FastQCFastQC Report
Fri 12 Jun 2015
000000000-AFM8U l01n02 ve18.3420000000c8a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFM8U l01n02 ve18.3420000000c8a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1521018
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG5400.020.7407425
TGTGCGG3250.020.46153810
ACGGGGC1055.4676767E-420.08
GCTATAC1852.2731729E-718.9189193
CCTATAC3150.018.888893
AGGGTGC1301.05792235E-418.8461558
CTATACG1150.001008221918.2608684
GCTAGAC2306.0827006E-918.2608683
ACTGTGC4900.017.8571438
GTACTAG2601.4624675E-917.5000021
CGCTCGG1000.0096917717.510
TACAGCG1803.6600031E-617.4999985
ACTGTCC3601.8189894E-1216.5277778
TATACTC4250.016.4705895
GTGTGCG4900.016.4285729
CATACAC6450.016.279073
AGGCTGC2151.0979529E-616.279076
GGGGTGT1957.838957E-616.1538476
GTGTAGG3900.016.1538471
CTAGGAG2401.9615618E-716.0416683
Produced by FastQC (version 0.11.2)