FastQCFastQC Report
Fri 12 Jun 2015
000000000-AFM8U l01n02 ve19.3420000000c8c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFM8U l01n02 ve19.3420000000c8c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440329
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGC601.2148455E-535.01
TTAAGGC450.004305603431.1111093
AGGACTG703.4860044E-530.05
GGGTATG604.979359E-429.1666661
ACCGGCA1800.029.16666453
TACCGGC1700.028.8235352
GGCTATC657.951499E-426.9230771
TGGGGTC1951.8189894E-1225.12820466
GGACTAA700.001225352825.01
CTAAGCT700.001225352825.04
CCGGCAG2001.8189894E-1224.49999854
ATACCGG2051.8189894E-1223.9024451
GCTACAC1202.1638898E-623.3333323
GAGTCCT2300.022.82608638
GGGGTCT2259.094947E-1221.77777967
GTCTATA1154.1120933E-521.3043481
TCCTGAC2501.8189894E-1221.041
CTACGGA1003.928318E-421.01
TAGACAG850.00378710620.5882365
CCTGTAC850.00378710620.5882363
Produced by FastQC (version 0.11.2)