1117	140-144	0.3620995765547761
1117	145-149	-0.756815831835361
1117	150-151	-0.2529482258358797
2117	1	-30.959255590083195
2117	2	-10.544522416806732
2117	3	-1.69844176155555
2117	4	-0.38860150797740545
2117	5	0.06244030351445673
2117	6	1.2376369100740732
2117	7	0.06177345641228982
2117	8	0.26508134381003856
2117	9	0.24659230653040254
2117	10-14	0.3973828660941052
2117	15-19	0.3993665851313395
2117	20-24	0.3250240944620799
2117	25-29	0.4479001457429419
2117	30-34	0.5507045539083322
2117	35-39	0.3431317346820606
2117	40-44	0.19293476131316822
2117	45-49	0.5193138593399382
2117	50-54	0.3819392963871877
2117	55-59	0.5601326321743727
2117	60-64	0.6591001689113725
2117	65-69	0.5684357823149568
2117	70-74	0.5343633586567265
2117	75-79	0.5715822009335838
2117	80-84	0.7237415557274858
2117	85-89	0.7691792643888249
2117	90-94	0.6170145730253438
2117	95-99	0.10052818366148131
2117	100-104	0.9834282292378305
2117	105-109	0.8015273143007775
2117	110-114	0.9417720870484914
2117	115-119	1.2311333675787068
2117	120-124	1.0482832256454202
2117	125-129	0.6160513793423803
2117	130-134	0.9213477991693608
2117	135-139	0.7336566202492563
2117	140-144	0.7549361097876002
2117	145-149	0.9887083517748181
2117	150-151	1.3932875989430684
1116	1	0.20669165212304108
1116	2	0.23446616812417176
1116	3	-0.07078330162889301
1116	4	-0.30094955515516375
1116	5	0.07463773258010775
1116	6	1.2247199309282095
1116	7	-0.09808122663017116
1116	8	0.21314485214428203
1116	9	0.11111816096471472
1116	10-14	0.2472128146025554
1116	15-19	0.248410656317553
1116	20-24	0.07904333689945986
1116	25-29	0.03787905418244719
1116	30-34	0.1068044618290358
1116	35-39	0.05923239632142696
1116	40-44	0.10892553077199096
1116	45-49	0.2530582205822185
1116	50-54	0.13716996570192208
1116	55-59	0.29624517744460377
1116	60-64	0.3650805998625799
1116	65-69	0.20126118464369114
1116	70-74	0.16454778105071455
1116	75-79	0.06978551151717483
1116	80-84	0.2077046084387959
1116	85-89	0.1680227082298842
1116	90-94	0.22254631525859025
1116	95-99	-0.819165555323238
1116	100-104	0.4392790331415952
1116	105-109	0.269499572429325
1116	110-114	0.32602765797152244
1116	115-119	0.7588986294394076
1116	120-124	0.5552753982771463
1116	125-129	0.16443446438822917
1116	130-134	0.31062504578484607
1116	135-139	0.39715189003656093
1116	140-144	0.17744355589986327
1116	145-149	0.2842400814460859
1116	150-151	0.34598082414289877
2118	1	0.830218094127332
2118	2	0.36266177651868503
2118	3	0.0630779000478654
2118	4	0.04245326334457644
2118	5	0.09273291642372072
2118	6	1.3092490100403325
2118	7	0.49875558431232037
2118	8	0.21141649100459858
2118	9	0.14657871660032384
2118	10-14	0.23209915983471063
2118	15-19	0.21191353077625052
2118	20-24	0.11143157814623095
2118	25-29	0.2677619871467485
2118	30-34	0.2542535263315102
2118	35-39	0.15383650064858045
2118	40-44	0.20596059950322143
2118	45-49	0.33441517507451124
2118	50-54	0.088031610805956
2118	55-59	0.27243078694488787
2118	60-64	0.4217623117822882
2118	65-69	0.33831698387947995
2118	70-74	0.389302639483482
2118	75-79	0.32683961299612463
2118	80-84	0.4324624602849383
2118	85-89	0.19256941273864925
2118	90-94	0.36088079346084356
2118	95-99	0.541421332409243
2118	100-104	0.6948099786692907
2118	105-109	0.33222838146406275
2118	110-114	0.6150761211106612
2118	115-119	0.8817244563419351
2118	120-124	0.5227399883191381
2118	125-129	0.4292329283650673
2118	130-134	0.32992702199614143
2118	135-139	0.34039185791322524
2118	140-144	0.2140568437511945
2118	145-149	0.22087403278524675
2118	150-151	0.94860251812446
1119	1	0.8429162701623198
1119	2	0.31078426378928015
1119	3	0.03384370498111622
1119	4	-0.06381597589958687
1119	5	0.08746984649238243
1119	6	1.3524649870079912
1119	7	0.2599367216174642
1119	8	0.33819087764682365
1119	9	0.1586022133828422
1119	10-14	0.16943236474147483
1119	15-19	0.19311307002398337
1119	20-24	0.04328637872662
1119	25-29	0.15459320693955902
1119	30-34	0.1272064971413016
1119	35-39	0.11268814730136967
1119	40-44	0.0038936356082288626
1119	45-49	0.12859982695717065
1119	50-54	0.1343195774572763
1119	55-59	0.25288813503320284
1119	60-64	0.2611249202136605
1119	65-69	0.1671308458552332
1119	70-74	0.19612433284044783
1119	75-79	0.21258562836420936
1119	80-84	0.20678559294297116
1119	85-89	0.3183407087869412
1119	90-94	0.40252389693262103
1119	95-99	0.3781701964719275
1119	100-104	0.5552863972220052
1119	105-109	0.33623933642549275
1119	110-114	0.4139299140703301
1119	115-119	0.7490105172662638
1119	120-124	0.7186236092268032
1119	125-129	0.18391598964960565
1119	130-134	0.4408419600721132
1119	135-139	0.4538220213536093
1119	140-144	0.39149278110893704
1119	145-149	0.6220561288914155
1119	150-151	1.0430881807505301
2119	1	0.8711429270623086
2119	2	0.32905214901508373
2119	3	0.0541471895666632
2119	4	0.0328636167354972
2119	5	0.04462993159198447
2119	6	1.226112551771294
2119	7	0.4727417442794746
2119	8	0.20893031830333086
2119	9	0.0826925603886437
2119	10-14	0.1361707937243608
2119	15-19	0.11164848866520316
2119	20-24	0.0976839647123171
2119	25-29	0.11805769903302377
2119	30-34	0.027615542343674804
2119	35-39	0.10383609056714249
2119	40-44	-0.19225684121735753
2119	45-49	0.14370036858763058
2119	50-54	0.050803381490140964
2119	55-59	0.2528070086098779
2119	60-64	0.23565656170662663
2119	65-69	0.14999277953460677
2119	70-74	0.12774289687649087
2119	75-79	0.08234901400527406
2119	80-84	0.18822960827210267
2119	85-89	0.39917483194331993
2119	90-94	0.26536449445016785
2119	95-99	0.28746052842515013
2119	100-104	0.5884570401335907
2119	105-109	0.3429303847766647
2119	110-114	0.49639190410937317
2119	115-119	0.9470015224725259
2119	120-124	0.4667383169135064
2119	125-129	0.5513094685554236
2119	130-134	0.6234955204894135
2119	135-139	0.25656430449794954
2119	140-144	0.3258189723415015
2119	145-149	0.35043619962105765
2119	150-151	0.4169508136749016
1118	1	0.8776464600306966
1118	2	0.40658724119737855
1118	3	0.053134693815302114
1118	4	0.1003396185172889
1118	5	0.07278263351585679
1118	6	1.2343843633913494
1118	7	0.3694692887661688
1118	8	0.39408256211298465
1118	9	0.04288919061024643
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2111	3	0.08324513439006864
2111	4	0.1250338287538213
2111	5	0.03395785941702911
2111	6	0.9831444709170754
2111	7	0.3549857037118116
2111	8	-0.0110087159964678
2111	9	-0.07914062726536031
2111	10-14	-0.11206730632439843
2111	15-19	-0.09877313219666206
2111	20-24	-0.09580271990439115
2111	25-29	0.0022940245758036326
2111	30-34	-0.007265798550328384
2111	35-39	-0.17300827559601828
2111	40-44	-0.12167103462972051
2111	45-49	-0.13862168667681374
2111	50-54	-0.2896967305145637
2111	55-59	-0.31577773503110507
2111	60-64	-0.24926742882348663
2111	65-69	-0.3438659364082781
2111	70-74	-0.13874134120597859
2111	75-79	-0.3718076783858777
2111	80-84	-0.14340930611676583
2111	85-89	-0.4444498161203114
2111	90-94	-0.593173734294183
2111	95-99	-0.35637468849063936
2111	100-104	-0.37838016651289763
2111	105-109	-0.33481651972065407
2111	110-114	-0.5530988038921549
2111	115-119	-2.3482847044621806
2111	120-124	-1.1953509442507269
2111	125-129	-0.544447529542694
2111	130-134	-0.23435864498765824
2111	135-139	-0.13953512054454364
2111	140-144	-0.5825807658749511
2111	145-149	-0.9806779178038028
2111	150-151	-2.1712052374343784
2110	1	0.9861467087673788
2110	2	0.34020640352206044
2110	3	0.09329715814122608
2110	4	0.11172237626521309
2110	5	0.09924494336321033
2110	6	1.1774177726548274
2110	7	0.1371278474898432
2110	8	-0.050837754873548135
2110	9	-0.08930977255054273
2110	10-14	-0.06778801232211151
2110	15-19	-0.11902753106357977
2110	20-24	-0.19983742056354004
2110	25-29	-0.15427522239628644
2110	30-34	-0.02584045671888191
2110	35-39	-0.2578688192578369
2110	40-44	-0.17545524047308447
2110	45-49	-0.08827767621758653
2110	50-54	-0.2083906892224192
2110	55-59	-0.2851476393364578
2110	60-64	-0.18381256147042535
2110	65-69	-0.2345751118363637
2110	70-74	-0.2654296163417129
2110	75-79	-0.3373004059557374
2110	80-84	-0.37222165744505276
2110	85-89	-0.1809073890715993
2110	90-94	-0.43002280186151154
2110	95-99	-0.7390646705138479
2110	100-104	-0.12684324145251935
2110	105-109	-0.2078360762930771
2110	110-114	-0.6133759915226662
2110	115-119	-6.146367089020302
2110	120-124	-3.078633888086866
2110	125-129	-0.37265793066124786
2110	130-134	-0.608619145069369
2110	135-139	-0.6259137953461575
2110	140-144	-0.6163410026601923
2110	145-149	-0.5279118521794217
2110	150-151	-0.9114001676154189
2109	1	0.9385305615889692
2109	2	0.3493748252775859
2109	3	0.09530242611486983
2109	4	0.06222232844017128
2109	5	0.060418001440858404
2109	6	1.05261403580446
2109	7	0.37411672861843925
2109	8	-0.030917947545312074
2109	9	-0.13896096555978232
2109	10-14	-0.03299218013928851
2109	15-19	-0.1407894598024555
2109	20-24	-0.32408349936115854
2109	25-29	-0.11421529671459751
2109	30-34	-0.17973634791600546
2109	35-39	-0.43357320093416263
2109	40-44	-0.2605371656747124
2109	45-49	-0.5423500688954874
2109	50-54	-0.26323270415466027
2109	55-59	-0.6321315935832317
2109	60-64	-0.44254681304459353
2109	65-69	-0.39767037571329666
2109	70-74	-0.4494460356716985
2109	75-79	-0.435227054972259
2109	80-84	-0.46990092465710376
2109	85-89	-0.6358255631294796
2109	90-94	-0.6094279395344699
2109	95-99	-0.2989701393526687
2109	100-104	-0.39871841628463756
2109	105-109	-0.4914550063476071
2109	110-114	-1.1783597129607415
2109	115-119	-5.039374751855551
2109	120-124	-1.601028325583144
2109	125-129	-0.6510560353457606
2109	130-134	-0.8963008576751506
2109	135-139	-0.981451303715545
2109	140-144	-0.5057472141407189
2109	145-149	-0.3708051409723012
2109	150-151	-1.5851916377933435
2108	1	0.8652297836979557
2108	2	0.14205714842768913
2108	3	0.04200109707100097
2108	4	0.05432323636243552
2108	5	-0.048472706479259386
2108	6	0.8150577148722391
2108	7	-0.16919980305830506
2108	8	-0.4123935470388602
2108	9	-0.5189012471274452
2108	10-14	-0.23500230604199146
2108	15-19	-0.35072016253764815
2108	20-24	-0.44785768183052
2108	25-29	-0.2374671087571869
2108	30-34	-4.715828439433913
2108	35-39	-1.000671212752323
2108	40-44	-0.6549413821981815
2108	45-49	-0.3942990831739195
2108	50-54	-0.37268472043000145
2108	55-59	-0.42397509270981004
2108	60-64	-0.6429634437757556
2108	65-69	-0.701400659878523
2108	70-74	-0.6378212013365072
2108	75-79	-0.924996075997214
2108	80-84	-0.8989468331381758
2108	85-89	-1.3383181519097462
2108	90-94	-1.1486939059988543
2108	95-99	-1.0079890923765902
2108	100-104	-0.7966308738190762
2108	105-109	-0.851272646198808
2108	110-114	-1.207868691904153
2108	115-119	-1.4514007540223943
2108	120-124	-1.2510050291685388
2108	125-129	-0.7486944474335644
2108	130-134	-0.8598576103437807
2108	135-139	-1.2860703728898173
2108	140-144	-1.3022430047108031
2108	145-149	-1.2304576901308053
2108	150-151	-1.6526917870639082
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	11.0
18	37.0
19	98.0
20	296.0
21	720.0
22	1676.0
23	2788.0
24	3926.0
25	4853.0
26	5841.0
27	8137.0
28	9758.0
29	12139.0
30	15821.0
31	20989.0
32	30252.0
33	34965.0
34	39119.0
35	59438.0
36	118064.0
37	428531.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.11344146458969007	0.0869760719447567	99.62961297575076	0.16996948771479042
2	54.98362929347015	0.04250991584541438	26.94489134891863	18.028969441765806
3	0.06909428799652897	0.111980397787478	0.09630565005686799	99.72261966415913
4	0.3385745509811765	28.343204244470886	0.1657761319989316	71.152445072549
5	72.59941238505706	27.02602384317227	0.19148271827713204	0.18308105349352508
6	27.95196254111486	27.152926600799287	27.38328269344833	17.511828164637517
7	0.6371471457538362	44.410447658255386	28.337937529233404	26.61446766675737
8	72.62750153299032	0.2897947360435181	26.757922957987912	0.3247807729782397
9	54.74048260667293	17.87648549584243	0.6535742813755155	26.729457616109126
10-14	18.35282226967839	41.387328032342644	23.4621128807553	16.79773681722366
15-19	30.665474549852593	21.83399564367411	19.942116291580405	27.558413514892894
20-24	34.97929052329832	23.508510134037902	11.34841703857618	30.1637823040876
25-29	23.485687701342133	44.38993254842556	5.839884332901546	26.284495417330756
30-34	24.221548138404263	18.22110422954853	33.65245447739764	23.90489315464957
35-39	42.167253320225065	21.97411534858758	20.706717946081376	15.151913385105981
40-44	15.187752128317248	30.966830979822213	27.039792541578834	26.80562435028171
45-49	16.852435416904402	27.895056936953655	21.11318296443337	34.13932468170857
50-54	31.08651081369496	24.81618536831268	17.199662428632873	26.897641389359478
55-59	20.871365496243705	43.81781679605648	29.50233302945222	5.808484678247589
60-64	35.523342207330515	21.58046600398013	20.796227025522253	22.0999647631671
65-69	20.40874726162157	33.89043476734285	25.1667730459546	20.534044925080977
70-74	27.095644802692547	13.572601042558821	27.5023856966046	31.829368458144035
75-79	29.688147758949967	18.621650463157447	25.971025542314923	25.719176235577663
80-84	31.76972917797861	19.165451351225954	28.82468233556249	20.240137135232946
85-89	29.720575677055056	20.042534995441784	36.807793735367625	13.429095592135539
90-94	28.659733830243738	30.815701332102762	27.9925162484435	12.532048589210005
95-99	17.8826550765492	28.094364489297906	30.081746944364678	23.941233489788218
100-104	11.304377267352258	34.64708618979486	32.9044805952893	21.14405594756358
105-109	17.904022892655565	25.519066136149604	33.6096436063958	22.96726736479903
110-114	36.54044523807434	29.08997430595352	13.195629629536743	21.1739508264354
115-119	9.53425935562993	38.51270469653061	18.097537058238586	33.855498889600874
120-124	10.596305023066959	21.518118127406858	42.20983847485959	25.675738374666597
125-129	17.89002847788168	29.26114255117178	23.25951990128671	29.58930906965983
130-134	13.591159943871864	30.480362049053184	49.52332465161305	6.405153355461897
135-139	25.01273416505635	5.489647769608796	46.90330937813887	22.594308687195987
140-144	12.99092996397316	33.92720145561977	20.895542227143395	32.186326353263674
145-149	23.658009607993367	19.56893189761004	31.478454745749325	25.29460374864727
150-151	34.90766319606852	19.3547396048208	29.828606038414417	15.908991160696258
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.5
26	1.0
27	1.5
28	2.0
29	2.0
30	6.0
31	11.5
32	22.5
33	73.0
34	363.5
35	3183.0
36	72578.5
37	78034.5
38	9176.5
39	1160.5
40	627.0
41	641.5
42	689.5
43	825.5
44	1147.5
45	1900.5
46	6941.0
47	53216.0
48	77784.0
49	56651.5
50	104210.0
51	90491.0
52	16009.5
53	4721.5
54	1782.5
55	589.0
56	266.0
57	227.0
58	301.5
59	556.0
60	1559.5
61	8484.5
62	93379.0
63	96619.5
64	11619.5
65	1269.0
66	238.0
67	60.5
68	21.0
69	9.0
70	3.5
71	2.0
72	1.5
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.393345881491383
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	797461.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	1.7465644306577603
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.52453613962189	1.3016190403273238
2	9.439638796252693	0.3297387471958398
3	3.4790010397773887	0.1822889841088965
4	1.9743583560259497	0.13793376312027417
5	1.3141261215762656	0.11476029706716703
6	0.8674478887465771	0.09090321767603647
7	0.5214520844765711	0.06375247641273872
8	0.4896905426794651	0.06842208670987596
9	0.5686606046061506	0.08938821466092953
>10	3.9272430755678545	1.4815360767135715
>50	1.2142516923662294	1.5466373297637064
>100	1.3669420249392283	4.515713671817689
>500	0.10906452326642777	1.279699841697091
>1k	0.1672322690085226	8.342113982443376
>5k	0.007270968217761852	1.0398116804421733
>10k+	0.029083872871047408	79.41568058984332
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	181883	22.80776113189234	No Hit
TGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	179187	22.469688172838545	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	151521	19.00042760711809	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	112768	14.14087961668345	No Hit
TTCCAGATCTCGATGCCGACAGGGAAAGCAATAGAATCCAAAGTACCTGT	8188	1.026758675345879	No Hit
TGCAGTTGGTCGACAGTCCTCGGTCCGCAACGGTGTGGACATGGCAGAGG	4863	0.6098103857116524	No Hit
NGCAGTTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	4845	0.6075532220384445	No Hit
NGCCGACGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	4745	0.5950134238539565	No Hit
TGCAGGTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	4598	0.5765799205227591	No Hit
TTCCAGATCTCGATGCCGACAGGCCGAGAATTTTCCGGCACATATTCGCC	4168	0.5226587883294607	No Hit
NTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	3891	0.4879235473584288	No Hit
TTCCAGATCTCGATGCCGACGACGGGAAAGCAATAGAATCCAAAGTACCT	3469	0.43500559901988933	No Hit
TGCAGTTGGTCGACAGTCCTCGGTACGTGAGAAAACGAGAAAGTGAGCTG	3409	0.42748172010919655	No Hit
TCCCGCAGATGATGAAGATGAAGCCGCAGGGCCTAGTGGCCGACCTCTTG	3242	0.4065402571411016	No Hit
NCCCGCAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	3185	0.3993925721759434	No Hit
TGCCGGCGGAAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	3179	0.3986401842848741	No Hit
TCCCGCAGATGATGAAGATGAAGCAGCAGGACCTAGTGGCCGACCTCTTG	2945	0.36929705653317213	No Hit
TGCAGTTGGTCGACAGTCCTCGGCACGTGAGAAAACGAGAAAGTGAGCTG	2904	0.3641557392775321	No Hit
TCCCGGAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	2883	0.3615223816587896	No Hit
TGCAGATGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	2709	0.3397031328177804	No Hit
TTCCAGATCTCGATGCCGACGGGCAGAGAATTTTCCGGCACATATTCGCC	2029	0.2544325051632619	No Hit
TGCCGACGGAAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAGC	1533	0.19223510616820133	No Hit
TCCCGAAGATGATGAAGATGAAGCAGCAGGGCCTAGTGGCCGACCTCTTG	1373	0.1721714290730205	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTTTAATTAACCCCCAAC	1218	0.15273474188706407	No Hit
TTCCAGATCTCGATGCCGACAGGCGGGAAAGCAATAGAATCCAAAGTACC	1181	0.1480950165588035	No Hit
TTCCAAATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCC	1171	0.1468410367403547	No Hit
TGCAGCTGGTCGACAGTCCTCGGTACGCAACGGTGTGGACATGGCAGAGG	1129	0.14157432150286972	No Hit
TTCCAGATCTCGATGCCGACGAGGGAAAGCAATAGAATCCAAAGTACCTG	1021	0.12803133946362266	No Hit
TTCCAGATCTCGATGCCGACAGGCAAGCAATAGAATCCAAAGTACCTGTC	983	0.12326621615351722	No Hit
TTCCAGATCTCGATGCCGACAGGCAGAGAATTTTCCGGCACATATTCGCA	894	0.11210579576932289	No Hit
TGCCGACGGACAAATTGCGAACGCTCAACTACGCTGAATTACTTACACAG	836	0.10483271282231983	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	1.2539798184488019E-4
4	0.0	0.0	1.2539798184488019E-4
5	0.0	0.0	0.0051413172556400875
6	0.0	0.0	0.0051413172556400875
7	0.0	0.0	0.0051413172556400875
8	0.0	0.0	0.0051413172556400875
9	0.0	0.0	0.0051413172556400875
10-11	0.0	0.0	0.005204016246562528
12-13	0.0	0.0	0.005580210192097168
14-15	0.0	0.0	0.0058937051467093686
16-17	0.0	0.0	0.0058937051467093686
18-19	0.0	0.0	0.00608180211947669
20-21	0.0	0.0	0.006144501110399129
22-23	0.0	0.0	0.01398187497570414
24-25	0.0	0.0	0.015800145712454902
26-27	0.0	0.0	0.016176339657989543
28-29	0.0	0.0	0.016176339657989543
30-31	0.0	0.0	0.016427135621679304
32-33	0.0	0.0	0.017743814431050546
34-35	0.0	0.0	0.019248590213189108
36-37	0.0	0.0	0.07680626387998912
38-39	0.0	0.0	0.1285329313910022
40-41	0.0	0.0	0.13348615167387495
42-43	0.0	0.0	0.19292479506834817
44-45	0.0	0.0	0.3181973789313835
46-47	0.0	0.0	1.2909722230930416
48-49	0.0	0.0	3.582494943326382
50-51	0.0	0.0	4.137318313999055
52-53	0.0	0.0	4.1665987427598346
54-55	0.0	0.0	4.20403004034053
56-57	0.0	0.0	4.262841693825779
58-59	0.0	0.0	4.34917820432598
60-61	0.0	0.0	4.3546957155271535
62-63	0.0	0.0	4.358394955991578
64-65	0.0	0.0	4.368238697566401
66-67	0.0	0.0	4.368489493530091
68-69	0.0	0.0	4.368489493530091
70-71	0.0	0.0	4.368552192521014
72-73	0.0	0.0	4.368614891511936
74-75	0.0	0.0	4.368677590502859
76-77	0.0	0.0	4.370433162248687
78-79	0.0	0.0	4.376514964368163
80-81	0.0	0.0	4.378207837123069
82-83	0.0	0.0	4.378270536113992
84-85	0.0	0.0	4.378521332077682
86-87	0.0	0.0	4.378772128041371
88-89	0.0	0.0	4.378772128041371
90-91	0.0	0.0	4.378772128041371
92-93	0.0	0.0	4.378772128041371
94-95	0.0	0.0	4.378772128041371
96-97	0.0	0.0	4.378772128041371
98-99	0.0	0.0	4.378897526023216
100-101	0.0	0.0	4.378897526023216
102-103	0.0	0.0	4.390935732280324
104-105	0.0	0.0	4.40159456073714
106-107	0.0	0.0	4.40159456073714
108-109	0.0	0.0	4.402158851655441
110-111	0.0	0.0	4.402723142573743
112-113	0.0	0.0	4.403224734501123
114-115	0.0	0.0	4.40366362743758
116-117	0.0	0.0	4.40573269413802
118-119	0.0	0.0	4.410309720475359
120-121	0.0	0.0	4.430686892525152
122-123	0.0	0.0	7.539177464477886
124-125	0.0	0.0	10.482769690304604
126-127	0.0	0.0	10.496688866289386
128-129	0.0	0.0	10.496876963262153
130-131	0.0	0.0	10.497253157207687
132-133	0.0	0.0	10.4975666521623
134-135	0.0	0.0	10.49781744812599
136-137	0.0	0.0	10.497942846107835
138-139	0.0	0.0	10.49806824408968
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAGTTG	20	1.890312E-6	148.52415	1
TGCGGTT	20	1.890312E-6	148.52415	1
TGCATTG	25	3.2150638E-8	148.52415	1
TCCCGGA	250	0.0	148.52415	1
TCCCGCC	10	0.006599332	148.52415	1
ACCCGCA	10	0.006599332	148.52415	1
TTGCAGT	10	0.006599332	148.52415	1
TGCAGGT	535	0.0	148.52415	1
TGCAGAC	10	0.006599332	148.52415	1
TCCCGCG	35	9.094947E-12	148.52414	1
TCCCGCA	12740	0.0	148.52414	1
TGCGTTG	30	5.493348E-10	148.52414	1
TGCCGAG	70	0.0	148.52414	1
TGCAGTT	20775	0.0	148.16669	1
TTCCAGA	20760	0.0	148.13066	1
CTTCAGT	25	3.7143764E-8	144.97612	145
CATTGGT	25	3.7143764E-8	144.97612	3
CAGATCC	65	0.0	144.97612	4
CTCGCAG	25	3.7143764E-8	144.97612	2
CTTAGGT	105	0.0	144.97612	145
>>END_MODULE