FastQCFastQC Report
Fri 12 Jun 2015
000000000-AFM8U l01n02 ve17.3420000000c886.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AFM8U l01n02 ve17.3420000000c886.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143958
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG18420.16101989758365254No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC16680.145809548951972No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGCG1551.9845893E-518.0645169
CCCGACC1752.7961596E-618.013
TCTATAC2357.934432E-917.8723413
TGCGCGA1200.00134106317.50000210
TCCTAGC1401.8656679E-417.52
CCGTGCA2056.670307E-717.0731729
CTGCGCG1300.00229078816.1538479
ACGAGTG10950.015.98173470
ACCCCGA2653.4942786E-815.84905711
GTCCTAT2009.963465E-615.7500011
ACCTGCG1350.002946594515.5555557
ACTGCGC1807.1508046E-515.5555548
CTGACAC4550.015.3846153
CTGGGCG1605.162094E-415.31259
AGTGTCC3251.722583E-915.0769228
GTCCTAG1656.520095E-414.8484861
TTACACT4251.8189894E-1214.8235294
GTGTCAG5700.014.7368421
CTAAGGT1450.00473988514.48275854
GTGCGAT1450.00473988514.482758511
Produced by FastQC (version 0.11.2)