##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AFM8U l01n02 ve18.3420000000c8a0.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1521018 Sequences flagged as poor quality 0 Sequence length 76 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.424039031753736 34.0 34.0 34.0 34.0 34.0 2 33.62028391511475 34.0 34.0 34.0 34.0 34.0 3 33.64379185519172 34.0 34.0 34.0 34.0 34.0 4 33.67208606341279 34.0 34.0 34.0 34.0 34.0 5 33.667809322440625 34.0 34.0 34.0 34.0 34.0 6 37.42087207383476 38.0 38.0 38.0 37.0 38.0 7 37.4405871593893 38.0 38.0 38.0 37.0 38.0 8 37.3393523285063 38.0 38.0 38.0 37.0 38.0 9 37.403806529574275 38.0 38.0 38.0 37.0 38.0 10-11 37.385440540480126 38.0 38.0 38.0 37.0 38.0 12-13 37.41697073933379 38.0 38.0 38.0 37.0 38.0 14-15 37.392385560197184 38.0 38.0 38.0 37.0 38.0 16-17 37.394251087100876 38.0 38.0 38.0 37.0 38.0 18-19 37.378355154245384 38.0 38.0 38.0 37.0 38.0 20-21 37.38253426323686 38.0 38.0 38.0 37.0 38.0 22-23 37.3381054662075 38.0 38.0 38.0 37.0 38.0 24-25 37.36724121608029 38.0 38.0 38.0 37.0 38.0 26-27 37.35120721779755 38.0 38.0 38.0 37.0 38.0 28-29 37.38150173107748 38.0 38.0 38.0 37.0 38.0 30-31 37.350144442735065 38.0 38.0 38.0 37.0 38.0 32-33 37.34259818095512 38.0 38.0 38.0 37.0 38.0 34-35 37.33189712416289 38.0 38.0 38.0 37.0 38.0 36-37 37.29360566410128 38.0 38.0 38.0 37.0 38.0 38-39 37.28095196769532 38.0 38.0 38.0 37.0 38.0 40-41 37.283372057398395 38.0 38.0 38.0 37.0 38.0 42-43 37.27809828680529 38.0 38.0 38.0 37.0 38.0 44-45 37.292623427204674 38.0 38.0 38.0 37.0 38.0 46-47 37.25138032554513 38.0 38.0 38.0 37.0 38.0 48-49 37.24104316977183 38.0 38.0 38.0 37.0 38.0 50-51 37.22622546215758 38.0 38.0 38.0 37.0 38.0 52-53 37.212186180571166 38.0 38.0 38.0 37.0 38.0 54-55 37.19687143741889 38.0 38.0 38.0 37.0 38.0 56-57 37.1911676916381 38.0 38.0 38.0 37.0 38.0 58-59 37.16623143184367 38.0 38.0 38.0 37.0 38.0 60-61 37.1741570448213 38.0 38.0 38.0 37.0 38.0 62-63 37.112803727503554 38.0 38.0 38.0 37.0 38.0 64-65 37.08881387334009 38.0 38.0 38.0 37.0 38.0 66-67 37.074445535818775 38.0 38.0 38.0 37.0 38.0 68-69 37.04247615741563 38.0 38.0 38.0 36.0 38.0 70-71 37.03151244758445 38.0 38.0 38.0 36.0 38.0 72-73 36.769596086305356 38.0 38.0 38.0 35.5 38.0 74-75 36.90943631173333 38.0 38.0 38.0 36.0 38.0 76 36.03850973492753 38.0 37.0 38.0 32.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.07353758613754025 1101 2 -0.01641199177517194 1101 3 -0.010031324716912593 1101 4 -0.017061233231792983 1101 5 0.017248584902297637 1101 6 -4.3560528658304065E-4 1101 7 -4.887215971294268E-4 1101 8 0.04450044447682444 1101 9 -0.025293842519587884 1101 10-11 0.030631602392141133 1101 12-13 0.020786559813970484 1101 14-15 -0.020185468101061588 1101 16-17 -0.004958124143698228 1101 18-19 -0.010670376698939776 1101 20-21 -0.008341115585139391 1101 22-23 -0.03819845858806303 1101 24-25 -0.03896943533334252 1101 26-27 -0.0538003548368593 1101 28-29 -0.05020944371860736 1101 30-31 -0.00683109780100466 1101 32-33 9.169538421147649E-4 1101 34-35 -0.07936578229773517 1101 36-37 -0.04364039295295896 1101 38-39 0.018432062118847625 1101 40-41 -0.06806753021150058 1101 42-43 -0.031217186664392216 1101 44-45 -0.0637421488719383 1101 46-47 -0.018643552036948563 1101 48-49 -0.04327835052338003 1101 50-51 0.006103804887004571 1101 52-53 -0.030920658011169166 1101 54-55 0.03653495843632015 1101 56-57 0.01503731175706946 1101 58-59 0.008273925208108324 1101 60-61 -0.02212250089753809 1101 62-63 0.02307033291619831 1101 64-65 -0.07634023356923336 1101 66-67 -0.018940232760925824 1101 68-69 0.05922898216647354 1101 70-71 -0.030265864504201545 1101 72-73 0.24671669872483193 1101 74-75 0.10600229229574865 1101 76 0.06060513618669461 1102 1 0.03126025943456057 1102 2 0.059708744307847894 1102 3 0.02041950658546199 1102 4 0.0292470071342521 1102 5 0.03249247796634336 1102 6 0.03662728446877139 1102 7 0.08434605024717712 1102 8 0.10811414102246886 1102 9 0.08081395003963365 1102 10-11 0.08783811188859403 1102 12-13 0.04838083250785985 1102 14-15 0.04186939921738286 1102 16-17 0.06304594846479006 1102 18-19 0.020708004255794776 1102 20-21 0.04783021950650834 1102 22-23 0.05974574939917687 1102 24-25 0.010503631682169612 1102 26-27 -0.008957650609012546 1102 28-29 -0.06016544715908623 1102 30-31 0.030677803205314547 1102 32-33 -0.0513518857609796 1102 34-35 0.023735024097199187 1102 36-37 0.0024586655281382264 1102 38-39 0.12400749652748289 1102 40-41 0.11279452099328324 1102 42-43 0.04516234301982536 1102 44-45 0.08764030205556139 1102 46-47 0.07754609793221334 1102 48-49 0.13584740043382482 1102 50-51 0.12072600576487957 1102 52-53 0.11645170013061801 1102 54-55 0.1556448094836611 1102 56-57 0.13584373524158622 1102 58-59 0.13199783059848613 1102 60-61 0.09115191959807589 1102 62-63 0.14364503371938753 1102 64-65 0.17620454272063313 1102 66-67 0.17869593231874603 1102 68-69 0.2420750901918609 1102 70-71 0.11941339726028843 1102 72-73 0.411095607260485 1102 74-75 0.23718140821770817 1102 76 0.4422913239334605 1103 1 -0.07077209746925917 1103 2 -0.09732290635059115 1103 3 -0.054470047502064745 1103 4 -0.07490533542735278 1103 5 -0.08604760417542678 1103 6 -0.05326319317040884 1103 7 0.013589063850552918 1103 8 -0.007633609857627732 1103 9 -0.06718737552862564 1103 10-11 -0.025771127974067554 1103 12-13 -0.049822098953740124 1103 14-15 -0.028211139919477546 1103 16-17 -0.03544382727513096 1103 18-19 -0.009073331308556476 1103 20-21 -0.06143485068341903 1103 22-23 -0.015932816596738064 1103 24-25 -0.05818671774630957 1103 26-27 -0.03916477064900192 1103 28-29 -0.13743591740985295 1103 30-31 -0.050594280544522974 1103 32-33 -0.13262932067775068 1103 34-35 -0.016844178606099547 1103 36-37 -0.06777800816476542 1103 38-39 -0.015246110756621079 1103 40-41 -0.023905167172223685 1103 42-43 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0.012183097470099824 2116 22-23 0.004572168850486946 2116 24-25 -0.09216300935625554 2116 26-27 0.001429134848990543 2116 28-29 -0.07529395513565618 2116 30-31 6.549211588620096E-4 2116 32-33 0.06655947409139884 2116 34-35 -0.0013683394774659519 2116 36-37 -0.03983384952182689 2116 38-39 0.04168614749198696 2116 40-41 0.02717502664739868 2116 42-43 -0.03567046891126324 2116 44-45 -0.06979688223096048 2116 46-47 -0.0761538364064549 2116 48-49 -0.07049079608002273 2116 50-51 -0.1492098418760861 2116 52-53 -0.06240081064207459 2116 54-55 -0.10347354352155946 2116 56-57 -0.08650084712406425 2116 58-59 -0.24468264218078417 2116 60-61 -0.0611660778815164 2116 62-63 -0.11446111405484771 2116 64-65 -0.09094782338502938 2116 66-67 -0.1693307811503928 2116 68-69 -0.047874042159470775 2116 70-71 -0.10689069380305227 2116 72-73 0.17622974903240873 2116 74-75 -0.1294645017794238 2116 76 0.1160047639156474 1117 1 -0.03352460466916085 1117 2 -0.02120561092652906 1117 3 -0.04523020736372274 1117 4 6.781800311586039E-4 1117 5 -0.10490174743815572 1117 6 -0.11812753544320032 1117 7 -0.025961852902923965 1117 8 0.016448581363597725 1117 9 0.02434227172724235 1117 10-11 -0.18008677984604304 1117 12-13 0.0016336088834236762 1117 14-15 0.03298795864598958 1117 16-17 -0.001261075721117777 1117 18-19 -0.1377295538621155 1117 20-21 -0.1514125871567984 1117 22-23 -0.39871037877460935 1117 24-25 -0.2649595957326554 1117 26-27 -0.19652664487028204 1117 28-29 -0.18618704930933205 1117 30-31 -0.20117034078540286 1117 32-33 0.056132704690419644 1117 34-35 0.0517460022935623 1117 36-37 -0.07546076312299732 1117 38-39 -0.011934223939761068 1117 40-41 0.014976073593864214 1117 42-43 0.011712331064146042 1117 44-45 0.04893410635429518 1117 46-47 0.02704730789174903 1117 48-49 0.03550705088504458 1117 50-51 -0.013947498888981613 1117 52-53 0.06571855653842817 1117 54-55 0.08777858916253933 1117 56-57 0.0979294375716151 1117 58-59 0.11086873245560014 1117 60-61 0.05887060345436623 1117 62-63 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30-31 0.021796015205389097 2104 32-33 -0.024198492369166047 2104 34-35 -0.012052334988716495 2104 36-37 -0.17017012731876946 2104 38-39 0.059343802545967606 2104 40-41 -0.07165613069323484 2104 42-43 -0.14578423682963404 2104 44-45 -0.1349354231160902 2104 46-47 -0.027430689631870564 2104 48-49 -0.1667530740267935 2104 50-51 0.02434134170853497 2104 52-53 0.0027817469888020696 2104 54-55 -0.025851834525937534 2104 56-57 -0.20111471727282293 2104 58-59 -0.0316724266197852 2104 60-61 -0.12791473167894196 2104 62-63 -0.16877115520939157 2104 64-65 -0.1965364226612749 2104 66-67 -0.10987033708893534 2104 68-69 -0.317058656820592 2104 70-71 -0.1745457629920537 2104 72-73 0.052401833529849284 2104 74-75 -0.0034900014502952104 2104 76 -0.3406144990291722 2111 1 -0.028129850298682868 2111 2 0.013306441255664936 2111 3 -0.020403219982789267 2111 4 -0.018322779313152182 2111 5 0.010654102022414236 2111 6 -0.0024181525574817897 2111 7 -0.013004858554523935 2111 8 0.10090327414820166 2111 9 0.03416419403855997 2111 10-11 0.09064593494502304 2111 12-13 0.05791118044849952 2111 14-15 0.05874007596009534 2111 16-17 0.013683131842270768 2111 18-19 0.01576754845803663 2111 20-21 0.0719303396196409 2111 22-23 -0.009088835792439909 2111 24-25 0.0017195299510319728 2111 26-27 0.01599275046486781 2111 28-29 0.05050082643784748 2111 30-31 0.029638124829141077 2111 32-33 0.06317377440245053 2111 34-35 0.0469457347122173 2111 36-37 -0.009154066209674738 2111 38-39 0.011861348508944047 2111 40-41 -0.008471029732596946 2111 42-43 0.03249310890932833 2111 44-45 -0.06645488811678035 2111 46-47 0.011884684176102667 2111 48-49 -0.19578664552321356 2111 50-51 0.04061513690231777 2111 52-53 -0.011654143455153587 2111 54-55 -0.05315781138911291 2111 56-57 -0.14885148786500224 2111 58-59 -0.08699837966884161 2111 60-61 -0.13724151236466042 2111 62-63 -0.14482003915401265 2111 64-65 -0.1259717686844013 2111 66-67 -0.07418020635992661 2111 68-69 -0.38708324094925217 2111 70-71 -0.06555220149045482 2111 72-73 -0.004746046303857554 2111 74-75 0.03458336892705205 2111 76 -0.45601379861660263 2110 1 0.03758726992656847 2110 2 -0.011901185936011416 2110 3 0.030689061175358745 2110 4 0.011355021943138865 2110 5 0.049877015831917504 2110 6 0.12797495021107608 2110 7 -0.011884902610248105 2110 8 -0.13071487067026766 2110 9 -0.04265120047924853 2110 10-11 0.03764728310561338 2110 12-13 0.027043923219423505 2110 14-15 0.06533035078832938 2110 16-17 0.022518953696504695 2110 18-19 0.019974528456025098 2110 20-21 -0.057139905425600546 2110 22-23 -0.012854507183341468 2110 24-25 -0.016383036032742382 2110 26-27 0.006171516176770808 2110 28-29 0.00980599999493137 2110 30-31 0.011156715552722574 2110 32-33 -7.697507656914127E-4 2110 34-35 -0.0045502506390775466 2110 36-37 0.008882609180041356 2110 38-39 -0.04980303979430545 2110 40-41 -0.008652901031361182 2110 42-43 -0.056037662697889346 2110 44-45 -0.061665352765899684 2110 46-47 -0.005545274748534723 2110 48-49 0.023845377791673172 2110 50-51 0.020743248253225488 2110 52-53 0.03180403653246344 2110 54-55 -0.06327940218619688 2110 56-57 -0.12041805754837043 2110 58-59 -0.10929381939151028 2110 60-61 -0.13813609964203977 2110 62-63 -0.12326957933416338 2110 64-65 -0.22447157723705402 2110 66-67 -0.10677892402362943 2110 68-69 -0.11107965980099266 2110 70-71 -0.11882999520376814 2110 72-73 0.20787546088694597 2110 74-75 0.0010982404599175766 2110 76 -0.08421833186001493 2109 1 0.18140682488555626 2109 2 0.03871726149549204 2109 3 0.08004274716142135 2109 4 -0.014788353776516772 2109 5 0.05793095027962636 2109 6 -0.05357060549963677 2109 7 0.07839774942717526 2109 8 -0.5342491284195461 2109 9 -0.05894530795814035 2109 10-11 -0.08853643149137014 2109 12-13 -0.19612349527081818 2109 14-15 -0.012260164854176026 2109 16-17 -0.06841245760134029 2109 18-19 -0.1278640735888814 2109 20-21 -0.15183407429708495 2109 22-23 0.010072754269792483 2109 24-25 -0.03133910156658004 2109 26-27 0.01952748316255537 2109 28-29 -0.01872026072187083 2109 30-31 -0.07681856192893122 2109 32-33 -0.12585279687227313 2109 34-35 0.023487422150651582 2109 36-37 -0.005404858301254478 2109 38-39 0.09694158358544769 2109 40-41 -0.10650589773474195 2109 42-43 -0.026561062322521423 2109 44-45 -0.0883441451893816 2109 46-47 0.008348338882839812 2109 48-49 -0.3051472329503184 2109 50-51 -0.09463885918628989 2109 52-53 -0.23351043446691477 2109 54-55 -0.1533272951834448 2109 56-57 -0.28618200791017756 2109 58-59 -0.132538040151438 2109 60-61 -0.2155569090278462 2109 62-63 -0.20432949986826543 2109 64-65 -0.3579834186891091 2109 66-67 -0.12978123455086177 2109 68-69 -0.5266356873181692 2109 70-71 -0.10582228360382118 2109 72-73 0.22558919542114353 2109 74-75 0.0046967512894298125 2109 76 -0.6897402520336371 2108 1 0.1658969670464856 2108 2 0.10200481159433394 2108 3 0.07810059976412731 2108 4 0.05774755296042855 2108 5 0.09771531827541224 2108 6 0.08625101280992453 2108 7 0.15919986063369151 2108 8 -0.09660108924207833 2108 9 0.05438935479158147 2108 10-11 0.1357698897002919 2108 12-13 -0.0647209726449205 2108 14-15 0.14184880630747898 2108 16-17 0.10109983483561535 2108 18-19 0.059789254933235725 2108 20-21 -0.0010707177439428506 2108 22-23 0.21381918458688887 2108 24-25 0.10141057965670086 2108 26-27 0.18041793558727903 2108 28-29 0.13611747384599227 2108 30-31 0.15641175829382092 2108 32-33 0.06071792358144279 2108 34-35 0.16726542860582327 2108 36-37 0.007843825526542503 2108 38-39 0.05029006360271637 2108 40-41 0.0867417248922564 2108 42-43 0.14947182590621821 2108 44-45 0.12989109120850628 2108 46-47 0.18243441509537917 2108 48-49 0.007261994398874094 2108 50-51 0.08083469125840281 2108 52-53 0.020958353735061053 2108 54-55 0.1487193979849195 2108 56-57 1.7848816538190704E-4 2108 58-59 0.1552822317492044 2108 60-61 0.03718422094942753 2108 62-63 0.15005800734940067 2108 64-65 0.00372679052903635 2108 66-67 0.21159785836068323 2108 68-69 -0.008759410003911228 2108 70-71 0.08751488304668698 2108 72-73 0.4292197182882731 2108 74-75 0.18438236930312257 2108 76 -0.017657712934962433 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 0.0 12 5.0 13 78.0 14 203.0 15 392.0 16 631.0 17 989.0 18 1285.0 19 1486.0 20 1591.0 21 1491.0 22 1606.0 23 1704.0 24 1864.0 25 2247.0 26 2570.0 27 3204.0 28 3941.0 29 5224.0 30 6768.0 31 9092.0 32 12754.0 33 18872.0 34 29758.0 35 53114.0 36 126746.0 37 1233402.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.62624768411682 25.619289186584247 13.114440460270687 23.640022669028244 2 14.038426895671188 23.041410423808266 43.01717665405669 19.902986026463857 3 17.75291285178742 26.541369004180094 33.06916814922637 22.63654999480611 4 12.658890295841339 16.35292941963869 42.88693493436633 28.101245350153647 5 11.918399387778448 37.30133371202708 38.70506463434358 12.075202265850898 6 27.369301349490932 41.0965550703542 18.289395654752276 13.244747925402592 7 22.955218149949573 33.45423920032505 26.009093909473783 17.581448740251595 8 20.154199358587473 41.232056425367745 23.61661729184007 14.997126924204709 9 21.88192381681216 17.203017978748445 26.258663605558908 34.65639459888049 10-11 22.63881821253923 27.320814086355323 31.6660618086045 18.374305892500946 12-13 20.64824347903838 25.147434152653027 32.80667947387868 21.397642894429914 14-15 19.660451092623493 28.17596504446364 27.028279744223934 25.13530411868893 16-17 19.036987070501464 33.56054957929492 28.68365134403406 18.718812006169554 18-19 18.439525370508434 32.32913745925426 31.208539280928953 18.022797889308347 20-21 18.8898158996146 30.1019777543724 31.58700291515288 19.42120343086012 22-23 19.013187220664054 30.40480125810477 31.42645254691266 19.155558974318517 24-25 19.05717092105419 30.84986502460852 31.198644591977214 18.894319462360077 26-27 18.85815946951318 31.14424023910302 31.044175677079426 18.953424614304367 28-29 18.64320474839877 31.180433104670684 31.37047030344151 18.805891843489032 30-31 18.831762674734946 30.688065492979042 31.613859928021892 18.86631190426412 32-33 18.853655906767706 30.942171624530413 31.237204293440314 18.966968175261567 34-35 18.694387574637513 30.622780269530015 31.64229483148786 19.040537324344616 36-37 18.753328362977953 30.986056706758237 31.620204363130483 18.64041056713333 38-39 18.733473239632932 30.71002447045334 31.438122362785975 19.11837992712775 40-41 18.878343320065905 30.658184189799197 31.410016186527706 19.053456303607188 42-43 19.08580306084478 30.61729052516144 31.32566478503213 18.971241628961653 44-45 18.54152942305745 30.520020144403286 31.645877958051777 19.292572474487482 46-47 18.613126208894307 30.347668469406674 32.00300719649603 19.03619812520299 48-49 18.43087984494595 30.488692441509567 31.826677922286255 19.253749791258223 50-51 18.377626037298704 30.48902116871727 31.81293712500444 19.32041566897959 52-53 18.237062283286587 30.291719098656294 32.140908260125784 19.33031035793133 54-55 18.21207901550146 30.185342974244882 32.159514220081554 19.443063790172108 56-57 18.06513795365998 30.218643040384798 32.199586066700064 19.516632939255157 58-59 17.796699315852933 30.38070555378043 32.330978331617374 19.49161679874926 60-61 18.049490538573508 30.165816578107556 32.024341592275704 19.760351291043236 62-63 17.905310785276704 29.586763601745673 32.31404888042088 20.19387673255675 64-65 18.070956425236258 29.485154021845894 32.19248555901377 20.251403993904084 66-67 18.270921185679594 29.600570144469035 31.665174245143714 20.463334424707664 68-69 18.146563683007038 29.593502509503505 31.671650171135386 20.588283636354074 70-71 18.37989425503183 29.474306023991826 31.613794182580353 20.532005538395996 72-73 18.32029601227599 29.046829163099975 31.84982031770827 20.783054506915764 74-75 18.705071208887734 29.145052852760458 31.261727343134666 20.888148595217153 76 18.804379698333616 29.055474688662464 31.07175588980538 21.068389723198543 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 0.5 2 0.5 3 1.0 4 1.0 5 3.0 6 6.0 7 11.0 8 15.0 9 28.0 10 64.0 11 148.5 12 210.0 13 329.0 14 651.0 15 1278.0 16 2360.0 17 3018.0 18 3955.5 19 6503.0 20 10001.5 21 11890.0 22 14526.5 23 20237.0 24 26903.5 25 30496.0 26 34396.5 27 42100.5 28 49741.0 29 53578.0 30 57060.5 31 63896.0 32 69320.5 33 71392.0 34 73665.5 35 78089.0 36 82045.5 37 83852.0 38 84772.5 39 86099.0 40 86257.5 41 84393.0 42 82776.0 43 80909.0 44 76206.5 45 70108.5 46 66846.0 47 62153.5 48 53123.0 49 44902.0 50 41019.0 51 37282.5 52 29735.5 53 22887.0 54 19849.0 55 17467.0 56 13182.0 57 9718.5 58 8158.0 59 7231.0 60 5304.0 61 3714.5 62 3125.0 63 2673.5 64 1810.5 65 1172.0 66 819.0 67 693.0 68 548.0 69 349.0 70 228.0 71 161.0 72 132.5 73 88.0 74 64.0 75 56.0 76 41.5 77 26.0 78 24.0 79 23.0 80 22.5 81 18.5 82 15.0 83 15.0 84 13.5 85 12.5 86 11.0 87 9.0 88 9.0 89 7.0 90 7.0 91 7.0 92 5.0 93 5.0 94 6.0 95 9.0 96 11.0 97 8.5 98 7.5 99 59.0 100 109.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 1521018.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.19609611302376 #Duplication Level Percentage of deduplicated Percentage of total 1 96.20529042205962 89.65957492755633 2 2.9913042163787074 5.575557505058464 3 0.3810644499331986 1.0654115730369274 4 0.11124967363322165 0.41472141105857 5 0.07030580152033365 0.3276113117896094 6 0.033889865221213976 0.18950418818482076 7 0.044658140079218506 0.2913375020536209 8 0.0323649128567557 0.2413026823430274 9 0.03188060801994909 0.267403338825193 >10 0.09244929715669434 1.4829103906696597 >50 0.004419077892600523 0.2650918556389888 >100 0.0011235352485023844 0.21957331378477865 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.3149088307962166E-4 0.0 0.0 2 1.3149088307962166E-4 0.0 6.574544153981083E-5 3 1.3149088307962166E-4 0.0 6.574544153981083E-5 4 1.3149088307962166E-4 0.0 6.574544153981083E-5 5 1.3149088307962166E-4 6.574544153981083E-5 6.574544153981083E-5 6 1.3149088307962166E-4 6.574544153981083E-5 6.574544153981083E-5 7 1.3149088307962166E-4 6.574544153981083E-5 1.3149088307962166E-4 8 1.3149088307962166E-4 6.574544153981083E-5 1.972363246194325E-4 9 1.3149088307962166E-4 6.574544153981083E-5 1.972363246194325E-4 10 1.972363246194325E-4 1.3149088307962166E-4 2.6298176615924333E-4 11 2.6298176615924333E-4 1.3149088307962166E-4 2.6298176615924333E-4 12 2.6298176615924333E-4 1.972363246194325E-4 3.287272076990542E-4 13 3.287272076990542E-4 1.972363246194325E-4 3.287272076990542E-4 14 3.287272076990542E-4 1.972363246194325E-4 3.287272076990542E-4 15 3.287272076990542E-4 1.972363246194325E-4 3.287272076990542E-4 16 3.287272076990542E-4 1.972363246194325E-4 3.287272076990542E-4 17 3.287272076990542E-4 1.972363246194325E-4 3.287272076990542E-4 18 3.287272076990542E-4 2.6298176615924333E-4 3.94472649238865E-4 19 3.94472649238865E-4 2.6298176615924333E-4 4.6021809077867584E-4 20 5.259635323184867E-4 2.6298176615924333E-4 5.259635323184867E-4 21 5.917089738582975E-4 2.6298176615924333E-4 7.8894529847773E-4 22 6.574544153981084E-4 2.6298176615924333E-4 0.0013149088307962168 23 6.574544153981084E-4 2.6298176615924333E-4 0.0015121451554156492 24 6.574544153981084E-4 2.6298176615924333E-4 0.0019066178046545142 25 7.231998569379192E-4 2.6298176615924333E-4 0.0021038541292739466 26 7.231998569379192E-4 2.6298176615924333E-4 0.002432581336973001 27 7.8894529847773E-4 3.287272076990542E-4 0.007100507686299571 28 7.8894529847773E-4 3.287272076990542E-4 0.016304869501873088 29 7.8894529847773E-4 3.287272076990542E-4 0.024128577045110577 30 7.8894529847773E-4 3.287272076990542E-4 0.029453957809835254 31 7.8894529847773E-4 3.287272076990542E-4 0.03385890239300258 32 8.546907400175409E-4 3.287272076990542E-4 0.04161686449470026 33 8.546907400175409E-4 3.287272076990542E-4 0.05325380764724678 34 8.546907400175409E-4 3.287272076990542E-4 0.07468682158922511 35 9.204361815573517E-4 3.287272076990542E-4 0.1134108866561737 36 9.204361815573517E-4 3.287272076990542E-4 0.18060272790986037 37 9.204361815573517E-4 3.287272076990542E-4 0.2971036503184052 38 9.204361815573517E-4 3.287272076990542E-4 0.44056020375827243 39 9.204361815573517E-4 3.287272076990542E-4 0.6152458419295498 40 9.861816230971627E-4 3.287272076990542E-4 0.8103783124197084 41 9.861816230971627E-4 3.94472649238865E-4 1.0064312190914242 42 9.861816230971627E-4 3.94472649238865E-4 1.2200381586542697 43 9.861816230971627E-4 3.94472649238865E-4 1.4620471289623134 44 0.0011176725061767842 3.94472649238865E-4 1.7388354378449171 45 0.0013149088307962168 4.6021809077867584E-4 2.0786078797226595 46 0.0013149088307962168 4.6021809077867584E-4 2.4497408972148915 47 0.0013149088307962168 5.259635323184867E-4 2.834088748456626 48 0.0013806542723360277 5.259635323184867E-4 3.2185023451398997 49 0.0013806542723360277 5.259635323184867E-4 3.606203213900164 50 0.0013806542723360277 5.259635323184867E-4 4.013759206005451 51 0.0013806542723360277 5.259635323184867E-4 4.407377164504299 52 0.0014463997138758383 5.259635323184867E-4 4.826372863437514 53 0.0015121451554156492 5.259635323184867E-4 5.247406671058462 54 0.0015121451554156492 5.259635323184867E-4 5.70716454374636 55 0.0015121451554156492 5.259635323184867E-4 6.20373986369655 56 0.00157789059695546 5.917089738582975E-4 6.7081388911899795 57 0.00157789059695546 6.574544153981084E-4 7.230749405989935 58 0.00157789059695546 6.574544153981084E-4 7.774201225758012 59 0.001643636038495271 6.574544153981084E-4 8.295496831727172 60 0.0017093814800350818 6.574544153981084E-4 8.823169745525695 61 0.0017093814800350818 6.574544153981084E-4 9.329146663616077 62 0.0017751269215748927 6.574544153981084E-4 9.829600964617118 63 0.0017751269215748927 6.574544153981084E-4 10.332553592396671 64 0.0017751269215748927 6.574544153981084E-4 10.86127843325983 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACACTG 540 0.0 20.740742 5 TGTGCGG 325 0.0 20.461538 10 ACGGGGC 105 5.4676767E-4 20.0 8 GCTATAC 185 2.2731729E-7 18.918919 3 CCTATAC 315 0.0 18.88889 3 AGGGTGC 130 1.05792235E-4 18.846155 8 CTATACG 115 0.0010082219 18.260868 4 GCTAGAC 230 6.0827006E-9 18.260868 3 ACTGTGC 490 0.0 17.857143 8 GTACTAG 260 1.4624675E-9 17.500002 1 CGCTCGG 100 0.00969177 17.5 10 TACAGCG 180 3.6600031E-6 17.499998 5 ACTGTCC 360 1.8189894E-12 16.527777 8 TATACTC 425 0.0 16.470589 5 GTGTGCG 490 0.0 16.428572 9 CATACAC 645 0.0 16.27907 3 AGGCTGC 215 1.0979529E-6 16.27907 6 GGGGTGT 195 7.838957E-6 16.153847 6 GTGTAGG 390 0.0 16.153847 1 CTAGGAG 240 1.9615618E-7 16.041668 3 >>END_MODULE