##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AFM8U l01n02 ve17.3420000000c886.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1143958 Sequences flagged as poor quality 0 Sequence length 76 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.20724799337039 34.0 34.0 34.0 34.0 34.0 2 33.43777393925301 34.0 34.0 34.0 34.0 34.0 3 33.507532619204554 34.0 34.0 34.0 34.0 34.0 4 33.513426192220344 34.0 34.0 34.0 34.0 34.0 5 33.490795990761896 34.0 34.0 34.0 34.0 34.0 6 37.21455245734546 38.0 38.0 38.0 37.0 38.0 7 37.187009488110576 38.0 38.0 38.0 37.0 38.0 8 37.13740801672789 38.0 38.0 38.0 37.0 38.0 9 37.18662223613104 38.0 38.0 38.0 37.0 38.0 10-11 37.161210901099516 38.0 38.0 38.0 37.0 38.0 12-13 37.18194767640071 38.0 38.0 38.0 37.0 38.0 14-15 37.15207507618287 38.0 38.0 38.0 37.0 38.0 16-17 37.15714825194631 38.0 38.0 38.0 37.0 38.0 18-19 37.13475888100787 38.0 38.0 38.0 37.0 38.0 20-21 37.12841205708601 38.0 38.0 38.0 37.0 38.0 22-23 37.087867299323925 38.0 38.0 38.0 37.0 38.0 24-25 37.116319829923825 38.0 38.0 38.0 37.0 38.0 26-27 37.099358542883564 38.0 38.0 38.0 37.0 38.0 28-29 37.14252883410055 38.0 38.0 38.0 37.0 38.0 30-31 37.091106491671894 38.0 38.0 38.0 37.0 38.0 32-33 37.07883069133657 38.0 38.0 38.0 36.5 38.0 34-35 37.07194975689667 38.0 38.0 38.0 37.0 38.0 36-37 37.01988840499389 38.0 38.0 38.0 36.5 38.0 38-39 37.00113902783144 38.0 38.0 38.0 36.0 38.0 40-41 36.99324538138638 38.0 38.0 38.0 36.0 38.0 42-43 36.98143244769476 38.0 38.0 38.0 36.0 38.0 44-45 36.997037915727674 38.0 38.0 38.0 36.0 38.0 46-47 36.943071773614065 38.0 38.0 38.0 36.0 38.0 48-49 36.92377080277423 38.0 38.0 38.0 36.0 38.0 50-51 36.90420758454419 38.0 38.0 38.0 36.0 38.0 52-53 36.88561118502602 38.0 38.0 38.0 36.0 38.0 54-55 36.860648730110725 38.0 38.0 38.0 36.0 38.0 56-57 36.84523426559367 38.0 38.0 38.0 36.0 38.0 58-59 36.81646572688858 38.0 38.0 38.0 36.0 38.0 60-61 36.817199582502155 38.0 38.0 38.0 36.0 38.0 62-63 36.740390818544036 38.0 38.0 38.0 35.0 38.0 64-65 36.707261979897865 38.0 38.0 38.0 35.0 38.0 66-67 36.69431657455955 38.0 38.0 38.0 35.0 38.0 68-69 36.62948333767498 38.0 38.0 38.0 35.0 38.0 70-71 36.62043361731811 38.0 38.0 38.0 35.0 38.0 72-73 36.34956484416386 38.0 37.5 38.0 34.0 38.0 74-75 36.480922376520816 38.0 38.0 38.0 34.0 38.0 76 35.42886364709194 38.0 36.0 38.0 31.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.035568687473535476 1101 2 0.0559447680859364 1101 3 0.08326686695964725 1101 4 -0.03466287661735379 1101 5 0.026627651848947664 1101 6 0.06443298587895185 1101 7 -0.03196095774182339 1101 8 0.13049480887649878 1101 9 0.07332213046276337 1101 10-11 0.13733928907604565 1101 12-13 0.08493740820085094 1101 14-15 4.381229937422404E-5 1101 16-17 0.04205998871913863 1101 18-19 -0.03652812924998727 1101 20-21 0.028683533567374297 1101 22-23 0.10389434157789879 1101 24-25 0.01723973468191531 1101 26-27 0.05627381985912905 1101 28-29 0.05469245773240772 1101 30-31 0.03715989912045359 1101 32-33 0.07996381251943774 1101 34-35 0.10569985599484966 1101 36-37 0.055962117427355906 1101 38-39 0.06150381034979091 1101 40-41 -0.0032612399828337857 1101 42-43 0.02869389415151602 1101 44-45 0.09380990597907157 1101 46-47 0.05677206829381731 1101 48-49 0.07073805398984234 1101 50-51 0.15338324027530348 1101 52-53 0.02768152278216718 1101 54-55 0.1441351324646689 1101 56-57 0.017856584344919213 1101 58-59 0.04653589899483279 1101 60-61 -0.01924859442500093 1101 62-63 0.13057970452719303 1101 64-65 -0.0038823036854864768 1101 66-67 0.0043333448355582505 1101 68-69 0.1838500799316023 1101 70-71 -0.013937434008084892 1101 72-73 0.3365655582849243 1101 74-75 0.18242947732116477 1101 76 0.04757923334472025 1102 1 0.026155904325648294 1102 2 0.01631726059945038 1102 3 0.061917700028082834 1102 4 0.012027030278318307 1102 5 0.04461192156536953 1102 6 0.09238485767743043 1102 7 0.025214412488772098 1102 8 0.2098310692728802 1102 9 0.12484311162343431 1102 10-11 0.1570738337528823 1102 12-13 0.07552750686913612 1102 14-15 0.0801816663592021 1102 16-17 0.05952368204000891 1102 18-19 0.06887381420737881 1102 20-21 0.09992355176300549 1102 22-23 0.08075636554550414 1102 24-25 0.032006455888137 1102 26-27 0.047954466029665355 1102 28-29 -0.09748272661533974 1102 30-31 -0.0067904810204098 1102 32-33 -0.12596853406142827 1102 34-35 -0.020806369409612557 1102 36-37 0.00498207623238045 1102 38-39 0.0936263501694441 1102 40-41 0.10410557913562002 1102 42-43 0.15129464787511893 1102 44-45 0.1206391707058998 1102 46-47 0.19275173981167626 1102 48-49 0.18764301271758654 1102 50-51 0.2003695676521744 1102 52-53 0.10305226507293952 1102 54-55 0.23000292966105462 1102 56-57 0.15056826835577652 1102 58-59 0.15870523116581126 1102 60-61 0.1422933622571918 1102 62-63 0.19779431483529208 1102 64-65 0.15701527653758518 1102 66-67 0.10313597735436275 1102 68-69 0.23124237335414932 1102 70-71 0.1899763084151047 1102 72-73 0.4796661614225428 1102 74-75 0.2318567179717803 1102 76 0.5470526000924067 1103 1 -0.04293568871243991 1103 2 0.0470886279016014 1103 3 -2.239053961190507E-4 1103 4 0.050325778404761934 1103 5 0.013859321106458822 1103 6 0.09846677453113983 1103 7 0.03741807360795946 1103 8 0.1452247517695895 1103 9 0.11384344775844824 1103 10-11 0.14120156756064262 1103 12-13 0.05192496942511582 1103 14-15 0.08247893649248539 1103 16-17 0.08783763547988599 1103 18-19 0.09793260280347482 1103 20-21 0.0600264941538029 1103 22-23 0.06957539312275429 1103 24-25 0.027504498714904457 1103 26-27 0.08261922348281558 1103 28-29 -9.085512929871697E-4 1103 30-31 0.07226453335498917 1103 32-33 -0.07066713021814053 1103 34-35 0.09801360485062105 1103 36-37 0.05245801922455229 1103 38-39 0.10669307755727431 1103 40-41 -0.009056921708889831 1103 42-43 0.11619178889251458 1103 44-45 0.12154811148024436 1103 46-47 0.1349638334990928 1103 48-49 0.1494066122148041 1103 50-51 0.13072633368301467 1103 52-53 0.11470720454777705 1103 54-55 0.08916955253841508 1103 56-57 0.05352888384151555 1103 58-59 0.10268432740468114 1103 60-61 0.08746071245398923 1103 62-63 0.05826262681196681 1103 64-65 0.10927201028945888 1103 66-67 0.047048451299723126 1103 68-69 0.192219850640015 1103 70-71 0.11452765574933466 1103 72-73 0.42393916727863257 1103 74-75 0.15508291765266335 1103 76 0.43914220884960287 2116 1 0.005857806411675881 2116 2 0.13418654356085824 2116 3 0.11742763494076058 2116 4 0.007598178267748779 2116 5 0.08783755087757328 2116 6 0.06122202819813083 2116 7 0.13858957082749157 2116 8 0.12155554641820032 2116 9 0.09269529737159843 2116 10-11 0.032227329669765936 2116 12-13 -0.058121535350032616 2116 14-15 0.0036021533686962925 2116 16-17 0.02724511200713664 2116 18-19 0.06373685963374953 2116 20-21 0.08463102097117314 2116 22-23 0.05271819917530962 2116 24-25 -0.07755404466436033 2116 26-27 0.019289050912703942 2116 28-29 -0.09085713537180595 2116 30-31 0.02398513447926831 2116 32-33 0.04066886520819679 2116 34-35 0.13880980236432094 2116 36-37 0.0030156861531409618 2116 38-39 0.0597507067078169 2116 40-41 -0.006877443247411463 2116 42-43 -0.09487132311183188 2116 44-45 -4.998490089533902E-4 2116 46-47 -0.07140315251798768 2116 48-49 -7.623815795199107E-4 2116 50-51 -0.13945249499606405 2116 52-53 -0.09968446816336751 2116 54-55 -0.125390862113413 2116 56-57 -0.14922589130355135 2116 58-59 -0.3258150530353561 2116 60-61 -0.1078102316949412 2116 62-63 -0.24030640375299583 2116 64-65 -0.1839029448805931 2116 66-67 -0.13531877145977944 2116 68-69 -0.14144781840415277 2116 70-71 -0.041116321422649094 2116 72-73 0.2404833071480681 2116 74-75 -0.20489306810249985 2116 76 0.2594471532403162 1117 1 -0.01138018168165189 1117 2 0.08276281273714403 1117 3 0.06545058134069848 1117 4 0.05355591526529224 1117 5 -6.176721553501352E-4 1117 6 -0.06160748491123513 1117 7 0.05966757722849536 1117 8 0.1367512514641689 1117 9 0.09305527037780337 1117 10-11 -0.20931904671333257 1117 12-13 0.10806144368968518 1117 14-15 0.12871532353653237 1117 16-17 0.1319604034265751 1117 18-19 -0.07010471632974458 1117 20-21 -0.07819631643127423 1117 22-23 -0.340307386469874 1117 24-25 -0.19131271825987994 1117 26-27 -0.10962147950877466 1117 28-29 -0.15412479242360178 1117 30-31 -0.18350808783872452 1117 32-33 0.20867446859310945 1117 34-35 0.16951013360630185 1117 36-37 0.06604781562315765 1117 38-39 0.07838766182060652 1117 40-41 0.17300288452011614 1117 42-43 0.16344616222197317 1117 44-45 0.1486798343150184 1117 46-47 0.1639605204427781 1117 48-49 0.15512722781218002 1117 50-51 0.1008870113314515 1117 52-53 0.1570610584829808 1117 54-55 0.238582354558595 1117 56-57 0.18631488670585128 1117 58-59 0.23939163494571858 1117 60-61 0.26979936379896685 1117 62-63 0.06188957774556059 1117 64-65 0.21495017360481228 1117 66-67 0.155972053321058 1117 68-69 0.24095229952668262 1117 70-71 0.24067917131441163 1117 72-73 0.5143841366728665 1117 74-75 0.38657254403536 1117 76 0.552402539973265 2117 1 0.20350782979326 2117 2 0.13251276923045197 2117 3 0.11799247818246528 2117 4 0.10680748244526228 2117 5 0.038336781657235974 2117 6 0.11243723537932482 2117 7 0.1387016076009715 2117 8 0.2736358299792556 2117 9 0.18392499944273766 2117 10-11 0.14254045233160184 2117 12-13 0.18511993630063728 2117 14-15 0.14289245441509735 2117 16-17 0.2283285647898765 2117 18-19 0.2122854433533803 2117 20-21 0.20469557872031174 2117 22-23 0.12110447147252756 2117 24-25 0.26562295818887094 2117 26-27 0.17536040626538352 2117 28-29 0.22043153319927455 2117 30-31 0.24170933086427482 2117 32-33 0.3332113883525807 2117 34-35 0.25627781787749626 2117 36-37 0.16289142063997275 2117 38-39 0.20853871873070062 2117 40-41 0.22360920298578435 2117 42-43 0.2838581883485318 2117 44-45 0.17240391966242896 2117 46-47 0.24765237536370677 2117 48-49 0.31922946875120317 2117 50-51 0.09736369946419643 2117 52-53 0.2373149456026269 2117 54-55 0.20220762858816244 2117 56-57 0.29013531694844374 2117 58-59 0.27113148953947075 2117 60-61 0.385059654853535 2117 62-63 0.3504583139397468 2117 64-65 0.41709312345464156 2117 66-67 0.3298341915634211 2117 68-69 0.3104180915801322 2117 70-71 0.24169234518301863 2117 72-73 0.6249352715402878 2117 74-75 0.48604480888324275 2117 76 0.7154092886230998 1116 1 0.10547724906631828 1116 2 0.04355416061412143 1116 3 0.07483138816240853 1116 4 0.07799457538974508 1116 5 -0.08086278202620178 1116 6 0.04123773373936501 1116 7 0.12135710197010496 1116 8 0.1282941632566903 1116 9 0.09979634977710106 1116 10-11 0.09203423205379124 1116 12-13 0.15244178348588378 1116 14-15 0.09714408850614831 1116 16-17 0.09718343084304593 1116 18-19 0.14756854187172053 1116 20-21 0.16074416798578284 1116 22-23 0.05803727670966907 1116 24-25 0.15660114487601362 1116 26-27 0.11971222799603254 1116 28-29 0.12835094291428106 1116 30-31 0.08392291637360216 1116 32-33 0.2724380205990826 1116 34-35 0.16669217188751873 1116 36-37 0.06062783982581976 1116 38-39 0.1517254436064519 1116 40-41 0.07310168314863574 1116 42-43 0.19312232755397218 1116 44-45 0.07524079478400125 1116 46-47 0.1478066037105279 1116 48-49 0.1794821953105128 1116 50-51 0.09429998835874898 1116 52-53 0.18687454171635665 1116 54-55 0.09403702663228586 1116 56-57 0.26638031574111665 1116 58-59 0.15685851333062573 1116 60-61 0.3033729190782566 1116 62-63 0.30388556628566477 1116 64-65 0.2815901688436284 1116 66-67 0.25404441582544024 1116 68-69 0.279806342893103 1116 70-71 0.1670542514940152 1116 72-73 0.46161262952779936 1116 74-75 0.31059937444475594 1116 76 0.4836873809035467 2118 1 0.03920859142371569 2118 2 0.07074467541612961 2118 3 0.03326424960769714 2118 4 0.0781734984590301 2118 5 -0.1311208242561861 2118 6 0.03838551385222644 2118 7 -0.004619218130507363 2118 8 0.0554682336841168 2118 9 0.060259419228209765 2118 10-11 -0.06794776187317098 2118 12-13 0.0759305040928524 2118 14-15 -0.12670754600262768 2118 16-17 0.08408507279833088 2118 18-19 0.015333792736342389 2118 20-21 -0.10142461748856135 2118 22-23 -0.13043842284850626 2118 24-25 0.03878987044733151 2118 26-27 0.05056094388688592 2118 28-29 0.046343312310781926 2118 30-31 0.06294496217790169 2118 32-33 0.03542902480222665 2118 34-35 -0.059479567368825315 2118 36-37 0.025197762547598757 2118 38-39 0.06064910240739607 2118 40-41 -0.01607714270233629 2118 42-43 0.013832414264427939 2118 44-45 0.05395696487882162 2118 46-47 -0.006732560791128606 2118 48-49 0.1237420743461044 2118 50-51 -0.0706983672037893 2118 52-53 0.043615132224537945 2118 54-55 -0.04752188973514393 2118 56-57 0.0537666013395679 2118 58-59 0.1266067592639999 2118 60-61 0.010653226370870073 2118 62-63 0.17164574938705357 2118 64-65 0.12064959198738734 2118 66-67 0.0506922972935584 2118 68-69 0.08062918216433701 2118 70-71 0.03090858536238983 2118 72-73 0.380070716023134 2118 74-75 0.22802032768067448 2118 76 0.4888190600086446 1119 1 0.13477137506188797 1119 2 0.125296929170986 1119 3 0.034304633250471284 1119 4 0.03188814191633327 1119 5 -0.048119423096032676 1119 6 0.06508213888157854 1119 7 0.06592205647021387 1119 8 -0.0010388450773959335 1119 9 0.03138537353647308 1119 10-11 0.0125454109360561 1119 12-13 0.12203929039736039 1119 14-15 -0.013021365081947067 1119 16-17 0.06066127336872995 1119 18-19 0.09731212208690465 1119 20-21 0.1104154211727888 1119 22-23 0.10613002662316973 1119 24-25 0.07921064736683547 1119 26-27 0.04726395494292035 1119 28-29 0.09866647141176088 1119 30-31 0.07736530080990889 1119 32-33 0.11360351029820492 1119 34-35 0.024800346668243378 1119 36-37 0.12066410201538957 1119 38-39 0.1546312790644535 1119 40-41 0.16939367901350266 1119 42-43 0.29472731574649913 1119 44-45 0.23540458978421697 1119 46-47 0.30464277805793216 1119 48-49 0.17104197753582184 1119 50-51 0.23666041713940444 1119 52-53 0.11140895090635894 1119 54-55 0.0550240480771933 1119 56-57 0.20629886228067562 1119 58-59 0.30207008615900577 1119 60-61 0.13025655003363568 1119 62-63 0.30302493763467453 1119 64-65 0.1895272046489751 1119 66-67 0.24434225411858534 1119 68-69 0.3248103402998268 1119 70-71 0.18101441767192483 1119 72-73 0.44259865162102585 1119 74-75 0.39345670355561424 1119 76 0.24806187912822963 2119 1 0.15420015416808752 2119 2 0.04236462849675604 2119 3 -0.0028698410207752545 2119 4 -0.02757309577071254 2119 5 -0.004780638224985978 2119 6 0.06809802980069435 2119 7 -0.00683811346738139 2119 8 0.15420779000794482 2119 9 0.07684994480749907 2119 10-11 0.003528912201815615 2119 12-13 0.025013981476888603 2119 14-15 -0.07867949727233992 2119 16-17 0.12344152626995708 2119 18-19 0.08739721620425911 2119 20-21 0.09885628138783886 2119 22-23 0.15953410462402218 2119 24-25 0.11098010998307473 2119 26-27 0.062340433124234096 2119 28-29 0.06142902248075899 2119 30-31 0.10758138848567711 2119 32-33 0.19166796376588735 2119 34-35 0.13282272303739973 2119 36-37 0.16583369496724032 2119 38-39 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2104 50-51 -0.11739181861513259 2104 52-53 -0.17777566492107155 2104 54-55 -0.17158145620759768 2104 56-57 -0.47309870682389743 2104 58-59 -0.09308565633104138 2104 60-61 -0.23891193643196118 2104 62-63 -0.2649420530913318 2104 64-65 -0.24964180034670846 2104 66-67 -0.2977095474768632 2104 68-69 -0.593368852274125 2104 70-71 -0.32980599800025345 2104 72-73 -0.14865456969223345 2104 74-75 -0.019957395233589637 2104 76 -0.4247278369260101 2111 1 -0.12881910059325463 2111 2 -0.0386087533442705 2111 3 -0.09930112324654772 2111 4 -0.14907591811565624 2111 5 -0.06544698645087976 2111 6 -0.11484597696944121 2111 7 -0.15722624042491873 2111 8 0.12209870877501316 2111 9 -0.035840574381829526 2111 10-11 0.01978471393099568 2111 12-13 -6.750829179367202E-4 2111 14-15 -0.03131197735888236 2111 16-17 0.029480395674340798 2111 18-19 0.06834726026249882 2111 20-21 -0.0039933273226040455 2111 22-23 -0.1830219060546483 2111 24-25 -0.08915190116671567 2111 26-27 -0.07113712552557416 2111 28-29 -0.14085404519444467 2111 30-31 -0.018766804690095285 2111 32-33 -0.0917180431612934 2111 34-35 -0.1043610718169532 2111 36-37 -0.1955918400568848 2111 38-39 -0.09681418125718722 2111 40-41 -0.16902807599169023 2111 42-43 -0.032525444322992314 2111 44-45 -0.18607701660776854 2111 46-47 -0.0575889521451316 2111 48-49 -0.20773891661127664 2111 50-51 -0.0230704901510137 2111 52-53 -0.03418843599539656 2111 54-55 0.03579965584903988 2111 56-57 -0.26615598728291445 2111 58-59 -0.11295665891745443 2111 60-61 -0.32342951652822194 2111 62-63 -0.24378185644737016 2111 64-65 -0.13387480100725213 2111 66-67 -0.22192437001938714 2111 68-69 -0.5291243018664957 2111 70-71 -0.27581534700888 2111 72-73 -0.1928460610114442 2111 74-75 -0.07813626350980485 2111 76 -0.5244030261007282 2110 1 0.04949550339237163 2110 2 0.04024247664791858 2110 3 0.019927194536762727 2110 4 -0.08235111299320863 2110 5 0.025824886815968284 2110 6 0.054362735940699736 2110 7 -2.792626268401932E-4 2110 8 -0.21714359804142447 2110 9 -0.08100048400295634 2110 10-11 -0.014811987730730891 2110 12-13 -0.12713779070605824 2110 14-15 -0.006026457054382206 2110 16-17 -0.07412615701476 2110 18-19 -0.037315450129526084 2110 20-21 -0.03321580607371999 2110 22-23 -0.10654267391004879 2110 24-25 -0.10783854787085545 2110 26-27 -0.01909243548193018 2110 28-29 -0.07116090069252579 2110 30-31 -0.017133084633734086 2110 32-33 -0.08462646126017148 2110 34-35 -0.07258728589798125 2110 36-37 -0.06769000787017632 2110 38-39 -0.17133583223278492 2110 40-41 -0.13931899185021734 2110 42-43 -0.13332454197572474 2110 44-45 -0.16361853877592125 2110 46-47 0.006813375888519602 2110 48-49 -0.06302193879111684 2110 50-51 -0.015875856248342757 2110 52-53 -0.036693128552279575 2110 54-55 -0.08558479112207351 2110 56-57 -0.1609223986774353 2110 58-59 -0.04258690508096663 2110 60-61 -0.09626163778479224 2110 62-63 -0.232543782842086 2110 64-65 -0.2938239457453875 2110 66-67 -0.1849652026749098 2110 68-69 -0.17837847390323702 2110 70-71 -0.19089544102391898 2110 72-73 0.12060389332133781 2110 74-75 -0.15112468902053422 2110 76 -0.21287990589303973 2109 1 0.18577066698859568 2109 2 0.012488254870376636 2109 3 0.07659444469350518 2109 4 0.02560913866152248 2109 5 -0.0030864990308501206 2109 6 -0.06269409474143117 2109 7 0.06353734440808267 2109 8 -0.7478068620067049 2109 9 -0.04576910281154767 2109 10-11 -0.09524627094836546 2109 12-13 -0.3685414214535143 2109 14-15 -0.06568728115511391 2109 16-17 -0.15285197189854216 2109 18-19 -0.23505012811808967 2109 20-21 -0.38541569663413355 2109 22-23 -0.009456279991361782 2109 24-25 -0.10333994496198073 2109 26-27 0.00724656865537554 2109 28-29 0.029984425411157645 2109 30-31 -0.11483161517733009 2109 32-33 -0.2771736113256864 2109 34-35 0.022142777982452344 2109 36-37 0.013612998996549663 2109 38-39 0.032296064995364304 2109 40-41 -0.13935547953404637 2109 42-43 -0.1632646810559777 2109 44-45 -0.158980203290092 2109 46-47 -0.05187547967000938 2109 48-49 -0.5127458408286145 2109 50-51 -0.14695199419477945 2109 52-53 -0.3673341362036595 2109 54-55 -0.2905765878460187 2109 56-57 -0.39466144533286496 2109 58-59 -0.2511563133375603 2109 60-61 -0.4345830682334011 2109 62-63 -0.41814272758541904 2109 64-65 -0.5448071773668559 2109 66-67 -0.20774894545245814 2109 68-69 -0.7578161505927952 2109 70-71 -0.1531723968576344 2109 72-73 0.2266003801792067 2109 74-75 -0.07884317398620055 2109 76 -1.0771628513384357 2108 1 0.19282014840605655 2108 2 0.025780790985947988 2108 3 0.009468101935141249 2108 4 0.07340824966288295 2108 5 0.07292211314349117 2108 6 0.09009097131610844 2108 7 0.11170301300914076 2108 8 -0.4235345753046431 2108 9 -0.02398014738495391 2108 10-11 0.012921018223138958 2108 12-13 -0.09626050714309997 2108 14-15 0.12053362574696536 2108 16-17 0.011051364933166496 2108 18-19 -0.02105765991213815 2108 20-21 -0.08664231411777479 2108 22-23 0.13432883236819038 2108 24-25 0.16534484637028157 2108 26-27 0.09636250889491293 2108 28-29 0.10649078422089531 2108 30-31 0.09040784339780572 2108 32-33 0.04615334261924886 2108 34-35 0.16625779218173875 2108 36-37 -0.022896137933933858 2108 38-39 -0.03467593772101907 2108 40-41 0.05973545761868593 2108 42-43 0.09525296001082495 2108 44-45 0.07316844160433789 2108 46-47 0.06571426906440792 2108 48-49 -0.32672943448214653 2108 50-51 -0.07834784553445218 2108 52-53 -0.13492118398734476 2108 54-55 -0.027683406589069648 2108 56-57 -0.23746032482415558 2108 58-59 0.021435210307345187 2108 60-61 -0.14843084265000073 2108 62-63 0.0705422180868922 2108 64-65 -0.1067380484620557 2108 66-67 0.060403372734974425 2108 68-69 -0.24310175684047408 2108 70-71 -0.17090220918014154 2108 72-73 0.3008070723634262 2108 74-75 0.01100588145274628 2108 76 -0.19852042634769873 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 7.0 13 43.0 14 159.0 15 397.0 16 700.0 17 988.0 18 1266.0 19 1413.0 20 1527.0 21 1654.0 22 1862.0 23 2052.0 24 2369.0 25 2864.0 26 3475.0 27 4247.0 28 5300.0 29 6808.0 30 8567.0 31 11175.0 32 14860.0 33 20749.0 34 30393.0 35 49694.0 36 108052.0 37 863336.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.411499373228736 24.919883422293477 11.458287804272535 24.210329400205254 2 15.8711246391913 22.904075149612137 39.718416235561094 21.50638397563547 3 18.316231889632313 25.86126413731973 31.728175335108453 24.094328637939505 4 13.442364142739507 16.524994798760094 41.144867206663186 28.887773851837217 5 13.227321282774367 35.38879923913291 37.32925509502971 14.054624383063013 6 27.38465922699959 38.882721218786 19.22535617566379 14.507263378550611 7 24.36776525012282 32.68485381456312 24.659122100636562 18.2882588346775 8 21.480858562989198 38.50001486068545 23.20723313268494 16.811893443640415 9 22.032976735159856 17.560784574258843 25.19996363502856 35.20627505555274 10-11 23.246045746434746 26.295764354985064 30.941520580301024 19.516669318279167 12-13 22.159685932525495 24.872984847345794 30.49801653557211 22.4693126845566 14-15 20.992553922434215 27.644196727502234 25.612915858799013 25.750333491264538 16-17 20.459404978154794 32.03373725259144 27.72868409504545 19.77817367420832 18-19 19.62213647703849 31.11997992933307 30.166885497544488 19.090998096083947 20-21 20.059783663386245 28.686061900873984 30.283847833574313 20.970306602165465 22-23 19.873456892648157 29.419349311775434 30.482150568464927 20.225043227111485 24-25 20.376272555460954 30.15150031731934 29.68229602835069 19.789931098869015 26-27 19.87835217726525 29.86818572010511 29.923345087844133 20.330117014785507 28-29 19.645126831579482 30.15058245145364 30.36090485839515 19.84338585857173 30-31 19.90103657651767 29.69938581661215 30.49784170397864 19.90173590289154 32-33 19.834032368321214 29.978985242465196 30.11478568269115 20.07219670652244 34-35 19.569686998998215 29.59562326588913 30.91380977273641 19.920879962376244 36-37 19.855449238520993 30.194071810328698 30.511216320878916 19.439262630271394 38-39 19.73009498600473 29.451430909176736 30.251198033494237 20.567276071324297 40-41 19.727122848915783 29.53958974018277 30.75978313889146 19.973504272009986 42-43 20.414822921820555 29.840649744133962 30.062379912549236 19.682147421496243 44-45 19.554520358264902 29.424244596392523 30.591901101264206 20.42933394407837 46-47 19.41885104173405 29.509606121903076 31.22771989880747 19.8438229375554 48-49 19.421866886721364 29.429271004704717 30.930156526725632 20.218705581848283 50-51 19.397215632042435 29.533208387021205 30.710480629533603 20.35909535140276 52-53 19.315088491011036 29.43110673643613 31.15984153264368 20.093963239909158 54-55 19.25625765980919 29.661403652931313 31.040868633288987 20.041470053970514 56-57 19.100788665318134 29.180704186692168 31.17719356829534 20.54131357969436 58-59 18.519954403920423 29.38468894837048 31.850907113722705 20.24444953398639 60-61 19.247428664339076 29.462008220581527 31.040431554305314 20.250131560774083 62-63 18.896978735233287 28.66145435409342 31.46098895239161 20.980577958281685 64-65 18.971850365135783 28.769238031466188 31.461731986663843 20.797179616734184 66-67 19.279160598553442 28.929252647387404 30.755980551733543 21.03560620232561 68-69 18.938457530783474 28.678850097643444 30.898686839901462 21.484005531671617 70-71 19.165957141783178 28.49164916893802 31.263822622858534 21.07857106642027 72-73 19.050743121688036 28.461315887471393 31.277896566132675 21.2100444247079 74-75 19.542151023027067 28.27026866370968 30.513401715797254 21.67417859746599 76 19.449228031099043 27.95819426937003 30.613536510955825 21.979041188575106 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 1.0 2 1.5 3 1.0 4 0.0 5 1.0 6 4.0 7 6.5 8 7.0 9 18.5 10 42.5 11 89.0 12 123.0 13 195.0 14 389.0 15 738.0 16 1277.5 17 1590.0 18 2202.5 19 3626.5 20 5592.0 21 6746.0 22 8063.5 23 11284.5 24 15201.0 25 17214.0 26 19538.0 27 24346.5 28 29013.5 29 31196.0 30 33896.0 31 38962.0 32 43136.0 33 44944.0 34 46798.5 35 51026.0 36 55043.0 37 56687.0 38 58499.5 39 61441.5 40 63512.5 41 63882.5 42 63311.0 43 63167.0 44 62225.5 45 58832.0 46 56236.0 47 53348.5 48 47358.5 49 41524.5 50 38793.0 51 37416.0 52 31834.5 53 25013.5 54 22397.0 55 19929.0 56 15676.0 57 12100.0 58 10309.0 59 9427.5 60 7468.5 61 5512.0 62 4633.0 63 4104.5 64 3085.5 65 2227.5 66 1621.0 67 1382.0 68 1226.5 69 929.0 70 679.5 71 572.0 72 465.0 73 307.0 74 199.0 75 142.0 76 105.0 77 57.5 78 37.0 79 27.0 80 21.5 81 12.0 82 9.5 83 11.0 84 10.0 85 9.5 86 11.0 87 12.0 88 10.0 89 8.5 90 11.0 91 11.0 92 9.0 93 9.0 94 11.5 95 11.0 96 8.0 97 7.5 98 9.0 99 47.0 100 83.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 1143958.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.33255878991984 #Duplication Level Percentage of deduplicated Percentage of total 1 96.47563873705386 88.1136694674704 2 2.485100584028109 4.539411903792228 3 0.37806734871231606 1.035895750584502 4 0.15084622329858424 0.551086862306213 5 0.11559755675574657 0.5278910324182661 6 0.0874636342005154 0.47929665075591693 7 0.04949497799513511 0.3164352091282527 8 0.05041379173420586 0.3683536477909706 9 0.03411472694104527 0.28042067735505716 >10 0.16383668407349603 2.608485004373282 >50 0.006834332616415446 0.4028340197744228 >100 0.002304290160620668 0.3929477446887554 >500 9.58336787737092E-5 0.07667395948172982 >1k 1.9127875120419918E-4 0.3065980700799745 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG 1842 0.16101989758365254 No Hit GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC 1668 0.145809548951972 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 5.24494780402777E-4 0.0 0.0 2 6.119105771365732E-4 0.0 0.0 3 7.867421706041656E-4 0.0 0.0 4 7.867421706041656E-4 0.0 0.0 5 8.741579673379617E-4 0.0 0.0 6 8.741579673379617E-4 0.0 0.0 7 8.741579673379617E-4 0.0 0.0 8 8.741579673379617E-4 0.0 0.0 9 8.741579673379617E-4 0.0 0.0 10 8.741579673379617E-4 0.0 0.0 11 8.741579673379617E-4 0.0 0.0 12 8.741579673379617E-4 0.0 0.0 13 8.741579673379617E-4 0.0 0.0 14 8.741579673379617E-4 0.0 0.0 15 8.741579673379617E-4 0.0 0.0 16 8.741579673379617E-4 0.0 0.0 17 9.615737640717579E-4 0.0 0.0 18 9.615737640717579E-4 0.0 0.0 19 9.615737640717579E-4 8.741579673379617E-5 0.0 20 9.615737640717579E-4 8.741579673379617E-5 8.741579673379617E-5 21 9.615737640717579E-4 8.741579673379617E-5 2.622473902013885E-4 22 9.615737640717579E-4 8.741579673379617E-5 0.0019231475281435157 23 9.615737640717579E-4 8.741579673379617E-5 0.002097979121611108 24 0.001048989560805554 8.741579673379617E-5 0.0022728107150787004 25 0.0012238211542731465 8.741579673379617E-5 0.002535058105280089 26 0.0012238211542731465 8.741579673379617E-5 0.003059552885682866 27 0.0013112369510069426 8.741579673379617E-5 0.009703153437451375 28 0.0013112369510069426 8.741579673379617E-5 0.02351484932139117 29 0.0014860685444745348 8.741579673379617E-5 0.03173193421436801 30 0.0015734843412083311 1.7483159346759234E-4 0.03986160331061105 31 0.0015734843412083311 1.7483159346759234E-4 0.04702969864278234 32 0.0015734843412083311 2.622473902013885E-4 0.055509030925960566 33 0.0015734843412083311 3.496631869351847E-4 0.06870881623276379 34 0.0015734843412083311 3.496631869351847E-4 0.09676928698431236 35 0.0015734843412083311 4.3707898366898083E-4 0.12684032106073825 36 0.0015734843412083311 4.3707898366898083E-4 0.1781533937434766 37 0.0016609001379421272 5.24494780402777E-4 0.25892558992550424 38 0.0016609001379421272 5.24494780402777E-4 0.34546722869196245 39 0.0016609001379421272 6.993263738703693E-4 0.45351315345493454 40 0.0016609001379421272 6.993263738703693E-4 0.5962631495212237 41 0.0016609001379421272 6.993263738703693E-4 0.7656749635913207 42 0.0016609001379421272 6.993263738703693E-4 0.9533566791787811 43 0.0016609001379421272 6.993263738703693E-4 1.1743438133218178 44 0.0016609001379421272 6.993263738703693E-4 1.4283741186302295 45 0.0016609001379421272 6.993263738703693E-4 1.7229653536231224 46 0.0017483159346759233 6.993263738703693E-4 2.048239533269578 47 0.0017483159346759233 6.993263738703693E-4 2.356205385162742 48 0.0017483159346759233 7.867421706041656E-4 2.6300790763297255 49 0.0017483159346759233 7.867421706041656E-4 2.9058759150248523 50 0.0018357317314097196 7.867421706041656E-4 3.1909388281737616 51 0.0018357317314097196 7.867421706041656E-4 3.5172619973810226 52 0.0018357317314097196 7.867421706041656E-4 3.903902066334603 53 0.0018357317314097196 7.867421706041656E-4 4.296486409466082 54 0.0018357317314097196 7.867421706041656E-4 4.741695062231305 55 0.0019231475281435157 7.867421706041656E-4 5.213652948797071 56 0.0019231475281435157 7.867421706041656E-4 5.67809307684373 57 0.0019231475281435157 7.867421706041656E-4 6.12067925570694 58 0.0019231475281435157 7.867421706041656E-4 6.550415312450283 59 0.0019231475281435157 7.867421706041656E-4 6.9503425825074 60 0.0019231475281435157 7.867421706041656E-4 7.356476374132617 61 0.0019231475281435157 7.867421706041656E-4 7.756403644189734 62 0.002010563324877312 7.867421706041656E-4 8.198727575662742 63 0.002097979121611108 7.867421706041656E-4 8.69052884808708 64 0.0021853949183449043 7.867421706041656E-4 9.263626811473848 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGTGCG 155 1.9845893E-5 18.064516 9 CCCGACC 175 2.7961596E-6 18.0 13 TCTATAC 235 7.934432E-9 17.872341 3 TGCGCGA 120 0.001341063 17.500002 10 TCCTAGC 140 1.8656679E-4 17.5 2 CCGTGCA 205 6.670307E-7 17.073172 9 CTGCGCG 130 0.002290788 16.153847 9 ACGAGTG 1095 0.0 15.981734 70 ACCCCGA 265 3.4942786E-8 15.849057 11 GTCCTAT 200 9.963465E-6 15.750001 1 ACCTGCG 135 0.0029465945 15.555555 7 ACTGCGC 180 7.1508046E-5 15.555554 8 CTGACAC 455 0.0 15.384615 3 CTGGGCG 160 5.162094E-4 15.3125 9 AGTGTCC 325 1.722583E-9 15.076922 8 GTCCTAG 165 6.520095E-4 14.848486 1 TTACACT 425 1.8189894E-12 14.823529 4 GTGTCAG 570 0.0 14.736842 1 CTAAGGT 145 0.004739885 14.4827585 4 GTGCGAT 145 0.004739885 14.4827585 11 >>END_MODULE